AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040805.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040805.01.T01 MTR_7g098750 94.079 152 9 0 1 152 1035 1186 8.17e-97 302
MsG0780040805.01.T01 MTR_7g098750 94.079 152 9 0 1 152 1305 1456 1.48e-96 304
MsG0780040805.01.T01 MTR_7g098370 92.763 152 11 0 1 152 1263 1414 6.65e-96 302
MsG0780040805.01.T01 MTR_7g098740 92.763 152 11 0 1 152 1263 1414 1.44e-95 301
MsG0780040805.01.T01 MTR_7g098760 84.211 152 24 0 1 152 1302 1453 8.65e-87 276
MsG0780040805.01.T01 MTR_7g098320 88.158 152 18 0 1 152 1297 1448 2.65e-84 269
MsG0780040805.01.T01 MTR_7g098300 85.526 152 22 0 1 152 1297 1448 2.55e-80 258
MsG0780040805.01.T01 MTR_4g011640 68.421 152 46 1 1 152 1285 1434 3.53e-72 235
MsG0780040805.01.T01 MTR_4g011630 64.474 152 52 1 1 152 1288 1437 2.48e-69 227
MsG0780040805.01.T01 MTR_2g102640 64.474 152 53 1 1 152 1280 1430 5.85e-67 220
MsG0780040805.01.T01 MTR_2g102660 63.816 152 54 1 1 152 1277 1427 4.10e-66 218
MsG0780040805.01.T01 MTR_2g102670 63.816 152 54 1 1 152 1260 1410 5.06e-66 217
MsG0780040805.01.T01 MTR_1g011650 61.842 152 55 1 1 152 1299 1447 1.80e-63 210
MsG0780040805.01.T01 MTR_1g011640 61.842 152 55 1 1 152 1276 1424 2.10e-63 210
MsG0780040805.01.T01 MTR_4g011620 65.132 152 51 1 1 152 1278 1427 2.50e-59 198
MsG0780040805.01.T01 MTR_7g098780 56.579 152 66 0 1 152 1267 1418 6.71e-59 197
MsG0780040805.01.T01 MTR_4g113070 56.863 153 65 1 1 152 1266 1418 2.77e-56 189
MsG0780040805.01.T01 MTR_7g098800 62.500 152 57 0 1 152 1225 1376 1.57e-55 187
MsG0780040805.01.T01 MTR_1g050525 53.247 154 69 2 1 152 1316 1468 1.09e-49 171
MsG0780040805.01.T01 MTR_7g104130 51.613 155 70 2 1 152 1305 1457 4.16e-49 169
MsG0780040805.01.T01 MTR_7g098890 60.526 152 60 0 1 152 1205 1356 5.48e-49 168
MsG0780040805.01.T01 MTR_4g123850 46.053 152 82 0 1 152 1280 1431 7.45e-48 165
MsG0780040805.01.T01 MTR_7g104110 50.327 153 75 1 1 152 1297 1449 1.94e-47 164
MsG0780040805.01.T01 MTR_7g407080 92.500 80 6 0 1 80 501 580 2.22e-46 159
MsG0780040805.01.T01 MTR_7g104150 45.098 153 83 1 1 152 1305 1457 3.33e-45 157
MsG0780040805.01.T01 MTR_1g492950 50.980 153 74 1 1 152 1316 1468 9.21e-45 156
MsG0780040805.01.T01 MTR_3g107870 55.921 152 64 1 1 152 1312 1460 1.78e-44 155
MsG0780040805.01.T01 MTR_7g104100 45.098 153 83 1 1 152 1303 1455 3.23e-42 149
MsG0780040805.01.T01 MTR_2g101090 51.299 154 73 2 1 152 1293 1446 4.49e-41 146
MsG0780040805.01.T01 MTR_3g463680 45.223 157 81 1 1 152 1265 1421 6.87e-41 145
