AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene98699


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98699.t1 MTR_7g070530 98.561 278 4 0 1 278 232 509 0.0 584
MS.gene98699.t1 MTR_4g013290 75.986 279 61 4 1 278 236 509 3.16e-150 430
MS.gene98699.t1 MTR_3g031200 70.039 257 70 2 28 278 239 494 4.38e-133 385
MS.gene98699.t1 MTR_7g101130 31.660 259 140 11 19 276 242 464 2.85e-30 118
MS.gene98699.t1 MTR_1g064690 25.581 258 138 11 23 277 259 465 5.56e-19 86.7
MS.gene98699.t1 MTR_4g093860 26.267 217 89 7 64 278 241 388 4.08e-14 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98699.t1 AT5G01710 70.213 282 80 3 1 278 232 513 6.55e-144 414
MS.gene98699.t1 AT3G53400 29.185 233 113 6 54 277 274 463 4.61e-25 103
MS.gene98699.t1 AT5G03190 26.172 256 138 6 25 277 241 448 1.80e-23 99.4
MS.gene98699.t1 AT5G03190 26.172 256 138 6 25 277 265 472 2.16e-23 99.4

Find 62 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAACAGTTTCATGTATATA+AGG 0.288284 7.1:-31387641 None:intergenic
GGGGAGCATGTTGAGGTTAA+AGG 0.290336 7.1:+31388097 MS.gene98699:CDS
ATTCCGAGATTGTTTGAAAC+TGG 0.306986 7.1:+31388286 MS.gene98699:CDS
CTCCTTATGAAAATGCTTAT+CGG 0.309551 7.1:-31387987 None:intergenic
TGAAATGTCTTATTCTGTTT+AGG 0.310557 7.1:-31387947 None:intergenic
CTAGTATAGGTTCTTGGTTT+AGG 0.310622 7.1:+31387915 MS.gene98699:CDS
ATGGTTCTAGTATAGGTTCT+TGG 0.339618 7.1:+31387909 MS.gene98699:CDS
CTTTGGTTCCTTATGCTGCT+TGG 0.341853 7.1:+31388032 MS.gene98699:CDS
GAATGGAAATTGTTATGTTC+CGG 0.346460 7.1:+31387718 MS.gene98699:CDS
GATTATTATTCGAATTTGAA+TGG 0.351180 7.1:+31387701 MS.gene98699:CDS
AATGGAAATTGTTATGTTCC+GGG 0.355231 7.1:+31387719 MS.gene98699:CDS
TATAAAGTATCTTTCTTCAA+TGG 0.364263 7.1:+31387832 MS.gene98699:CDS
TTTGGTTCCTTATGCTGCTT+GGG 0.368027 7.1:+31388033 MS.gene98699:CDS
AAAAGGAACCATTGAAGCCT+TGG 0.374178 7.1:+31387783 MS.gene98699:CDS
TTCTTGAATGTCATTACAAT+AGG 0.383434 7.1:+31388335 MS.gene98699:CDS
AATGTAATCCAAGGCTTCAA+TGG 0.393903 7.1:-31387791 None:intergenic
GTGGAATTCTTGTTCATCAA+TGG 0.398427 7.1:+31388446 MS.gene98699:CDS
TCCGGGATATAAGAAAGATT+TGG 0.413671 7.1:+31387736 MS.gene98699:CDS
TGCTGAGCCATTGATTGAAA+AGG 0.415041 7.1:+31387766 MS.gene98699:CDS
TGGGGAGGTTGAGAAGATAA+AGG 0.427305 7.1:+31388171 MS.gene98699:CDS
AGGGAAGGGGTTTGATGGGG+AGG 0.444105 7.1:+31388156 MS.gene98699:CDS
GGGGAGGTTGAGAAGATAAA+GGG 0.448534 7.1:+31388172 MS.gene98699:CDS
TCGATAGGGAAGGGGTTTGA+TGG 0.450774 7.1:+31388151 MS.gene98699:CDS
