Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91762.t1 | XP_013463539.1 | 94.2 | 208 | 12 | 0 | 1 | 208 | 3 | 210 | 5.70E-103 | 383.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91762.t1 | Q9ZRA4 | 73.1 | 208 | 55 | 1 | 1 | 207 | 1 | 208 | 4.4e-80 | 298.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91762.t1 | A0A072V680 | 94.2 | 208 | 12 | 0 | 1 | 208 | 3 | 210 | 4.1e-103 | 383.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene91762 | 0.836001 | 1.21E-56 | -1.69E-46 |
| MS.gene050139 | MS.gene91762 | 0.832113 | 1.14E-55 | -1.69E-46 |
| MS.gene050438 | MS.gene91762 | 0.817481 | 3.22E-52 | -1.69E-46 |
| MS.gene050584 | MS.gene91762 | 0.819204 | 1.31E-52 | -1.69E-46 |
| MS.gene050586 | MS.gene91762 | 0.832016 | 1.20E-55 | -1.69E-46 |
| MS.gene050780 | MS.gene91762 | 0.803642 | 3.16E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene91762 | 0.812778 | 3.57E-51 | -1.69E-46 |
| MS.gene051265 | MS.gene91762 | 0.802891 | 4.52E-49 | -1.69E-46 |
| MS.gene05143 | MS.gene91762 | 0.804159 | 2.47E-49 | -1.69E-46 |
| MS.gene051626 | MS.gene91762 | 0.834221 | 3.40E-56 | -1.69E-46 |
| MS.gene051789 | MS.gene91762 | 0.861581 | 9.28E-64 | -1.69E-46 |
| MS.gene052142 | MS.gene91762 | 0.853496 | 2.30E-61 | -1.69E-46 |
| MS.gene052187 | MS.gene91762 | 0.826117 | 3.23E-54 | -1.69E-46 |
| MS.gene052698 | MS.gene91762 | 0.847461 | 1.14E-59 | -1.69E-46 |
| MS.gene053074 | MS.gene91762 | 0.888492 | 5.63E-73 | -1.69E-46 |
| MS.gene053184 | MS.gene91762 | 0.802992 | 4.31E-49 | -1.69E-46 |
| MS.gene053383 | MS.gene91762 | 0.823461 | 1.37E-53 | -1.69E-46 |
| MS.gene053630 | MS.gene91762 | 0.805387 | 1.37E-49 | -1.69E-46 |
| MS.gene054184 | MS.gene91762 | 0.81034 | 1.21E-50 | -1.69E-46 |
| MS.gene054185 | MS.gene91762 | 0.816797 | 4.59E-52 | -1.69E-46 |
| MS.gene05456 | MS.gene91762 | 0.857032 | 2.15E-62 | -1.69E-46 |
| MS.gene054596 | MS.gene91762 | 0.819618 | 1.06E-52 | -1.69E-46 |
| MS.gene055359 | MS.gene91762 | 0.893678 | 5.02E-75 | -1.69E-46 |
| MS.gene058168 | MS.gene91762 | 0.891123 | 5.29E-74 | -1.69E-46 |
| MS.gene059006 | MS.gene91762 | 0.880973 | 3.53E-70 | -1.69E-46 |
| MS.gene059124 | MS.gene91762 | 0.848107 | 7.56E-60 | -1.69E-46 |
| MS.gene059632 | MS.gene91762 | 0.814138 | 1.79E-51 | -1.69E-46 |
| MS.gene060320 | MS.gene91762 | 0.815989 | 6.97E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91762.t1 | MTR_2g041570 | 94.231 | 208 | 12 | 0 | 1 | 208 | 3 | 210 | 2.98e-141 | 393 |
| MS.gene91762.t1 | MTR_2g041580 | 92.788 | 208 | 15 | 0 | 1 | 208 | 3 | 210 | 1.46e-139 | 389 |
| MS.gene91762.t1 | MTR_2g041550 | 84.135 | 208 | 33 | 0 | 1 | 208 | 3 | 210 | 4.04e-126 | 354 |
