AlfalfaGEDB | MS.gene73870

M. sativa cultivar XinJiangDaYe / MS.gene73870

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73870.t1	KEH21095.1	97.3	264	7	0	1	264	83	346	8.40E-128	466.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73870.t1	Q9LDD4	51.7	207	93	3	59	263	372	573	6.7e-57	222.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73870.t1	A0A072U5F8	97.3	264	7	0	1	264	83	346	6.1e-128	466.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049368	MS.gene73870	0.800349	1.50E-48	-1.69E-46
MS.gene049786	MS.gene73870	0.805311	1.42E-49	-1.69E-46
MS.gene051363	MS.gene73870	0.816883	4.40E-52	-1.69E-46
MS.gene051365	MS.gene73870	0.808306	3.31E-50	-1.69E-46
MS.gene051700	MS.gene73870	0.804191	2.43E-49	-1.69E-46
MS.gene05188	MS.gene73870	0.840476	8.50E-58	-1.69E-46
MS.gene051902	MS.gene73870	0.815959	7.07E-52	-1.69E-46
MS.gene051975	MS.gene73870	0.827074	1.91E-54	-1.69E-46
MS.gene052108	MS.gene73870	0.800567	1.36E-48	-1.69E-46
MS.gene053226	MS.gene73870	0.803326	3.68E-49	-1.69E-46
MS.gene053298	MS.gene73870	0.803743	3.01E-49	-1.69E-46
MS.gene054479	MS.gene73870	0.813257	2.81E-51	-1.69E-46
MS.gene05458	MS.gene73870	0.80545	1.33E-49	-1.69E-46
MS.gene05469	MS.gene73870	0.802651	5.07E-49	-1.69E-46
MS.gene05517	MS.gene73870	0.816985	4.17E-52	-1.69E-46
MS.gene055313	MS.gene73870	0.804505	2.09E-49	-1.69E-46
MS.gene055513	MS.gene73870	0.802532	5.36E-49	-1.69E-46
MS.gene055603	MS.gene73870	0.829991	3.78E-55	-1.69E-46
MS.gene056403	MS.gene73870	0.809532	1.81E-50	-1.69E-46
MS.gene056509	MS.gene73870	0.8014	9.17E-49	-1.69E-46
MS.gene056986	MS.gene73870	0.836422	9.45E-57	-1.69E-46
MS.gene057102	MS.gene73870	0.806058	9.89E-50	-1.69E-46
MS.gene057208	MS.gene73870	0.802229	6.19E-49	-1.69E-46
MS.gene06011	MS.gene73870	0.830948	2.20E-55	-1.69E-46
MS.gene060365	MS.gene73870	0.829426	5.18E-55	-1.69E-46
MS.gene060763	MS.gene73870	0.80408	2.56E-49	-1.69E-46
MS.gene060935	MS.gene73870	0.809972	1.45E-50	-1.69E-46
MS.gene060937	MS.gene73870	0.818016	2.44E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73870.t1	MTR_8g098645	97.348	264	7	0	1	264	83	346	0.0	541
MS.gene73870.t1	MTR_8g098605	78.030	264	33	2	1	264	358	596	1.29e-144	418
MS.gene73870.t1	MTR_1g073380	75.581	258	62	1	1	258	336	592	4.66e-138	401
