AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83689

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83689.t1 MTR_8g070930 86.612 366 2 1 1 366 1 319 0.0 640
MS.gene83689.t1 MTR_5g082700 43.810 210 105 5 161 366 384 584 6.64e-45 163
MS.gene83689.t1 MTR_5g082700 43.810 210 105 5 161 366 384 584 1.11e-44 163
MS.gene83689.t1 MTR_5g082700 43.810 210 105 5 161 366 384 584 1.42e-44 163
MS.gene83689.t1 MTR_3g086530 34.615 208 111 3 185 369 92 297 1.14e-37 138
MS.gene83689.t1 MTR_3g086530 34.615 208 111 3 185 369 92 297 2.44e-37 139
MS.gene83689.t1 MTR_3g076540 34.466 206 112 2 185 369 11 214 2.63e-37 137
MS.gene83689.t1 MTR_3g086530 34.615 208 111 3 185 369 92 297 4.39e-37 139
MS.gene83689.t1 MTR_3g076540 34.466 206 112 2 185 369 93 296 6.01e-37 138
MS.gene83689.t1 MTR_3g109390 35.122 205 111 2 185 369 73 275 1.18e-36 135
MS.gene83689.t1 MTR_3g109390 35.122 205 111 2 185 369 73 275 2.42e-36 136
MS.gene83689.t1 MTR_3g109390 35.122 205 111 2 185 369 51 253 2.47e-36 135
MS.gene83689.t1 MTR_3g109390 35.122 205 111 2 185 369 73 275 2.66e-36 136
MS.gene83689.t1 MTR_3g076540 34.171 199 108 2 192 369 1 197 4.04e-36 134
MS.gene83689.t1 MTR_3g076540 33.645 214 109 3 185 367 93 304 1.08e-35 135
MS.gene83689.t1 MTR_4g104620 36.275 204 105 4 185 366 97 297 1.81e-35 134
MS.gene83689.t1 MTR_4g104620 36.181 199 102 4 192 368 1 196 1.12e-34 130
MS.gene83689.t1 MTR_4g104620 36.181 199 102 4 192 368 10 205 1.36e-34 130
MS.gene83689.t1 MTR_3g109390 35.385 195 104 2 195 369 70 262 3.60e-34 130
MS.gene83689.t1 MTR_3g109390 35.385 195 104 2 195 369 92 284 4.44e-34 130
MS.gene83689.t1 MTR_1g111180 38.743 191 107 6 185 369 674 860 1.97e-31 126
MS.gene83689.t1 MTR_1g111180 38.743 191 107 6 185 369 675 861 2.03e-31 126
MS.gene83689.t1 MTR_6g074870 37.363 182 79 3 185 366 128 274 3.59e-29 115
MS.gene83689.t1 MTR_6g074905 37.363 182 79 3 185 366 897 1043 1.42e-28 117
MS.gene83689.t1 MTR_3g086530 32.967 182 97 3 211 369 1 180 1.10e-26 108
MS.gene83689.t1 MTR_1g061690 40.136 147 82 3 183 324 41 186 2.08e-23 102
MS.gene83689.t1 MTR_5g024270 39.310 145 82 3 185 324 37 180 2.51e-22 97.4
MS.gene83689.t1 MTR_5g024270 39.310 145 82 3 185 324 37 180 3.92e-22 97.4
MS.gene83689.t1 MTR_4g056390 37.589 141 82 3 186 321 39 178 2.31e-21 95.9
MS.gene83689.t1 MTR_6g074640 40.000 135 50 3 212 346 43 146 7.24e-21 90.9
MS.gene83689.t1 MTR_2g084555 37.762 143 83 3 186 323 40 181 1.52e-20 93.6
MS.gene83689.t1 MTR_0349s0030 46.429 112 54 2 259 366 54 163 2.63e-20 90.5
MS.gene83689.t1 MTR_4g061130 36.264 182 93 7 192 363 45 213 1.07e-18 86.7
MS.gene83689.t1 MTR_4g087620 35.359 181 94 7 192 362 61 228 1.53e-18 85.1
MS.gene83689.t1 MTR_8g069620 43.000 100 56 1 185 284 43 141 2.12e-18 83.2
MS.gene83689.t1 MTR_4g087620 35.165 182 95 7 192 363 61 229 2.99e-18 85.9
MS.gene83689.t1 MTR_8g012450 35.135 185 95 7 186 362 4 171 5.49e-18 85.1
MS.gene83689.t1 MTR_8g012450 35.135 185 95 7 186 362 4 171 5.74e-18 85.1
MS.gene83689.t1 MTR_5g026960 35.000 180 104 7 185 362 3 171 6.11e-18 85.1
MS.gene83689.t1 MTR_6g080470 31.720 186 107 7 185 363 13 185 8.91e-18 84.3
MS.gene83689.t1 MTR_8g073250 33.103 145 91 3 185 324 37 180 9.61e-18 84.7
MS.gene83689.t1 MTR_6g074750 48.718 78 38 1 289 366 17 92 5.33e-17 76.6
MS.gene83689.t1 MTR_4g094430 29.834 181 111 4 192 366 10 180 6.28e-17 80.9
MS.gene83689.t1 MTR_3g112210 32.642 193 108 7 180 362 26 206 6.28e-17 81.6
MS.gene83689.t1 MTR_3g054080 31.217 189 106 8 185 363 13 187 1.03e-16 81.3
MS.gene83689.t1 MTR_4g061320 31.892 185 97 6 192 363 46 214 1.06e-15 78.2
MS.gene83689.t1 MTR_5g010030 32.418 182 100 6 192 363 47 215 1.11e-15 78.2
MS.gene83689.t1 MTR_3g460950 33.523 176 107 4 192 363 38 207 2.57e-15 77.0
MS.gene83689.t1 MTR_3g060330 33.523 176 107 4 192 363 38 207 2.57e-15 77.0
MS.gene83689.t1 MTR_8g071230 32.418 182 106 7 185 362 3 171 4.74e-15 76.6
MS.gene83689.t1 MTR_2g032060 28.729 181 113 4 192 366 10 180 6.81e-15 74.7
MS.gene83689.t1 MTR_8g106960 30.319 188 114 7 182 360 114 293 1.95e-14 75.1
MS.gene83689.t1 MTR_8g106960 30.319 188 114 7 182 360 114 293 1.98e-14 75.1
MS.gene83689.t1 MTR_4g117490 30.688 189 106 7 185 360 24 200 2.41e-14 74.7
MS.gene83689.t1 MTR_3g086530 31.200 125 63 1 268 369 1 125 2.45e-14 72.8
MS.gene83689.t1 MTR_4g117490 30.811 185 111 6 185 360 24 200 3.03e-14 74.3
MS.gene83689.t1 MTR_8g432510 31.937 191 101 9 186 363 139 313 4.49e-14 73.6
MS.gene83689.t1 MTR_8g028720 29.744 195 113 7 180 363 30 211 1.29e-13 71.6
MS.gene83689.t1 MTR_8g098845 31.522 184 99 7 192 363 58 226 1.41e-13 71.6
MS.gene83689.t1 MTR_2g021330 30.159 189 107 7 185 360 24 200 1.42e-13 72.0
MS.gene83689.t1 MTR_2g021330 30.270 185 112 6 185 360 24 200 1.47e-13 72.4
MS.gene83689.t1 MTR_1g075610 32.086 187 101 8 192 363 24 199 9.96e-13 68.6
MS.gene83689.t1 MTR_8g086000 29.534 193 116 6 180 363 69 250 3.04e-12 67.8
MS.gene83689.t1 MTR_7g110060 30.208 192 103 9 186 363 75 249 3.59e-12 67.8
MS.gene83689.t1 MTR_8g086000 29.534 193 116 6 180 363 26 207 3.71e-12 67.4
MS.gene83689.t1 MTR_1g086370 30.208 192 103 9 186 363 75 249 5.39e-12 67.0
MS.gene83689.t1 MTR_1g086370 30.208 192 103 9 186 363 42 216 6.07e-12 66.6
MS.gene83689.t1 MTR_7g110060 30.208 192 103 9 186 363 75 249 7.30e-12 66.6
MS.gene83689.t1 MTR_1g084760 27.368 190 111 7 186 363 75 249 2.37e-11 65.1
