AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81771


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81771.t1 MTR_1g058560 98.462 195 3 0 1 195 1 195 6.17e-138 383
MS.gene81771.t1 MTR_1g058540 81.633 196 35 1 1 195 1 196 6.87e-100 287
MS.gene81771.t1 MTR_1g058570 88.205 195 22 1 1 195 1 194 1.32e-99 286
MS.gene81771.t1 MTR_2g086740 60.694 173 68 0 22 194 47 219 7.52e-59 184
MS.gene81771.t1 MTR_7g011090 52.941 170 80 0 26 195 17 186 4.19e-56 176
MS.gene81771.t1 MTR_4g081880 42.932 191 103 2 4 194 2 186 6.88e-51 162
MS.gene81771.t1 MTR_5g041900 48.503 167 86 0 29 195 20 186 5.07e-45 147
MS.gene81771.t1 MTR_2g086740 63.462 104 38 0 22 125 47 150 2.23e-34 119
MS.gene81771.t1 MTR_0004s0210 37.419 155 84 4 36 188 7 150 5.21e-21 85.5
MS.gene81771.t1 MTR_0097s0070 33.898 118 76 2 72 188 69 185 6.44e-14 67.4
MS.gene81771.t1 MTR_4g118800 30.833 120 82 1 74 192 62 181 1.86e-12 63.2
MS.gene81771.t1 MTR_8g089300 29.487 156 101 3 41 188 30 184 1.90e-12 63.5
MS.gene81771.t1 MTR_7g108130 27.742 155 109 2 36 188 28 181 2.47e-12 63.2
MS.gene81771.t1 MTR_3g064310 32.203 118 77 2 75 192 77 191 3.08e-12 63.2
MS.gene81771.t1 MTR_5g076480 34.483 116 72 3 75 189 69 181 4.25e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81771.t1 AT2G36100 56.863 204 76 3 2 195 5 206 1.27e-70 213
MS.gene81771.t1 AT3G11550 62.189 201 68 3 2 195 5 204 3.20e-64 197
MS.gene81771.t1 AT5G06200 61.616 198 70 3 2 194 5 201 1.02e-63 196
MS.gene81771.t1 AT2G27370 51.724 203 83 2 7 194 17 219 1.99e-62 193
MS.gene81771.t1 AT5G15290 44.828 174 86 1 2 175 3 166 1.91e-49 159
MS.gene81771.t1 AT1G14160 41.212 165 97 0 31 195 45 209 3.99e-44 146
MS.gene81771.t1 AT1G03700 32.886 149 91 2 40 188 12 151 2.50e-19 81.3
MS.gene81771.t1 AT1G17200 27.660 188 124 4 9 192 2 181 2.00e-16 74.3
MS.gene81771.t1 AT4G03540 33.058 121 72 2 56 176 28 139 2.31e-15 70.9
MS.gene81771.t1 AT5G44550 33.951 162 95 4 41 194 22 179 2.68e-15 71.2
MS.gene81771.t1 AT3G06390 30.075 133 88 2 37 164 37 169 2.78e-13 65.9
MS.gene81771.t1 AT4G15630 26.601 203 118 6 2 192 6 189 7.74e-12 61.6
MS.gene81771.t1 AT4G15610 26.144 153 107 3 28 175 20 171 8.02e-12 61.6

Find 40 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCATCCTCCTGGCCTTGC+AGG 0.176057 1.3:+43254749 None:intergenic
CTTGCCATTTGCAACCAATT+TGG 0.187527 1.3:-43253753 MS.gene81771:CDS