AGCCTTGGATTACATTGAAA+AGG 0.451168 7.1:+31387798 MS.gene98699:CDS
TTCAATTCTCTTAGACAAAG+TGG 0.459178 7.1:+31388427 MS.gene98699:CDS
ACCAAATCTTTCTTATATCC+CGG 0.461264 7.1:-31387737 None:intergenic
AGAAGCTATGGTTCTAGTAT+AGG 0.468833 7.1:+31387902 MS.gene98699:CDS
GTTTGAGATTCATCGTGATC+CGG 0.474286 7.1:+31388075 MS.gene98699:CDS
TTCCGAGATTGTTTGAAACT+GGG 0.479979 7.1:+31388287 MS.gene98699:CDS
AGGAGTATGGGTTGAAGAAA+GGG 0.480321 7.1:+31388005 MS.gene98699:CDS
AAGGAGTATGGGTTGAAGAA+AGG 0.486257 7.1:+31388004 MS.gene98699:CDS
GATAGGGAAGGGGTTTGATG+GGG 0.487355 7.1:+31388153 MS.gene98699:CDS
ATTCAACCTTTGCGGTCGAT+AGG 0.494435 7.1:+31388136 MS.gene98699:CDS
TTTGAGATTCATCGTGATCC+GGG 0.502701 7.1:+31388076 MS.gene98699:CDS
AGAGATGTTGTCCTGGGCAG+AGG 0.507298 7.1:+31388362 MS.gene98699:CDS
GTTGAAGAAAGGGCTTACTT+TGG 0.517477 7.1:+31388015 MS.gene98699:CDS
CGATAGGGAAGGGGTTTGAT+GGG 0.520667 7.1:+31388152 MS.gene98699:CDS
TTGAATGTCATTACAATAGG+TGG 0.524370 7.1:+31388338 MS.gene98699:CDS
ACCTTTGCGGTCGATAGGGA+AGG 0.531001 7.1:+31388141 MS.gene98699:CDS
TTCAACCTTTGCGGTCGATA+GGG 0.534099 7.1:+31388137 MS.gene98699:CDS
TGGGTAGGATTCAACCTTTG+CGG 0.534681 7.1:+31388128 MS.gene98699:CDS
CATGTTGAGGTTAAAGGAAG+GGG 0.537032 7.1:+31388103 MS.gene98699:CDS
AGGTATATATATGTTGATGT+TGG 0.544575 7.1:+31387875 MS.gene98699:CDS
GAAAACTTATGATCAATGCT+TGG 0.549157 7.1:+31388399 MS.gene98699:CDS
TTAACCTCAACATGCTCCCC+CGG 0.551682 7.1:-31388094 None:intergenic
GCCCCAGTTTCAAACAATCT+CGG 0.554909 7.1:-31388289 None:intergenic
TCCGAGATTGTTTGAAACTG+GGG 0.555012 7.1:+31388288 MS.gene98699:CDS
AGGTGGCAGAGATGTTGTCC+TGG 0.557939 7.1:+31388355 MS.gene98699:CDS
AGCATGTTGAGGTTAAAGGA+AGG 0.559095 7.1:+31388101 MS.gene98699:CDS
CTTTGCGGTCGATAGGGAAG+GGG 0.559268 7.1:+31388143 MS.gene98699:CDS
CCTTTGCGGTCGATAGGGAA+GGG 0.563419 7.1:+31388142 MS.gene98699:CDS
GATGTTGGAGCTAGAAGCTA+TGG 0.569584 7.1:+31387890 MS.gene98699:CDS
TTGAGATTCATCGTGATCCG+GGG 0.576008 7.1:+31388077 MS.gene98699:CDS
TACTTCGAACTCCTCTGCCC+AGG 0.584824 7.1:-31388373 None:intergenic
GCATGTTGAGGTTAAAGGAA+GGG 0.598675 7.1:+31388102 MS.gene98699:CDS
GGTGGCAGAGATGTTGTCCT+GGG 0.606062 7.1:+31388356 MS.gene98699:CDS
ATGTTGAGGTTAAAGGAAGG+GGG 0.624038 7.1:+31388104 MS.gene98699:CDS
CCCTTCCCTATCGACCGCAA+AGG 0.632938 7.1:-31388142 None:intergenic
GGGAGGTTGAGAAGATAAAG+GGG 0.638084 7.1:+31388173 MS.gene98699:CDS
TGAGATTCATCGTGATCCGG+GGG 0.648064 7.1:+31388078 MS.gene98699:CDS
TGTGATGAAAATGGATGTCG+AGG 0.663557 7.1:+31388246 MS.gene98699:CDS
