AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021896.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021896.01.T01 MTR_4g078485 82.143 84 15 0 23 106 49 132 7.17e-41 135
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021896.01.T01 AT1G65032 63.014 73 27 0 37 109 12 84 7.58e-27 95.9

Find 30 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAATTTCAAATTCTAATT+AGG 0.130089 4:+65754117 None:intergenic
CCTAATTAGAATTTGAAATT+AGG 0.182424 4:-65754117 MsG0480021896.01.T01:three_prime_UTR
AAAGCATACGGTATTCAAAA+TGG 0.318000 4:-65754234 MsG0480021896.01.T01:three_prime_UTR
CTGAGGTACGTGCAATGTTT+TGG 0.338129 4:-65754450 MsG0480021896.01.T01:CDS
AGTATTCAGCAGTATCAATT+AGG 0.338566 4:+65754389 None:intergenic
GAATGGGAAAGGGTTTGATA+TGG 0.351995 4:-65754593 MsG0480021896.01.T01:CDS
ATAAATGAAAGTTTAACTTA+AGG 0.371812 4:-65754068 MsG0480021896.01.T01:three_prime_UTR
CTCATTATAAGCATACAATA+AGG 0.424983 4:+65754147 None:intergenic
ACTAATTGCGACGAGGGAAA+TGG 0.425687 4:+65754307 None:intergenic
AACATACTCTACATTGGATT+CGG 0.431126 4:+65754183 None:intergenic
GCAGAGGATCTGCATGGAAT+AGG 0.436458 4:-65754564 MsG0480021896.01.T01:CDS
ACTGACATTCGCTGCAAGAC+TGG 0.449305 4:-65754482 MsG0480021896.01.T01:CDS
GGAAATGGAAGGTGTTGATC+AGG 0.482869 4:+65754322 None:intergenic
GGTGTGTTGCAGAATGGGAA+AGG 0.507863 4:-65754604 MsG0480021896.01.T01:intron
AAGCTTAACATACTCTACAT+TGG 0.508834 4:+65754177 None:intergenic
AATGGGAAAGGGTTTGATAT+GGG 0.518936 4:-65754592 MsG0480021896.01.T01:CDS
TTTGATATGGGCGACGGCAG+AGG 0.527398 4:-65754580 MsG0480021896.01.T01:CDS
GTGTGTTGCAGAATGGGAAA+GGG 0.536633 4:-65754603 MsG0480021896.01.T01:intron
GTTCATACTAATTGCGACGA+GGG 0.586529 4:+65754301 None:intergenic
GCGAATGTCAGTGGCAACGA+AGG 0.598642 4:+65754493 None:intergenic
TTTGGGTGGGTTCTGATGAA+CGG 0.599950 4:-65754432 MsG0480021896.01.T01:CDS
CTACATTGGATTCGGCTCGT+AGG 0.624831 4:+65754191 None:intergenic
AAAGGGTTTGATATGGGCGA+CGG 0.633143 4:-65754586 MsG0480021896.01.T01:CDS
AGTCTTGCAGCGAATGTCAG+TGG 0.636620 4:+65754484 None:intergenic
GCGACGGCAGAGGATCTGCA+TGG 0.669341 4:-65754570 MsG0480021896.01.T01:CDS
AAGACTGGCGAAGAAAGCTG+AGG 0.702682 4:-65754467 MsG0480021896.01.T01:CDS
GATCAGGTAATGTGACGAGG+AGG 0.706235 4:+65754338 None:intergenic
AGTTCATACTAATTGCGACG+AGG 0.712101 4:+65754300 None:intergenic
GTTGATCAGGTAATGTGACG+AGG 0.712528 4:+65754335 None:intergenic
ATTGCGACGAGGGAAATGGA+AGG 0.712593 4:+65754311 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAAACAAAAATATAAATAA+AGG - Chr4:65754565-65754584 MsG0480021896.01.T01:CDS 10.0%
