AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85553


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85553.t1 MTR_1g075990 93.103 174 6 3 17 189 13 181 5.06e-103 295
MS.gene85553.t1 MTR_1g442860 71.958 189 45 2 1 181 1 189 4.26e-89 260
MS.gene85553.t1 MTR_4g094428 71.975 157 34 2 36 192 31 177 1.19e-80 240
MS.gene85553.t1 MTR_5g072510 76.296 135 32 0 47 181 27 161 1.93e-77 231
MS.gene85553.t1 MTR_7g097030 86.861 137 14 1 53 189 60 192 9.75e-76 226
MS.gene85553.t1 MTR_1g069825 87.970 133 12 1 57 189 52 180 1.28e-74 224
MS.gene85553.t1 MTR_4g079930 66.013 153 47 1 25 177 2 149 2.88e-72 216
MS.gene85553.t1 MTR_2g016110 73.846 130 34 0 48 177 26 155 3.72e-71 214
MS.gene85553.t1 MTR_2g092950 77.419 124 28 0 54 177 20 143 4.84e-71 213
MS.gene85553.t1 MTR_2g076400 77.236 123 28 0 55 177 31 153 1.70e-70 212
MS.gene85553.t1 MTR_7g115700 62.112 161 51 3 30 189 5 156 7.63e-67 202
MS.gene85553.t1 MTR_3g031830 73.571 140 35 1 57 196 42 179 2.01e-66 203
MS.gene85553.t1 MTR_1g080210 81.600 125 23 0 57 181 40 164 4.18e-66 201
MS.gene85553.t1 MTR_8g086350 73.171 123 33 0 55 177 29 151 1.70e-55 174
MS.gene85553.t1 MTR_1g114020 73.077 52 14 0 49 100 63 114 4.81e-21 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85553.t1 AT3G23290 90.698 129 12 0 53 181 44 172 7.53e-78 231
MS.gene85553.t1 AT1G07090 70.946 148 39 1 47 194 25 168 1.59e-76 228
MS.gene85553.t1 AT2G31160 75.155 161 37 1 24 181 18 178 1.80e-75 226
MS.gene85553.t1 AT5G58500 74.815 135 29 2 56 189 18 148 3.21e-73 219
MS.gene85553.t1 AT5G28490 73.780 164 31 3 28 191 4 155 2.08e-69 210
MS.gene85553.t1 AT2G42610 69.286 140 39 1 38 177 10 145 4.25e-69 208
MS.gene85553.t1 AT2G42610 69.286 140 39 1 38 177 10 145 4.25e-69 208
MS.gene85553.t1 AT3G04510 78.519 135 25 1 57 191 33 163 3.15e-66 202
MS.gene85553.t1 AT1G78815 70.968 124 36 0 54 177 37 160 1.88e-64 197
MS.gene85553.t1 AT4G18610 69.919 123 36 1 56 177 36 158 2.48e-61 189
MS.gene85553.t1 AT1G16910 61.429 140 50 1 54 193 20 155 5.28e-59 182

Find 34 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTACATGCTTCTTCTGTTT+AGG 0.245569 1.3:+54447295 None:intergenic
GTACATGCTTCTTCTGTTTA+GGG 0.251992 1.3:+54447296 None:intergenic
AGAAGAGACTGGAACACTTT+TGG 0.322091 1.3:-54448370 MS.gene85553:CDS
AGAGTGTTTGTGTTTGTTGC+TGG 0.335361 1.3:+54448415 None:intergenic
