AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022392.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022392.01.T01 MTR_4g088850 97.333 75 2 0 1 75 1 75 1.29e-49 153
MsG0480022392.01.T01 MTR_4g088850 97.333 75 2 0 1 75 1 75 9.33e-49 150
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022392.01.T01 AT4G21610 57.746 71 20 3 15 76 25 94 1.26e-17 72.8
MsG0480022392.01.T01 AT4G21610 57.746 71 20 3 15 76 25 94 2.39e-17 71.6

Find 31 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTCGTCTTCTTTGTTTACT+AGG 0.247921 4:-72006577 None:intergenic
GGCCGTGGGCGTTGCGGTGG+GGG 0.301669 4:-72006629 None:intergenic
TGGCCGTGGGCGTTGCGGTG+GGG 0.306898 4:-72006630 None:intergenic
GGTGGCCGTGGGCGTTGCGG+TGG 0.333387 4:-72006632 None:intergenic
AGTGACGGTGGCGGTGGCCG+TGG 0.335089 4:-72006644 None:intergenic
GCATGAACATTTGACATGTT+TGG 0.343047 4:-72006978 None:intergenic
GTGGCCGTGGGCGTTGCGGT+GGG 0.370495 4:-72006631 None:intergenic
CTTCTTTGTTTACTAGGTGT+TGG 0.400108 4:-72006571 None:intergenic
CTAATACAATGTTGACTGTC+TGG 0.430906 4:-72007003 None:intergenic
GTGACGGTGGCGGTGGCCGT+GGG 0.451108 4:-72006643 None:intergenic
GATGGGAGTGACGGTGGCGG+TGG 0.475419 4:-72006650 None:intergenic
ATAGGTTGCCAACCAGGTGG+CGG 0.493264 4:-72006605 None:intergenic
TGTAGTGTGTGAAGAAGTAA+CGG 0.499517 4:+72006519 None:intergenic
GGCGGTGGCCGTGGGCGTTG+CGG 0.501640 4:-72006635 None:intergenic
ACAGTCAACATTGTATTAGA+AGG 0.509196 4:+72007007 MsG0480022392.01.T01:CDS
TAGGTTGCCAACCAGGTGGC+GGG 0.511813 4:-72006604 None:intergenic
CATTTGACATGTTTGGCACC+TGG 0.517148 4:-72006971 None:intergenic
CGCCTGCTTTCGTATCCACC+AGG 0.522323 4:+72006953 MsG0480022392.01.T01:CDS
CACCTGGTGGATACGAAAGC+AGG 0.543193 4:-72006955 None:intergenic
CCGGATGGGAGTGACGGTGG+CGG 0.550715 4:-72006653 None:intergenic
ACGACGGCCCGCCACCTGGT+TGG 0.559468 4:+72006597 MsG0480022392.01.T01:CDS
CCGCCACCGTCACTCCCATC+CGG 0.560200 4:+72006653 MsG0480022392.01.T01:CDS
GACGACGACGGCCCGCCACC+TGG 0.561082 4:+72006593 MsG0480022392.01.T01:CDS
GTAGTGTGTGAAGAAGTAAC+GGG 0.584958 4:+72006520 MsG0480022392.01.T01:five_prime_UTR
AACTGACCGGATGGGAGTGA+CGG 0.591272 4:-72006659 None:intergenic
TAAATAGCAACTGACCGGAT+GGG 0.614535 4:-72006667 None:intergenic
TGACCGGATGGGAGTGACGG+TGG 0.636305 4:-72006656 None:intergenic
TTGACATGTTTGGCACCTGG+TGG 0.642814 4:-72006968 None:intergenic
GGAATAGGTTGCCAACCAGG+TGG 0.649831 4:-72006608 None:intergenic
CTGGTGGATACGAAAGCAGG+CGG 0.684251 4:-72006952 None:intergenic
GTAAACAAAGAAGACGACGA+CGG 0.734106 4:+72006581 MsG0480022392.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CCATAAAAGGTTAAAACTAA+AGG - Chr4:72006844-72006863 None:intergenic 25.0%