| MS.gene91762.t1 | MTR_8g020590 | 65.385 | 208 | 72 | 0 | 1 | 208 | 1 | 208 | 1.50e-94 | 275 |
| MS.gene91762.t1 | MTR_8g020630 | 55.502 | 209 | 89 | 3 | 2 | 208 | 3 | 209 | 7.64e-79 | 235 |
| MS.gene91762.t1 | MTR_8g020650 | 56.995 | 193 | 81 | 2 | 16 | 208 | 19 | 209 | 6.05e-77 | 230 |
| MS.gene91762.t1 | MTR_8g020670 | 54.545 | 209 | 91 | 3 | 2 | 208 | 3 | 209 | 3.96e-76 | 228 |
| MS.gene91762.t1 | MTR_8g020610 | 52.404 | 208 | 93 | 2 | 5 | 208 | 2 | 207 | 6.37e-74 | 222 |
| MS.gene91762.t1 | MTR_2g044040 | 49.282 | 209 | 102 | 3 | 2 | 208 | 3 | 209 | 9.90e-69 | 209 |
| MS.gene91762.t1 | MTR_2g045100 | 49.761 | 209 | 101 | 3 | 2 | 208 | 3 | 209 | 3.75e-67 | 205 |
| MS.gene91762.t1 | MTR_2g036120 | 50.000 | 208 | 100 | 3 | 2 | 207 | 3 | 208 | 8.03e-66 | 202 |
| MS.gene91762.t1 | MTR_3g019710 | 48.325 | 209 | 104 | 3 | 2 | 208 | 3 | 209 | 6.24e-65 | 199 |
| MS.gene91762.t1 | MTR_3g019650 | 48.077 | 208 | 105 | 3 | 2 | 208 | 3 | 208 | 1.03e-63 | 196 |
| MS.gene91762.t1 | MTR_3g019680 | 48.077 | 208 | 105 | 3 | 2 | 208 | 3 | 208 | 3.00e-63 | 195 |
| MS.gene91762.t1 | MTR_4g010340 | 48.077 | 208 | 105 | 3 | 1 | 208 | 1 | 205 | 1.62e-60 | 188 |
| MS.gene91762.t1 | MTR_0284s0010 | 45.933 | 209 | 109 | 3 | 2 | 208 | 3 | 209 | 1.39e-59 | 186 |
| MS.gene91762.t1 | MTR_5g031460 | 44.828 | 203 | 110 | 2 | 6 | 208 | 9 | 209 | 3.94e-58 | 182 |
| MS.gene91762.t1 | MTR_8g020770 | 44.076 | 211 | 114 | 3 | 1 | 208 | 3 | 212 | 2.52e-57 | 180 |
| MS.gene91762.t1 | MTR_7g011340 | 45.876 | 194 | 103 | 2 | 15 | 208 | 18 | 209 | 1.35e-56 | 178 |
| MS.gene91762.t1 | MTR_8g020750 | 44.550 | 211 | 113 | 3 | 1 | 208 | 3 | 212 | 1.82e-56 | 178 |
| MS.gene91762.t1 | MTR_3g073170 | 44.444 | 189 | 99 | 4 | 21 | 208 | 27 | 210 | 1.63e-55 | 176 |
| MS.gene91762.t1 | MTR_8g020760 | 44.231 | 208 | 112 | 3 | 3 | 208 | 6 | 211 | 3.48e-55 | 175 |
| MS.gene91762.t1 | MTR_2g019780 | 42.453 | 212 | 111 | 5 | 3 | 208 | 6 | 212 | 1.65e-52 | 168 |
| MS.gene91762.t1 | MTR_3g019810 | 44.390 | 205 | 104 | 4 | 2 | 204 | 109 | 305 | 7.99e-51 | 167 |
| MS.gene91762.t1 | MTR_8g020763 | 40.782 | 179 | 105 | 1 | 30 | 208 | 13 | 190 | 1.13e-47 | 155 |
| MS.gene91762.t1 | MTR_2g030855 | 42.328 | 189 | 102 | 3 | 18 | 202 | 25 | 210 | 2.59e-44 | 147 |
| MS.gene91762.t1 | MTR_2g031270 | 41.667 | 192 | 105 | 3 | 15 | 202 | 23 | 211 | 1.58e-43 | 145 |
| MS.gene91762.t1 | MTR_1g079490 | 40.566 | 212 | 110 | 5 | 6 | 202 | 5 | 215 | 1.62e-40 | 137 |
| MS.gene91762.t1 | MTR_4g017050 | 39.437 | 213 | 112 | 5 | 5 | 202 | 4 | 214 | 7.72e-40 | 136 |
| MS.gene91762.t1 | MTR_5g046410 | 39.437 | 213 | 112 | 5 | 5 | 202 | 4 | 214 | 8.24e-40 | 135 |
