AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580027923.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027923.01.T01 MTR_5g065350 94.845 194 3 2 1 193 1 188 1.34e-131 367
MsG0580027923.01.T01 MTR_7g445390 45.455 66 34 1 14 79 5 68 4.49e-13 63.5
MsG0580027923.01.T01 MTR_8g005880 43.860 57 32 0 17 73 8 64 2.93e-12 60.8
MsG0580027923.01.T02 MTR_5g065350 96.970 66 1 1 14 78 123 188 1.45e-36 121
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027923.01.T01 AT1G02070 50.943 53 17 2 17 64 5 53 4.59e-11 58.5

Find 39 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGCTTCTGATGGTGATTT+TGG 0.180402 5:-71110223 None:intergenic
TTTGGTTTCTTCATTGGTTT+TGG 0.200009 5:-71110205 None:intergenic
GATGAAGAATTTGAAGAGTT+TGG 0.223033 5:-71110070 None:intergenic
TACTATATATTAAAATAAAA+AGG 0.269571 5:-71110506 None:intergenic
TTGCACGTTAAAATGACATT+AGG 0.278421 5:-71110614 None:intergenic
ACCTGTGTTGAGATCGGATT+TGG 0.307776 5:-71109597 None:intergenic
TTTGTTATGTACATTGCATT+AGG 0.324813 5:-71110549 None:intergenic
CTTATTCCTGAAAACTTGTT+TGG 0.327849 5:-71110389 None:intergenic
ACAACTTAATGAAGTATGTT+TGG 0.352183 5:+71110766 MsG0580027923.01.T01:three_prime_UTR
CGAGTTGAAACTAAAATAGA+TGG 0.362041 5:-71110271 None:intergenic
TGCACGTTAAAATGACATTA+GGG 0.362405 5:-71110613 None:intergenic
CATATGCAACACTCTGTCTT+TGG 0.372972 5:-71109529 None:intergenic
AAAAGTAGTAGTAATAATAT+TGG 0.382026 5:+71110361 MsG0580027923.01.T01:CDS
AATCATCACAAACGGTGCTT+TGG 0.403206 5:+71110300 MsG0580027923.01.T01:CDS
TGTTGCATATGTGGGGATCT+TGG 0.434290 5:+71109541 MsG0580027923.01.T01:CDS
AAGACAGAGTGTTGCATATG+TGG 0.477772 5:+71109532 MsG0580027923.01.T01:CDS
CTTAATGAAGTATGTTTGGA+TGG 0.501678 5:+71110770 MsG0580027923.01.T01:three_prime_UTR
AACTATTAGGACTGAATTGA+CGG 0.501795 5:+71110733 MsG0580027923.01.T01:three_prime_UTR
CGCAAAAGAATCATCACAAA+CGG 0.503705 5:+71110292 MsG0580027923.01.T01:CDS
CAGAAGAAAGGAGTAATCAA+AGG 0.511163 5:+71110157 MsG0580027923.01.T01:CDS
TTAAATACCTGTGTTGAGAT+CGG 0.514874 5:-71109603 None:intergenic
GCAACAAGGAAAATTAGAAT+CGG 0.525386 5:+71110633 MsG0580027923.01.T01:three_prime_UTR
GGAGATGTTGATGATGCTAT+TGG 0.539445 5:-71110250 None:intergenic
AAAGGAGTAATCAAAGGAAG+TGG 0.542816 5:+71110163 MsG0580027923.01.T01:CDS
TTTGGGTGGAAGAGAAGAGA+CGG 0.548215 5:+71110905 MsG0580027923.01.T01:three_prime_UTR
TTGAAGATAGATGAAGAACT+AGG 0.558656 5:-71109412 None:intergenic
AATGAAAGCACAGAAGATGA+AGG 0.559578 5:+71110106 MsG0580027923.01.T01:CDS
AGACAGAGTGTTGCATATGT+GGG 0.559651 5:+71109533 MsG0580027923.01.T01:CDS
AGTCTATATCTTGTTTAACT+CGG 0.560190 5:+71109335 MsG0580027923.01.T01:five_prime_UTR
TTGAGTACTCAATGAGGTTG+TGG 0.572693 5:+71110665 MsG0580027923.01.T01:three_prime_UTR
CAGCAAAACATGACAAGCAA+AGG 0.581246 5:+71109484 MsG0580027923.01.T01:exon