TGATTCTTGAATATCAATTT+TGG 0.211847 1.3:+43254811 None:intergenic
GTGATGTCTGCCTTGGCTAT+TGG 0.255520 1.3:-43253657 MS.gene81771:CDS
GGCTATAGTGGCTGGCTATT+TGG 0.277845 1.3:-43254395 MS.gene81771:CDS
TGATGTCTGCCTTGGCTATT+GGG 0.286725 1.3:-43253656 MS.gene81771:CDS
CCTTAAAATGAAATCCATTA+TGG 0.301071 1.3:+43254715 None:intergenic
TACTAAAGGAAAAGCTGTTT+TGG 0.315060 1.3:-43254780 MS.gene81771:CDS
ATGATGGCTACTATAGAAAA+AGG 0.348696 1.3:+43254361 None:intergenic
AAGGCCAGGAGGATGGAAAA+AGG 0.353235 1.3:-43254744 MS.gene81771:CDS
TCTTATTGCAATGGCTATAG+TGG 0.369985 1.3:-43254407 MS.gene81771:CDS
TCTTGCCGCTGCCGCCTCGA+TGG 0.384071 1.3:-43254672 MS.gene81771:CDS
AGCTGCTTCCATAGTTTACT+TGG 0.385601 1.3:-43253805 MS.gene81771:CDS
CTTGCCGCTGCCGCCTCGAT+GGG 0.402133 1.3:-43254671 MS.gene81771:CDS
ATCATACGTCCCCATGCAAC+TGG 0.403133 1.3:-43254343 MS.gene81771:CDS
ATGGAAATCAAGACACAAAC+TGG 0.422312 1.3:-43253776 MS.gene81771:CDS
AGCGGCAAGAGCTGCTGCTA+TGG 0.432197 1.3:+43254685 None:intergenic
ATCTCCAAATTGGTTGCAAA+TGG 0.439108 1.3:+43253749 None:intergenic
GTTGGTTGTGATGTCTGCCT+TGG 0.449789 1.3:-43253664 MS.gene81771:CDS
GTTGCAGCACCTGCAAGGCC+AGG 0.457764 1.3:-43254758 MS.gene81771:CDS
CACCTGCAAGGCCAGGAGGA+TGG 0.475215 1.3:-43254751 MS.gene81771:CDS
ATTGCAGGTATTTCTTACTC+TGG 0.476475 1.3:-43253847 MS.gene81771:intron
AGTTGCATGGGGACGTATGA+TGG 0.492360 1.3:+43254345 None:intergenic
ATTGTGTGCCAAGTAAACTA+TGG 0.493367 1.3:+43253797 None:intergenic
AATGTTACATACTGGAATGC+AGG 0.494232 1.3:+43254581 None:intergenic
AAGCCTTGGTCCAGTTGCAT+GGG 0.503885 1.3:+43254333 None:intergenic
TGTTCCCATCGAGGCGGCAG+CGG 0.517932 1.3:+43254667 None:intergenic
ATAGAGAGCTTACGGTGTCT+AGG 0.520494 1.3:+43254299 None:intergenic
AAAGCCTTGGTCCAGTTGCA+TGG 0.525434 1.3:+43254332 None:intergenic
ATTGCAATGGCTATAGTGGC+TGG 0.542648 1.3:-43254403 MS.gene81771:CDS
GAATCAAGCAAAGATACTAA+AGG 0.552945 1.3:-43254794 MS.gene81771:CDS
ACAGACAAGTGGAGCAGTTG+TGG 0.555649 1.3:-43253718 MS.gene81771:CDS
TGTGGCATCATTTATTACTG+TGG 0.599611 1.3:-43253700 MS.gene81771:CDS
TCAATGCTTCCCAATAGCCA+AGG 0.611842 1.3:+43253647 None:intergenic
ATAGTTTACTTGGCACACAA+TGG 0.619739 1.3:-43253795 MS.gene81771:CDS
GCAGCACCTGCAAGGCCAGG+AGG 0.636977 1.3:-43254755 MS.gene81771:CDS
ATCACTTGTTCCCATCGAGG+CGG 0.653937 1.3:+43254661 None:intergenic