MsG0780040805.01.T01 MTR_8g014360 50.000 154 75 1 1 152 1327 1480 9.32e-40 142
MsG0780040805.01.T01 MTR_7g098750 96.875 64 2 0 1 64 1305 1368 1.31e-36 133
MsG0780040805.01.T01 MTR_5g070320 45.223 157 81 1 1 152 993 1149 1.07e-34 127
MsG0780040805.01.T01 MTR_5g070320 45.223 157 81 1 1 152 1089 1245 1.26e-34 127
MsG0780040805.01.T01 MTR_5g070320 45.223 157 81 1 1 152 1280 1436 1.50e-34 127
MsG0780040805.01.T01 MTR_7g098790 43.902 123 65 2 34 152 194 316 1.12e-29 110
MsG0780040805.01.T01 MTR_7g098310 80.000 55 11 0 98 152 16 70 2.84e-24 89.7
MsG0780040805.01.T01 MTR_7g104130 50.794 63 31 0 1 63 1305 1367 1.38e-16 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040805.01.T01 AT1G15520 63.816 152 53 1 1 152 1274 1423 1.14e-66 219
MsG0780040805.01.T01 AT2G26910 60.390 154 59 1 1 152 1267 1420 3.28e-62 206
MsG0780040805.01.T01 AT1G66950 54.248 153 69 1 1 152 1302 1454 2.99e-52 178
MsG0780040805.01.T01 AT2G36380 53.595 153 70 1 1 152 1301 1453 2.89e-51 175
MsG0780040805.01.T01 AT2G29940 53.642 151 70 0 2 152 1276 1426 1.74e-48 167
MsG0780040805.01.T01 AT3G16340 48.701 154 77 1 1 152 1258 1411 3.77e-48 166
MsG0780040805.01.T01 AT3G16340 48.701 154 77 1 1 152 1263 1416 4.11e-48 166
MsG0780040805.01.T01 AT1G15210 51.948 154 72 1 1 152 1289 1442 1.06e-47 165
MsG0780040805.01.T01 AT1G59870 51.299 154 73 1 1 152 1316 1469 2.36e-47 164
MsG0780040805.01.T01 AT4G15215 44.079 152 85 0 1 152 1246 1397 2.41e-44 155
MsG0780040805.01.T01 AT4G15215 44.079 152 85 0 1 152 1239 1390 2.68e-44 155
MsG0780040805.01.T01 AT4G15215 44.079 152 85 0 1 152 1243 1394 2.68e-44 155
MsG0780040805.01.T01 AT4G15215 44.079 152 85 0 1 152 1243 1394 2.68e-44 155
MsG0780040805.01.T01 AT4G15215 44.079 152 85 0 1 152 1243 1394 2.68e-44 155
MsG0780040805.01.T01 AT4G15215 44.079 152 85 0 1 152 1243 1394 2.68e-44 155
MsG0780040805.01.T01 AT4G15215 44.079 152 85 0 1 152 1243 1394 2.68e-44 155
MsG0780040805.01.T01 AT4G15215 44.079 152 85 0 1 152 1236 1387 3.13e-44 155
MsG0780040805.01.T01 AT4G15215 44.079 152 85 0 1 152 1236 1387 3.13e-44 155
MsG0780040805.01.T01 AT4G15215 44.079 152 85 0 1 152 1236 1387 3.13e-44 155
MsG0780040805.01.T01 AT4G15215 44.079 152 85 0 1 152 1236 1387 3.13e-44 155
MsG0780040805.01.T01 AT4G15230 41.722 151 88 0 2 152 1250 1400 1.10e-41 147
MsG0780040805.01.T01 AT4G15230 41.722 151 88 0 2 152 1250 1400 1.10e-41 147
MsG0780040805.01.T01 AT4G15233 42.763 152 87 0 1 152 1098 1249 1.18e-41 147