GATAGATGAAGAACTAGGAG+AGG 0.593712 5:-71109407 None:intergenic
GTGCTTTGGAAGAGAAGTTG+AGG 0.598720 5:+71110314 MsG0580027923.01.T01:CDS
ACTTCACAAACACGGTGACA+CGG 0.600656 5:+71109303 None:intergenic
GCCAAATCCGATCTCAACAC+AGG 0.610494 5:+71109596 MsG0580027923.01.T01:CDS
GTACTCAATGAGGTTGTGGT+AGG 0.617424 5:+71110669 MsG0580027923.01.T01:three_prime_UTR
GACAGAGTGTTGCATATGTG+GGG 0.645405 5:+71109534 MsG0580027923.01.T01:CDS
TTGGTGTTTGAGTCAGAACG+AGG 0.661090 5:+71110042 MsG0580027923.01.T01:CDS
TGTTGATTGAGTACTCAATG+AGG 0.683702 5:+71110659 MsG0580027923.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAATTATAAAAAGTTTATTT+GGG + Chr5:71110853-71110872 MsG0580027923.01.T01:three_prime_UTR 10.0%
!! TTCATTAAAAAAAAGATTAA+GGG - Chr5:71109649-71109668 None:intergenic 10.0%
!! TTTCATTAAAAAAAAGATTA+AGG - Chr5:71109650-71109669 None:intergenic 10.0%
!!! TAATATATAGTATTTTGTTT+TGG + Chr5:71110517-71110536 MsG0580027923.01.T01:three_prime_UTR 10.0%
!! AAAAGTAGTAGTAATAATAT+TGG + Chr5:71110361-71110380 MsG0580027923.01.T01:CDS 15.0%
!! AAACAAAAAAAGAAAAGAAA+AGG + Chr5:71110474-71110493 MsG0580027923.01.T01:three_prime_UTR 15.0%
!! AACAAAAAAAGAAAAGAAAA+GGG + Chr5:71110475-71110494 MsG0580027923.01.T01:three_prime_UTR 15.0%
!! CGAATTATAAAAAGTTTATT+TGG + Chr5:71110852-71110871 MsG0580027923.01.T01:three_prime_UTR 15.0%
!!! ATTGTTATAAATATTTTGTG+TGG + Chr5:71110710-71110729 MsG0580027923.01.T01:three_prime_UTR 15.0%
!!! TTTTGTTATTTAATTTGTTG+TGG + Chr5:71109929-71109948 MsG0580027923.01.T01:intron 15.0%
!! TTATAAAAAGTTTATTTGGG+TGG + Chr5:71110891-71110910 MsG0580027923.01.T01:three_prime_UTR 20.0%
!! TTATATATACATCTTTAGTG+AGG + Chr5:71109856-71109875 MsG0580027923.01.T01:intron 20.0%
!!! AAAAATTGTTTTCAGAAGAA+AGG + Chr5:71110145-71110164 MsG0580027923.01.T01:CDS 20.0%
!!! AATTTTTTGGAACATGTAAT+TGG + Chr5:71110023-71110042 MsG0580027923.01.T01:CDS 20.0%
!!! GTATACATATTCAAATTTTG+TGG + Chr5:71110961-71110980 MsG0580027923.01.T01:three_prime_UTR 20.0%
!!! TATTTTTGTAAGGAAATTGA+AGG + Chr5:71109820-71109839 MsG0580027923.01.T01:intron 20.0%
! ACAACTTAATGAAGTATGTT+TGG + Chr5:71110766-71110785 MsG0580027923.01.T01:three_prime_UTR 25.0%
! CTAATGACCACAAATAAAAA+AGG - Chr5:71109387-71109406 None:intergenic 25.0%
!! AGTCTATATCTTGTTTAACT+CGG + Chr5:71109335-71109354 MsG0580027923.01.T01:five_prime_UTR 25.0%
!! ATATTTTGTGTGGAACTATT+AGG + Chr5:71110720-71110739 MsG0580027923.01.T01:three_prime_UTR 25.0%
!! TACCTACAAAATTTTCATGA+AGG - Chr5:71109977-71109996 None:intergenic 25.0%
!! TTTGTTATGTACATTGCATT+AGG - Chr5:71110552-71110571 None:intergenic 25.0%
!!! ATTTAATTTGTTGTGGTCTT+TGG + Chr5:71109936-71109955 MsG0580027923.01.T01:intron 25.0%
!!! CTCTTTACCTTTTTTATTTG+TGG + Chr5:71109377-71109396 MsG0580027923.01.T01:five_prime_UTR 25.0%
