AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048400.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048400.01.T01 MTR_6g007680 67.961 103 32 1 36 138 37 138 1.69e-45 147
MsG0080048400.01.T01 MTR_6g007670 67.327 101 31 2 37 136 143 242 7.18e-40 135
MsG0080048400.01.T01 MTR_6g007670 56.566 99 39 2 36 134 5 99 6.87e-33 117
MsG0080048400.01.T01 MTR_3g078120 54.930 71 30 2 44 112 62 132 2.26e-23 90.5
MsG0080048400.01.T01 MTR_5g024730 34.444 90 55 2 10 97 50 137 1.53e-11 60.8
MsG0080048400.01.T01 MTR_5g066250 43.750 64 33 1 37 97 129 192 2.83e-11 61.2
MsG0080048400.01.T01 MTR_2g436100 43.396 53 30 0 45 97 99 151 7.58e-11 59.7
MsG0080048400.01.T01 MTR_6g007737 46.296 54 29 0 45 98 139 192 8.40e-11 59.7
MsG0080048400.01.T01 MTR_3g100400 42.857 63 33 1 38 97 126 188 9.01e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048400.01.T01 AT3G48030 38.571 70 42 1 30 98 171 240 2.12e-11 61.6
MsG0080048400.01.T01 AT3G48030 38.571 70 42 1 30 98 93 162 2.26e-11 60.8
MsG0080048400.01.T01 AT1G71980 60.976 41 15 1 57 97 224 263 3.85e-11 60.8
MsG0080048400.01.T01 AT4G28890 42.857 56 32 0 42 97 100 155 4.10e-11 60.8
MsG0080048400.01.T01 AT1G23980 46.296 54 29 0 45 98 124 177 6.77e-11 60.1
MsG0080048400.01.T01 AT5G01880 42.105 57 33 0 42 98 82 138 6.80e-11 58.2
MsG0080048400.01.T01 AT5G05810 42.857 63 33 1 38 97 116 178 8.62e-11 59.7

Find 32 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTATGTTGCAAAAGAAAA+TGG 0.230340 contig302end:+28694 MsG0080048400.01.T01:CDS
TTGTCCAATTTGTAGATCTA+TGG 0.257672 contig302end:+28845 MsG0080048400.01.T01:CDS
GTTTGGAGAATTTCACTATT+GGG 0.290326 contig302end:+28743 MsG0080048400.01.T01:CDS
AGTGCAAACAAAGTTGTTAT+AGG 0.302873 contig302end:+28880 MsG0080048400.01.T01:CDS
TGTTTGGAGAATTTCACTAT+TGG 0.324990 contig302end:+28742 MsG0080048400.01.T01:CDS
TTTAGATTGTGCAATTTGTT+TGG 0.325499 contig302end:+28726 MsG0080048400.01.T01:CDS
CCAACCATAGATCTACAAAT+TGG 0.331126 contig302end:-28849 None:intergenic
CCTTCAACTATTAGTGACTT+TGG 0.350680 contig302end:+31697 MsG0080048400.01.T01:CDS
GAGCAACATCAATGGTTGAT+AGG 0.368262 contig302end:+28625 MsG0080048400.01.T01:CDS
AAGCTTCTCTAAATCAACTT+TGG 0.410464 contig302end:-28662 None:intergenic
AACAAATTGCACAATCTAAA+GGG 0.417519 contig302end:-28723 None:intergenic
TTAAGACCCTCAATCTTAAG+TGG 0.417996 contig302end:-28192 None:intergenic
AGATCTACAAATTGGACAAA+TGG 0.421145 contig302end:-28841 None:intergenic
ATGCTCAATGTGTGGATCAA+TGG 0.439509 contig302end:+28806 MsG0080048400.01.T01:CDS
AAACAAATTGCACAATCTAA+AGG 0.442477 contig302end:-28724 None:intergenic
CCAAAGTCACTAATAGTTGA+AGG 0.451506 contig302end:-31697 None:intergenic
TTTCTTCTATCAGCATCTGC+AGG 0.460426 contig302end:-31673 None:intergenic
TTCTCTAAATCAACTTTGGA+AGG 0.474683 contig302end:-28658 None:intergenic
