AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043037.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043037.01.T01 MTR_8g027885 93.902 82 5 0 1 82 396 477 9.63e-48 159
MsG0880043037.01.T01 MTR_8g027870 93.902 82 5 0 1 82 396 477 9.63e-48 159
MsG0880043037.01.T01 MTR_8g008990 65.000 80 28 0 1 80 392 471 2.34e-31 115
MsG0880043037.01.T01 MTR_7g117170 68.831 77 24 0 1 77 399 475 1.52e-29 110
MsG0880043037.01.T01 MTR_8g009063 61.250 80 31 0 1 80 397 476 4.52e-28 106
MsG0880043037.01.T01 MTR_8g009055 61.250 80 31 0 1 80 390 469 1.85e-27 104
MsG0880043037.01.T01 MTR_1g019510 58.750 80 33 0 1 80 387 466 3.67e-27 103
MsG0880043037.01.T01 MTR_8g009050 61.250 80 31 0 1 80 390 469 5.38e-27 103
MsG0880043037.01.T01 MTR_6g005600 68.421 76 24 0 1 76 391 466 1.52e-26 102
MsG0880043037.01.T01 MTR_1g019520 57.500 80 34 0 1 80 136 215 1.62e-25 95.5
MsG0880043037.01.T01 MTR_8g008970 58.750 80 33 0 1 80 712 791 4.94e-25 98.2
MsG0880043037.01.T01 MTR_8g008970 54.878 82 37 0 2 83 389 470 2.52e-23 93.2
MsG0880043037.01.T01 MTR_8g009067 49.412 85 43 0 2 86 389 473 4.44e-23 92.4
MsG0880043037.01.T01 MTR_8g009067 49.412 85 43 0 2 86 271 355 5.01e-23 91.3
MsG0880043037.01.T01 MTR_8g009840 60.256 78 27 2 2 78 452 526 2.43e-22 90.5
MsG0880043037.01.T01 MTR_8g009073 51.899 79 38 0 2 80 389 467 3.08e-22 89.7
MsG0880043037.01.T01 MTR_8g009073 51.899 79 38 0 2 80 260 338 3.23e-22 89.0
MsG0880043037.01.T01 MTR_8g012635 51.316 76 37 0 1 76 395 470 4.81e-21 86.7
MsG0880043037.01.T01 MTR_0184s0040 53.846 78 36 0 1 78 404 481 4.86e-21 86.7
MsG0880043037.01.T01 MTR_0078s0190 53.846 78 36 0 1 78 404 481 4.86e-21 86.7
MsG0880043037.01.T01 MTR_7g046490 48.750 80 41 0 1 80 387 466 4.81e-20 83.6
MsG0880043037.01.T01 MTR_6g488280 49.367 79 39 1 1 78 387 465 7.70e-20 83.2
MsG0880043037.01.T01 MTR_0428s0010 49.367 79 39 1 1 78 387 465 7.70e-20 83.2
MsG0880043037.01.T01 MTR_8g012630 52.632 76 36 0 1 76 339 414 8.11e-20 82.8
MsG0880043037.01.T01 MTR_0184s0030 55.405 74 33 0 1 74 391 464 2.40e-19 81.6
MsG0880043037.01.T01 MTR_6g023665 47.561 82 42 1 1 81 394 475 4.73e-19 80.9
MsG0880043037.01.T01 MTR_8g012650 50.000 76 38 0 1 76 395 470 8.68e-19 80.1
MsG0880043037.01.T01 MTR_0184s0010 47.436 78 41 0 1 78 18 95 4.78e-18 73.2
MsG0880043037.01.T01 MTR_8g006730 53.247 77 35 1 1 76 389 465 9.94e-18 77.4
MsG0880043037.01.T01 MTR_8g006260 45.238 84 46 0 2 85 394 477 4.83e-17 75.1
MsG0880043037.01.T01 MTR_8g012730 47.368 76 40 0 1 76 232 307 1.63e-16 73.2
MsG0880043037.01.T01 MTR_8g006270 51.282 78 36 2 1 77 387 463 1.65e-16 73.9
MsG0880043037.01.T01 MTR_8g006710 50.667 75 36 1 1 74 388 462 2.29e-16 73.2
MsG0880043037.01.T01 MTR_8g012745 41.860 86 49 1 1 86 393 477 4.87e-16 72.4
MsG0880043037.01.T01 MTR_6g005640 71.739 46 13 0 1 46 1 46 9.31e-16 66.6
MsG0880043037.01.T01 MTR_0078s0060 48.000 75 38 1 1 74 388 462 2.01e-14 67.8
MsG0880043037.01.T01 MTR_8g077590 42.105 76 44 0 1 76 390 465 2.00e-13 65.1
MsG0880043037.01.T01 MTR_8g465670 39.759 83 45 2 1 78 389 471 8.13e-13 63.2
MsG0880043037.01.T01 MTR_7g047030 44.156 77 42 1 1 76 389 465 1.00e-12 63.2
MsG0880043037.01.T01 MTR_7g047270 44.156 77 42 1 1 76 389 465 1.00e-12 63.2
MsG0880043037.01.T01 MTR_5g019580 40.789 76 43 1 1 74 400 475 7.00e-12 60.5
MsG0880043037.01.T01 MTR_5g019580 40.789 76 43 1 1 74 424 499 7.04e-12 60.5
MsG0880043037.01.T01 MTR_0078s0010 40.741 81 47 1 1 80 389 469 1.31e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043037.01.T01 AT4G01070 52.941 85 40 0 1 85 391 475 4.15e-25 98.2
MsG0880043037.01.T01 AT1G01420 49.367 79 39 1 1 79 391 468 1.51e-18 79.7
MsG0880043037.01.T01 AT1G01420 49.367 79 39 1 1 79 394 471 1.51e-18 79.7

