AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70811


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70811.t1 MTR_1g081260 99.670 303 1 0 1 303 1 303 0.0 620
MS.gene70811.t1 MTR_7g114790 72.026 311 72 7 1 302 1 305 1.53e-156 439
MS.gene70811.t1 MTR_7g095100 64.725 309 101 4 1 303 1 307 2.74e-144 408
MS.gene70811.t1 MTR_3g076610 35.507 276 152 3 4 257 7 278 1.94e-61 198
MS.gene70811.t1 MTR_5g069570 37.647 255 155 3 10 260 14 268 2.19e-60 195
MS.gene70811.t1 MTR_4g104760 36.122 263 152 6 4 253 7 266 2.63e-59 192
MS.gene70811.t1 MTR_3g082430 40.773 233 133 3 26 253 12 244 6.50e-58 188
MS.gene70811.t1 MTR_4g055230 36.760 321 166 10 1 301 1 304 1.28e-57 187
MS.gene70811.t1 MTR_2g084965 38.433 268 146 7 6 254 8 275 4.34e-50 168
MS.gene70811.t1 MTR_7g103900 33.129 326 177 10 1 302 1 309 3.23e-49 166
MS.gene70811.t1 MTR_3g106250 36.466 266 142 8 4 253 6 260 3.65e-49 167
MS.gene70811.t1 MTR_5g096570 33.910 289 175 5 23 301 1178 1460 1.11e-48 174
MS.gene70811.t1 MTR_8g069535 80.488 82 15 1 8 89 9 89 1.15e-32 121
MS.gene70811.t1 MTR_8g069535 66.102 59 20 0 176 234 89 147 6.21e-17 79.0
MS.gene70811.t1 MTR_1g013100 29.643 280 154 13 4 261 8 266 1.29e-32 123
MS.gene70811.t1 MTR_7g103900 30.043 233 138 7 1 212 1 229 2.29e-28 109
MS.gene70811.t1 MTR_5g069550 32.143 168 98 2 10 175 14 167 1.55e-24 98.2
MS.gene70811.t1 MTR_0716s0040 58.228 79 11 1 195 273 15 71 1.17e-22 89.7
MS.gene70811.t1 MTR_0716s0020 71.154 52 11 1 56 107 3 50 3.62e-16 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70811.t1 AT5G28150 65.789 304 88 4 1 303 1 289 1.66e-139 395
MS.gene70811.t1 AT3G04860 64.379 306 89 6 1 303 1 289 4.65e-135 384
MS.gene70811.t1 AT2G04220 41.200 250 142 3 14 259 12 260 2.02e-65 207
MS.gene70811.t1 AT4G12690 41.975 243 135 4 15 253 9 249 3.14e-64 204
MS.gene70811.t1 AT4G12690 41.975 243 135 4 15 253 9 249 3.14e-64 204
MS.gene70811.t1 AT5G48270 36.260 262 149 9 5 253 13 269 7.46e-52 173
MS.gene70811.t1 AT2G27770 33.433 335 173 10 1 301 1 319 2.00e-49 167
MS.gene70811.t1 AT3G13229 34.894 235 142 4 33 256 8 242 3.79e-45 155
MS.gene70811.t1 AT5G11000 37.302 252 117 9 31 253 33 272 7.76e-43 151
MS.gene70811.t1 AT2G25200 28.571 259 147 8 6 242 16 258 1.32e-30 118
MS.gene70811.t1 AT2G36470 26.471 272 145 8 1 241 1 248 9.25e-20 88.2

