AlfalfaGEDB | MS.gene72160

M. sativa cultivar XinJiangDaYe / MS.gene72160

KEGG: K12581
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF04857.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72160.t1	XP_024629048.1	95.6	272	12	0	1	272	1	272	1.40E-149	538.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72160.t1	Q9FMS6	64.7	266	90	3	9	270	12	277	2.9e-92	339.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72160.t1	I3SR57	95.6	272	12	0	1	272	1	272	9.9e-150	538.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051837	MS.gene72160	0.805552	1.26E-49	-1.69E-46
MS.gene051959	MS.gene72160	0.800545	1.37E-48	-1.69E-46
MS.gene052053	MS.gene72160	0.809333	1.99E-50	-1.69E-46
MS.gene052091	MS.gene72160	0.852322	4.98E-61	-1.69E-46
MS.gene053932	MS.gene72160	0.819151	1.35E-52	-1.69E-46
MS.gene055549	MS.gene72160	0.84711	1.42E-59	-1.69E-46
MS.gene055550	MS.gene72160	0.85964	3.60E-63	-1.69E-46
MS.gene055551	MS.gene72160	0.842878	1.98E-58	-1.69E-46
MS.gene056365	MS.gene72160	0.805518	1.28E-49	-1.69E-46
MS.gene057093	MS.gene72160	0.801563	8.49E-49	-1.69E-46
MS.gene057167	MS.gene72160	0.814855	1.25E-51	-1.69E-46
MS.gene058628	MS.gene72160	0.810349	1.20E-50	-1.69E-46
MS.gene058630	MS.gene72160	0.809649	1.70E-50	-1.69E-46
MS.gene060964	MS.gene72160	0.811057	8.47E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene72160	MS.gene41101	PPI
MS.gene72160	MS.gene007154	PPI
MS.gene72160	MS.gene72977	PPI
MS.gene72160	MS.gene025628	PPI
MS.gene72160	MS.gene065708	PPI
MS.gene72160	MS.gene41100	PPI
MS.gene053225	MS.gene72160	PPI
MS.gene72978	MS.gene72160	PPI
MS.gene72160	MS.gene065709	PPI
MS.gene72160	MS.gene065707	PPI
MS.gene72160	MS.gene000609	PPI
MS.gene034815	MS.gene72160	PPI
MS.gene72160	MS.gene034816	PPI
MS.gene72160	MS.gene21541	PPI
MS.gene025629	MS.gene72160	PPI
MS.gene72160	MS.gene41097	PPI
MS.gene034250	MS.gene72160	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72160.t1	AT5G22250	64.662	266	90	3	9	270	12	277	6.40e-120	344
MS.gene72160.t1	AT3G44260	61.132	265	98	3	9	270	17	279	4.51e-116	335
MS.gene72160.t1	AT2G32070	50.746	268	127	5	4	270	5	268	4.63e-92	273
MS.gene72160.t1	AT5G10960	50.562	267	128	4	4	270	5	267	7.14e-91	270
MS.gene72160.t1	AT1G80780	49.064	267	132	4	4	270	5	267	1.27e-88	265