| MS.gene91762.t1 | MTR_5g046430 | 39.437 | 213 | 112 | 5 | 5 | 202 | 4 | 214 | 8.24e-40 | 135 |
| MS.gene91762.t1 | MTR_4g017030 | 39.437 | 213 | 112 | 5 | 5 | 202 | 4 | 214 | 8.24e-40 | 135 |
| MS.gene91762.t1 | MTR_1g023770 | 42.012 | 169 | 94 | 2 | 38 | 202 | 39 | 207 | 1.63e-39 | 134 |
| MS.gene91762.t1 | MTR_6g005310 | 39.070 | 215 | 116 | 5 | 1 | 202 | 1 | 213 | 4.08e-39 | 134 |
| MS.gene91762.t1 | MTR_6g005360 | 39.070 | 215 | 116 | 5 | 1 | 202 | 1 | 213 | 6.15e-39 | 134 |
| MS.gene91762.t1 | MTR_1g060120 | 41.317 | 167 | 97 | 1 | 38 | 204 | 56 | 221 | 6.96e-39 | 134 |
| MS.gene91762.t1 | MTR_2g030895 | 41.765 | 170 | 94 | 3 | 38 | 202 | 7 | 176 | 1.07e-38 | 132 |
| MS.gene91762.t1 | MTR_1g112200 | 36.139 | 202 | 124 | 2 | 1 | 202 | 9 | 205 | 2.24e-38 | 132 |
| MS.gene91762.t1 | MTR_2g030865 | 41.765 | 170 | 94 | 3 | 38 | 202 | 7 | 176 | 2.50e-38 | 130 |
| MS.gene91762.t1 | MTR_6g005340 | 38.605 | 215 | 117 | 5 | 1 | 202 | 1 | 213 | 3.03e-38 | 132 |
| MS.gene91762.t1 | MTR_1g085140 | 39.785 | 186 | 110 | 1 | 21 | 204 | 30 | 215 | 4.40e-38 | 131 |
| MS.gene91762.t1 | MTR_2g019250 | 38.028 | 213 | 115 | 5 | 5 | 202 | 4 | 214 | 4.54e-38 | 131 |
| MS.gene91762.t1 | MTR_7g111240 | 40.860 | 186 | 105 | 2 | 21 | 204 | 26 | 208 | 5.00e-38 | 131 |
| MS.gene91762.t1 | MTR_4g017040 | 38.028 | 213 | 115 | 5 | 5 | 202 | 4 | 214 | 8.21e-38 | 130 |
| MS.gene91762.t1 | MTR_7g111240 | 40.323 | 186 | 109 | 1 | 21 | 204 | 26 | 211 | 1.49e-37 | 130 |
| MS.gene91762.t1 | MTR_6g005330 | 40.957 | 188 | 103 | 4 | 21 | 202 | 28 | 213 | 1.08e-36 | 128 |
| MS.gene91762.t1 | MTR_2g072560 | 53.571 | 112 | 50 | 2 | 2 | 113 | 6 | 115 | 2.56e-35 | 122 |
| MS.gene91762.t1 | MTR_4g027415 | 48.031 | 127 | 64 | 2 | 15 | 141 | 18 | 142 | 3.43e-34 | 119 |
| MS.gene91762.t1 | MTR_5g036570 | 35.450 | 189 | 119 | 2 | 21 | 206 | 33 | 221 | 6.35e-34 | 120 |
| MS.gene91762.t1 | MTR_1g085500 | 35.326 | 184 | 117 | 1 | 21 | 202 | 29 | 212 | 1.76e-33 | 119 |
| MS.gene91762.t1 | MTR_2g031300 | 52.830 | 106 | 48 | 1 | 38 | 141 | 7 | 112 | 1.02e-31 | 113 |
| MS.gene91762.t1 | MTR_6g005380 | 40.789 | 152 | 83 | 3 | 21 | 167 | 28 | 177 | 2.61e-29 | 108 |
| MS.gene91762.t1 | MTR_3g019610 | 51.456 | 103 | 48 | 2 | 2 | 104 | 6 | 106 | 3.04e-29 | 105 |
| MS.gene91762.t1 | MTR_8g007300 | 35.593 | 177 | 101 | 5 | 2 | 167 | 5 | 179 | 8.40e-29 | 107 |
| MS.gene91762.t1 | MTR_6g005350 | 35.829 | 187 | 113 | 4 | 21 | 202 | 28 | 212 | 1.92e-28 | 106 |
| MS.gene91762.t1 | MTR_3g096330 | 36.022 | 186 | 92 | 5 | 7 | 173 | 559 | 736 | 2.94e-27 | 108 |
| MS.gene91762.t1 | MTR_2g086630 | 32.620 | 187 | 103 | 4 | 23 | 201 | 30 | 201 | 2.26e-24 | 95.9 |