!!! TATTTTTATTGTTTTTTACA+TGG + Chr4:65754623-65754642 None:intergenic 10.0%
!! AAATTTCATAGAAAATAAAC+TGG + Chr4:65754878-65754897 None:intergenic 15.0%
!! CACTTTAATTGTATTAAAAA+TGG - Chr4:65754073-65754092 MsG0480021896.01.T01:three_prime_UTR 15.0%
!! GTAAAATCAAATTCTTAATT+GGG - Chr4:65754143-65754162 MsG0480021896.01.T01:three_prime_UTR 15.0%
!! TAAAATCAAATTCTTAATTG+GGG - Chr4:65754144-65754163 MsG0480021896.01.T01:three_prime_UTR 15.0%
!! TGTAAAATCAAATTCTTAAT+TGG - Chr4:65754142-65754161 MsG0480021896.01.T01:three_prime_UTR 15.0%
!! TTATTAGCAAAGAAAAAATA+AGG - Chr4:65754326-65754345 MsG0480021896.01.T01:CDS 15.0%
!!! ACAATAGATCAATAAAATTT+TGG + Chr4:65754664-65754683 None:intergenic 15.0%
!!! AGTTTATTTTCTATGAAATT+TGG - Chr4:65754877-65754896 MsG0480021896.01.T01:intron 15.0%
!!! ATAAAATTTTGGGATAAAAA+TGG + Chr4:65754653-65754672 None:intergenic 15.0%
!!! ATAAATGAAAGTTTAACTTA+AGG - Chr4:65755553-65755572 MsG0480021896.01.T01:CDS 15.0%
!!! CAATAGATCAATAAAATTTT+GGG + Chr4:65754663-65754682 None:intergenic 15.0%
!!! CATTTTTAATACAATTAAAG+TGG + Chr4:65754075-65754094 None:intergenic 15.0%
!!! TTTTATGTTTGTAATTGTTT+GGG + Chr4:65754536-65754555 None:intergenic 15.0%
!!! TTTTTATGTTTGTAATTGTT+TGG + Chr4:65754537-65754556 None:intergenic 15.0%
!! ATCAATTGTGTAATTTAACT+TGG - Chr4:65754223-65754242 MsG0480021896.01.T01:three_prime_UTR 20.0%
!! ATTCTCCAATAAAAATTTCA+CGG - Chr4:65754464-65754483 MsG0480021896.01.T01:CDS 20.0%
!! ATTTCTGTTTAAACTAAGTT+TGG - Chr4:65754996-65755015 MsG0480021896.01.T01:intron 20.0%
!! CCTAATTTCAAATTCTAATT+AGG + Chr4:65755507-65755526 None:intergenic 20.0%
!! GAAAGATACAATTTACTATT+TGG - Chr4:65754404-65754423 MsG0480021896.01.T01:CDS 20.0%
!!! CCTAATTAGAATTTGAAATT+AGG - Chr4:65755504-65755523 MsG0480021896.01.T01:intron 20.0%
!!! TATATGTAAGAAAAAGCATA+CGG - Chr4:65755375-65755394 MsG0480021896.01.T01:intron 20.0%
!!! TCAATTGTGTAATTTAACTT+GGG - Chr4:65754224-65754243 MsG0480021896.01.T01:three_prime_UTR 20.0%
!!! TGCAAATCATGATATTTTTT+TGG - Chr4:65755347-65755366 MsG0480021896.01.T01:intron 20.0%
! AATGCATACATCAAGTTAAT+AGG + Chr4:65754972-65754991 None:intergenic 25.0%
! ATATTAGGTCAAACATAATG+GGG - Chr4:65754252-65754271 MsG0480021896.01.T01:three_prime_UTR 25.0%
! CATATTAGGTCAAACATAAT+GGG - Chr4:65754251-65754270 MsG0480021896.01.T01:three_prime_UTR 25.0%
! CTCATTATAAGCATACAATA+AGG + Chr4:65755477-65755496 None:intergenic 25.0%
