Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059070.t1 | XP_013443594.1 | 99.1 | 111 | 1 | 0 | 1 | 111 | 53 | 163 | 1.20E-48 | 202.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059070.t1 | B9RW00 | 71.2 | 111 | 32 | 0 | 1 | 111 | 53 | 163 | 7.9e-36 | 151.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059070.t1 | A0A072TJ77 | 99.1 | 111 | 1 | 0 | 1 | 111 | 53 | 163 | 9.0e-49 | 202.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051138 | MS.gene059070 | 0.805674 | 1.19E-49 | -1.69E-46 |
| MS.gene051422 | MS.gene059070 | 0.822577 | 2.20E-53 | -1.69E-46 |
| MS.gene051651 | MS.gene059070 | 0.827907 | 1.21E-54 | -1.69E-46 |
| MS.gene056856 | MS.gene059070 | 0.800558 | 1.36E-48 | -1.69E-46 |
| MS.gene057717 | MS.gene059070 | 0.809477 | 1.86E-50 | -1.69E-46 |
| MS.gene058067 | MS.gene059070 | 0.813547 | 2.42E-51 | -1.69E-46 |
| MS.gene058068 | MS.gene059070 | 0.813703 | 2.24E-51 | -1.69E-46 |
| MS.gene059069 | MS.gene059070 | 0.988023 | 2.03E-172 | -1.69E-46 |
| MS.gene059070 | MS.gene061777 | 0.806767 | 7.01E-50 | -1.69E-46 |
| MS.gene059070 | MS.gene062572 | 0.807157 | 5.80E-50 | -1.69E-46 |
| MS.gene059070 | MS.gene065424 | 0.818279 | 2.13E-52 | -1.69E-46 |
| MS.gene059070 | MS.gene068037 | 0.822806 | 1.94E-53 | -1.69E-46 |
| MS.gene059070 | MS.gene072979 | 0.816824 | 4.53E-52 | -1.69E-46 |
| MS.gene059070 | MS.gene09819 | 0.803336 | 3.66E-49 | -1.69E-46 |
| MS.gene059070 | MS.gene21794 | 0.805983 | 1.03E-49 | -1.69E-46 |
| MS.gene059070 | MS.gene24540 | 0.802413 | 5.68E-49 | -1.69E-46 |
| MS.gene059070 | MS.gene24882 | 0.816531 | 5.27E-52 | -1.69E-46 |
| MS.gene059070 | MS.gene28077 | 0.801953 | 7.06E-49 | -1.69E-46 |
| MS.gene059070 | MS.gene32446 | 0.803347 | 3.64E-49 | -1.69E-46 |
| MS.gene059070 | MS.gene33505 | 0.800003 | 1.77E-48 | -1.69E-46 |
| MS.gene059070 | MS.gene39959 | 0.833357 | 5.59E-56 | -1.69E-46 |
| MS.gene059070 | MS.gene40492 | 0.800237 | 1.58E-48 | -1.69E-46 |
| MS.gene059070 | MS.gene44877 | 0.812032 | 5.20E-51 | -1.69E-46 |
| MS.gene059070 | MS.gene45776 | 0.800722 | 1.26E-48 | -1.69E-46 |
| MS.gene059070 | MS.gene47576 | 0.800506 | 1.40E-48 | -1.69E-46 |
| MS.gene059070 | MS.gene48360 | 0.811305 | 7.48E-51 | -1.69E-46 |
| MS.gene059070 | MS.gene50534 | 0.829101 | 6.22E-55 | -1.69E-46 |
| MS.gene059070 | MS.gene53903 | 0.983605 | 3.34E-158 | -1.69E-46 |
| MS.gene059070 | MS.gene57291 | 0.806427 | 8.27E-50 | -1.69E-46 |
| MS.gene059070 | MS.gene58425 | 0.82381 | 1.13E-53 | -1.69E-46 |
| MS.gene059070 | MS.gene63947 | 0.886419 | 3.48E-72 | -1.69E-46 |
| MS.gene059070 | MS.gene64069 | 0.806881 | 6.63E-50 | -1.69E-46 |
| MS.gene059070 | MS.gene71606 | 0.818316 | 2.09E-52 | -1.69E-46 |
| MS.gene059070 | MS.gene75261 | 0.81782 | 2.70E-52 | -1.69E-46 |
