AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76774


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76774.t1 MTR_0097s0070 96.020 201 8 0 1 201 1 201 1.85e-142 395
MS.gene76774.t1 MTR_8g089300 73.196 194 51 1 1 194 1 193 2.77e-94 273
MS.gene76774.t1 MTR_4g118810 36.986 146 86 3 29 171 35 177 4.65e-22 89.4
MS.gene76774.t1 MTR_7g108130 31.609 174 112 3 17 189 18 185 7.52e-21 85.9
MS.gene76774.t1 MTR_0004s0210 32.927 164 94 2 29 192 10 157 1.03e-20 84.7
MS.gene76774.t1 MTR_2g020060 28.659 164 106 3 31 189 27 184 5.62e-15 70.5
MS.gene76774.t1 MTR_7g011090 27.957 186 121 3 6 188 10 185 1.07e-13 67.0
MS.gene76774.t1 MTR_4g081880 30.189 159 96 4 31 185 33 180 4.97e-12 62.4
MS.gene76774.t1 MTR_5g041900 30.323 155 101 1 31 185 32 179 1.73e-11 60.8
MS.gene76774.t1 MTR_1g058540 33.562 146 84 4 31 173 42 177 7.86e-11 59.3
MS.gene76774.t1 MTR_2g086740 29.268 123 71 2 71 185 99 213 8.21e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76774.t1 AT5G44550 58.721 172 71 0 23 194 14 185 1.85e-66 202
MS.gene76774.t1 AT4G20390 55.882 170 74 1 22 190 12 181 5.07e-54 171
MS.gene76774.t1 AT4G15620 29.570 186 124 4 5 189 10 189 2.22e-18 79.3
MS.gene76774.t1 AT1G03700 28.659 164 101 3 29 192 11 158 8.07e-18 77.4
MS.gene76774.t1 AT4G15630 28.022 182 125 3 9 189 13 189 1.22e-17 77.4
MS.gene76774.t1 AT4G15610 30.556 144 93 3 31 172 33 171 5.54e-16 73.2
MS.gene76774.t1 AT1G14160 29.032 155 103 1 31 185 55 202 6.80e-16 73.2
MS.gene76774.t1 AT5G15290 30.769 143 90 3 31 172 32 166 3.75e-15 70.9
MS.gene76774.t1 AT3G06390 30.827 133 84 3 32 161 42 169 8.76e-14 67.4
MS.gene76774.t1 AT4G03540 29.008 131 77 3 44 174 26 140 4.39e-13 64.7

Find 42 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGGAGTTTGATTAAATT+TGG 0.215698 2.4:-43415739 None:intergenic
GCAGAAAGAGTAGCAGTTAT+TGG 0.256041 2.4:-43415717 None:intergenic
GCCCTTATTGCTTCTTTCAT+TGG 0.266507 2.4:+43416597 MS.gene76774:CDS
AAGTCCTTTGTAATCATATT+GGG 0.274380 2.4:-43416299 None:intergenic
ATGATTGCATTGGATATATT+AGG 0.293339 2.4:+43416274 MS.gene76774:CDS
CAGAAAGAGTAGCAGTTATT+GGG 0.306576 2.4:-43415716 None:intergenic
TACAAAATGGCTTCAAGAAA+TGG 0.318606 2.4:+43415522 None:intergenic
GAAGTCCTTTGTAATCATAT+TGG 0.328703 2.4:-43416300 None:intergenic
AGCCAATGAAAGAAGCAATA+AGG 0.340360 2.4:-43416599 None:intergenic
GGTGCCAATAACAAAACTTT+TGG 0.346894 2.4:-43415682 None:intergenic
TTTGAAAGCTATTGCAATAG+AGG 0.418883 2.4:+43416567 MS.gene76774:CDS