MS.gene72160.t1	AT1G80780	49.064	267	132	4	4	270	5	267	1.27e-88	265
MS.gene72160.t1	AT1G80780	49.064	267	132	4	4	270	5	267	1.73e-88	265
MS.gene72160.t1	AT1G15920	47.407	270	134	5	5	269	10	276	8.64e-81	245
MS.gene72160.t1	AT1G15920	47.407	270	134	5	5	269	10	276	8.64e-81	245
MS.gene72160.t1	AT1G61470	37.037	243	130	7	13	249	4	229	1.14e-38	137
MS.gene72160.t1	AT1G06450	33.700	273	157	9	1	268	1	254	1.51e-38	138
MS.gene72160.t1	AT1G27820	35.510	245	143	5	7	248	2	234	8.44e-34	125
MS.gene72160.t1	AT1G27890	35.565	239	139	5	13	248	3	229	1.60e-32	121
MS.gene72160.t1	AT3G44240	35.242	227	132	7	25	249	3	216	3.77e-32	119

Find 62 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTCACAGCGCTTATGATTT+TGG	0.104590	4.2:+6680487	MS.gene72160:CDS
ATCAAATTCAACCTCTAAAT+TGG	0.207989	4.2:-6680056	None:intergenic
GATGGCAACCTTCCTCATTT+TGG	0.210731	4.2:+6680259	MS.gene72160:CDS
AAACACGCTGGGGTACTATT+TGG	0.216392	4.2:+6680784	MS.gene72160:CDS
GCTGAATTGATGCTCACTTC+TGG	0.258268	4.2:+6680430	MS.gene72160:CDS
CAATCATCATCCGTCAAGTT+TGG	0.286714	4.2:+6680029	MS.gene72160:CDS
TCTTCAACAAATCAGTTGTT+TGG	0.295934	4.2:+6680458	MS.gene72160:CDS
AACGGTTCGGCAGATTCCTT+CGG	0.352418	4.2:-6680531	None:intergenic
CTTAACGTGGGTCGGGTTGC+TGG	0.360050	4.2:+6680670	MS.gene72160:CDS
AGATGACGGCGGTCAACTTT+GGG	0.368577	4.2:-6680151	None:intergenic
CTTCAACAAATCAGTTGTTT+GGG	0.376564	4.2:+6680459	MS.gene72160:CDS
GGACAAATAGCCGCTACATA+TGG	0.395638	4.2:+6680281	MS.gene72160:CDS
ACGGTTCGGCAGATTCCTTC+GGG	0.395658	4.2:-6680530	None:intergenic
AATCATCATCCGTCAAGTTT+GGG	0.414340	4.2:+6680030	MS.gene72160:CDS
AAGATGACGGCGGTCAACTT+TGG	0.418710	4.2:-6680152	None:intergenic
GAATCTGCCGAACCGTTTAG+AGG	0.420121	4.2:+6680537	MS.gene72160:CDS
AGTTTGGGCATCCAATTTAG+AGG	0.424325	4.2:+6680045	MS.gene72160:CDS
TCCATGGATACTGAATTTCC+AGG	0.437255	4.2:+6680115	MS.gene72160:CDS
GGTTGCCATCACCATCAGAT+AGG	0.449581	4.2:-6680246	None:intergenic
CAAACTTGACGGATGATGAT+TGG	0.450788	4.2:-6680028	None:intergenic
TACTCATTTCTTGAATAATG+AGG	0.477666	4.2:+6680756	MS.gene72160:CDS
TGACCACTATCGTTACTTGA+AGG	0.492905	4.2:+6680183	MS.gene72160:CDS
GACAAATAGCCGCTACATAT+GGG	0.500452	4.2:+6680282	MS.gene72160:CDS
CAATTGAGCCTTGGTTGTAA+AGG	0.504025	4.2:-6680339	None:intergenic
TGCGCTTGAAGTCAATCCCT+TGG	0.505546	4.2:-6680375	None:intergenic
