AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene98523


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98523.t1 MTR_5g084570 96.000 50 2 0 1 50 1 50 1.17e-28 104
MS.gene98523.t1 MTR_5g084540 100.000 26 0 0 149 174 135 160 1.07e-14 68.2
MS.gene98523.t1 MTR_5g084460 69.643 56 16 1 1 56 1 55 2.73e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 83 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTACCTCTGATGCCAAAA+AGG 0.253882 5.2:-72194829 MS.gene98523:CDS
GTTGTAACCTCCACCATATT+TGG 0.288132 5.2:+72194653 None:intergenic
GAAGTAAACGATGCCAAATA+TGG 0.342429 5.2:-72194666 MS.gene98523:CDS
ACCTGCCATGGCAATGGTAT+TGG 0.432416 5.2:+72194352 None:intergenic
GAACCATAGCCAAGAGGCCT+AGG 0.443599 5.2:+72194892 None:intergenic
CATCCTAGGCCTCTTGGCTA+TGG 0.447886 5.2:-72194895 MS.gene98523:CDS
AATCCTCATCCTAGGCCTCT+TGG 0.458683 5.2:-72194901 MS.gene98523:CDS
GCCAATACCATTGCCATGGC+AGG 0.481585 5.2:-72194353 MS.gene98523:CDS
GGCTACAACCACGGTGGAGG+CGG 0.498062 5.2:-72194399 MS.gene98523:CDS
AGTGTTGTCCTTAGCTTGCA+TGG 0.498460 5.2:+72194305 None:intergenic
AATGGTGGTGGCTACAACCA+CGG 0.509286 5.2:-72194408 MS.gene98523:CDS
GGTGGCTACAACCACGGTGG+AGG 0.512711 5.2:-72194402 MS.gene98523:CDS
AGAAAAGCAATCCTCATCCT+AGG 0.516305 5.2:-72194909 MS.gene98523:CDS
GGATACAACAATGGTGGAGG+TGG 0.518813 5.2:-72194498 MS.gene98523:CDS
GGATACAACAATGGTGGAGG+TGG 0.518813 5.2:-72194540 MS.gene98523:CDS
GGATACAACAATGGTGGAGG+TGG 0.518813 5.2:-72194582 MS.gene98523:CDS
GGAGGTTACAACCACGGCGG+TGG 0.523190 5.2:-72194642 MS.gene98523:CDS
GGATACAACCACGGTGGTGG+TGG 0.524452 5.2:-72194477 MS.gene98523:CDS
GGATACAACCACGGTGGTGG+TGG 0.524452 5.2:-72194519 MS.gene98523:CDS
GGATACAACCACGGTGGTGG+TGG 0.524452 5.2:-72194561 MS.gene98523:CDS
GGTTACAACCACGGTGGTGG+TGG 0.527779 5.2:-72194603 MS.gene98523:CDS
GGCGGTTACAACCACGGTGG+AGG 0.531542 5.2:-72194381 MS.gene98523:CDS
TAGCCAAGAGGCCTAGGATG+AGG 0.538496 5.2:+72194898 None:intergenic
ATTTGTTGCCATGCAAGCTA+AGG 0.539995 5.2:-72194313 MS.gene98523:CDS
GGTTACAACCACGGCGGTGG+TGG 0.544864 5.2:-72194639 MS.gene98523:CDS
GGAGGTGGTTACAATGGTGG+TGG 0.545650 5.2:-72194420 MS.gene98523:CDS
GGAGGTTACAACCACGGTGG+TGG 0.547932 5.2:-72194606 MS.gene98523:CDS
GGTTACAACCACGGTGGAGG+TGG 0.549674 5.2:-72194378 MS.gene98523:CDS
GGTTACAACCATGGTGGAGG+TGG 0.550910 5.2:-72194435 MS.gene98523:CDS
TTCCTCAGAAGTCTCAGCTA+GGG 0.554700 5.2:-72194862 MS.gene98523:CDS
GGTGGTTACAACCATGGTGG+AGG 0.556342 5.2:-72194438 MS.gene98523:CDS