| MS.gene059070 | MS.gene76774 | 0.812414 | 4.29E-51 | -1.69E-46 |
| MS.gene059070 | MS.gene78768 | 0.828226 | 1.01E-54 | -1.69E-46 |
| MS.gene059070 | MS.gene81725 | 0.814761 | 1.31E-51 | -1.69E-46 |
| MS.gene059070 | MS.gene84585 | 0.808137 | 3.59E-50 | -1.69E-46 |
| MS.gene059070 | MS.gene84947 | 0.803945 | 2.74E-49 | -1.69E-46 |
| MS.gene059070 | MS.gene85638 | 0.819502 | 1.12E-52 | -1.69E-46 |
| MS.gene059070 | MS.gene91317 | 0.859556 | 3.81E-63 | -1.69E-46 |
| MS.gene059070 | MS.gene95132 | 0.814036 | 1.89E-51 | -1.69E-46 |
| MS.gene059070 | MS.gene96299 | 0.805903 | 1.07E-49 | -1.69E-46 |
| MS.gene059070 | MS.gene96701 | 0.850589 | 1.54E-60 | -1.69E-46 |
| MS.gene059070 | MS.gene97682 | 0.810111 | 1.36E-50 | -1.69E-46 |
| MS.gene059070 | MS.gene98021 | 0.817097 | 3.94E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTCATGTGATTGCAAAATT+TGG | 0.163683 | 4.2:-60968485 | None:intergenic |
| AACTCTATTGTAACTGTCTA+TGG | 0.255815 | 4.2:+60967507 | MS.gene059070:CDS |
| ACTTGTGGTCGCCATGGATA+TGG | 0.315968 | 4.2:+60967572 | MS.gene059070:CDS |
| GCTTTAGTCGCTAGTTTCAT+TGG | 0.393377 | 4.2:+60968511 | MS.gene059070:CDS |
| CTCAAGCATCTCTGCAGCTT+TGG | 0.400682 | 4.2:+60968396 | MS.gene059070:CDS |
| GTCATGTGATTGCAAAATTT+GGG | 0.430792 | 4.2:-60968484 | None:intergenic |
| CGCCAGAGCAAGCTTTCTGC+AGG | 0.458432 | 4.2:-60967549 | None:intergenic |
| GCTGCTTGGCTACCGATTTG+TGG | 0.463642 | 4.2:+60968454 | MS.gene059070:CDS |
| TTCCTGCAGAAAGCTTGCTC+TGG | 0.469885 | 4.2:+60967547 | MS.gene059070:CDS |
| ATGGAAACAATTATGCTGCT+TGG | 0.504739 | 4.2:+60968440 | MS.gene059070:CDS |
| ATAGCTCAAGTGGCAAAGAA+TGG | 0.508607 | 4.2:+60968421 | MS.gene059070:CDS |
| TTGGGAACTGAACCACAAAT+CGG | 0.511633 | 4.2:-60968466 | None:intergenic |
| AGGGTCAAGGACAGTATGAA+TGG | 0.516263 | 4.2:-60968566 | None:intergenic |
| AAGCTTGCTCTGGCGACTTG+TGG | 0.523109 | 4.2:+60967557 | MS.gene059070:CDS |
| TTCTCAAAAGGAGAGGGTCA+AGG | 0.544463 | 4.2:-60968579 | None:intergenic |
| CTGGCGACTTGTGGTCGCCA+TGG | 0.548545 | 4.2:+60967566 | MS.gene059070:CDS |
| AGCTTTGGCTATAGCTCAAG+TGG | 0.581117 | 4.2:+60968411 | MS.gene059070:CDS |
| TTAGGACATTACCATATCCA+TGG | 0.585943 | 4.2:-60967583 | None:intergenic |
| AGCTTTCTGCAGGAAGAAAG+AGG | 0.586262 | 4.2:-60967539 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAAATGCTAAATTATTA+TGG | + | chr4.2:60968304-60968323 | MS.gene059070:intron | 15.0% |
| !!! | ATTTTATATGATCAAACTTA+GGG | - | chr4.2:60968095-60968114 | None:intergenic | 15.0% |
| !!! | GTTTTATTTTTATGGATTTA+AGG | - | chr4.2:60968025-60968044 | None:intergenic | 15.0% |
| !!! | TATTTTATATGATCAAACTT+AGG | - | chr4.2:60968096-60968115 | None:intergenic | 15.0% |
| !! | AAGAACAAATGTTTCAAATT+TGG | - | chr4.2:60967697-60967716 | None:intergenic | 20.0% |