TTAAGGTATCTTGATCAGTT+TGG 0.338884 1.3:-54448277 MS.gene85553:CDS
TAAGGTATCTTGATCAGTTT+GGG 0.342770 1.3:-54448276 MS.gene85553:CDS
GACTCTTTCTTCGTGAAGTT+AGG 0.348358 1.3:-54448072 MS.gene85553:CDS
GGACAAGGTGCAGGTGGGTT+TGG 0.354086 1.3:+54448211 None:intergenic
TTGTCCTCTCAGACAAGCTT+GGG 0.367576 1.3:-54448188 MS.gene85553:CDS
GGAAGGATACACTTTGGAAT+TGG 0.368060 1.3:-54447323 MS.gene85553:intron
AAACCTGAAGATAACCCTTT+TGG 0.390636 1.3:-54448109 MS.gene85553:CDS
GCACCAAAAGGGTTATCTTC+AGG 0.403413 1.3:+54448106 None:intergenic
TTCTGAGCTGGTTGATGTGT+TGG 0.404733 1.3:+54448486 None:intergenic
ACTCTTTCTTCGTGAAGTTA+GGG 0.427767 1.3:-54448071 MS.gene85553:CDS
CTTGTCCTCTCAGACAAGCT+TGG 0.437451 1.3:-54448189 MS.gene85553:CDS
TGATCAGTTTGGGAAAACTA+AGG 0.457631 1.3:-54448266 MS.gene85553:CDS
GGAAGTCTTGATGCATTGAT+TGG 0.461550 1.3:-54448166 MS.gene85553:CDS
ACAGAAGAAGCATGTACTCT+TGG 0.477980 1.3:-54447290 MS.gene85553:CDS
CTAACAGCACGAGCACCAAA+AGG 0.492363 1.3:+54448094 None:intergenic
AGAGTGAAAGTGGTGGTCTA+TGG 0.493036 1.3:+54448332 None:intergenic
CTTTCACTCTCACGTTGTAG+TGG 0.531680 1.3:-54448319 MS.gene85553:CDS
GTGTTTGTGTTTGTTGCTGG+TGG 0.560971 1.3:+54448418 None:intergenic
AGAGGACAAGGACAAGGTGC+AGG 0.565916 1.3:+54448202 None:intergenic
CTATTGGGAAGGATACACTT+TGG 0.570184 1.3:-54447329 MS.gene85553:intron
CAAGCTTGTCTGAGAGGACA+AGG 0.577230 1.3:+54448190 None:intergenic
CTACAACGTGAGAGTGAAAG+TGG 0.580478 1.3:+54448322 None:intergenic
GGACAAGGACAAGGTGCAGG+TGG 0.580568 1.3:+54448205 None:intergenic
CTTCCCCAAGCTTGTCTGAG+AGG 0.586547 1.3:+54448184 None:intergenic
TAACAGCACGAGCACCAAAA+GGG 0.587993 1.3:+54448095 None:intergenic
GACTCTCAATCTAAAGCTAG+AGG 0.600860 1.3:-54448049 MS.gene85553:intron
GACAAGGACAAGGTGCAGGT+GGG 0.650602 1.3:+54448206 None:intergenic
TGTCCTCTCAGACAAGCTTG+GGG 0.658773 1.3:-54448187 MS.gene85553:CDS
TGTCTGAGAGGACAAGGACA+AGG 0.664143 1.3:+54448196 None:intergenic
CAACGTGAGAGTGAAAGTGG+TGG 0.746060 1.3:+54448325 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGAAACAAATATATATTTT+TGG + chr1.3:54447966-54447985 None:intergenic 10.0%
!!! TATAACTTTTAACAAATTTA+AGG + chr1.3:54448028-54448047 None:intergenic 10.0%
!!! TTAAATTTAGAGGTTATTTT+TGG + chr1.3:54448414-54448433 None:intergenic 15.0%
!! TGAATAGCATTTAAATTTAG+AGG + chr1.3:54448424-54448443 None:intergenic 20.0%
!!! AATCATTAACAACAGTTTTT+TGG - chr1.3:54448243-54448262 MS.gene85553:CDS 20.0%