AGCCTTGGTCCAGTTGCATG+GGG 0.659858 1.3:+43254334 None:intergenic
TTGATCACTTGTTCCCATCG+AGG 0.661241 1.3:+43254658 None:intergenic
GTCCCCATGCAACTGGACCA+AGG 0.673252 1.3:-43254336 MS.gene81771:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTCTTAAAAAAAAAAAATT+AGG - chr1.3:43254531-43254550 MS.gene81771:intron 10.0%
!! TTAAAAAAAAAAAATTAGGT+GGG - chr1.3:43254535-43254554 MS.gene81771:intron 10.0%
!! CTTAAAAAAAAAAAATTAGG+TGG - chr1.3:43254534-43254553 MS.gene81771:intron 15.0%
!! CTCAAATATAAGTAAAAGTT+AGG - chr1.3:43254243-43254262 MS.gene81771:intron 20.0%
!!! GATGTATTTAATTCAACTTT+TGG + chr1.3:43254293-43254312 None:intergenic 20.0%
!!! TGATTCTTGAATATCAATTT+TGG + chr1.3:43253657-43253676 None:intergenic 20.0%
!!! TTAGTTATGATTTTTCTTTG+TGG - chr1.3:43254587-43254606 MS.gene81771:intron 20.0%
! CCTTAAAATGAAATCCATTA+TGG + chr1.3:43253753-43253772 None:intergenic 25.0%
! TTCTAAGTCACATATAAGAT+CGG - chr1.3:43253929-43253948 MS.gene81771:intron 25.0%
!!! ATGGATTTCATTTTAAGGTT+AGG - chr1.3:43253755-43253774 MS.gene81771:CDS 25.0%
!!! CCATAATGGATTTCATTTTA+AGG - chr1.3:43253750-43253769 MS.gene81771:CDS 25.0%
!!! CTTTTACTTATATTTGAGAC+TGG + chr1.3:43254241-43254260 None:intergenic 25.0%
!!! TTATGATTTTTCAATCCACT+TGG - chr1.3:43254410-43254429 MS.gene81771:CDS 25.0%
AAAAAAAAAATTAGGTGGGC+TGG - chr1.3:43254539-43254558 MS.gene81771:intron 30.0%
ACTTAATGCTTGAAAAATGC+AGG - chr1.3:43254030-43254049 MS.gene81771:intron 30.0%
ATGATGGCTACTATAGAAAA+AGG + chr1.3:43254107-43254126 None:intergenic 30.0%
GAATCAAGCAAAGATACTAA+AGG - chr1.3:43253671-43253690 MS.gene81771:CDS 30.0%
GTTAGAAAATAGAGAGCTTA+CGG + chr1.3:43254177-43254196 None:intergenic 30.0%
! TACTAAAGGAAAAGCTGTTT+TGG - chr1.3:43253685-43253704 MS.gene81771:CDS 30.0%
! TACTTATATTTGAGACTGGA+GGG + chr1.3:43254237-43254256 None:intergenic 30.0%
! TTACTTATATTTGAGACTGG+AGG + chr1.3:43254238-43254257 None:intergenic 30.0%
!! ATTTTTCAATCCACTTGGAT+TGG - chr1.3:43254415-43254434 MS.gene81771:CDS 30.0%
!!! CAGGTTTTTTCTTATTGCAA+TGG - chr1.3:43254049-43254068 MS.gene81771:intron 30.0%
!!! GAGTTTTGAATGTTACATAC+TGG + chr1.3:43253895-43253914 None:intergenic 30.0%
!!! TGTGGTTGTTTTAATCTTGT+TGG - chr1.3:43254783-43254802 MS.gene81771:CDS 30.0%
AATGTTACATACTGGAATGC+AGG + chr1.3:43253887-43253906 None:intergenic 35.0%