MS.gene73870.t1	MTR_1g073590	73.643	258	65	1	1	258	325	579	2.31e-131	384
MS.gene73870.t1	MTR_1g073530	73.643	258	65	1	1	258	328	582	2.94e-131	384
MS.gene73870.t1	MTR_8g098635	85.427	199	29	0	66	264	99	297	3.32e-125	358
MS.gene73870.t1	MTR_8g098635	85.427	199	29	0	66	264	182	380	1.25e-123	357
MS.gene73870.t1	MTR_0259s0060	58.011	181	29	1	59	239	14	147	6.97e-65	200
MS.gene73870.t1	MTR_7g078120	51.892	185	89	0	61	245	387	571	4.59e-61	202
MS.gene73870.t1	MTR_7g078120	51.892	185	89	0	61	245	387	571	1.13e-60	202
MS.gene73870.t1	MTR_8g028905	52.000	175	80	3	61	231	408	582	1.13e-53	183
MS.gene73870.t1	MTR_6g071350	75.490	102	25	0	62	163	273	374	2.55e-47	162
MS.gene73870.t1	MTR_6g071350	73.171	41	11	0	224	264	361	401	6.00e-13	68.6
MS.gene73870.t1	MTR_1g073420	79.710	69	14	0	190	258	60	128	9.68e-35	122
MS.gene73870.t1	MTR_6g071355	62.105	95	17	1	152	246	1	76	8.32e-32	112
MS.gene73870.t1	MTR_7g066890	38.012	171	98	2	60	224	193	361	1.59e-30	117
MS.gene73870.t1	MTR_5g048010	55.285	123	39	2	42	163	182	289	1.96e-30	115
MS.gene73870.t1	MTR_5g048010	75.610	41	10	0	224	264	276	316	1.70e-13	69.3
MS.gene73870.t1	MTR_7g066890	38.012	171	98	2	60	224	295	463	7.77e-30	117
MS.gene73870.t1	MTR_3g023780	39.891	183	95	6	59	229	147	326	4.24e-28	112
MS.gene73870.t1	MTR_7g066880	30.387	181	118	2	50	224	175	353	2.17e-21	92.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73870.t1	AT4G11400	47.881	236	111	4	30	263	348	573	2.29e-67	219
MS.gene73870.t1	AT4G11400	47.881	236	111	4	30	263	348	573	2.29e-67	219
MS.gene73870.t1	AT2G46040	46.199	171	88	3	63	230	362	531	9.29e-43	153
MS.gene73870.t1	AT2G46040	46.199	171	88	3	63	230	362	531	9.29e-43	153
MS.gene73870.t1	AT2G46040	46.199	171	88	3	63	230	376	545	1.14e-42	153
MS.gene73870.t1	AT5G04110	39.130	161	86	4	61	210	362	521	6.91e-29	115
MS.gene73870.t1	AT5G04110	39.130	161	86	4	61	210	361	520	7.58e-29	114
MS.gene73870.t1	AT5G04110	39.130	161	86	4	61	210	361	520	7.58e-29	114
MS.gene73870.t1	AT2G03470	34.896	192	102	6	59	235	120	303	2.22e-23	99.0
MS.gene73870.t1	AT2G03470	34.896	192	102	6	59	235	121	304	2.44e-23	98.6
MS.gene73870.t1	AT1G13880	35.359	181	103	4	59	229	127	303	3.11e-23	98.2
MS.gene73870.t1	AT1G13880	35.359	181	103	4	59	229	126	302	3.37e-23	98.2
MS.gene73870.t1	AT1G13880	35.359	181	103	4	59	229	136	312	4.04e-23	97.8