!!! TCATTAACCACAAAATTTTT+AGG + chr1.3:54448295-54448314 None:intergenic 20.0%
! ACTAATTAAGTACTAAGCAA+TGG - chr1.3:54447876-54447895 MS.gene85553:intron 25.0%
! ACTTAATTAGTTGCATTTGA+TGG + chr1.3:54447867-54447886 None:intergenic 25.0%
! ATTAACACGATTCAAATACA+CGG - chr1.3:54448164-54448183 MS.gene85553:CDS 25.0%
! TTGAAGTTTCAACTATATGA+GGG - chr1.3:54448050-54448069 MS.gene85553:CDS 25.0%
! TTTGCTAATACTTGCATATT+TGG + chr1.3:54448103-54448122 None:intergenic 25.0%
!! ATTTTGTGGTTAATGAAGAT+AGG - chr1.3:54448299-54448318 MS.gene85553:CDS 25.0%
!! CACATGTTCTTGAATTTTTA+AGG - chr1.3:54447541-54447560 MS.gene85553:intron 25.0%
!! TTGAACACCTAAAAATTTTG+TGG - chr1.3:54448285-54448304 MS.gene85553:CDS 25.0%
!! TTTGAAGTTTCAACTATATG+AGG - chr1.3:54448049-54448068 MS.gene85553:intron 25.0%
AGAATCAAAAAAGAAGAGAC+TGG - chr1.3:54447454-54447473 MS.gene85553:intron 30.0%
TAATTAGTTGCATTTGATGG+TGG + chr1.3:54447864-54447883 None:intergenic 30.0%
! TAAGGTATCTTGATCAGTTT+GGG - chr1.3:54447559-54447578 MS.gene85553:intron 30.0%
! TTAAGGTATCTTGATCAGTT+TGG - chr1.3:54447558-54447577 MS.gene85553:intron 30.0%
!!! TCACAGATTTGTCCATTTTT+TGG - chr1.3:54447597-54447616 MS.gene85553:intron 30.0%
ACTCTTTCTTCGTGAAGTTA+GGG - chr1.3:54447764-54447783 MS.gene85553:intron 35.0%
AGTACATGCTTCTTCTGTTT+AGG + chr1.3:54448543-54448562 None:intergenic 35.0%
GTACATGCTTCTTCTGTTTA+GGG + chr1.3:54448542-54448561 None:intergenic 35.0%
TGATCAGTTTGGGAAAACTA+AGG - chr1.3:54447569-54447588 MS.gene85553:intron 35.0%
! AAACCTGAAGATAACCCTTT+TGG - chr1.3:54447726-54447745 MS.gene85553:intron 35.0%
! GGTTAATGAAGATAGGTTGA+TGG - chr1.3:54448306-54448325 MS.gene85553:CDS 35.0%
!! TCAACTATATGAGGGTGTTA+GGG - chr1.3:54448058-54448077 MS.gene85553:CDS 35.0%
!! TTCAACTATATGAGGGTGTT+AGG - chr1.3:54448057-54448076 MS.gene85553:CDS 35.0%
!!! TGGTTACTAGTTTTGACACA+TGG - chr1.3:54448326-54448345 MS.gene85553:CDS 35.0%
ATACTGAGTGACTGCACTAT+TGG - chr1.3:54448490-54448509 MS.gene85553:CDS 40.0%
CTATTGGGAAGGATACACTT+TGG - chr1.3:54448506-54448525 MS.gene85553:CDS 40.0%
GACTCTTTCTTCGTGAAGTT+AGG - chr1.3:54447763-54447782 MS.gene85553:intron 40.0%
GGGTTTGGATGACCAAAAAA+TGG + chr1.3:54447612-54447631 None:intergenic 40.0%
TACTGAGTGACTGCACTATT+GGG - chr1.3:54448491-54448510 MS.gene85553:CDS 40.0%
