AlfalfaGEDB | MS.gene70134

M. sativa cultivar XinJiangDaYe / MS.gene70134

KEGG: -
GO: -
PFAM: PF05678.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70134.t1	XP_003630432.2	98.4	192	3	0	1	192	1	192	7.80E-99	369.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70134.t1	Q9FNP0	57.0	179	55	6	16	191	13	172	7.8e-39	161.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70134.t1	G7LGI0	98.4	192	3	0	1	192	1	192	5.6e-99	369.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049222	MS.gene70134	0.833878	4.14E-56	-1.69E-46
MS.gene049232	MS.gene70134	0.808242	3.41E-50	-1.69E-46
MS.gene049609	MS.gene70134	0.832209	1.08E-55	-1.69E-46
MS.gene049805	MS.gene70134	0.808241	3.41E-50	-1.69E-46
MS.gene050323	MS.gene70134	0.812038	5.18E-51	-1.69E-46
MS.gene050593	MS.gene70134	0.81862	1.78E-52	-1.69E-46
MS.gene050747	MS.gene70134	0.824328	8.57E-54	-1.69E-46
MS.gene050772	MS.gene70134	0.84129	5.20E-58	-1.69E-46
MS.gene05080	MS.gene70134	0.800563	1.36E-48	-1.69E-46
MS.gene050950	MS.gene70134	0.818676	1.73E-52	-1.69E-46
MS.gene052916	MS.gene70134	0.828696	7.79E-55	-1.69E-46
MS.gene052966	MS.gene70134	0.800875	1.17E-48	-1.69E-46
MS.gene053203	MS.gene70134	0.803576	3.26E-49	-1.69E-46
MS.gene053213	MS.gene70134	0.804242	2.37E-49	-1.69E-46
MS.gene053574	MS.gene70134	0.841148	5.67E-58	-1.69E-46
MS.gene053626	MS.gene70134	0.80447	2.13E-49	-1.69E-46
MS.gene053824	MS.gene70134	0.821001	5.09E-53	-1.69E-46
MS.gene054003	MS.gene70134	0.814675	1.37E-51	-1.69E-46
MS.gene055502	MS.gene70134	0.803628	3.18E-49	-1.69E-46
MS.gene055577	MS.gene70134	0.838267	3.18E-57	-1.69E-46
MS.gene055722	MS.gene70134	0.819949	8.88E-53	-1.69E-46
MS.gene055941	MS.gene70134	0.810512	1.11E-50	-1.69E-46
MS.gene056448	MS.gene70134	0.819087	1.40E-52	-1.69E-46
MS.gene056511	MS.gene70134	0.800121	1.67E-48	-1.69E-46
MS.gene057076	MS.gene70134	0.811971	5.36E-51	-1.69E-46
MS.gene057416	MS.gene70134	0.801449	8.96E-49	-1.69E-46
MS.gene05837	MS.gene70134	0.817409	3.35E-52	-1.69E-46
MS.gene058838	MS.gene70134	0.804899	1.73E-49	-1.69E-46
MS.gene05913	MS.gene70134	0.80494	1.70E-49	-1.69E-46
MS.gene059718	MS.gene70134	0.804481	2.12E-49	-1.69E-46
MS.gene059801	MS.gene70134	0.821554	3.80E-53	-1.69E-46
MS.gene060482	MS.gene70134	0.808809	2.58E-50	-1.69E-46
MS.gene060835	MS.gene70134	0.801109	1.05E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70134.t1	MTR_8g095470	98.438	192	3	0	1	192	1	192	5.91e-138	383
MS.gene70134.t1	MTR_4g088695	34.054	185	94	6	20	188	61	233	8.13e-13	65.1
MS.gene70134.t1	MTR_2g013950	34.826	201	93	10	7	188	71	252	6.28e-12	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70134.t1	AT5G08480	56.983	179	55	6	16	191	13	172	6.51e-46	149
MS.gene70134.t1	AT5G08480	56.983	179	55	6	16	191	13	172	6.51e-46	149
MS.gene70134.t1	AT5G08480	47.486	179	41	5	16	191	13	141	7.22e-31	110
MS.gene70134.t1	AT5G53830	32.828	198	95	5	20	186	49	239	4.24e-12	63.2
MS.gene70134.t1	AT1G28280	33.810	210	87	11	11	188	49	238	8.67e-11	59.7
MS.gene70134.t1	AT1G28280	33.810	210	87	11	11	188	49	238	8.71e-11	59.7

