AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene97479


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97479.t1 MTR_7g056337 100.000 153 0 0 1 153 63 215 2.29e-112 317
MS.gene97479.t1 MTR_8g087430 71.242 153 44 0 1 153 63 215 6.33e-79 233
MS.gene97479.t1 MTR_4g075550 34.839 155 86 5 12 153 117 269 2.14e-22 90.1
MS.gene97479.t1 MTR_3g113920 34.783 161 90 5 6 153 111 269 9.68e-22 88.6
MS.gene97479.t1 MTR_8g012615 33.129 163 94 3 1 150 40 200 6.22e-19 79.7
MS.gene97479.t1 MTR_8g012595 31.579 152 88 3 12 149 118 267 3.51e-16 73.6
MS.gene97479.t1 MTR_8g012605 36.036 111 64 3 1 107 107 214 2.91e-14 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97479.t1 AT3G25040 85.621 153 22 0 1 153 63 215 9.09e-98 281
MS.gene97479.t1 AT1G29330 70.588 153 45 0 1 153 1 153 1.15e-79 233
MS.gene97479.t1 AT1G29330 70.588 153 45 0 1 153 63 215 1.34e-79 234
MS.gene97479.t1 AT1G75760 34.783 161 90 5 6 153 111 269 2.04e-22 90.1
MS.gene97479.t1 AT1G19970 35.484 155 85 5 12 153 117 269 5.52e-22 89.0
MS.gene97479.t1 AT4G38790 33.735 166 95 5 1 153 107 270 2.28e-21 87.4
MS.gene97479.t1 AT1G75760 33.784 148 84 4 6 141 111 256 1.07e-20 85.5
MS.gene97479.t1 AT3G25160 33.333 156 87 5 6 147 113 265 9.16e-20 83.2
MS.gene97479.t1 AT2G21190 31.928 166 98 5 1 153 103 266 1.45e-19 82.8
MS.gene97479.t1 AT1G75760 35.238 105 63 2 6 107 111 213 7.16e-17 74.7
MS.gene97479.t1 AT1G19970 36.364 99 58 2 12 107 117 213 4.28e-16 72.8

Find 34 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATGAGAAATTCACATTTA+AGG 0.174751 7.4:-48339208 MS.gene97479:CDS
TGTTATGAAGTTGATATTCT+TGG 0.275463 7.4:-48339358 None:intergenic
GGACAATATGTCTTTCTTCT+TGG 0.280895 7.4:-48338954 MS.gene97479:CDS
AGTATTGCCCTGCTTATTGT+TGG 0.324545 7.4:-48339241 MS.gene97479:CDS
AGAAACATTGACAATTTAAC+AGG 0.337581 7.4:-48338975 MS.gene97479:CDS
GACAATATGTCTTTCTTCTT+GGG 0.345288 7.4:-48338953 MS.gene97479:intron
GAGCACTATACATTCTCAAC+TGG 0.354642 7.4:-48338215 MS.gene97479:CDS
TGAATTTCTCATTGATAAGT+AGG 0.405613 7.4:+48339217 None:intergenic
CAGTACCAGTTGAGGAAGTA+TGG 0.407791 7.4:+48339010 None:intergenic
TGGCAACAGCTTCCAAATAC+AGG 0.408291 7.4:+48339030 None:intergenic
GTTATGAAGTTGATATTCTT+GGG 0.424245 7.4:-48339357 MS.gene97479:CDS
TGTTGCCATACTTCCTCAAC+TGG 0.450840 7.4:-48339015 MS.gene97479:CDS
CCGAGCCCCACTTTGTCCAT+TGG 0.453725 7.4:-48338176 MS.gene97479:CDS
ATGCTGGCAAATGAAGCTTC+TGG 0.490077 7.4:+48337851 None:intergenic
AAGTAGGGCCAACAATAAGC+AGG 0.512666 7.4:+48339233 None:intergenic
TAGATCATATGACAAAGATC+AGG 0.514146 7.4:-48339286 MS.gene97479:CDS
CCAATGGACAAAGTGGGGCT+CGG 0.529481 7.4:+48338176 None:intergenic
TACTTATCCAATGGACAAAG+TGG 0.529817 7.4:+48338169 None:intergenic
TATGGAGAATTACACTATGC+TGG 0.546192 7.4:+48337835 None:intergenic