!!! TATCCAACAACAGAATTTTT+TGG + Chr5:71110010-71110029 MsG0580027923.01.T01:CDS 25.0%
!!! TGTATCTATTTTTCATTGAG+AGG - Chr5:71109466-71109485 None:intergenic 25.0%
AACTATTAGGACTGAATTGA+CGG + Chr5:71110733-71110752 MsG0580027923.01.T01:three_prime_UTR 30.0%
CTTAATGAAGTATGTTTGGA+TGG + Chr5:71110770-71110789 MsG0580027923.01.T01:three_prime_UTR 30.0%
GATGAAGAATTTGAAGAGTT+TGG - Chr5:71110073-71110092 None:intergenic 30.0%
GCAACAAGGAAAATTAGAAT+CGG + Chr5:71110633-71110652 MsG0580027923.01.T01:three_prime_UTR 30.0%
GTTCCAAAAAATTCTGTTGT+TGG - Chr5:71110016-71110035 None:intergenic 30.0%
TGCACGTTAAAATGACATTA+GGG - Chr5:71110616-71110635 None:intergenic 30.0%
TTAAATACCTGTGTTGAGAT+CGG - Chr5:71109606-71109625 None:intergenic 30.0%
TTGAAGATAGATGAAGAACT+AGG - Chr5:71109415-71109434 None:intergenic 30.0%
TTGCACGTTAAAATGACATT+AGG - Chr5:71110617-71110636 None:intergenic 30.0%
! AGATTTTGCATAGAAAAAGC+TGG - Chr5:71109576-71109595 None:intergenic 30.0%
! CGAGTTGAAACTAAAATAGA+TGG - Chr5:71110274-71110293 None:intergenic 30.0%
! CTCCTTCATGAAAATTTTGT+AGG + Chr5:71109972-71109991 MsG0580027923.01.T01:intron 30.0%
! CTTATTCCTGAAAACTTGTT+TGG - Chr5:71110392-71110411 None:intergenic 30.0%
!! CAAACAAGTTTTCAGGAATA+AGG + Chr5:71110390-71110409 MsG0580027923.01.T01:CDS 30.0%
!! TTTTCAGGAATAAGGTAAGA+AGG + Chr5:71110398-71110417 MsG0580027923.01.T01:CDS 30.0%
!!! GAGCTACATATAGTTTTGAA+AGG + Chr5:71109763-71109782 MsG0580027923.01.T01:intron 30.0%
!!! TTTGGTTTCTTCATTGGTTT+TGG - Chr5:71110208-71110227 None:intergenic 30.0%
AAAGGAGTAATCAAAGGAAG+TGG + Chr5:71110163-71110182 MsG0580027923.01.T01:CDS 35.0%
CAGAAGAAAGGAGTAATCAA+AGG + Chr5:71110157-71110176 MsG0580027923.01.T01:CDS 35.0%
CGCAAAAGAATCATCACAAA+CGG + Chr5:71110292-71110311 MsG0580027923.01.T01:CDS 35.0%
GTTTGGATGGAAGAGAAAAA+AGG + Chr5:71110783-71110802 MsG0580027923.01.T01:three_prime_UTR 35.0%
! AATGAAAGCACAGAAGATGA+AGG + Chr5:71110106-71110125 MsG0580027923.01.T01:CDS 35.0%
! ATTTTTCATTGAGAGGAGAG+AGG - Chr5:71109459-71109478 None:intergenic 35.0%
! TGTTGATTGAGTACTCAATG+AGG + Chr5:71110659-71110678 MsG0580027923.01.T01:three_prime_UTR 35.0%
! TTTTTCATTGAGAGGAGAGA+GGG - Chr5:71109458-71109477 None:intergenic 35.0%
!! ATTGGTGATTTTGCTTCTGA+TGG - Chr5:71110235-71110254 None:intergenic 35.0%
!! TTTGCTTCTGATGGTGATTT+TGG - Chr5:71110226-71110245 None:intergenic 35.0%
!!! ACTCGGTTTAGTCTTTTTTG+TGG + Chr5:71109352-71109371 MsG0580027923.01.T01:five_prime_UTR 35.0%
!!! CTCGGTTTAGTCTTTTTTGT+GGG + Chr5:71109353-71109372 MsG0580027923.01.T01:five_prime_UTR 35.0%
!!! GGTGATTTTGGTTTCTTCAT+TGG - Chr5:71110214-71110233 None:intergenic 35.0%
!!! TGTCATTTTAACGTGCAACA+AGG + Chr5:71110619-71110638 MsG0580027923.01.T01:three_prime_UTR 35.0%
AATCATCACAAACGGTGCTT+TGG + Chr5:71110300-71110319 MsG0580027923.01.T01:CDS 40.0%