AGGATGTAGAGTACCTGTAT+AGG 0.475824 contig302end:-29058 None:intergenic
GTGGGGTCCACTTAAGATTG+AGG 0.483782 contig302end:+28185 MsG0080048400.01.T01:CDS
TGGGGTCCACTTAAGATTGA+GGG 0.488421 contig302end:+28186 MsG0080048400.01.T01:CDS
TTCTTCTATCAGCATCTGCA+GGG 0.490983 contig302end:-31672 None:intergenic
CCAATTTGTAGATCTATGGT+TGG 0.498208 contig302end:+28849 MsG0080048400.01.T01:CDS
AGCAACATCAATGGTTGATA+GGG 0.499654 contig302end:+28626 MsG0080048400.01.T01:CDS
TTTGGAGAATTTCACTATTG+GGG 0.528111 contig302end:+28744 MsG0080048400.01.T01:CDS
AGAATCACAAGATCCTATAC+AGG 0.543727 contig302end:+29045 MsG0080048400.01.T01:CDS
TGAAAATTGTGCTTGCAAGT+AGG 0.575339 contig302end:-28784 None:intergenic
TCTTCTATCAGCATCTGCAG+GGG 0.582156 contig302end:-31671 None:intergenic
ACTAGGAAGAGCAACATCAA+TGG 0.584676 contig302end:+28617 MsG0080048400.01.T01:intron
AAATAGGTACAGTTGAAGAT+CGG 0.592836 contig302end:+29005 MsG0080048400.01.T01:intron
TCTGCAGGGGTTCTCAAACA+TGG 0.598714 contig302end:-31658 None:intergenic
TTTGCAACATAATCATAACA+TGG 0.646690 contig302end:-28685 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTTATAAATTAATTAA+TGG - contig302end:30484-30503 None:intergenic 0.0%
!! AAAATAAAATAAGTAATGTA+CGG + contig302end:30731-30750 MsG0080048400.01.T01:intron 10.0%
!! ATAATAGATAATAATTTATG+AGG - contig302end:30312-30331 None:intergenic 10.0%
!! TTTATAAATTAATTAATGGA+CGG - contig302end:30480-30499 None:intergenic 10.0%
!!! GATATGTTTTTTTATTTTTT+TGG + contig302end:29763-29782 MsG0080048400.01.T01:intron 10.0%
!!! TAAATTAGAGTATTAATAAA+TGG + contig302end:29210-29229 MsG0080048400.01.T01:intron 10.0%
!! AAATAAAATAAGTAATGTAC+GGG + contig302end:30732-30751 MsG0080048400.01.T01:intron 15.0%
!! AAATAATATATACAACCTAA+AGG - contig302end:29854-29873 None:intergenic 15.0%
!! ACATATTTATTATGATTTAG+TGG + contig302end:30602-30621 MsG0080048400.01.T01:intron 15.0%
!! GTAACATATCTATAAATATA+TGG - contig302end:30288-30307 None:intergenic 15.0%
!! TCTTATGAAAATTATGTAAA+AGG + contig302end:31483-31502 MsG0080048400.01.T01:intron 15.0%
!!! AATGATTATTTTATTGAAAG+TGG + contig302end:28161-28180 MsG0080048400.01.T01:CDS 15.0%
!!! TATTGTAAAAAAAAGTATCT+TGG - contig302end:30075-30094 None:intergenic 15.0%
!!! TTATTTTTTCCTTTGTTTTA+AGG - contig302end:29656-29675 None:intergenic 15.0%
!! AACTGTAGATAGAAAAAAAA+CGG + contig302end:30183-30202 MsG0080048400.01.T01:intron 20.0%
!! AATAAAATAAGTAATGTACG+GGG + contig302end:30733-30752 MsG0080048400.01.T01:intron 20.0%
!! AATTTATTAGTAATCTCCAA+AGG - contig302end:29081-29100 None:intergenic 20.0%
!! ATCATTCCATGTAATTAAAT+CGG - contig302end:31123-31142 None:intergenic 20.0%
!! CATTGTGGAAAAAAAAATTA+GGG - contig302end:28243-28262 None:intergenic 20.0%
!! CTTATTAATGAAGTAATTAG+TGG - contig302end:31337-31356 None:intergenic 20.0%
