Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80205.t1 | XP_003603315.1 | 96.7 | 210 | 5 | 2 | 1 | 209 | 138 | 346 | 1.40E-98 | 369 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80205.t1 | G7J9W6 | 96.7 | 210 | 5 | 2 | 1 | 209 | 138 | 346 | 1.0e-98 | 369.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene80205 | 0.813131 | 2.99E-51 | -1.69E-46 |
| MS.gene04917 | MS.gene80205 | 0.818929 | 1.52E-52 | -1.69E-46 |
| MS.gene04998 | MS.gene80205 | 0.830655 | 2.60E-55 | -1.69E-46 |
| MS.gene050414 | MS.gene80205 | 0.805031 | 1.62E-49 | -1.69E-46 |
| MS.gene051852 | MS.gene80205 | 0.832719 | 8.05E-56 | -1.69E-46 |
| MS.gene052016 | MS.gene80205 | 0.855635 | 5.53E-62 | -1.69E-46 |
| MS.gene052463 | MS.gene80205 | 0.820953 | 5.22E-53 | -1.69E-46 |
| MS.gene052493 | MS.gene80205 | 0.810116 | 1.35E-50 | -1.69E-46 |
| MS.gene053786 | MS.gene80205 | 0.80486 | 1.76E-49 | -1.69E-46 |
| MS.gene054178 | MS.gene80205 | 0.826046 | 3.36E-54 | -1.69E-46 |
| MS.gene054617 | MS.gene80205 | 0.820266 | 7.51E-53 | -1.69E-46 |
| MS.gene054892 | MS.gene80205 | 0.809986 | 1.44E-50 | -1.69E-46 |
| MS.gene054893 | MS.gene80205 | 0.808892 | 2.48E-50 | -1.69E-46 |
| MS.gene055469 | MS.gene80205 | 0.808096 | 3.66E-50 | -1.69E-46 |
| MS.gene055513 | MS.gene80205 | 0.814382 | 1.58E-51 | -1.69E-46 |
| MS.gene055864 | MS.gene80205 | 0.809762 | 1.61E-50 | -1.69E-46 |
| MS.gene056285 | MS.gene80205 | 0.819614 | 1.06E-52 | -1.69E-46 |
| MS.gene056390 | MS.gene80205 | 0.80282 | 4.68E-49 | -1.69E-46 |
| MS.gene058429 | MS.gene80205 | 0.805006 | 1.64E-49 | -1.69E-46 |
| MS.gene058720 | MS.gene80205 | 0.85075 | 1.39E-60 | -1.69E-46 |
| MS.gene058758 | MS.gene80205 | 0.805102 | 1.57E-49 | -1.69E-46 |
| MS.gene059170 | MS.gene80205 | 0.843724 | 1.17E-58 | -1.69E-46 |
| MS.gene059774 | MS.gene80205 | 0.800044 | 1.73E-48 | -1.69E-46 |
| MS.gene060122 | MS.gene80205 | -0.800782 | 1.23E-48 | -1.69E-46 |
| MS.gene060259 | MS.gene80205 | 0.820742 | 5.84E-53 | -1.69E-46 |
| MS.gene06052 | MS.gene80205 | 0.802968 | 4.36E-49 | -1.69E-46 |
| MS.gene060697 | MS.gene80205 | 0.801723 | 7.87E-49 | -1.69E-46 |
| MS.gene060932 | MS.gene80205 | 0.840036 | 1.11E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80205.t1 | MTR_3g106250 | 98.095 | 210 | 2 | 2 | 1 | 209 | 138 | 346 | 7.08e-149 | 417 |
| MS.gene80205.t1 | MTR_1g013100 | 47.805 | 205 | 71 | 5 | 20 | 209 | 143 | 326 | 1.35e-46 | 156 |
| MS.gene80205.t1 | MTR_7g095100 | 32.273 | 220 | 93 | 8 | 1 | 208 | 130 | 305 | 1.02e-26 | 104 |