| !! | ATGCTAAATTATTATGGTTA+TGG | + | chr4.2:60968310-60968329 | MS.gene059070:intron | 20.0% |
| !! | CAAACATTATATGACTTAAA+TGG | - | chr4.2:60967827-60967846 | None:intergenic | 20.0% |
| !!! | ATGAGAAGGTTTTATTTTTA+TGG | - | chr4.2:60968033-60968052 | None:intergenic | 20.0% |
| !!! | TATTTTTATGGATTTAAGGT+TGG | - | chr4.2:60968021-60968040 | None:intergenic | 20.0% |
| !!! | TGTTCTTCATGTTTTTAAAA+TGG | + | chr4.2:60967710-60967729 | MS.gene059070:intron | 20.0% |
| !!! | TTTAATTGATTTTTATGAGC+AGG | + | chr4.2:60968354-60968373 | MS.gene059070:intron | 20.0% |
| !!! | TTTTCGTTTTTTCATTTCAT+TGG | - | chr4.2:60968286-60968305 | None:intergenic | 20.0% |
| ! | ATGATAAAGAGGTTAACTTT+AGG | - | chr4.2:60967604-60967623 | None:intergenic | 25.0% |
| ! | ATTAGAATACTAGTTATGTC+TGG | + | chr4.2:60968140-60968159 | MS.gene059070:intron | 25.0% |
| ! | GACATAACTAGTATTCTAAT+AGG | - | chr4.2:60968140-60968159 | None:intergenic | 25.0% |
| ! | TAAGTCATATAATGTTTGCA+TGG | + | chr4.2:60967829-60967848 | MS.gene059070:intron | 25.0% |
| ! | TATGAACTCTTTAACATAGT+TGG | - | chr4.2:60967869-60967888 | None:intergenic | 25.0% |
| ! | TATGTTAAAGAGTTCATACA+TGG | + | chr4.2:60967871-60967890 | MS.gene059070:intron | 25.0% |
| !! | CTAACCAATGTATGATTTTT+CGG | + | chr4.2:60968208-60968227 | MS.gene059070:intron | 25.0% |
| !!! | TCTTCATGTTTTTAAAATGG+AGG | + | chr4.2:60967713-60967732 | MS.gene059070:intron | 25.0% |
| AAAGTTCTTGCATGATAAAG+AGG | - | chr4.2:60967615-60967634 | None:intergenic | 30.0% | |
| AACACGTCAAACAATAAACT+TGG | - | chr4.2:60968247-60968266 | None:intergenic | 30.0% | |
| AACTCTATTGTAACTGTCTA+TGG | + | chr4.2:60967507-60967526 | MS.gene059070:CDS | 30.0% | |
| AACTCTTTAACATAGTTGGA+TGG | - | chr4.2:60967865-60967884 | None:intergenic | 30.0% | |
| GTCATGTGATTGCAAAATTT+GGG | - | chr4.2:60968487-60968506 | None:intergenic | 30.0% | |
| TGTCATGTGATTGCAAAATT+TGG | - | chr4.2:60968488-60968507 | None:intergenic | 30.0% | |
| ! | AAATTTTGCAATCACATGAC+AGG | + | chr4.2:60968487-60968506 | MS.gene059070:CDS | 30.0% |
| ! | AAGTTTTTCTCAAAAGGAGA+GGG | - | chr4.2:60968588-60968607 | None:intergenic | 30.0% |
| ! | GCATTTTTGTTAGTTGAGTT+AGG | + | chr4.2:60967782-60967801 | MS.gene059070:intron | 30.0% |
| ! | TGTAACTGTCTATGGATTTT+TGG | + | chr4.2:60967515-60967534 | MS.gene059070:CDS | 30.0% |
| !!! | TGTTTTTAAAATGGAGGTGT+TGG | + | chr4.2:60967719-60967738 | MS.gene059070:intron | 30.0% |
| ATGGAAACAATTATGCTGCT+TGG | + | chr4.2:60968440-60968459 | MS.gene059070:CDS | 35.0% | |
| GAATACTAGTTATGTCTGGA+TGG | + | chr4.2:60968144-60968163 | MS.gene059070:intron | 35.0% | |
| GTAAAGTCAGACGTTATGAT+CGG | - | chr4.2:60967755-60967774 | None:intergenic | 35.0% | |
| TAAAGTCAGACGTTATGATC+GGG | - | chr4.2:60967754-60967773 | None:intergenic | 35.0% | |