TCAATATCCTTTCATCTCCA+TGG	0.506697	4.2:+6680099	MS.gene72160:CDS
TACAACACTTAACGTGGGTC+GGG	0.508105	4.2:+6680663	MS.gene72160:CDS
AATTGGATGCCCAAACTTGA+CGG	0.510297	4.2:-6680039	None:intergenic
ACCTGGAAATTCAGTATCCA+TGG	0.514112	4.2:-6680116	None:intergenic
AGGTTCTGCAACGCTTTGTA+CGG	0.515634	4.2:+6680622	MS.gene72160:CDS
TTCAAGCGCAATTTATCTCA+TGG	0.520248	4.2:+6680388	MS.gene72160:CDS
CAGATAGGGTGATACCAACT+TGG	0.523809	4.2:-6680231	None:intergenic
GATATTGATTGACAACTTGA+CGG	0.523978	4.2:-6680084	None:intergenic
GCATGCATTTAAGAAAATGA+TGG	0.528476	4.2:+6680732	MS.gene72160:CDS
CTACAACACTTAACGTGGGT+CGG	0.533742	4.2:+6680662	MS.gene72160:CDS
CGGCTATTTGTCCCAAAATG+AGG	0.540450	4.2:-6680271	None:intergenic
GCTGGAAAGTCTCATCAAGC+TGG	0.542113	4.2:+6680688	MS.gene72160:CDS
ATTGACATGCTCTGCCGCCA+AGG	0.544877	4.2:+6680358	MS.gene72160:CDS
AGAGCATGTCAATTGAGCCT+TGG	0.548111	4.2:-6680348	None:intergenic
TTGACATGCTCTGCCGCCAA+GGG	0.557345	4.2:+6680359	MS.gene72160:CDS
GCTTGAAGTCAATCCCTTGG+CGG	0.562753	4.2:-6680372	None:intergenic
GCTCTTAAACTCATCCAAGT+TGG	0.568904	4.2:+6680217	MS.gene72160:CDS
ATGATATGAAACACATGATG+AGG	0.569362	4.2:+6680602	MS.gene72160:CDS
TTGGGTGAATAAATTACACC+TGG	0.570783	4.2:-6680133	None:intergenic
GTTGCCATCACCATCAGATA+GGG	0.583084	4.2:-6680245	None:intergenic
TTCTGCAACGCTTTGTACGG+TGG	0.584961	4.2:+6680625	MS.gene72160:CDS
ATTCAAAATGAACCAGCTGA+AGG	0.587598	4.2:+6680000	None:intergenic
GGTCAGAGGGTTTAAGATGA+CGG	0.592880	4.2:-6680165	None:intergenic
AAGTAACGATAGTGGTCAGA+GGG	0.593594	4.2:-6680178	None:intergenic
TAGTGAAGATATTGACCCGA+AGG	0.595020	4.2:+6680515	MS.gene72160:CDS
TCAGTATCCATGGAGATGAA+AGG	0.598752	4.2:-6680106	None:intergenic
GTTGGTATCACCCTATCTGA+TGG	0.609828	4.2:+6680235	MS.gene72160:CDS
TGACCTTCAAGTAACGATAG+TGG	0.622378	4.2:-6680186	None:intergenic
TATTTGTCCCAAAATGAGGA+AGG	0.623706	4.2:-6680267	None:intergenic
AGTCGCTACAACACTTAACG+TGG	0.625698	4.2:+6680657	MS.gene72160:CDS
CAAGTAACGATAGTGGTCAG+AGG	0.631701	4.2:-6680179	None:intergenic
GAGCATGACCTTTACAACCA+AGG	0.646186	4.2:+6680331	MS.gene72160:CDS
AGTTGAATTCCCATATGTAG+CGG	0.649610	4.2:-6680291	None:intergenic
GATCAGATAGTTTGTTGACG+TGG	0.660381	4.2:+6680710	MS.gene72160:CDS
ATCACCCTATCTGATGGTGA+TGG	0.683961	4.2:+6680241	MS.gene72160:CDS
GTCGCTACAACACTTAACGT+GGG	0.701411	4.2:+6680658	MS.gene72160:CDS
