AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000899.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000899.01.T01 MTR_3g008960 71.698 265 22 1 1 212 1 265 3.45e-132 380
MsG0180000899.01.T01 MTR_3g009010 71.910 267 20 2 1 212 72 338 1.69e-131 381
MsG0180000899.01.T01 MTR_3g009020 61.885 244 36 3 26 212 6 249 7.99e-98 293
MsG0180000899.01.T01 MTR_3g009030 60.806 273 46 5 1 212 1 273 8.16e-97 290
MsG0180000899.01.T01 MTR_8g091310 47.826 276 75 5 6 212 6 281 1.15e-79 247
MsG0180000899.01.T01 MTR_5g062190 45.956 272 87 5 1 212 6 277 6.03e-75 234
MsG0180000899.01.T01 MTR_5g062290 45.802 262 85 5 8 212 14 275 6.32e-72 227
MsG0180000899.01.T01 MTR_3g086750 44.776 268 83 9 9 212 9 275 2.99e-64 207
MsG0180000899.01.T01 MTR_5g061980 52.459 122 50 4 95 209 72 192 1.10e-35 128
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 77 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACATGATCACATAAGTATT+TGG 0.123305 1:+12932681 None:intergenic
CTATTATTTGAGCTTCTTAT+TGG 0.156319 1:-12936508 MsG0180000899.01.T01:CDS
ACATATCTTTGCGCTGAAAA+TGG 0.170576 1:-12934772 MsG0180000899.01.T01:CDS
GCTTACAAAGCTGTGAATTT+AGG 0.195268 1:-12936450 MsG0180000899.01.T01:CDS
TGCATTACTACAATATGTTT+TGG 0.227561 1:-12932587 MsG0180000899.01.T01:three_prime_UTR
CTTCTGGTTGGGAAACATTC+AGG 0.235880 1:-12934714 MsG0180000899.01.T01:CDS
TTCAGATTTATGTCACAAAA+TGG 0.249547 1:-12933658 MsG0180000899.01.T01:CDS
CTCAAGTGGTTGATCGAAAA+TGG 0.301104 1:-12931854 MsG0180000899.01.T01:three_prime_UTR
CAGATTATGAGGGCACAAAT+TGG 0.303729 1:-12933907 MsG0180000899.01.T01:CDS
TTTGTGCTTGGGCATATCTT+TGG 0.317830 1:+12931948 None:intergenic
ATGATTATACTGACATTTGT+AGG 0.331325 1:+12931883 None:intergenic
ACATGATCACATAAGTATTT+GGG 0.338303 1:+12932682 None:intergenic
CATATCTTTGCGCTGAAAAT+GGG 0.349908 1:-12934771 MsG0180000899.01.T01:CDS
ACAAAAGGTTTAGGTGGGTT+TGG 0.353332 1:+12933589 None:intergenic
AAGCTGTGAATTTAGGAAAC+TGG 0.354101 1:-12936443 MsG0180000899.01.T01:CDS
ATTGAACCTTCACACATATA+TGG 0.363996 1:+12933948 None:intergenic
GCCGACCGTAGTTCACCTTC+TGG 0.397256 1:-12934730 MsG0180000899.01.T01:CDS
ATCAGTGACTGCAGATTATG+AGG 0.405023 1:-12933918 MsG0180000899.01.T01:CDS
CTATCTTGATTGACACTTAG+TGG 0.410417 1:+12932753 None:intergenic
GTGTATTCTCATGCAACATT+TGG 0.419146 1:-12931920 MsG0180000899.01.T01:three_prime_UTR
CCGTAGTTCACCTTCTGGTT+GGG 0.427471 1:-12934725 MsG0180000899.01.T01:CDS
TTCTTCTCTCATTTGTCAAA+GGG 0.428332 1:-12932632 MsG0180000899.01.T01:three_prime_UTR
AAGATATGCCCAAGCACAAA+TGG 0.440605 1:-12931945 MsG0180000899.01.T01:three_prime_UTR
ATGGAACCTTCTCGCTTTGA+CGG 0.442568 1:-12936402 MsG0180000899.01.T01:CDS
CGCCATAACCGTTAGTAAGT+TGG 0.445302 1:+12933825 None:intergenic
TACCAACTTACTAACGGTTA+TGG 0.446796 1:-12933827 MsG0180000899.01.T01:CDS
CTTCTTCTCTCATTTGTCAA+AGG 0.449835 1:-12932633 MsG0180000899.01.T01:three_prime_UTR
ACAAATGTCAGTATAATCAT+TGG 0.459731 1:-12931880 MsG0180000899.01.T01:three_prime_UTR
ACCGTAGTTCACCTTCTGGT+TGG 0.463053 1:-12934726 MsG0180000899.01.T01:CDS
TTTCAGCGCAAAGATATGTC+TGG 0.466042 1:+12934776 None:intergenic
ATACACATCCATTTGTGCTT+GGG 0.467668 1:+12931937 None:intergenic
TAGGGGAATGGACGGTTGAA+TGG 0.472771 1:-12932000 MsG0180000899.01.T01:three_prime_UTR
TTCTGGTTGGGAAACATTCA+GGG 0.477116 1:-12934713 MsG0180000899.01.T01:intron
AGATTATGAGGGCACAAATT+GGG 0.477446 1:-12933906 MsG0180000899.01.T01:CDS
TGAGCTATCCACCATCCAAC+AGG 0.483400 1:+12933616 None:intergenic
GACAGAGCCGGGGATCTTAG+TGG 0.484890 1:-12932513 MsG0180000899.01.T01:three_prime_UTR
AATACACATCCATTTGTGCT+TGG 0.485189 1:+12931936 None:intergenic
TTCTCATGCAACATTTGGAT+GGG 0.487793 1:-12931915 MsG0180000899.01.T01:three_prime_UTR