MsG0780040805.01.T01 AT4G15233 42.763 152 87 0 1 152 1098 1249 1.18e-41 147
MsG0780040805.01.T01 AT4G15233 42.763 152 87 0 1 152 1179 1330 1.33e-41 147
MsG0780040805.01.T01 AT4G15233 42.763 152 87 0 1 152 1179 1330 1.33e-41 147
MsG0780040805.01.T01 AT4G15233 42.763 152 87 0 1 152 1223 1374 1.55e-41 147
MsG0780040805.01.T01 AT4G15233 42.763 152 87 0 1 152 1232 1383 1.57e-41 147
MsG0780040805.01.T01 AT4G15233 42.763 152 87 0 1 152 1241 1392 1.68e-41 147
MsG0780040805.01.T01 AT3G30842 42.105 152 85 1 1 152 1270 1418 8.08e-41 145
MsG0780040805.01.T01 AT4G15236 42.105 152 88 0 1 152 1239 1390 1.57e-40 144
MsG0780040805.01.T01 AT3G53480 44.737 152 84 0 1 152 1299 1450 2.49e-40 144
MsG0780040805.01.T01 AT4G15230 41.722 151 88 0 2 152 1171 1321 1.57e-39 141
MsG0780040805.01.T01 AT2G37280 40.789 152 90 0 1 152 1104 1255 1.51e-38 139
MsG0780040805.01.T01 AT2G37280 40.789 152 90 0 1 152 1291 1442 1.71e-38 139
MsG0780040805.01.T01 AT2G37280 40.789 152 90 0 1 152 1262 1413 2.18e-38 138
MsG0780040805.01.T01 AT4G15233 34.426 122 56 1 1 98 1241 1362 6.41e-22 91.3
MsG0780040805.01.T01 AT4G15230 38.235 68 41 1 2 68 1250 1317 4.41e-13 66.2

Find 37 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTGCATTCTATTCAGTA+TGG 0.330343 7:-82727855 MsG0780040805.01.T01:CDS
GGTTTGGTGGCTTCACAATA+TGG 0.333013 7:-82727540 MsG0780040805.01.T01:CDS
TAGTGGAAATGTGGTTGTTT+GGG 0.344847 7:+82727884 None:intergenic
TTTATAGTTTATGCTATGAT+TGG 0.358461 7:-82728006 None:intergenic
AATGTGGTTGTTTGGGGTCA+TGG 0.367073 7:+82727891 None:intergenic
CCAGTTGCATGGAGTTTGTA+TGG 0.367386 7:-82727561 MsG0780040805.01.T01:CDS
CCATACAAACTCCATGCAAC+TGG 0.395944 7:+82727561 None:intergenic
TGCATGGAGTTTGTATGGTT+TGG 0.398197 7:-82727556 MsG0780040805.01.T01:CDS
CACTGCCATCATACCATAGA+AGG 0.398537 7:+82727915 None:intergenic
ATAGTGGAAATGTGGTTGTT+TGG 0.422047 7:+82727883 None:intergenic
TGTGGTGGAGGTGGTACAGT+TGG 0.422501 7:-82727590 MsG0780040805.01.T01:CDS
GAAATTTAGTTAGATATGCT+TGG 0.433819 7:-82727239 MsG0780040805.01.T01:three_prime_UTR
AGGTGAAATACATGAAGAAC+AGG 0.463576 7:+82727950 None:intergenic
ATGGAGTTTGTATGGTTTGG+TGG 0.464180 7:-82727553 MsG0780040805.01.T01:CDS
ATTGAACATCTTGATTGCTA+TGG 0.469313 7:+82727371 None:intergenic
AAGTTCAAACTTACAGGTCT+TGG 0.469498 7:+82727808 None:intergenic
TCAGTATGGAATCTCTTCTC+AGG 0.470732 7:-82727841 MsG0780040805.01.T01:CDS
TACCATAGAAGGTAAAATAG+AGG 0.495626 7:+82727926 None:intergenic
GTGGTGGAGGTGGTACAGTT+GGG 0.502586 7:-82727589 MsG0780040805.01.T01:CDS