| MS.gene80205.t1 | MTR_1g081260 | 40.580 | 138 | 74 | 4 | 1 | 132 | 130 | 265 | 3.52e-26 | 102 |
| MS.gene80205.t1 | MTR_7g114790 | 31.193 | 218 | 100 | 7 | 1 | 208 | 127 | 304 | 5.44e-26 | 102 |
| MS.gene80205.t1 | MTR_5g069570 | 36.806 | 144 | 85 | 3 | 1 | 139 | 136 | 278 | 3.17e-24 | 97.4 |
| MS.gene80205.t1 | MTR_4g104760 | 39.130 | 138 | 64 | 5 | 1 | 122 | 133 | 266 | 1.35e-23 | 95.9 |
| MS.gene80205.t1 | MTR_3g082430 | 37.324 | 142 | 83 | 3 | 1 | 137 | 120 | 260 | 4.22e-23 | 94.4 |
| MS.gene80205.t1 | MTR_5g096570 | 40.323 | 124 | 69 | 2 | 3 | 122 | 1294 | 1416 | 1.05e-21 | 92.8 |
| MS.gene80205.t1 | MTR_3g076610 | 32.432 | 148 | 70 | 4 | 1 | 122 | 131 | 274 | 4.60e-19 | 84.0 |
| MS.gene80205.t1 | MTR_7g103900 | 32.576 | 132 | 79 | 4 | 1 | 122 | 142 | 273 | 2.58e-18 | 81.6 |
| MS.gene80205.t1 | MTR_4g055230 | 30.769 | 156 | 80 | 7 | 3 | 138 | 144 | 291 | 4.10e-17 | 78.2 |
| MS.gene80205.t1 | MTR_2g084965 | 34.375 | 128 | 74 | 5 | 3 | 122 | 149 | 274 | 4.59e-15 | 72.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80205.t1 | AT5G11000 | 56.552 | 145 | 56 | 2 | 1 | 138 | 144 | 288 | 1.24e-48 | 163 |
| MS.gene80205.t1 | AT2G25200 | 38.000 | 200 | 103 | 7 | 19 | 209 | 167 | 354 | 4.18e-29 | 111 |
| MS.gene80205.t1 | AT4G12690 | 38.971 | 136 | 79 | 2 | 1 | 133 | 126 | 260 | 2.71e-26 | 102 |
| MS.gene80205.t1 | AT4G12690 | 38.971 | 136 | 79 | 2 | 1 | 133 | 126 | 260 | 2.71e-26 | 102 |
| MS.gene80205.t1 | AT2G04220 | 38.806 | 134 | 77 | 2 | 1 | 130 | 130 | 262 | 9.45e-26 | 101 |
| MS.gene80205.t1 | AT5G28150 | 36.800 | 125 | 75 | 3 | 1 | 122 | 129 | 252 | 1.19e-23 | 95.9 |
| MS.gene80205.t1 | AT3G04860 | 27.830 | 212 | 96 | 5 | 1 | 208 | 129 | 287 | 1.47e-23 | 95.5 |
| MS.gene80205.t1 | AT5G48270 | 33.333 | 126 | 80 | 2 | 1 | 122 | 144 | 269 | 3.66e-20 | 87.0 |
| MS.gene80205.t1 | AT3G13229 | 36.220 | 127 | 73 | 4 | 3 | 122 | 112 | 237 | 2.24e-18 | 81.6 |
| MS.gene80205.t1 | AT2G27770 | 34.328 | 134 | 76 | 4 | 1 | 122 | 151 | 284 | 2.74e-18 | 81.6 |
Find 44 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTTATGAGTTAGGGAAAA+TGG | 0.148677 | 3.4:-92713919 | MS.gene80205:CDS |
| GGCGCAACAGAATTTGAATT+TGG | 0.231211 | 3.4:-92713943 | MS.gene80205:CDS |
| GCTTCTTCTGGTGGATCTTT+TGG | 0.246906 | 3.4:-92713864 | MS.gene80205:CDS |
| TGGCGCAAGTTCTTCTGTTT+TGG | 0.250517 | 3.4:-92713844 | MS.gene80205:CDS |
| TCTCATGGGATGTTTATAAT+TGG | 0.252772 | 3.4:-92714079 | MS.gene80205:CDS |