AATTGTCAACAAAGATCTCT+TGG 0.496005 1:-12936379 MsG0180000899.01.T01:intron
ACATAAGAAATGACAGAGCC+GGG 0.500757 1:-12932524 MsG0180000899.01.T01:three_prime_UTR
CACTTGACAATGCTTTCACA+TGG 0.508127 1:-12933549 MsG0180000899.01.T01:CDS
ATTCTCATGCAACATTTGGA+TGG 0.518902 1:-12931916 MsG0180000899.01.T01:three_prime_UTR
ACTGGCTTGTTGCTGAAGGA+TGG 0.521485 1:-12936425 MsG0180000899.01.T01:CDS
TGTGATCATGTCAAATCCAT+TGG 0.526301 1:-12932670 MsG0180000899.01.T01:three_prime_UTR
CTGCCTTGTAATAGTTCTTG+AGG 0.537261 1:+12932730 None:intergenic
TACATAAGAAATGACAGAGC+CGG 0.537623 1:-12932525 MsG0180000899.01.T01:three_prime_UTR
CCCCAACAAAAGGTTTAGGT+GGG 0.545640 1:+12933584 None:intergenic
GCTTGTTGCTGAAGGATGGA+TGG 0.547369 1:-12936421 MsG0180000899.01.T01:CDS
ACGGTTGAATGGAGCATACA+AGG 0.549974 1:-12931989 MsG0180000899.01.T01:three_prime_UTR
ATATCTTTGCGCTGAAAATG+GGG 0.554384 1:-12934770 MsG0180000899.01.T01:CDS
TTTCTTACCCTCATGACTTG+AGG 0.556009 1:+12933794 None:intergenic
CAGTTATGTATGAATTCCAA+TGG 0.561323 1:+12933689 None:intergenic
GAGAAGAAGTTTAGAATCTG+TGG 0.563821 1:+12932647 None:intergenic
AGTTTAGAATCTGTGGCCAA+TGG 0.566970 1:+12932654 None:intergenic
GGAAACTGGCTTGTTGCTGA+AGG 0.571457 1:-12936429 MsG0180000899.01.T01:CDS
ACAATTGTAAGCACATTACA+AGG 0.581323 1:-12933854 MsG0180000899.01.T01:CDS
CCTTGACCATATATGTGTGA+AGG 0.600036 1:-12933954 MsG0180000899.01.T01:CDS
ACCAGAAGGTGAACTACGGT+CGG 0.604107 1:+12934729 None:intergenic
ACAATTCCGTCAAAGCGAGA+AGG 0.606902 1:+12936396 None:intergenic
TATTGTAGTAATGCACATCA+AGG 0.609097 1:+12932595 None:intergenic
ATAGAGCTCCTCAAGTCATG+AGG 0.611152 1:-12933802 MsG0180000899.01.T01:CDS
CTGAATGTTTCCCAACCAGA+AGG 0.618940 1:+12934715 None:intergenic
TAGCCTCAAGAACTATTACA+AGG 0.620823 1:-12932733 MsG0180000899.01.T01:three_prime_UTR
CCTTCACACATATATGGTCA+AGG 0.624691 1:+12933954 None:intergenic
CCCAACCAGAAGGTGAACTA+CGG 0.625802 1:+12934725 None:intergenic
ATAATCATTGGAGTCTCAAG+TGG 0.635772 1:-12931868 MsG0180000899.01.T01:three_prime_UTR
TGAGAATACCTGTTGGATGG+TGG 0.641335 1:-12933624 MsG0180000899.01.T01:CDS
CAGTGAGAATACCTGTTGGA+TGG 0.643788 1:-12933627 MsG0180000899.01.T01:CDS
TCAGTGACTGCAGATTATGA+GGG 0.647319 1:-12933917 MsG0180000899.01.T01:CDS
TAAGCAAACGAAAGAGAGTG+AGG 0.651768 1:+12936468 None:intergenic
GGAGAATACCAACTTACTAA+CGG 0.652885 1:-12933833 MsG0180000899.01.T01:CDS
AGGCAGCTTATGATGTTGTG+AGG 0.665952 1:-12932713 MsG0180000899.01.T01:three_prime_UTR
TAGAGCTCCTCAAGTCATGA+GGG 0.665978 1:-12933801 MsG0180000899.01.T01:intron
TATCTTTGCGCTGAAAATGG+GGG 0.665981 1:-12934769 MsG0180000899.01.T01:CDS
ACTTGACAATGCTTTCACAT+GGG 0.683824 1:-12933548 MsG0180000899.01.T01:CDS
GATGCAGTGAGAATACCTGT+TGG 0.688209 1:-12933631 MsG0180000899.01.T01:CDS
CATAAGAAATGACAGAGCCG+GGG 0.716341 1:-12932523 MsG0180000899.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATAAATTAATAT+GGG + Chr1:12932014-12932033 None:intergenic 0.0%
!! TATATATATATAAATTAATA+TGG + Chr1:12932015-12932034 None:intergenic 0.0%
!! AGAGATTATATTATATATTT+AGG - Chr1:12936306-12936325 MsG0180000899.01.T01:intron 10.0%
!! TCAAATATCAAAATTTAATA+TGG + Chr1:12933520-12933539 None:intergenic 10.0%
!! TTTGTTAAATATTATAAAGA+CGG + Chr1:12932335-12932354 None:intergenic 10.0%
!!! ATTACATGATTTTATTAAAA+TGG + Chr1:12934018-12934037 None:intergenic 10.0%
!! AATAGACTATAAAAAATATG+GGG + Chr1:12933302-12933321 None:intergenic 15.0%
!! AATCATAAATTTACAAAGAA+AGG + Chr1:12935398-12935417 None:intergenic 15.0%
!! CAATAGACTATAAAAAATAT+GGG + Chr1:12933303-12933322 None:intergenic 15.0%
!! TATAGTAATAGAAAAAGTTT+AGG - Chr1:12935562-12935581 MsG0180000899.01.T01:intron 15.0%
