AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene94781


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94781.t1 MTR_7g066240 100.000 51 0 0 1 51 1 51 9.17e-33 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94781.t1 AT5G41010 88.235 51 6 0 1 51 1 51 1.24e-29 98.6
MS.gene94781.t1 AT1G53690 73.171 41 10 1 2 41 10 50 3.67e-15 62.4
MS.gene94781.t1 AT1G53690 73.171 41 10 1 2 41 10 50 3.67e-15 62.4

Find 12 sgRNAs with CRISPR-Local

Find 11 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTAACGTGCTTCATAC+TGG 0.389614 7.1:-36252977 None:intergenic
AATAACGTCGCCAGACTTCA+AGG 0.419094 7.1:-36252898 None:intergenic
GACGAGTGCGCTTCTTGTAA+AGG 0.448256 7.1:-36252947 None:intergenic
GTCGAGAATACCTTGAAGTC+TGG 0.470760 7.1:+36252888 MS.gene94781:CDS
CAGAGGTAACTCACAGGCTC+AGG 0.525994 7.1:-36252852 None:intergenic
AGTTACCTCTGTGGTGATTG+TGG 0.528859 7.1:+36252864 MS.gene94781:CDS
TAACTCACAGGCTCAGGCAG+AGG 0.539104 7.1:-36252846 None:intergenic
GTTATTCAGTGCCGTGACTG+TGG 0.582901 7.1:+36252915 MS.gene94781:CDS
GGATTCTGTAACCACAGTCA+CGG 0.652403 7.1:-36252926 None:intergenic
TCACCACAGAGGTAACTCAC+AGG 0.674710 7.1:-36252858 None:intergenic
GAGCCTGTGAGTTACCTCTG+TGG 0.764675 7.1:+36252855 MS.gene94781:CDS
CGACTCCACAATCACCACAG+AGG 0.769265 7.1:-36252869 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AATAACGTCGCCAGACTTCA+AGG - chr7.1:36252901-36252920 None:intergenic 45.0%
AGTTACCTCTGTGGTGATTG+TGG + chr7.1:36252864-36252883 MS.gene94781:CDS 45.0%
GGATTCTGTAACCACAGTCA+CGG - chr7.1:36252929-36252948 None:intergenic 45.0%
GTCGAGAATACCTTGAAGTC+TGG + chr7.1:36252888-36252907 MS.gene94781:CDS 45.0%
GACGAGTGCGCTTCTTGTAA+AGG - chr7.1:36252950-36252969 None:intergenic 50.0%
GTTATTCAGTGCCGTGACTG+TGG + chr7.1:36252915-36252934 MS.gene94781:CDS 50.0%
TCACCACAGAGGTAACTCAC+AGG - chr7.1:36252861-36252880 None:intergenic 50.0%
CAGAGGTAACTCACAGGCTC+AGG - chr7.1:36252855-36252874 None:intergenic 55.0%
CGACTCCACAATCACCACAG+AGG - chr7.1:36252872-36252891 None:intergenic 55.0%
GAGCCTGTGAGTTACCTCTG+TGG + chr7.1:36252855-36252874 MS.gene94781:CDS 55.0%
TAACTCACAGGCTCAGGCAG+AGG - chr7.1:36252849-36252868 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 36252840 36252995 36252840 ID=MS.gene94781
chr7.1 mRNA 36252840 36252995 36252840 ID=MS.gene94781.t1;Parent=MS.gene94781
chr7.1 exon 36252840 36252995 36252840 ID=MS.gene94781.t1.exon1;Parent=MS.gene94781.t1
chr7.1 CDS 36252840 36252995 36252840 ID=cds.MS.gene94781.t1;Parent=MS.gene94781.t1
Gene Sequence

>MS.gene94781

ATGGATCCTCTGCCTGAGCCTGTGAGTTACCTCTGTGGTGATTGTGGAGTCGAGAATACCTTGAAGTCTGGCGACGTTATTCAGTGCCGTGACTGTGGTTACAGAATCCTTTACAAGAAGCGCACTCGTCGCATTGTCCAGTATGAAGCACGTTAA

Protein sequence

>MS.gene94781.t1

MDPLPEPVSYLCGDCGVENTLKSGDVIQCRDCGYRILYKKRTRRIVQYEAR