ATGTCAGCATTGGGAAGAAA+TGG 0.423404 2.4:+43416507 MS.gene76774:CDS
TTGAAAACTTACACAAAAGC+TGG 0.437745 2.4:-43415756 None:intergenic
GCCAATGAAAGAAGCAATAA+GGG 0.438011 2.4:-43416598 None:intergenic
ATGCAATCATCACCAAGTTA+TGG 0.467360 2.4:-43416261 None:intergenic
GGAGCATGGTTAATGCGATT+AGG 0.484616 2.4:-43416672 None:intergenic
AGCAACATTCATGTCAGCAT+TGG 0.488005 2.4:+43416497 MS.gene76774:CDS
ACTATGGCTCTGGCATCAGC+TGG 0.494373 2.4:+43416465 MS.gene76774:CDS
GCGATTAGGCTTGAGAAGCT+TGG 0.494528 2.4:-43416658 None:intergenic
GCAACATTCATGTCAGCATT+GGG 0.495269 2.4:+43416498 MS.gene76774:CDS
AAATGCTAGTCTCCATAACT+TGG 0.496794 2.4:+43416249 MS.gene76774:CDS
GGTGAAGTTAACTATGGCTC+TGG 0.500555 2.4:+43416455 MS.gene76774:intron
TGCAGCTGCAACACTTGTAA+TGG 0.510923 2.4:+43415644 MS.gene76774:CDS
ATGTTAATCATATCAAGGAC+TGG 0.510976 2.4:-43416694 None:intergenic
TGGTAATTCACATGCAAGAT+GGG 0.512674 2.4:+43416527 MS.gene76774:CDS
ATGGTAATTCACATGCAAGA+TGG 0.522902 2.4:+43416526 MS.gene76774:CDS
ATGGAGACTAGCATTTCCAT+TGG 0.523367 2.4:-43416242 None:intergenic
AGGTTCTTTGTGATAGCCAA+TGG 0.526185 2.4:+43416226 MS.gene76774:intron
AGCTATTGCAATAGAGGTGG+TGG 0.537163 2.4:+43416573 MS.gene76774:CDS
GCTCTGGCATCAGCTGGAGA+TGG 0.540036 2.4:+43416471 MS.gene76774:CDS
TAACTTGGTGATGATTGCAT+TGG 0.550469 2.4:+43416264 MS.gene76774:CDS
CATATCAAGGACTGGGAGCA+TGG 0.559515 2.4:-43416686 None:intergenic
TGTTAATCATATCAAGGACT+GGG 0.560345 2.4:-43416693 None:intergenic
AGGCTTGAGAAGCTTGGCAA+TGG 0.561801 2.4:-43416652 None:intergenic
TACAAGTGTTGCAGCTGCAG+TGG 0.571483 2.4:-43415640 None:intergenic
CTAAAATTGCAATAAATGCA+AGG 0.578209 2.4:-43416324 None:intergenic
GAAAGCTATTGCAATAGAGG+TGG 0.619990 2.4:+43416570 MS.gene76774:CDS
AGTTATTGGGGTGTTACCAA+CGG 0.622547 2.4:-43415703 None:intergenic
AGTCCTTTGTAATCATATTG+GGG 0.628572 2.4:-43416298 None:intergenic
AGAAAGAGTAGCAGTTATTG+GGG 0.633112 2.4:-43415715 None:intergenic
GGACCCCAATATGATTACAA+AGG 0.645480 2.4:+43416295 MS.gene76774:CDS
GGTGAAGAGAAAGTTGAACG+TGG 0.756724 2.4:+43415543 MS.gene76774:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATACATTTATATTAACAT+GGG + chr2.4:43416196-43416215 MS.gene76774:intron 10.0%
!! TTTATACATTTATATTAACA+TGG + chr2.4:43416195-43416214 MS.gene76774:intron 10.0%
!!! AAGTTTTTTAATTTTTTCTT+AGG + chr2.4:43416386-43416405 MS.gene76774:intron 10.0%
!! TATACATTTATATTAACATG+GGG + chr2.4:43416197-43416216 MS.gene76774:intron 15.0%