MS.gene83689.t1 MTR_2g005290 32.919 161 95 6 182 339 6 156 2.42e-11 65.1
MS.gene83689.t1 MTR_4g007750 30.198 202 110 6 184 363 2 194 2.65e-11 64.3
MS.gene83689.t1 MTR_7g076030 28.571 189 110 6 186 363 34 208 2.76e-11 64.7
MS.gene83689.t1 MTR_1g048630 29.101 189 115 6 184 366 5 180 3.12e-11 65.5
MS.gene83689.t1 MTR_4g103810 29.775 178 111 5 192 363 114 283 4.74e-11 64.3
MS.gene83689.t1 MTR_2g083940 27.692 195 114 8 181 363 83 262 5.02e-11 64.3
MS.gene83689.t1 MTR_7g076030 28.571 189 110 6 186 363 141 315 5.08e-11 64.3
MS.gene83689.t1 MTR_4g103810 29.775 178 111 5 192 363 224 393 5.23e-11 64.3
MS.gene83689.t1 MTR_2g083940 27.128 188 114 6 186 363 34 208 6.26e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83689.t1 AT5G35980 76.718 262 57 2 105 366 9 266 4.50e-138 416
MS.gene83689.t1 AT5G35980 79.839 248 49 1 119 366 55 301 2.23e-137 410
MS.gene83689.t1 AT5G35980 79.839 248 49 1 119 366 55 301 3.22e-136 412
MS.gene83689.t1 AT2G40120 42.381 210 108 5 161 366 242 442 3.43e-43 158
MS.gene83689.t1 AT1G73450 44.624 186 96 3 185 366 840 1022 1.61e-40 153
MS.gene83689.t1 AT1G73460 44.624 186 96 3 185 366 857 1039 1.72e-40 152
MS.gene83689.t1 AT1G73460 44.624 186 96 3 185 366 857 1039 1.72e-40 152
MS.gene83689.t1 AT4G32660 36.634 202 106 2 185 366 70 269 9.83e-40 144
MS.gene83689.t1 AT4G32660 36.634 202 106 2 185 366 62 261 1.76e-39 144
MS.gene83689.t1 AT3G17750 44.086 186 97 3 185 366 826 1008 1.87e-39 150
MS.gene83689.t1 AT4G32660 36.634 202 106 2 185 366 70 269 1.95e-39 144
MS.gene83689.t1 AT4G24740 34.951 206 111 2 185 369 97 300 9.06e-38 138
MS.gene83689.t1 AT4G24740 34.951 206 111 2 185 369 97 300 1.88e-37 138
MS.gene83689.t1 AT4G24740 34.951 206 111 2 185 369 59 262 2.64e-37 139
MS.gene83689.t1 AT4G24740 35.176 199 106 2 192 369 1 197 3.50e-37 134
MS.gene83689.t1 AT4G24740 34.951 206 111 2 185 369 97 300 3.50e-37 139
MS.gene83689.t1 AT4G24740 35.176 199 106 2 192 369 1 197 1.03e-36 135
MS.gene83689.t1 AT4G24740 35.176 199 106 2 192 369 1 197 1.03e-36 135
MS.gene83689.t1 AT4G24740 35.176 199 106 2 192 369 1 197 1.03e-36 135
MS.gene83689.t1 AT3G53570 33.333 213 105 4 185 367 100 305 9.29e-34 130
MS.gene83689.t1 AT3G53570 33.333 213 105 4 185 367 100 305 9.29e-34 130
MS.gene83689.t1 AT3G53570 33.333 213 105 4 185 367 114 319 9.58e-34 130
MS.gene83689.t1 AT3G53570 33.333 213 105 4 185 367 114 319 9.58e-34 130
MS.gene83689.t1 AT3G53570 33.333 213 105 4 185 367 114 319 9.58e-34 130
MS.gene83689.t1 AT3G53640 35.616 219 124 7 158 369 300 508 1.03e-29 120
MS.gene83689.t1 AT3G25840 37.696 191 109 5 185 369 354 540 1.40e-29 120
MS.gene83689.t1 AT3G25840 37.696 191 109 5 185 369 616 802 2.09e-29 120
MS.gene83689.t1 AT1G13350 35.602 191 113 5 185 369 441 627 6.31e-29 119
MS.gene83689.t1 AT1G13350 35.602 191 113 5 185 369 468 654 8.09e-29 118
MS.gene83689.t1 AT1G13350 35.602 191 113 5 185 369 514 700 8.60e-29 118
MS.gene83689.t1 AT4G32660 34.591 159 82 2 228 366 1 157 2.86e-24 101
MS.gene83689.t1 AT2G17530 39.310 145 82 3 185 324 37 180 1.06e-22 98.2
MS.gene83689.t1 AT3G53030 37.162 148 87 3 182 324 38 184 2.92e-22 98.6
MS.gene83689.t1 AT5G22840 37.162 148 87 3 182 324 41 187 3.12e-22 98.6
MS.gene83689.t1 AT2G17530 39.310 145 82 3 185 324 37 180 3.29e-22 97.8
MS.gene83689.t1 AT2G17530 39.310 145 82 3 185 324 37 180 3.29e-22 97.8
MS.gene83689.t1 AT4G13020 37.968 187 91 7 184 362 2 171 8.09e-22 96.3
MS.gene83689.t1 AT4G13020 37.968 187 91 7 184 362 2 171 8.68e-22 95.9
MS.gene83689.t1 AT4G13020 37.968 187 91 7 184 362 2 171 8.93e-22 96.3
MS.gene83689.t1 AT4G13020 37.968 187 91 7 184 362 2 171 9.87e-22 96.3
MS.gene83689.t1 AT4G13020 38.172 186 90 7 185 362 11 179 1.37e-21 95.9
MS.gene83689.t1 AT4G13020 38.172 186 90 7 185 362 11 179 1.38e-21 95.9
MS.gene83689.t1 AT4G35500 37.931 145 84 3 185 324 37 180 9.75e-21 93.6
MS.gene83689.t1 AT3G44850 33.974 156 97 3 182 332 41 195 1.04e-20 94.0
MS.gene83689.t1 AT4G13020 37.306 193 93 8 184 362 2 180 6.18e-20 90.5
MS.gene83689.t1 AT4G13020 37.306 193 93 8 184 362 2 180 6.66e-20 91.3
MS.gene83689.t1 AT1G73690 31.720 186 107 6 185 363 10 182 4.01e-19 88.6
MS.gene83689.t1 AT1G18040 31.183 186 108 6 185 363 11 183 1.80e-18 86.3
MS.gene83689.t1 AT1G66750 33.333 186 104 7 185 363 12 184 1.06e-17 83.6
MS.gene83689.t1 AT2G43790 34.066 182 97 6 192 363 69 237 2.01e-17 83.6
MS.gene83689.t1 AT1G07880 32.308 195 108 7 180 363 26 207 4.06e-17 82.0
MS.gene83689.t1 AT3G45640 35.165 182 95 7 192 363 44 212 4.54e-17 82.0
MS.gene83689.t1 AT1G07880 32.308 195 108 7 180 363 26 207 5.09e-17 80.5
MS.gene83689.t1 AT5G45430 32.418 182 106 6 185 362 3 171 5.90e-17 82.4
MS.gene83689.t1 AT5G45430 32.418 182 106 6 185 362 3 171 6.38e-17 82.4
MS.gene83689.t1 AT4G11330 31.892 185 97 6 192 363 49 217 1.74e-16 80.5
MS.gene83689.t1 AT4G19110 33.152 184 102 6 185 362 3 171 2.32e-16 80.5
MS.gene83689.t1 AT4G19110 33.152 184 102 6 185 362 3 171 2.36e-16 80.5
MS.gene83689.t1 AT1G01560 30.769 195 111 7 180 363 33 214 6.57e-16 78.6
MS.gene83689.t1 AT1G01560 30.928 194 110 7 180 362 33 213 7.44e-16 77.4
MS.gene83689.t1 AT1G01560 30.928 194 110 7 180 362 33 213 7.44e-16 77.4
MS.gene83689.t1 AT1G01560 30.928 194 110 7 180 362 33 213 1.23e-15 77.8
MS.gene83689.t1 AT3G04530 29.474 190 127 4 179 366 4 188 2.42e-15 75.9
MS.gene83689.t1 AT4G35500 34.932 146 88 4 185 324 37 181 3.50e-15 77.0