| MS.gene91762.t1 | MTR_2g086620 | 31.100 | 209 | 120 | 5 | 1 | 201 | 9 | 201 | 4.69e-24 | 94.7 |
| MS.gene91762.t1 | MTR_2g086640 | 32.683 | 205 | 122 | 5 | 1 | 201 | 8 | 200 | 7.46e-24 | 94.4 |
| MS.gene91762.t1 | MTR_4g052780 | 34.109 | 129 | 84 | 1 | 74 | 201 | 74 | 202 | 2.07e-18 | 80.1 |
| MS.gene91762.t1 | MTR_4g052770 | 34.109 | 129 | 84 | 1 | 74 | 201 | 74 | 202 | 2.66e-18 | 79.7 |
| MS.gene91762.t1 | MTR_2g096110 | 29.134 | 127 | 74 | 2 | 41 | 166 | 6 | 117 | 7.28e-15 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91762.t1 | AT5G20630 | 64.904 | 208 | 72 | 1 | 1 | 207 | 3 | 210 | 7.08e-94 | 273 |
| MS.gene91762.t1 | AT1G72610 | 64.423 | 208 | 74 | 0 | 1 | 208 | 1 | 208 | 4.36e-89 | 261 |
| MS.gene91762.t1 | AT5G39160 | 42.632 | 190 | 101 | 3 | 21 | 205 | 30 | 216 | 2.54e-42 | 142 |
| MS.gene91762.t1 | AT3G04200 | 44.503 | 191 | 100 | 3 | 21 | 205 | 33 | 223 | 3.80e-42 | 142 |
| MS.gene91762.t1 | AT1G18970 | 41.176 | 204 | 114 | 3 | 3 | 202 | 15 | 216 | 7.11e-42 | 141 |
| MS.gene91762.t1 | AT1G18980 | 40.686 | 204 | 116 | 3 | 1 | 202 | 16 | 216 | 7.67e-42 | 141 |
| MS.gene91762.t1 | AT5G39190 | 42.932 | 191 | 103 | 3 | 21 | 205 | 30 | 220 | 2.21e-41 | 140 |
| MS.gene91762.t1 | AT5G39160 | 42.932 | 191 | 103 | 3 | 21 | 205 | 30 | 220 | 2.41e-41 | 140 |
| MS.gene91762.t1 | AT5G39130 | 42.408 | 191 | 104 | 3 | 21 | 205 | 30 | 220 | 5.85e-41 | 139 |
| MS.gene91762.t1 | AT3G62020 | 36.408 | 206 | 121 | 3 | 7 | 202 | 8 | 213 | 2.60e-39 | 134 |
| MS.gene91762.t1 | AT5G39110 | 41.379 | 203 | 108 | 5 | 11 | 202 | 16 | 218 | 7.10e-39 | 133 |
| MS.gene91762.t1 | AT3G05950 | 42.857 | 189 | 100 | 4 | 21 | 202 | 32 | 219 | 2.28e-38 | 132 |
| MS.gene91762.t1 | AT5G39120 | 39.906 | 213 | 117 | 5 | 1 | 202 | 5 | 217 | 3.55e-38 | 132 |
| MS.gene91762.t1 | AT1G02335 | 34.634 | 205 | 132 | 1 | 3 | 205 | 12 | 216 | 9.07e-38 | 130 |
| MS.gene91762.t1 | AT5G38960 | 41.327 | 196 | 100 | 6 | 21 | 205 | 32 | 223 | 1.64e-37 | 130 |
| MS.gene91762.t1 | AT5G39150 | 39.437 | 213 | 118 | 5 | 1 | 202 | 5 | 217 | 2.96e-37 | 129 |
| MS.gene91762.t1 | AT3G04150 | 40.000 | 195 | 109 | 4 | 21 | 208 | 32 | 225 | 3.74e-37 | 129 |
| MS.gene91762.t1 | AT5G39180 | 39.437 | 213 | 118 | 5 | 1 | 202 | 5 | 217 | 4.81e-37 | 129 |
| MS.gene91762.t1 | AT1G09560 | 42.391 | 184 | 104 | 2 | 21 | 202 | 30 | 213 | 1.85e-36 | 127 |
| MS.gene91762.t1 | AT3G04150 | 40.223 | 179 | 102 | 2 | 35 | 208 | 60 | 238 | 2.15e-36 | 127 |
| MS.gene91762.t1 | AT5G26700 | 36.634 | 202 | 122 | 3 | 1 | 201 | 10 | 206 | 2.61e-36 | 127 |
| MS.gene91762.t1 | AT3G62020 | 37.427 | 171 | 104 | 2 | 35 | 202 | 14 | 184 | 5.48e-36 | 125 |