CAGAGGGTTTAAGATGACGG+CGG	0.736962	4.2:-6680162	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATTTAAAAAGTCCTCTAAA+CGG	-	chr4.2:6680552-6680571	None:intergenic	20.0%
!	ATCAAATTCAACCTCTAAAT+TGG	-	chr4.2:6680059-6680078	None:intergenic	25.0%
!	TACTCATTTCTTGAATAATG+AGG	+	chr4.2:6680756-6680775	MS.gene72160:CDS	25.0%
	ATGATATGAAACACATGATG+AGG	+	chr4.2:6680602-6680621	MS.gene72160:CDS	30.0%
	CTTCAACAAATCAGTTGTTT+GGG	+	chr4.2:6680459-6680478	MS.gene72160:CDS	30.0%
	GCATGCATTTAAGAAAATGA+TGG	+	chr4.2:6680732-6680751	MS.gene72160:CDS	30.0%
	TCTTCAACAAATCAGTTGTT+TGG	+	chr4.2:6680458-6680477	MS.gene72160:CDS	30.0%
!	GATATTGATTGACAACTTGA+CGG	-	chr4.2:6680087-6680106	None:intergenic	30.0%
	AAAAAGTCCTCTAAACGGTT+CGG	-	chr4.2:6680547-6680566	None:intergenic	35.0%
	AATCATCATCCGTCAAGTTT+GGG	+	chr4.2:6680030-6680049	MS.gene72160:CDS	35.0%
	AGTTGAATTCCCATATGTAG+CGG	-	chr4.2:6680294-6680313	None:intergenic	35.0%
	TATTTGTCCCAAAATGAGGA+AGG	-	chr4.2:6680270-6680289	None:intergenic	35.0%
	TCAATATCCTTTCATCTCCA+TGG	+	chr4.2:6680099-6680118	MS.gene72160:CDS	35.0%
	TTCAAGCGCAATTTATCTCA+TGG	+	chr4.2:6680388-6680407	MS.gene72160:CDS	35.0%
	TTGGGTGAATAAATTACACC+TGG	-	chr4.2:6680136-6680155	None:intergenic	35.0%
!	TTCACAGCGCTTATGATTTT+GGG	+	chr4.2:6680488-6680507	MS.gene72160:CDS	35.0%
!	TTTCACAGCGCTTATGATTT+TGG	+	chr4.2:6680487-6680506	MS.gene72160:CDS	35.0%
	AATTGGATGCCCAAACTTGA+CGG	-	chr4.2:6680042-6680061	None:intergenic	40.0%
	ACCTGGAAATTCAGTATCCA+TGG	-	chr4.2:6680119-6680138	None:intergenic	40.0%
	AGTTTGGGCATCCAATTTAG+AGG	+	chr4.2:6680045-6680064	MS.gene72160:CDS	40.0%
	CAATCATCATCCGTCAAGTT+TGG	+	chr4.2:6680029-6680048	MS.gene72160:CDS	40.0%
	CAATTGAGCCTTGGTTGTAA+AGG	-	chr4.2:6680342-6680361	None:intergenic	40.0%
	GACAAATAGCCGCTACATAT+GGG	+	chr4.2:6680282-6680301	MS.gene72160:CDS	40.0%
	GCTCTTAAACTCATCCAAGT+TGG	+	chr4.2:6680217-6680236	MS.gene72160:CDS	40.0%
	TAGTGAAGATATTGACCCGA+AGG	+	chr4.2:6680515-6680534	MS.gene72160:CDS	40.0%
	TCAGTATCCATGGAGATGAA+AGG	-	chr4.2:6680109-6680128	None:intergenic	40.0%
	TCCATGGATACTGAATTTCC+AGG	+	chr4.2:6680115-6680134	MS.gene72160:CDS	40.0%
	TGACCACTATCGTTACTTGA+AGG	+	chr4.2:6680183-6680202	MS.gene72160:CDS	40.0%
	TGACCTTCAAGTAACGATAG+TGG	-	chr4.2:6680189-6680208	None:intergenic	40.0%