!! TGAATATAATGTAATAACCA+TGG - contig302end:29830-29849 None:intergenic 20.0%
!! TTACATTATATTCATCCTTT+AGG + contig302end:29836-29855 MsG0080048400.01.T01:intron 20.0%
!!! AAATTTTAGCAAACATGAAA+AGG + contig302end:30117-30136 MsG0080048400.01.T01:intron 20.0%
!!! AATAAATGGTATGTTTTAGA+GGG + contig302end:29224-29243 MsG0080048400.01.T01:intron 20.0%
!!! AATTTGAGACAATCAATTAT+TGG + contig302end:30903-30922 MsG0080048400.01.T01:intron 20.0%
!!! ATATGTGTACTTTTTTTTTG+AGG + contig302end:30538-30557 MsG0080048400.01.T01:intron 20.0%
!!! ATTTAACCGATTTAATTACA+TGG + contig302end:31114-31133 MsG0080048400.01.T01:intron 20.0%
!!! CTAATTTTTTTTTCCACAAT+GGG + contig302end:28242-28261 MsG0080048400.01.T01:intron 20.0%
!!! GAATATAGTTTCTGAAAATT+AGG - contig302end:30266-30285 None:intergenic 20.0%
!!! TAATAAATGGTATGTTTTAG+AGG + contig302end:29223-29242 MsG0080048400.01.T01:intron 20.0%
!!! TAATTTTTTTTTCCACAATG+GGG + contig302end:28243-28262 MsG0080048400.01.T01:intron 20.0%
!!! TATGTGTACTTTTTTTTTGA+GGG + contig302end:30539-30558 MsG0080048400.01.T01:intron 20.0%
! AAACAAATTGCACAATCTAA+AGG - contig302end:28727-28746 None:intergenic 25.0%
! AAAGTTGTTATAGGTAACAA+TGG + contig302end:28889-28908 MsG0080048400.01.T01:intron 25.0%
! AACAAATTGCACAATCTAAA+GGG - contig302end:28726-28745 None:intergenic 25.0%
! AAGTAATTAGTGGAATAGTT+AGG - contig302end:31327-31346 None:intergenic 25.0%
! AATATGACTTATATGTGATG+TGG + contig302end:30805-30824 MsG0080048400.01.T01:intron 25.0%
! AGCTCTATTATAATATTTGC+AGG + contig302end:28534-28553 MsG0080048400.01.T01:intron 25.0%
! AGTTATATGATTATGAGACT+AGG + contig302end:28600-28619 MsG0080048400.01.T01:intron 25.0%
! ATAAGACAAACTCTTAACAT+TGG - contig302end:28412-28431 None:intergenic 25.0%
! ATACCAAGAAACATAGAATA+AGG + contig302end:30092-30111 MsG0080048400.01.T01:intron 25.0%
! ATGTCGAATATTGTTCTTAA+AGG - contig302end:31096-31115 None:intergenic 25.0%
! ATTGTTTAGTATTAGTACCA+TGG + contig302end:29810-29829 MsG0080048400.01.T01:intron 25.0%
! CCATTGTGGAAAAAAAAATT+AGG - contig302end:28244-28263 None:intergenic 25.0%
! CTCTATAAAAAACTTGATAG+AGG - contig302end:29492-29511 None:intergenic 25.0%
! GATTATGTTGCAAAAGAAAA+TGG + contig302end:28694-28713 MsG0080048400.01.T01:CDS 25.0%
! GATTTGTCTAAATATGCTAA+TGG - contig302end:30051-30070 None:intergenic 25.0%
! GGTAAATATGATTAGACATT+AGG + contig302end:31189-31208 MsG0080048400.01.T01:intron 25.0%
! TAAGACAAACTCTTAACATT+GGG - contig302end:28411-28430 None:intergenic 25.0%
! TAGAAGATCAATATGCAAAA+TGG - contig302end:29112-29131 None:intergenic 25.0%
! TGAAATATGAAACTAGAAGT+TGG - contig302end:29187-29206 None:intergenic 25.0%
! TTTGCAACATAATCATAACA+TGG - contig302end:28688-28707 None:intergenic 25.0%