GAATGCAGAGGAAACTATAG+TGG 0.503627 7:+82727867 None:intergenic
CAAACTCCATGCAACTGGAT+TGG 0.519581 7:+82727566 None:intergenic
ACACACAAAATAAATGATGT+GGG 0.524912 7:+82727281 None:intergenic
GAATTCCAGTGTGGTGGAGG+TGG 0.528963 7:-82727599 MsG0780040805.01.T01:CDS
AGAGAATTCCAGTGTGGTGG+AGG 0.533505 7:-82727602 MsG0780040805.01.T01:CDS
CACACACAAAATAAATGATG+TGG 0.536542 7:+82727280 None:intergenic
CTGTACCACCTCCACCACAC+TGG 0.576057 7:+82727594 None:intergenic
AACAGAGAATTCCAGTGTGG+TGG 0.583120 7:-82727605 MsG0780040805.01.T01:intron
CTTCTATGGTATGATGGCAG+TGG 0.587926 7:-82727914 MsG0780040805.01.T01:CDS
GTTGGGCCAATCCAGTTGCA+TGG 0.592105 7:-82727572 MsG0780040805.01.T01:CDS
ATAGAAGGTAAAATAGAGGA+AGG 0.602640 7:+82727930 None:intergenic
AGGAAACTATAGTGGAAATG+TGG 0.617626 7:+82727875 None:intergenic
CAAAACATTGAAACCAGTGA+TGG 0.617948 7:-82727507 MsG0780040805.01.T01:CDS
AGTGGAAATGTGGTTGTTTG+GGG 0.640507 7:+82727885 None:intergenic
CACACAAAATAAATGATGTG+GGG 0.656451 7:+82727282 None:intergenic
CCATACTGAATAGAATGCAG+AGG 0.663432 7:+82727855 None:intergenic
CAGTGATGGTAGCAAAACAG+TGG 0.667434 7:-82727493 MsG0780040805.01.T01:CDS
GCTAACAGAGAATTCCAGTG+TGG 0.745630 7:-82727608 MsG0780040805.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAATTTAAAATAAAACAAT+GGG + Chr7:82727514-82727533 None:intergenic 10.0%
!!! TGAAATTTAAAATAAAACAA+TGG + Chr7:82727515-82727534 None:intergenic 10.0%
!!! TTCAATTTTACTTTTTGTAT+AGG - Chr7:82727885-82727904 MsG0780040805.01.T01:CDS 15.0%
!!! GAATTTTTGAGAAATTACTT+TGG - Chr7:82727745-82727764 MsG0780040805.01.T01:intron 20.0%
!!! TTATAGTACATTGTTTTTCT+TGG - Chr7:82727574-82727593 MsG0780040805.01.T01:CDS 20.0%
! ACACACAAAATAAATGATGT+GGG + Chr7:82727935-82727954 None:intergenic 25.0%
! GAAATTTAGTTAGATATGCT+TGG - Chr7:82727974-82727993 MsG0780040805.01.T01:CDS 25.0%
! TAAAATGCCCTAAGAATTTA+AGG + Chr7:82727431-82727450 None:intergenic 25.0%
! TGAACTTCCTTAAATTCTTA+GGG - Chr7:82727421-82727440 MsG0780040805.01.T01:CDS 25.0%
! TTGAACTTCCTTAAATTCTT+AGG - Chr7:82727420-82727439 MsG0780040805.01.T01:CDS 25.0%
!! ATTTCCAATAGTTTTTGCTT+TGG - Chr7:82727813-82727832 MsG0780040805.01.T01:intron 25.0%
!! TCAAGATGTTCAATTTTCAA+AGG - Chr7:82727851-82727870 MsG0780040805.01.T01:CDS 25.0%
AAGACCAAAGCAAAAACTAT+TGG + Chr7:82727820-82727839 None:intergenic 30.0%
ATAGAAGGTAAAATAGAGGA+AGG + Chr7:82727286-82727305 None:intergenic 30.0%
ATTGAACATCTTGATTGCTA+TGG + Chr7:82727845-82727864 None:intergenic 30.0%