!!! AAAAGTTAAGTTCTAATTAT+TGG - Chr1:12933746-12933765 MsG0180000899.01.T01:intron 15.0%
!!! ATAATTTTAAAATGACATGA+TGG - Chr1:12935246-12935265 MsG0180000899.01.T01:intron 15.0%
!!! ATATAATATAATAGTTTTGG+TGG + Chr1:12932155-12932174 None:intergenic 15.0%
!!! ATGATTTTACTTTTCTATTT+TGG - Chr1:12936337-12936356 MsG0180000899.01.T01:intron 15.0%
!!! CAATTTTTTTTTACTGTTTA+TGG + Chr1:12935181-12935200 None:intergenic 15.0%
!!! CATTTTGAAACAAAAATTTT+TGG + Chr1:12932927-12932946 None:intergenic 15.0%
!!! CTTTCTTTTAAAAGTTTATA+AGG + Chr1:12933263-12933282 None:intergenic 15.0%
!!! TGTTTTAATTTAGATTGTAA+AGG - Chr1:12933172-12933191 MsG0180000899.01.T01:intron 15.0%
!!! TTTTGATATTTGATGATTTT+AGG - Chr1:12933527-12933546 MsG0180000899.01.T01:intron 15.0%
!!! TTTTTTTGTCAATAGTTTAA+TGG + Chr1:12932620-12932639 None:intergenic 15.0%
!! AAAAGTTTGAGTATTTATTG+AGG + Chr1:12933002-12933021 None:intergenic 20.0%
!! AAAGTTTGAGTATTTATTGA+GGG + Chr1:12933001-12933020 None:intergenic 20.0%
!! AAATATATACGTACTAGAAA+AGG - Chr1:12932364-12932383 MsG0180000899.01.T01:intron 20.0%
!! AAGAAACATTTCAGATTATA+AGG - Chr1:12933898-12933917 MsG0180000899.01.T01:CDS 20.0%
!! AGATATGTAAAGTAATATAG+AGG + Chr1:12935460-12935479 None:intergenic 20.0%
!! ATAGACTATAAAAAATATGG+GGG + Chr1:12933301-12933320 None:intergenic 20.0%
!! CCAATAGACTATAAAAAATA+TGG + Chr1:12933304-12933323 None:intergenic 20.0%
!! GAAAAAATCATATATACCAA+AGG + Chr1:12932187-12932206 None:intergenic 20.0%
!! GAACAAAATTAAAGTTGTAT+TGG + Chr1:12932967-12932986 None:intergenic 20.0%
!! GTAACTTGATTTAAAAGAAT+TGG + Chr1:12934079-12934098 None:intergenic 20.0%
!! TTTGTAATGCTTGATAAAAT+TGG - Chr1:12932813-12932832 MsG0180000899.01.T01:intron 20.0%
!!! AAAAAATGTTAAAGTACTCA+AGG - Chr1:12932469-12932488 MsG0180000899.01.T01:intron 20.0%
!!! AAAAATGTTAAAGTACTCAA+GGG - Chr1:12932470-12932489 MsG0180000899.01.T01:intron 20.0%
!!! CCATATTTTTTATAGTCTAT+TGG - Chr1:12933301-12933320 MsG0180000899.01.T01:intron 20.0%
!!! GTTTAATTTTAAGCCAAATA+TGG - Chr1:12932709-12932728 MsG0180000899.01.T01:three_prime_UTR 20.0%
!!! TACATTGTTTGATTTAAAAC+CGG + Chr1:12933400-12933419 None:intergenic 20.0%
!!! TTATAATCTGAAATGTTTCT+TGG + Chr1:12933899-12933918 None:intergenic 20.0%
!!! TTTAAGTTTGTCTTTTTCTT+GGG - Chr1:12933046-12933065 MsG0180000899.01.T01:intron 20.0%
!!! TTTACTTTTCTATTTTGGTA+GGG - Chr1:12936342-12936361 MsG0180000899.01.T01:intron 20.0%
!!! TTTTACTTTTCTATTTTGGT+AGG - Chr1:12936341-12936360 MsG0180000899.01.T01:intron 20.0%
!!! TTTTTGTGAACTAAGTTTTA+AGG + Chr1:12935486-12935505 None:intergenic 20.0%
! AAAGAAAGGTAAATGTTGAT+AGG + Chr1:12935384-12935403 None:intergenic 25.0%
! AAGAAAGGTAAATGTTGATA+GGG + Chr1:12935383-12935402 None:intergenic 25.0%
! AATAATGAACTTGCAAGATT+TGG - Chr1:12934610-12934629 MsG0180000899.01.T01:intron 25.0%
! ACAAATGTCAGTATAATCAT+TGG - Chr1:12936480-12936499 MsG0180000899.01.T01:CDS 25.0%
! ACATGATCACATAAGTATTT+GGG + Chr1:12935681-12935700 None:intergenic 25.0%
! AGTAATAGAAAAAGTTTAGG+AGG - Chr1:12935565-12935584 MsG0180000899.01.T01:intron 25.0%
! ATATATAGACCAACTAACAT+TGG + Chr1:12935925-12935944 None:intergenic 25.0%
! ATGATTATACTGACATTTGT+AGG + Chr1:12936480-12936499 None:intergenic 25.0%
! ATTATATTATGAGTCTCCTT+TGG - Chr1:12932168-12932187 MsG0180000899.01.T01:intron 25.0%
! ATTGTCATATGAAAAACTGA+TGG + Chr1:12934491-12934510 None:intergenic 25.0%
! CTATTATTTGAGCTTCTTAT+TGG - Chr1:12931852-12931871 MsG0180000899.01.T01:three_prime_UTR 25.0%
! GAAATGAATTCTACATAAAG+TGG - Chr1:12934197-12934216 MsG0180000899.01.T01:intron 25.0%
! GGTAAGTAATGTATATATCT+AGG - Chr1:12933668-12933687 MsG0180000899.01.T01:CDS 25.0%
! GTGTTCAACAAAAAATTTGT+GGG - Chr1:12933816-12933835 MsG0180000899.01.T01:CDS 25.0%