! GAGAAAAAAAGAGAAGTAAT+AGG - Chr4:72006714-72006733 None:intergenic 25.0%
!!! CCTTTAGTTTTAACCTTTTA+TGG + Chr4:72006841-72006860 MsG0480022392.01.T01:intron 25.0%
!!! TCAAATGAATTGGTTTTTTG+TGG + Chr4:72006865-72006884 MsG0480022392.01.T01:intron 25.0%
AACAAAAGCTGTGGAAAAAA+GGG + Chr4:72006779-72006798 MsG0480022392.01.T01:intron 30.0%
ACAGTCAACATTGTATTAGA+AGG + Chr4:72007007-72007026 MsG0480022392.01.T01:CDS 30.0%
AGACATGTTCATGAGAAAAA+AGG - Chr4:72007041-72007060 None:intergenic 30.0%
ATGAATAAATAGCAACTGAC+CGG - Chr4:72006675-72006694 None:intergenic 30.0%
CAATTCATTTGAGCCATAAA+AGG - Chr4:72006857-72006876 None:intergenic 30.0%
! CTTTTATGGCTCAAATGAAT+TGG + Chr4:72006855-72006874 MsG0480022392.01.T01:intron 30.0%
!! AAGCTCGTAGCTTTAATATT+TGG + Chr4:72006734-72006753 MsG0480022392.01.T01:intron 30.0%
!! AGCTCGTAGCTTTAATATTT+GGG + Chr4:72006735-72006754 MsG0480022392.01.T01:intron 30.0%
!! GGTTCAATTATTTCTGAAAG+AGG + Chr4:72006756-72006775 MsG0480022392.01.T01:intron 30.0%
ACAAAAGCTGTGGAAAAAAG+GGG + Chr4:72006780-72006799 MsG0480022392.01.T01:intron 35.0%
GAACAAAAGCTGTGGAAAAA+AGG + Chr4:72006778-72006797 MsG0480022392.01.T01:intron 35.0%
GCATGAACATTTGACATGTT+TGG - Chr4:72006981-72007000 None:intergenic 35.0%
TTTGATACAGTTGTGTGAAC+AGG + Chr4:72006899-72006918 MsG0480022392.01.T01:intron 35.0%
! CTAATACAATGTTGACTGTC+TGG - Chr4:72007006-72007025 None:intergenic 35.0%
! CTTCTTTGTTTACTAGGTGT+TGG - Chr4:72006574-72006593 None:intergenic 35.0%
ATAAATAGCAACTGACCGGA+TGG - Chr4:72006671-72006690 None:intergenic 40.0%
GTAAACAAAGAAGACGACGA+CGG + Chr4:72006581-72006600 MsG0480022392.01.T01:CDS 40.0%
GTAGTGTGTGAAGAAGTAAC+GGG + Chr4:72006520-72006539 MsG0480022392.01.T01:five_prime_UTR 40.0%
TAAAGGAAGCTTCAAGCTTC+AGG - Chr4:72006827-72006846 None:intergenic 40.0%
TAAATAGCAACTGACCGGAT+GGG - Chr4:72006670-72006689 None:intergenic 40.0%
TGAAAGAGGAACAAAAGCTG+TGG + Chr4:72006770-72006789 MsG0480022392.01.T01:intron 40.0%
! CGTCGTCTTCTTTGTTTACT+AGG - Chr4:72006580-72006599 None:intergenic 40.0%
! GGTTTTGCTCAAATGGTTTG+TGG + Chr4:72006920-72006939 MsG0480022392.01.T01:intron 40.0%
! GTGAACAGGTTTTGCTCAAA+TGG + Chr4:72006913-72006932 MsG0480022392.01.T01:intron 40.0%
AGCTGTGGAAAAAAGGGGTT+TGG + Chr4:72006785-72006804 MsG0480022392.01.T01:intron 45.0%
CATTTGACATGTTTGGCACC+TGG - Chr4:72006974-72006993 None:intergenic 45.0%
TTGACATGTTTGGCACCTGG+TGG - Chr4:72006971-72006990 None:intergenic 50.0%
AACTGACCGGATGGGAGTGA+CGG - Chr4:72006662-72006681 None:intergenic 55.0%
ATAGGTTGCCAACCAGGTGG+CGG - Chr4:72006608-72006627 None:intergenic 55.0%
GGAATAGGTTGCCAACCAGG+TGG - Chr4:72006611-72006630 None:intergenic 55.0%
! CACCTGGTGGATACGAAAGC+AGG - Chr4:72006958-72006977 None:intergenic 55.0%