CACACAAAATAAATGATGTG+GGG + Chr7:82727934-82727953 None:intergenic 30.0%
CACACACAAAATAAATGATG+TGG + Chr7:82727936-82727955 None:intergenic 30.0%
TACCATAGAAGGTAAAATAG+AGG + Chr7:82727290-82727309 None:intergenic 30.0%
TTAAGGAAGTTCAAACTTAC+AGG + Chr7:82727414-82727433 None:intergenic 30.0%
! TTCCTCTATTTTACCTTCTA+TGG - Chr7:82727285-82727304 MsG0780040805.01.T01:three_prime_UTR 30.0%
! TTTTACCTTCTATGGTATGA+TGG - Chr7:82727293-82727312 MsG0780040805.01.T01:three_prime_UTR 30.0%
!! TAGTACATTGTTTTTCTTGG+TGG - Chr7:82727577-82727596 MsG0780040805.01.T01:CDS 30.0%
AAGTTCAAACTTACAGGTCT+TGG + Chr7:82727408-82727427 None:intergenic 35.0%
AGGTGAAATACATGAAGAAC+AGG + Chr7:82727266-82727285 None:intergenic 35.0%
CAAAACATTGAAACCAGTGA+TGG - Chr7:82727706-82727725 MsG0780040805.01.T01:intron 35.0%
TAGTGGAAATGTGGTTGTTT+GGG + Chr7:82727332-82727351 None:intergenic 35.0%
! AGGAAACTATAGTGGAAATG+TGG + Chr7:82727341-82727360 None:intergenic 35.0%
! ATAGTGGAAATGTGGTTGTT+TGG + Chr7:82727333-82727352 None:intergenic 35.0%
! GGTTTCAAGCATGATTTTCT+AGG - Chr7:82727766-82727785 MsG0780040805.01.T01:intron 35.0%
!! ATTGTTTTTCTTGGTGGCTT+TGG - Chr7:82727583-82727602 MsG0780040805.01.T01:CDS 35.0%
!! GGGGTATTTTCTTCTTTAGA+TGG + Chr7:82727915-82727934 None:intergenic 35.0%
AGTGGAAATGTGGTTGTTTG+GGG + Chr7:82727331-82727350 None:intergenic 40.0%
CCATACTGAATAGAATGCAG+AGG + Chr7:82727361-82727380 None:intergenic 40.0%
CCTCTGCATTCTATTCAGTA+TGG - Chr7:82727358-82727377 MsG0780040805.01.T01:CDS 40.0%
GAATGCAGAGGAAACTATAG+TGG + Chr7:82727349-82727368 None:intergenic 40.0%
TCAGTATGGAATCTCTTCTC+AGG - Chr7:82727372-82727391 MsG0780040805.01.T01:CDS 40.0%
TGCATGGAGTTTGTATGGTT+TGG - Chr7:82727657-82727676 MsG0780040805.01.T01:intron 40.0%
! ATGGAGTTTGTATGGTTTGG+TGG - Chr7:82727660-82727679 MsG0780040805.01.T01:intron 40.0%
! GGACTTTGGTCAAAGTTTTG+TGG - Chr7:82727238-82727257 MsG0780040805.01.T01:three_prime_UTR 40.0%
!! CAAGCATGATTTTCTAGGAG+TGG - Chr7:82727771-82727790 MsG0780040805.01.T01:intron 40.0%
!!! GATTGGTTTTGAGTGGACTT+TGG - Chr7:82727224-82727243 MsG0780040805.01.T01:three_prime_UTR 40.0%
AACAGAGAATTCCAGTGTGG+TGG - Chr7:82727608-82727627 MsG0780040805.01.T01:intron 45.0%
CAAACTCCATGCAACTGGAT+TGG + Chr7:82727650-82727669 None:intergenic 45.0%
CACTGCCATCATACCATAGA+AGG + Chr7:82727301-82727320 None:intergenic 45.0%
CAGTGATGGTAGCAAAACAG+TGG - Chr7:82727720-82727739 MsG0780040805.01.T01:intron 45.0%
CCAGTTGCATGGAGTTTGTA+TGG - Chr7:82727652-82727671 MsG0780040805.01.T01:intron 45.0%
