Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88977.t1 | XP_003589562.1 | 91.7 | 254 | 14 | 2 | 1 | 254 | 1 | 247 | 1.60E-128 | 468.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88977.t1 | Q9SGS5 | 31.6 | 285 | 132 | 9 | 4 | 250 | 13 | 272 | 6.3e-20 | 99.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88977.t1 | G7I8D2 | 91.7 | 254 | 14 | 2 | 1 | 254 | 1 | 247 | 1.2e-128 | 468.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049181 | MS.gene88977 | 0.847632 | 1.02E-59 | -1.69E-46 |
| MS.gene049457 | MS.gene88977 | 0.815122 | 1.09E-51 | -1.69E-46 |
| MS.gene050363 | MS.gene88977 | 0.818496 | 1.90E-52 | -1.69E-46 |
| MS.gene050461 | MS.gene88977 | 0.805865 | 1.09E-49 | -1.69E-46 |
| MS.gene050526 | MS.gene88977 | 0.82264 | 2.13E-53 | -1.69E-46 |
| MS.gene050883 | MS.gene88977 | 0.815857 | 7.46E-52 | -1.69E-46 |
| MS.gene051959 | MS.gene88977 | 0.82604 | 3.37E-54 | -1.69E-46 |
| MS.gene051960 | MS.gene88977 | 0.825245 | 5.21E-54 | -1.69E-46 |
| MS.gene052053 | MS.gene88977 | 0.812124 | 4.96E-51 | -1.69E-46 |
| MS.gene052091 | MS.gene88977 | 0.850491 | 1.64E-60 | -1.69E-46 |
| MS.gene053826 | MS.gene88977 | 0.828309 | 9.66E-55 | -1.69E-46 |
| MS.gene053932 | MS.gene88977 | 0.851818 | 6.93E-61 | -1.69E-46 |
| MS.gene055549 | MS.gene88977 | 0.864821 | 9.23E-65 | -1.69E-46 |
| MS.gene055550 | MS.gene88977 | 0.868614 | 5.73E-66 | -1.69E-46 |
| MS.gene055551 | MS.gene88977 | 0.864224 | 1.42E-64 | -1.69E-46 |
| MS.gene056311 | MS.gene88977 | 0.812965 | 3.25E-51 | -1.69E-46 |
| MS.gene056365 | MS.gene88977 | 0.809918 | 1.49E-50 | -1.69E-46 |
| MS.gene056408 | MS.gene88977 | 0.823771 | 1.16E-53 | -1.69E-46 |
| MS.gene056530 | MS.gene88977 | 0.847455 | 1.14E-59 | -1.69E-46 |
| MS.gene057001 | MS.gene88977 | 0.806874 | 6.66E-50 | -1.69E-46 |
| MS.gene057093 | MS.gene88977 | 0.827958 | 1.17E-54 | -1.69E-46 |
| MS.gene058628 | MS.gene88977 | 0.83486 | 2.35E-56 | -1.69E-46 |
| MS.gene058629 | MS.gene88977 | 0.836946 | 6.94E-57 | -1.69E-46 |
| MS.gene058630 | MS.gene88977 | 0.837928 | 3.89E-57 | -1.69E-46 |
| MS.gene060446 | MS.gene88977 | 0.810589 | 1.07E-50 | -1.69E-46 |
| MS.gene060964 | MS.gene88977 | 0.812682 | 3.75E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88977.t1 | MTR_1g026110 | 91.732 | 254 | 14 | 2 | 1 | 254 | 1 | 247 | 2.43e-163 | 452 |
| MS.gene88977.t1 | MTR_3g114750 | 48.485 | 264 | 98 | 11 | 4 | 250 | 2 | 244 | 3.07e-59 | 188 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88977.t1 | AT1G76070 | 33.212 | 274 | 120 | 10 | 15 | 250 | 24 | 272 | 2.80e-28 | 108 |
| MS.gene88977.t1 | AT1G20310 | 34.686 | 271 | 120 | 11 | 1 | 247 | 1 | 238 | 3.51e-27 | 105 |
Find 61 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAGATCTTTGTTGGGTTT+TGG | 0.236347 | 1.4:-304049 | None:intergenic |