MS.gene83689.t1 AT4G01370 31.122 196 107 9 180 362 36 216 4.41e-15 76.3
MS.gene83689.t1 AT1G10210 30.180 222 127 7 150 362 4 206 7.03e-15 75.5
MS.gene83689.t1 AT1G10210 30.180 222 127 7 150 362 4 206 7.03e-15 75.5
MS.gene83689.t1 AT1G10210 30.180 222 127 7 150 362 4 206 7.03e-15 75.5
MS.gene83689.t1 AT3G18040 30.319 188 114 5 182 360 14 193 1.15e-14 75.5
MS.gene83689.t1 AT3G18040 30.319 188 114 5 182 360 19 198 1.16e-14 75.5
MS.gene83689.t1 AT3G18040 30.319 188 114 5 182 360 128 307 1.26e-14 75.9
MS.gene83689.t1 AT3G18040 30.319 188 114 5 182 360 128 307 1.43e-14 75.5
MS.gene83689.t1 AT4G36450 31.183 186 117 4 180 362 26 203 1.88e-14 74.3
MS.gene83689.t1 AT1G18150 30.319 188 114 5 182 360 100 279 1.99e-14 75.1
MS.gene83689.t1 AT1G18150 30.319 188 114 5 182 360 100 279 1.99e-14 75.1
MS.gene83689.t1 AT1G18150 30.319 188 114 5 182 360 100 279 1.99e-14 75.1
MS.gene83689.t1 AT3G48750 28.108 185 116 5 192 369 10 184 5.59e-14 72.0
MS.gene83689.t1 AT5G19010 30.811 185 111 6 185 360 24 200 6.93e-14 73.2
MS.gene83689.t1 AT3G59790 31.868 182 101 6 192 363 66 234 7.64e-14 72.8
MS.gene83689.t1 AT1G59580 30.366 191 115 5 180 362 26 206 1.50e-13 71.6
MS.gene83689.t1 AT1G59580 30.366 191 115 5 180 362 26 206 1.50e-13 71.6
MS.gene83689.t1 AT1G08650 29.474 190 119 6 183 366 12 192 5.77e-13 68.9
MS.gene83689.t1 AT1G08650 29.474 190 119 6 183 366 12 192 5.89e-13 68.9
MS.gene83689.t1 AT2G18170 29.843 191 116 5 180 362 26 206 6.41e-13 69.7
MS.gene83689.t1 AT2G46070 31.633 196 106 9 180 362 68 248 1.22e-12 68.9
MS.gene83689.t1 AT4G00720 27.801 241 143 9 137 363 89 312 1.22e-12 69.3
MS.gene83689.t1 AT2G46070 31.633 196 106 9 180 362 34 214 1.33e-12 68.9
MS.gene83689.t1 AT2G46070 31.633 196 106 9 180 362 34 214 1.68e-12 68.2
MS.gene83689.t1 AT1G76540 31.217 189 100 9 192 363 20 195 2.77e-12 67.4
MS.gene83689.t1 AT1G20930 32.804 189 97 9 192 363 22 197 3.20e-12 67.0
MS.gene83689.t1 AT1G32320 29.412 170 104 5 191 359 53 207 1.39e-11 65.1
MS.gene83689.t1 AT1G50240 31.016 187 110 6 186 366 6 179 1.56e-11 66.6
MS.gene83689.t1 AT1G50240 31.016 187 110 6 186 366 6 179 1.56e-11 66.6
MS.gene83689.t1 AT1G53570 29.255 188 108 8 191 366 219 393 3.53e-11 65.1
MS.gene83689.t1 AT1G53570 29.255 188 108 8 191 366 219 393 3.53e-11 65.1
MS.gene83689.t1 AT1G53570 29.255 188 108 8 191 366 219 393 3.66e-11 65.1
MS.gene83689.t1 AT1G53570 29.255 188 108 8 191 366 219 393 3.72e-11 65.1
MS.gene83689.t1 AT1G53570 29.255 188 108 8 191 366 219 393 4.05e-11 64.7
MS.gene83689.t1 AT1G53570 29.255 188 108 8 191 366 219 393 4.06e-11 64.7
MS.gene83689.t1 AT3G15220 25.137 183 118 6 185 361 14 183 7.55e-11 63.9
MS.gene83689.t1 AT3G54180 30.348 201 109 8 184 363 2 192 9.13e-11 62.8

Find 66 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAACTTTGGATTGCATATT+TGG 0.086926 8.3:+31878905 None:intergenic
CACAAAAGCACGCAACTTTA+AGG 0.116671 8.3:+31880674 None:intergenic
TCTTATTCTGACAGTGAATT+TGG 0.182882 8.3:-31878794 MS.gene83689:CDS
GTGCATATGCTTTGAACTTC+TGG 0.223726 8.3:-31878235 MS.gene83689:CDS
CTCCTTGGCCATGGGACATT+TGG 0.283069 8.3:-31878573 MS.gene83689:CDS
TACTGTTTATTCATATATTC+AGG 0.292684 8.3:-31877588 MS.gene83689:intron
CTGATTCAGGCTGGCGTCCT+AGG 0.310235 8.3:-31880761 MS.gene83689:CDS
TCATTAAGCACTCCAGTAGA+TGG 0.327863 8.3:+31878841 None:intergenic
CTTAATTTCTGCTGACTTCA+CGG 0.359886 8.3:+31877646 None:intergenic
GGTATCTGAATTCCAGCATT+TGG 0.362547 8.3:+31878542 None:intergenic
AGATCTCCCGAGGTTCTTCT+TGG 0.369039 8.3:-31877449 MS.gene83689:CDS
ACGGCGTCTTCCTCTGATTC+AGG 0.371419 8.3:-31880774 MS.gene83689:CDS
GAAATTAAGATTATTGACTT+TGG 0.379182 8.3:-31877632 MS.gene83689:CDS
GGTCAGATTAAAGCACAAAT+TGG 0.390770 8.3:+31880008 None:intergenic
TCATCAGAATATTTAAACTT+TGG 0.391401 8.3:+31878892 None:intergenic
GATCTACTTTCTTTGTGTGT+TGG 0.394948 8.3:-31879252 MS.gene83689:CDS
TCCATTTAGAGAAAAGTAGA+AGG 0.403678 8.3:-31878765 MS.gene83689:intron
TGTTCAGTTGTTCTCTAAGC+AGG 0.422198 8.3:-31877966 MS.gene83689:intron
GGTTTGCTTTAAAGCTTTGT+TGG 0.424177 8.3:-31879215 MS.gene83689:intron
ACACTTGGGAAGTCATTTGC+AGG 0.425509 8.3:-31879274 MS.gene83689:CDS
GTATGTTTCAACTATTCCCT+TGG 0.427642 8.3:+31878927 None:intergenic
CTGACCGTTCCTTGCATTGG+AGG 0.447423 8.3:-31879991 MS.gene83689:intron
AGCTACTAATTTCATGGGTT+AGG 0.453179 8.3:+31877416 None:intergenic
TCATCGCCCCGCGGTACAAA+TGG 0.460000 8.3:+31880723 None:intergenic
TTGGACAAGTGGCCAAATGC+TGG 0.461235 8.3:-31878554 MS.gene83689:CDS
AATCTGACCGTTCCTTGCAT+TGG 0.467558 8.3:-31879994 MS.gene83689:CDS
TTGGGCTGGGTAGATGCTAA+TGG 0.474165 8.3:-31880031 MS.gene83689:intron
TCAACCTGCATACTATCAAC+AGG 0.475179 8.3:-31878487 MS.gene83689:CDS
CTACTACAACTACTACCAAC+AGG 0.489714 8.3:+31880837 None:intergenic
TGATTCAGGCTGGCGTCCTA+GGG 0.496536 8.3:-31880760 MS.gene83689:CDS
TAGAGATACTGTTGTCGAAA+CGG 0.502863 8.3:-31880793 MS.gene83689:CDS
GTCAAAGATCTCCTTGGCCA+TGG 0.510841 8.3:-31878582 MS.gene83689:CDS
GAACAACGAGTTAATGCTGC+TGG 0.512757 8.3:-31880702 MS.gene83689:CDS
TGACTTTGGATCAGCATGCA+TGG 0.530289 8.3:-31877618 MS.gene83689:CDS
GGAGCAAAAGCAAGCTCCCT+AGG 0.534255 8.3:+31880744 None:intergenic
CTGGCTCTATTAAAAGATGC+TGG 0.537424 8.3:-31877839 MS.gene83689:CDS
CGTCTTCCTCTGATTCAGGC+TGG 0.538738 8.3:-31880770 MS.gene83689:CDS
AAGCACTCCAGTAGATGGGC+TGG 0.538887 8.3:+31878846 None:intergenic