! TAGACAATGATGAATAAACA+AGG + Chr1:12932689-12932708 None:intergenic 25.0%
! TATTTATTGAGGGTAAAGTT+GGG + Chr1:12932991-12933010 None:intergenic 25.0%
! TGTTCAACAAAAAATTTGTG+GGG - Chr1:12933817-12933836 MsG0180000899.01.T01:CDS 25.0%
! TTCAGATTTATGTCACAAAA+TGG - Chr1:12934702-12934721 MsG0180000899.01.T01:intron 25.0%
! TTTGTGGGGTTAAATAATAT+AGG - Chr1:12933831-12933850 MsG0180000899.01.T01:CDS 25.0%
!! ACTATTACTGTGATAATGTT+TGG + Chr1:12935424-12935443 None:intergenic 25.0%
!! CTATTACTGTGATAATGTTT+GGG + Chr1:12935423-12935442 None:intergenic 25.0%
!! GCATTACTACAATATGTTTT+GGG - Chr1:12935774-12935793 MsG0180000899.01.T01:intron 25.0%
!! TAGGAATGTTTTCATTTCAA+TGG + Chr1:12932287-12932306 None:intergenic 25.0%
!! TGCATTACTACAATATGTTT+TGG - Chr1:12935773-12935792 MsG0180000899.01.T01:intron 25.0%
!! TTATATTTGTAACTTGCAGT+AGG - Chr1:12934960-12934979 MsG0180000899.01.T01:intron 25.0%
!!! ACTTTAACATTTTTTTGTGC+TGG + Chr1:12932464-12932483 None:intergenic 25.0%
!!! ATTTTAAAATGACATGATGG+AGG - Chr1:12935249-12935268 MsG0180000899.01.T01:intron 25.0%
!!! CTTTAAGTTTGTCTTTTTCT+TGG - Chr1:12933045-12933064 MsG0180000899.01.T01:intron 25.0%
!!! GATATTTGATGATTTTAGGA+TGG - Chr1:12933531-12933550 MsG0180000899.01.T01:intron 25.0%
!!! TTACTTTTCTATTTTGGTAG+GGG - Chr1:12936343-12936362 MsG0180000899.01.T01:intron 25.0%
!!! TTTTAAAATGACATGATGGA+GGG - Chr1:12935250-12935269 MsG0180000899.01.T01:intron 25.0%
AAACATGTTAAGAACTCAGT+TGG - Chr1:12932390-12932409 MsG0180000899.01.T01:intron 30.0%
AAATATGGAAATAGACCAAG+TGG - Chr1:12932724-12932743 MsG0180000899.01.T01:three_prime_UTR 30.0%
AATCTTGTCTTGATTACACT+TGG - Chr1:12933700-12933719 MsG0180000899.01.T01:intron 30.0%
AATTGTCAACAAAGATCTCT+TGG - Chr1:12931981-12932000 MsG0180000899.01.T01:three_prime_UTR 30.0%
ACAATTGTAAGCACATTACA+AGG - Chr1:12934506-12934525 MsG0180000899.01.T01:intron 30.0%
ACTTGGTCTATTTCCATATT+TGG + Chr1:12932725-12932744 None:intergenic 30.0%
AGAGTCTGAAAAAATGACAT+TGG - Chr1:12934115-12934134 MsG0180000899.01.T01:intron 30.0%
ATAATATATGCCAAGACCTT+AGG - Chr1:12932425-12932444 MsG0180000899.01.T01:intron 30.0%
ATTGAACCTTCACACATATA+TGG + Chr1:12934415-12934434 None:intergenic 30.0%
CAATGATGAATAAACAAGGA+AGG + Chr1:12932685-12932704 None:intergenic 30.0%
CAGTTATGTATGAATTCCAA+TGG + Chr1:12934674-12934693 None:intergenic 30.0%
CTTATTCATTGTGACTCATA+CGG - Chr1:12934161-12934180 MsG0180000899.01.T01:intron 30.0%
GACATGATCACATAAGTATT+TGG + Chr1:12935682-12935701 None:intergenic 30.0%
GAGAATTTGTATCTTGAACA+GGG + Chr1:12932513-12932532 None:intergenic 30.0%
GCTCGTAGTAAAAAATCAAT+TGG + Chr1:12932545-12932564 None:intergenic 30.0%
GGTGTTCAACAAAAAATTTG+TGG - Chr1:12933815-12933834 MsG0180000899.01.T01:CDS 30.0%
GTAATGCTTGATAAAATTGG+TGG - Chr1:12932816-12932835 MsG0180000899.01.T01:intron 30.0%
GTATTTATTGAGGGTAAAGT+TGG + Chr1:12932992-12933011 None:intergenic 30.0%
TAATATATGCCAAGACCTTA+GGG - Chr1:12932426-12932445 MsG0180000899.01.T01:intron 30.0%
TATTGTAGTAATGCACATCA+AGG + Chr1:12935768-12935787 None:intergenic 30.0%
TCATAGAGTTCTTAGCAAAA+AGG - Chr1:12935107-12935126 MsG0180000899.01.T01:intron 30.0%
TTATCAGATGAAACTCATGT+GGG + Chr1:12934228-12934247 None:intergenic 30.0%
TTATTCATTGTGACTCATAC+GGG - Chr1:12934162-12934181 MsG0180000899.01.T01:intron 30.0%
TTCTTCTCTCATTTGTCAAA+GGG - Chr1:12935728-12935747 MsG0180000899.01.T01:intron 30.0%
TTTATCAGATGAAACTCATG+TGG + Chr1:12934229-12934248 None:intergenic 30.0%
! AAGAATTGGTCTCTTAAGAT+TGG + Chr1:12934065-12934084 None:intergenic 30.0%
! AGTAATCATGTGCTAGTTTA+AGG + Chr1:12934932-12934951 None:intergenic 30.0%
! ATTCGAATCAAAGCATCAAA+TGG - Chr1:12933942-12933961 MsG0180000899.01.T01:CDS 30.0%