CTTTGTAGCAGTACCAGTTG+AGG 0.551696 7.4:+48339002 None:intergenic
TTTCTATTGTATGGTACATG+AGG 0.554169 7.4:-48339326 MS.gene97479:CDS
TCTTCTACTACTATTTCCAA+AGG 0.567268 7.4:-48337975 MS.gene97479:intron
TGAGAAATTCACATTTAAGG+AGG 0.576216 7.4:-48339205 MS.gene97479:intron
GAATTTCTCATTGATAAGTA+GGG 0.579233 7.4:+48339218 None:intergenic
ACTTATCCAATGGACAAAGT+GGG 0.585109 7.4:+48338170 None:intergenic
TACTAGAAAATAGTGACGGA+AGG 0.599670 7.4:+48339260 None:intergenic
ACAGAATTATACTTATCCAA+TGG 0.602023 7.4:+48338160 None:intergenic
GGCAACAGCTTCCAAATACA+GGG 0.635959 7.4:+48339031 None:intergenic
CTTATCCAATGGACAAAGTG+GGG 0.636328 7.4:+48338171 None:intergenic
TATACATGGAATAGGTGATG+TGG 0.652075 7.4:-48339061 MS.gene97479:intron
AGCACTATACATTCTCAACT+GGG 0.652774 7.4:-48338214 MS.gene97479:CDS
GCAATACTAGAAAATAGTGA+CGG 0.652982 7.4:+48339256 None:intergenic
ATGATCTACGAACAATCTTG+TGG 0.654874 7.4:+48339301 None:intergenic
AGTAGGGCCAACAATAAGCA+GGG 0.670399 7.4:+48339234 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATTATTAACATAAATAAAA+TGG + chr7.4:48339276-48339295 None:intergenic 10.0%
!! ATGCAAATATATAATGTTAA+AGG + chr7.4:48338287-48338306 None:intergenic 15.0%
!! CTATAATAAAAAATAAGCAA+TGG - chr7.4:48338854-48338873 MS.gene97479:intron 15.0%
!! CTAGATAAATTATAGCTAAT+GGG - chr7.4:48338607-48338626 MS.gene97479:intron 20.0%
!! TAAAAACATTCAAACATGTA+TGG + chr7.4:48338069-48338088 None:intergenic 20.0%
!! TAGATAAATTATAGCTAATG+GGG - chr7.4:48338608-48338627 MS.gene97479:intron 20.0%
!!! AGTTGAAAGTTTTTATTTGT+TGG - chr7.4:48338741-48338760 MS.gene97479:intron 20.0%
!!! TCTTGTTTAATTGTTATCAT+TGG - chr7.4:48339067-48339086 MS.gene97479:intron 20.0%
! AAAGCTAAAACAAGTTCAAA+TGG + chr7.4:48338902-48338921 None:intergenic 25.0%
! AAGCTAAAACAAGTTCAAAT+GGG + chr7.4:48338901-48338920 None:intergenic 25.0%
! AATTAGCAGCTATTATATTC+TGG - chr7.4:48338339-48338358 MS.gene97479:intron 25.0%
! ACAGAATTATACTTATCCAA+TGG + chr7.4:48339046-48339065 None:intergenic 25.0%
! ACTGTAATGTTTAACAATGA+TGG + chr7.4:48339121-48339140 None:intergenic 25.0%
! AGAAACATTGACAATTTAAC+AGG - chr7.4:48338228-48338247 MS.gene97479:intron 25.0%
! ATATATTTGCATGATTTCTG+TGG - chr7.4:48338295-48338314 MS.gene97479:intron 25.0%
! ATCATTGTTAAACATTACAG+TGG - chr7.4:48339120-48339139 MS.gene97479:intron 25.0%
! CAATGAGAAATTCACATTTA+AGG - chr7.4:48337995-48338014 MS.gene97479:CDS 25.0%
! CCATTAGCTATAATTTATCT+AGG + chr7.4:48338609-48338628 None:intergenic 25.0%
! CCTAGATAAATTATAGCTAA+TGG - chr7.4:48338606-48338625 MS.gene97479:intron 25.0%
! GAATTTCTCATTGATAAGTA+GGG + chr7.4:48337988-48338007 None:intergenic 25.0%
! TACTATTTCCAAAGGTAAAT+TGG - chr7.4:48339236-48339255 MS.gene97479:CDS 25.0%