Find 20 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGACCAAAAGTTGGTGAAAA+TGG 0.199004 8:-18004404 MsG0880043037.01.T01:CDS
AACAAAGCAAATATGTGAAT+TGG 0.358779 8:-18004249 MsG0880043037.01.T01:CDS
GACCAAAAGTTGGTGAAAAT+GGG 0.373513 8:-18004403 MsG0880043037.01.T01:CDS
AAGTGTTGTGAAGAGGTTAA+TGG 0.406621 8:-18004357 MsG0880043037.01.T01:CDS
AGTAATGCTCTAAAGGAAAA+CGG 0.424259 8:-18004278 MsG0880043037.01.T01:CDS
GTGAAAATGGGGTGGTTCAA+AGG 0.426938 8:-18004391 MsG0880043037.01.T01:CDS
GTAATGCTCTAAAGGAAAAC+GGG 0.445068 8:-18004277 MsG0880043037.01.T01:CDS
AAAATAAAGGCAAGGGTAAG+AGG 0.455635 8:+18004154 None:intergenic
TGCAGCTAGTAATGCTCTAA+AGG 0.458717 8:-18004285 MsG0880043037.01.T01:CDS
TATGTGAATTGGCTCTCAAA+TGG 0.460975 8:-18004238 MsG0880043037.01.T01:CDS
TGAAAATGGGGTGGTTCAAA+GGG 0.474371 8:-18004390 MsG0880043037.01.T01:CDS
GTTAATGGTAGGAGAAGAAG+AGG 0.489578 8:-18004342 MsG0880043037.01.T01:CDS
GGAAGAAATACATTGAAAGA+AGG 0.490399 8:+18004175 None:intergenic
AAAGATTCGTTATAGAATGA+AGG 0.516245 8:-18004318 MsG0880043037.01.T01:CDS
AAATTGCAAGTGTTGTGAAG+AGG 0.574727 8:-18004364 MsG0880043037.01.T01:CDS
GTTGTGAAGAGGTTAATGGT+AGG 0.584786 8:-18004353 MsG0880043037.01.T01:CDS
GTGGCTATTAGACCAAAAGT+TGG 0.591467 8:-18004413 MsG0880043037.01.T01:CDS
ACCAAAAGTTGGTGAAAATG+GGG 0.645113 8:-18004402 MsG0880043037.01.T01:CDS
AAAAGTTGGTGAAAATGGGG+TGG 0.681286 8:-18004399 MsG0880043037.01.T01:CDS
GAATTGGCTCTCAAATGGAA+AGG 0.691832 8:-18004233 MsG0880043037.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAGATTCGTTATAGAATGA+AGG - Chr8:18004276-18004295 MsG0880043037.01.T01:CDS 25.0%
! AACAAAGCAAATATGTGAAT+TGG - Chr8:18004345-18004364 MsG0880043037.01.T01:CDS 25.0%
! ATTGAGTGAAAAAGTTAAAG+TGG - Chr8:18004162-18004181 MsG0880043037.01.T01:CDS 25.0%
GGAAGAAATACATTGAAAGA+AGG + Chr8:18004422-18004441 None:intergenic 30.0%
!! AGTAATGCTCTAAAGGAAAA+CGG - Chr8:18004316-18004335 MsG0880043037.01.T01:CDS 30.0%
AAAATAAAGGCAAGGGTAAG+AGG + Chr8:18004443-18004462 None:intergenic 35.0%
! AAATTGCAAGTGTTGTGAAG+AGG - Chr8:18004230-18004249 MsG0880043037.01.T01:CDS 35.0%
! AAGTGTTGTGAAGAGGTTAA+TGG - Chr8:18004237-18004256 MsG0880043037.01.T01:CDS 35.0%