! ACAGAAGAAGCATGTACTCT+TGG - chr1.3:54448545-54448564 MS.gene85553:CDS 40.0%
! AGAAGAGACTGGAACACTTT+TGG - chr1.3:54447465-54447484 MS.gene85553:intron 40.0%
! AGAGTGTTTGTGTTTGTTGC+TGG + chr1.3:54447423-54447442 None:intergenic 40.0%
! GACTCTCAATCTAAAGCTAG+AGG - chr1.3:54447786-54447805 MS.gene85553:intron 40.0%
! GGAAGTCTTGATGCATTGAT+TGG - chr1.3:54447669-54447688 MS.gene85553:intron 40.0%
!! CTGCTTTTGAAGAGAATGGT+GGG - chr1.3:54447703-54447722 MS.gene85553:intron 40.0%
!! GGAAGGATACACTTTGGAAT+TGG - chr1.3:54448512-54448531 MS.gene85553:CDS 40.0%
!! GTTTTTGTTGTCTTCTGAGC+TGG + chr1.3:54447364-54447383 None:intergenic 40.0%
!! TTAGTTGCATTTGATGGTGG+TGG + chr1.3:54447861-54447880 None:intergenic 40.0%
!!! AGAGCTGCTTTTGAAGAGAA+TGG - chr1.3:54447699-54447718 MS.gene85553:intron 40.0%
AGAGTGAAAGTGGTGGTCTA+TGG + chr1.3:54447506-54447525 None:intergenic 45.0%
CTACAACGTGAGAGTGAAAG+TGG + chr1.3:54447516-54447535 None:intergenic 45.0%
CTTTCACTCTCACGTTGTAG+TGG - chr1.3:54447516-54447535 MS.gene85553:intron 45.0%
GCACCAAAAGGGTTATCTTC+AGG + chr1.3:54447732-54447751 None:intergenic 45.0%
TAACAGCACGAGCACCAAAA+GGG + chr1.3:54447743-54447762 None:intergenic 45.0%
TTGTCCTCTCAGACAAGCTT+GGG - chr1.3:54447647-54447666 MS.gene85553:intron 45.0%
! GTGTTTGTGTTTGTTGCTGG+TGG + chr1.3:54447420-54447439 None:intergenic 45.0%
! TTCTGAGCTGGTTGATGTGT+TGG + chr1.3:54447352-54447371 None:intergenic 45.0%
!!! GCTGCTTTTGAAGAGAATGG+TGG - chr1.3:54447702-54447721 MS.gene85553:intron 45.0%
CAACGTGAGAGTGAAAGTGG+TGG + chr1.3:54447513-54447532 None:intergenic 50.0%
CAAGCTTGTCTGAGAGGACA+AGG + chr1.3:54447648-54447667 None:intergenic 50.0%
CTAACAGCACGAGCACCAAA+AGG + chr1.3:54447744-54447763 None:intergenic 50.0%
CTTGTCCTCTCAGACAAGCT+TGG - chr1.3:54447646-54447665 MS.gene85553:intron 50.0%
GAGTGACTGCACTATTGGGA+AGG - chr1.3:54448495-54448514 MS.gene85553:CDS 50.0%
TGTCCTCTCAGACAAGCTTG+GGG - chr1.3:54447648-54447667 MS.gene85553:intron 50.0%
TGTCTGAGAGGACAAGGACA+AGG + chr1.3:54447642-54447661 None:intergenic 50.0%
!! GGTGGTGGTGGTTGAATTTG+AGG + chr1.3:54447843-54447862 None:intergenic 50.0%
!! GTTGCATTTGATGGTGGTGG+TGG + chr1.3:54447858-54447877 None:intergenic 50.0%
AGAGGACAAGGACAAGGTGC+AGG + chr1.3:54447636-54447655 None:intergenic 55.0%
CTTCCCCAAGCTTGTCTGAG+AGG + chr1.3:54447654-54447673 None:intergenic 55.0%
GACAAGGACAAGGTGCAGGT+GGG + chr1.3:54447632-54447651 None:intergenic 55.0%