| MS.gene91762.t1 | AT5G61750 | 43.609 | 133 | 75 | 0 | 35 | 167 | 41 | 173 | 6.02e-36 | 125 |
| MS.gene91762.t1 | AT5G38940 | 45.033 | 151 | 79 | 2 | 21 | 167 | 32 | 182 | 1.16e-35 | 125 |
| MS.gene91762.t1 | AT3G04190 | 42.683 | 164 | 88 | 3 | 21 | 179 | 32 | 194 | 1.41e-35 | 125 |
| MS.gene91762.t1 | AT5G38940 | 43.871 | 155 | 83 | 2 | 17 | 167 | 52 | 206 | 2.08e-35 | 125 |
| MS.gene91762.t1 | AT3G04170 | 41.885 | 191 | 100 | 6 | 21 | 204 | 32 | 218 | 4.56e-35 | 124 |
| MS.gene91762.t1 | AT3G10080 | 37.037 | 189 | 113 | 2 | 19 | 202 | 30 | 217 | 7.88e-35 | 123 |
| MS.gene91762.t1 | AT5G38930 | 45.033 | 151 | 79 | 2 | 21 | 167 | 32 | 182 | 1.00e-34 | 123 |
| MS.gene91762.t1 | AT5G39190 | 42.331 | 163 | 89 | 2 | 48 | 205 | 36 | 198 | 1.46e-34 | 122 |
| MS.gene91762.t1 | AT5G39160 | 42.331 | 163 | 89 | 2 | 48 | 205 | 36 | 198 | 1.56e-34 | 122 |
| MS.gene91762.t1 | AT3G04180 | 40.104 | 192 | 105 | 6 | 21 | 204 | 32 | 221 | 2.21e-34 | 122 |
| MS.gene91762.t1 | AT3G10080 | 37.037 | 189 | 113 | 2 | 19 | 202 | 101 | 288 | 3.15e-34 | 124 |
| MS.gene91762.t1 | AT5G38910 | 39.720 | 214 | 111 | 7 | 2 | 202 | 9 | 217 | 1.59e-33 | 120 |
| MS.gene91762.t1 | AT3G05930 | 37.019 | 208 | 123 | 4 | 2 | 201 | 5 | 212 | 1.77e-33 | 119 |
| MS.gene91762.t1 | AT1G74820 | 36.224 | 196 | 121 | 2 | 9 | 202 | 30 | 223 | 3.05e-33 | 119 |
| MS.gene91762.t1 | AT4G14630 | 38.095 | 189 | 110 | 3 | 21 | 202 | 32 | 220 | 9.22e-33 | 117 |
| MS.gene91762.t1 | AT1G10460 | 36.932 | 176 | 107 | 3 | 35 | 206 | 42 | 217 | 5.53e-31 | 113 |
| MS.gene91762.t1 | AT5G38910 | 31.132 | 212 | 95 | 7 | 2 | 202 | 9 | 180 | 1.06e-14 | 69.7 |
Find 49 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGAACGACCAACATTAATT+TGG | 0.178858 | 2.1:+49619817 | None:intergenic |
| GAAGTATTGGTTGTTATTAA+AGG | 0.278056 | 2.1:-49619939 | MS.gene91762:CDS |
| AAGAAGCTTAAGAGTGTTCT+TGG | 0.300035 | 2.1:-49619666 | MS.gene91762:CDS |
| ATAATGTTTGTGGTGTTTCC+AGG | 0.357728 | 2.1:+49620089 | None:intergenic |
| GAATATCCGGCAGGGCCATT+TGG | 0.383798 | 2.1:+49620170 | None:intergenic |
| TAACCCTCGTCTACAGATAT+TGG | 0.423623 | 2.1:-49619763 | MS.gene91762:CDS |
| GCACAATTTCCCGGTGTAAA+CGG | 0.428355 | 2.1:-49620038 | MS.gene91762:CDS |
| CCCCGGTGCTTCGGAAGTAT+TGG | 0.434736 | 2.1:-49619952 | MS.gene91762:CDS |
| TTTCCCGGTGTAAACGGTCT+TGG | 0.446321 | 2.1:-49620032 | MS.gene91762:CDS |
| AAGAGTGTTCTTGGAGGTAG+TGG | 0.449452 | 2.1:-49619657 | MS.gene91762:CDS |
| TGGTGGTTTGCAAGAATATC+CGG | 0.462858 | 2.1:+49620157 | None:intergenic |
| AACCAATACTTCCGAAGCAC+CGG | 0.468211 | 2.1:+49619950 | None:intergenic |
| TCACACACACCCCGGTGCTT+CGG | 0.471434 | 2.1:-49619961 | MS.gene91762:CDS |