GTCCAAATGTCCCATGGCCA+AGG 0.543463 8.3:+31878571 None:intergenic
CCATGGGACATTTGGACAAG+TGG 0.548576 8.3:-31878565 MS.gene83689:CDS
TCTACTGGAGTGCTTAATGA+TGG 0.548930 8.3:-31878838 MS.gene83689:CDS
TGCATACTATCAACAGGCAC+TGG 0.549557 8.3:-31878481 MS.gene83689:CDS
TGATATCCAAGAAGAACCTC+GGG 0.551571 8.3:+31877443 None:intergenic
CATTAAGCACTCCAGTAGAT+GGG 0.555637 8.3:+31878842 None:intergenic
TCAAAGATCTCCTTGGCCAT+GGG 0.558703 8.3:-31878581 MS.gene83689:CDS
TATATTGTCAAAGATCTCCT+TGG 0.558829 8.3:-31878588 MS.gene83689:CDS
CTTCACAGCAACAAAACTGT+TGG 0.564297 8.3:+31878521 None:intergenic
CTGATATCCAAGAAGAACCT+CGG 0.573751 8.3:+31877442 None:intergenic
ACAATGTGATGCTTATCCTC+AGG 0.576747 8.3:+31878294 None:intergenic
AGGACGCCAGCCTGAATCAG+AGG 0.605108 8.3:+31880764 None:intergenic
GTAGATGAGATGCATACACT+TGG 0.608680 8.3:-31879289 MS.gene83689:intron
TGGCTTTGATAATGCAAACT+CGG 0.611062 8.3:-31878818 MS.gene83689:CDS
GATGAGGTGGTTAGACCTGT+TGG 0.613287 8.3:-31880852 MS.gene83689:CDS
CCACTTGTCCAAATGTCCCA+TGG 0.616191 8.3:+31878565 None:intergenic
GAATATATGAATAAACAGTA+CGG 0.624138 8.3:+31877591 None:intergenic
AGTGCCTGTTGATAGTATGC+AGG 0.633943 8.3:+31878483 None:intergenic
ATTCAGATACCTCCAATGCA+AGG 0.638760 8.3:+31879982 None:intergenic
GTGTTGGTGTGCTGATGACG+TGG 0.644595 8.3:-31879236 MS.gene83689:CDS
GTTGGTGGCAAGATTGACCA+AGG 0.648435 8.3:-31878944 MS.gene83689:CDS
AAATAAGAAGTACGATCCTG+AGG 0.655494 8.3:-31878310 MS.gene83689:CDS
GATACCTCCAATGCAAGGAA+CGG 0.663492 8.3:+31879987 None:intergenic
TAGATGAGATGCATACACTT+GGG 0.666375 8.3:-31879288 MS.gene83689:CDS
TCGATACTATAGATCTCCCG+AGG 0.707309 8.3:-31877459 MS.gene83689:CDS
TTGGTGGCAAGATTGACCAA+GGG 0.707753 8.3:-31878943 MS.gene83689:CDS
TTTGCTCCATTTGTACCGCG+GGG 0.712022 8.3:-31880729 MS.gene83689:CDS
ACTCGTTGTTCATCGCCCCG+CGG 0.714094 8.3:+31880714 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATAATTATTGTATTGA+AGG + chr8.3:31879563-31879582 None:intergenic 10.0%
!! TTATTAATTAACATAGATAA+AGG + chr8.3:31878013-31878032 None:intergenic 10.0%
!! AAAATAAAAATAACAACAAC+TGG + chr8.3:31879854-31879873 None:intergenic 15.0%
!! CAGGAAAAAAAAAAAAAAAA+AGG + chr8.3:31879947-31879966 None:intergenic 15.0%
!!! ATATAATTTGCATTATGATA+TGG - chr8.3:31879665-31879684 MS.gene83689:intron 15.0%
!!! ATGATTATTTTGTATATCAA+CGG - chr8.3:31880019-31880038 MS.gene83689:CDS 15.0%
!!! GGTTTTTACTTTAAAATTTT+AGG + chr8.3:31877992-31878011 None:intergenic 15.0%
!!! TATCAAAATGAATCATTTTA+GGG - chr8.3:31880277-31880296 MS.gene83689:intron 15.0%
!!! TTATCAAAATGAATCATTTT+AGG - chr8.3:31880276-31880295 MS.gene83689:intron 15.0%
!!! TTATGTTTTAAGATTTTATG+TGG - chr8.3:31880419-31880438 MS.gene83689:intron 15.0%
!! AAAAAGGAATATAAACTTAC+AGG + chr8.3:31877640-31877659 None:intergenic 20.0%
!! AAACACGTTAATTAACTATT+TGG - chr8.3:31878179-31878198 MS.gene83689:intron 20.0%
!! AAATAACCAAAATCACAAAA+AGG + chr8.3:31877656-31877675 None:intergenic 20.0%
!! ATTGACATTAAATAGTATAC+AGG - chr8.3:31880246-31880265 MS.gene83689:intron 20.0%
!! CAGATAATTATAATTACAAC+AGG + chr8.3:31880764-31880783 None:intergenic 20.0%
!! CTGTTGTAATTATAATTATC+TGG - chr8.3:31880762-31880781 MS.gene83689:CDS 20.0%
!! GAATATATGAATAAACAGTA+CGG + chr8.3:31880694-31880713 None:intergenic 20.0%
!! TACTGTTTATTCATATATTC+AGG - chr8.3:31880694-31880713 MS.gene83689:CDS 20.0%
!! TCATCAGAATATTTAAACTT+TGG + chr8.3:31879393-31879412 None:intergenic 20.0%
!! TTCAACATAACTAAAATTCT+TGG - chr8.3:31880162-31880181 MS.gene83689:intron 20.0%
!!! ATACTGATTATAGTTTTTGT+AGG - chr8.3:31879129-31879148 MS.gene83689:intron 20.0%
!!! ATCAAAATGAATCATTTTAG+GGG - chr8.3:31880278-31880297 MS.gene83689:intron 20.0%
!!! GAAATTAAGATTATTGACTT+TGG - chr8.3:31880650-31880669 MS.gene83689:intron 20.0%
!!! TATATTCCTTTTTGTGATTT+TGG - chr8.3:31877647-31877666 MS.gene83689:CDS 20.0%
!!! TGAAGTAACTATTTTAACAA+CGG - chr8.3:31879825-31879844 MS.gene83689:intron 20.0%
!!! TTGGAGGTAATTTATTTTTT+TGG - chr8.3:31877867-31877886 MS.gene83689:intron 20.0%
!!! TTTGAATTAGTTTGTGATTT+TGG - chr8.3:31877720-31877739 MS.gene83689:intron 20.0%
!!! TTTTTCAATTAATGTCTTGA+AGG - chr8.3:31878134-31878153 MS.gene83689:intron 20.0%
! AACTAAAGTATGTTCAAACT+GGG - chr8.3:31879091-31879110 MS.gene83689:intron 25.0%
! AGATAACAAAACCAACAAAA+AGG + chr8.3:31880376-31880395 None:intergenic 25.0%
! ATTCACTTTGGTATTCATAA+AGG + chr8.3:31879589-31879608 None:intergenic 25.0%
! ATTGAAGTTATTACTAGTTG+TGG - chr8.3:31878484-31878503 MS.gene83689:CDS 25.0%
! ATTGAGAATGTATCAAAACT+TGG - chr8.3:31880127-31880146 MS.gene83689:intron 25.0%
! TAAACTTTGGATTGCATATT+TGG + chr8.3:31879380-31879399 None:intergenic 25.0%
! TAACTAAAGTATGTTCAAAC+TGG - chr8.3:31879090-31879109 MS.gene83689:intron 25.0%
!! AAAACATTCTTTTATGTACG+AGG - chr8.3:31880492-31880511 MS.gene83689:intron 25.0%
!! AATACCAAAGTGAATTTTCT+GGG - chr8.3:31879594-31879613 MS.gene83689:intron 25.0%
!! GTACATAAAAGAATGTTTTC+TGG + chr8.3:31880491-31880510 None:intergenic 25.0%
!! TATAATATCTGAGGTATCAT+GGG + chr8.3:31878441-31878460 None:intergenic 25.0%
!! TATAGTTTTTGTAGGAAGTA+GGG - chr8.3:31879137-31879156 MS.gene83689:intron 25.0%
!! TTATAGTTTTTGTAGGAAGT+AGG - chr8.3:31879136-31879155 MS.gene83689:intron 25.0%
!! TTGTTAATTGTTACTTGCAT+TGG - chr8.3:31877689-31877708 MS.gene83689:intron 25.0%
!!! ATTGAAAAATGCATTGACTA+AGG + chr8.3:31878123-31878142 None:intergenic 25.0%