! GCTTAAATGCATTTCTTATT+AGG - Chr4:65754914-65754933 MsG0480021896.01.T01:intron 25.0%
! GTTACAAATATACCATTTGA+AGG - Chr4:65754596-65754615 MsG0480021896.01.T01:intron 25.0%
! TCATATTAGGTCAAACATAA+TGG - Chr4:65754250-65754269 MsG0480021896.01.T01:three_prime_UTR 25.0%
!! TTAACTTGGGAATTCATATT+AGG - Chr4:65754237-65754256 MsG0480021896.01.T01:three_prime_UTR 25.0%
AAAGCATACGGTATTCAAAA+TGG - Chr4:65755387-65755406 MsG0480021896.01.T01:intron 30.0%
AACATACTCTACATTGGATT+CGG + Chr4:65755441-65755460 None:intergenic 30.0%
AAGAAATCCCTAATTCTTCT+CGG - Chr4:65754691-65754710 MsG0480021896.01.T01:intron 30.0%
AAGCTTAACATACTCTACAT+TGG + Chr4:65755447-65755466 None:intergenic 30.0%
ACTTGAACAAGAGCAAAAAT+AGG - Chr4:65754108-65754127 MsG0480021896.01.T01:three_prime_UTR 30.0%
AGTATTCAGCAGTATCAATT+AGG + Chr4:65755235-65755254 None:intergenic 30.0%
ATCAGCAATGAAATTGTCAT+GGG - Chr4:65754436-65754455 MsG0480021896.01.T01:CDS 30.0%
CAATAAAAATTTCACGGAAG+AGG - Chr4:65754470-65754489 MsG0480021896.01.T01:CDS 30.0%
CTTGAACAAGAGCAAAAATA+GGG - Chr4:65754109-65754128 MsG0480021896.01.T01:three_prime_UTR 30.0%
TCATAGAAAATAAACTGGAC+AGG + Chr4:65754873-65754892 None:intergenic 30.0%
! CTCTTCCGTGAAATTTTTAT+TGG + Chr4:65754472-65754491 None:intergenic 30.0%
! CTGTTTTGTGAGAAAGAAAA+CGG + Chr4:65754734-65754753 None:intergenic 30.0%
! TCTCTATCTTTTCTCAAGAA+AGG - Chr4:65755254-65755273 MsG0480021896.01.T01:intron 30.0%
! TTTTTACATGGTCCTTCAAA+TGG + Chr4:65754611-65754630 None:intergenic 30.0%
!! TTTTCTATGAAATTTGGAGC+TGG - Chr4:65754883-65754902 MsG0480021896.01.T01:intron 30.0%
AATGGGAAAGGGTTTGATAT+GGG - Chr4:65755029-65755048 MsG0480021896.01.T01:intron 35.0%
CAATTTCATTGCTGATGTGA+CGG + Chr4:65754432-65754451 None:intergenic 35.0%
CATCAGCAATGAAATTGTCA+TGG - Chr4:65754435-65754454 MsG0480021896.01.T01:CDS 35.0%
GGGGAATAGAAAATTGATAG+AGG - Chr4:65754163-65754182 MsG0480021896.01.T01:three_prime_UTR 35.0%
AACTGGACAGGTGAAGAAAT+AGG + Chr4:65754861-65754880 None:intergenic 40.0%
AGTTCATACTAATTGCGACG+AGG + Chr4:65755324-65755343 None:intergenic 40.0%
GAAGAAATAGGAGACAAACC+TGG + Chr4:65754849-65754868 None:intergenic 40.0%
GAATGGGAAAGGGTTTGATA+TGG - Chr4:65755028-65755047 MsG0480021896.01.T01:intron 40.0%
GTTCATACTAATTGCGACGA+GGG + Chr4:65755323-65755342 None:intergenic 40.0%
TTGATGTAACACCGTTACAC+TGG + Chr4:65754503-65754522 None:intergenic 40.0%
! TGAGGTACGTGCAATGTTTT+GGG - Chr4:65755172-65755191 MsG0480021896.01.T01:intron 40.0%
!! AAGTTTGGTGTGTTGCAGAA+TGG - Chr4:65755011-65755030 MsG0480021896.01.T01:intron 40.0%