Find 49 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACACAAACACAGGTGCATTT+AGG	0.187922	4.1:+7362023	MS.gene70134:CDS
GTTCTCTCAGTTAACTATTA+TGG	0.217006	4.1:+7362327	MS.gene70134:CDS
TTCCTTCTTTCATGGAGTTT+TGG	0.237618	4.1:-7362139	None:intergenic
GGAAACAAGTTAAGCAATTC+TGG	0.246716	4.1:-7362475	None:intergenic
TCATGCATTTCCTTCTTTCA+TGG	0.261349	4.1:-7362147	None:intergenic
GGTGGTGTTCTCTTCGTAAT+AGG	0.264699	4.1:-7362118	None:intergenic
GGTTTAACAATCTCAAGTTT+TGG	0.319524	4.1:-7362172	None:intergenic
CACAAACACAGGTGCATTTA+GGG	0.321361	4.1:+7362024	MS.gene70134:CDS
TTTCCTTCAAGTCCTGTATC+AGG	0.338299	4.1:+7362256	MS.gene70134:CDS
GAAGTAGTGCAAAGATTAAC+AGG	0.361566	4.1:+7362046	MS.gene70134:CDS
TGTTTGTGATATTGAAGATT+TGG	0.376002	4.1:-7362193	None:intergenic
TAGGTGCAACCTTTGTAGTT+TGG	0.379631	4.1:-7362099	None:intergenic
GTGTGAACAAATGTTGTTAG+TGG	0.401194	4.1:-7362004	None:intergenic
CTTTGTTGCATTGCCTTCTG+AGG	0.427730	4.1:-7362071	None:intergenic
CCAGTACATGGTTCAACAAC+AGG	0.437636	4.1:+7361971	MS.gene70134:CDS
CATACTTTCTCACATGCCTT+AGG	0.439034	4.1:-7362508	None:intergenic
TTTGTTGCATTGCCTTCTGA+GGG	0.457769	4.1:-7362070	None:intergenic
GTTTCCACTGGCATCTCCTA+AGG	0.476906	4.1:+7362492	MS.gene70134:CDS
GAATTTCTTGACTTAGAAGA+AGG	0.481290	4.1:-7362229	None:intergenic
CTTGCTTTAGACCTGGGTGA+TGG	0.493410	4.1:-7362439	None:intergenic
GAACTTCCACTACCTGATAC+AGG	0.493636	4.1:-7362268	None:intergenic
GTGGAAGGTGTGGTAGGGCT+TGG	0.499380	4.1:-7362301	None:intergenic
CTACCTGATACAGGACTTGA+AGG	0.504395	4.1:-7362259	None:intergenic
ATTGCTTAACTTGTTTCCAC+TGG	0.509456	4.1:+7362480	MS.gene70134:CDS
GAAGCCACTTGCTTTAGACC+TGG	0.517471	4.1:-7362446	None:intergenic
TCAAGTCCTGTATCAGGTAG+TGG	0.519505	4.1:+7362262	MS.gene70134:CDS
AAGCCACTTGCTTTAGACCT+GGG	0.539510	4.1:-7362445	None:intergenic
CACCAAAACTCCATGAAAGA+AGG	0.543366	4.1:+7362137	MS.gene70134:CDS
CTCAGAAGGCAATGCAACAA+AGG	0.546327	4.1:+7362072	MS.gene70134:CDS
ATGGAGAAGCCACCAGTACA+TGG	0.555074	4.1:+7361959	MS.gene70134:CDS
CCTGTTGTTGAACCATGTAC+TGG	0.565708	4.1:-7361971	None:intergenic
AACAATGTGGAAGGTGTGGT+AGG	0.566267	4.1:-7362307	None:intergenic
AAATCTGATTGTGATGGGAA+TGG	0.567485	4.1:+7361940	None:intergenic
AGATTAACAGGTCCCTCAGA+AGG	0.568541	4.1:+7362058	MS.gene70134:CDS
ACAATGTGGAAGGTGTGGTA+GGG	0.605585	4.1:-7362306	None:intergenic
GTTGTTGAACCATGTACTGG+TGG	0.616693	4.1:-7361968	None:intergenic
TGGAAGATGAAAAGAAACAA+GGG	0.617549	4.1:+7362347	MS.gene70134:CDS
ACATTTGTTCACACAAACAC+AGG	0.619702	4.1:+7362013	MS.gene70134:CDS
ATGGAAGATGAAAAGAAACA+AGG	0.623994	4.1:+7362346	MS.gene70134:CDS
AACTGAGAGAACAATGTGGA+AGG	0.625030	4.1:-7362316	None:intergenic
CTTCAATATCACAAACAACC+AGG	0.631097	4.1:+7362199	MS.gene70134:CDS
TCACCCAGGTCTAAAGCAAG+TGG	0.631290	4.1:+7362442	MS.gene70134:CDS
AGAAAGCTATCAAAGAGAGA+CGG	0.633848	4.1:+7362404	MS.gene70134:CDS
TTTATTTGCATCCATCACCC+AGG	0.643004	4.1:+7362428	MS.gene70134:CDS
TTAGAAGAAGGTGATGCACC+TGG	0.665275	4.1:-7362217	None:intergenic
AAAGGAAGACCAAACTACAA+AGG	0.666842	4.1:+7362090	MS.gene70134:CDS
AGAGAACAATGTGGAAGGTG+TGG	0.689227	4.1:-7362311	None:intergenic
CATGCCTTAGGAGATGCCAG+TGG	0.723253	4.1:-7362496	None:intergenic
AGTTAACTGAGAGAACAATG+TGG	0.758270	4.1:-7362320	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGTTTGTGATATTGAAGATT+TGG	-	chr4.1:7362196-7362215	None:intergenic	25.0%
	ATGGAAGATGAAAAGAAACA+AGG	+	chr4.1:7362346-7362365	MS.gene70134:CDS	30.0%
	GAATTTCTTGACTTAGAAGA+AGG	-	chr4.1:7362232-7362251	None:intergenic	30.0%
	GTTCTCTCAGTTAACTATTA+TGG	+	chr4.1:7362327-7362346	MS.gene70134:CDS	30.0%
	TGGAAGATGAAAAGAAACAA+GGG	+	chr4.1:7362347-7362366	MS.gene70134:CDS	30.0%