MS.gene73870.t1	AT1G26580	31.707	205	120	5	59	245	67	269	1.30e-22	96.3
MS.gene73870.t1	AT1G26580	31.707	205	120	5	59	245	133	335	1.65e-22	96.7
MS.gene73870.t1	AT1G26580	31.707	205	120	5	59	245	133	335	1.65e-22	96.7
MS.gene73870.t1	AT5G04110	37.241	145	79	4	61	194	361	504	1.87e-20	90.9

Find 40 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCATTGGGGATTTGATAAAA+TGG	0.191990	4.1:+5751652	MS.gene73870:CDS
GATGATGACGAAGTTGAATT+TGG	0.250331	4.1:+5751893	MS.gene73870:CDS
TGATGAAGAAAAGAGATTTA+AGG	0.278264	4.1:+5751703	MS.gene73870:CDS
TGCCTTGTTTCGAAAGTATC+TGG	0.279788	4.1:-5751780	None:intergenic
CACTCCAAGCAATCCATATA+TGG	0.312317	4.1:-5751956	None:intergenic
ACAAGATCTGTTTCGGTAGT+AGG	0.317915	4.1:-5751512	None:intergenic
TTTCTCTTCTCAGTTGCATC+TGG	0.327601	4.1:-5751299	None:intergenic
CTTCTCAGTTGCATCTGGTT+TGG	0.334667	4.1:-5751294	None:intergenic
GAGAAAATCTGAGCCATCTA+TGG	0.335639	4.1:+5751352	MS.gene73870:CDS
TTTCTGAGAGTGACTCTAAA+TGG	0.355739	4.1:+5751450	MS.gene73870:CDS
TTCAGTGACGACGTCTGGTT+TGG	0.357587	4.1:-5751255	None:intergenic
AGCTATCAAGCATCCATATA+TGG	0.363115	4.1:+5751943	MS.gene73870:CDS
AATGGTTAGGCACACAAGTA+TGG	0.373689	4.1:+5751468	MS.gene73870:CDS
GAAGTTGAATTTGGATCTTT+TGG	0.375334	4.1:+5751902	MS.gene73870:CDS
CATTGGGGATTTGATAAAAT+GGG	0.380212	4.1:+5751653	MS.gene73870:CDS
CGAAAATCATCATCCATAGA+TGG	0.407291	4.1:-5751365	None:intergenic
TCTCCCAACAAGATCTGTTT+CGG	0.428135	4.1:-5751519	None:intergenic
GATTTCATATTGCTGAGAAC+AGG	0.435888	4.1:+5751594	MS.gene73870:CDS
CCGGAACTTCCGCTTGGAAA+AGG	0.440137	4.1:-5751412	None:intergenic
GAAACTACACCAGTCCATTC+CGG	0.442162	4.1:-5751431	None:intergenic
CTACCGAAACAGATCTTGTT+GGG	0.462379	4.1:+5751516	MS.gene73870:CDS
GCATCCATATATGGATTGCT+TGG	0.469079	4.1:+5751952	MS.gene73870:CDS
CGTGAAAAGCTTGTTTCTGT+AGG	0.477267	4.1:+5751386	MS.gene73870:CDS
TCATCGCTGTCAACAGTCTT+CGG	0.477687	4.1:-5751875	None:intergenic
GAGAGTGACTCTAAATGGTT+AGG	0.492462	4.1:+5751455	MS.gene73870:CDS
AAGTGTAGCTGCAACGTTCA+AGG	0.494639	4.1:+5751554	MS.gene73870:CDS
GGAGTCAGAAATATGTCCTC+TGG	0.498373	4.1:+5751220	MS.gene73870:CDS
ACTACCGAAACAGATCTTGT+TGG	0.499677	4.1:+5751515	MS.gene73870:CDS
TCCATTCCGGAACTTCCGCT+TGG	0.513689	4.1:-5751418	None:intergenic
GTGAGGAAGTTTCACTTCGA+TGG	0.516591	4.1:+5751675	MS.gene73870:CDS