ATAGTTTACTTGGCACACAA+TGG - chr1.3:43254670-43254689 MS.gene81771:CDS 35.0%
ATCTCCAAATTGGTTGCAAA+TGG + chr1.3:43254719-43254738 None:intergenic 35.0%
ATGGAAATCAAGACACAAAC+TGG - chr1.3:43254689-43254708 MS.gene81771:CDS 35.0%
ATTGCAGGTATTTCTTACTC+TGG - chr1.3:43254618-43254637 MS.gene81771:CDS 35.0%
ATTGTGTGCCAAGTAAACTA+TGG + chr1.3:43254671-43254690 None:intergenic 35.0%
CTAACTCTCGATCATTTCTT+TGG - chr1.3:43254192-43254211 MS.gene81771:intron 35.0%
GTGCACAAAAATCTCCAAAT+TGG + chr1.3:43254729-43254748 None:intergenic 35.0%
TAGATGTTTACTACTCGCTT+CGG + chr1.3:43254352-43254371 None:intergenic 35.0%
TCTTATTGCAATGGCTATAG+TGG - chr1.3:43254058-43254077 MS.gene81771:intron 35.0%
TGTGGCATCATTTATTACTG+TGG - chr1.3:43254765-43254784 MS.gene81771:CDS 35.0%
TTTGTGGTGCTTATAATTGC+AGG - chr1.3:43254603-43254622 MS.gene81771:CDS 35.0%
! ACTTATATTTGAGACTGGAG+GGG + chr1.3:43254236-43254255 None:intergenic 35.0%
AGCTGCTTCCATAGTTTACT+TGG - chr1.3:43254660-43254679 MS.gene81771:CDS 40.0%
AGGATGATGAGAAAAAGCCT+TGG + chr1.3:43254149-43254168 None:intergenic 40.0%
ATTCTCTCCAGTGCCAATTT+AGG - chr1.3:43254495-43254514 MS.gene81771:intron 40.0%
TTAAACCAGCCCACCTAAAT+TGG + chr1.3:43254511-43254530 None:intergenic 40.0%
TTATGCTAATACTAGCGTGC+AGG + chr1.3:43254386-43254405 None:intergenic 40.0%
! CTTATATTTGAGACTGGAGG+GGG + chr1.3:43254235-43254254 None:intergenic 40.0%
! CTTGCCATTTGCAACCAATT+TGG - chr1.3:43254712-43254731 MS.gene81771:CDS 40.0%
! GAAAAAGGGCATAGCCATAA+TGG - chr1.3:43253736-43253755 MS.gene81771:CDS 40.0%
! GATTTTTGTGCACAGACAAG+TGG - chr1.3:43254736-43254755 MS.gene81771:CDS 40.0%
!! GTGGTATTGACTTGTGATCT+GGG - chr1.3:43254442-43254461 MS.gene81771:intron 40.0%
ATAGAGAGCTTACGGTGTCT+AGG + chr1.3:43254169-43254188 None:intergenic 45.0%
ATTGCAATGGCTATAGTGGC+TGG - chr1.3:43254062-43254081 MS.gene81771:intron 45.0%
GATCACAAGTCAATACCACC+AGG + chr1.3:43254441-43254460 None:intergenic 45.0%
TCAATGCTTCCCAATAGCCA+AGG + chr1.3:43254821-43254840 None:intergenic 45.0%
TCTCCAGTGCCAATTTAGGT+GGG - chr1.3:43254499-43254518 MS.gene81771:intron 45.0%
TGATGTCTGCCTTGGCTATT+GGG - chr1.3:43254809-43254828 MS.gene81771:CDS 45.0%
TTGATCACTTGTTCCCATCG+AGG + chr1.3:43253810-43253829 None:intergenic 45.0%
!! GGTGGTATTGACTTGTGATC+TGG - chr1.3:43254441-43254460 MS.gene81771:intron 45.0%
AAAGCCTTGGTCCAGTTGCA+TGG + chr1.3:43254136-43254155 None:intergenic 50.0%