| TCCCTTTGATCAATTTCTTC+AGG | 0.304197 | 1.4:-304295 | None:intergenic |
| TTCACCAAAGATTTCTTGTA+TGG | 0.307383 | 1.4:+303994 | MS.gene88977:CDS |
| TCATTGCCACCACCATCTTT+AGG | 0.317528 | 1.4:-303956 | None:intergenic |
| CCTAAAATAGGTGCAGAAAA+TGG | 0.326900 | 1.4:-304355 | None:intergenic |
| TTTCCACCAACACCTAAAAT+AGG | 0.335034 | 1.4:-304367 | None:intergenic |
| CTAAATTTGTGATCTCTTCC+TGG | 0.339147 | 1.4:-303830 | None:intergenic |
| TTTACCTCCGTATCCACCTT+TGG | 0.375428 | 1.4:-304091 | None:intergenic |
| GAAAGGAAATCAGATGGTTC+TGG | 0.386986 | 1.4:+304166 | MS.gene88977:CDS |
| CAAGAAATCTTTGGTGAAGT+AGG | 0.393822 | 1.4:-303989 | None:intergenic |
| CAAGGAATGAGATTAATCTT+TGG | 0.407911 | 1.4:+304428 | MS.gene88977:CDS |
| TTAGGTGTTGGTGGAAATAG+TGG | 0.423953 | 1.4:+304373 | MS.gene88977:CDS |
| TGTCCCATACAAGAAATCTT+TGG | 0.426848 | 1.4:-303998 | None:intergenic |
| GCCTGAAGAAATTGATCAAA+GGG | 0.432040 | 1.4:+304294 | MS.gene88977:CDS |
| GGAAGTGAAGAGATCTTTGT+TGG | 0.442034 | 1.4:-304056 | None:intergenic |
| CAAAGATTAATCTCATTCCT+TGG | 0.442885 | 1.4:-304427 | None:intergenic |
| CAAAACGCTCCTTTCAGTCC+AGG | 0.469371 | 1.4:+303812 | MS.gene88977:CDS |
| AGAAAATAACACAAAGTCAT+GGG | 0.481776 | 1.4:+303856 | MS.gene88977:CDS |
| GGTTCTGGATTTGTTGATGA+AGG | 0.482613 | 1.4:+304181 | MS.gene88977:CDS |
| CACTCCACCAAAGGTGGATA+CGG | 0.483600 | 1.4:+304084 | MS.gene88977:CDS |
| CAAAGGGATTGCTTTACTGA+TGG | 0.491176 | 1.4:+304310 | MS.gene88977:CDS |
| ACCTCCGTATCCACCTTTGG+TGG | 0.491667 | 1.4:-304088 | None:intergenic |
| CACCGCCGATGGGTGATATG+AGG | 0.497283 | 1.4:+304215 | MS.gene88977:CDS |
| GGATTCAACTGAAGAGGCCT+AGG | 0.502469 | 1.4:-304472 | None:intergenic |
| ATTTATTTAGCTTTAAGCAC+AGG | 0.503675 | 1.4:-304493 | None:intergenic |
| AAAACTCATGCTACTGGTAA+GGG | 0.508213 | 1.4:+303884 | MS.gene88977:CDS |
| CGTATCCACCTTTGGTGGAG+TGG | 0.514538 | 1.4:-304083 | None:intergenic |
| CTGATTTGAAGTTTAAGCCA+AGG | 0.518893 | 1.4:+304410 | MS.gene88977:CDS |
| TGATCTCTTCCTGGACTGAA+AGG | 0.521071 | 1.4:-303821 | None:intergenic |
| CAGAAAATAACACAAAGTCA+TGG | 0.527453 | 1.4:+303855 | MS.gene88977:CDS |
| AAGAGAAACAAAGTAAGCTA+AGG | 0.530956 | 1.4:+303756 | MS.gene88977:CDS |
| GCTAGAAGAAAACCTAAAGA+TGG | 0.536495 | 1.4:+303944 | MS.gene88977:CDS |
| GAAGTGAAGAGATCTTTGTT+GGG | 0.537217 | 1.4:-304055 | None:intergenic |
| CACCTTTGGTGGAGTGGACA+CGG | 0.538901 | 1.4:-304077 | None:intergenic |
| AGCCTGAAGAAATTGATCAA+AGG | 0.541841 | 1.4:+304293 | MS.gene88977:CDS |
| TAAAGAGATCGAAGTTTCAG+AGG | 0.548970 | 1.4:+304110 | MS.gene88977:CDS |
| CGTGTCCACTCCACCAAAGG+TGG | 0.560517 | 1.4:+304078 | MS.gene88977:CDS |
| AACAACAAAGCACCGCCGAT+GGG | 0.567253 | 1.4:+304205 | MS.gene88977:CDS |