!!! GGTAATTTATTTTTTTGGTG+CGG - chr8.3:31877872-31877891 MS.gene83689:intron 25.0%
!!! GTGTTTTTTTGTTTTTGTGT+GGG - chr8.3:31877828-31877847 MS.gene83689:CDS 25.0%
!!! TGAATATAGTGTTTTATTGG+AGG - chr8.3:31877851-31877870 MS.gene83689:CDS 25.0%
!!! TGTGTTTTTTTGTTTTTGTG+TGG - chr8.3:31877827-31877846 MS.gene83689:CDS 25.0%
!!! TTAGTTTGTGATTTTGGAAA+TGG - chr8.3:31877726-31877745 MS.gene83689:intron 25.0%
!!! TTGAAAAATGCATTGACTAA+GGG + chr8.3:31878122-31878141 None:intergenic 25.0%
!!! TTTTAAGATTTTATGTGGAC+TGG - chr8.3:31880424-31880443 MS.gene83689:intron 25.0%
AAAGCCAGACAAATATCAAA+CGG + chr8.3:31880103-31880122 None:intergenic 30.0%
AAGTGACATTTACTTAAGGT+GGG - chr8.3:31878346-31878365 MS.gene83689:intron 30.0%
ACAAAAAGGACTAAACAGAA+AGG + chr8.3:31880362-31880381 None:intergenic 30.0%
ACTAAAGTATGTTCAAACTG+GGG - chr8.3:31879092-31879111 MS.gene83689:intron 30.0%
ATCCTATATCAAAATTCTGC+TGG + chr8.3:31879232-31879251 None:intergenic 30.0%
CAATACCAAAATATGCAAGA+GGG + chr8.3:31880230-31880249 None:intergenic 30.0%
CTCTAGTATTTGATCGAATT+GGG - chr8.3:31878942-31878961 MS.gene83689:CDS 30.0%
GATACCTCAGATATTATACT+AGG - chr8.3:31878443-31878462 MS.gene83689:intron 30.0%
GGTTAAGTGACATTTACTTA+AGG - chr8.3:31878342-31878361 MS.gene83689:intron 30.0%
GTAATATGACAAAAAGTCGT+GGG - chr8.3:31878369-31878388 MS.gene83689:intron 30.0%
GTTTATTGAGAGAAACAATG+AGG - chr8.3:31878528-31878547 MS.gene83689:CDS 30.0%
TAAGTGACATTTACTTAAGG+TGG - chr8.3:31878345-31878364 MS.gene83689:intron 30.0%
TAATATGACAAAAAGTCGTG+GGG - chr8.3:31878370-31878389 MS.gene83689:intron 30.0%
TATATTGTCAAAGATCTCCT+TGG - chr8.3:31879694-31879713 MS.gene83689:intron 30.0%
TCAATACCAAAATATGCAAG+AGG + chr8.3:31880231-31880250 None:intergenic 30.0%
TCCATTTAGAGAAAAGTAGA+AGG - chr8.3:31879517-31879536 MS.gene83689:intron 30.0%
TCTAGTATTTGATCGAATTG+GGG - chr8.3:31878943-31878962 MS.gene83689:CDS 30.0%
TCTCTAGTATTTGATCGAAT+TGG - chr8.3:31878941-31878960 MS.gene83689:CDS 30.0%
TCTTATTCTGACAGTGAATT+TGG - chr8.3:31879488-31879507 MS.gene83689:intron 30.0%
TGAGTCAAAACTCTTATCTA+TGG - chr8.3:31878042-31878061 MS.gene83689:intron 30.0%
TTCAATTTCCTATGCTTGAA+TGG + chr8.3:31878470-31878489 None:intergenic 30.0%
TTCTTTGATCTCACTATTAC+TGG + chr8.3:31878738-31878757 None:intergenic 30.0%
! AGTAAATGTCACTTAACCAA+CGG + chr8.3:31878340-31878359 None:intergenic 30.0%
! AGTATTGAATGTTGTGGAAA+TGG - chr8.3:31878649-31878668 MS.gene83689:intron 30.0%
! ATACCAAAGTGAATTTTCTG+GGG - chr8.3:31879595-31879614 MS.gene83689:intron 30.0%
! GAATACCAAAGTGAATTTTC+TGG - chr8.3:31879593-31879612 MS.gene83689:intron 30.0%
! GGGTGAATATAGTGTTTTAT+TGG - chr8.3:31877848-31877867 MS.gene83689:CDS 30.0%
! GTATAATATCTGAGGTATCA+TGG + chr8.3:31878442-31878461 None:intergenic 30.0%
! TTCTGTTTAGTCCTTTTTGT+TGG - chr8.3:31880362-31880381 MS.gene83689:intron 30.0%
!! AACCAGCAGAATTTTGATAT+AGG - chr8.3:31879227-31879246 MS.gene83689:CDS 30.0%
!! ACCTTCTACTTTTCTCTAAA+TGG + chr8.3:31879521-31879540 None:intergenic 30.0%
!! GATCTAAGTATTGAATGTTG+TGG - chr8.3:31878643-31878662 MS.gene83689:intron 30.0%
!! TAAGATGCTAGTCATTTTCT+TGG - chr8.3:31879873-31879892 MS.gene83689:intron 30.0%
!!! TTGACAGTTTTTGACTTGAA+AGG - chr8.3:31877777-31877796 MS.gene83689:intron 30.0%
AATTGTGAATTTGCAGTTGG+TGG - chr8.3:31879323-31879342 MS.gene83689:intron 35.0%
ACGTTGTTGTCTTATCTAGT+TGG - chr8.3:31878232-31878251 MS.gene83689:CDS 35.0%
AGATCCATAAAACGCTTTGT+CGG - chr8.3:31878092-31878111 MS.gene83689:intron 35.0%
AGGTATGTCAATTCTTTGTG+TGG - chr8.3:31880336-31880355 MS.gene83689:intron 35.0%
AGTGACATTTACTTAAGGTG+GGG - chr8.3:31878347-31878366 MS.gene83689:intron 35.0%
ATATCAAACGGCAAAGAAGT+TGG + chr8.3:31880091-31880110 None:intergenic 35.0%
ATGAAACTACCAAGTCATCA+AGG + chr8.3:31878694-31878713 None:intergenic 35.0%
CCAATATTGTCAATGTTGGA+TGG - chr8.3:31877966-31877985 MS.gene83689:intron 35.0%
CCATCCAACATTGACAATAT+TGG + chr8.3:31877969-31877988 None:intergenic 35.0%
CGAACCAATATTGTCAATGT+TGG - chr8.3:31877962-31877981 MS.gene83689:intron 35.0%
CGTTGTTGTCTTATCTAGTT+GGG - chr8.3:31878233-31878252 MS.gene83689:CDS 35.0%
CTAGTATTTGATCGAATTGG+GGG - chr8.3:31878944-31878963 MS.gene83689:CDS 35.0%
CTCAATTGTGAATTTGCAGT+TGG - chr8.3:31879320-31879339 MS.gene83689:intron 35.0%
GATCTACTTTCTTTGTGTGT+TGG - chr8.3:31879030-31879049 MS.gene83689:intron 35.0%
GGTAATATGACAAAAAGTCG+TGG - chr8.3:31878368-31878387 MS.gene83689:intron 35.0%
GGTATCTGAATCATCATGTA+AGG - chr8.3:31878312-31878331 MS.gene83689:CDS 35.0%
GGTCAGATTAAAGCACAAAT+TGG + chr8.3:31878277-31878296 None:intergenic 35.0%
GTATGTTTCAACTATTCCCT+TGG + chr8.3:31879358-31879377 None:intergenic 35.0%
TAGAGATACTGTTGTCGAAA+CGG - chr8.3:31877489-31877508 MS.gene83689:intron 35.0%
TAGATGAGATGCATACACTT+GGG - chr8.3:31878994-31879013 MS.gene83689:intron 35.0%
TATCCCCAGAAAATTCACTT+TGG + chr8.3:31879601-31879620 None:intergenic 35.0%
TGGTCCTAGTATAATATCTG+AGG + chr8.3:31878450-31878469 None:intergenic 35.0%
TTCTCATTGATGTGACTGTT+TGG - chr8.3:31879266-31879285 MS.gene83689:CDS 35.0%
! AAATAAGAAGTACGATCCTG+AGG - chr8.3:31879972-31879991 MS.gene83689:intron 35.0%
! CATTCTTTTATGTACGAGGT+TGG - chr8.3:31880496-31880515 MS.gene83689:intron 35.0%
! CGTACTTCTTATTTAACTGC+AGG + chr8.3:31879966-31879985 None:intergenic 35.0%
! GAGCATCAGTTGAAACTTTT+AGG + chr8.3:31879303-31879322 None:intergenic 35.0%