CAGCAAAACATGACAAGCAA+AGG + Chr5:71109484-71109503 MsG0580027923.01.T01:exon 40.0%
CATATGCAACACTCTGTCTT+TGG - Chr5:71109532-71109551 None:intergenic 40.0%
GATAGATGAAGAACTAGGAG+AGG - Chr5:71109410-71109429 None:intergenic 40.0%
TTGAGTACTCAATGAGGTTG+TGG + Chr5:71110665-71110684 MsG0580027923.01.T01:three_prime_UTR 40.0%
! GTGTGACCAAACAAGTTTTC+AGG + Chr5:71110383-71110402 MsG0580027923.01.T01:CDS 40.0%
!! AAGACAGAGTGTTGCATATG+TGG + Chr5:71109532-71109551 MsG0580027923.01.T01:CDS 40.0%
!! AGACAGAGTGTTGCATATGT+GGG + Chr5:71109533-71109552 MsG0580027923.01.T01:CDS 40.0%
!! GGAGATGTTGATGATGCTAT+TGG - Chr5:71110253-71110272 None:intergenic 40.0%
ACCTGTGTTGAGATCGGATT+TGG - Chr5:71109600-71109619 None:intergenic 45.0%
GTACTCAATGAGGTTGTGGT+AGG + Chr5:71110669-71110688 MsG0580027923.01.T01:three_prime_UTR 45.0%
TGTTGCATATGTGGGGATCT+TGG + Chr5:71109541-71109560 MsG0580027923.01.T01:CDS 45.0%
TTTGGGTGGAAGAGAAGAGA+CGG + Chr5:71110905-71110924 MsG0580027923.01.T01:three_prime_UTR 45.0%
! TTGGTGTTTGAGTCAGAACG+AGG + Chr5:71110042-71110061 MsG0580027923.01.T01:CDS 45.0%
!! GACAGAGTGTTGCATATGTG+GGG + Chr5:71109534-71109553 MsG0580027923.01.T01:CDS 45.0%
!! GTGCTTTGGAAGAGAAGTTG+AGG + Chr5:71110314-71110333 MsG0580027923.01.T01:CDS 45.0%
!! TACTATATATTAAAATAAAA+AGG - Chr5:71110509-71110528 None:intergenic 5.0%
!!! ATAAATTTTATATTTTTGTA+AGG + Chr5:71109810-71109829 MsG0580027923.01.T01:intron 5.0%
GCCAAATCCGATCTCAACAC+AGG + Chr5:71109596-71109615 MsG0580027923.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr5 gene 71109314 71110991 71109314 ID=MsG0580027923.01;Name=MsG0580027923.01
Chr5 mRNA 71109314 71110991 71109314 ID=MsG0580027923.01.T01;Parent=MsG0580027923.01;Name=MsG0580027923.01.T01;_AED=0.20;_eAED=0.20;_QI=179|1|1|1|0|0|2|541|193
Chr5 exon 71109314 71109617 71109314 ID=MsG0580027923.01.T01:exon:7100;Parent=MsG0580027923.01.T01
Chr5 exon 71109994 71110991 71109994 ID=MsG0580027923.01.T01:exon:7101;Parent=MsG0580027923.01.T01
Chr5 five_prime_UTR 71109314 71109492 71109314 ID=MsG0580027923.01.T01:five_prime_utr;Parent=MsG0580027923.01.T01
Chr5 CDS 71109493 71109617 71109493 ID=MsG0580027923.01.T01:cds;Parent=MsG0580027923.01.T01
Chr5 CDS 71109994 71110450 71109994 ID=MsG0580027923.01.T01:cds;Parent=MsG0580027923.01.T01
Chr5 three_prime_UTR 71110451 71110991 71110451 ID=MsG0580027923.01.T01:three_prime_utr;Parent=MsG0580027923.01.T01
Chr5 mRNA 71110214 71110450 71110214 ID=MsG0580027923.01.T02;Parent=MsG0580027923.01;Name=MsG0580027923.01.T02;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|0|1|0|78
Chr5 exon 71110214 71110450 71110214 ID=MsG0580027923.01.T02:exon:7102;Parent=MsG0580027923.01.T02
Chr5 CDS 71110214 71110450 71110214 ID=MsG0580027923.01.T02:cds;Parent=MsG0580027923.01.T02
Gene Sequence