!! AAAGAAAAACAAAGAATGAT+TGG + chr2.4:43415583-43415602 MS.gene76774:CDS 20.0%
!!! AAACCAAAAAAGTTTTATGT+AGG + chr2.4:43415965-43415984 MS.gene76774:intron 20.0%
!!! ATTTATTGCAATTTTAGACA+TGG + chr2.4:43416330-43416349 MS.gene76774:CDS 20.0%
! AAGTCCTTTGTAATCATATT+GGG - chr2.4:43416302-43416321 None:intergenic 25.0%
! AATTTCATCGCTTAATTCAA+AGG + chr2.4:43416092-43416111 MS.gene76774:intron 25.0%
! ATGATTGCATTGGATATATT+AGG + chr2.4:43416274-43416293 MS.gene76774:CDS 25.0%
! CTAAAATTGCAATAAATGCA+AGG - chr2.4:43416327-43416346 None:intergenic 25.0%
!! ATTGGATTCTTTTATCTCTT+CGG + chr2.4:43415601-43415620 MS.gene76774:CDS 25.0%
ACGATTGAGAAAATAGAGAA+AGG + chr2.4:43416048-43416067 MS.gene76774:intron 30.0%
AGCTGGAGTTTGATTAAATT+TGG - chr2.4:43415742-43415761 None:intergenic 30.0%
AGTCCTTTGTAATCATATTG+GGG - chr2.4:43416301-43416320 None:intergenic 30.0%
GAAGTCCTTTGTAATCATAT+TGG - chr2.4:43416303-43416322 None:intergenic 30.0%
TTGAAAACTTACACAAAAGC+TGG - chr2.4:43415759-43415778 None:intergenic 30.0%
TTTGAAAGCTATTGCAATAG+AGG + chr2.4:43416567-43416586 MS.gene76774:CDS 30.0%
! TTGGATTCTTTTATCTCTTC+GGG + chr2.4:43415602-43415621 MS.gene76774:CDS 30.0%
!! CCAAAAAAGTTTTATGTAGG+AGG + chr2.4:43415968-43415987 MS.gene76774:intron 30.0%
!!! CAGACCAAAAGTTTTGTTAT+TGG + chr2.4:43415678-43415697 MS.gene76774:CDS 30.0%
!!! CCTCCTACATAAAACTTTTT+TGG - chr2.4:43415971-43415990 None:intergenic 30.0%
!!! TTTTTGGGTGAAGTTAACTA+TGG + chr2.4:43416449-43416468 MS.gene76774:intron 30.0%
AGAAAGAGTAGCAGTTATTG+GGG - chr2.4:43415718-43415737 None:intergenic 35.0%
AGCCAATGAAAGAAGCAATA+AGG - chr2.4:43416602-43416621 None:intergenic 35.0%
ATACCATTGTACTACTCGTT+CGG - chr2.4:43415937-43415956 None:intergenic 35.0%
ATATTAACATGGGGTGATAC+AGG + chr2.4:43416206-43416225 MS.gene76774:intron 35.0%
ATGCAATCATCACCAAGTTA+TGG - chr2.4:43416264-43416283 None:intergenic 35.0%
ATGGTAATTCACATGCAAGA+TGG + chr2.4:43416526-43416545 MS.gene76774:CDS 35.0%
CAGAAAGAGTAGCAGTTATT+GGG - chr2.4:43415719-43415738 None:intergenic 35.0%
GCCAATGAAAGAAGCAATAA+GGG - chr2.4:43416601-43416620 None:intergenic 35.0%
TAGTAGAAGAGGTCGAATAT+CGG - chr2.4:43415995-43416014 None:intergenic 35.0%
TGGTAATTCACATGCAAGAT+GGG + chr2.4:43416527-43416546 MS.gene76774:CDS 35.0%
! AAATGCTAGTCTCCATAACT+TGG + chr2.4:43416249-43416268 MS.gene76774:CDS 35.0%
! CTCTATTTTCTCAATCGTAG+TGG - chr2.4:43416046-43416065 None:intergenic 35.0%