! TCAAAATTCTGCTGGTTACA+TGG + chr8.3:31879224-31879243 None:intergenic 35.0%
! TCAACCTTAACTTTGGGAAA+CGG - chr8.3:31878773-31878792 MS.gene83689:CDS 35.0%
! TTTGCCGTTTGATATTTGTC+TGG - chr8.3:31880096-31880115 MS.gene83689:intron 35.0%
!! CTTAATTTCTGCTGACTTCA+CGG + chr8.3:31880639-31880658 None:intergenic 35.0%
!! GAGATCAAAGAAGCTTTGTT+AGG - chr8.3:31878746-31878765 MS.gene83689:intron 35.0%
!! GAGGTTGGTTTCTAACATAA+AGG - chr8.3:31880511-31880530 MS.gene83689:intron 35.0%
!! GGTTTGCTTTAAAGCTTTGT+TGG - chr8.3:31879067-31879086 MS.gene83689:intron 35.0%
!! TGGCTTTGATAATGCAAACT+CGG - chr8.3:31879464-31879483 MS.gene83689:intron 35.0%
!!! ATTTTTTTGGTGCGGTTGTT+GGG - chr8.3:31877880-31877899 MS.gene83689:intron 35.0%
!!! TATTTTTTTGGTGCGGTTGT+TGG - chr8.3:31877879-31877898 MS.gene83689:intron 35.0%
!!! TGGGTGGAACAATTTTTTAC+CGG + chr8.3:31877919-31877938 None:intergenic 35.0%
AATGTGTCACACTAACCATC+TGG - chr8.3:31879624-31879643 MS.gene83689:intron 40.0%
ACAATGTGATGCTTATCCTC+AGG + chr8.3:31879991-31880010 None:intergenic 40.0%
ACGGAACTGTGCACAAAATA+AGG + chr8.3:31880620-31880639 None:intergenic 40.0%
AGGAGAGTCAACCTTAACTT+TGG - chr8.3:31878766-31878785 MS.gene83689:CDS 40.0%
AGTGAAGTCGCATGCAAATA+AGG + chr8.3:31878594-31878613 None:intergenic 40.0%
ATTACAATCTCTGTCGAGAG+CGG - chr8.3:31880723-31880742 MS.gene83689:CDS 40.0%
ATTCAGATACCTCCAATGCA+AGG + chr8.3:31878303-31878322 None:intergenic 40.0%
ATTTGCATGCGACTTCACTA+TGG - chr8.3:31878595-31878614 MS.gene83689:intron 40.0%
CAAAGAAGTTGGCAACTTAC+AGG + chr8.3:31880080-31880099 None:intergenic 40.0%
CATTAAGCACTCCAGTAGAT+GGG + chr8.3:31879443-31879462 None:intergenic 40.0%
CCTCACATGATACCAAAAGA+AGG - chr8.3:31878859-31878878 MS.gene83689:CDS 40.0%
CTACTACAACTACTACCAAC+AGG + chr8.3:31877448-31877467 None:intergenic 40.0%
CTGATATCCAAGAAGAACCT+CGG + chr8.3:31880843-31880862 None:intergenic 40.0%
CTTCACAGCAACAAAACTGT+TGG + chr8.3:31879764-31879783 None:intergenic 40.0%
GACAACAACGTCAGATAAAC+CGG + chr8.3:31878223-31878242 None:intergenic 40.0%
GATGGTTAGTGTGACACATT+TGG + chr8.3:31879624-31879643 None:intergenic 40.0%
GCTAGAAAGCAAGTGTAAGA+AGG - chr8.3:31878506-31878525 MS.gene83689:CDS 40.0%
GGAGAGTCAACCTTAACTTT+GGG - chr8.3:31878767-31878786 MS.gene83689:CDS 40.0%
GGTAAAAAATTGTTCCACCC+AGG - chr8.3:31877918-31877937 MS.gene83689:intron 40.0%
GGTATCTGAATTCCAGCATT+TGG + chr8.3:31879743-31879762 None:intergenic 40.0%
GTAGATGAGATGCATACACT+TGG - chr8.3:31878993-31879012 MS.gene83689:intron 40.0%
GTTTCTCTTTCGTGATGTTC+CGG - chr8.3:31878201-31878220 MS.gene83689:intron 40.0%
TACCAAAATATGCAAGAGGG+AGG + chr8.3:31880227-31880246 None:intergenic 40.0%
TACTAGGACCATTCAAGCAT+AGG - chr8.3:31878459-31878478 MS.gene83689:CDS 40.0%
TCAACCTGCATACTATCAAC+AGG - chr8.3:31879795-31879814 MS.gene83689:intron 40.0%
TCATTAAGCACTCCAGTAGA+TGG + chr8.3:31879444-31879463 None:intergenic 40.0%
TCCAACTCAGTTATCAAGGT+TGG - chr8.3:31878563-31878582 MS.gene83689:CDS 40.0%
TGATATCCAAGAAGAACCTC+GGG + chr8.3:31880842-31880861 None:intergenic 40.0%
TGTTCAGTTGTTCTCTAAGC+AGG - chr8.3:31880316-31880335 MS.gene83689:intron 40.0%
TTCTTGGACGAACTATGCAA+TGG - chr8.3:31880178-31880197 MS.gene83689:intron 40.0%
TTGTCTTATCTAGTTGGGCT+GGG - chr8.3:31878238-31878257 MS.gene83689:CDS 40.0%
TTTGCATGCGACTTCACTAT+GGG - chr8.3:31878596-31878615 MS.gene83689:intron 40.0%
! ACCAACCTTGATAACTGAGT+TGG + chr8.3:31878567-31878586 None:intergenic 40.0%
! ATCGACTCTGCACAAAAAAC+AGG + chr8.3:31880808-31880827 None:intergenic 40.0%
! CACAAAAGCACGCAACTTTA+AGG + chr8.3:31877611-31877630 None:intergenic 40.0%
! CCTTCTTTTGGTATCATGTG+AGG + chr8.3:31878862-31878881 None:intergenic 40.0%
! GTGCATATGCTTTGAACTTC+TGG - chr8.3:31880047-31880066 MS.gene83689:intron 40.0%
! TCTACTGGAGTGCTTAATGA+TGG - chr8.3:31879444-31879463 MS.gene83689:intron 40.0%
! TGACTTTTAGGCAAACCACA+CGG - chr8.3:31878892-31878911 MS.gene83689:CDS 40.0%
!! ATCATCATGTAAGGTACCGT+TGG - chr8.3:31878321-31878340 MS.gene83689:intron 40.0%
!! CATACCGACAAAGCGTTTTA+TGG + chr8.3:31878099-31878118 None:intergenic 40.0%
!! CATTTTAGGGGATTGTCACT+TGG - chr8.3:31880290-31880309 MS.gene83689:intron 40.0%
!! CTGGCTCTATTAAAAGATGC+TGG - chr8.3:31880443-31880462 MS.gene83689:intron 40.0%
!! GTATCATGGGTTATTGACTC+AGG + chr8.3:31878428-31878447 None:intergenic 40.0%
!! TCTTGAAGGAGCATTAGATG+TGG - chr8.3:31878148-31878167 MS.gene83689:intron 40.0%
!!! GGGCTGGTCAAAAATCTTTT+GGG + chr8.3:31879423-31879442 None:intergenic 40.0%
!!! TGGGCTGGTCAAAAATCTTT+TGG + chr8.3:31879424-31879443 None:intergenic 40.0%
AATCTGACCGTTCCTTGCAT+TGG - chr8.3:31878288-31878307 MS.gene83689:CDS 45.0%
AATGCCATTGCGGTGAACAT+AGG - chr8.3:31878827-31878846 MS.gene83689:CDS 45.0%
ACACCCATGAGAAGCTTCTA+AGG - chr8.3:31879902-31879921 MS.gene83689:intron 45.0%
ACACTTGGGAAGTCATTTGC+AGG - chr8.3:31879008-31879027 MS.gene83689:intron 45.0%
AGTGCCTTAGAAGCTTCTCA+TGG + chr8.3:31879909-31879928 None:intergenic 45.0%
CACTATGGGATCAGAGTTTG+AGG - chr8.3:31878610-31878629 MS.gene83689:intron 45.0%
CCATATCAATGCACCAACCA+AGG + chr8.3:31878974-31878993 None:intergenic 45.0%
GAACAACGAGTTAATGCTGC+TGG - chr8.3:31877580-31877599 MS.gene83689:intron 45.0%
GAGAAACAATGAGGTCCTGT+AGG - chr8.3:31878537-31878556 MS.gene83689:CDS 45.0%
GATACCTCCAATGCAAGGAA+CGG + chr8.3:31878298-31878317 None:intergenic 45.0%