| TCAGCCGAAAAATCATACAT+TGG | - | chr4.2:60968215-60968234 | None:intergenic | 35.0% | |
| TGGATGGAGATTAAGTCTAT+AGG | + | chr4.2:60968160-60968179 | MS.gene059070:intron | 35.0% | |
| TTAGGACATTACCATATCCA+TGG | - | chr4.2:60967586-60967605 | None:intergenic | 35.0% | |
| ! | ACATTGGTTAGTCACACATT+GGG | - | chr4.2:60968199-60968218 | None:intergenic | 35.0% |
| ! | CAAGTTTTTCTCAAAAGGAG+AGG | - | chr4.2:60968589-60968608 | None:intergenic | 35.0% |
| ! | TACATTGGTTAGTCACACAT+TGG | - | chr4.2:60968200-60968219 | None:intergenic | 35.0% |
| !! | TATGGATTTAAGGTTGGTGA+TGG | - | chr4.2:60968015-60968034 | None:intergenic | 35.0% |
| AAAGTCAGACGTTATGATCG+GGG | - | chr4.2:60967753-60967772 | None:intergenic | 40.0% | |
| ATAGCTCAAGTGGCAAAGAA+TGG | + | chr4.2:60968421-60968440 | MS.gene059070:CDS | 40.0% | |
| TTGGGAACTGAACCACAAAT+CGG | - | chr4.2:60968469-60968488 | None:intergenic | 40.0% | |
| ! | GCTTTAGTCGCTAGTTTCAT+TGG | + | chr4.2:60968511-60968530 | MS.gene059070:CDS | 40.0% |
| AGCGTGTTTGAGAGATGAGA+AGG | - | chr4.2:60968047-60968066 | None:intergenic | 45.0% | |
| AGCTTTCTGCAGGAAGAAAG+AGG | - | chr4.2:60967542-60967561 | None:intergenic | 45.0% | |
| AGGGTCAAGGACAGTATGAA+TGG | - | chr4.2:60968569-60968588 | None:intergenic | 45.0% | |
| TTCTCAAAAGGAGAGGGTCA+AGG | - | chr4.2:60968582-60968601 | None:intergenic | 45.0% | |
| ! | AGCTTTGGCTATAGCTCAAG+TGG | + | chr4.2:60968411-60968430 | MS.gene059070:CDS | 45.0% |
| ACTTGTGGTCGCCATGGATA+TGG | + | chr4.2:60967572-60967591 | MS.gene059070:CDS | 50.0% | |
| CTCAAGCATCTCTGCAGCTT+TGG | + | chr4.2:60968396-60968415 | MS.gene059070:CDS | 50.0% | |
| ! | TTCCTGCAGAAAGCTTGCTC+TGG | + | chr4.2:60967547-60967566 | MS.gene059070:CDS | 50.0% |
| ! | AAGCTTGCTCTGGCGACTTG+TGG | + | chr4.2:60967557-60967576 | MS.gene059070:CDS | 55.0% |
| !! | GCTGCTTGGCTACCGATTTG+TGG | + | chr4.2:60968454-60968473 | MS.gene059070:CDS | 55.0% |
| CGCCAGAGCAAGCTTTCTGC+AGG | - | chr4.2:60967552-60967571 | None:intergenic | 60.0% | |
| CTGGCGACTTGTGGTCGCCA+TGG | + | chr4.2:60967566-60967585 | MS.gene059070:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 60967491 | 60968609 | 60967491 | ID=MS.gene059070 |
| chr4.2 | mRNA | 60967491 | 60968609 | 60967491 | ID=MS.gene059070.t1;Parent=MS.gene059070 |
| chr4.2 | exon | 60967491 | 60967593 | 60967491 | ID=MS.gene059070.t1.exon1;Parent=MS.gene059070.t1 |
| chr4.2 | CDS | 60967491 | 60967593 | 60967491 | ID=cds.MS.gene059070.t1;Parent=MS.gene059070.t1 |
| chr4.2 | exon | 60968376 | 60968609 | 60968376 | ID=MS.gene059070.t1.exon2;Parent=MS.gene059070.t1 |
| chr4.2 | CDS | 60968376 | 60968609 | 60968376 | ID=cds.MS.gene059070.t1;Parent=MS.gene059070.t1 |
| Gene Sequence |
| Protein sequence |
