Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79220.t1 | XP_003601958.1 | 88.9 | 199 | 22 | 0 | 1 | 199 | 1 | 199 | 3.60E-91 | 344.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79220.t1 | G7J3S4 | 88.9 | 199 | 22 | 0 | 1 | 199 | 1 | 199 | 2.6e-91 | 344.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene79220 | 0.862968 | 3.48E-64 | -1.69E-46 |
| MS.gene052211 | MS.gene79220 | 0.890668 | 8.00E-74 | -1.69E-46 |
| MS.gene052212 | MS.gene79220 | 0.821132 | 4.75E-53 | -1.69E-46 |
| MS.gene05240 | MS.gene79220 | 0.802902 | 4.50E-49 | -1.69E-46 |
| MS.gene052606 | MS.gene79220 | 0.810427 | 1.16E-50 | -1.69E-46 |
| MS.gene053403 | MS.gene79220 | 0.827208 | 1.78E-54 | -1.69E-46 |
| MS.gene053406 | MS.gene79220 | 0.849031 | 4.20E-60 | -1.69E-46 |
| MS.gene053420 | MS.gene79220 | 0.800304 | 1.53E-48 | -1.69E-46 |
| MS.gene054146 | MS.gene79220 | 0.870866 | 1.06E-66 | -1.69E-46 |
| MS.gene054147 | MS.gene79220 | 0.803257 | 3.80E-49 | -1.69E-46 |
| MS.gene054421 | MS.gene79220 | 0.825503 | 4.53E-54 | -1.69E-46 |
| MS.gene054742 | MS.gene79220 | 0.829314 | 5.52E-55 | -1.69E-46 |
| MS.gene055124 | MS.gene79220 | 0.801774 | 7.68E-49 | -1.69E-46 |
| MS.gene055154 | MS.gene79220 | 0.821436 | 4.04E-53 | -1.69E-46 |
| MS.gene055240 | MS.gene79220 | 0.885577 | 7.21E-72 | -1.69E-46 |
| MS.gene056076 | MS.gene79220 | 0.800093 | 1.69E-48 | -1.69E-46 |
| MS.gene056218 | MS.gene79220 | 0.81716 | 3.81E-52 | -1.69E-46 |
| MS.gene056224 | MS.gene79220 | 0.804521 | 2.08E-49 | -1.69E-46 |
| MS.gene056590 | MS.gene79220 | 0.811775 | 5.91E-51 | -1.69E-46 |
| MS.gene056591 | MS.gene79220 | 0.811029 | 8.59E-51 | -1.69E-46 |
| MS.gene056748 | MS.gene79220 | 0.818904 | 1.54E-52 | -1.69E-46 |
| MS.gene056920 | MS.gene79220 | 0.815913 | 7.25E-52 | -1.69E-46 |
| MS.gene058177 | MS.gene79220 | 0.804016 | 2.64E-49 | -1.69E-46 |
| MS.gene058603 | MS.gene79220 | 0.82767 | 1.38E-54 | -1.69E-46 |
| MS.gene059953 | MS.gene79220 | 0.815197 | 1.05E-51 | -1.69E-46 |
| MS.gene059955 | MS.gene79220 | 0.833771 | 4.40E-56 | -1.69E-46 |
| MS.gene060087 | MS.gene79220 | 0.830139 | 3.47E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79220.t1 | MTR_3g087270 | 93.467 | 199 | 13 | 0 | 1 | 199 | 1 | 199 | 3.11e-140 | 389 |
| MS.gene79220.t1 | MTR_3g087278 | 88.945 | 199 | 22 | 0 | 1 | 199 | 1 | 199 | 3.55e-131 | 366 |
| MS.gene79220.t1 | MTR_3g087280 | 87.437 | 199 | 25 | 0 | 1 | 199 | 1 | 199 | 4.52e-131 | 366 |
| MS.gene79220.t1 | MTR_3g087330 | 84.422 | 199 | 31 | 0 | 1 | 199 | 1 | 199 | 4.11e-127 | 356 |
| MS.gene79220.t1 | MTR_3g087325 | 85.427 | 199 | 29 | 0 | 1 | 199 | 1 | 199 | 3.30e-125 | 351 |
