Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81108.t1 | XP_013464148.1 | 100 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 9.10E-33 | 149.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81108.t1 | I3SDA0 | 100.0 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 6.6e-33 | 149.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049181 | MS.gene81108 | 0.836047 | 1.18E-56 | -1.69E-46 |
| MS.gene049281 | MS.gene81108 | 0.824687 | 7.05E-54 | -1.69E-46 |
| MS.gene049313 | MS.gene81108 | 0.821178 | 4.63E-53 | -1.69E-46 |
| MS.gene050363 | MS.gene81108 | 0.853502 | 2.29E-61 | -1.69E-46 |
| MS.gene050381 | MS.gene81108 | 0.802689 | 4.98E-49 | -1.69E-46 |
| MS.gene050409 | MS.gene81108 | 0.803651 | 3.15E-49 | -1.69E-46 |
| MS.gene050412 | MS.gene81108 | 0.802694 | 4.97E-49 | -1.69E-46 |
| MS.gene050416 | MS.gene81108 | 0.823682 | 1.21E-53 | -1.69E-46 |
| MS.gene050883 | MS.gene81108 | 0.831423 | 1.68E-55 | -1.69E-46 |
| MS.gene051946 | MS.gene81108 | 0.820812 | 5.63E-53 | -1.69E-46 |
| MS.gene052091 | MS.gene81108 | 0.843365 | 1.47E-58 | -1.69E-46 |
| MS.gene052482 | MS.gene81108 | -0.809414 | 1.91E-50 | -1.69E-46 |
| MS.gene052486 | MS.gene81108 | -0.815677 | 8.18E-52 | -1.69E-46 |
| MS.gene053549 | MS.gene81108 | 0.83258 | 8.72E-56 | -1.69E-46 |
| MS.gene053826 | MS.gene81108 | 0.844532 | 7.13E-59 | -1.69E-46 |
| MS.gene053932 | MS.gene81108 | 0.810899 | 9.16E-51 | -1.69E-46 |
| MS.gene055549 | MS.gene81108 | 0.873287 | 1.65E-67 | -1.69E-46 |
| MS.gene055550 | MS.gene81108 | 0.877826 | 4.60E-69 | -1.69E-46 |
| MS.gene055551 | MS.gene81108 | 0.830093 | 3.57E-55 | -1.69E-46 |
| MS.gene056311 | MS.gene81108 | 0.834774 | 2.47E-56 | -1.69E-46 |
| MS.gene056408 | MS.gene81108 | 0.850664 | 1.47E-60 | -1.69E-46 |
| MS.gene056530 | MS.gene81108 | 0.807485 | 4.94E-50 | -1.69E-46 |
| MS.gene057014 | MS.gene81108 | 0.823942 | 1.06E-53 | -1.69E-46 |
| MS.gene058493 | MS.gene81108 | 0.819387 | 1.19E-52 | -1.69E-46 |
| MS.gene058494 | MS.gene81108 | 0.819665 | 1.03E-52 | -1.69E-46 |
| MS.gene058628 | MS.gene81108 | 0.810802 | 9.62E-51 | -1.69E-46 |
| MS.gene058630 | MS.gene81108 | 0.807257 | 5.52E-50 | -1.69E-46 |
| MS.gene060237 | MS.gene81108 | 0.802029 | 6.81E-49 | -1.69E-46 |
| MS.gene060703 | MS.gene81108 | -0.802231 | 6.19E-49 | -1.69E-46 |
| MS.gene06077 | MS.gene81108 | -0.800012 | 1.76E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81108.t1 | MTR_2g061780 | 100.000 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 1.36e-53 | 161 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81108.t1 | AT2G31945 | 60.938 | 64 | 23 | 1 | 1 | 62 | 1 | 64 | 1.78e-20 | 78.2 |
| MS.gene81108.t1 | AT1G05575 | 56.000 | 75 | 31 | 1 | 1 | 73 | 1 | 75 | 2.14e-17 | 70.1 |
Find 20 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTGATCTCTTGATGTTCT+TGG | 0.211211 | 2.3:-32378552 | None:intergenic |
| GAAGGAGAGGGTTCTCCTTC+AGG | 0.307513 | 2.3:-32378638 | None:intergenic |
| TTGATGAATTTGAAAGGATA+AGG | 0.383924 | 2.3:-32378520 | None:intergenic |
| CTCTGAGTTGCTTGTGATTG+AGG | 0.403222 | 2.3:-32378671 | None:intergenic |
| GATTGAGGACTCATTGTAGA+AGG | 0.403857 | 2.3:-32378656 | None:intergenic |
| CGTGAGTATTCGTCGGATGT+TGG | 0.440469 | 2.3:+32378595 | MS.gene81108:CDS |