| TGGTGTTTCCAGGTGCGGCT+AGG | 0.473117 | 2.1:+49620099 | None:intergenic |
| TTCCAGGTGCGGCTAGGCCG+TGG | 0.474288 | 2.1:+49620105 | None:intergenic |
| GCATTCGACGCACAATTTCC+CGG | 0.479197 | 2.1:-49620047 | MS.gene91762:CDS |
| TACCACGGCCTAGCCGCACC+TGG | 0.482390 | 2.1:-49620107 | MS.gene91762:CDS |
| GGCTATATCTAAACGCGCCA+TGG | 0.483268 | 2.1:+49620004 | None:intergenic |
| AGCGGCTTTGATAATGTTTG+TGG | 0.483382 | 2.1:+49620079 | None:intergenic |
| GGAAATTGTGCGTCGAATGC+AGG | 0.492256 | 2.1:+49620050 | None:intergenic |
| ATTAAAGGAACAATCAGTGC+TGG | 0.495019 | 2.1:-49619924 | MS.gene91762:CDS |
| AATGCACCAAATGGCCCTGC+CGG | 0.514872 | 2.1:-49620176 | MS.gene91762:CDS |
| TTGCATTTCCAAATTAATGT+TGG | 0.515849 | 2.1:-49619825 | MS.gene91762:CDS |
| ACCAATACTTCCGAAGCACC+GGG | 0.531532 | 2.1:+49619951 | None:intergenic |
| GCAGATTACAATGCACCAAA+TGG | 0.532463 | 2.1:-49620185 | MS.gene91762:CDS |
| CGTTTAGATATAGCCGTTGG+TGG | 0.560060 | 2.1:-49619996 | MS.gene91762:CDS |
| AAGCTTAAGAGTGTTCTTGG+AGG | 0.564526 | 2.1:-49619663 | MS.gene91762:CDS |
| AACGTAAACAACATTGTCAG+AGG | 0.574065 | 2.1:+49619893 | None:intergenic |
| GTTGCAGTAATAAACTCCGT+TGG | 0.592047 | 2.1:+49619714 | None:intergenic |
| GGTTTGCAAGAATATCCGGC+AGG | 0.596087 | 2.1:+49620161 | None:intergenic |
| ATTCCAAGACCGTTTACACC+GGG | 0.600502 | 2.1:+49620029 | None:intergenic |
| TGGAAATGCAACAATCCTTG+TGG | 0.601071 | 2.1:+49619837 | None:intergenic |
| GTTTGTGGTGTTTCCAGGTG+CGG | 0.608586 | 2.1:+49620094 | None:intergenic |
| TACGTTAAAACACTTCACAA+AGG | 0.611341 | 2.1:-49619876 | MS.gene91762:CDS |
| TGCTACGCAGAAATCTACTA+CGG | 0.612239 | 2.1:+49620205 | None:intergenic |
| GATTCCAAGACCGTTTACAC+CGG | 0.616073 | 2.1:+49620028 | None:intergenic |
| GCACCGGGGTGTGTGTGAAG+TGG | 0.618194 | 2.1:+49619966 | None:intergenic |
| GTGGTAGACGAAGTCATTCG+CGG | 0.625632 | 2.1:+49620124 | None:intergenic |
| AAACGGTCTTGGAATCTCCA+TGG | 0.625728 | 2.1:-49620021 | MS.gene91762:CDS |
| AAGTGGAATAACACCACCAA+CGG | 0.630116 | 2.1:+49619983 | None:intergenic |
| ATTCCACTTCACACACACCC+CGG | 0.641125 | 2.1:-49619969 | MS.gene91762:CDS |
| GCGCGTTTAGATATAGCCGT+TGG | 0.647288 | 2.1:-49619999 | MS.gene91762:CDS |
| TAATCCAATATCTGTAGACG+AGG | 0.649322 | 2.1:+49619759 | None:intergenic |
| GTTTGCAAGAATATCCGGCA+GGG | 0.671962 | 2.1:+49620162 | None:intergenic |
| GTCGAATGCAGGTGCCACAG+CGG | 0.697381 | 2.1:+49620061 | None:intergenic |
| GCGAATGACTTCGTCTACCA+CGG | 0.705558 | 2.1:-49620122 | MS.gene91762:CDS |
| AAACATTATCAAAGCCGCTG+TGG | 0.716343 | 2.1:-49620075 | MS.gene91762:CDS |
| AATCCAATATCTGTAGACGA+GGG | 0.723455 | 2.1:+49619760 | None:intergenic |