| AGCACAGGATTCAACTGAAG+AGG | 0.568748 | 1.4:-304478 | None:intergenic |
| TCACCAAAGATTTCTTGTAT+GGG | 0.570550 | 1.4:+303995 | MS.gene88977:CDS |
| TTCCGTGTCCACTCCACCAA+AGG | 0.573551 | 1.4:+304075 | MS.gene88977:CDS |
| GTTCTGGATTTGTTGATGAA+GGG | 0.581024 | 1.4:+304182 | MS.gene88977:CDS |
| AGAAAACCTAAAGATGGTGG+TGG | 0.587690 | 1.4:+303950 | MS.gene88977:CDS |
| TATGGAATCATAGTAACCAT+AGG | 0.589627 | 1.4:-303917 | None:intergenic |
| GAACAACAAAGCACCGCCGA+TGG | 0.591209 | 1.4:+304204 | MS.gene88977:CDS |
| GAGATTTGCAAGTGGACGTG+AGG | 0.598149 | 1.4:+304237 | MS.gene88977:CDS |
| TTTGCGGAAAGGAAATCAGA+TGG | 0.598533 | 1.4:+304160 | MS.gene88977:CDS |
| TTGCTTTACTGATGGAGAGG+AGG | 0.603818 | 1.4:+304318 | MS.gene88977:CDS |
| GGATTGCTTTACTGATGGAG+AGG | 0.611817 | 1.4:+304315 | MS.gene88977:CDS |
| CTAAAGCGAAATTTGCGGAA+AGG | 0.612185 | 1.4:+304149 | MS.gene88977:CDS |
| GAAGAACAATGGCTCCACCT+AGG | 0.627085 | 1.4:+304455 | MS.gene88977:CDS |
| TTCAACTGAAGAGGCCTAGG+TGG | 0.634353 | 1.4:-304469 | None:intergenic |
| AGAAGAAAACCTAAAGATGG+TGG | 0.634473 | 1.4:+303947 | MS.gene88977:CDS |
| TCTTTGGAAGAGAAGAACAA+TGG | 0.638654 | 1.4:+304444 | MS.gene88977:CDS |
| AATCTCCTCATATCACCCAT+CGG | 0.643282 | 1.4:-304220 | None:intergenic |
| GAAAATAACACAAAGTCATG+GGG | 0.645047 | 1.4:+303857 | MS.gene88977:CDS |
| AAATAGTGGTACATCTAATG+TGG | 0.671533 | 1.4:+304387 | MS.gene88977:CDS |
| TCCACCAAAGGTGGATACGG+AGG | 0.678054 | 1.4:+304087 | MS.gene88977:CDS |
| CTCCTCATATCACCCATCGG+CGG | 0.680805 | 1.4:-304217 | None:intergenic |
| GAGGTCTAAAGCGAAATTTG+CGG | 0.681767 | 1.4:+304144 | MS.gene88977:CDS |
| GATATGAGGAGATTTGCAAG+TGG | 0.723470 | 1.4:+304229 | MS.gene88977:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTCTAAATTTGATTGGA+AGG | + | chr1.4:304261-304280 | MS.gene88977:CDS | 20.0% |
| ! | AGAAAATAACACAAAGTCAT+GGG | + | chr1.4:303856-303875 | MS.gene88977:CDS | 25.0% |
| !!! | AGGCTTTTTCTAAATTTGAT+TGG | + | chr1.4:304257-304276 | MS.gene88977:CDS | 25.0% |
| AAATAGTGGTACATCTAATG+TGG | + | chr1.4:304387-304406 | MS.gene88977:CDS | 30.0% | |
| AAGAGAAACAAAGTAAGCTA+AGG | + | chr1.4:303756-303775 | MS.gene88977:CDS | 30.0% | |
| CAAAGATTAATCTCATTCCT+TGG | - | chr1.4:304430-304449 | None:intergenic | 30.0% | |
| CAAGGAATGAGATTAATCTT+TGG | + | chr1.4:304428-304447 | MS.gene88977:CDS | 30.0% | |
| CAGAAAATAACACAAAGTCA+TGG | + | chr1.4:303855-303874 | MS.gene88977:CDS | 30.0% | |
| GAAAATAACACAAAGTCATG+GGG | + | chr1.4:303857-303876 | MS.gene88977:CDS | 30.0% | |
| TATGGAATCATAGTAACCAT+AGG | - | chr1.4:303920-303939 | None:intergenic | 30.0% | |
| TCACCAAAGATTTCTTGTAT+GGG | + | chr1.4:303995-304014 | MS.gene88977:CDS | 30.0% | |
| TTCACCAAAGATTTCTTGTA+TGG | + | chr1.4:303994-304013 | MS.gene88977:CDS | 30.0% | |