Find 42 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCTCTCAAGATAAAATT+TGG 0.088174 1.2:+61124196 MS.gene70811:CDS
AAACAAATGGTTCTTCTTCT+TGG 0.224208 1.2:+61123814 MS.gene70811:CDS
TTTATTGGGATGTTTATAAT+TGG 0.245969 1.2:+61124127 MS.gene70811:CDS
GTCTTGAAGTTGAGGTTTAT+TGG 0.251621 1.2:+61124112 MS.gene70811:CDS
AACACCTTGTTGCTGCAATT+TGG 0.305444 1.2:-61124283 None:intergenic
GATCTTTCTTCAGCTAGATT+TGG 0.328970 1.2:+61123742 MS.gene70811:CDS
GTTTACCAGTGTTCAATTAG+AGG 0.364536 1.2:+61123538 MS.gene70811:CDS
TCTTGAAGTTGAGGTTTATT+GGG 0.375109 1.2:+61124113 MS.gene70811:CDS
AAAACATTTGCATCAGAAAC+AGG 0.388646 1.2:-61124228 None:intergenic
TTTCTCTCAAGATAAAATTT+GGG 0.389101 1.2:+61124197 MS.gene70811:CDS
AGGTTGAAGTGGAAGTTTAG+AGG 0.396077 1.2:+61124066 MS.gene70811:CDS
TTCAAGACCATCCACAAGTA+TGG 0.397921 1.2:-61124097 None:intergenic
AAGCAAAACTGCTCAGAATC+TGG 0.403487 1.2:+61123507 MS.gene70811:CDS
GTTGTAAGATTGATGTGTAT+TGG 0.407371 1.2:+61123719 MS.gene70811:CDS
CTTGCTTGATTACTATTACA+TGG 0.414580 1.2:+61123566 MS.gene70811:CDS
GTGATCCTTGTCTTATAGTT+CGG 0.427025 1.2:+61124013 MS.gene70811:CDS
TTGTAAGATTGATGTGTATT+GGG 0.428969 1.2:+61123720 MS.gene70811:CDS
TCTTGGTGATTTAAGAAAAG+AGG 0.454695 1.2:+61123831 MS.gene70811:CDS
TGTAGTTGTTGATAAACAAA+TGG 0.479417 1.2:+61123801 MS.gene70811:CDS
TAAAGTTGATATAAAACCTT+GGG 0.481774 1.2:+61123654 MS.gene70811:CDS
TACATGGAGTAAGAGTTTGA+TGG 0.485899 1.2:+61123582 MS.gene70811:CDS
TCTTCAGCTAGATTTGGTGT+TGG 0.510529 1.2:+61123748 MS.gene70811:CDS
TGTGGATGGTCTTGAAGTTG+AGG 0.522288 1.2:+61124104 MS.gene70811:CDS
AAAACAGCATTCAAAGGTAA+AGG 0.522755 1.2:-61123877 None:intergenic
CGATAGCAAAACTGTGATGC+AGG 0.523931 1.2:+61124038 MS.gene70811:CDS
AGTAAGAGTTTGATGGGTCA+AGG 0.525556 1.2:+61123589 MS.gene70811:CDS
ACATGGAGTAAGAGTTTGAT+GGG 0.527449 1.2:+61123583 MS.gene70811:CDS
TGCAGTGGTCTTTCTCTCAG+AGG 0.536201 1.2:+61124250 MS.gene70811:CDS
AAACTGTGATGCAGGTGAAA+AGG 0.551305 1.2:+61124046 MS.gene70811:CDS
TGTTGGACCTGAACCATTGG+AGG 0.554106 1.2:+61123765 MS.gene70811:CDS
TGGTGTTGGACCTGAACCAT+TGG 0.554528 1.2:+61123762 MS.gene70811:CDS
GTAAAGTTGATATAAAACCT+TGG 0.555894 1.2:+61123653 MS.gene70811:CDS
AAATTGCAGCAACAAGGTGT+TGG 0.560217 1.2:+61124285 MS.gene70811:CDS
TGCAGGTGAAAAGGTTGAAG+TGG 0.566657 1.2:+61124055 MS.gene70811:CDS