!! GTTTAGAATTGTTTACAACT+AGG + contig302end:31505-31524 MsG0080048400.01.T01:intron 25.0%
!! TATTTTATTGAAAGTGGTGT+GGG + contig302end:28167-28186 MsG0080048400.01.T01:CDS 25.0%
!! TCAAAATGCTAACCAATTTT+AGG - contig302end:31603-31622 None:intergenic 25.0%
!! TTTAGATTGTGCAATTTGTT+TGG + contig302end:28726-28745 MsG0080048400.01.T01:CDS 25.0%
!!! AATTTTTTTTTCCACAATGG+GGG + contig302end:28244-28263 MsG0080048400.01.T01:intron 25.0%
!!! ATATTTTGAATGATGAGCAA+TGG + contig302end:29598-29617 MsG0080048400.01.T01:intron 25.0%
!!! CAAAAAGTAGTTTGTTAGAT+TGG + contig302end:29342-29361 MsG0080048400.01.T01:intron 25.0%
!!! CCTAATTTTTTTTTCCACAA+TGG + contig302end:28241-28260 MsG0080048400.01.T01:intron 25.0%
!!! GATGATTTGTATGTCAATTT+TGG - contig302end:31546-31565 None:intergenic 25.0%
!!! TTATTTTATTGAAAGTGGTG+TGG + contig302end:28166-28185 MsG0080048400.01.T01:CDS 25.0%
!!! TTTTTTATAGAGCGACTTAT+TGG + contig302end:29500-29519 MsG0080048400.01.T01:intron 25.0%
AAAAGAATGAGAGTAGTGAA+AGG - contig302end:29969-29988 None:intergenic 30.0%
AAATAGGTACAGTTGAAGAT+CGG + contig302end:29005-29024 MsG0080048400.01.T01:intron 30.0%
AAGCTTCTCTAAATCAACTT+TGG - contig302end:28665-28684 None:intergenic 30.0%
ACTTTAAACTGCATTTGAGA+CGG + contig302end:31431-31450 MsG0080048400.01.T01:intron 30.0%
AGATCTACAAATTGGACAAA+TGG - contig302end:28844-28863 None:intergenic 30.0%
AGTGCAAACAAAGTTGTTAT+AGG + contig302end:28880-28899 MsG0080048400.01.T01:CDS 30.0%
ATAATCATATAACTCTCAGC+AGG - contig302end:28594-28613 None:intergenic 30.0%
ATCTATTATCTCCTCTCATT+AGG - contig302end:30991-31010 None:intergenic 30.0%
CAGGAAAACATATACGAAAT+AGG - contig302end:28575-28594 None:intergenic 30.0%
CTACAATTATGACAATTGAG+AGG + contig302end:30229-30248 MsG0080048400.01.T01:intron 30.0%
GAATGGATAAATAAGAGAGT+TGG - contig302end:31302-31321 None:intergenic 30.0%
GTTTGGAGAATTTCACTATT+GGG + contig302end:28743-28762 MsG0080048400.01.T01:CDS 30.0%
TATAGTGAACAGTTTCTCTT+TGG + contig302end:28358-28377 MsG0080048400.01.T01:intron 30.0%
TGACCTTATTCTATGTTTCT+TGG - contig302end:30098-30117 None:intergenic 30.0%
TGCATAGAAACTTGTACTTA+TGG + contig302end:29276-29295 MsG0080048400.01.T01:intron 30.0%
TGCTAATGGTTAAATTTGTG+TGG - contig302end:30037-30056 None:intergenic 30.0%
TGTGATTCTACTTCAAATGT+TGG - contig302end:28977-28996 None:intergenic 30.0%
TGTTTGGAGAATTTCACTAT+TGG + contig302end:28742-28761 MsG0080048400.01.T01:CDS 30.0%
TTCGATACACCTTAAAACAA+AGG + contig302end:29644-29663 MsG0080048400.01.T01:intron 30.0%
TTCTCTAAATCAACTTTGGA+AGG - contig302end:28661-28680 None:intergenic 30.0%
TTGTCCAATTTGTAGATCTA+TGG + contig302end:28845-28864 MsG0080048400.01.T01:CDS 30.0%
TTTGGAGAATTTCACTATTG+GGG + contig302end:28744-28763 MsG0080048400.01.T01:CDS 30.0%
! ATTTTATTGAAAGTGGTGTG+GGG + contig302end:28168-28187 MsG0080048400.01.T01:CDS 30.0%