| AAAAACTCATGCTACTGGTA+AGG | + | chr1.4:303883-303902 | MS.gene88977:CDS | 35.0% | |
| AAAACTCATGCTACTGGTAA+GGG | + | chr1.4:303884-303903 | MS.gene88977:CDS | 35.0% | |
| AGAAGAAAACCTAAAGATGG+TGG | + | chr1.4:303947-303966 | MS.gene88977:CDS | 35.0% | |
| AGCCTGAAGAAATTGATCAA+AGG | + | chr1.4:304293-304312 | MS.gene88977:CDS | 35.0% | |
| CAAGAAATCTTTGGTGAAGT+AGG | - | chr1.4:303992-304011 | None:intergenic | 35.0% | |
| CCTAAAATAGGTGCAGAAAA+TGG | - | chr1.4:304358-304377 | None:intergenic | 35.0% | |
| CTAAATTTGTGATCTCTTCC+TGG | - | chr1.4:303833-303852 | None:intergenic | 35.0% | |
| GAAGTGAAGAGATCTTTGTT+GGG | - | chr1.4:304058-304077 | None:intergenic | 35.0% | |
| GCCTGAAGAAATTGATCAAA+GGG | + | chr1.4:304294-304313 | MS.gene88977:CDS | 35.0% | |
| GCTAGAAGAAAACCTAAAGA+TGG | + | chr1.4:303944-303963 | MS.gene88977:CDS | 35.0% | |
| TAAAGAGATCGAAGTTTCAG+AGG | + | chr1.4:304110-304129 | MS.gene88977:CDS | 35.0% | |
| TCCCTTTGATCAATTTCTTC+AGG | - | chr1.4:304298-304317 | None:intergenic | 35.0% | |
| TGTCCCATACAAGAAATCTT+TGG | - | chr1.4:304001-304020 | None:intergenic | 35.0% | |
| TTTCCACCAACACCTAAAAT+AGG | - | chr1.4:304370-304389 | None:intergenic | 35.0% | |
| ! | AAGAGATCTTTGTTGGGTTT+TGG | - | chr1.4:304052-304071 | None:intergenic | 35.0% |
| ! | CCATTTTCTGCACCTATTTT+AGG | + | chr1.4:304355-304374 | MS.gene88977:CDS | 35.0% |
| ! | CTGATTTGAAGTTTAAGCCA+AGG | + | chr1.4:304410-304429 | MS.gene88977:CDS | 35.0% |
| ! | TCTTTGGAAGAGAAGAACAA+TGG | + | chr1.4:304444-304463 | MS.gene88977:CDS | 35.0% |
| !! | GTTCTGGATTTGTTGATGAA+GGG | + | chr1.4:304182-304201 | MS.gene88977:CDS | 35.0% |
| !!! | GGTTTTCTTCTAGCTTCATA+TGG | - | chr1.4:303938-303957 | None:intergenic | 35.0% |
| AATCTCCTCATATCACCCAT+CGG | - | chr1.4:304223-304242 | None:intergenic | 40.0% | |
| CAAAGGGATTGCTTTACTGA+TGG | + | chr1.4:304310-304329 | MS.gene88977:CDS | 40.0% | |
| CTAAAGCGAAATTTGCGGAA+AGG | + | chr1.4:304149-304168 | MS.gene88977:CDS | 40.0% | |
| GAAAGGAAATCAGATGGTTC+TGG | + | chr1.4:304166-304185 | MS.gene88977:CDS | 40.0% | |
| GATATGAGGAGATTTGCAAG+TGG | + | chr1.4:304229-304248 | MS.gene88977:CDS | 40.0% | |
| GGAAGTGAAGAGATCTTTGT+TGG | - | chr1.4:304059-304078 | None:intergenic | 40.0% | |
| TTTGCGGAAAGGAAATCAGA+TGG | + | chr1.4:304160-304179 | MS.gene88977:CDS | 40.0% | |
| ! | AGAAAACCTAAAGATGGTGG+TGG | + | chr1.4:303950-303969 | MS.gene88977:CDS | 40.0% |
| ! | GAGGTCTAAAGCGAAATTTG+CGG | + | chr1.4:304144-304163 | MS.gene88977:CDS | 40.0% |
| ! | TAAGGGATTTTCTGGACCTA+TGG | + | chr1.4:303901-303920 | MS.gene88977:CDS | 40.0% |
| !! | GGTTCTGGATTTGTTGATGA+AGG | + | chr1.4:304181-304200 | MS.gene88977:CDS | 40.0% |
| !! | TCTGCACCTATTTTAGGTGT+TGG | + | chr1.4:304361-304380 | MS.gene88977:CDS | 40.0% |