! GTAATCATGTGCTAGTTTAA+GGG + Chr1:12934931-12934950 None:intergenic 30.0%
! TAAAGAAATCAGTTTCGAGT+AGG + Chr1:12935517-12935536 None:intergenic 30.0%
! TGACTCATCCTTCAATTTTA+GGG - Chr1:12933794-12933813 MsG0180000899.01.T01:intron 30.0%
!! AAACGACACTCATTTTGAAA+CGG - Chr1:12933138-12933157 MsG0180000899.01.T01:intron 30.0%
!! ATGCTTTGATTCGAATTTTG+AGG + Chr1:12933938-12933957 None:intergenic 30.0%
!! GGTTTAAATTGATTAGCTTG+AGG + Chr1:12933997-12934016 None:intergenic 30.0%
!! TAAATTGATTAGCTTGAGGA+TGG + Chr1:12933993-12934012 None:intergenic 30.0%
!! TGCTTTGATTCGAATTTTGA+GGG + Chr1:12933937-12933956 None:intergenic 30.0%
!!! AAAATGGTTTTGAGCATAGT+GGG - Chr1:12935029-12935048 MsG0180000899.01.T01:intron 30.0%
!!! ATCAAATGGTCTATTTTTGC+AGG - Chr1:12933956-12933975 MsG0180000899.01.T01:CDS 30.0%
!!! ATGTTTTGGGACAAATTCAA+TGG - Chr1:12935787-12935806 MsG0180000899.01.T01:intron 30.0%
!!! ATTGATTTTTTACTACGAGC+TGG - Chr1:12932545-12932564 MsG0180000899.01.T01:three_prime_UTR 30.0%
!!! TCAAATGCTCTTAGTTTTGT+AGG - Chr1:12935880-12935899 MsG0180000899.01.T01:intron 30.0%
!!! TTGAACTTTGTTTTCTTTGC+AGG - Chr1:12934647-12934666 MsG0180000899.01.T01:intron 30.0%
AAATCCTTGCCAATGTTAGT+TGG - Chr1:12935913-12935932 MsG0180000899.01.T01:intron 35.0%
AAGAGATGGATACACAGAAT+GGG - Chr1:12935054-12935073 MsG0180000899.01.T01:intron 35.0%
AAGCTGTGAATTTAGGAAAC+TGG - Chr1:12931917-12931936 MsG0180000899.01.T01:three_prime_UTR 35.0%
AATACACATCCATTTGTGCT+TGG + Chr1:12936427-12936446 None:intergenic 35.0%
ACATATCTTTGCGCTGAAAA+TGG - Chr1:12933588-12933607 MsG0180000899.01.T01:CDS 35.0%
ACTCAAGGGAAAATGATGAT+AGG - Chr1:12932484-12932503 MsG0180000899.01.T01:three_prime_UTR 35.0%
ACTTGACAATGCTTTCACAT+GGG - Chr1:12934812-12934831 MsG0180000899.01.T01:CDS 35.0%
AGTAAAAAATCAATTGGCCG+TGG + Chr1:12932539-12932558 None:intergenic 35.0%
ATAATCATTGGAGTCTCAAG+TGG - Chr1:12936492-12936511 MsG0180000899.01.T01:CDS 35.0%
ATACACATCCATTTGTGCTT+GGG + Chr1:12936426-12936445 None:intergenic 35.0%
ATAGGGAAACCAAAACTACA+GGG + Chr1:12935366-12935385 None:intergenic 35.0%
ATATCTTTGCGCTGAAAATG+GGG - Chr1:12933590-12933609 MsG0180000899.01.T01:CDS 35.0%
ATGTTAAGAACTCAGTTGGT+AGG - Chr1:12932394-12932413 MsG0180000899.01.T01:intron 35.0%
ATTCTCATGCAACATTTGGA+TGG - Chr1:12936444-12936463 MsG0180000899.01.T01:CDS 35.0%
CATATCTTTGCGCTGAAAAT+GGG - Chr1:12933589-12933608 MsG0180000899.01.T01:CDS 35.0%
CCAAGTGGATGAACTTATAA+CGG - Chr1:12932739-12932758 MsG0180000899.01.T01:three_prime_UTR 35.0%
CTATCTTGATTGACACTTAG+TGG + Chr1:12935610-12935629 None:intergenic 35.0%
CTTCTTCTCTCATTTGTCAA+AGG - Chr1:12935727-12935746 MsG0180000899.01.T01:intron 35.0%
GACACTCATTATGAAACGAA+GGG + Chr1:12932897-12932916 None:intergenic 35.0%
GCTTACAAAGCTGTGAATTT+AGG - Chr1:12931910-12931929 MsG0180000899.01.T01:three_prime_UTR 35.0%
GGAGAATACCAACTTACTAA+CGG - Chr1:12934527-12934546 MsG0180000899.01.T01:intron 35.0%
GGAGAATTTGTATCTTGAAC+AGG + Chr1:12932514-12932533 None:intergenic 35.0%
GGGTTAAATAATATAGGAGG+AGG - Chr1:12933837-12933856 MsG0180000899.01.T01:CDS 35.0%
GTGGGGTTAAATAATATAGG+AGG - Chr1:12933834-12933853 MsG0180000899.01.T01:CDS 35.0%
GTGTATTCTCATGCAACATT+TGG - Chr1:12936440-12936459 MsG0180000899.01.T01:CDS 35.0%
GTTGAACACCCTAAAATTGA+AGG + Chr1:12933805-12933824 None:intergenic 35.0%
TACCAACTTACTAACGGTTA+TGG - Chr1:12934533-12934552 MsG0180000899.01.T01:intron 35.0%
TAGCCTCAAGAACTATTACA+AGG - Chr1:12935627-12935646 MsG0180000899.01.T01:intron 35.0%
TGAAATGTTTCTTGGTTGCT+AGG + Chr1:12933891-12933910 None:intergenic 35.0%
TGACACTCATTATGAAACGA+AGG + Chr1:12932898-12932917 None:intergenic 35.0%