| MS.gene79220.t1 | MTR_3g087275 | 85.427 | 199 | 28 | 1 | 1 | 199 | 1 | 198 | 7.50e-123 | 345 |
| MS.gene79220.t1 | MTR_3g087300 | 65.482 | 197 | 24 | 3 | 1 | 197 | 1 | 153 | 2.76e-85 | 251 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79220.t1 | AT5G65030 | 30.964 | 197 | 124 | 3 | 11 | 199 | 10 | 202 | 4.14e-26 | 99.8 |
| MS.gene79220.t1 | AT5G10210 | 31.633 | 196 | 112 | 7 | 15 | 199 | 13 | 197 | 4.97e-24 | 94.4 |
Find 34 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTACCAAATCAGTACCATTT+TGG | 0.165786 | 3.1:-70240562 | MS.gene79220:CDS |
| ATAAGAGTAGGTTGAATAAT+TGG | 0.258714 | 3.1:-70240247 | MS.gene79220:CDS |
| TTAATCTCCACTTCTACTTC+CGG | 0.353807 | 3.1:+70240308 | None:intergenic |
| TGCGCTTAGCGCAGCTTTGG+AGG | 0.362485 | 3.1:-70240156 | MS.gene79220:CDS |
| TTAAATGACTCGTCCCAAAA+TGG | 0.387620 | 3.1:+70240548 | None:intergenic |
| GCTCCAACACTAGGCTTTGT+TGG | 0.406615 | 3.1:+70240483 | None:intergenic |
| AGTGGCTACGGTTCATGAAA+TGG | 0.409468 | 3.1:-70240279 | MS.gene79220:CDS |
| GTCCCAAAATGGTACTGATT+TGG | 0.410434 | 3.1:+70240559 | None:intergenic |
| ATTTGCGCTTAGCGCAGCTT+TGG | 0.427353 | 3.1:-70240159 | MS.gene79220:CDS |
| GATTCTAGAATCACTTTCCA+TGG | 0.440184 | 3.1:+70240410 | None:intergenic |
| GCAAGGCTGTATCTTCCAAC+AGG | 0.447411 | 3.1:-70240623 | MS.gene79220:CDS |
| TGCACCAAGCGCGATGATCA+TGG | 0.461361 | 3.1:+70240201 | None:intergenic |
| TTGGAAAAGGTGAGATTCCA+TGG | 0.470388 | 3.1:-70240427 | MS.gene79220:CDS |
| AAAGCCTAGTGTTGGAGCTA+AGG | 0.499139 | 3.1:-70240478 | MS.gene79220:CDS |
| TTTGCCTTAGCTCCAACACT+AGG | 0.507447 | 3.1:+70240474 | None:intergenic |
| CAACTGAAGTTTGCATATGC+AGG | 0.509989 | 3.1:+70240707 | None:intergenic |
| GGGTCACAACTTATTGGAAA+AGG | 0.536727 | 3.1:-70240440 | MS.gene79220:CDS |
| AGTGGAGATTAAAATAAGAG+TGG | 0.548596 | 3.1:-70240297 | MS.gene79220:CDS |
| AATGAGTGTGGATGCAAGAA+TGG | 0.549203 | 3.1:-70240224 | MS.gene79220:CDS |
| ATGGCCATGATCATCGCGCT+TGG | 0.550412 | 3.1:-70240205 | MS.gene79220:CDS |
| GATTAAAATAAGAGTGGCTA+CGG | 0.551977 | 3.1:-70240291 | MS.gene79220:CDS |
| GCTAAGGCAAAGGAAGATGT+GGG | 0.557016 | 3.1:-70240462 | MS.gene79220:CDS |
| AGCTAAGGCAAAGGAAGATG+TGG | 0.560256 | 3.1:-70240463 | MS.gene79220:CDS |
| TAACCAACAAAGCCTAGTGT+TGG | 0.564525 | 3.1:-70240486 | MS.gene79220:CDS |
| TTGAAGATATATTTAGTGAG+TGG | 0.575240 | 3.1:-70240362 | MS.gene79220:CDS |
| TTGAATAATTGGAATGAGTG+TGG | 0.579695 | 3.1:-70240236 | MS.gene79220:CDS |
| GATTCTAGAATCACAAAACA+TGG | 0.587863 | 3.1:-70240399 | MS.gene79220:CDS |
| TAGTGTTGGAGCTAAGGCAA+AGG | 0.590191 | 3.1:-70240472 | MS.gene79220:CDS |