TTGTTTCGAAAGTATCTGGA+TGG	0.538515	4.1:-5751776	None:intergenic
GCGGAAGTTCCGGAATGGAC+TGG	0.543878	4.1:+5751422	MS.gene73870:CDS
GGGATTTGATAAAATGGGTG+AGG	0.544818	4.1:+5751658	MS.gene73870:CDS
TCCAAGCGGAAGTTCCGGAA+TGG	0.566498	4.1:+5751417	MS.gene73870:CDS
ATCCAGATACTTTCGAAACA+AGG	0.591847	4.1:+5751778	MS.gene73870:CDS
GAAACAGATCTTGTTGGGAG+AGG	0.634208	4.1:+5751521	MS.gene73870:CDS
CTTGTTGGGAGAGGAAGACG+AGG	0.636192	4.1:+5751530	MS.gene73870:CDS
AGAGATTTAAGGATGCAATG+AGG	0.659010	4.1:+5751714	MS.gene73870:CDS
AAACAAGGCAAGAAAAGACA+TGG	0.666046	4.1:+5751793	MS.gene73870:CDS
AAATCCCCAATGATAAAACA+CGG	0.674548	4.1:-5751642	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGATGAAGAAAAGAGATTTA+AGG	+	chr4.1:5751703-5751722	MS.gene73870:CDS	25.0%
!!	TTCTCTTTTCAGTAATATCT+GGG	-	chr4.1:5751337-5751356	None:intergenic	25.0%
!!	TTTCTCTTTTCAGTAATATC+TGG	-	chr4.1:5751338-5751357	None:intergenic	25.0%
	AAATCCCCAATGATAAAACA+CGG	-	chr4.1:5751645-5751664	None:intergenic	30.0%
	CATTGGGGATTTGATAAAAT+GGG	+	chr4.1:5751653-5751672	MS.gene73870:CDS	30.0%
	TCATTGGGGATTTGATAAAA+TGG	+	chr4.1:5751652-5751671	MS.gene73870:CDS	30.0%
!	GAAGTTGAATTTGGATCTTT+TGG	+	chr4.1:5751902-5751921	MS.gene73870:CDS	30.0%
!!	GTTCCAAAAAGACTTGTTTT+GGG	-	chr4.1:5751753-5751772	None:intergenic	30.0%
!!	TGTTCCAAAAAGACTTGTTT+TGG	-	chr4.1:5751754-5751773	None:intergenic	30.0%
!!!	GGTTTTGAATCATCTTTAAC+AGG	-	chr4.1:5751494-5751513	None:intergenic	30.0%
	AAACAAGGCAAGAAAAGACA+TGG	+	chr4.1:5751793-5751812	MS.gene73870:CDS	35.0%
	AGAGATTTAAGGATGCAATG+AGG	+	chr4.1:5751714-5751733	MS.gene73870:CDS	35.0%
	AGCTATCAAGCATCCATATA+TGG	+	chr4.1:5751943-5751962	MS.gene73870:CDS	35.0%
	ATCCAGATACTTTCGAAACA+AGG	+	chr4.1:5751778-5751797	MS.gene73870:CDS	35.0%
	CGAAAATCATCATCCATAGA+TGG	-	chr4.1:5751368-5751387	None:intergenic	35.0%
	GATTTCATATTGCTGAGAAC+AGG	+	chr4.1:5751594-5751613	MS.gene73870:CDS	35.0%
!	CATTCCGTGTTTTATCATTG+GGG	+	chr4.1:5751638-5751657	MS.gene73870:CDS	35.0%
!	CTTTTCAGTAATATCTGGGT+TGG	-	chr4.1:5751333-5751352	None:intergenic	35.0%
!	GAATTTGGATCTTTTGGTGA+TGG	+	chr4.1:5751908-5751927	MS.gene73870:CDS	35.0%
!	GATGATGACGAAGTTGAATT+TGG	+	chr4.1:5751893-5751912	MS.gene73870:CDS	35.0%
!	GCATTCCGTGTTTTATCATT+GGG	+	chr4.1:5751637-5751656	MS.gene73870:CDS	35.0%