| AATTTGGGTGTTTATGAGTT+AGG | 0.282260 | 3.4:-92713927 | MS.gene80205:CDS |
| ATTCTTGTGTTCTTTACATT+TGG | 0.286420 | 3.4:+92714328 | None:intergenic |
| GTCGTCTTCGTCGGTTTCAA+TGG | 0.333982 | 3.4:-92713886 | MS.gene80205:CDS |
| TTTCTTTGCTTGTTTATGCT+TGG | 0.342405 | 3.4:-92713776 | MS.gene80205:CDS |
| TCGGTTTCAATGGCTTCTTC+TGG | 0.346131 | 3.4:-92713876 | MS.gene80205:CDS |
| GAAGAGTTTGACGAATTTGT+TGG | 0.356182 | 3.4:-92713964 | MS.gene80205:CDS |
| AGGCTCTGTTTCAGTGTAGA+TGG | 0.358342 | 3.4:-92714185 | MS.gene80205:CDS |
| AGATTGAAATGGAAGTTTAG+AGG | 0.395642 | 3.4:-92714140 | MS.gene80205:CDS |
| GCGCAACAGAATTTGAATTT+GGG | 0.399492 | 3.4:-92713942 | MS.gene80205:CDS |
| GTGGTGGAAGAGATCATTCC+AGG | 0.414862 | 3.4:-92714205 | MS.gene80205:CDS |
| ATTTGGGTGTTTATGAGTTA+GGG | 0.416856 | 3.4:-92713926 | MS.gene80205:CDS |
| GTTTATGAGTTAGGGAAAAT+GGG | 0.419124 | 3.4:-92713918 | MS.gene80205:CDS |
| ATGAATTGGTTGTTCCTGTT+GGG | 0.435531 | 3.4:-92713800 | MS.gene80205:CDS |
| TGATAACGAGATGACCCTTT+TGG | 0.440851 | 3.4:-92714384 | None:intergenic |
| GATTGAAATGGAAGTTTAGA+GGG | 0.459604 | 3.4:-92714139 | MS.gene80205:CDS |
| TACAACACGAGAGCGAGATT+TGG | 0.461614 | 3.4:-92714257 | MS.gene80205:CDS |
| TAAGAGTTGGTCGTCTTCGT+CGG | 0.462413 | 3.4:-92713895 | MS.gene80205:CDS |
| TGTTTGAATCTCCAACCAAA+AGG | 0.465336 | 3.4:+92714369 | None:intergenic |
| GTTTCAATGGCTTCTTCTGG+TGG | 0.473192 | 3.4:-92713873 | MS.gene80205:CDS |
| AATGAGAGAGTTGAAATTGA+TGG | 0.474861 | 3.4:-92714116 | MS.gene80205:CDS |
| AATGAATTGGTTGTTCCTGT+TGG | 0.475517 | 3.4:-92713801 | MS.gene80205:CDS |
| TGCAGATTAAGAGATTGAAA+TGG | 0.481189 | 3.4:-92714151 | MS.gene80205:CDS |
| GTGTACCTGTTCAGATCTCA+TGG | 0.482867 | 3.4:-92714094 | MS.gene80205:CDS |
| TAGTGTTGAGGAGAATGAAT+TGG | 0.486282 | 3.4:-92713814 | MS.gene80205:CDS |
| ATCTACACTGAAACAGAGCC+TGG | 0.491102 | 3.4:+92714187 | None:intergenic |
| ACATCCCATGAGATCTGAAC+AGG | 0.494374 | 3.4:+92714089 | None:intergenic |
| ACAAGCAAAGAAAACCCAAC+AGG | 0.504820 | 3.4:+92713786 | None:intergenic |
| AACACGAGAGCGAGATTTGG+TGG | 0.511269 | 3.4:-92714254 | MS.gene80205:CDS |
| GTTTGAATCTCCAACCAAAA+GGG | 0.512706 | 3.4:+92714370 | None:intergenic |
| AGAGAGATTCAGATAGATTG+TGG | 0.525407 | 3.4:-92714224 | MS.gene80205:CDS |
| GAGATTCAGATAGATTGTGG+TGG | 0.561448 | 3.4:-92714221 | MS.gene80205:CDS |
| TAGGGAAAATGGGTAAGAGT+TGG | 0.575570 | 3.4:-92713908 | MS.gene80205:CDS |
| TTTGGAATGGTCTAGTGTTG+AGG | 0.588570 | 3.4:-92713826 | MS.gene80205:CDS |