! CTGGTGGATACGAAAGCAGG+CGG - Chr4:72006955-72006974 None:intergenic 55.0%
CGCCTGCTTTCGTATCCACC+AGG + Chr4:72006953-72006972 MsG0480022392.01.T01:CDS 60.0%
GGGGGAATAGGTTGCCAACC+AGG - Chr4:72006614-72006633 None:intergenic 60.0%
TAGGTTGCCAACCAGGTGGC+GGG - Chr4:72006607-72006626 None:intergenic 60.0%
TGACCGGATGGGAGTGACGG+TGG - Chr4:72006659-72006678 None:intergenic 65.0%
CCGCCACCGTCACTCCCATC+CGG + Chr4:72006653-72006672 MsG0480022392.01.T01:CDS 70.0%
CCGGATGGGAGTGACGGTGG+CGG - Chr4:72006656-72006675 None:intergenic 70.0%
TTCCCCCACCGCAACGCCCA+CGG + Chr4:72006627-72006646 MsG0480022392.01.T01:CDS 70.0%
! GATGGGAGTGACGGTGGCGG+TGG - Chr4:72006653-72006672 None:intergenic 70.0%
! GGGCGTTGCGGTGGGGGAAT+AGG - Chr4:72006626-72006645 None:intergenic 70.0%
ACGACGGCCCGCCACCTGGT+TGG + Chr4:72006597-72006616 MsG0480022392.01.T01:CDS 75.0%
! AGTGACGGTGGCGGTGGCCG+TGG - Chr4:72006647-72006666 None:intergenic 75.0%
! GTGACGGTGGCGGTGGCCGT+GGG - Chr4:72006646-72006665 None:intergenic 75.0%
! GTGGCCGTGGGCGTTGCGGT+GGG - Chr4:72006634-72006653 None:intergenic 75.0%
! TGGCCGTGGGCGTTGCGGTG+GGG - Chr4:72006633-72006652 None:intergenic 75.0%
! GACGACGACGGCCCGCCACC+TGG + Chr4:72006593-72006612 MsG0480022392.01.T01:CDS 80.0%
!! GGCCGTGGGCGTTGCGGTGG+GGG - Chr4:72006632-72006651 None:intergenic 80.0%
!! GGCGGTGGCCGTGGGCGTTG+CGG - Chr4:72006638-72006657 None:intergenic 80.0%
!! GGTGGCCGTGGGCGTTGCGG+TGG - Chr4:72006635-72006654 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr4 gene 72006520 72007069 72006520 ID=MsG0480022392.01;Name=MsG0480022392.01
Chr4 mRNA 72006520 72007069 72006520 ID=MsG0480022392.01.T01;Parent=MsG0480022392.01;Name=MsG0480022392.01.T01;_AED=0.50;_eAED=0.56;_QI=40|1|0.5|1|1|1|2|0|87
Chr4 exon 72006520 72006674 72006520 ID=MsG0480022392.01.T01:exon:4928;Parent=MsG0480022392.01.T01
Chr4 exon 72006921 72007069 72006921 ID=MsG0480022392.01.T01:exon:4929;Parent=MsG0480022392.01.T01
Chr4 five_prime_UTR 72006520 72006559 72006520 ID=MsG0480022392.01.T01:five_prime_utr;Parent=MsG0480022392.01.T01
Chr4 CDS 72006560 72006674 72006560 ID=MsG0480022392.01.T01:cds;Parent=MsG0480022392.01.T01
Chr4 CDS 72006921 72007069 72006921 ID=MsG0480022392.01.T01:cds;Parent=MsG0480022392.01.T01
Gene Sequence

>MsG0480022392.01.T01

ATGATGCAAAGCCAACACCTAGTAAACAAAGAAGACGACGACGGCCCGCCACCTGGTTGGCAACCTATTCCCCCACCGCAACGCCCACGGCCACCGCCACCGTCACTCCCATCCGGTTTTGCTCAAATGGTTTGTGGTTCTTGTCGCCGCCTGCTTTCGTATCCACCAGGTGCCAAACATGTCAAATGTTCATGCTGCCAGACAGTCAACATTGTATTAGAAGGTCAGTTCTCCTTTTTTCTCATGAACATGTCTATGCTGTGA

Protein sequence

>MsG0480022392.01.T01

MMQSQHLVNKEDDDGPPPGWQPIPPPQRPRPPPPSLPSGFAQMVCGSCRRLLSYPPGAKHVKCSCCQTVNIVLEGQFSFFLMNMSML*