| CCAATACTTCCGAAGCACCG+GGG | 0.761220 | 2.1:+49619952 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAGAAAAAATGAAGAAGA+TGG | + | chr2.1:49619666-49619685 | None:intergenic | 25.0% |
| ! | TTGCATTTCCAAATTAATGT+TGG | - | chr2.1:49620085-49620104 | MS.gene91762:CDS | 25.0% |
| !!! | GAAGTATTGGTTGTTATTAA+AGG | - | chr2.1:49619971-49619990 | MS.gene91762:CDS | 25.0% |
| TACGTTAAAACACTTCACAA+AGG | - | chr2.1:49620034-49620053 | MS.gene91762:CDS | 30.0% | |
| TAGAACGACCAACATTAATT+TGG | + | chr2.1:49620096-49620115 | None:intergenic | 30.0% | |
| ! | GATGTTATGATTTTTCCACA+AGG | - | chr2.1:49620058-49620077 | MS.gene91762:CDS | 30.0% |
| AACGTAAACAACATTGTCAG+AGG | + | chr2.1:49620020-49620039 | None:intergenic | 35.0% | |
| AATCCAATATCTGTAGACGA+GGG | + | chr2.1:49620153-49620172 | None:intergenic | 35.0% | |
| ATAATGTTTGTGGTGTTTCC+AGG | + | chr2.1:49619824-49619843 | None:intergenic | 35.0% | |
| ATTAAAGGAACAATCAGTGC+TGG | - | chr2.1:49619986-49620005 | MS.gene91762:CDS | 35.0% | |
| TAATCCAATATCTGTAGACG+AGG | + | chr2.1:49620154-49620173 | None:intergenic | 35.0% | |
| ! | GTTTGAAAGCGATTTTCCAA+CGG | - | chr2.1:49620180-49620199 | MS.gene91762:CDS | 35.0% |
| !! | AAGAAGCTTAAGAGTGTTCT+TGG | - | chr2.1:49620244-49620263 | MS.gene91762:CDS | 35.0% |
| AAACATTATCAAAGCCGCTG+TGG | - | chr2.1:49619835-49619854 | MS.gene91762:CDS | 40.0% | |
| AAGTGGAATAACACCACCAA+CGG | + | chr2.1:49619930-49619949 | None:intergenic | 40.0% | |
| GCAGATTACAATGCACCAAA+TGG | - | chr2.1:49619725-49619744 | MS.gene91762:CDS | 40.0% | |
| GTTGCAGTAATAAACTCCGT+TGG | + | chr2.1:49620199-49620218 | None:intergenic | 40.0% | |
| TAACCCTCGTCTACAGATAT+TGG | - | chr2.1:49620147-49620166 | MS.gene91762:CDS | 40.0% | |
| TGCTACGCAGAAATCTACTA+CGG | + | chr2.1:49619708-49619727 | None:intergenic | 40.0% | |
| TGGAAATGCAACAATCCTTG+TGG | + | chr2.1:49620076-49620095 | None:intergenic | 40.0% | |
| ! | TGGTGGTTTGCAAGAATATC+CGG | + | chr2.1:49619756-49619775 | None:intergenic | 40.0% |
| !! | AAGCTTAAGAGTGTTCTTGG+AGG | - | chr2.1:49620247-49620266 | MS.gene91762:CDS | 40.0% |
| !! | AGCGGCTTTGATAATGTTTG+TGG | + | chr2.1:49619834-49619853 | None:intergenic | 40.0% |
| !!! | TCATTCGCGGTTACTTTTGA+TGG | + | chr2.1:49619776-49619795 | None:intergenic | 40.0% |
| AAACGGTCTTGGAATCTCCA+TGG | - | chr2.1:49619889-49619908 | MS.gene91762:CDS | 45.0% | |
| AACCAATACTTCCGAAGCAC+CGG | + | chr2.1:49619963-49619982 | None:intergenic | 45.0% | |
| ATTCCAAGACCGTTTACACC+GGG | + | chr2.1:49619884-49619903 | None:intergenic | 45.0% | |
| CGTTTAGATATAGCCGTTGG+TGG | - | chr2.1:49619914-49619933 | MS.gene91762:CDS | 45.0% | |
| GATTCCAAGACCGTTTACAC+CGG | + | chr2.1:49619885-49619904 | None:intergenic | 45.0% | |