!	AAGTAACGATAGTGGTCAGA+GGG	-	chr4.2:6680181-6680200	None:intergenic	40.0%
!	ATGGCAACCTTCCTCATTTT+GGG	+	chr4.2:6680260-6680279	MS.gene72160:CDS	40.0%
!	CAAACTTGACGGATGATGAT+TGG	-	chr4.2:6680031-6680050	None:intergenic	40.0%
!!	GATCAGATAGTTTGTTGACG+TGG	+	chr4.2:6680710-6680729	MS.gene72160:CDS	40.0%
	AATGAGGCTCAAAAACACGC+TGG	+	chr4.2:6680772-6680791	MS.gene72160:CDS	45.0%
	AGAGCATGTCAATTGAGCCT+TGG	-	chr4.2:6680351-6680370	None:intergenic	45.0%
	AGGTTCTGCAACGCTTTGTA+CGG	+	chr4.2:6680622-6680641	MS.gene72160:CDS	45.0%
	AGTCGCTACAACACTTAACG+TGG	+	chr4.2:6680657-6680676	MS.gene72160:CDS	45.0%
	ATGAGGCTCAAAAACACGCT+GGG	+	chr4.2:6680773-6680792	MS.gene72160:CDS	45.0%
	CAGATAGGGTGATACCAACT+TGG	-	chr4.2:6680234-6680253	None:intergenic	45.0%
	CGGCTATTTGTCCCAAAATG+AGG	-	chr4.2:6680274-6680293	None:intergenic	45.0%
	CTACAACACTTAACGTGGGT+CGG	+	chr4.2:6680662-6680681	MS.gene72160:CDS	45.0%
	GAGCATGACCTTTACAACCA+AGG	+	chr4.2:6680331-6680350	MS.gene72160:CDS	45.0%
	GCTGAATTGATGCTCACTTC+TGG	+	chr4.2:6680430-6680449	MS.gene72160:CDS	45.0%
	GGACAAATAGCCGCTACATA+TGG	+	chr4.2:6680281-6680300	MS.gene72160:CDS	45.0%
	GTCGCTACAACACTTAACGT+GGG	+	chr4.2:6680658-6680677	MS.gene72160:CDS	45.0%
	GTTGCCATCACCATCAGATA+GGG	-	chr4.2:6680248-6680267	None:intergenic	45.0%
	GTTGGTATCACCCTATCTGA+TGG	+	chr4.2:6680235-6680254	MS.gene72160:CDS	45.0%
	TACAACACTTAACGTGGGTC+GGG	+	chr4.2:6680663-6680682	MS.gene72160:CDS	45.0%
!	AAACACGCTGGGGTACTATT+TGG	+	chr4.2:6680784-6680803	MS.gene72160:CDS	45.0%
!	ATCACCCTATCTGATGGTGA+TGG	+	chr4.2:6680241-6680260	MS.gene72160:CDS	45.0%
!	CAAGTAACGATAGTGGTCAG+AGG	-	chr4.2:6680182-6680201	None:intergenic	45.0%
!	GATGGCAACCTTCCTCATTT+TGG	+	chr4.2:6680259-6680278	MS.gene72160:CDS	45.0%
!	GGTCAGAGGGTTTAAGATGA+CGG	-	chr4.2:6680168-6680187	None:intergenic	45.0%
!!!	TGATTGGCTTTTCCTTCAGC+TGG	-	chr4.2:6680015-6680034	None:intergenic	45.0%
!!!	AATATTTTAACAATATTATT+TGG	+	chr4.2:6680568-6680587	MS.gene72160:CDS	5.0%
	GAATCTGCCGAACCGTTTAG+AGG	+	chr4.2:6680537-6680556	MS.gene72160:CDS	50.0%
	GCTGGAAAGTCTCATCAAGC+TGG	+	chr4.2:6680688-6680707	MS.gene72160:CDS	50.0%
	GCTTGAAGTCAATCCCTTGG+CGG	-	chr4.2:6680375-6680394	None:intergenic	50.0%
	GGTTGCCATCACCATCAGAT+AGG	-	chr4.2:6680249-6680268	None:intergenic	50.0%
	TGAGGCTCAAAAACACGCTG+GGG	+	chr4.2:6680774-6680793	MS.gene72160:CDS	50.0%