! TATAATTTATCTAGGTGAGT+TGG + chr7.4:48338601-48338620 None:intergenic 25.0%
! TGAATTTCTCATTGATAAGT+AGG + chr7.4:48337989-48338008 None:intergenic 25.0%
! TTTCATGTAACATGTTGTAT+AGG - chr7.4:48338559-48338578 MS.gene97479:intron 25.0%
!!! TTGAACTTGTTTTAGCTTTT+AGG - chr7.4:48338903-48338922 MS.gene97479:intron 25.0%
AAACTGCTTATACATGGAAT+AGG - chr7.4:48338134-48338153 MS.gene97479:intron 30.0%
AACCTTAATAAGCAGAACTT+TGG + chr7.4:48338799-48338818 None:intergenic 30.0%
ATGGTAACTTTGTATATCAG+AGG - chr7.4:48338873-48338892 MS.gene97479:intron 30.0%
CAACTCAAGATCATAATAGA+AGG + chr7.4:48338726-48338745 None:intergenic 30.0%
GACAATATGTCTTTCTTCTT+GGG - chr7.4:48338250-48338269 MS.gene97479:intron 30.0%
GCAATACTAGAAAATAGTGA+CGG + chr7.4:48337950-48337969 None:intergenic 30.0%
TAGATCATATGACAAAGATC+AGG - chr7.4:48337917-48337936 MS.gene97479:intron 30.0%
TCTTCTACTACTATTTCCAA+AGG - chr7.4:48339228-48339247 MS.gene97479:CDS 30.0%
TGAAAATATTGCCTTGTAGT+TGG - chr7.4:48339321-48339340 MS.gene97479:CDS 30.0%
TGAGAAATTCACATTTAAGG+AGG - chr7.4:48337998-48338017 MS.gene97479:CDS 30.0%
TTTCTATTGTATGGTACATG+AGG - chr7.4:48337877-48337896 MS.gene97479:intron 30.0%
! ATTCCATGTATAAGCAGTTT+TGG + chr7.4:48338134-48338153 None:intergenic 30.0%
! GAAGCTCATTTTCTATTGTA+TGG - chr7.4:48337868-48337887 MS.gene97479:intron 30.0%
! TTCCATGTATAAGCAGTTTT+GGG + chr7.4:48338133-48338152 None:intergenic 30.0%
! TTTATTTGTTGGAACGAAAC+TGG - chr7.4:48338752-48338771 MS.gene97479:intron 30.0%
ACTTATCCAATGGACAAAGT+GGG + chr7.4:48339036-48339055 None:intergenic 35.0%
AGCACTATACATTCTCAACT+GGG - chr7.4:48338989-48339008 MS.gene97479:CDS 35.0%
AGCCAAAGTTCTGCTTATTA+AGG - chr7.4:48338794-48338813 MS.gene97479:intron 35.0%
ATAAAATGGACCTGACAAGT+AGG + chr7.4:48339262-48339281 None:intergenic 35.0%
ATGATCTACGAACAATCTTG+TGG + chr7.4:48337905-48337924 None:intergenic 35.0%
GGACAATATGTCTTTCTTCT+TGG - chr7.4:48338249-48338268 MS.gene97479:intron 35.0%
GGCTTCAAGATCATAATAGA+AGG + chr7.4:48338778-48338797 None:intergenic 35.0%
TACTTATCCAATGGACAAAG+TGG + chr7.4:48339037-48339056 None:intergenic 35.0%
TATACATGGAATAGGTGATG+TGG - chr7.4:48338142-48338161 MS.gene97479:intron 35.0%
TTGAAACTGATCAATGAGAC+TGG - chr7.4:48338700-48338719 MS.gene97479:intron 35.0%
TTGGTTTATGCTCTTTAGAC+TGG - chr7.4:48339086-48339105 MS.gene97479:intron 35.0%
TTTCAATGAAGCCAACAAAC+AGG + chr7.4:48338686-48338705 None:intergenic 35.0%
! CATTACTTCTTTTGCAGCAT+GGG - chr7.4:48339169-48339188 MS.gene97479:intron 35.0%
! TACTAGAAAATAGTGACGGA+AGG + chr7.4:48337946-48337965 None:intergenic 35.0%
AATGGAAGGAAGTCAGAACA+AGG + chr7.4:48339153-48339172 None:intergenic 40.0%
AGACCAACCTTCCAGTTATT+TGG + chr7.4:48338390-48338409 None:intergenic 40.0%
AGTATTGCCCTGCTTATTGT+TGG - chr7.4:48337962-48337981 MS.gene97479:intron 40.0%