!! AGTTTGGTGTGTTGCAGAAT+GGG - Chr4:65755012-65755031 MsG0480021896.01.T01:intron 40.0%
AAAGGGTTTGATATGGGCGA+CGG - Chr4:65755035-65755054 MsG0480021896.01.T01:intron 45.0%
AATAGGAGACAAACCTGGAC+AGG + Chr4:65754844-65754863 None:intergenic 45.0%
ACTAATTGCGACGAGGGAAA+TGG + Chr4:65755317-65755336 None:intergenic 45.0%
GAAAGAAAACGGTGTGCAAC+TGG + Chr4:65754723-65754742 None:intergenic 45.0%
GAGGATCCAAACCAGTGTAA+CGG - Chr4:65754489-65754508 MsG0480021896.01.T01:CDS 45.0%
GTGTGTTGCAGAATGGGAAA+GGG - Chr4:65755018-65755037 MsG0480021896.01.T01:intron 45.0%
GTTGATCAGGTAATGTGACG+AGG + Chr4:65755289-65755308 None:intergenic 45.0%
! CTGAGGTACGTGCAATGTTT+TGG - Chr4:65755171-65755190 MsG0480021896.01.T01:intron 45.0%
! GTAACACCGTTACACTGGTT+TGG + Chr4:65754498-65754517 None:intergenic 45.0%
! TTTGGGTGGGTTCTGATGAA+CGG - Chr4:65755189-65755208 MsG0480021896.01.T01:intron 45.0%
!! GGAAATGGAAGGTGTTGATC+AGG + Chr4:65755302-65755321 None:intergenic 45.0%
!! GTACGTGCAATGTTTTGGGT+GGG - Chr4:65755176-65755195 MsG0480021896.01.T01:intron 45.0%
AAAACGGTGTGCAACTGGTC+GGG + Chr4:65754718-65754737 None:intergenic 50.0%
AAGACTGGCGAAGAAAGCTG+AGG - Chr4:65755154-65755173 MsG0480021896.01.T01:intron 50.0%
ACTGACATTCGCTGCAAGAC+TGG - Chr4:65755139-65755158 MsG0480021896.01.T01:intron 50.0%
AGTCTTGCAGCGAATGTCAG+TGG + Chr4:65755140-65755159 None:intergenic 50.0%
ATTGCGACGAGGGAAATGGA+AGG + Chr4:65755313-65755332 None:intergenic 50.0%
GAAAACGGTGTGCAACTGGT+CGG + Chr4:65754719-65754738 None:intergenic 50.0%
GATCAGGTAATGTGACGAGG+AGG + Chr4:65755286-65755305 None:intergenic 50.0%
GCAGAGGATCTGCATGGAAT+AGG - Chr4:65755057-65755076 MsG0480021896.01.T01:intron 50.0%
GGTGTGTTGCAGAATGGGAA+AGG - Chr4:65755017-65755036 MsG0480021896.01.T01:intron 50.0%
GTCGGGACCGAGAAGAATTA+GGG + Chr4:65754701-65754720 None:intergenic 50.0%
! CTACATTGGATTCGGCTCGT+AGG + Chr4:65755433-65755452 None:intergenic 50.0%
!! GGTACGTGCAATGTTTTGGG+TGG - Chr4:65755175-65755194 MsG0480021896.01.T01:intron 50.0%
GCGAATGTCAGTGGCAACGA+AGG + Chr4:65755131-65755150 None:intergenic 55.0%
GGTCGGGACCGAGAAGAATT+AGG + Chr4:65754702-65754721 None:intergenic 55.0%
TTTGATATGGGCGACGGCAG+AGG - Chr4:65755041-65755060 MsG0480021896.01.T01:intron 55.0%
ACAGGTGAACTGCGTGGGTG+TGG + Chr4:65754826-65754845 None:intergenic 60.0%
ACCTGGACAGGTGAACTGCG+TGG + Chr4:65754832-65754851 None:intergenic 60.0%
CCTGGACAGGTGAACTGCGT+GGG + Chr4:65754831-65754850 None:intergenic 60.0%
CCCACGCAGTTCACCTGTCC+AGG - Chr4:65754828-65754847 MsG0480021896.01.T01:intron 65.0%