!! TGTATGTCAATTTTGGCATT+GGG - contig302end:31539-31558 None:intergenic 30.0%
!! TTGTATGTCAATTTTGGCAT+TGG - contig302end:31540-31559 None:intergenic 30.0%
AACCAACTAAGCAATCATTG+AGG - contig302end:29916-29935 None:intergenic 35.0%
AGAATCACAAGATCCTATAC+AGG + contig302end:29045-29064 MsG0080048400.01.T01:CDS 35.0%
AGTGGAATAGTTAGGTAGAA+TGG - contig302end:31319-31338 None:intergenic 35.0%
ATAGTAAAGACCCTACAATG+TGG + contig302end:28291-28310 MsG0080048400.01.T01:intron 35.0%
ATCACTACTTCATCGTCTAA+GGG - contig302end:30935-30954 None:intergenic 35.0%
ATGGACACACATTTCTTCAA+TGG + contig302end:29295-29314 MsG0080048400.01.T01:intron 35.0%
ATGGTTAAATTTGTGTGGAG+TGG - contig302end:30032-30051 None:intergenic 35.0%
CATCATGCCAAAAACTTGAT+AGG - contig302end:31165-31184 None:intergenic 35.0%
CCAAAGTCACTAATAGTTGA+AGG - contig302end:31700-31719 None:intergenic 35.0%
CCAACCATAGATCTACAAAT+TGG - contig302end:28852-28871 None:intergenic 35.0%
CCAATTTGTAGATCTATGGT+TGG + contig302end:28849-28868 MsG0080048400.01.T01:CDS 35.0%
CCTTCAACTATTAGTGACTT+TGG + contig302end:31697-31716 MsG0080048400.01.T01:CDS 35.0%
CTACAGTTATGACAATTGAC+AGG - contig302end:30171-30190 None:intergenic 35.0%
CTATCAGACTCTATTCGTTT+AGG - contig302end:31070-31089 None:intergenic 35.0%
GAGAGGAGATAATAGATGTT+AGG + contig302end:30994-31013 MsG0080048400.01.T01:intron 35.0%
GCAATGGTGTCTTTATGTAT+TGG + contig302end:29614-29633 MsG0080048400.01.T01:intron 35.0%
GTTATGACAATTGACAGGTT+TGG - contig302end:30166-30185 None:intergenic 35.0%
TACGGGGTAAAAATGAGTAA+GGG + contig302end:30749-30768 MsG0080048400.01.T01:intron 35.0%
TATCACTACTTCATCGTCTA+AGG - contig302end:30936-30955 None:intergenic 35.0%
TGAAAATTGTGCTTGCAAGT+AGG - contig302end:28787-28806 None:intergenic 35.0%
TGGATAAATAAGAGAGTTGG+TGG - contig302end:31299-31318 None:intergenic 35.0%
TTAAGACCCTCAATCTTAAG+TGG - contig302end:28195-28214 None:intergenic 35.0%
! ACCAATTTTAGGCTTCAACT+TGG - contig302end:31592-31611 None:intergenic 35.0%
! AGCAACATCAATGGTTGATA+GGG + contig302end:28626-28645 MsG0080048400.01.T01:CDS 35.0%
! ATCATTGAGGTGCTATGTAA+TGG - contig302end:29903-29922 None:intergenic 35.0%
! ATTTTGTCATCATCCAGCTA+GGG + contig302end:31035-31054 MsG0080048400.01.T01:intron 35.0%
! CAATTTTAGGCTTCAACTTG+GGG - contig302end:31590-31609 None:intergenic 35.0%
! CAATTTTCATGCTCAATGTG+TGG + contig302end:28798-28817 MsG0080048400.01.T01:CDS 35.0%
! CCAATTTTAGGCTTCAACTT+GGG - contig302end:31591-31610 None:intergenic 35.0%
! GGGAAAGTACATTTACATCA+AGG - contig302end:29390-29409 None:intergenic 35.0%
! GGTCTTTACTATTTAAGACC+CGG - contig302end:28283-28302 None:intergenic 35.0%
! TATTTTGTCATCATCCAGCT+AGG + contig302end:31034-31053 MsG0080048400.01.T01:intron 35.0%