GGTGGATACAACCACGGTGG+TGG 0.558361 5.2:-72194480 MS.gene98523:CDS
GGTGGATACAACCACGGTGG+TGG 0.558361 5.2:-72194522 MS.gene98523:CDS
GGTGGATACAACCACGGTGG+TGG 0.558361 5.2:-72194564 MS.gene98523:CDS
CATGTGAGCAGCACCTGCCA+TGG 0.559419 5.2:+72194340 None:intergenic
TTTCCTCAGAAGTCTCAGCT+AGG 0.560253 5.2:-72194863 MS.gene98523:CDS
GGATACAACAATGGTGGAGG+TGG 0.564967 5.2:-72194456 MS.gene98523:CDS
CACGGCGGTGGTGGTTACAA+TGG 0.571191 5.2:-72194630 MS.gene98523:CDS
AACGATGCCAAATATGGTGG+AGG 0.573315 5.2:-72194660 MS.gene98523:CDS
GGCGGTGGTGGTTACAATGG+TGG 0.594919 5.2:-72194627 MS.gene98523:CDS
CATGGTGGAGGTGGTTACAA+TGG 0.597788 5.2:-72194426 MS.gene98523:CDS
GGTGGATACAACAATGGTGG+AGG 0.598383 5.2:-72194459 MS.gene98523:CDS
GGTGGATACAACAATGGTGG+AGG 0.598383 5.2:-72194501 MS.gene98523:CDS
GGTGGATACAACAATGGTGG+AGG 0.598383 5.2:-72194543 MS.gene98523:CDS
GGTGGATACAACAATGGTGG+AGG 0.598383 5.2:-72194585 MS.gene98523:CDS
GGTGGTGGTTACAATGGTGG+AGG 0.609526 5.2:-72194624 MS.gene98523:CDS
GGTGGAGGTGGTTACAATGG+TGG 0.613370 5.2:-72194423 MS.gene98523:CDS
AGCAGCACCTGCCATGGCAA+TGG 0.620983 5.2:+72194346 None:intergenic
GGTTGCCAATACCATTGCCA+TGG 0.634007 5.2:-72194357 MS.gene98523:CDS
GTCCCTAGCTGAGACTTCTG+AGG 0.645915 5.2:+72194860 None:intergenic
CATTGTAACCACCACCGCCG+TGG 0.646466 5.2:+72194631 None:intergenic
GTAAACGATGCCAAATATGG+TGG 0.647144 5.2:-72194663 MS.gene98523:CDS
CATTGTAACCACCTCCACCA+TGG 0.649002 5.2:+72194427 None:intergenic
TAAGAAGAACCATAGCCAAG+AGG 0.664133 5.2:+72194886 None:intergenic
GGTGGAGGCGGTTACAACCA+CGG 0.674017 5.2:-72194387 MS.gene98523:CDS
GGTGGTGGATACAACAATGG+TGG 0.676280 5.2:-72194462 MS.gene98523:CDS
GGTGGTGGATACAACAATGG+TGG 0.676280 5.2:-72194504 MS.gene98523:CDS
GGTGGTGGATACAACAATGG+TGG 0.676280 5.2:-72194546 MS.gene98523:CDS
GGTGGTGGATACAACAATGG+TGG 0.676280 5.2:-72194588 MS.gene98523:CDS
GGTGGAGGTGGTTACAACCA+TGG 0.679817 5.2:-72194444 MS.gene98523:CDS
GGTTGTAACCGCCTCCACCG+TGG 0.681332 5.2:+72194391 None:intergenic
GGTGGTGGTGGATACAACAA+TGG 0.684345 5.2:-72194465 MS.gene98523:CDS
GGTGGTGGTGGATACAACAA+TGG 0.684345 5.2:-72194507 MS.gene98523:CDS
GGTGGTGGTGGATACAACAA+TGG 0.684345 5.2:-72194549 MS.gene98523:CDS
GGTGGTGGTGGATACAACAA+TGG 0.684345 5.2:-72194591 MS.gene98523:CDS
TATGGTGGAGGTTACAACCA+CGG 0.702708 5.2:-72194648 MS.gene98523:CDS
AATGGTGGAGGTTACAACCA+CGG 0.710899 5.2:-72194612 MS.gene98523:CDS
TGTTGTATCCACCACCACCG+TGG 0.714982 5.2:+72194469 None:intergenic