GCGACGGCAGAGGATCTGCA+TGG - Chr4:65755051-65755070 MsG0480021896.01.T01:intron 65.0%
GGTGAACTGCGTGGGTGTGG+CGG + Chr4:65754823-65754842 None:intergenic 65.0%
GTACGGAGGACCACCGGAGA+GGG + Chr4:65754799-65754818 None:intergenic 65.0%
TACGGAGGACCACCGGAGAG+GGG + Chr4:65754798-65754817 None:intergenic 65.0%
TGCGTGGGTGTGGCGGTGTA+CGG + Chr4:65754816-65754835 None:intergenic 65.0%
TGTACGGAGGACCACCGGAG+AGG + Chr4:65754800-65754819 None:intergenic 65.0%
GGCGGTGTACGGAGGACCAC+CGG + Chr4:65754805-65754824 None:intergenic 70.0%
GTGGGTGTGGCGGTGTACGG+AGG + Chr4:65754813-65754832 None:intergenic 70.0%
AACCCGCCGCCACCCCTCTC+CGG - Chr4:65754783-65754802 MsG0480021896.01.T01:intron 75.0%
GGACCACCGGAGAGGGGTGG+CGG + Chr4:65754792-65754811 None:intergenic 75.0%
GGAGGACCACCGGAGAGGGG+TGG + Chr4:65754795-65754814 None:intergenic 75.0%
!! CACCGGAGAGGGGTGGCGGC+GGG + Chr4:65754788-65754807 None:intergenic 80.0%
!! CCACCGGAGAGGGGTGGCGG+CGG + Chr4:65754789-65754808 None:intergenic 80.0%
! CCGCCGCCACCCCTCTCCGG+TGG - Chr4:65754786-65754805 MsG0480021896.01.T01:intron 85.0%
Chromosome Type Strat End Strand Name
Chr4 gene 65754059 65755584 65754059 ID=MsG0480021896.01;Name=MsG0480021896.01
Chr4 mRNA 65754059 65755584 65754059 ID=MsG0480021896.01.T01;Parent=MsG0480021896.01;Name=MsG0480021896.01.T01;_AED=0.54;_eAED=0.54;_QI=0|0|0.5|0.5|0|0.5|2|244|127
Chr4 exon 65754059 65754614 65754059 ID=MsG0480021896.01.T01:exon:8004;Parent=MsG0480021896.01.T01
Chr4 exon 65755513 65755584 65755513 ID=MsG0480021896.01.T01:exon:8003;Parent=MsG0480021896.01.T01
Chr4 CDS 65755513 65755584 65755513 ID=MsG0480021896.01.T01:cds;Parent=MsG0480021896.01.T01
Chr4 CDS 65754303 65754614 65754303 ID=MsG0480021896.01.T01:cds;Parent=MsG0480021896.01.T01
Chr4 three_prime_UTR 65754059 65754302 65754059 ID=MsG0480021896.01.T01:three_prime_utr;Parent=MsG0480021896.01.T01
Gene Sequence

>MsG0480021896.01.T01

ATGAGTGAATACGCCACTTTAATTGTATTAAAAATGGTTCAATTTCATGACTTGAACAAGAGCAAAAATAGGAATGGGAAAGGGTTTGATATGGGCGACGGCAGAGGATCTGCATGGAATAGGAGTCGAGTTCTGTCGCTATATCGTCACATACTGCGAAGCTTGAATTCTCCTTCGTTGCCACTGACATTCGCTGCAAGACTGGCGAAGAAAGCTGAGGTACGTGCAATGTTTTGGGTGGGTTCTGATGAACGGTCTTTGCATAACATTGCTGACCTAATTGATACTGCTGAATACTCTCTATCTTTTCTCAAGAAAGGTCAATTCCTCCTCGTCACATTACCTGATCAACACCTTCCATTTCCCTCGTCGCAATTAGTATGA

Protein sequence

>MsG0480021896.01.T01

MSEYATLIVLKMVQFHDLNKSKNRNGKGFDMGDGRGSAWNRSRVLSLYRHILRSLNSPSLPLTFAARLAKKAEVRAMFWVGSDERSLHNIADLIDTAEYSLSFLKKGQFLLVTLPDQHLPFPSSQLV*