! TATGTGAATTGGCTCTCAAA+TGG - Chr8:18004356-18004375 MsG0880043037.01.T01:CDS 35.0%
!! ACCAAAAGTTGGTGAAAATG+GGG - Chr8:18004192-18004211 MsG0880043037.01.T01:CDS 35.0%
!! AGACCAAAAGTTGGTGAAAA+TGG - Chr8:18004190-18004209 MsG0880043037.01.T01:CDS 35.0%
!! GACCAAAAGTTGGTGAAAAT+GGG - Chr8:18004191-18004210 MsG0880043037.01.T01:CDS 35.0%
!! GTAATGCTCTAAAGGAAAAC+GGG - Chr8:18004317-18004336 MsG0880043037.01.T01:CDS 35.0%
GTGGCTATTAGACCAAAAGT+TGG - Chr8:18004181-18004200 MsG0880043037.01.T01:CDS 40.0%
GTTAATGGTAGGAGAAGAAG+AGG - Chr8:18004252-18004271 MsG0880043037.01.T01:CDS 40.0%
GTTGTGAAGAGGTTAATGGT+AGG - Chr8:18004241-18004260 MsG0880043037.01.T01:CDS 40.0%
TGAAAATGGGGTGGTTCAAA+GGG - Chr8:18004204-18004223 MsG0880043037.01.T01:CDS 40.0%
TGCAGCTAGTAATGCTCTAA+AGG - Chr8:18004309-18004328 MsG0880043037.01.T01:CDS 40.0%
! ACCCCATTTTCACCAACTTT+TGG + Chr8:18004196-18004215 None:intergenic 40.0%
! GAATTGGCTCTCAAATGGAA+AGG - Chr8:18004361-18004380 MsG0880043037.01.T01:CDS 40.0%
!! AAAAGTTGGTGAAAATGGGG+TGG - Chr8:18004195-18004214 MsG0880043037.01.T01:CDS 40.0%
GTGAAAATGGGGTGGTTCAA+AGG - Chr8:18004203-18004222 MsG0880043037.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr8 gene 18004148 18004468 18004148 ID=MsG0880043037.01;Name=MsG0880043037.01
Chr8 mRNA 18004148 18004468 18004148 ID=MsG0880043037.01.T01;Parent=MsG0880043037.01;Name=MsG0880043037.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|106
Chr8 exon 18004148 18004468 18004148 ID=MsG0880043037.01.T01:exon:3558;Parent=MsG0880043037.01.T01
Chr8 CDS 18004148 18004468 18004148 ID=MsG0880043037.01.T01:cds;Parent=MsG0880043037.01.T01
Gene Sequence

>MsG0880043037.01.T01

ATGAATGCTGTTTTATTGAGTGAAAAAGTTAAAGTGGCTATTAGACCAAAAGTTGGTGAAAATGGGGTGGTTCAAAGGGAAGAAATTGCAAGTGTTGTGAAGAGGTTAATGGTAGGAGAAGAAGAGGAAAAGATTCGTTATAGAATGAAGGATCTCAAAGATGCAGCTAGTAATGCTCTAAAGGAAAACGGGTCTTCAACAAAGCAAATATGTGAATTGGCTCTCAAATGGAAAGGTGTTACAATACAAATTGAAGTTGCATTTCATTTATCCTTCTTTCAATGTATTTCTTCCTCTTACCCTTGCCTTTATTTTTGTTAG

Protein sequence

>MsG0880043037.01.T01

MNAVLLSEKVKVAIRPKVGENGVVQREEIASVVKRLMVGEEEEKIRYRMKDLKDAASNALKENGSSTKQICELALKWKGVTIQIEVAFHLSFFQCISSSYPCLYFC*