GTGATGAAAATGGATGTCGA+GGG 0.763196 7.1:+31388247 MS.gene98699:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGATATAAGTTTCAAAAAT+AGG + chr7.1:31387855-31387874 MS.gene98699:CDS 15.0%
!! AAAACAGTTTCATGTATATA+AGG - chr7.1:31387644-31387663 None:intergenic 20.0%
!! GATTATTATTCGAATTTGAA+TGG + chr7.1:31387701-31387720 MS.gene98699:CDS 20.0%
!!! TATAAAGTATCTTTCTTCAA+TGG + chr7.1:31387832-31387851 MS.gene98699:CDS 20.0%
! AGGTATATATATGTTGATGT+TGG + chr7.1:31387875-31387894 MS.gene98699:CDS 25.0%
! TTCTTGAATGTCATTACAAT+AGG + chr7.1:31388335-31388354 MS.gene98699:CDS 25.0%
!! TGAAATGTCTTATTCTGTTT+AGG - chr7.1:31387950-31387969 None:intergenic 25.0%
!!! TGATTTTGTTGTGATGAAAA+TGG + chr7.1:31388237-31388256 MS.gene98699:CDS 25.0%
AATGGAAATTGTTATGTTCC+GGG + chr7.1:31387719-31387738 MS.gene98699:CDS 30.0%
ACCAAATCTTTCTTATATCC+CGG - chr7.1:31387740-31387759 None:intergenic 30.0%
CTCCTTATGAAAATGCTTAT+CGG - chr7.1:31387990-31388009 None:intergenic 30.0%
GAAAACTTATGATCAATGCT+TGG + chr7.1:31388399-31388418 MS.gene98699:CDS 30.0%
GAATGGAAATTGTTATGTTC+CGG + chr7.1:31387718-31387737 MS.gene98699:CDS 30.0%
TTCAATTCTCTTAGACAAAG+TGG + chr7.1:31388427-31388446 MS.gene98699:CDS 30.0%
TTGAATGTCATTACAATAGG+TGG + chr7.1:31388338-31388357 MS.gene98699:CDS 30.0%
! AAGCATTTTCATAAGGAGTA+TGG + chr7.1:31387992-31388011 MS.gene98699:CDS 30.0%
! AATGGTTCCTTTTCAATCAA+TGG - chr7.1:31387776-31387795 None:intergenic 30.0%
! TTCCTTTTCAATGTAATCCA+AGG - chr7.1:31387803-31387822 None:intergenic 30.0%
!! AGCATTTTCATAAGGAGTAT+GGG + chr7.1:31387993-31388012 MS.gene98699:CDS 30.0%
AATGTAATCCAAGGCTTCAA+TGG - chr7.1:31387794-31387813 None:intergenic 35.0%
AGCCTTGGATTACATTGAAA+AGG + chr7.1:31387798-31387817 MS.gene98699:CDS 35.0%
CTAGTATAGGTTCTTGGTTT+AGG + chr7.1:31387915-31387934 MS.gene98699:CDS 35.0%
GTGGAATTCTTGTTCATCAA+TGG + chr7.1:31388446-31388465 MS.gene98699:CDS 35.0%
TCCGGGATATAAGAAAGATT+TGG + chr7.1:31387736-31387755 MS.gene98699:CDS 35.0%
! AGCCGATAAGCATTTTCATA+AGG + chr7.1:31387985-31388004 MS.gene98699:CDS 35.0%
! ATTCCGAGATTGTTTGAAAC+TGG + chr7.1:31388286-31388305 MS.gene98699:CDS 35.0%
! TTCCGAGATTGTTTGAAACT+GGG + chr7.1:31388287-31388306 MS.gene98699:CDS 35.0%
!! AGAAGCTATGGTTCTAGTAT+AGG + chr7.1:31387902-31387921 MS.gene98699:CDS 35.0%
!! ATGGTTCTAGTATAGGTTCT+TGG + chr7.1:31387909-31387928 MS.gene98699:CDS 35.0%
AAAAGGAACCATTGAAGCCT+TGG + chr7.1:31387783-31387802 MS.gene98699:CDS 40.0%