CAAGTAGGCCAATTTACCTT+TGG + chr7.4:48339247-48339266 None:intergenic 40.0%
CCATGCTGCAAAAGAAGTAA+TGG + chr7.4:48339171-48339190 None:intergenic 40.0%
CTGATCAACAGCCAAATAAC+TGG - chr7.4:48338376-48338395 MS.gene97479:intron 40.0%
CTTATCCAATGGACAAAGTG+GGG + chr7.4:48339035-48339054 None:intergenic 40.0%
CTTTCTTCTTGGGTAAGCTA+TGG - chr7.4:48338260-48338279 MS.gene97479:intron 40.0%
CTTTGTCTGTGAAATGCAGT+GGG - chr7.4:48338961-48338980 MS.gene97479:CDS 40.0%
GAGCACTATACATTCTCAAC+TGG - chr7.4:48338988-48339007 MS.gene97479:CDS 40.0%
GCTCAAAACTAAGACGAACA+AGG + chr7.4:48339298-48339317 None:intergenic 40.0%
GTCCCAAAACTGCTTATACA+TGG - chr7.4:48338128-48338147 MS.gene97479:intron 40.0%
TAGCTAATGGGGACAACAAA+AGG - chr7.4:48338619-48338638 MS.gene97479:intron 40.0%
TCAACAGCCAAATAACTGGA+AGG - chr7.4:48338380-48338399 MS.gene97479:intron 40.0%
TGGTTGTTCTTCCAACTACA+AGG + chr7.4:48339335-48339354 None:intergenic 40.0%
! CCATTACTTCTTTTGCAGCA+TGG - chr7.4:48339168-48339187 MS.gene97479:intron 40.0%
! GCTGCAAAAGAAGTAATGGA+AGG + chr7.4:48339167-48339186 None:intergenic 40.0%
! GTGAGTTGGATTGACAACTT+TGG + chr7.4:48338587-48338606 None:intergenic 40.0%
! GTGGACATTTTCCCTGTATT+TGG - chr7.4:48338161-48338180 MS.gene97479:intron 40.0%
AAGTAGGGCCAACAATAAGC+AGG + chr7.4:48337973-48337992 None:intergenic 45.0%
AGTAGGGCCAACAATAAGCA+GGG + chr7.4:48337972-48337991 None:intergenic 45.0%
ATGCTGGCAAATGAAGCTTC+TGG + chr7.4:48339355-48339374 None:intergenic 45.0%
CAGCCAAATAACTGGAAGGT+TGG - chr7.4:48338384-48338403 MS.gene97479:intron 45.0%
CAGTACCAGTTGAGGAAGTA+TGG + chr7.4:48338196-48338215 None:intergenic 45.0%
CTTTGTAGCAGTACCAGTTG+AGG + chr7.4:48338204-48338223 None:intergenic 45.0%
GCATAGAACGAGCTTAGTAG+TGG - chr7.4:48338428-48338447 MS.gene97479:intron 45.0%
GCTTTGTCTGTGAAATGCAG+TGG - chr7.4:48338960-48338979 MS.gene97479:CDS 45.0%
GGCAACAGCTTCCAAATACA+GGG + chr7.4:48338175-48338194 None:intergenic 45.0%
TGGCAACAGCTTCCAAATAC+AGG + chr7.4:48338176-48338195 None:intergenic 45.0%
TGTTGCCATACTTCCTCAAC+TGG - chr7.4:48338188-48338207 MS.gene97479:CDS 45.0%
! CTTTTGCAGCATGGGTTTCT+GGG - chr7.4:48339177-48339196 MS.gene97479:intron 45.0%
! GGTAAATTGGCCTACTTGTC+AGG - chr7.4:48339249-48339268 MS.gene97479:CDS 45.0%
! TCTTTTGCAGCATGGGTTTC+TGG - chr7.4:48339176-48339195 MS.gene97479:intron 45.0%
!! TTAGTTTGCAGTAGTGCTCG+TGG - chr7.4:48338655-48338674 MS.gene97479:intron 45.0%
TCGTGGACAAGCCTGTTTGT+TGG - chr7.4:48338672-48338691 MS.gene97479:intron 50.0%
CCAATGGACAAAGTGGGGCT+CGG + chr7.4:48339030-48339049 None:intergenic 55.0%
CCGAGCCCCACTTTGTCCAT+TGG - chr7.4:48339027-48339046 MS.gene97479:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 48337849 48339376 48337849 ID=MS.gene97479