| CTCAACACCGGAAGTAGAAG+TGG | 0.592858 | 3.1:-70240315 | MS.gene79220:CDS |
| AGAGGAAATGTTCTCAACAC+CGG | 0.624098 | 3.1:-70240327 | MS.gene79220:CDS |
| GAGTGGAAGTGATTGCAAAG+AGG | 0.644927 | 3.1:-70240345 | MS.gene79220:CDS |
| TAAGGCAAAGGAAGATGTGG+GGG | 0.660678 | 3.1:-70240460 | MS.gene79220:CDS |
| AAACGTTGAATTCATCAAGT+CGG | 0.660973 | 3.1:-70240660 | MS.gene79220:CDS |
| CTAAGGCAAAGGAAGATGTG+GGG | 0.689053 | 3.1:-70240461 | MS.gene79220:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAACATGGAGTTGAAAAAA+TGG | - | chr3.1:70240488-70240507 | MS.gene79220:CDS | 25.0% |
| ! | TGGAAAAGAAAAATAAGAGT+AGG | - | chr3.1:70240614-70240633 | MS.gene79220:CDS | 25.0% |
| ! | TTGAAGATATATTTAGTGAG+TGG | - | chr3.1:70240511-70240530 | MS.gene79220:CDS | 25.0% |
| !! | ATAAGAGTAGGTTGAATAAT+TGG | - | chr3.1:70240626-70240645 | MS.gene79220:CDS | 25.0% |
| AAACGTTGAATTCATCAAGT+CGG | - | chr3.1:70240213-70240232 | MS.gene79220:CDS | 30.0% | |
| AGTGGAGATTAAAATAAGAG+TGG | - | chr3.1:70240576-70240595 | MS.gene79220:CDS | 30.0% | |
| GATTAAAATAAGAGTGGCTA+CGG | - | chr3.1:70240582-70240601 | MS.gene79220:CDS | 30.0% | |
| TTGAATAATTGGAATGAGTG+TGG | - | chr3.1:70240637-70240656 | MS.gene79220:CDS | 30.0% | |
| ! | AAGATTTTCCAAGAATTCCT+GGG | + | chr3.1:70240368-70240387 | None:intergenic | 30.0% |
| ! | TAAGATTTTCCAAGAATTCC+TGG | + | chr3.1:70240369-70240388 | None:intergenic | 30.0% |
| ! | TACCAAATCAGTACCATTTT+GGG | - | chr3.1:70240312-70240331 | MS.gene79220:CDS | 30.0% |
| !! | GATTCTAGAATCACAAAACA+TGG | - | chr3.1:70240474-70240493 | MS.gene79220:CDS | 30.0% |
| !!! | ATACTTTTGTTGTTTCCTGT+TGG | + | chr3.1:70240268-70240287 | None:intergenic | 30.0% |
| TTAAATGACTCGTCCCAAAA+TGG | + | chr3.1:70240328-70240347 | None:intergenic | 35.0% | |
| TTAATCTCCACTTCTACTTC+CGG | + | chr3.1:70240568-70240587 | None:intergenic | 35.0% | |
| ! | AGATTTTCCAAGAATTCCTG+GGG | + | chr3.1:70240367-70240386 | None:intergenic | 35.0% |
| ! | CTACCAAATCAGTACCATTT+TGG | - | chr3.1:70240311-70240330 | MS.gene79220:CDS | 35.0% |
| !! | GATTCTAGAATCACTTTCCA+TGG | + | chr3.1:70240466-70240485 | None:intergenic | 35.0% |
| AATGAGTGTGGATGCAAGAA+TGG | - | chr3.1:70240649-70240668 | MS.gene79220:CDS | 40.0% | |
| AGAGGAAATGTTCTCAACAC+CGG | - | chr3.1:70240546-70240565 | MS.gene79220:CDS | 40.0% | |
| CAACTGAAGTTTGCATATGC+AGG | + | chr3.1:70240169-70240188 | None:intergenic | 40.0% | |
| CGGCAAAAAACATGTTTGCA+AGG | - | chr3.1:70240233-70240252 | MS.gene79220:CDS | 40.0% | |
| GGGTCACAACTTATTGGAAA+AGG | - | chr3.1:70240433-70240452 | MS.gene79220:CDS | 40.0% | |