TGTTGTATCCACCACCACCG+TGG 0.714982 5.2:+72194511 None:intergenic
TGTTGTATCCACCACCACCG+TGG 0.714982 5.2:+72194553 None:intergenic
TGTTGTATCCACCACCACCG+TGG 0.714982 5.2:+72194595 None:intergenic
ATTGGCAACCACCTCCACCG+TGG 0.717584 5.2:+72194370 None:intergenic
GGTGGAGGTGGATACAACCA+CGG 0.728168 5.2:-72194486 MS.gene98523:CDS
GGTGGAGGTGGATACAACCA+CGG 0.728168 5.2:-72194528 MS.gene98523:CDS
GGTGGAGGTGGATACAACCA+CGG 0.728168 5.2:-72194570 MS.gene98523:CDS
GGAGGTGGTTACAACCATGG+TGG 0.740649 5.2:-72194441 MS.gene98523:CDS
GGTGGTGGCTACAACCACGG+TGG 0.783243 5.2:-72194405 MS.gene98523:CDS
GGAGGCGGTTACAACCACGG+TGG 0.797498 5.2:-72194384 MS.gene98523:CDS
GGTGGAGGTTACAACCACGG+TGG 0.812517 5.2:-72194609 MS.gene98523:CDS
GGAGGTGGATACAACCACGG+TGG 0.823069 5.2:-72194483 MS.gene98523:CDS
GGAGGTGGATACAACCACGG+TGG 0.823069 5.2:-72194525 MS.gene98523:CDS
GGAGGTGGATACAACCACGG+TGG 0.823069 5.2:-72194567 MS.gene98523:CDS
GGTGGAGGTTACAACCACGG+CGG 0.825418 5.2:-72194645 MS.gene98523:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATATATAGCTCACCCTTTT+TGG + chr5.2:72194402-72194421 None:intergenic 30.0%
GAAGTAAACGATGCCAAATA+TGG - chr5.2:72194548-72194567 MS.gene98523:CDS 35.0%
AACTACCTCTGATGCCAAAA+AGG - chr5.2:72194385-72194404 MS.gene98523:CDS 40.0%
ACTACCTCTGATGCCAAAAA+GGG - chr5.2:72194386-72194405 MS.gene98523:CDS 40.0%
AGAAAAGCAATCCTCATCCT+AGG - chr5.2:72194305-72194324 MS.gene98523:CDS 40.0%
GTAAACGATGCCAAATATGG+TGG - chr5.2:72194551-72194570 MS.gene98523:CDS 40.0%
GTTGTAACCTCCACCATATT+TGG + chr5.2:72194564-72194583 None:intergenic 40.0%
TAAGAAGAACCATAGCCAAG+AGG + chr5.2:72194331-72194350 None:intergenic 40.0%
! ATTTGTTGCCATGCAAGCTA+AGG - chr5.2:72194901-72194920 MS.gene98523:CDS 40.0%
AACGATGCCAAATATGGTGG+AGG - chr5.2:72194554-72194573 MS.gene98523:CDS 45.0%
TTCCTCAGAAGTCTCAGCTA+GGG - chr5.2:72194352-72194371 MS.gene98523:CDS 45.0%
TTTCCTCAGAAGTCTCAGCT+AGG - chr5.2:72194351-72194370 MS.gene98523:CDS 45.0%
! AATGGTGGAGGTTACAACCA+CGG - chr5.2:72194602-72194621 MS.gene98523:CDS 45.0%
! TATGGTGGAGGTTACAACCA+CGG - chr5.2:72194566-72194585 MS.gene98523:CDS 45.0%
!! AGTGTTGTCCTTAGCTTGCA+TGG + chr5.2:72194912-72194931 None:intergenic 45.0%
AATCCTCATCCTAGGCCTCT+TGG - chr5.2:72194313-72194332 MS.gene98523:CDS 50.0%
ACCTGCCATGGCAATGGTAT+TGG + chr5.2:72194865-72194884 None:intergenic 50.0%
CATTGTAACCACCTCCACCA+TGG + chr5.2:72194790-72194809 None:intergenic 50.0%