| TTGTGAAAGATAACAGTGAT+GGG | 0.630344 | 3.4:-92714052 | MS.gene80205:CDS |
| TTTGTGAAAGATAACAGTGA+TGG | 0.632305 | 3.4:-92714053 | MS.gene80205:CDS |
| ACACGAGAGCGAGATTTGGT+GGG | 0.635309 | 3.4:-92714253 | MS.gene80205:CDS |
| TGTACCTGTTCAGATCTCAT+GGG | 0.704348 | 3.4:-92714093 | MS.gene80205:CDS |
| GAAGATGAAGATGAACAACA+AGG | 0.707918 | 3.4:-92713996 | MS.gene80205:CDS |
| GATGAAGATGAACAACAAGG+AGG | 0.746013 | 3.4:-92713993 | MS.gene80205:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTCTTGTGTTCTTTACATT+TGG | + | chr3.4:92713816-92713835 | None:intergenic | 25.0% |
| !! | ACACAAGAATTTTCAGTATT+TGG | - | chr3.4:92713826-92713845 | MS.gene80205:CDS | 25.0% |
| AATGAGAGAGTTGAAATTGA+TGG | - | chr3.4:92714025-92714044 | MS.gene80205:CDS | 30.0% | |
| AATTTGGGTGTTTATGAGTT+AGG | - | chr3.4:92714214-92714233 | MS.gene80205:CDS | 30.0% | |
| AGATTGAAATGGAAGTTTAG+AGG | - | chr3.4:92714001-92714020 | MS.gene80205:CDS | 30.0% | |
| ATTTGGGTGTTTATGAGTTA+GGG | - | chr3.4:92714215-92714234 | MS.gene80205:CDS | 30.0% | |
| GATTGAAATGGAAGTTTAGA+GGG | - | chr3.4:92714002-92714021 | MS.gene80205:CDS | 30.0% | |
| GTTTATGAGTTAGGGAAAAT+GGG | - | chr3.4:92714223-92714242 | MS.gene80205:CDS | 30.0% | |
| TCTCATGGGATGTTTATAAT+TGG | - | chr3.4:92714062-92714081 | MS.gene80205:CDS | 30.0% | |
| TGCAGATTAAGAGATTGAAA+TGG | - | chr3.4:92713990-92714009 | MS.gene80205:CDS | 30.0% | |
| TGTTTATGAGTTAGGGAAAA+TGG | - | chr3.4:92714222-92714241 | MS.gene80205:CDS | 30.0% | |
| TTGTGAAAGATAACAGTGAT+GGG | - | chr3.4:92714089-92714108 | MS.gene80205:CDS | 30.0% | |
| TTTCTTTGCTTGTTTATGCT+TGG | - | chr3.4:92714365-92714384 | MS.gene80205:CDS | 30.0% | |
| TTTGTGAAAGATAACAGTGA+TGG | - | chr3.4:92714088-92714107 | MS.gene80205:CDS | 30.0% | |
| AATGAATTGGTTGTTCCTGT+TGG | - | chr3.4:92714340-92714359 | MS.gene80205:CDS | 35.0% | |
| AGAGAGATTCAGATAGATTG+TGG | - | chr3.4:92713917-92713936 | MS.gene80205:CDS | 35.0% | |
| ATGAATTGGTTGTTCCTGTT+GGG | - | chr3.4:92714341-92714360 | MS.gene80205:CDS | 35.0% | |
| GAAGAGTTTGACGAATTTGT+TGG | - | chr3.4:92714177-92714196 | MS.gene80205:CDS | 35.0% | |
| GAAGATGAAGATGAACAACA+AGG | - | chr3.4:92714145-92714164 | MS.gene80205:CDS | 35.0% | |
| GCGCAACAGAATTTGAATTT+GGG | - | chr3.4:92714199-92714218 | MS.gene80205:CDS | 35.0% | |
| GTTTGAATCTCCAACCAAAA+GGG | + | chr3.4:92713774-92713793 | None:intergenic | 35.0% | |
| TGTTTGAATCTCCAACCAAA+AGG | + | chr3.4:92713775-92713794 | None:intergenic | 35.0% | |