AAGCCTTGGTCCAGTTGCAT+GGG + chr1.3:43254135-43254154 None:intergenic 50.0%
AAGGCCAGGAGGATGGAAAA+AGG - chr1.3:43253721-43253740 MS.gene81771:CDS 50.0%
ACAGACAAGTGGAGCAGTTG+TGG - chr1.3:43254747-43254766 MS.gene81771:CDS 50.0%
AGGCCAGGAGGATGGAAAAA+GGG - chr1.3:43253722-43253741 MS.gene81771:CDS 50.0%
AGTTGCATGGGGACGTATGA+TGG + chr1.3:43254123-43254142 None:intergenic 50.0%
ATCACTTGTTCCCATCGAGG+CGG + chr1.3:43253807-43253826 None:intergenic 50.0%
ATCATACGTCCCCATGCAAC+TGG - chr1.3:43254122-43254141 MS.gene81771:intron 50.0%
CTCTCCAGTGCCAATTTAGG+TGG - chr1.3:43254498-43254517 MS.gene81771:intron 50.0%
GTGATGTCTGCCTTGGCTAT+TGG - chr1.3:43254808-43254827 MS.gene81771:CDS 50.0%
GTTGGTTGTGATGTCTGCCT+TGG - chr1.3:43254801-43254820 MS.gene81771:CDS 50.0%
! ATGCCCTTTTTCCATCCTCC+TGG + chr1.3:43253728-43253747 None:intergenic 50.0%
! GGCTATAGTGGCTGGCTATT+TGG - chr1.3:43254070-43254089 MS.gene81771:intron 50.0%
!! TCAATCCACTTGGATTGGCC+TGG - chr1.3:43254420-43254439 MS.gene81771:intron 50.0%
!! TTTTGGTTGCAGCACCTGCA+AGG - chr1.3:43253702-43253721 MS.gene81771:CDS 50.0%
AGCCTTGGTCCAGTTGCATG+GGG + chr1.3:43254134-43254153 None:intergenic 55.0%
CAGCCCACCTAAATTGGCAC+TGG + chr1.3:43254505-43254524 None:intergenic 55.0%
CAGTGCCAATTTAGGTGGGC+TGG - chr1.3:43254503-43254522 MS.gene81771:intron 55.0%
GAATCGAACCTGAGACCTCG+AGG + chr1.3:43254479-43254498 None:intergenic 55.0%
TACCACCAGGCCAATCCAAG+TGG + chr1.3:43254428-43254447 None:intergenic 55.0%
!! ATCCACTTGGATTGGCCTGG+TGG - chr1.3:43254423-43254442 MS.gene81771:intron 55.0%
AGCGGCAAGAGCTGCTGCTA+TGG + chr1.3:43253783-43253802 None:intergenic 60.0%
GTCCCCATGCAACTGGACCA+AGG - chr1.3:43254129-43254148 MS.gene81771:intron 60.0%
TCGAACCTGAGACCTCGAGG+AGG + chr1.3:43254476-43254495 None:intergenic 60.0%
!! TTCCATCCTCCTGGCCTTGC+AGG + chr1.3:43253719-43253738 None:intergenic 60.0%
CACCTGCAAGGCCAGGAGGA+TGG - chr1.3:43253714-43253733 MS.gene81771:CDS 65.0%
GTGCTCCTCCTCGAGGTCTC+AGG - chr1.3:43254468-43254487 MS.gene81771:intron 65.0%
GTTGCAGCACCTGCAAGGCC+AGG - chr1.3:43253707-43253726 MS.gene81771:CDS 65.0%
TGTTCCCATCGAGGCGGCAG+CGG + chr1.3:43253801-43253820 None:intergenic 65.0%
! TGGGAGTGTGCTCCTCCTCG+AGG - chr1.3:43254461-43254480 MS.gene81771:intron 65.0%
CTTGCCGCTGCCGCCTCGAT+GGG - chr1.3:43253794-43253813 MS.gene81771:CDS 70.0%