	TGCGCTTGAAGTCAATCCCT+TGG	-	chr4.2:6680378-6680397	None:intergenic	50.0%
	TTCTGCAACGCTTTGTACGG+TGG	+	chr4.2:6680625-6680644	MS.gene72160:CDS	50.0%
!	AACGGTTCGGCAGATTCCTT+CGG	-	chr4.2:6680534-6680553	None:intergenic	50.0%
!	AAGATGACGGCGGTCAACTT+TGG	-	chr4.2:6680155-6680174	None:intergenic	50.0%
!	AGATGACGGCGGTCAACTTT+GGG	-	chr4.2:6680154-6680173	None:intergenic	50.0%
!	CAGAGGGTTTAAGATGACGG+CGG	-	chr4.2:6680165-6680184	None:intergenic	50.0%
	ATTGACATGCTCTGCCGCCA+AGG	+	chr4.2:6680358-6680377	MS.gene72160:CDS	55.0%
	TTGACATGCTCTGCCGCCAA+GGG	+	chr4.2:6680359-6680378	MS.gene72160:CDS	55.0%
!	ACGGTTCGGCAGATTCCTTC+GGG	-	chr4.2:6680533-6680552	None:intergenic	55.0%
!	CTTAACGTGGGTCGGGTTGC+TGG	+	chr4.2:6680670-6680689	MS.gene72160:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	6680007	6680825	6680007	ID=MS.gene72160
chr4.2	mRNA	6680007	6680825	6680007	ID=MS.gene72160.t1;Parent=MS.gene72160
chr4.2	exon	6680007	6680825	6680007	ID=MS.gene72160.t1.exon1;Parent=MS.gene72160.t1
chr4.2	CDS	6680007	6680825	6680007	ID=cds.MS.gene72160.t1;Parent=MS.gene72160.t1

Gene Sequence

>MS.gene72160

ATGAACCAGCTGAAGGAAAAGCCAATCATCATCCGTCAAGTTTGGGCATCCAATTTAGAGGTTGAATTTGATCTCATCCGTCAAGTTGTCAATCAATATCCTTTCATCTCCATGGATACTGAATTTCCAGGTGTAATTTATTCACCCAAAGTTGACCGCCGTCATCTTAAACCCTCTGACCACTATCGTTACTTGAAGGTCAACGTTGATGCTCTTAAACTCATCCAAGTTGGTATCACCCTATCTGATGGTGATGGCAACCTTCCTCATTTTGGGACAAATAGCCGCTACATATGGGAATTCAACTTTTGCGATTTTGATTTTGAGCATGACCTTTACAACCAAGGCTCAATTGACATGCTCTGCCGCCAAGGGATTGACTTCAAGCGCAATTTATCTCATGGTGTGAATTCGTCACGTTTTGCTGAATTGATGCTCACTTCTGGACTTGTCTTCAACAAATCAGTTGTTTGGGTCACATTTCACAGCGCTTATGATTTTGGGTATTTAGTGAAGATATTGACCCGAAGGAATCTGCCGAACCGTTTAGAGGACTTTTTAAATATTTTAACAATATTATTTGGAAAGAATGTTTATGATATGAAACACATGATGAGGTTCTGCAACGCTTTGTACGGTGGTCTCGAGCGAGTCGCTACAACACTTAACGTGGGTCGGGTTGCTGGAAAGTCTCATCAAGCTGGATCAGATAGTTTGTTGACGTGGCATGCATTTAAGAAAATGATGGATACTCATTTCTTGAATAATGAGGCTCAAAAACACGCTGGGGTACTATTTGGATTAGAAATTGCAACTTAG

Protein sequence

>MS.gene72160.t1

MNQLKEKPIIIRQVWASNLEVEFDLIRQVVNQYPFISMDTEFPGVIYSPKVDRRHLKPSDHYRYLKVNVDALKLIQVGITLSDGDGNLPHFGTNSRYIWEFNFCDFDFEHDLYNQGSIDMLCRQGIDFKRNLSHGVNSSRFAELMLTSGLVFNKSVVWVTFHSAYDFGYLVKILTRRNLPNRLEDFLNILTILFGKNVYDMKHMMRFCNALYGGLERVATTLNVGRVAGKSHQAGSDSLLTWHAFKKMMDTHFLNNEAQKHAGVLFGLEIAT