TGTGATCATGTCAAATCCAT+TGG - Chr1:12935690-12935709 MsG0180000899.01.T01:intron 35.0%
TTCTCATGCAACATTTGGAT+GGG - Chr1:12936445-12936464 MsG0180000899.01.T01:CDS 35.0%
! AGATTATGAGGGCACAAATT+GGG - Chr1:12934454-12934473 MsG0180000899.01.T01:intron 35.0%
! GAGAAGAAGTTTAGAATCTG+TGG + Chr1:12935716-12935735 None:intergenic 35.0%
! GTGACTCATCCTTCAATTTT+AGG - Chr1:12933793-12933812 MsG0180000899.01.T01:intron 35.0%
! TGTTAAGAACTCAGTTGGTA+GGG - Chr1:12932395-12932414 MsG0180000899.01.T01:intron 35.0%
! TTTGATTTAAAACCGGCCAT+GGG + Chr1:12933393-12933412 None:intergenic 35.0%
!! ATGTGTTTTGTTGTGTGGAA+TGG + Chr1:12932659-12932678 None:intergenic 35.0%
!! GCTTTGATTCGAATTTTGAG+GGG + Chr1:12933936-12933955 None:intergenic 35.0%
!!! ATATGTTTTGTTTAGCCCCA+TGG - Chr1:12933374-12933393 MsG0180000899.01.T01:intron 35.0%
!!! CAAAATGGTTTTGAGCATAG+TGG - Chr1:12935028-12935047 MsG0180000899.01.T01:intron 35.0%
!!! TTTCTATTTTGGTAGGGGAA+TGG - Chr1:12936348-12936367 MsG0180000899.01.T01:intron 35.0%
AAAAAATCAATTGGCCGTGG+TGG + Chr1:12932536-12932555 None:intergenic 40.0%
AAAAATCAATTGGCCGTGGT+GGG + Chr1:12932535-12932554 None:intergenic 40.0%
AAGATATGCCCAAGCACAAA+TGG - Chr1:12936415-12936434 MsG0180000899.01.T01:CDS 40.0%
ACGTGTTTGTCTATCGTAGT+AGG + Chr1:12932306-12932325 None:intergenic 40.0%
AGAAAAACCACTAAGATCCC+CGG + Chr1:12935857-12935876 None:intergenic 40.0%
AGAGATGGATACACAGAATG+GGG - Chr1:12935055-12935074 MsG0180000899.01.T01:intron 40.0%
AGATACAAATTCTCCCACCA+CGG - Chr1:12932519-12932538 MsG0180000899.01.T01:three_prime_UTR 40.0%
ATCAGTGACTGCAGATTATG+AGG - Chr1:12934442-12934461 MsG0180000899.01.T01:intron 40.0%
CAAGAGATGGATACACAGAA+TGG - Chr1:12935053-12935072 MsG0180000899.01.T01:intron 40.0%
CAAGGGAAAATGATGATAGG+TGG - Chr1:12932487-12932506 MsG0180000899.01.T01:three_prime_UTR 40.0%
CACTTGACAATGCTTTCACA+TGG - Chr1:12934811-12934830 MsG0180000899.01.T01:CDS 40.0%
CCGTTATAAGTTCATCCACT+TGG + Chr1:12932742-12932761 None:intergenic 40.0%
CCTTCACACATATATGGTCA+AGG + Chr1:12934409-12934428 None:intergenic 40.0%
CCTTGACCATATATGTGTGA+AGG - Chr1:12934406-12934425 MsG0180000899.01.T01:intron 40.0%
CTCAAGTGGTTGATCGAAAA+TGG - Chr1:12936506-12936525 MsG0180000899.01.T01:CDS 40.0%
CTGCCTTGTAATAGTTCTTG+AGG + Chr1:12935633-12935652 None:intergenic 40.0%
GATAGGGAAACCAAAACTAC+AGG + Chr1:12935367-12935386 None:intergenic 40.0%
GCAGTTGAAGGTTCTCAAAA+TGG - Chr1:12935013-12935032 MsG0180000899.01.T01:intron 40.0%
GTTGTGTGGAATGGAACATT+GGG + Chr1:12932650-12932669 None:intergenic 40.0%
TAAGCAAACGAAAGAGAGTG+AGG + Chr1:12931895-12931914 None:intergenic 40.0%
TAGACCAACTAACATTGGCA+AGG + Chr1:12935920-12935939 None:intergenic 40.0%
TATCTTTGCGCTGAAAATGG+GGG - Chr1:12933591-12933610 MsG0180000899.01.T01:CDS 40.0%
TCAGTGACTGCAGATTATGA+GGG - Chr1:12934443-12934462 MsG0180000899.01.T01:intron 40.0%
TGGACAAAAAGTTTGCCCAT+GGG - Chr1:12933321-12933340 MsG0180000899.01.T01:intron 40.0%
TGTTGTGTGGAATGGAACAT+TGG + Chr1:12932651-12932670 None:intergenic 40.0%
TTGGACAAAAAGTTTGCCCA+TGG - Chr1:12933320-12933339 MsG0180000899.01.T01:intron 40.0%
TTGTCTCTCACTAAACCCAT+GGG + Chr1:12933339-12933358 None:intergenic 40.0%
TTTCAGCGCAAAGATATGTC+TGG + Chr1:12933587-12933606 None:intergenic 40.0%
TTTCTTACCCTCATGACTTG+AGG + Chr1:12934569-12934588 None:intergenic 40.0%
TTTGTCTCTCACTAAACCCA+TGG + Chr1:12933340-12933359 None:intergenic 40.0%
TTTGTGCTTGGGCATATCTT+TGG + Chr1:12936415-12936434 None:intergenic 40.0%
! AACCCACCTAAACCTTTTGT+TGG - Chr1:12934774-12934793 MsG0180000899.01.T01:CDS 40.0%
! ACAAAAGGTTTAGGTGGGTT+TGG + Chr1:12934774-12934793 None:intergenic 40.0%
! ACCCACCTAAACCTTTTGTT+GGG - Chr1:12934775-12934794 MsG0180000899.01.T01:CDS 40.0%