chr7.4 mRNA 48337849 48339376 48337849 ID=MS.gene97479.t1;Parent=MS.gene97479
chr7.4 exon 48339206 48339376 48339206 ID=MS.gene97479.t1.exon1;Parent=MS.gene97479.t1
chr7.4 CDS 48339206 48339376 48339206 ID=cds.MS.gene97479.t1;Parent=MS.gene97479.t1
chr7.4 exon 48338954 48339069 48338954 ID=MS.gene97479.t1.exon2;Parent=MS.gene97479.t1
chr7.4 CDS 48338954 48339069 48338954 ID=cds.MS.gene97479.t1;Parent=MS.gene97479.t1
chr7.4 exon 48338172 48338245 48338172 ID=MS.gene97479.t1.exon3;Parent=MS.gene97479.t1
chr7.4 CDS 48338172 48338245 48338172 ID=cds.MS.gene97479.t1;Parent=MS.gene97479.t1
chr7.4 exon 48337976 48338039 48337976 ID=MS.gene97479.t1.exon4;Parent=MS.gene97479.t1
chr7.4 CDS 48337976 48338039 48337976 ID=cds.MS.gene97479.t1;Parent=MS.gene97479.t1
chr7.4 exon 48337849 48337885 48337849 ID=MS.gene97479.t1.exon5;Parent=MS.gene97479.t1
chr7.4 CDS 48337849 48337885 48337849 ID=cds.MS.gene97479.t1;Parent=MS.gene97479.t1
Gene Sequence

>MS.gene97479

ATGAAGTTGATATTCTTGGGAAGCTCATTTTCTATTGTATGGTACATGAGGTACCACAAGATTGTTCGTAGATCATATGACAAAGATCAGGATACCTTCCGTCACTATTTTCTAGTATTGCCCTGCTTATTGTTGGCCCTACTTATCAATGAGAAATTCACATTTAAGGAGGTTAGAATGCATTCACTGTTGTCTATCTTCTGTAACTGTTGTGCTGCCATACATGTTTGAATGTTTTTAAGAGATGATGACTTTGCATTAATTTAGCTTTCGTTTGTTGTCCCAAAACTGCTTATACATGGAATAGGTGATGTGGACATTTTCCCTGTATTTGGAAGCTGTTGCCATACTTCCTCAACTGGTACTGCTACAAAGAACTAGAAACATTGACAATTTAACAGGACAATATGTCTTTCTTCTTGGGTAAGCTATGGTCCTTTAACATTATATATTTGCATGATTTCTGTGGCTGTTATTGCATTAGTATTGAAATTAGCAGCTATTATATTCTGGAAGTATTTCAGTTACTGATCAACAGCCAAATAACTGGAAGGTTGGTCTATGTTGATATCTATTTCTGCATAGAACGAGCTTAGTAGTGGTTTCATGCATGATAATGATGATGTAAATCGTAGTTGCAATTTGATACAGTTCTGAGTCATTGAACTAATGCTCTTTAAATAGTGATTCACATGAAGAAAATCATAGCATTTCATGTAACATGTTGTATAGGTTCCAAAGTTGTCAATCCAACTCACCTAGATAAATTATAGCTAATGGGGACAACAAAAGGAATAGAAAAATTATTAGTTTGCAGTAGTGCTCGTGGACAAGCCTGTTTGTTGGCTTCATTGAAACTGATCAATGAGACTGGCCTTCTATTATGATCTTGAGTTGAAAGTTTTTATTTGTTGGAACGAAACTGGCCTTCTATTATGATCTTGAAGCCAAAGTTCTGCTTATTAAGGTTTTCAAGTTTATGCAACTAGCTCTACATATAGTGTGCTATAATAAAAAATAAGCAATGGTAACTTTGTATATCAGAGGAACCCATTTGAACTTGTTTTAGCTTTTAGGCTTTAAGATCTTAGAATTCTGATTCAATAATATTGCTTTGTCTGTGAAATGCAGTGGGTACAGAGCACTATACATTCTCAACTGGGTCTATCGCTACTTTACCGAGCCCCACTTTGTCCATTGGATAAGTATAATTCTGTCTCTTGTTTAATTGTTATCATTGGTTTATGCTCTTTAGACTGGAAAACTTTTCCATCATTGTTAAACATTACAGTGGCTATAAACCTTGTTCTGACTTCCTTCCATTACTTCTTTTGCAGCATGGGTTTCTGGGCTTGTTCAAACACTGCTATATGCTGACTTCTTCTACTACTATTTCCAAAGGTAAATTGGCCTACTTGTCAGGTCCATTTTATTTATGTTAATAATCCTTGTTCGTCTTAGTTTTGAGCATTTGAAAATATTGCCTTGTAGTTGGAAGAACAACCAGAAGCTTCATTTGCCAGCATAG

Protein sequence

>MS.gene97479.t1

MKLIFLGSSFSIVWYMRYHKIVRRSYDKDQDTFRHYFLVLPCLLLALLINEKFTFKEVMWTFSLYLEAVAILPQLVLLQRTRNIDNLTGQYVFLLGGYRALYILNWVYRYFTEPHFVHWITWVSGLVQTLLYADFFYYYFQSWKNNQKLHLPA