AGGAAATCATACCATACTTG+TGG 0.576851 1.2:+61124086 MS.gene70811:CDS
GCATTCTCACCAAAACAAGA+TGG 0.592596 1.2:-61123445 None:intergenic
GTTGGACCTGAACCATTGGA+GGG 0.614502 1.2:+61123766 MS.gene70811:CDS
AATCATACCATACTTGTGGA+TGG 0.616419 1.2:+61124090 MS.gene70811:CDS
ATCTACCTCTAATTGAACAC+TGG 0.618529 1.2:-61123543 None:intergenic
GAGACCAAATTGCAGCAACA+AGG 0.620010 1.2:+61124279 MS.gene70811:CDS
TTTCTCTTAGAAAACACCCA+AGG 0.637931 1.2:-61123670 None:intergenic
TCGATCCGAACTATAAGACA+AGG 0.676375 1.2:-61124018 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTGGGATGTTTATAAT+TGG + chr1.2:61124127-61124146 MS.gene70811:CDS 20.0%
!! TTTCTCTCAAGATAAAATTT+GGG + chr1.2:61124197-61124216 MS.gene70811:CDS 20.0%
!!! TAAAGTTGATATAAAACCTT+GGG + chr1.2:61123654-61123673 MS.gene70811:CDS 20.0%
! GTTTCTCTCAAGATAAAATT+TGG + chr1.2:61124196-61124215 MS.gene70811:CDS 25.0%
! TTGTAAGATTGATGTGTATT+GGG + chr1.2:61123720-61123739 MS.gene70811:CDS 25.0%
!! TGTAGTTGTTGATAAACAAA+TGG + chr1.2:61123801-61123820 MS.gene70811:CDS 25.0%
!!! GATGTTTATAATTGGCTTTT+TGG + chr1.2:61124135-61124154 MS.gene70811:CDS 25.0%
!!! GTAAAGTTGATATAAAACCT+TGG + chr1.2:61123653-61123672 MS.gene70811:CDS 25.0%
!!! GTTTTTGGTAAGAAGTTTTT+TGG + chr1.2:61123919-61123938 MS.gene70811:CDS 25.0%
!!! TAAGAAGTTTTTTGGTAACA+AGG + chr1.2:61123927-61123946 MS.gene70811:CDS 25.0%
AAAACAGCATTCAAAGGTAA+AGG - chr1.2:61123880-61123899 None:intergenic 30.0%
AAAACATTTGCATCAGAAAC+AGG - chr1.2:61124231-61124250 None:intergenic 30.0%
AAACAAATGGTTCTTCTTCT+TGG + chr1.2:61123814-61123833 MS.gene70811:CDS 30.0%
CTTGCTTGATTACTATTACA+TGG + chr1.2:61123566-61123585 MS.gene70811:CDS 30.0%
GTTGTAAGATTGATGTGTAT+TGG + chr1.2:61123719-61123738 MS.gene70811:CDS 30.0%
TCTTGAAGTTGAGGTTTATT+GGG + chr1.2:61124113-61124132 MS.gene70811:CDS 30.0%
! GCTAAGAAAGAACATGTTTT+TGG + chr1.2:61123904-61123923 MS.gene70811:CDS 30.0%
! TCTTGGTGATTTAAGAAAAG+AGG + chr1.2:61123831-61123850 MS.gene70811:CDS 30.0%
!! AAGGATTTTCCATCTTGTTT+TGG + chr1.2:61123436-61123455 MS.gene70811:CDS 30.0%
AATCATACCATACTTGTGGA+TGG + chr1.2:61124090-61124109 MS.gene70811:CDS 35.0%
ACATGGAGTAAGAGTTTGAT+GGG + chr1.2:61123583-61123602 MS.gene70811:CDS 35.0%
AGGAAATCATACCATACTTG+TGG + chr1.2:61124086-61124105 MS.gene70811:CDS 35.0%