! AGTTTAGAATCTGTGGCCAA+TGG + Chr1:12935709-12935728 None:intergenic 40.0%
! CAGATTATGAGGGCACAAAT+TGG - Chr1:12934453-12934472 MsG0180000899.01.T01:intron 40.0%
! GTTTGATTTAAAACCGGCCA+TGG + Chr1:12933394-12933413 None:intergenic 40.0%
! TCTCTATCTCCCTGTAGTTT+TGG - Chr1:12935354-12935373 MsG0180000899.01.T01:intron 40.0%
! TGTTTTCTTTGCAGGACCAT+TGG - Chr1:12934655-12934674 MsG0180000899.01.T01:intron 40.0%
! TTCTGGTTGGGAAACATTCA+GGG - Chr1:12933647-12933666 MsG0180000899.01.T01:CDS 40.0%
! TTGATTTAAAACCGGCCATG+GGG + Chr1:12933392-12933411 None:intergenic 40.0%
! TTGTAACTTGCAGTAGGCAT+GGG - Chr1:12934966-12934985 MsG0180000899.01.T01:intron 40.0%
! TTTGTAACTTGCAGTAGGCA+TGG - Chr1:12934965-12934984 MsG0180000899.01.T01:intron 40.0%
!! CAACATTTGGATGGGCTTTT+TGG - Chr1:12936453-12936472 MsG0180000899.01.T01:CDS 40.0%
!! GGTGTATGTGTTTTGTTGTG+TGG + Chr1:12932664-12932683 None:intergenic 40.0%
!! TATTGGCTCATTTGCAGCTT+TGG - Chr1:12933763-12933782 MsG0180000899.01.T01:intron 40.0%
!!! ACATTTTTTTGTGCTGGCAG+AGG + Chr1:12932458-12932477 None:intergenic 40.0%
!!! TATTTTGGTAGGGGAATGGA+CGG - Chr1:12936352-12936371 MsG0180000899.01.T01:intron 40.0%
ACAATTCCGTCAAAGCGAGA+AGG + Chr1:12931967-12931986 None:intergenic 45.0%
ACGGTTGAATGGAGCATACA+AGG - Chr1:12936371-12936390 MsG0180000899.01.T01:intron 45.0%
AGGCAGCTTATGATGTTGTG+AGG - Chr1:12935647-12935666 MsG0180000899.01.T01:intron 45.0%
AGGTTTGAACCCTAAGGTCT+TGG + Chr1:12932438-12932457 None:intergenic 45.0%
ATAGAGCTCCTCAAGTCATG+AGG - Chr1:12934558-12934577 MsG0180000899.01.T01:intron 45.0%
ATGGAACCTTCTCGCTTTGA+CGG - Chr1:12931958-12931977 MsG0180000899.01.T01:three_prime_UTR 45.0%
CAGTGAGAATACCTGTTGGA+TGG - Chr1:12934733-12934752 MsG0180000899.01.T01:CDS 45.0%
CCCCAACAAAAGGTTTAGGT+GGG + Chr1:12934779-12934798 None:intergenic 45.0%
CGCCATAACCGTTAGTAAGT+TGG + Chr1:12934538-12934557 None:intergenic 45.0%
CTGAATGTTTCCCAACCAGA+AGG + Chr1:12933648-12933667 None:intergenic 45.0%
GATGCAGTGAGAATACCTGT+TGG - Chr1:12934729-12934748 MsG0180000899.01.T01:CDS 45.0%
GCATGGGATGAAAGTGATTG+TGG - Chr1:12934982-12935001 MsG0180000899.01.T01:intron 45.0%
GTGAATAAGTGAGCTGTCTG+AGG + Chr1:12932577-12932596 None:intergenic 45.0%
TAGAGCTCCTCAAGTCATGA+GGG - Chr1:12934559-12934578 MsG0180000899.01.T01:intron 45.0%
TCTATGACAGAGACTGTCTG+TGG + Chr1:12935094-12935113 None:intergenic 45.0%
TGAGAATACCTGTTGGATGG+TGG - Chr1:12934736-12934755 MsG0180000899.01.T01:CDS 45.0%
! CCACCTAAACCTTTTGTTGG+GGG - Chr1:12934777-12934796 MsG0180000899.01.T01:CDS 45.0%
! CCCACCTAAACCTTTTGTTG+GGG - Chr1:12934776-12934795 MsG0180000899.01.T01:CDS 45.0%
! CTTCTGGTTGGGAAACATTC+AGG - Chr1:12933646-12933665 MsG0180000899.01.T01:CDS 45.0%
!! AATAAATAGTAAAAAATTAT+TGG - Chr1:12935205-12935224 MsG0180000899.01.T01:intron 5.0%
!! TATATATATATATATATCAA+AGG + Chr1:12935965-12935984 None:intergenic 5.0%
!!! ATAATATAATATAATAGTTT+TGG + Chr1:12932158-12932177 None:intergenic 5.0%
ACCAGAAGGTGAACTACGGT+CGG + Chr1:12933634-12933653 None:intergenic 50.0%
ACCGTAGTTCACCTTCTGGT+TGG - Chr1:12933634-12933653 MsG0180000899.01.T01:CDS 50.0%
CCCAACCAGAAGGTGAACTA+CGG + Chr1:12933638-12933657 None:intergenic 50.0%
CCCCCAACAAAAGGTTTAGG+TGG + Chr1:12934780-12934799 None:intergenic 50.0%
GCTCCCCCAACAAAAGGTTT+AGG + Chr1:12934783-12934802 None:intergenic 50.0%
GTGGACTTGCATGCAGTTGA+AGG - Chr1:12935001-12935020 MsG0180000899.01.T01:intron 50.0%
TAGGGGAATGGACGGTTGAA+TGG - Chr1:12936360-12936379 MsG0180000899.01.T01:intron 50.0%
TGAGCTATCCACCATCCAAC+AGG + Chr1:12934747-12934766 None:intergenic 50.0%
TGGCAGAGGTTTGAACCCTA+AGG + Chr1:12932444-12932463 None:intergenic 50.0%