AGCATGTTGAGGTTAAAGGA+AGG + chr7.1:31388101-31388120 MS.gene98699:CDS 40.0%
ATGTTGAGGTTAAAGGAAGG+GGG + chr7.1:31388104-31388123 MS.gene98699:CDS 40.0%
CATGTTGAGGTTAAAGGAAG+GGG + chr7.1:31388103-31388122 MS.gene98699:CDS 40.0%
GCATGTTGAGGTTAAAGGAA+GGG + chr7.1:31388102-31388121 MS.gene98699:CDS 40.0%
GTGATGAAAATGGATGTCGA+GGG + chr7.1:31388247-31388266 MS.gene98699:CDS 40.0%
GTTGAAGAAAGGGCTTACTT+TGG + chr7.1:31388015-31388034 MS.gene98699:CDS 40.0%
TGTGATGAAAATGGATGTCG+AGG + chr7.1:31388246-31388265 MS.gene98699:CDS 40.0%
! AAGGAGTATGGGTTGAAGAA+AGG + chr7.1:31388004-31388023 MS.gene98699:CDS 40.0%
! AGGAGTATGGGTTGAAGAAA+GGG + chr7.1:31388005-31388024 MS.gene98699:CDS 40.0%
! GTTTGAGATTCATCGTGATC+CGG + chr7.1:31388075-31388094 MS.gene98699:CDS 40.0%
! TCCGAGATTGTTTGAAACTG+GGG + chr7.1:31388288-31388307 MS.gene98699:CDS 40.0%
! TGCTGAGCCATTGATTGAAA+AGG + chr7.1:31387766-31387785 MS.gene98699:CDS 40.0%
! TTTGAGATTCATCGTGATCC+GGG + chr7.1:31388076-31388095 MS.gene98699:CDS 40.0%
! TTTGGTTCCTTATGCTGCTT+GGG + chr7.1:31388033-31388052 MS.gene98699:CDS 40.0%
! TTTTTCACCCAAGCAGCATA+AGG - chr7.1:31388043-31388062 None:intergenic 40.0%
!! AGGGGTTTGATTTTGCGAAT+TGG + chr7.1:31388191-31388210 MS.gene98699:CDS 40.0%
ATTCAACCTTTGCGGTCGAT+AGG + chr7.1:31388136-31388155 MS.gene98699:CDS 45.0%
GATGTTGGAGCTAGAAGCTA+TGG + chr7.1:31387890-31387909 MS.gene98699:CDS 45.0%
GCCCCAGTTTCAAACAATCT+CGG - chr7.1:31388292-31388311 None:intergenic 45.0%
GGGAGGTTGAGAAGATAAAG+GGG + chr7.1:31388173-31388192 MS.gene98699:CDS 45.0%
GGGGAGGTTGAGAAGATAAA+GGG + chr7.1:31388172-31388191 MS.gene98699:CDS 45.0%
TGGGGAGGTTGAGAAGATAA+AGG + chr7.1:31388171-31388190 MS.gene98699:CDS 45.0%
TGGGTAGGATTCAACCTTTG+CGG + chr7.1:31388128-31388147 MS.gene98699:CDS 45.0%
TTCAACCTTTGCGGTCGATA+GGG + chr7.1:31388137-31388156 MS.gene98699:CDS 45.0%
TTGAGATTCATCGTGATCCG+GGG + chr7.1:31388077-31388096 MS.gene98699:CDS 45.0%
! CTTTGGTTCCTTATGCTGCT+TGG + chr7.1:31388032-31388051 MS.gene98699:CDS 45.0%
CGATAGGGAAGGGGTTTGAT+GGG + chr7.1:31388152-31388171 MS.gene98699:CDS 50.0%
GAGGTTAAAGGAAGGGGGAT+GGG + chr7.1:31388109-31388128 MS.gene98699:CDS 50.0%
GATAGGGAAGGGGTTTGATG+GGG + chr7.1:31388153-31388172 MS.gene98699:CDS 50.0%
TCGATAGGGAAGGGGTTTGA+TGG + chr7.1:31388151-31388170 MS.gene98699:CDS 50.0%
TGAGATTCATCGTGATCCGG+GGG + chr7.1:31388078-31388097 MS.gene98699:CDS 50.0%
TGAGGTTAAAGGAAGGGGGA+TGG + chr7.1:31388108-31388127 MS.gene98699:CDS 50.0%
TTAAAGGAAGGGGGATGGGT+AGG + chr7.1:31388113-31388132 MS.gene98699:CDS 50.0%