| GCACAATTTCCCGGTGTAAA+CGG | - | chr2.1:49619872-49619891 | MS.gene91762:CDS | 45.0% | |
| GTTTGCAAGAATATCCGGCA+GGG | + | chr2.1:49619751-49619770 | None:intergenic | 45.0% | |
| !! | AAGAGTGTTCTTGGAGGTAG+TGG | - | chr2.1:49620253-49620272 | MS.gene91762:CDS | 45.0% |
| !!! | TTCGCGGTTACTTTTGATGG+TGG | + | chr2.1:49619773-49619792 | None:intergenic | 45.0% |
| ATTCCACTTCACACACACCC+CGG | - | chr2.1:49619941-49619960 | MS.gene91762:CDS | 50.0% | |
| GCATTCGACGCACAATTTCC+CGG | - | chr2.1:49619863-49619882 | MS.gene91762:CDS | 50.0% | |
| GCGAATGACTTCGTCTACCA+CGG | - | chr2.1:49619788-49619807 | MS.gene91762:CDS | 50.0% | |
| GCGCGTTTAGATATAGCCGT+TGG | - | chr2.1:49619911-49619930 | MS.gene91762:CDS | 50.0% | |
| GGAAATTGTGCGTCGAATGC+AGG | + | chr2.1:49619863-49619882 | None:intergenic | 50.0% | |
| GGCTATATCTAAACGCGCCA+TGG | + | chr2.1:49619909-49619928 | None:intergenic | 50.0% | |
| GGTTTGCAAGAATATCCGGC+AGG | + | chr2.1:49619752-49619771 | None:intergenic | 50.0% | |
| GTGGTAGACGAAGTCATTCG+CGG | + | chr2.1:49619789-49619808 | None:intergenic | 50.0% | |
| GTTTGTGGTGTTTCCAGGTG+CGG | + | chr2.1:49619819-49619838 | None:intergenic | 50.0% | |
| TTTCCCGGTGTAAACGGTCT+TGG | - | chr2.1:49619878-49619897 | MS.gene91762:CDS | 50.0% | |
| ! | ACCAATACTTCCGAAGCACC+GGG | + | chr2.1:49619962-49619981 | None:intergenic | 50.0% |
| AATGCACCAAATGGCCCTGC+CGG | - | chr2.1:49619734-49619753 | MS.gene91762:CDS | 55.0% | |
| GAATATCCGGCAGGGCCATT+TGG | + | chr2.1:49619743-49619762 | None:intergenic | 55.0% | |
| !! | CCAATACTTCCGAAGCACCG+GGG | + | chr2.1:49619961-49619980 | None:intergenic | 55.0% |
| TCACACACACCCCGGTGCTT+CGG | - | chr2.1:49619949-49619968 | MS.gene91762:CDS | 60.0% | |
| TGGTGTTTCCAGGTGCGGCT+AGG | + | chr2.1:49619814-49619833 | None:intergenic | 60.0% | |
| ! | CCCCGGTGCTTCGGAAGTAT+TGG | - | chr2.1:49619958-49619977 | MS.gene91762:CDS | 60.0% |
| ! | GTCGAATGCAGGTGCCACAG+CGG | + | chr2.1:49619852-49619871 | None:intergenic | 60.0% |
| GCACCGGGGTGTGTGTGAAG+TGG | + | chr2.1:49619947-49619966 | None:intergenic | 65.0% | |
| TACCACGGCCTAGCCGCACC+TGG | - | chr2.1:49619803-49619822 | MS.gene91762:CDS | 70.0% | |
| TTCCAGGTGCGGCTAGGCCG+TGG | + | chr2.1:49619808-49619827 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 49619653 | 49620279 | 49619653 | ID=MS.gene91762 |
| chr2.1 | mRNA | 49619653 | 49620279 | 49619653 | ID=MS.gene91762.t1;Parent=MS.gene91762 |
| chr2.1 | exon | 49619653 | 49620279 | 49619653 | ID=MS.gene91762.t1.exon1;Parent=MS.gene91762.t1 |
| chr2.1 | CDS | 49619653 | 49620279 | 49619653 | ID=cds.MS.gene91762.t1;Parent=MS.gene91762.t1 |
| Gene Sequence |
| Protein sequence |