!	TGCATTCCGTGTTTTATCAT+TGG	+	chr4.1:5751636-5751655	MS.gene73870:CDS	35.0%
!	TTTCTGAGAGTGACTCTAAA+TGG	+	chr4.1:5751450-5751469	MS.gene73870:CDS	35.0%
!!	TTGTTTCGAAAGTATCTGGA+TGG	-	chr4.1:5751779-5751798	None:intergenic	35.0%
!!!	AAAAAGACTTGTTTTGGGAG+GGG	-	chr4.1:5751748-5751767	None:intergenic	35.0%
!!!	CAAAAAGACTTGTTTTGGGA+GGG	-	chr4.1:5751749-5751768	None:intergenic	35.0%
!!!	GATCTTTTGGTGATGGTTTT+GGG	+	chr4.1:5751915-5751934	MS.gene73870:CDS	35.0%
	AATGGTTAGGCACACAAGTA+TGG	+	chr4.1:5751468-5751487	MS.gene73870:CDS	40.0%
	ACAAGATCTGTTTCGGTAGT+AGG	-	chr4.1:5751515-5751534	None:intergenic	40.0%
	ACTACCGAAACAGATCTTGT+TGG	+	chr4.1:5751515-5751534	MS.gene73870:CDS	40.0%
	CACTCCAAGCAATCCATATA+TGG	-	chr4.1:5751959-5751978	None:intergenic	40.0%
	CTACCGAAACAGATCTTGTT+GGG	+	chr4.1:5751516-5751535	MS.gene73870:CDS	40.0%
	GAGAAAATCTGAGCCATCTA+TGG	+	chr4.1:5751352-5751371	MS.gene73870:CDS	40.0%
	GAGAGTGACTCTAAATGGTT+AGG	+	chr4.1:5751455-5751474	MS.gene73870:CDS	40.0%
	GGGATTTGATAAAATGGGTG+AGG	+	chr4.1:5751658-5751677	MS.gene73870:CDS	40.0%
	TCTCCCAACAAGATCTGTTT+CGG	-	chr4.1:5751522-5751541	None:intergenic	40.0%
	TTTCTCTTCTCAGTTGCATC+TGG	-	chr4.1:5751302-5751321	None:intergenic	40.0%
!	CCTCCCAAAACAAGTCTTTT+TGG	+	chr4.1:5751747-5751766	MS.gene73870:CDS	40.0%
!	GCATCCATATATGGATTGCT+TGG	+	chr4.1:5751952-5751971	MS.gene73870:CDS	40.0%
!!	CGTGAAAAGCTTGTTTCTGT+AGG	+	chr4.1:5751386-5751405	MS.gene73870:CDS	40.0%
!!	TGCCTTGTTTCGAAAGTATC+TGG	-	chr4.1:5751783-5751802	None:intergenic	40.0%
!!!	CCAAAAAGACTTGTTTTGGG+AGG	-	chr4.1:5751750-5751769	None:intergenic	40.0%
!!!	GGATCTTTTGGTGATGGTTT+TGG	+	chr4.1:5751914-5751933	MS.gene73870:CDS	40.0%
!!!	TTTTGGTGATGGTTTTGGGA+GGG	+	chr4.1:5751919-5751938	MS.gene73870:CDS	40.0%
	AAGTGTAGCTGCAACGTTCA+AGG	+	chr4.1:5751554-5751573	MS.gene73870:CDS	45.0%
	CTTCTCAGTTGCATCTGGTT+TGG	-	chr4.1:5751297-5751316	None:intergenic	45.0%
	GAAACAGATCTTGTTGGGAG+AGG	+	chr4.1:5751521-5751540	MS.gene73870:CDS	45.0%
	GAAACTACACCAGTCCATTC+CGG	-	chr4.1:5751434-5751453	None:intergenic	45.0%
	GGAGTCAGAAATATGTCCTC+TGG	+	chr4.1:5751220-5751239	MS.gene73870:CDS	45.0%
	GTGAGGAAGTTTCACTTCGA+TGG	+	chr4.1:5751675-5751694	MS.gene73870:CDS	45.0%
	TCATCGCTGTCAACAGTCTT+CGG	-	chr4.1:5751878-5751897	None:intergenic	45.0%
