Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75460.t1 | XP_003601984.1 | 99.4 | 160 | 1 | 0 | 1 | 160 | 1 | 160 | 4.80E-86 | 327 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75460.t1 | Q9LX15 | 46.9 | 162 | 81 | 3 | 1 | 159 | 1 | 160 | 8.5e-31 | 134.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75460.t1 | G7J4B2 | 99.4 | 160 | 1 | 0 | 1 | 160 | 1 | 160 | 3.5e-86 | 327.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050329 | MS.gene75460 | 0.81919 | 1.32E-52 | -1.69E-46 |
| MS.gene050426 | MS.gene75460 | 0.804064 | 2.58E-49 | -1.69E-46 |
| MS.gene051158 | MS.gene75460 | 0.818625 | 1.78E-52 | -1.69E-46 |
| MS.gene051552 | MS.gene75460 | 0.807059 | 6.08E-50 | -1.69E-46 |
| MS.gene051996 | MS.gene75460 | 0.844333 | 8.07E-59 | -1.69E-46 |
| MS.gene052011 | MS.gene75460 | 0.805475 | 1.31E-49 | -1.69E-46 |
| MS.gene052223 | MS.gene75460 | 0.965981 | 2.58E-125 | -1.69E-46 |
| MS.gene05233 | MS.gene75460 | 0.815249 | 1.02E-51 | -1.69E-46 |
| MS.gene052705 | MS.gene75460 | 0.810583 | 1.07E-50 | -1.69E-46 |
| MS.gene052706 | MS.gene75460 | 0.806903 | 6.56E-50 | -1.69E-46 |
| MS.gene054138 | MS.gene75460 | 0.800691 | 1.28E-48 | -1.69E-46 |
| MS.gene054277 | MS.gene75460 | 0.840264 | 9.65E-58 | -1.69E-46 |
| MS.gene054742 | MS.gene75460 | 0.850789 | 1.35E-60 | -1.69E-46 |
| MS.gene055601 | MS.gene75460 | 0.811437 | 7.00E-51 | -1.69E-46 |
| MS.gene056330 | MS.gene75460 | 0.870311 | 1.61E-66 | -1.69E-46 |
| MS.gene056680 | MS.gene75460 | 0.837173 | 6.07E-57 | -1.69E-46 |
| MS.gene056690 | MS.gene75460 | 0.851953 | 6.34E-61 | -1.69E-46 |
| MS.gene056691 | MS.gene75460 | 0.840178 | 1.02E-57 | -1.69E-46 |
| MS.gene056899 | MS.gene75460 | 0.808853 | 2.52E-50 | -1.69E-46 |
| MS.gene056920 | MS.gene75460 | 0.846551 | 2.02E-59 | -1.69E-46 |
| MS.gene056923 | MS.gene75460 | 0.84803 | 7.95E-60 | -1.69E-46 |
| MS.gene056924 | MS.gene75460 | 0.84466 | 6.59E-59 | -1.69E-46 |
| MS.gene056925 | MS.gene75460 | 0.829186 | 5.92E-55 | -1.69E-46 |
| MS.gene059043 | MS.gene75460 | 0.802479 | 5.50E-49 | -1.69E-46 |
| MS.gene059328 | MS.gene75460 | 0.801232 | 9.92E-49 | -1.69E-46 |
| MS.gene059891 | MS.gene75460 | 0.807887 | 4.06E-50 | -1.69E-46 |
| MS.gene059892 | MS.gene75460 | 0.802803 | 4.72E-49 | -1.69E-46 |
| MS.gene060087 | MS.gene75460 | 0.832733 | 7.99E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75460.t1 | MTR_3g087540 | 99.375 | 160 | 1 | 0 | 1 | 160 | 1 | 160 | 3.90e-119 | 333 |