! TCGTGATCCTATCAAGTTTT+TGG + contig302end:31155-31174 MsG0080048400.01.T01:intron 35.0%
! TTTAAGACCCGGTACTTAAT+AGG - contig302end:28272-28291 None:intergenic 35.0%
!! GAATCACAAGAGCACTAAAT+AGG + contig302end:28989-29008 MsG0080048400.01.T01:intron 35.0%
!! GTTTCTCTTTGGCTGATTTT+TGG + contig302end:28369-28388 MsG0080048400.01.T01:intron 35.0%
!!! AAGTTTTTGGCATGATGAGA+AGG + contig302end:31168-31187 MsG0080048400.01.T01:intron 35.0%
!!! TTTTGAGTTCATGAAGTCAG+TGG + contig302end:31617-31636 MsG0080048400.01.T01:intron 35.0%
ACTAGGAAGAGCAACATCAA+TGG + contig302end:28617-28636 MsG0080048400.01.T01:intron 40.0%
AGTATCATAGGCCTAATGAG+AGG + contig302end:30977-30996 MsG0080048400.01.T01:intron 40.0%
ATGCTCAATGTGTGGATCAA+TGG + contig302end:28806-28825 MsG0080048400.01.T01:CDS 40.0%
CACCTCAATGATTGCTTAGT+TGG + contig302end:29911-29930 MsG0080048400.01.T01:intron 40.0%
GAGCAACATCAATGGTTGAT+AGG + contig302end:28625-28644 MsG0080048400.01.T01:CDS 40.0%
GCAAGTTTAGCACTTTGTTG+GGG - contig302end:31568-31587 None:intergenic 40.0%
GGCAAGTTTAGCACTTTGTT+GGG - contig302end:31569-31588 None:intergenic 40.0%
GTACGGGGTAAAAATGAGTA+AGG + contig302end:30748-30767 MsG0080048400.01.T01:intron 40.0%
TACAGGTACTCTACATCCTT+TGG + contig302end:29062-29081 MsG0080048400.01.T01:intron 40.0%
TAGGGTCTCTTAAAGACTCA+GGG - contig302end:28225-28244 None:intergenic 40.0%
TTAGGCCTATGATACTCGTA+AGG - contig302end:30973-30992 None:intergenic 40.0%
TTAGGGTCTCTTAAAGACTC+AGG - contig302end:28226-28245 None:intergenic 40.0%
TTCTTCTATCAGCATCTGCA+GGG - contig302end:31675-31694 None:intergenic 40.0%
TTTCTTCTATCAGCATCTGC+AGG - contig302end:31676-31695 None:intergenic 40.0%
! AGGATGTAGAGTACCTGTAT+AGG - contig302end:29061-29080 None:intergenic 40.0%
! CCCAAGTTGAAGCCTAAAAT+TGG + contig302end:31588-31607 MsG0080048400.01.T01:intron 40.0%
! GGAAGAGAAAGCATAGAAAG+TGG + contig302end:28910-28929 MsG0080048400.01.T01:intron 40.0%
! GGTTCTCAGCTTTTGCTAAA+GGG + contig302end:29932-29951 MsG0080048400.01.T01:intron 40.0%
! TGGTTCTCAGCTTTTGCTAA+AGG + contig302end:29931-29950 MsG0080048400.01.T01:intron 40.0%
!! ACTAAGAGCATCCACATTGT+AGG - contig302end:28305-28324 None:intergenic 40.0%
!! ATTTGCAGGACTAGTGCTTT+TGG + contig302end:28548-28567 MsG0080048400.01.T01:intron 40.0%
!! CTAAGAGCATCCACATTGTA+GGG - contig302end:28304-28323 None:intergenic 40.0%
!! GCATCATTGAGTGTTGAGAA+TGG + contig302end:28501-28520 MsG0080048400.01.T01:intron 40.0%
CTTAATAGGTACCCCCATTG+TGG - contig302end:28258-28277 None:intergenic 45.0%
GGGCAAGTTTAGCACTTTGT+TGG - contig302end:31570-31589 None:intergenic 45.0%
TAGGTTTGTCAAACCCTAGC+TGG - contig302end:31051-31070 None:intergenic 45.0%
TCTTCTATCAGCATCTGCAG+GGG - contig302end:31674-31693 None:intergenic 45.0%
TGGGGTCCACTTAAGATTGA+GGG + contig302end:28186-28205 MsG0080048400.01.T01:CDS 45.0%