! GGTGCCAATAACAAAACTTT+TGG - chr2.4:43415685-43415704 None:intergenic 35.0%
!! ATCGTAGTGGTTAATTTGTC+AGG - chr2.4:43416033-43416052 None:intergenic 35.0%
!! TAACTTGGTGATGATTGCAT+TGG + chr2.4:43416264-43416283 MS.gene76774:CDS 35.0%
!! TCGTAGTGGTTAATTTGTCA+GGG - chr2.4:43416032-43416051 None:intergenic 35.0%
AGCAACATTCATGTCAGCAT+TGG + chr2.4:43416497-43416516 MS.gene76774:CDS 40.0%
AGGTTCTTTGTGATAGCCAA+TGG + chr2.4:43416226-43416245 MS.gene76774:intron 40.0%
ATGGAGACTAGCATTTCCAT+TGG - chr2.4:43416245-43416264 None:intergenic 40.0%
GAAAGCTATTGCAATAGAGG+TGG + chr2.4:43416570-43416589 MS.gene76774:CDS 40.0%
GAGGGAAATGTTAGTAGAAG+AGG - chr2.4:43416006-43416025 None:intergenic 40.0%
GCAACATTCATGTCAGCATT+GGG + chr2.4:43416498-43416517 MS.gene76774:CDS 40.0%
GCAGAAAGAGTAGCAGTTAT+TGG - chr2.4:43415720-43415739 None:intergenic 40.0%
GCCCTTATTGCTTCTTTCAT+TGG + chr2.4:43416597-43416616 MS.gene76774:CDS 40.0%
GGACCCCAATATGATTACAA+AGG + chr2.4:43416295-43416314 MS.gene76774:CDS 40.0%
TACCATTGTACTACTCGTTC+GGG - chr2.4:43415936-43415955 None:intergenic 40.0%
! AGTTATTGGGGTGTTACCAA+CGG - chr2.4:43415706-43415725 None:intergenic 40.0%
! ATGTCAGCATTGGGAAGAAA+TGG + chr2.4:43416507-43416526 MS.gene76774:CDS 40.0%
! GATTCTTTTATCTCTTCGGG+TGG + chr2.4:43415605-43415624 MS.gene76774:CDS 40.0%
!! GTTAATTTGTCAGGGAGAGA+GGG - chr2.4:43416024-43416043 None:intergenic 40.0%
!!! AGTTTTGTTATTGGCACCGT+TGG + chr2.4:43415687-43415706 MS.gene76774:CDS 40.0%
ACCATTGTACTACTCGTTCG+GGG - chr2.4:43415935-43415954 None:intergenic 45.0%
AGCTATTGCAATAGAGGTGG+TGG + chr2.4:43416573-43416592 MS.gene76774:CDS 45.0%
GGAGCATGGTTAATGCGATT+AGG - chr2.4:43416675-43416694 None:intergenic 45.0%
GGTGAAGAGAAAGTTGAACG+TGG + chr2.4:43415543-43415562 MS.gene76774:CDS 45.0%
GGTGAAGTTAACTATGGCTC+TGG + chr2.4:43416455-43416474 MS.gene76774:intron 45.0%
TGCAGCTGCAACACTTGTAA+TGG + chr2.4:43415644-43415663 MS.gene76774:CDS 45.0%
!! GGTTAATTTGTCAGGGAGAG+AGG - chr2.4:43416025-43416044 None:intergenic 45.0%
!! ATCTATAATAAAATTAAAAT+AGG - chr2.4:43416174-43416193 None:intergenic 5.0%
!!! ATTGATAAATAATATTTTTT+TGG + chr2.4:43416433-43416452 MS.gene76774:intron 5.0%
!!! TTGATAAATAATATTTTTTT+GGG + chr2.4:43416434-43416453 MS.gene76774:intron 5.0%
AGGCTTGAGAAGCTTGGCAA+TGG - chr2.4:43416655-43416674 None:intergenic 50.0%
GCGATTAGGCTTGAGAAGCT+TGG - chr2.4:43416661-43416680 None:intergenic 50.0%
TCCCCGAACGAGTAGTACAA+TGG + chr2.4:43415931-43415950 MS.gene76774:intron 50.0%