| MS.gene75460.t1 | MTR_3g073990 | 39.860 | 143 | 78 | 3 | 16 | 152 | 13 | 153 | 4.66e-31 | 110 |
| MS.gene75460.t1 | MTR_2g016020 | 34.848 | 132 | 78 | 5 | 25 | 152 | 28 | 155 | 1.78e-22 | 88.2 |
| MS.gene75460.t1 | MTR_2g077940 | 27.160 | 162 | 99 | 4 | 4 | 151 | 8 | 164 | 1.74e-13 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75460.t1 | AT5G10130 | 46.914 | 162 | 81 | 3 | 1 | 159 | 1 | 160 | 3.17e-45 | 146 |
| MS.gene75460.t1 | AT4G08685 | 40.994 | 161 | 84 | 4 | 5 | 159 | 4 | 159 | 5.11e-36 | 122 |
| MS.gene75460.t1 | AT1G78040 | 36.095 | 169 | 92 | 5 | 1 | 159 | 1 | 163 | 5.76e-34 | 117 |
| MS.gene75460.t1 | AT1G78040 | 36.095 | 169 | 92 | 5 | 1 | 159 | 1 | 163 | 5.76e-34 | 117 |
| MS.gene75460.t1 | AT1G78040 | 36.095 | 169 | 92 | 5 | 1 | 159 | 1 | 163 | 5.76e-34 | 117 |
| MS.gene75460.t1 | AT1G29140 | 35.669 | 157 | 92 | 3 | 4 | 152 | 6 | 161 | 1.14e-26 | 99.0 |
| MS.gene75460.t1 | AT5G45880 | 39.024 | 123 | 70 | 3 | 25 | 143 | 35 | 156 | 3.91e-23 | 90.1 |
| MS.gene75460.t1 | AT4G18596 | 37.705 | 122 | 71 | 3 | 25 | 142 | 33 | 153 | 2.57e-22 | 87.8 |
| MS.gene75460.t1 | AT4G18596 | 37.705 | 122 | 71 | 3 | 25 | 142 | 79 | 199 | 6.42e-22 | 88.2 |
Find 45 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGATTGTGTTGCTGTTAA+TGG | 0.278593 | 3.3:-72956698 | MS.gene75460:CDS |
| AATAAACACAACCTTTGATA+TGG | 0.316996 | 3.3:+72956629 | None:intergenic |
| GTGTATTACACGATGATATT+GGG | 0.373900 | 3.3:+72956179 | None:intergenic |
| GGTGTATTACACGATGATAT+TGG | 0.406710 | 3.3:+72956178 | None:intergenic |
| CACTGGAACATACAACATAA+TGG | 0.429786 | 3.3:-72956251 | MS.gene75460:CDS |
| CGTCCATAGTCTTCCTGTCT+TGG | 0.431661 | 3.3:+72956305 | None:intergenic |
| ACAAGTAATATTTGAGGAGT+TGG | 0.437901 | 3.3:+72956032 | None:intergenic |
| AAATGCTATGGGTTACTTCA+AGG | 0.456909 | 3.3:-72956080 | MS.gene75460:CDS |
| TAGGTGCAAAGGTGGGAATT+CGG | 0.460306 | 3.3:-72956330 | MS.gene75460:CDS |
| TGGGAATTCGGTGCCAAGAC+AGG | 0.469322 | 3.3:-72956318 | MS.gene75460:CDS |
| TATTGTGATACTTGCCGTGC+TGG | 0.471219 | 3.3:-72956610 | MS.gene75460:CDS |
| GTGCCAAGACAGGAAGACTA+TGG | 0.475430 | 3.3:-72956308 | MS.gene75460:CDS |
| TCTTCACTATGCAAATGCTA+TGG | 0.481660 | 3.3:-72956092 | MS.gene75460:CDS |
| GGAGGATAGAGCTGAGGGAA+AGG | 0.482593 | 3.3:+72956674 | None:intergenic |
| TAGCATTTGCATAGTGAAGA+TGG | 0.484441 | 3.3:+72956095 | None:intergenic |
| GATCTGTTACGCCCAGGGTC+CGG | 0.486304 | 3.3:+72956157 | None:intergenic |
| AACATAGCTGTGGCAAGTAA+AGG | 0.486675 | 3.3:+72956652 | None:intergenic |