! GTGTGGATCAATGGCTTTTG+AGG + contig302end:28815-28834 MsG0080048400.01.T01:CDS 45.0%
!! TGGGGGTACCTATTAAGTAC+CGG + contig302end:28261-28280 MsG0080048400.01.T01:intron 45.0%
!! AAATAAAATTGTATTAAAAA+TGG - contig302end:29411-29430 None:intergenic 5.0%
!! AATAAAATTGTATTAAAAAT+GGG - contig302end:29410-29429 None:intergenic 5.0%
ACAAGAAACAGTGAGCCAGG+AGG - contig302end:28330-28349 None:intergenic 50.0%
AGCACAAGAAACAGTGAGCC+AGG - contig302end:28333-28352 None:intergenic 50.0%
GCGCTCCTTACGAGTATCAT+AGG + contig302end:30965-30984 MsG0080048400.01.T01:intron 50.0%
GGATGCTCTTAGTTACCTCC+TGG + contig302end:28312-28331 MsG0080048400.01.T01:intron 50.0%
GTGGGGTCCACTTAAGATTG+AGG + contig302end:28185-28204 MsG0080048400.01.T01:CDS 50.0%
TCTGCAGGGGTTCTCAAACA+TGG - contig302end:31661-31680 None:intergenic 50.0%
!! GGGGGTACCTATTAAGTACC+GGG + contig302end:28262-28281 MsG0080048400.01.T01:intron 50.0%
Chromosome Type Strat End Strand Name
contig302end gene 28135 31735 28135 ID=MsG0080048400.01;Name=MsG0080048400.01
contig302end mRNA 28135 31735 28135 ID=MsG0080048400.01.T01;Parent=MsG0080048400.01;Name=MsG0080048400.01.T01;_AED=0.61;_eAED=0.61;_QI=0|0|0|0.25|1|1|4|0|171
contig302end exon 28135 28215 28135 ID=MsG0080048400.01.T01:exon:3594;Parent=MsG0080048400.01.T01
contig302end exon 28622 28901 28622 ID=MsG0080048400.01.T01:exon:3595;Parent=MsG0080048400.01.T01
contig302end exon 29011 29066 29011 ID=MsG0080048400.01.T01:exon:3596;Parent=MsG0080048400.01.T01
contig302end exon 31637 31735 31637 ID=MsG0080048400.01.T01:exon:3597;Parent=MsG0080048400.01.T01
contig302end CDS 28135 28215 28135 ID=MsG0080048400.01.T01:cds;Parent=MsG0080048400.01.T01
contig302end CDS 28622 28901 28622 ID=MsG0080048400.01.T01:cds;Parent=MsG0080048400.01.T01
contig302end CDS 29011 29066 29011 ID=MsG0080048400.01.T01:cds;Parent=MsG0080048400.01.T01
contig302end CDS 31637 31735 31637 ID=MsG0080048400.01.T01:cds;Parent=MsG0080048400.01.T01
Gene Sequence

>MsG0080048400.01.T01

ATGAAAAACAACAAAACAACTCAAATAATGATTATTTTATTGAAAGTGGTGTGGGGTCCACTTAAGATTGAGGGTCTTAAAGAAGAGCAACATCAATGGTTGATAGGGAAGAAGAAGCCTTCCAAAGTTGATTTAGAGAAGCTTCCATGTTATGATTATGTTGCAAAAGAAAATGGAAGTAACCCTTTAGATTGTGCAATTTGTTTGGAGAATTTCACTATTGGGGATAAGTGTAGATTGTTACCTACTTGCAAGCACAATTTTCATGCTCAATGTGTGGATCAATGGCTTTTGAGGATTCCATTTGTCCAATTTGTAGATCTATGGTTGGTTTCTCATAGTGCAAACAAAGTTGTTATAGGTACAGTTGAAGATCGGTTTGAAGTAGAATCACAAGAATCACAAGATCCTATACAGTGGCAACAAGATCAACAAGAGCCATGTTTGAGAACCCCTGCAGATGCTGATAGAAGAAATCCTTCAACTATTAGTGACTTTGGACTACTTTCATTGTAG
Protein sequence

>MsG0080048400.01.T01

MKNNKTTQIMIILLKVVWGPLKIEGLKEEQHQWLIGKKKPSKVDLEKLPCYDYVAKENGSNPLDCAICLENFTIGDKCRLLPTCKHNFHAQCVDQWLLRIPFVQFVDLWLVSHSANKVVIGTVEDRFEVESQESQDPIQWQQDQQEPCLRTPADADRRNPSTISDFGLLSL*