! ACCTTTTGTTGGGGGAGCTT+TGG - Chr1:12934785-12934804 MsG0180000899.01.T01:CDS 50.0%
! CCGTAGTTCACCTTCTGGTT+GGG - Chr1:12933635-12933654 MsG0180000899.01.T01:CDS 50.0%
! GAGCATAGTGGGACAAGAGA+TGG - Chr1:12935040-12935059 MsG0180000899.01.T01:intron 50.0%
! GCTTGTTGCTGAAGGATGGA+TGG - Chr1:12931939-12931958 MsG0180000899.01.T01:three_prime_UTR 50.0%
! TGGCTCATTTGCAGCTTTGG+AGG - Chr1:12933766-12933785 MsG0180000899.01.T01:intron 50.0%
!! ACTGGCTTGTTGCTGAAGGA+TGG - Chr1:12931935-12931954 MsG0180000899.01.T01:three_prime_UTR 50.0%
!! GGAAACTGGCTTGTTGCTGA+AGG - Chr1:12931931-12931950 MsG0180000899.01.T01:three_prime_UTR 50.0%
!! GGCTCATTTGCAGCTTTGGA+GGG - Chr1:12933767-12933786 MsG0180000899.01.T01:intron 50.0%
!!! GTTTTGTTTAGCCCCATGGC+CGG - Chr1:12933378-12933397 MsG0180000899.01.T01:intron 50.0%
CTTTGCGCTGAAAATGGGGG+AGG - Chr1:12933594-12933613 MsG0180000899.01.T01:CDS 55.0%
GCCAAAGCTCCCCCAACAAA+AGG + Chr1:12934789-12934808 None:intergenic 55.0%
GACAGAGCCGGGGATCTTAG+TGG - Chr1:12935847-12935866 MsG0180000899.01.T01:intron 60.0%
GCCGACCGTAGTTCACCTTC+TGG - Chr1:12933630-12933649 MsG0180000899.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 12931838 12936544 12931838 ID=MsG0180000899.01;Name=MsG0180000899.01
Chr1 mRNA 12931838 12936544 12931838 ID=MsG0180000899.01.T01;Parent=MsG0180000899.01;Name=MsG0180000899.01.T01;_AED=0.48;_eAED=0.72;_QI=0|0|0|1|0.8|0.83|6|522|212
Chr1 exon 12936380 12936544 12936380 ID=MsG0180000899.01.T01:exon:9161;Parent=MsG0180000899.01.T01
Chr1 exon 12934714 12934833 12934714 ID=MsG0180000899.01.T01:exon:9160;Parent=MsG0180000899.01.T01
Chr1 exon 12933802 12933984 12933802 ID=MsG0180000899.01.T01:exon:9159;Parent=MsG0180000899.01.T01
Chr1 exon 12933544 12933713 12933544 ID=MsG0180000899.01.T01:exon:9158;Parent=MsG0180000899.01.T01
Chr1 exon 12932481 12932821 12932481 ID=MsG0180000899.01.T01:exon:9157;Parent=MsG0180000899.01.T01
Chr1 exon 12931838 12932019 12931838 ID=MsG0180000899.01.T01:exon:9156;Parent=MsG0180000899.01.T01
Chr1 CDS 12936380 12936544 12936380 ID=MsG0180000899.01.T01:cds;Parent=MsG0180000899.01.T01
Chr1 CDS 12934714 12934833 12934714 ID=MsG0180000899.01.T01:cds;Parent=MsG0180000899.01.T01
Chr1 CDS 12933802 12933984 12933802 ID=MsG0180000899.01.T01:cds;Parent=MsG0180000899.01.T01
Chr1 CDS 12933544 12933713 12933544 ID=MsG0180000899.01.T01:cds;Parent=MsG0180000899.01.T01
Chr1 CDS 12932821 12932821 12932821 ID=MsG0180000899.01.T01:cds;Parent=MsG0180000899.01.T01
Chr1 three_prime_UTR 12932481 12932820 12932481 ID=MsG0180000899.01.T01:three_prime_utr;Parent=MsG0180000899.01.T01
Chr1 three_prime_UTR 12931838 12932019 12931838 ID=MsG0180000899.01.T01:three_prime_utr;Parent=MsG0180000899.01.T01
Gene Sequence

>MsG0180000899.01.T01

ATGGAATATCGAAACTATTATTTGAGCTTCTTATTGGCGTTATGTCTTTCATGTCCTCACTCTCTTTCGTTTGCTTACAAAGCTGTGAATTTAGGAAACTGGCTTGTTGCTGAAGGATGGATGGAACCTTCTCGCTTTGACGGAATTGTCAACAAAGATCTCTTGGATGGAACTCAAGTACAATTCATGTCAAGAAAGTTCCAGACATATCTTTGCGCTGAAAATGGGGGAGGAACTACTATTGTAGCCGACCGTAGTTCACCTTCTGGTTGGGAAACATTCAGGTTACTAATCCTTGACCATATATGTGTGAAGGTTCAATCGAAGACATCAGTGACTGCAGATTATGAGGGCACAAATTGGGATGACAATGATCCATCAGTTTTTCATATGACAATTGTAAGCACATTACAAGGAGAATACCAACTTACTAACGGTTATGGCGATAGAGCTCCTCAAGTCATGAGGGACCATTGGAATTCATACATAACTGAAGATGATTTCAGATTTATGTCACAAAATGGATTAGATGCAGTGAGAATACCTGTTGGATGGTGGATAGCTCAAGATCCAAACCCACCTAAACCTTTTGTTGGGGGAGCTTTGGCAGCACTTGACAATGCTTTCACATGGGCATAA
Protein sequence

>MsG0180000899.01.T01

MEYRNYYLSFLLALCLSCPHSLSFAYKAVNLGNWLVAEGWMEPSRFDGIVNKDLLDGTQVQFMSRKFQTYLCAENGGGTTIVADRSSPSGWETFRLLILDHICVKVQSKTSVTADYEGTNWDDNDPSVFHMTIVSTLQGEYQLTNGYGDRAPQVMRDHWNSYITEDDFRFMSQNGLDAVRIPVGWWIAQDPNPPKPFVGGALAALDNAFTWA*