! TACAAGTGTTGCAGCTGCAG+TGG - chr2.4:43415643-43415662 None:intergenic 50.0%
!! ACTATGGCTCTGGCATCAGC+TGG + chr2.4:43416465-43416484 MS.gene76774:CDS 55.0%
GCTCTGGCATCAGCTGGAGA+TGG + chr2.4:43416471-43416490 MS.gene76774:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 43415528 43416704 43415528 ID=MS.gene76774
chr2.4 mRNA 43415528 43416704 43415528 ID=MS.gene76774.t1;Parent=MS.gene76774
chr2.4 exon 43415528 43415766 43415528 ID=MS.gene76774.t1.exon1;Parent=MS.gene76774.t1
chr2.4 CDS 43415528 43415766 43415528 ID=cds.MS.gene76774.t1;Parent=MS.gene76774.t1
chr2.4 exon 43416228 43416351 43416228 ID=MS.gene76774.t1.exon2;Parent=MS.gene76774.t1
chr2.4 CDS 43416228 43416351 43416228 ID=cds.MS.gene76774.t1;Parent=MS.gene76774.t1
chr2.4 exon 43416462 43416704 43416462 ID=MS.gene76774.t1.exon3;Parent=MS.gene76774.t1
chr2.4 CDS 43416462 43416704 43416462 ID=cds.MS.gene76774.t1;Parent=MS.gene76774.t1
Gene Sequence

>MS.gene76774

ATGGCTTCAAGAAATGGTGAAGAGAAAGTTGAACGTGGCTTCAATGATGTTCATGAAAGAAAAACAAAGAATGATTGGATTCTTTTATCTCTTCGGGTGGCTGCATTTTTTGCCACTGCAGCTGCAACACTTGTAATGGCACTCAACAAACAGACCAAAAGTTTTGTTATTGGCACCGTTGGTAACACCCCAATAACTGCTACTCTTTCTGCCAAATTTAATCAAACTCCAGCTTTTGTGTAAGTTTTCAATAATTATATATATATATATATATATATATATATATATATATATATATATATATATATATACACACAAATATCTATGATTCTACTTTTCTCTTTTTTATTTATGTTCGTTTGCAATGACTACTAGAAAAAAAATATAAGTAGTAACAATAGAGTCCCCGAACGAGTAGTACAATGGTATCGATTCATAAACCAAAAAAGTTTTATGTAGGAGGTCCGATATTCGACCTCTTCTACTAACATTTCCCTCTCTCCCTGACAAATTAACCACTACGATTGAGAAAATAGAGAAAGGAAATTGTGATGATCTATCTCTAATTTCATCGCTTAATTCAAAGGCGATGAATTTAAAGATATGTTTATTATTTTTTTATTTTTATTTTTGTGATGATATTCCTATTTTAATTTTATTATAGATTTTTATACATTTATATTAACATGGGGTGATACAGGTTCTTTGTGATAGCCAATGGAAATGCTAGTCTCCATAACTTGGTGATGATTGCATTGGATATATTAGGACCCCAATATGATTACAAAGGACTTCGCCTTGCATTTATTGCAATTTTAGACATGGTAATTTTTTTAATTAATTTTACATCAATTCTACAAGTTTTTTAATTTTTTCTTAGGATATCATTTATGTAAGAAAAAATGATTGATAAATAATATTTTTTTGGGTGAAGTTAACTATGGCTCTGGCATCAGCTGGAGATGGTGCAGCAACATTCATGTCAGCATTGGGAAGAAATGGTAATTCACATGCAAGATGGGATAAGATTTGTGACAAATTTGAAAGCTATTGCAATAGAGGTGGTGGCGCCCTTATTGCTTCTTTCATTGGCTTCATTCTTCTCTTGATTATCACTGTGATGTCCATTGCCAAGCTTCTCAAGCCTAATCGCATTAACCATGCTCCCAGTCCTTGA

Protein sequence

>MS.gene76774.t1

MASRNGEEKVERGFNDVHERKTKNDWILLSLRVAAFFATAAATLVMALNKQTKSFVIGTVGNTPITATLSAKFNQTPAFVFFVIANGNASLHNLVMIALDILGPQYDYKGLRLAFIAILDMLTMALASAGDGAATFMSALGRNGNSHARWDKICDKFESYCNRGGGALIASFIGFILLLIITVMSIAKLLKPNRINHAPSP