| ACATAGCTGTGGCAAGTAAA+GGG | 0.489307 | 3.3:+72956653 | None:intergenic |
| GAGGATAGAGCTGAGGGAAA+GGG | 0.496587 | 3.3:+72956675 | None:intergenic |
| AGGAGTTGGTGGCAATATTG+AGG | 0.508425 | 3.3:+72956046 | None:intergenic |
| AATCAGACAAGTAATATTTG+AGG | 0.510401 | 3.3:+72956026 | None:intergenic |
| TGCTGGATCTGTTACGCCCA+GGG | 0.512778 | 3.3:+72956152 | None:intergenic |
| AGTAATATTTGAGGAGTTGG+TGG | 0.513246 | 3.3:+72956035 | None:intergenic |
| GTAGTGAGTGAGCACAATGC+TGG | 0.521929 | 3.3:+72956135 | None:intergenic |
| ATGCTGGATCTGTTACGCCC+AGG | 0.535067 | 3.3:+72956151 | None:intergenic |
| CTTCACTATGCAAATGCTAT+GGG | 0.535954 | 3.3:-72956091 | MS.gene75460:CDS |
| TCGTGTAATACACCGGACCC+TGG | 0.543647 | 3.3:-72956169 | MS.gene75460:CDS |
| TTGCATAGTGAAGATGGTTG+AGG | 0.549905 | 3.3:+72956101 | None:intergenic |
| TTCTTGATTAGGTGCAAAGG+TGG | 0.550705 | 3.3:-72956338 | MS.gene75460:intron |
| CGTGTAATACACCGGACCCT+GGG | 0.556675 | 3.3:-72956168 | MS.gene75460:CDS |
| TGGTGGCAATATTGAGGCAA+AGG | 0.557338 | 3.3:+72956052 | None:intergenic |
| CATTATGTTGTATGTTCCAG+TGG | 0.561706 | 3.3:+72956252 | None:intergenic |
| CATTGCTAATCATCATGGCA+AGG | 0.587013 | 3.3:-72956717 | None:intergenic |
| CGATTCTTGATTAGGTGCAA+AGG | 0.587702 | 3.3:-72956341 | MS.gene75460:intron |
| ACAGCTATGTTCCATATCAA+AGG | 0.587960 | 3.3:-72956640 | MS.gene75460:CDS |
| TAAAGGGAGGATAGAGCTGA+GGG | 0.597004 | 3.3:+72956669 | None:intergenic |
| GTAAAGGGAGGATAGAGCTG+AGG | 0.612679 | 3.3:+72956668 | None:intergenic |
| GAAGGAGTGACAGATTCCAC+TGG | 0.617675 | 3.3:-72956268 | MS.gene75460:CDS |
| TTGGATGTAAAATGTGGCGT+TGG | 0.634933 | 3.3:+72956579 | None:intergenic |
| CGTTGGTTTCGAATCCAGCA+CGG | 0.637233 | 3.3:+72956596 | None:intergenic |
| TCTTGATTAGGTGCAAAGGT+GGG | 0.642740 | 3.3:-72956337 | MS.gene75460:intron |
| AATATCATCGTGTAATACAC+CGG | 0.667061 | 3.3:-72956176 | MS.gene75460:CDS |
| TTTACCTTGGATGTAAAATG+TGG | 0.673598 | 3.3:+72956573 | None:intergenic |
| TTTGATATGGAACATAGCTG+TGG | 0.697489 | 3.3:+72956642 | None:intergenic |
| TAGCTGTGGCAAGTAAAGGG+AGG | 0.702643 | 3.3:+72956656 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTAAAAAATTTACGTTA+AGG | + | chr3.3:72956177-72956196 | None:intergenic | 15.0% |
| ! | AATAAACACAACCTTTGATA+TGG | + | chr3.3:72956092-72956111 | None:intergenic | 25.0% |
| ! | AATCAGACAAGTAATATTTG+AGG | + | chr3.3:72956695-72956714 | None:intergenic | 25.0% |
| AATATCATCGTGTAATACAC+CGG | - | chr3.3:72956542-72956561 | MS.gene75460:intron | 30.0% | |