| ! | TAGTGTTGAGGAGAATGAAT+TGG | - | chr3.4:92714327-92714346 | MS.gene80205:CDS | 35.0% |
| !!! | CAAGTTCTTCTGTTTTGGAA+TGG | - | chr3.4:92714302-92714321 | MS.gene80205:CDS | 35.0% |
| ACAAGCAAAGAAAACCCAAC+AGG | + | chr3.4:92714358-92714377 | None:intergenic | 40.0% | |
| GAGATTCAGATAGATTGTGG+TGG | - | chr3.4:92713920-92713939 | MS.gene80205:CDS | 40.0% | |
| GATGAAGATGAACAACAAGG+AGG | - | chr3.4:92714148-92714167 | MS.gene80205:CDS | 40.0% | |
| GGCGCAACAGAATTTGAATT+TGG | - | chr3.4:92714198-92714217 | MS.gene80205:CDS | 40.0% | |
| TAGGGAAAATGGGTAAGAGT+TGG | - | chr3.4:92714233-92714252 | MS.gene80205:CDS | 40.0% | |
| TGTACCTGTTCAGATCTCAT+GGG | - | chr3.4:92714048-92714067 | MS.gene80205:CDS | 40.0% | |
| ! | TTTGGAATGGTCTAGTGTTG+AGG | - | chr3.4:92714315-92714334 | MS.gene80205:CDS | 40.0% |
| ACATCCCATGAGATCTGAAC+AGG | + | chr3.4:92714055-92714074 | None:intergenic | 45.0% | |
| AGGCTCTGTTTCAGTGTAGA+TGG | - | chr3.4:92713956-92713975 | MS.gene80205:CDS | 45.0% | |
| ATCTACACTGAAACAGAGCC+TGG | + | chr3.4:92713957-92713976 | None:intergenic | 45.0% | |
| GTGTACCTGTTCAGATCTCA+TGG | - | chr3.4:92714047-92714066 | MS.gene80205:CDS | 45.0% | |
| GTTTCAATGGCTTCTTCTGG+TGG | - | chr3.4:92714268-92714287 | MS.gene80205:CDS | 45.0% | |
| TACAACACGAGAGCGAGATT+TGG | - | chr3.4:92713884-92713903 | MS.gene80205:CDS | 45.0% | |
| TCGGTTTCAATGGCTTCTTC+TGG | - | chr3.4:92714265-92714284 | MS.gene80205:CDS | 45.0% | |
| ! | TAAGAGTTGGTCGTCTTCGT+CGG | - | chr3.4:92714246-92714265 | MS.gene80205:CDS | 45.0% |
| ! | TGGCGCAAGTTCTTCTGTTT+TGG | - | chr3.4:92714297-92714316 | MS.gene80205:CDS | 45.0% |
| !! | GCTTCTTCTGGTGGATCTTT+TGG | - | chr3.4:92714277-92714296 | MS.gene80205:CDS | 45.0% |
| AACACGAGAGCGAGATTTGG+TGG | - | chr3.4:92713887-92713906 | MS.gene80205:CDS | 50.0% | |
| ! | ACACGAGAGCGAGATTTGGT+GGG | - | chr3.4:92713888-92713907 | MS.gene80205:CDS | 50.0% |
| ! | GTGGTGGAAGAGATCATTCC+AGG | - | chr3.4:92713936-92713955 | MS.gene80205:CDS | 50.0% |
| !! | GTCGTCTTCGTCGGTTTCAA+TGG | - | chr3.4:92714255-92714274 | MS.gene80205:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 92713767 | 92714396 | 92713767 | ID=MS.gene80205 |
| chr3.4 | mRNA | 92713767 | 92714396 | 92713767 | ID=MS.gene80205.t1;Parent=MS.gene80205 |
| chr3.4 | exon | 92713767 | 92714396 | 92713767 | ID=MS.gene80205.t1.exon1;Parent=MS.gene80205.t1 |
| chr3.4 | CDS | 92713767 | 92714396 | 92713767 | ID=cds.MS.gene80205.t1;Parent=MS.gene80205.t1 |
| Gene Sequence |
| Protein sequence |