GGTGGATACAACAATGGTGG+AGG - chr5.2:72194629-72194648 MS.gene98523:CDS 50.0%
GGTTGCCAATACCATTGCCA+TGG - chr5.2:72194857-72194876 MS.gene98523:CDS 50.0%
! CATGGTGGAGGTGGTTACAA+TGG - chr5.2:72194788-72194807 MS.gene98523:intron 50.0%
! GGATACAACAATGGTGGAGG+TGG - chr5.2:72194632-72194651 MS.gene98523:CDS 50.0%
!! AATGGTGGTGGCTACAACCA+CGG - chr5.2:72194806-72194825 MS.gene98523:intron 50.0%
!! CTCACCCTTTTTGGCATCAG+AGG + chr5.2:72194393-72194412 None:intergenic 50.0%
!! GGTGGTGGATACAACAATGG+TGG - chr5.2:72194626-72194645 MS.gene98523:CDS 50.0%
!! GGTGGTGGTGGATACAACAA+TGG - chr5.2:72194623-72194642 MS.gene98523:CDS 50.0%
CATCCTAGGCCTCTTGGCTA+TGG - chr5.2:72194319-72194338 MS.gene98523:CDS 55.0%
GAACCATAGCCAAGAGGCCT+AGG + chr5.2:72194325-72194344 None:intergenic 55.0%
GCCAATACCATTGCCATGGC+AGG - chr5.2:72194861-72194880 MS.gene98523:CDS 55.0%
GGAGGTGGTTACAACCATGG+TGG - chr5.2:72194773-72194792 MS.gene98523:intron 55.0%
GGAGGTGGTTACAATGGTGG+TGG - chr5.2:72194794-72194813 MS.gene98523:intron 55.0%
GGTGGAGGTGGATACAACCA+CGG - chr5.2:72194644-72194663 MS.gene98523:CDS 55.0%
GGTGGAGGTGGTTACAACCA+TGG - chr5.2:72194770-72194789 MS.gene98523:intron 55.0%
GGTGGAGGTGGTTACAATGG+TGG - chr5.2:72194791-72194810 MS.gene98523:intron 55.0%
GGTGGTTACAACCATGGTGG+AGG - chr5.2:72194776-72194795 MS.gene98523:intron 55.0%
GTCCCTAGCTGAGACTTCTG+AGG + chr5.2:72194357-72194376 None:intergenic 55.0%
TAGCCAAGAGGCCTAGGATG+AGG + chr5.2:72194319-72194338 None:intergenic 55.0%
TGTTGTATCCACCACCACCG+TGG + chr5.2:72194622-72194641 None:intergenic 55.0%
! GGTGGTGGTTACAATGGTGG+AGG - chr5.2:72194590-72194609 MS.gene98523:CDS 55.0%
! GGTTACAACCATGGTGGAGG+TGG - chr5.2:72194779-72194798 MS.gene98523:intron 55.0%
AGCAGCACCTGCCATGGCAA+TGG + chr5.2:72194871-72194890 None:intergenic 60.0%
ATTGGCAACCACCTCCACCG+TGG + chr5.2:72194847-72194866 None:intergenic 60.0%
CATGTGAGCAGCACCTGCCA+TGG + chr5.2:72194877-72194896 None:intergenic 60.0%
CATTGTAACCACCACCGCCG+TGG + chr5.2:72194586-72194605 None:intergenic 60.0%
GGAGGTGGATACAACCACGG+TGG - chr5.2:72194647-72194666 MS.gene98523:CDS 60.0%
GGAGGTTACAACCACGGTGG+TGG - chr5.2:72194608-72194627 MS.gene98523:CDS 60.0%
GGTGGAGGCGGTTACAACCA+CGG - chr5.2:72194827-72194846 MS.gene98523:intron 60.0%
GGTGGAGGTTACAACCACGG+CGG - chr5.2:72194569-72194588 MS.gene98523:CDS 60.0%
GGTGGAGGTTACAACCACGG+TGG - chr5.2:72194569-72194588 MS.gene98523:CDS 60.0%
GGTGGATACAACCACGGTGG+TGG - chr5.2:72194650-72194669 MS.gene98523:CDS 60.0%