| ACAAGTAATATTTGAGGAGT+TGG | + | chr3.3:72956689-72956708 | None:intergenic | 30.0% | |
| GTGTATTACACGATGATATT+GGG | + | chr3.3:72956542-72956561 | None:intergenic | 30.0% | |
| TATTGTGACGATTCTTGATT+AGG | - | chr3.3:72956369-72956388 | MS.gene75460:intron | 30.0% | |
| TGTAAAATGTACACATGATC+AGG | + | chr3.3:72956248-72956267 | None:intergenic | 30.0% | |
| TTTACCTTGGATGTAAAATG+TGG | + | chr3.3:72956148-72956167 | None:intergenic | 30.0% | |
| ! | CAGTTTTGCAAATATTGTCA+TGG | + | chr3.3:72956506-72956525 | None:intergenic | 30.0% |
| ACAGCTATGTTCCATATCAA+AGG | - | chr3.3:72956078-72956097 | MS.gene75460:CDS | 35.0% | |
| AGTAATATTTGAGGAGTTGG+TGG | + | chr3.3:72956686-72956705 | None:intergenic | 35.0% | |
| CACTGGAACATACAACATAA+TGG | - | chr3.3:72956467-72956486 | MS.gene75460:intron | 35.0% | |
| CATTATGTTGTATGTTCCAG+TGG | + | chr3.3:72956469-72956488 | None:intergenic | 35.0% | |
| CTTCACTATGCAAATGCTAT+GGG | - | chr3.3:72956627-72956646 | MS.gene75460:CDS | 35.0% | |
| GGTGTATTACACGATGATAT+TGG | + | chr3.3:72956543-72956562 | None:intergenic | 35.0% | |
| GTTAAGGTGTGTATTTACCT+TGG | + | chr3.3:72956161-72956180 | None:intergenic | 35.0% | |
| TAGCATTTGCATAGTGAAGA+TGG | + | chr3.3:72956626-72956645 | None:intergenic | 35.0% | |
| TCTTCACTATGCAAATGCTA+TGG | - | chr3.3:72956626-72956645 | MS.gene75460:CDS | 35.0% | |
| TTTGATATGGAACATAGCTG+TGG | + | chr3.3:72956079-72956098 | None:intergenic | 35.0% | |
| ! | AAATGCTATGGGTTACTTCA+AGG | - | chr3.3:72956638-72956657 | MS.gene75460:CDS | 35.0% |
| ! | AAGGATTGTGTTGCTGTTAA+TGG | - | chr3.3:72956020-72956039 | MS.gene75460:CDS | 35.0% |
| ! | TATGGACGAAGTTTTCTACA+CGG | - | chr3.3:72956428-72956447 | MS.gene75460:intron | 35.0% |
| AACATAGCTGTGGCAAGTAA+AGG | + | chr3.3:72956069-72956088 | None:intergenic | 40.0% | |
| ACATAGCTGTGGCAAGTAAA+GGG | + | chr3.3:72956068-72956087 | None:intergenic | 40.0% | |
| CGATTCTTGATTAGGTGCAA+AGG | - | chr3.3:72956377-72956396 | MS.gene75460:intron | 40.0% | |
| GTGCTCACTCACTACAAAAA+TGG | - | chr3.3:72956591-72956610 | MS.gene75460:CDS | 40.0% | |
| TCTTGATTAGGTGCAAAGGT+GGG | - | chr3.3:72956381-72956400 | MS.gene75460:intron | 40.0% | |
| TTCTTGATTAGGTGCAAAGG+TGG | - | chr3.3:72956380-72956399 | MS.gene75460:intron | 40.0% | |
| TTGCATAGTGAAGATGGTTG+AGG | + | chr3.3:72956620-72956639 | None:intergenic | 40.0% | |
| TTGGATGTAAAATGTGGCGT+TGG | + | chr3.3:72956142-72956161 | None:intergenic | 40.0% | |
| ! | AACGCCACATTTTACATCCA+AGG | - | chr3.3:72956141-72956160 | MS.gene75460:CDS | 40.0% |