GGGAGGCAAACAACAAATCT+AGG + chr8.3:31880210-31880229 None:intergenic 45.0%
GTGCCTTAGAAGCTTCTCAT+GGG + chr8.3:31879908-31879927 None:intergenic 45.0%
GTGCTCCAACTCAGTTATCA+AGG - chr8.3:31878559-31878578 MS.gene83689:CDS 45.0%
GTTGGGTGCTAAGTAAACTC+CGG - chr8.3:31877897-31877916 MS.gene83689:intron 45.0%
GTTGTCTTATCTAGTTGGGC+TGG - chr8.3:31878237-31878256 MS.gene83689:CDS 45.0%
TCAAAGATCTCCTTGGCCAT+GGG - chr8.3:31879701-31879720 MS.gene83689:intron 45.0%
TCATCAAGGAAAACCAGCTC+AGG + chr8.3:31878680-31878699 None:intergenic 45.0%
TCGATACTATAGATCTCCCG+AGG - chr8.3:31880823-31880842 MS.gene83689:CDS 45.0%
TGACTTTGGATCAGCATGCA+TGG - chr8.3:31880664-31880683 MS.gene83689:intron 45.0%
TGCATACTATCAACAGGCAC+TGG - chr8.3:31879801-31879820 MS.gene83689:intron 45.0%
TTACAATCTCTGTCGAGAGC+GGG - chr8.3:31880724-31880743 MS.gene83689:CDS 45.0%
! CCTTGGTTGGTGCATTGATA+TGG - chr8.3:31878971-31878990 MS.gene83689:intron 45.0%
! CTTTTGCTCCATTTGTACCG+CGG - chr8.3:31877551-31877570 MS.gene83689:intron 45.0%
! GACGTAGTGATTTTGCCAGA+TGG + chr8.3:31879642-31879661 None:intergenic 45.0%
! TGCCTCCCTCTTGCATATTT+TGG - chr8.3:31880222-31880241 MS.gene83689:intron 45.0%
! TTGGTGGCAAGATTGACCAA+GGG - chr8.3:31879339-31879358 MS.gene83689:intron 45.0%
! TTTTGCTCCATTTGTACCGC+GGG - chr8.3:31877552-31877571 MS.gene83689:intron 45.0%
! TTTTTGACCAGCCCATCTAC+TGG - chr8.3:31879429-31879448 MS.gene83689:intron 45.0%
!! AGTGCCTGTTGATAGTATGC+AGG + chr8.3:31879802-31879821 None:intergenic 45.0%
!!! GCTGGTTTTCCTTGATGACT+TGG - chr8.3:31878682-31878701 MS.gene83689:intron 45.0%
AAACCACACGGCGCATATGA+TGG - chr8.3:31878904-31878923 MS.gene83689:CDS 50.0%
AACTCTCTCCGACTAACTCC+TGG + chr8.3:31877939-31877958 None:intergenic 50.0%
AATTGGGGGATCACCTTGGT+TGG - chr8.3:31878958-31878977 MS.gene83689:intron 50.0%
ACGATGCAGATGAGCAAGTC+GGG - chr8.3:31878804-31878823 MS.gene83689:CDS 50.0%
ACTCTCTCCGACTAACTCCT+GGG + chr8.3:31877938-31877957 None:intergenic 50.0%
AGATCTCCCGAGGTTCTTCT+TGG - chr8.3:31880833-31880852 MS.gene83689:CDS 50.0%
ATCGAATTGGGGGATCACCT+TGG - chr8.3:31878954-31878973 MS.gene83689:intron 50.0%
CCACTTGTCCAAATGTCCCA+TGG + chr8.3:31879720-31879739 None:intergenic 50.0%
CCATGGGACATTTGGACAAG+TGG - chr8.3:31879717-31879736 MS.gene83689:intron 50.0%
CCATTGCGGTGAACATAGGA+AGG - chr8.3:31878831-31878850 MS.gene83689:CDS 50.0%
CCTTCCTATGTTCACCGCAA+TGG + chr8.3:31878834-31878853 None:intergenic 50.0%
GACGATGCAGATGAGCAAGT+CGG - chr8.3:31878803-31878822 MS.gene83689:CDS 50.0%
GATGAGGTGGTTAGACCTGT+TGG - chr8.3:31877430-31877449 MS.gene83689:CDS 50.0%
GCTCATCTGCATCGTCTATG+CGG + chr8.3:31878799-31878818 None:intergenic 50.0%
GGAAATGGTTGAACCTGAGC+TGG - chr8.3:31878664-31878683 MS.gene83689:intron 50.0%
GTCAAAGATCTCCTTGGCCA+TGG - chr8.3:31879700-31879719 MS.gene83689:intron 50.0%
TGTTCCACCCAGGAGTTAGT+CGG - chr8.3:31877928-31877947 MS.gene83689:intron 50.0%
TTGGACAAGTGGCCAAATGC+TGG - chr8.3:31879728-31879747 MS.gene83689:intron 50.0%
TTTGCTCCATTTGTACCGCG+GGG - chr8.3:31877553-31877572 MS.gene83689:intron 50.0%
! AACTGAGTTGGAGCACCTAC+AGG + chr8.3:31878555-31878574 None:intergenic 50.0%
! CAGACTTGCGTGCCTTCTTT+TGG + chr8.3:31878874-31878893 None:intergenic 50.0%
! GGCACGCAAGTCTGACTTTT+AGG - chr8.3:31878880-31878899 MS.gene83689:CDS 50.0%
! TTGGGCTGGGTAGATGCTAA+TGG - chr8.3:31878251-31878270 MS.gene83689:CDS 50.0%
!! GTTGGTGGCAAGATTGACCA+AGG - chr8.3:31879338-31879357 MS.gene83689:intron 50.0%
AAGCACTCCAGTAGATGGGC+TGG + chr8.3:31879439-31879458 None:intergenic 55.0%
ACGGCGTCTTCCTCTGATTC+AGG - chr8.3:31877508-31877527 MS.gene83689:intron 55.0%
CGTCTTCCTCTGATTCAGGC+TGG - chr8.3:31877512-31877531 MS.gene83689:intron 55.0%
CTCCTTGGCCATGGGACATT+TGG - chr8.3:31879709-31879728 MS.gene83689:intron 55.0%
CTGACCGTTCCTTGCATTGG+AGG - chr8.3:31878291-31878310 MS.gene83689:CDS 55.0%
GCAAGTCGGGAATGCCATTG+CGG - chr8.3:31878817-31878836 MS.gene83689:CDS 55.0%
GCGCATATGATGGCAGCTGA+TGG - chr8.3:31878914-31878933 MS.gene83689:CDS 55.0%
GCGGCCGTTTCCCAAAGTTA+AGG + chr8.3:31878780-31878799 None:intergenic 55.0%
GGAGCAAAAGCAAGCTCCCT+AGG + chr8.3:31877541-31877560 None:intergenic 55.0%
GTCCAAATGTCCCATGGCCA+AGG + chr8.3:31879714-31879733 None:intergenic 55.0%
TGATTCAGGCTGGCGTCCTA+GGG - chr8.3:31877522-31877541 MS.gene83689:intron 55.0%
! GTGTTGGTGTGCTGATGACG+TGG - chr8.3:31879046-31879065 MS.gene83689:intron 55.0%
ACTCGTTGTTCATCGCCCCG+CGG + chr8.3:31877571-31877590 None:intergenic 60.0%
AGGACGCCAGCCTGAATCAG+AGG + chr8.3:31877521-31877540 None:intergenic 60.0%
CTCTCCGACTAACTCCTGGG+TGG + chr8.3:31877935-31877954 None:intergenic 60.0%
CTGATTCAGGCTGGCGTCCT+AGG - chr8.3:31877521-31877540 MS.gene83689:intron 60.0%
CTGCCATCATATGCGCCGTG+TGG + chr8.3:31878910-31878929 None:intergenic 60.0%
TCATCGCCCCGCGGTACAAA+TGG + chr8.3:31877562-31877581 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 31877427 31880877 31877427 ID=MS.gene83689
chr8.3 mRNA 31877427 31880877 31877427 ID=MS.gene83689.t1;Parent=MS.gene83689
chr8.3 exon 31880665 31880877 31880665 ID=MS.gene83689.t1.exon1;Parent=MS.gene83689.t1
chr8.3 CDS 31880665 31880877 31880665 ID=cds.MS.gene83689.t1;Parent=MS.gene83689.t1
chr8.3 exon 31879992 31880040 31879992 ID=MS.gene83689.t1.exon2;Parent=MS.gene83689.t1
chr8.3 CDS 31879992 31880040 31879992 ID=cds.MS.gene83689.t1;Parent=MS.gene83689.t1