GCAGCACCTGCAAGGCCAGG+AGG - chr1.3:43253710-43253729 MS.gene81771:CDS 70.0%
TCTTGCCGCTGCCGCCTCGA+TGG - chr1.3:43253793-43253812 MS.gene81771:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 43253647 43254840 43253647 ID=MS.gene81771
chr1.3 mRNA 43253647 43254840 43253647 ID=MS.gene81771.t1;Parent=MS.gene81771
chr1.3 exon 43254593 43254840 43254593 ID=MS.gene81771.t1.exon1;Parent=MS.gene81771.t1
chr1.3 CDS 43254593 43254840 43254593 ID=cds.MS.gene81771.t1;Parent=MS.gene81771.t1
chr1.3 exon 43254312 43254435 43254312 ID=MS.gene81771.t1.exon2;Parent=MS.gene81771.t1
chr1.3 CDS 43254312 43254435 43254312 ID=cds.MS.gene81771.t1;Parent=MS.gene81771.t1
chr1.3 exon 43253647 43253862 43253647 ID=MS.gene81771.t1.exon3;Parent=MS.gene81771.t1
chr1.3 CDS 43253647 43253862 43253647 ID=cds.MS.gene81771.t1;Parent=MS.gene81771.t1
Gene Sequence

>MS.gene81771

ATGTCAACCAAAATTGATATTCAAGAATCAAGCAAAGATACTAAAGGAAAAGCTGTTTTGGTTGCAGCACCTGCAAGGCCAGGAGGATGGAAAAAGGGCATAGCCATAATGGATTTCATTTTAAGGTTAGGTGCCATAGCAGCAGCTCTTGCCGCTGCCGCCTCGATGGGAACAAGTGATCAATCTCTTTCTTTCTTTACTCAGTTCTTTCAGTTTGAAGCTAGCTATGATAGCTTTCCTGCATTCCAGTATGTAACATTCAAAACTCTACTTTCTATGTCTTTCTAAGTCACATATAAGATCGGTTATGATCTGATGATAGTGTAAAACGTTTTACACTAATATGTATCAAAATTAGATTGTAATCTATATGCAATTTATTCACTTAATGCTTGAAAAATGCAGGTTTTTTCTTATTGCAATGGCTATAGTGGCTGGCTATTTGGTCTTGTCTCTACCTTTTTCTATAGTAGCCATCATACGTCCCCATGCAACTGGACCAAGGCTTTTTCTCATCATCCTAGACACCGTAAGCTCTCTATTTTCTAACTCTCGATCATTTCTTTGGCATAATAATGCGACATACCCCCTCCAGTCTCAAATATAAGTAAAAGTTAGGTCAACAAAAGTTGATGTATTTGATCCAAAAGTTGAATTAAATACATCTATTTTTGCTGACTCAACTTTTGTTTATATTTGAGACCGAAGCGAGTAGTAAACATCTAATTAGTGCTCACCTGCACGCTAGTATTAGCATAATTAGTTATGATTTTTCAATCCACTTGGATTGGCCTGGTGGTATTGACTTGTGATCTGGGAGTGTGCTCCTCCTCGAGGTCTCAGGTTCGATTCTCTCCAGTGCCAATTTAGGTGGGCTGGTTTAACTTCTTAAAAAAAAAAAATTAGGTGGGCTGGTTTAACTTCTTAAAAAAAAAAAAAATTAGTTATGATTTTTCTTTGTGGTGCTTATAATTGCAGGTATTTCTTACTCTGGCTACTGCTAGTGCTGCTTCAGCTGCTTCCATAGTTTACTTGGCACACAATGGAAATCAAGACACAAACTGGCTTGCCATTTGCAACCAATTTGGAGATTTTTGTGCACAGACAAGTGGAGCAGTTGTGGCATCATTTATTACTGTGGTTGTTTTAATCTTGTTGGTTGTGATGTCTGCCTTGGCTATTGGGAAGCATTGA

Protein sequence

>MS.gene81771.t1

MSTKIDIQESSKDTKGKAVLVAAPARPGGWKKGIAIMDFILRLGAIAAALAAAASMGTSDQSLSFFTQFFQFEASYDSFPAFQFFLIAMAIVAGYLVLSLPFSIVAIIRPHATGPRLFLIILDTVFLTLATASAASAASIVYLAHNGNQDTNWLAICNQFGDFCAQTSGAVVASFITVVVLILLVVMSALAIGKH