| TAAAGGGAGGATAGAGCTGA+GGG | + | chr3.3:72956052-72956071 | None:intergenic | 45.0% | |
| TAGGTGCAAAGGTGGGAATT+CGG | - | chr3.3:72956388-72956407 | MS.gene75460:intron | 45.0% | |
| TATTGTGATACTTGCCGTGC+TGG | - | chr3.3:72956108-72956127 | MS.gene75460:CDS | 45.0% | |
| TGGTGGCAATATTGAGGCAA+AGG | + | chr3.3:72956669-72956688 | None:intergenic | 45.0% | |
| ! | GACGAAGTTTTCTACACGGA+AGG | - | chr3.3:72956432-72956451 | MS.gene75460:intron | 45.0% |
| !! | AGGAGTTGGTGGCAATATTG+AGG | + | chr3.3:72956675-72956694 | None:intergenic | 45.0% |
| CGTCCATAGTCTTCCTGTCT+TGG | + | chr3.3:72956416-72956435 | None:intergenic | 50.0% | |
| GAAGGAGTGACAGATTCCAC+TGG | - | chr3.3:72956450-72956469 | MS.gene75460:intron | 50.0% | |
| GAGGATAGAGCTGAGGGAAA+GGG | + | chr3.3:72956046-72956065 | None:intergenic | 50.0% | |
| GTAAAGGGAGGATAGAGCTG+AGG | + | chr3.3:72956053-72956072 | None:intergenic | 50.0% | |
| GTAGTGAGTGAGCACAATGC+TGG | + | chr3.3:72956586-72956605 | None:intergenic | 50.0% | |
| GTGCCAAGACAGGAAGACTA+TGG | - | chr3.3:72956410-72956429 | MS.gene75460:intron | 50.0% | |
| TAGCTGTGGCAAGTAAAGGG+AGG | + | chr3.3:72956065-72956084 | None:intergenic | 50.0% | |
| !! | CGTTGGTTTCGAATCCAGCA+CGG | + | chr3.3:72956125-72956144 | None:intergenic | 50.0% |
| ATGCTGGATCTGTTACGCCC+AGG | + | chr3.3:72956570-72956589 | None:intergenic | 55.0% | |
| CGTGTAATACACCGGACCCT+GGG | - | chr3.3:72956550-72956569 | MS.gene75460:intron | 55.0% | |
| GGAGGATAGAGCTGAGGGAA+AGG | + | chr3.3:72956047-72956066 | None:intergenic | 55.0% | |
| TCGTGTAATACACCGGACCC+TGG | - | chr3.3:72956549-72956568 | MS.gene75460:intron | 55.0% | |
| TGCTGGATCTGTTACGCCCA+GGG | + | chr3.3:72956569-72956588 | None:intergenic | 55.0% | |
| !! | TGGGAATTCGGTGCCAAGAC+AGG | - | chr3.3:72956400-72956419 | MS.gene75460:intron | 55.0% |
| GATCTGTTACGCCCAGGGTC+CGG | + | chr3.3:72956564-72956583 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 72956015 | 72956725 | 72956015 | ID=MS.gene75460 |
| chr3.3 | mRNA | 72956015 | 72956725 | 72956015 | ID=MS.gene75460.t1;Parent=MS.gene75460 |
| chr3.3 | exon | 72956578 | 72956725 | 72956578 | ID=MS.gene75460.t1.exon1;Parent=MS.gene75460.t1 |
| chr3.3 | CDS | 72956578 | 72956725 | 72956578 | ID=cds.MS.gene75460.t1;Parent=MS.gene75460.t1 |
| chr3.3 | exon | 72956015 | 72956349 | 72956015 | ID=MS.gene75460.t1.exon2;Parent=MS.gene75460.t1 |
| chr3.3 | CDS | 72956015 | 72956349 | 72956015 | ID=cds.MS.gene75460.t1;Parent=MS.gene75460.t1 |
| Gene Sequence |
| Protein sequence |