TTAACCTCAACATGCTCCCC+CGG - chr7.1:31388097-31388116 None:intergenic 50.0%
! GGGGAGCATGTTGAGGTTAA+AGG + chr7.1:31388097-31388116 MS.gene98699:CDS 50.0%
ACCTTTGCGGTCGATAGGGA+AGG + chr7.1:31388141-31388160 MS.gene98699:CDS 55.0%
AGAGATGTTGTCCTGGGCAG+AGG + chr7.1:31388362-31388381 MS.gene98699:CDS 55.0%
AGGTGGCAGAGATGTTGTCC+TGG + chr7.1:31388355-31388374 MS.gene98699:CDS 55.0%
CCTTTGCGGTCGATAGGGAA+GGG + chr7.1:31388142-31388161 MS.gene98699:CDS 55.0%
CTTTGCGGTCGATAGGGAAG+GGG + chr7.1:31388143-31388162 MS.gene98699:CDS 55.0%
GGTGGCAGAGATGTTGTCCT+GGG + chr7.1:31388356-31388375 MS.gene98699:CDS 55.0%
TACTTCGAACTCCTCTGCCC+AGG - chr7.1:31388376-31388395 None:intergenic 55.0%
AGGGAAGGGGTTTGATGGGG+AGG + chr7.1:31388156-31388175 MS.gene98699:CDS 60.0%
CCCTTCCCTATCGACCGCAA+AGG - chr7.1:31388145-31388164 None:intergenic 60.0%
! TGATCCGGGGGAGCATGTTG+AGG + chr7.1:31388090-31388109 MS.gene98699:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 31387638 31388474 31387638 ID=MS.gene98699
chr7.1 mRNA 31387638 31388474 31387638 ID=MS.gene98699.t1;Parent=MS.gene98699
chr7.1 exon 31387638 31388474 31387638 ID=MS.gene98699.t1.exon1;Parent=MS.gene98699.t1
chr7.1 CDS 31387638 31388474 31387638 ID=cds.MS.gene98699.t1;Parent=MS.gene98699.t1
Gene Sequence

>MS.gene98699

ATGCCTTATATACATGAAACTGTTTTGAAGAATGAGTGTGTTGATTATGAAAAATTTGATAGTGATTATTATTCGAATTTGAATGGAAATTGTTATGTTCCGGGATATAAGAAAGATTTGGTTAGAATTGCTGAGCCATTGATTGAAAAGGAACCATTGAAGCCTTGGATTACATTGAAAAGGAATTTGATGAATATAAAGTATCTTTCTTCAATGGTTGATATAAGTTTCAAAAATAGGTATATATATGTTGATGTTGGAGCTAGAAGCTATGGTTCTAGTATAGGTTCTTGGTTTAGGAAACAGTATCCTAAACAGAATAAGACATTTCATGTGTATGCGATTGAAGCCGATAAGCATTTTCATAAGGAGTATGGGTTGAAGAAAGGGCTTACTTTGGTTCCTTATGCTGCTTGGGTGAAAAATGAGACTTTAGCGTTTGAGATTCATCGTGATCCGGGGGAGCATGTTGAGGTTAAAGGAAGGGGGATGGGTAGGATTCAACCTTTGCGGTCGATAGGGAAGGGGTTTGATGGGGAGGTTGAGAAGATAAAGGGGTTTGATTTTGCGAATTGGTTGAAGAAGACTGTTTCGAAGAATGATTTTGTTGTGATGAAAATGGATGTCGAGGGTACTGAGTTTGATCTTATTCCGAGATTGTTTGAAACTGGGGCGATTTGTTTGATAGATGAGATTTTTCTTGAATGTCATTACAATAGGTGGCAGAGATGTTGTCCTGGGCAGAGGAGTTCGAAGTATGAGAAAACTTATGATCAATGCTTGGAGCTCTTCAATTCTCTTAGACAAAGTGGAATTCTTGTTCATCAATGGTTTTAA

Protein sequence

>MS.gene98699.t1

MPYIHETVLKNECVDYEKFDSDYYSNLNGNCYVPGYKKDLVRIAEPLIEKEPLKPWITLKRNLMNIKYLSSMVDISFKNRYIYVDVGARSYGSSIGSWFRKQYPKQNKTFHVYAIEADKHFHKEYGLKKGLTLVPYAAWVKNETLAFEIHRDPGEHVEVKGRGMGRIQPLRSIGKGFDGEVEKIKGFDFANWLKKTVSKNDFVVMKMDVEGTEFDLIPRLFETGAICLIDEIFLECHYNRWQRCCPGQRSSKYEKTYDQCLELFNSLRQSGILVHQWF