ATCTACCTCTAATTGAACAC+TGG - chr1.2:61123546-61123565 None:intergenic 35.0%
GATCTTTCTTCAGCTAGATT+TGG + chr1.2:61123742-61123761 MS.gene70811:CDS 35.0%
GCAACAAAAACAGCATTCAA+AGG - chr1.2:61123886-61123905 None:intergenic 35.0%
GTCTTGAAGTTGAGGTTTAT+TGG + chr1.2:61124112-61124131 MS.gene70811:CDS 35.0%
GTGATCCTTGTCTTATAGTT+CGG + chr1.2:61124013-61124032 MS.gene70811:CDS 35.0%
GTTTACCAGTGTTCAATTAG+AGG + chr1.2:61123538-61123557 MS.gene70811:CDS 35.0%
TACATGGAGTAAGAGTTTGA+TGG + chr1.2:61123582-61123601 MS.gene70811:CDS 35.0%
TTTCTCTTAGAAAACACCCA+AGG - chr1.2:61123673-61123692 None:intergenic 35.0%
! AAGGTTGTGTTTTGTGACAA+TGG + chr1.2:61123946-61123965 MS.gene70811:CDS 35.0%
! TCTCACATGTTTTGTATGCT+TGG + chr1.2:61124310-61124329 MS.gene70811:CDS 35.0%
!! TGGCTTTTTGGTACATCTTT+TGG + chr1.2:61124147-61124166 MS.gene70811:CDS 35.0%
!! TGGGTGTTTTCTAAGAGAAA+GGG + chr1.2:61123673-61123692 MS.gene70811:CDS 35.0%
!! TTGGGTGTTTTCTAAGAGAA+AGG + chr1.2:61123672-61123691 MS.gene70811:CDS 35.0%
AAAAAACCCTCCAATGGTTC+AGG - chr1.2:61123775-61123794 None:intergenic 40.0%
AAACTGTGATGCAGGTGAAA+AGG + chr1.2:61124046-61124065 MS.gene70811:CDS 40.0%
AAATTGCAGCAACAAGGTGT+TGG + chr1.2:61124285-61124304 MS.gene70811:CDS 40.0%
AACACCTTGTTGCTGCAATT+TGG - chr1.2:61124286-61124305 None:intergenic 40.0%
AAGCAAAACTGCTCAGAATC+TGG + chr1.2:61123507-61123526 MS.gene70811:CDS 40.0%
AGGTTGAAGTGGAAGTTTAG+AGG + chr1.2:61124066-61124085 MS.gene70811:CDS 40.0%
CCTACAAAAAAACCCTCCAA+TGG - chr1.2:61123781-61123800 None:intergenic 40.0%
GCATTCTCACCAAAACAAGA+TGG - chr1.2:61123448-61123467 None:intergenic 40.0%
TCGATCCGAACTATAAGACA+AGG - chr1.2:61124021-61124040 None:intergenic 40.0%
TTCAAGACCATCCACAAGTA+TGG - chr1.2:61124100-61124119 None:intergenic 40.0%
! AGTAAGAGTTTGATGGGTCA+AGG + chr1.2:61123589-61123608 MS.gene70811:CDS 40.0%
! CTGATGCAAATGTTTTGCAG+TGG + chr1.2:61124235-61124254 MS.gene70811:CDS 40.0%
! TTTTGGTGAGAATGCAGTTC+AGG + chr1.2:61123453-61123472 MS.gene70811:CDS 40.0%
!! ATGGGTCAAGGTTTTAGTGT+TGG + chr1.2:61123601-61123620 MS.gene70811:CDS 40.0%
!! TCTTCAGCTAGATTTGGTGT+TGG + chr1.2:61123748-61123767 MS.gene70811:CDS 40.0%
!! TGGGTCAAGGTTTTAGTGTT+GGG + chr1.2:61123602-61123621 MS.gene70811:CDS 40.0%
!!! CCATTGGAGGGTTTTTTTGT+AGG + chr1.2:61123778-61123797 MS.gene70811:CDS 40.0%