| TAACCAACAAAGCCTAGTGT+TGG | - | chr3.1:70240387-70240406 | MS.gene79220:CDS | 40.0% | |
| TTGGAAAAGGTGAGATTCCA+TGG | - | chr3.1:70240446-70240465 | MS.gene79220:CDS | 40.0% | |
| ! | GATTTTCCAAGAATTCCTGG+GGG | + | chr3.1:70240366-70240385 | None:intergenic | 40.0% |
| !! | GTCCCAAAATGGTACTGATT+TGG | + | chr3.1:70240317-70240336 | None:intergenic | 40.0% |
| AGCTAAGGCAAAGGAAGATG+TGG | - | chr3.1:70240410-70240429 | MS.gene79220:CDS | 45.0% | |
| AGTGGCTACGGTTCATGAAA+TGG | - | chr3.1:70240594-70240613 | MS.gene79220:CDS | 45.0% | |
| ATGTGGGGGTCACAACTTAT+TGG | - | chr3.1:70240427-70240446 | MS.gene79220:CDS | 45.0% | |
| CTAAGGCAAAGGAAGATGTG+GGG | - | chr3.1:70240412-70240431 | MS.gene79220:CDS | 45.0% | |
| GAGTGGAAGTGATTGCAAAG+AGG | - | chr3.1:70240528-70240547 | MS.gene79220:CDS | 45.0% | |
| GCTAAGGCAAAGGAAGATGT+GGG | - | chr3.1:70240411-70240430 | MS.gene79220:CDS | 45.0% | |
| TAAGGCAAAGGAAGATGTGG+GGG | - | chr3.1:70240413-70240432 | MS.gene79220:CDS | 45.0% | |
| TTTGCCTTAGCTCCAACACT+AGG | + | chr3.1:70240402-70240421 | None:intergenic | 45.0% | |
| ! | AAAGCCTAGTGTTGGAGCTA+AGG | - | chr3.1:70240395-70240414 | MS.gene79220:CDS | 45.0% |
| ! | GCATATGCAGGTGTTTTCGA+TGG | + | chr3.1:70240157-70240176 | None:intergenic | 45.0% |
| ! | TAGTGTTGGAGCTAAGGCAA+AGG | - | chr3.1:70240401-70240420 | MS.gene79220:CDS | 45.0% |
| ATTTGCGCTTAGCGCAGCTT+TGG | - | chr3.1:70240714-70240733 | MS.gene79220:CDS | 50.0% | |
| CTCAACACCGGAAGTAGAAG+TGG | - | chr3.1:70240558-70240577 | MS.gene79220:CDS | 50.0% | |
| GCAAGGCTGTATCTTCCAAC+AGG | - | chr3.1:70240250-70240269 | MS.gene79220:CDS | 50.0% | |
| TCTAGATTGTTCTTGCCCCC+AGG | - | chr3.1:70240348-70240367 | MS.gene79220:CDS | 50.0% | |
| TTCTTGCCCCCAGGAATTCT+TGG | - | chr3.1:70240357-70240376 | MS.gene79220:CDS | 50.0% | |
| ! | GCTCCAACACTAGGCTTTGT+TGG | + | chr3.1:70240393-70240412 | None:intergenic | 50.0% |
| ATGGCCATGATCATCGCGCT+TGG | - | chr3.1:70240668-70240687 | MS.gene79220:CDS | 55.0% | |
| TGCACCAAGCGCGATGATCA+TGG | + | chr3.1:70240675-70240694 | None:intergenic | 55.0% | |
| ! | TGCGCTTAGCGCAGCTTTGG+AGG | - | chr3.1:70240717-70240736 | MS.gene79220:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 70240148 | 70240747 | 70240148 | ID=MS.gene79220 |
| chr3.1 | mRNA | 70240148 | 70240747 | 70240148 | ID=MS.gene79220.t1;Parent=MS.gene79220 |
| chr3.1 | exon | 70240148 | 70240747 | 70240148 | ID=MS.gene79220.t1.exon1;Parent=MS.gene79220.t1 |
| chr3.1 | CDS | 70240148 | 70240747 | 70240148 | ID=cds.MS.gene79220.t1;Parent=MS.gene79220.t1 |
| Gene Sequence |
| Protein sequence |