CCATACAAACTCCATGCAAC+TGG + Chr7:82727655-82727674 None:intergenic 45.0%
CTTCTATGGTATGATGGCAG+TGG - Chr7:82727299-82727318 MsG0780040805.01.T01:three_prime_UTR 45.0%
GCTAACAGAGAATTCCAGTG+TGG - Chr7:82727605-82727624 MsG0780040805.01.T01:intron 45.0%
! AATGTGGTTGTTTGGGGTCA+TGG + Chr7:82727325-82727344 None:intergenic 45.0%
! CACTGTTTTGCTACCATCAC+TGG + Chr7:82727722-82727741 None:intergenic 45.0%
!! GGTTTGGTGGCTTCACAATA+TGG - Chr7:82727673-82727692 MsG0780040805.01.T01:intron 45.0%
AGAGAATTCCAGTGTGGTGG+AGG - Chr7:82727611-82727630 MsG0780040805.01.T01:intron 50.0%
GTTGGGCCAATCCAGTTGCA+TGG - Chr7:82727641-82727660 MsG0780040805.01.T01:intron 55.0%
! GAATTCCAGTGTGGTGGAGG+TGG - Chr7:82727614-82727633 MsG0780040805.01.T01:intron 55.0%
! GTGGTGGAGGTGGTACAGTT+GGG - Chr7:82727624-82727643 MsG0780040805.01.T01:intron 55.0%
! TGTGGTGGAGGTGGTACAGT+TGG - Chr7:82727623-82727642 MsG0780040805.01.T01:intron 55.0%
CTGTACCACCTCCACCACAC+TGG + Chr7:82727622-82727641 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 82727222 82728013 82727222 ID=MsG0780040805.01;Name=MsG0780040805.01
Chr7 mRNA 82727222 82728013 82727222 ID=MsG0780040805.01.T01;Parent=MsG0780040805.01;Name=MsG0780040805.01.T01;_AED=0.49;_eAED=0.49;_QI=0|1|0.5|1|1|0.5|2|134|152
Chr7 exon 82727222 82727622 82727222 ID=MsG0780040805.01.T01:exon:19447;Parent=MsG0780040805.01.T01
Chr7 exon 82727822 82728013 82727822 ID=MsG0780040805.01.T01:exon:19446;Parent=MsG0780040805.01.T01
Chr7 CDS 82727822 82728013 82727822 ID=MsG0780040805.01.T01:cds;Parent=MsG0780040805.01.T01
Chr7 CDS 82727356 82727622 82727356 ID=MsG0780040805.01.T01:cds;Parent=MsG0780040805.01.T01
Chr7 three_prime_UTR 82727222 82727355 82727222 ID=MsG0780040805.01.T01:three_prime_utr;Parent=MsG0780040805.01.T01
Gene Sequence

>MsG0780040805.01.T01

ATGATTGGTTTTGAGTGGACTTTGGTCAAAGTTTTGTGGTACCTGTTCTTCATGTATTTCACCTTCCTCTATTTTACCTTCTATGGTATGATGGCAGTGGCCATGACCCCAAACAACCACATTTCCACTATAGTTTCCTCTGCATTCTATTCAGTATGGAATCTCTTCTCAGGATTCATAGTTCCAAGACCTAGAATTCCAGTGTGGTGGAGGTGGTACAGTTGGGCCAATCCAGTTGCATGGAGTTTGTATGGTTTGGTGGCTTCACAATATGGAGATGTAAAACAAAACATTGAAACCAGTGATGGTAGCAAAACAGTGGAAGAATTTTTGAGAAATTACTTTGGTTTCAAGCATGATTTTCTAGGAGTGGTTGCACTTGTCAATGTTGCATTTCCAATAGTTTTTGCTTTGGTCTTTGCCATAGCAATCAAGATGTTCAATTTTCAAAGGCGTTAA

Protein sequence

>MsG0780040805.01.T01

MIGFEWTLVKVLWYLFFMYFTFLYFTFYGMMAVAMTPNNHISTIVSSAFYSVWNLFSGFIVPRPRIPVWWRWYSWANPVAWSLYGLVASQYGDVKQNIETSDGSKTVEEFLRNYFGFKHDFLGVVALVNVAFPIVFALVFAIAIKMFNFQRR*