chr8.3 exon 31879216 31879307 31879216 ID=MS.gene83689.t1.exon3;Parent=MS.gene83689.t1
chr8.3 CDS 31879216 31879307 31879216 ID=cds.MS.gene83689.t1;Parent=MS.gene83689.t1
chr8.3 exon 31878766 31878965 31878766 ID=MS.gene83689.t1.exon4;Parent=MS.gene83689.t1
chr8.3 CDS 31878766 31878965 31878766 ID=cds.MS.gene83689.t1;Parent=MS.gene83689.t1
chr8.3 exon 31878458 31878611 31878458 ID=MS.gene83689.t1.exon5;Parent=MS.gene83689.t1
chr8.3 CDS 31878458 31878611 31878458 ID=cds.MS.gene83689.t1;Parent=MS.gene83689.t1
chr8.3 exon 31878225 31878334 31878225 ID=MS.gene83689.t1.exon6;Parent=MS.gene83689.t1
chr8.3 CDS 31878225 31878334 31878225 ID=cds.MS.gene83689.t1;Parent=MS.gene83689.t1
chr8.3 exon 31877967 31878036 31877967 ID=MS.gene83689.t1.exon7;Parent=MS.gene83689.t1
chr8.3 CDS 31877967 31878036 31877967 ID=cds.MS.gene83689.t1;Parent=MS.gene83689.t1
chr8.3 exon 31877791 31877873 31877791 ID=MS.gene83689.t1.exon8;Parent=MS.gene83689.t1
chr8.3 CDS 31877791 31877873 31877791 ID=cds.MS.gene83689.t1;Parent=MS.gene83689.t1
chr8.3 exon 31877589 31877670 31877589 ID=MS.gene83689.t1.exon9;Parent=MS.gene83689.t1
chr8.3 CDS 31877589 31877670 31877589 ID=cds.MS.gene83689.t1;Parent=MS.gene83689.t1
chr8.3 exon 31877427 31877483 31877427 ID=MS.gene83689.t1.exon10;Parent=MS.gene83689.t1
chr8.3 CDS 31877427 31877483 31877427 ID=cds.MS.gene83689.t1;Parent=MS.gene83689.t1
Gene Sequence

>MS.gene83689

ATGGATGAGGTGGTTAGACCTGTTGGTAGTAGTTGTAGTAGTGAAGTAGAGCATACTCAGTCTAGAGATACTGTTGTCGAAACGGCGTCTTCCTCTGATTCAGGCTGGCGTCCTAGGGAGCTTGCTTTTGCTCCATTTGTACCGCGGGGCGATGAACAACGAGTTAATGCTGCTGGAAAGTCCTTAAAGTTGCGTGCTTTTGTGAGAAAACCTGTAAGTTTATATTCCTTTTTGTGATTTTGGTTATTTATATTTGCTATTGTTGTTAATTGTTACTTGCATTGGTGTGTGATTTTGAATTAGTTTGTGATTTTGGAAATGGTTGTGTGAATTGTGACAAGATTGTTGCTTTGACAGTTTTTGACTTGAAAGGTGTTTTTTTTTTTTTCAGTGAGAAAGTTGTGTTTTTTTGTTTTTGTGTGGGTGAATATAGTGTTTTATTGGAGGTAATTTATTTTTTTGGTGCGGTTGTTGGGTGCTAAGTAAACTCCGGTAAAAAATTGTTCCACCCAGGAGTTAGTCGGAGAGAGTTCGTCGAACCAATATTGTCAATGTTGGATGGCCTAAAATTTTAAAGTAAAAACCTTTATCTATGTTAATTAATAATCTGAATAATGAGTCAAAACTCTTATCTATGGATATCTATCATTCATAAGTTGATAGTAAGATCCATAAAACGCTTTGTCGGTATGCCCTTAGTCAATGCATTTTTCAATTAATGTCTTGAAGGAGCATTAGATGTGGTTATCAATAAACACGTTAATTAACTATTTGGTTTCTCTTTCGTGATGTTCCGGTTTATCTGACGTTGTTGTCTTATCTAGTTGGGCTGGGTAGATGCTAATGGCCAATTTGTGCTTTAATCTGACCGTTCCTTGCATTGGAGGTATCTGAATCATCATGTAAGGTACCGTTGGTTAAGTGACATTTACTTAAGGTGGGGTAATATGACAAAAAGTCGTGGGGAATGCAAGTTCATATTGTTGTTAGTAGTTGTACCTGAGTCAATAACCCATGATACCTCAGATATTATACTAGGACCATTCAAGCATAGGAAATTGAAGTTATTACTAGTTGTGGCTAGAAAGCAAGTGTAAGAAGGTTTATTGAGAGAAACAATGAGGTCCTGTAGGTGCTCCAACTCAGTTATCAAGGTTGGTACATCCTTATTTGCATGCGACTTCACTATGGGATCAGAGTTTGAGGCAATCTGATTGATCTAAGTATTGAATGTTGTGGAAATGGTTGAACCTGAGCTGGTTTTCCTTGATGACTTGGTAGTTTCATTGTCATACACGTAGCACTAATCCAGTAATAGTGAGATCAAAGAAGCTTTGTTAGGAGAGTCAACCTTAACTTTGGGAAACGGCCGCATAGACGATGCAGATGAGCAAGTCGGGAATGCCATTGCGGTGAACATAGGAAGGACATGCCTCACATGATACCAAAAGAAGGCACGCAAGTCTGACTTTTAGGCAAACCACACGGCGCATATGATGGCAGCTGATGGCATGTCTCTAGTATTTGATCGAATTGGGGGATCACCTTGGTTGGTGCATTGATATGGTAGATGAGATGCATACACTTGGGAAGTCATTTGCAGGATCTACTTTCTTTGTGTGTTGGTGTGCTGATGACGTGGTTTGCTTTAAAGCTTTGTTGGTAACTAAAGTATGTTCAAACTGGGGTTCAGTTAGTAGATATACTGATTATAGTTTTTGTAGGAAGTAGGGTCAATGCTAATATGAATGCACACACACACAGATCACTGCTTTCAAATTTTTGTTTCTAATTCCATGTAACCAGCAGAATTTTGATATAGGATTGCAAGTTTTAATGTTCTCATTGATGTGACTGTTTGGAGCTTAGAGTACCTAAAAGTTTCAACTGATGCTCAATTGTGAATTTGCAGTTGGTGGCAAGATTGACCAAGGGAATAGTTGAAACATACCAAATATGCAATCCAAAGTTTAAATATTCTGATGATCTAAATCCCAAAAGATTTTTGACCAGCCCATCTACTGGAGTGCTTAATGATGGCTTTGATAATGCAAACTCGGATCTTATTCTGACAGTGAATTTGGTCTTGATCCATTTAGAGAAAAGTAGAAGGTTTGTCATACATATTTTGTTCCTTCAATACAATAATTATATATGTTCCTTTATGAATACCAAAGTGAATTTTCTGGGGATACCAAATGTGTCACACTAACCATCTGGCAAAATCACTACGTCTTCATATAATTTGCATTATGATATGGTGCAGATATATTGTCAAAGATCTCCTTGGCCATGGGACATTTGGACAAGTGGCCAAATGCTGGAATTCAGATACCAACAGTTTTGTTGCTGTGAAGATCATTAAAAATCAACCTGCATACTATCAACAGGCACTGGTTGAAGTAACTATTTTAACAACGGTAACCAGTTGTTGTTATTTTTATTTTAAGATGCTAGTCATTTTCTTGGAAGCACACACCCATGAGAAGCTTCTAAGGCACTCTTTTTATTTTTCTTCCTTTTTTTTTTTTTTTTTCCTGCAGTTAAATAAGAAGTACGATCCTGAGGATAAGCATCACATTGTTCGTATATATGATTATTTTGTATATCAACGGCATTTGTGCATATGCTTTGAACTTCTGGACACAAACCTGTAAGTTGCCAACTTCTTTGCCGTTTGATATTTGTCTGGCTTTCATCATTGAGAATGTATCAAAACTTGGATGATGTTCTAGTTCAACATAACTAAAATTCTTGGACGAACTATGCAATGGTGCTATCCTAGATTTGTTGTTTGCCTCCCTCTTGCATATTTTGGTATTGACATTAAATAGTATACAGGTATGAGCTTATCAAAATGAATCATTTTAGGGGATTGTCACTTGGTATTGTTCAGTTGTTCTCTAAGCAGGTATGTCAATTCTTTGTGTGGCCTTTCTGTTTAGTCCTTTTTGTTGGTTTTGTTATCTTACTATAATATGTTTTCTTAAATTTATGTTTTAAGATTTTATGTGGACTGGCTCTATTAAAAGATGCTGGCATTATTCATTGTGATCTGAAGCCAGAAAACATTCTTTTATGTACGAGGTTGGTTTCTAACATAAAGGTGCTCATAAAGAACTGTATGCTGCAGCTATTTTCGTTAACTTTTCATGTACGCTGCAGCTATTTCATCTTGAGATTTATGTAACCTTATTTTGTGCACAGTTCCGTGAAGTCAGCAGAAATTAAGATTATTGACTTTGGATCAGCATGCATGGAAAACCGTACTGTTTATTCATATATTCAGGTATACAATTACAATCTCTGTCGAGAGCGGGCTTTTACGATCATTCCTGTTGTAATTATAATTATCTGGTACTTACATTACACATCTTACCTGTTTTTTGTGCAGAGTCGATACTATAGATCTCCCGAGGTTCTTCTTGGATATCAGTATCCTAACCCATGA
Protein sequence

>MS.gene83689.t1

MDEVVRPVGSSCSSEVEHTQSRDTVVETASSSDSGWRPRELAFAPFVPRGDEQRVNAAGKSLKLRAFVRKPMLMANLCFNLTVPCIGDEMHTLGKSFAGSTFFVCWCADDVVCFKALLLVARLTKGIVETYQICNPKFKYSDDLNPKRFLTSPSTGVLNDGFDNANSDLILTVNLVLIHLEKSRRYIVKDLLGHGTFGQVAKCWNSDTNSFVAVKIIKNQPAYYQQALVEVTILTTLNKKYDPEDKHHIVRIYDYFVYQRHLCICFELLDTNLYELIKMNHFRGLSLGIVQLFSKQILCGLALLKDAGIIHCDLKPENILLCTSSVKSAEIKIIDFGSACMENRTVYSYIQSRYYRSPEVLLGYQYPNP