!	GGTTTAACAATCTCAAGTTT+TGG	-	chr4.1:7362175-7362194	None:intergenic	30.0%
	AAAGGAAGACCAAACTACAA+AGG	+	chr4.1:7362090-7362109	MS.gene70134:CDS	35.0%
	ACATTTGTTCACACAAACAC+AGG	+	chr4.1:7362013-7362032	MS.gene70134:CDS	35.0%
	AGAAAGCTATCAAAGAGAGA+CGG	+	chr4.1:7362404-7362423	MS.gene70134:CDS	35.0%
	AGTTAACTGAGAGAACAATG+TGG	-	chr4.1:7362323-7362342	None:intergenic	35.0%
	CTTCAATATCACAAACAACC+AGG	+	chr4.1:7362199-7362218	MS.gene70134:CDS	35.0%
	GAAGTAGTGCAAAGATTAAC+AGG	+	chr4.1:7362046-7362065	MS.gene70134:CDS	35.0%
	GGAAACAAGTTAAGCAATTC+TGG	-	chr4.1:7362478-7362497	None:intergenic	35.0%
	GTGTGAACAAATGTTGTTAG+TGG	-	chr4.1:7362007-7362026	None:intergenic	35.0%
	TCATGCATTTCCTTCTTTCA+TGG	-	chr4.1:7362150-7362169	None:intergenic	35.0%
!	TTCCTTCTTTCATGGAGTTT+TGG	-	chr4.1:7362142-7362161	None:intergenic	35.0%
!!	ATTGCTTAACTTGTTTCCAC+TGG	+	chr4.1:7362480-7362499	MS.gene70134:CDS	35.0%
	AACTGAGAGAACAATGTGGA+AGG	-	chr4.1:7362319-7362338	None:intergenic	40.0%
	ACACAAACACAGGTGCATTT+AGG	+	chr4.1:7362023-7362042	MS.gene70134:CDS	40.0%
	CACAAACACAGGTGCATTTA+GGG	+	chr4.1:7362024-7362043	MS.gene70134:CDS	40.0%
	CACCAAAACTCCATGAAAGA+AGG	+	chr4.1:7362137-7362156	MS.gene70134:CDS	40.0%
	CATACTTTCTCACATGCCTT+AGG	-	chr4.1:7362511-7362530	None:intergenic	40.0%
	TAGGTGCAACCTTTGTAGTT+TGG	-	chr4.1:7362102-7362121	None:intergenic	40.0%
	TTTATTTGCATCCATCACCC+AGG	+	chr4.1:7362428-7362447	MS.gene70134:CDS	40.0%
	TTTCCTTCAAGTCCTGTATC+AGG	+	chr4.1:7362256-7362275	MS.gene70134:CDS	40.0%
	TTTGTTGCATTGCCTTCTGA+GGG	-	chr4.1:7362073-7362092	None:intergenic	40.0%
!!	CTTCTTTCATGGAGTTTTGG+TGG	-	chr4.1:7362139-7362158	None:intergenic	40.0%
	AGAGAACAATGTGGAAGGTG+TGG	-	chr4.1:7362314-7362333	None:intergenic	45.0%
	AGATTAACAGGTCCCTCAGA+AGG	+	chr4.1:7362058-7362077	MS.gene70134:CDS	45.0%
	CCAGTACATGGTTCAACAAC+AGG	+	chr4.1:7361971-7361990	MS.gene70134:CDS	45.0%
	CCTGTTGTTGAACCATGTAC+TGG	-	chr4.1:7361974-7361993	None:intergenic	45.0%
	CTACCTGATACAGGACTTGA+AGG	-	chr4.1:7362262-7362281	None:intergenic	45.0%
	CTCAGAAGGCAATGCAACAA+AGG	+	chr4.1:7362072-7362091	MS.gene70134:CDS	45.0%
	CTTTGTTGCATTGCCTTCTG+AGG	-	chr4.1:7362074-7362093	None:intergenic	45.0%
	GAACTTCCACTACCTGATAC+AGG	-	chr4.1:7362271-7362290	None:intergenic	45.0%
	GGTGGTGTTCTCTTCGTAAT+AGG	-	chr4.1:7362121-7362140	None:intergenic	45.0%
	GTTGTTGAACCATGTACTGG+TGG	-	chr4.1:7361971-7361990	None:intergenic	45.0%
	TCAAGTCCTGTATCAGGTAG+TGG	+	chr4.1:7362262-7362281	MS.gene70134:CDS	45.0%
	TTAGAAGAAGGTGATGCACC+TGG	-	chr4.1:7362220-7362239	None:intergenic	45.0%
!	AACAATGTGGAAGGTGTGGT+AGG	-	chr4.1:7362310-7362329	None:intergenic	45.0%
!	AAGCCACTTGCTTTAGACCT+GGG	-	chr4.1:7362448-7362467	None:intergenic	45.0%
!	ACAATGTGGAAGGTGTGGTA+GGG	-	chr4.1:7362309-7362328	None:intergenic	45.0%
	ATGGAGAAGCCACCAGTACA+TGG	+	chr4.1:7361959-7361978	MS.gene70134:CDS	50.0%
	GTTTCCACTGGCATCTCCTA+AGG	+	chr4.1:7362492-7362511	MS.gene70134:CDS	50.0%
!	CTTGCTTTAGACCTGGGTGA+TGG	-	chr4.1:7362442-7362461	None:intergenic	50.0%
!	GAAGCCACTTGCTTTAGACC+TGG	-	chr4.1:7362449-7362468	None:intergenic	50.0%
!	TCACCCAGGTCTAAAGCAAG+TGG	+	chr4.1:7362442-7362461	MS.gene70134:CDS	50.0%
	CATGCCTTAGGAGATGCCAG+TGG	-	chr4.1:7362499-7362518	None:intergenic	55.0%
!	GTGGAAGGTGTGGTAGGGCT+TGG	-	chr4.1:7362304-7362323	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	7361953	7362531	7361953	ID=MS.gene70134
chr4.1	mRNA	7361953	7362531	7361953	ID=MS.gene70134.t1;Parent=MS.gene70134
chr4.1	exon	7361953	7362531	7361953	ID=MS.gene70134.t1.exon1;Parent=MS.gene70134.t1
chr4.1	CDS	7361953	7362531	7361953	ID=cds.MS.gene70134.t1;Parent=MS.gene70134.t1