| CAAAAGATGGATAGTAGCTT+AGG | 0.451025 | 2.3:+32378446 | None:intergenic |
| CTTAGGTGACATGTTATTGA+AGG | 0.471923 | 2.3:+32378463 | MS.gene81108:CDS |
| GTTCGTCCTAGTCCAAGCTT+TGG | 0.507181 | 2.3:+32378493 | MS.gene81108:CDS |
| GGATAAGGTACACCAAAGCT+TGG | 0.548918 | 2.3:-32378505 | None:intergenic |
| CGCGTTCCGTGAGTATTCGT+CGG | 0.549054 | 2.3:+32378588 | MS.gene81108:CDS |
| ATATGTTTGATGAATTTGAA+AGG | 0.553082 | 2.3:-32378526 | None:intergenic |
| AGGTGACATGTTATTGAAGG+TGG | 0.573319 | 2.3:+32378466 | MS.gene81108:CDS |
| CGAATACTCACGGAACGCGC+CGG | 0.585547 | 2.3:-32378584 | None:intergenic |
| GAGATCAAACAGTTTCAAGC+CGG | 0.604551 | 2.3:+32378565 | MS.gene81108:CDS |
| AGGACTCATTGTAGAAGGAG+AGG | 0.608887 | 2.3:-32378651 | None:intergenic |
| GGTACACCAAAGCTTGGACT+AGG | 0.611822 | 2.3:-32378499 | None:intergenic |
| GGACTCATTGTAGAAGGAGA+GGG | 0.615228 | 2.3:-32378650 | None:intergenic |
| GGAGAGGGTTCTCCTTCAGG+AGG | 0.644566 | 2.3:-32378635 | None:intergenic |
| CAACATCCGACGAATACTCA+CGG | 0.680704 | 2.3:-32378594 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATGTTTGATGAATTTGAA+AGG | - | chr2.3:32378529-32378548 | None:intergenic | 20.0% |
| ! | TTGATGAATTTGAAAGGATA+AGG | - | chr2.3:32378523-32378542 | None:intergenic | 25.0% |
| CTTAGGTGACATGTTATTGA+AGG | + | chr2.3:32378463-32378482 | MS.gene81108:CDS | 35.0% | |
| GTTTGATCTCTTGATGTTCT+TGG | - | chr2.3:32378555-32378574 | None:intergenic | 35.0% | |
| AGGTGACATGTTATTGAAGG+TGG | + | chr2.3:32378466-32378485 | MS.gene81108:CDS | 40.0% | |
| GAGATCAAACAGTTTCAAGC+CGG | + | chr2.3:32378565-32378584 | MS.gene81108:CDS | 40.0% | |
| GATTGAGGACTCATTGTAGA+AGG | - | chr2.3:32378659-32378678 | None:intergenic | 40.0% | |
| AGGACTCATTGTAGAAGGAG+AGG | - | chr2.3:32378654-32378673 | None:intergenic | 45.0% | |
| CAACATCCGACGAATACTCA+CGG | - | chr2.3:32378597-32378616 | None:intergenic | 45.0% | |
| CTCTGAGTTGCTTGTGATTG+AGG | - | chr2.3:32378674-32378693 | None:intergenic | 45.0% | |
| GGACTCATTGTAGAAGGAGA+GGG | - | chr2.3:32378653-32378672 | None:intergenic | 45.0% | |
| GGATAAGGTACACCAAAGCT+TGG | - | chr2.3:32378508-32378527 | None:intergenic | 45.0% | |
| ! | CTCTCTGATTTTCCTCCTGA+AGG | + | chr2.3:32378623-32378642 | MS.gene81108:CDS | 45.0% |
| CGTGAGTATTCGTCGGATGT+TGG | + | chr2.3:32378595-32378614 | MS.gene81108:CDS | 50.0% | |
| GGTACACCAAAGCTTGGACT+AGG | - | chr2.3:32378502-32378521 | None:intergenic | 50.0% | |
| GTTCGTCCTAGTCCAAGCTT+TGG | + | chr2.3:32378493-32378512 | MS.gene81108:CDS | 50.0% | |
| CGCGTTCCGTGAGTATTCGT+CGG | + | chr2.3:32378588-32378607 | MS.gene81108:CDS | 55.0% | |
| GAAGGAGAGGGTTCTCCTTC+AGG | - | chr2.3:32378641-32378660 | None:intergenic | 55.0% | |
| CGAATACTCACGGAACGCGC+CGG | - | chr2.3:32378587-32378606 | None:intergenic | 60.0% | |
| GGAGAGGGTTCTCCTTCAGG+AGG | - | chr2.3:32378638-32378657 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 32378452 | 32378697 | 32378452 | ID=MS.gene81108 |
| chr2.3 | mRNA | 32378452 | 32378697 | 32378452 | ID=MS.gene81108.t1;Parent=MS.gene81108 |
| chr2.3 | exon | 32378452 | 32378697 | 32378452 | ID=MS.gene81108.t1.exon1;Parent=MS.gene81108.t1 |
| chr2.3 | CDS | 32378452 | 32378697 | 32378452 | ID=cds.MS.gene81108.t1;Parent=MS.gene81108.t1 |
| Gene Sequence |
| Protein sequence |