| !! | TTAGGTGTTGGTGGAAATAG+TGG | + | chr1.4:304373-304392 | MS.gene88977:CDS | 40.0% |
| !!! | TTCAGAGGATTTTTTCGCAG+AGG | + | chr1.4:304125-304144 | MS.gene88977:CDS | 40.0% |
| AGCACAGGATTCAACTGAAG+AGG | - | chr1.4:304481-304500 | None:intergenic | 45.0% | |
| GGATTGCTTTACTGATGGAG+AGG | + | chr1.4:304315-304334 | MS.gene88977:CDS | 45.0% | |
| GGTGCAAAAACTCATGCTAC+TGG | + | chr1.4:303878-303897 | MS.gene88977:CDS | 45.0% | |
| TCATTGCCACCACCATCTTT+AGG | - | chr1.4:303959-303978 | None:intergenic | 45.0% | |
| TGATCTCTTCCTGGACTGAA+AGG | - | chr1.4:303824-303843 | None:intergenic | 45.0% | |
| TTGCTTTACTGATGGAGAGG+AGG | + | chr1.4:304318-304337 | MS.gene88977:CDS | 45.0% | |
| TTTACCTCCGTATCCACCTT+TGG | - | chr1.4:304094-304113 | None:intergenic | 45.0% | |
| ! | GCTACTGGTAAGGGATTTTC+TGG | + | chr1.4:303893-303912 | MS.gene88977:CDS | 45.0% |
| !!! | GCACCTATTTTAGGTGTTGG+TGG | + | chr1.4:304364-304383 | MS.gene88977:CDS | 45.0% |
| CAAAACGCTCCTTTCAGTCC+AGG | + | chr1.4:303812-303831 | MS.gene88977:CDS | 50.0% | |
| CACTCCACCAAAGGTGGATA+CGG | + | chr1.4:304084-304103 | MS.gene88977:CDS | 50.0% | |
| GAAGAACAATGGCTCCACCT+AGG | + | chr1.4:304455-304474 | MS.gene88977:CDS | 50.0% | |
| GAGATTTGCAAGTGGACGTG+AGG | + | chr1.4:304237-304256 | MS.gene88977:CDS | 50.0% | |
| GGATTCAACTGAAGAGGCCT+AGG | - | chr1.4:304475-304494 | None:intergenic | 50.0% | |
| TTCAACTGAAGAGGCCTAGG+TGG | - | chr1.4:304472-304491 | None:intergenic | 50.0% | |
| !! | AACAACAAAGCACCGCCGAT+GGG | + | chr1.4:304205-304224 | MS.gene88977:CDS | 50.0% |
| ACCTCCGTATCCACCTTTGG+TGG | - | chr1.4:304091-304110 | None:intergenic | 55.0% | |
| CACCTTTGGTGGAGTGGACA+CGG | - | chr1.4:304080-304099 | None:intergenic | 55.0% | |
| CGTATCCACCTTTGGTGGAG+TGG | - | chr1.4:304086-304105 | None:intergenic | 55.0% | |
| CTCCTCATATCACCCATCGG+CGG | - | chr1.4:304220-304239 | None:intergenic | 55.0% | |
| TCCACCAAAGGTGGATACGG+AGG | + | chr1.4:304087-304106 | MS.gene88977:CDS | 55.0% | |
| TTCCGTGTCCACTCCACCAA+AGG | + | chr1.4:304075-304094 | MS.gene88977:CDS | 55.0% | |
| !! | GAACAACAAAGCACCGCCGA+TGG | + | chr1.4:304204-304223 | MS.gene88977:CDS | 55.0% |
| CACCGCCGATGGGTGATATG+AGG | + | chr1.4:304215-304234 | MS.gene88977:CDS | 60.0% | |
| CGTGTCCACTCCACCAAAGG+TGG | + | chr1.4:304078-304097 | MS.gene88977:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 303749 | 304513 | 303749 | ID=MS.gene88977 |
| chr1.4 | mRNA | 303749 | 304513 | 303749 | ID=MS.gene88977.t1;Parent=MS.gene88977 |
| chr1.4 | exon | 303749 | 304513 | 303749 | ID=MS.gene88977.t1.exon1;Parent=MS.gene88977.t1 |
| chr1.4 | CDS | 303749 | 304513 | 303749 | ID=cds.MS.gene88977.t1;Parent=MS.gene88977.t1 |
| Gene Sequence |
| Protein sequence |