!! GCATTTGATGGTGGTGGTGG+TGG + chr1.3:54447855-54447874 None:intergenic 55.0%
GGACAAGGACAAGGTGCAGG+TGG + chr1.3:54447633-54447652 None:intergenic 60.0%
! GGACAAGGTGCAGGTGGGTT+TGG + chr1.3:54447627-54447646 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 54447279 54448578 54447279 ID=MS.gene85553
chr1.3 mRNA 54447279 54448578 54447279 ID=MS.gene85553.t1;Parent=MS.gene85553
chr1.3 exon 54448050 54448578 54448050 ID=MS.gene85553.t1.exon1;Parent=MS.gene85553.t1
chr1.3 CDS 54448050 54448578 54448050 ID=cds.MS.gene85553.t1;Parent=MS.gene85553.t1
chr1.3 exon 54447279 54447340 54447279 ID=MS.gene85553.t1.exon2;Parent=MS.gene85553.t1
chr1.3 CDS 54447279 54447340 54447279 ID=cds.MS.gene85553.t1;Parent=MS.gene85553.t1
Gene Sequence

>MS.gene85553

ATGAATTCATTTGAAGAATTCAACTCATCATCATCAACAAACACACAAACAAAAAACATCATCAACTTCACCAACACATCAACCAGCTCAGAAGACAACAAAAACATCTCAAATTTCACATCTTCTTCTTCAGCAGTACCACCAGCAACAAACACAAACACTCTAAGTCGCTATGAGAATCAAAAAAGAAGAGACTGGAACACTTTTGGTCAATACTTAAGAAACCATAGACCACCACTTTCACTCTCACGTTGTAGTGGAGCACATGTTCTTGAATTTTTAAGGTATCTTGATCAGTTTGGGAAAACTAAGGTTCACTCACAGATTTGTCCATTTTTTGGTCATCCAAACCCACCTGCACCTTGTCCTTGTCCTCTCAGACAAGCTTGGGGAAGTCTTGATGCATTGATTGGAAGACTAAGAGCTGCTTTTGAAGAGAATGGTGGGAAACCTGAAGATAACCCTTTTGGTGCTCGTGCTGTTAGACTCTTTCTTCGTGAAGTTAGGGACTCTCAATCTAAAGCTAGAGGTATTAGCTATGAGAAAAAGAAGCGTAAAAGACCTCAAATTCAACCACCACCACCACCATCAAATGCAACTAATTAAGTACTAAGCAATGGTATATTATATATATTATTCTCTCATATTTAATTAGCATGTAACAAGTTGTTTCATTCTGTTGTTCCAAAAATATATATTTGTTTCATTCATTCTAGCAGTGAGTTTAGATAAATCACAGTCAAACACCTTAAATTTGTTAAAAGTTATATTTTGAAGTTTCAACTATATGAGGGTGTTAGGGTATGTGATTTTGTGACATTCCAAATATGCAAGTATTAGCAAATATGTGTGTTTGAACAATCAAACTTAAACAATTTTTAAGTGATTAACACGATTCAAATACACGGTTAATTTTATATTTATAGTAGATGATTAATGAATATAACATGTGTCTAAAGTTATTAATCATTAACAACAGTTTTTTGGTGTTCAAATCTACTTGCAATTGAACACCTAAAAATTTTGTGGTTAATGAAGATAGGTTGATGGTTACTAGTTTTGACACATGGTATATTCATTAATAATCTAGTAAAAATTAGTCACTTATTTGCACTGTGTAAATTGTAGTTCTCCAAAAATAACCTCTAAATTTAAATGCTATTCATTTTTTACTTTTATATTTGCTAGTTGTGTGCAGAAGTTGTGATTTGATACTGAGTGACTGCACTATTGGGAAGGATACACTTTGGAATTGGAATACCCTAAACAGAAGAAGCATGTACTCTTGGTTGAAGCATGA

Protein sequence

>MS.gene85553.t1

MNSFEEFNSSSSTNTQTKNIINFTNTSTSSEDNKNISNFTSSSSAVPPATNTNTLSRYENQKRRDWNTFGQYLRNHRPPLSLSRCSGAHVLEFLRYLDQFGKTKVHSQICPFFGHPNPPAPCPCPLRQAWGSLDALIGRLRAAFEENGGKPEDNPFGARAVRLFLREVRDSQSKARGYTLELEYPKQKKHVLLVEA