!	TGTAGGTCACCTTTTCCAAG+CGG	+	chr4.1:5751403-5751422	MS.gene73870:CDS	45.0%
!	TTCTTTTCAGTGACGACGTC+TGG	-	chr4.1:5751263-5751282	None:intergenic	45.0%
!!!	CTTTTGGTGATGGTTTTGGG+AGG	+	chr4.1:5751918-5751937	MS.gene73870:CDS	45.0%
!!!	TTGGCTGTTGTTTTCTCCAG+AGG	-	chr4.1:5751239-5751258	None:intergenic	45.0%
	TTCAGTGACGACGTCTGGTT+TGG	-	chr4.1:5751258-5751277	None:intergenic	50.0%
!	CCTTTTCCAAGCGGAAGTTC+CGG	+	chr4.1:5751412-5751431	MS.gene73870:CDS	50.0%
	CCGGAACTTCCGCTTGGAAA+AGG	-	chr4.1:5751415-5751434	None:intergenic	55.0%
	CTTGTTGGGAGAGGAAGACG+AGG	+	chr4.1:5751530-5751549	MS.gene73870:CDS	55.0%
	TCCAAGCGGAAGTTCCGGAA+TGG	+	chr4.1:5751417-5751436	MS.gene73870:CDS	55.0%
	TCCATTCCGGAACTTCCGCT+TGG	-	chr4.1:5751421-5751440	None:intergenic	55.0%
	GCGGAAGTTCCGGAATGGAC+TGG	+	chr4.1:5751422-5751441	MS.gene73870:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	5751218	5752012	5751218	ID=MS.gene73870
chr4.1	mRNA	5751218	5752012	5751218	ID=MS.gene73870.t1;Parent=MS.gene73870
chr4.1	exon	5751218	5752012	5751218	ID=MS.gene73870.t1.exon1;Parent=MS.gene73870.t1
chr4.1	CDS	5751218	5752012	5751218	ID=cds.MS.gene73870.t1;Parent=MS.gene73870.t1

Gene Sequence

>MS.gene73870

ATGGAGTCAGAAATATGTCCTCTGGAGAAAACAACAGCCAAACCAGACGTCGTCACTGAAAAGAAAAAATCAACTGCCAAACCAGATGCAACTGAGAAGAGAAAATCAACTGCCAACCCAGATATTACTGAAAAGAGAAAATCTGAGCCATCTATGGATGATGATTTTCGTGAAAAGCTTGTTTCTGTAGGTCACCTTTTCCAAGCGGAAGTTCCGGAATGGACTGGTGTAGTTTCTGAGAGTGACTCTAAATGGTTAGGCACACAAGTATGGCCTGTTAAAGATGATTCAAAACCTACTACCGAAACAGATCTTGTTGGGAGAGGAAGACGAGGAAAGTGTAGCTGCAACGTTCAAGGTTCTGTTGACTGTGTCAGATTTCATATTGCTGAGAACAGGATGAAGCTGAAGCTTGAATTGCATTCCGTGTTTTATCATTGGGGATTTGATAAAATGGGTGAGGAAGTTTCACTTCGATGGACAGCTGATGAAGAAAAGAGATTTAAGGATGCAATGAGGTTAAAATTCCCCTCCCAAAACAAGTCTTTTTGGAACAATCCATCCAGATACTTTCGAAACAAGGCAAGAAAAGACATGGTGAGCTATTACTTCAATGTATATCTTATTCAACTAAGAAGCTATCAGAATCGAGTGACTCCGAAGACTGTTGACAGCGATGATGACGAAGTTGAATTTGGATCTTTTGGTGATGGTTTTGGGAGGGAAGCTATCAAGCATCCATATATGGATTGCTTGGAGTGTTCAGAGAACAAGCAATGCTTTGACTTTGAGTAG

Protein sequence

>MS.gene73870.t1

MESEICPLEKTTAKPDVVTEKKKSTAKPDATEKRKSTANPDITEKRKSEPSMDDDFREKLVSVGHLFQAEVPEWTGVVSESDSKWLGTQVWPVKDDSKPTTETDLVGRGRRGKCSCNVQGSVDCVRFHIAENRMKLKLELHSVFYHWGFDKMGEEVSLRWTADEEKRFKDAMRLKFPSQNKSFWNNPSRYFRNKARKDMVSYYFNVYLIQLRSYQNRVTPKTVDSDDDEVEFGSFGDGFGREAIKHPYMDCLECSENKQCFDFE