GGTTACAACCACGGTGGAGG+TGG - chr5.2:72194836-72194855 MS.gene98523:CDS 60.0%
! CACGGCGGTGGTGGTTACAA+TGG - chr5.2:72194584-72194603 MS.gene98523:CDS 60.0%
! GGATACAACCACGGTGGTGG+TGG - chr5.2:72194653-72194672 MS.gene98523:CDS 60.0%
! GGCGGTGGTGGTTACAATGG+TGG - chr5.2:72194587-72194606 MS.gene98523:CDS 60.0%
! GGTTACAACCACGGTGGTGG+TGG - chr5.2:72194611-72194630 MS.gene98523:CDS 60.0%
GGAGGCGGTTACAACCACGG+TGG - chr5.2:72194830-72194849 MS.gene98523:CDS 65.0%
GGAGGTTACAACCACGGCGG+TGG - chr5.2:72194572-72194591 MS.gene98523:CDS 65.0%
GGCGGTTACAACCACGGTGG+AGG - chr5.2:72194833-72194852 MS.gene98523:CDS 65.0%
GGCTACAACCACGGTGGAGG+CGG - chr5.2:72194815-72194834 MS.gene98523:intron 65.0%
GGTGGCTACAACCACGGTGG+AGG - chr5.2:72194812-72194831 MS.gene98523:intron 65.0%
GGTTACAACCACGGCGGTGG+TGG - chr5.2:72194575-72194594 MS.gene98523:CDS 65.0%
GGTTGTAACCGCCTCCACCG+TGG + chr5.2:72194826-72194845 None:intergenic 65.0%
! GGTGGTGGCTACAACCACGG+TGG - chr5.2:72194809-72194828 MS.gene98523:intron 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 72194296 72194940 72194296 ID=MS.gene98523
chr5.2 mRNA 72194296 72194940 72194296 ID=MS.gene98523.t1;Parent=MS.gene98523
chr5.2 exon 72194829 72194940 72194829 ID=MS.gene98523.t1.exon1;Parent=MS.gene98523.t1
chr5.2 CDS 72194829 72194940 72194829 ID=cds.MS.gene98523.t1;Parent=MS.gene98523.t1
chr5.2 exon 72194296 72194708 72194296 ID=MS.gene98523.t1.exon2;Parent=MS.gene98523.t1
chr5.2 CDS 72194296 72194708 72194296 ID=cds.MS.gene98523.t1;Parent=MS.gene98523.t1
Gene Sequence

>MS.gene98523

ATGGATTCGAGAAAAGCAATCCTCATCCTAGGCCTCTTGGCTATGGTTCTTCTTATTTCCTCAGAAGTCTCAGCTAGGGACTTAACTGAAACTACCTCTGATGCCAAAAAGGGTGAGCTATATATTATTTTTCTTTATAAATACTTCACGAGCATTTTCTATATTGTTTAAATTCTTTTCTAGTTAATTATATATAAAGTAATGATTCATATTATATGTGCTGTGTTTGCAGAAGTTGTTGAAAAGACTAATGAAGTAAACGATGCCAAATATGGTGGAGGTTACAACCACGGCGGTGGTGGTTACAATGGTGGAGGTTACAACCACGGTGGTGGTGGATACAACAATGGTGGAGGTGGATACAACCACGGTGGTGGTGGATACAACAATGGTGGAGGTGGATACAACCACGGTGGTGGTGGATACAACAATGGTGGAGGTGGATACAACCACGGTGGTGGTGGATACAACAATGGTGGAGGTGGTTACAACCATGGTGGAGGTGGTTACAATGGTGGTGGCTACAACCACGGTGGAGGCGGTTACAACCACGGTGGAGGTGGTTGCCAATACCATTGCCATGGCAGGTGCTGCTCACATGCTGAATTTGTTGCCATGCAAGCTAAGGACAACACTCAGAATTGA

Protein sequence

>MS.gene98523.t1

MDSRKAILILGLLAMVLLISSEVSARDLTETTSDAKKEVVEKTNEVNDAKYGGGYNHGGGGYNGGGYNHGGGGYNNGGGGYNHGGGGYNNGGGGYNHGGGGYNNGGGGYNHGGGGYNNGGGGYNHGGGGYNGGGYNHGGGGYNHGGGGCQYHCHGRCCSHAEFVAMQAKDNTQN