CGATAGCAAAACTGTGATGC+AGG + chr1.2:61124038-61124057 MS.gene70811:CDS 45.0%
GAGACCAAATTGCAGCAACA+AGG + chr1.2:61124279-61124298 MS.gene70811:CDS 45.0%
TGCAGGTGAAAAGGTTGAAG+TGG + chr1.2:61124055-61124074 MS.gene70811:CDS 45.0%
! TGTGGATGGTCTTGAAGTTG+AGG + chr1.2:61124104-61124123 MS.gene70811:CDS 45.0%
GTTGGACCTGAACCATTGGA+GGG + chr1.2:61123766-61123785 MS.gene70811:CDS 50.0%
TGCAGTGGTCTTTCTCTCAG+AGG + chr1.2:61124250-61124269 MS.gene70811:CDS 50.0%
TGTTGGACCTGAACCATTGG+AGG + chr1.2:61123765-61123784 MS.gene70811:CDS 50.0%
! TGGTGTTGGACCTGAACCAT+TGG + chr1.2:61123762-61123781 MS.gene70811:CDS 50.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 61123433 61124344 61123433 ID=MS.gene70811
chr1.2 mRNA 61123433 61124344 61123433 ID=MS.gene70811.t1;Parent=MS.gene70811
chr1.2 exon 61123433 61124344 61123433 ID=MS.gene70811.t1.exon1;Parent=MS.gene70811.t1
chr1.2 CDS 61123433 61124344 61123433 ID=cds.MS.gene70811.t1;Parent=MS.gene70811.t1
Gene Sequence

>MS.gene70811

ATGAAGGATTTTCCATCTTGTTTTGGTGAGAATGCAGTTCAGGTAGCAGATTCATCATCATCATCTTCAAGCTCAAGCAAAACTGCTCAGAATCTGGTTATTTGTGTTTACCAGTGTTCAATTAGAGGTAGATCTTGCTTGATTACTATTACATGGAGTAAGAGTTTGATGGGTCAAGGTTTTAGTGTTGGGATTGATGATGCTTCAAATCAGTGTTTATGTAAAGTTGATATAAAACCTTGGGTGTTTTCTAAGAGAAAGGGTTGTAAGAGTTTAGAAGCTTATAGTTGTAAGATTGATGTGTATTGGGATCTTTCTTCAGCTAGATTTGGTGTTGGACCTGAACCATTGGAGGGTTTTTTTGTAGGTGTAGTTGTTGATAAACAAATGGTTCTTCTTCTTGGTGATTTAAGAAAAGAGGCTTTTAAGAAATCTAATGCAGTTCCTTTACCTTTGAATGCTGTTTTTGTTGCTAAGAAAGAACATGTTTTTGGTAAGAAGTTTTTTGGTAACAAGGTTGTGTTTTGTGACAATGGTAAAATTCATGATCTTGTTATTGAGTGTGATACTTCTGTTGCAAGTGATCCTTGTCTTATAGTTCGGATCGATAGCAAAACTGTGATGCAGGTGAAAAGGTTGAAGTGGAAGTTTAGAGGAAATCATACCATACTTGTGGATGGTCTTGAAGTTGAGGTTTATTGGGATGTTTATAATTGGCTTTTTGGTACATCTTTTGGAAATGCTGTTTTCATGTTTAGAACATGTTTCTCTCAAGATAAAATTTGGGATGCTCAACCTGTTTCTGATGCAAATGTTTTGCAGTGGTCTTTCTCTCAGAGGTTTTCTGAGACCAAATTGCAGCAACAAGGTGTTGGTTTCTCACATGTTTTGTATGCTTGGAAGAATGAATAG

Protein sequence

>MS.gene70811.t1

MKDFPSCFGENAVQVADSSSSSSSSSKTAQNLVICVYQCSIRGRSCLITITWSKSLMGQGFSVGIDDASNQCLCKVDIKPWVFSKRKGCKSLEAYSCKIDVYWDLSSARFGVGPEPLEGFFVGVVVDKQMVLLLGDLRKEAFKKSNAVPLPLNAVFVAKKEHVFGKKFFGNKVVFCDNGKIHDLVIECDTSVASDPCLIVRIDSKTVMQVKRLKWKFRGNHTILVDGLEVEVYWDVYNWLFGTSFGNAVFMFRTCFSQDKIWDAQPVSDANVLQWSFSQRFSETKLQQQGVGFSHVLYAWKNE