>MsG0580027923.01.T01

ATGACAAGCAAAGGAACAAACTCTGCAGAACAACTACCAAAGACAGAGTGTTGCATATGTGGGGATCTTGGTTTCTCTGACCAGCTTTTTCTATGCAAAATCTGCCAAATCCGATCTCAACACAGGTATTGTAGTAACATATATCCAACAACAGAATTTTTTGGAACATGTAATTGGTGTTTGAGTCAGAACGAGGATTCACCAAACTCTTCAAATTCTTCATCTTCTTACAAGAAAAATGAAAGCACAGAAGATGAAGGAAAGAACAAACAAATAAAAAATTGTTTTCAGAAGAAAGGAGTAATCAAAGGAAGTGGTGTTTATCGTCTTCAACTTCCAAAACCAATGAAGAAACCAAAATCACCATCAGAAGCAAAATCACCAATAGCATCATCAACATCTCCATCTATTTTAGTTTCAACTCGCAAAAGAATCATCACAAACGGTGCTTTGGAAGAGAAGTTGAGGAGAACAAGATCAGAAAACATTATAAAAAGTAGTAGTAATAATATTGGTGTGACCAAACAAGTTTTCAGGAATAAGGTAAGAAGGTATAAGCTTTTAGATGAAGTTTCAAGTTAA

>MsG0580027923.01.T02

ATGAAGAAACCAAAATCACCATCAGAAGCAAAATCACCAATAGCATCATCAACATCTCCATCTATTTTAGTTTCAACTCGCAAAAGAATCATCACAAACGGTGCTTTGGAAGAGAAGTTGAGGAGAACAAGATCAGAAAACATTATAAAAAGTAGTAGTAATAATATTGGTGTGACCAAACAAGTTTTCAGGAATAAGGTAAGAAGGTATAAGCTTTTAGATGAAGTTTCAAGTTAA

Protein sequence

>MsG0580027923.01.T01

MTSKGTNSAEQLPKTECCICGDLGFSDQLFLCKICQIRSQHRYCSNIYPTTEFFGTCNWCLSQNEDSPNSSNSSSSYKKNESTEDEGKNKQIKNCFQKKGVIKGSGVYRLQLPKPMKKPKSPSEAKSPIASSTSPSILVSTRKRIITNGALEEKLRRTRSENIIKSSSNNIGVTKQVFRNKVRRYKLLDEVSS*

>MsG0580027923.01.T02

MKKPKSPSEAKSPIASSTSPSILVSTRKRIITNGALEEKLRRTRSENIIKSSSNNIGVTKQVFRNKVRRYKLLDEVSS*