Gene Sequence

>MS.gene70134

ATGGGAATGGAGAAGCCACCAGTACATGGTTCAACAACAGGTGATTGTAAACCACTAACAACATTTGTTCACACAAACACAGGTGCATTTAGGGAAGTAGTGCAAAGATTAACAGGTCCCTCAGAAGGCAATGCAACAAAGGAAGACCAAACTACAAAGGTTGCACCTATTACGAAGAGAACACCACCAAAACTCCATGAAAGAAGGAAATGCATGAAGCCAAAACTTGAGATTGTTAAACCAAATCTTCAATATCACAAACAACCAGGTGCATCACCTTCTTCTAAGTCAAGAAATTCTAGCTTTCCTTCAAGTCCTGTATCAGGTAGTGGAAGTTCTAGTCTTCTTCCAAGCCCTACCACACCTTCCACATTGTTCTCTCAGTTAACTATTATGGAAGATGAAAAGAAACAAGGGTCTGTGATAAATGAAATAAACATAGAAGAAGAAGAGAAAGCTATCAAAGAGAGACGGTTTTATTTGCATCCATCACCCAGGTCTAAAGCAAGTGGCTTCAATGAACCAGAATTGCTTAACTTGTTTCCACTGGCATCTCCTAAGGCATGTGAGAAAGTATGA

Protein sequence

>MS.gene70134.t1

MGMEKPPVHGSTTGDCKPLTTFVHTNTGAFREVVQRLTGPSEGNATKEDQTTKVAPITKRTPPKLHERRKCMKPKLEIVKPNLQYHKQPGASPSSKSRNSSFPSSPVSGSGSSSLLPSPTTPSTLFSQLTIMEDEKKQGSVINEINIEEEEKAIKERRFYLHPSPRSKASGFNEPELLNLFPLASPKACEKV