Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001272.01.T01 | PNX84970.1 | 56.081 | 148 | 26 | 5 | 1 | 109 | 1 | 148 | 8.23E-36 | 129 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001272.01.T01 | Q8GZP5 | 42.177 | 147 | 46 | 5 | 1 | 108 | 345 | 491 | 1.19E-24 | 99.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001272.01.T01 | A0A2K3MFZ0 | 56.081 | 148 | 26 | 5 | 1 | 109 | 1 | 148 | 3.93e-36 | 129 |
| MsG0180001272.01.T01 | Q7X9B2 | 60.811 | 148 | 19 | 5 | 1 | 109 | 338 | 485 | 1.59e-35 | 136 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048842.01 | MsG0180001272.01 | -0.822213 | 2.671741e-53 | 2.221875e-50 |
| MsG0180000076.01 | MsG0180001272.01 | 0.801330 | 9.475124e-49 | 4.512681e-46 |
| MsG0180000373.01 | MsG0180001272.01 | 0.800481 | 1.412424e-48 | 6.583676e-46 |
| MsG0180001272.01 | MsG0180004027.01 | 0.801758 | 7.739958e-49 | 3.726223e-46 |
| MsG0180001272.01 | MsG0180004126.01 | 0.805634 | 1.214593e-49 | 6.458312e-47 |
| MsG0180001272.01 | MsG0180004510.01 | 0.828311 | 9.645370e-55 | 9.551565e-52 |
| MsG0180001272.01 | MsG0180006026.01 | 0.819926 | 8.984803e-53 | 7.009157e-50 |
| MsG0180001272.01 | MsG0280006310.01 | 0.810826 | 9.503982e-51 | 5.788616e-48 |
| MsG0180001272.01 | MsG0280006884.01 | 0.802323 | 5.923659e-49 | 2.893417e-46 |
| MsG0180001272.01 | MsG0280007983.01 | 0.808811 | 2.578447e-50 | 1.488682e-47 |
| MsG0180001272.01 | MsG0280010868.01 | 0.812801 | 3.531644e-51 | 2.266850e-48 |
| MsG0180001272.01 | MsG0380013041.01 | 0.802867 | 4.575053e-49 | 2.265769e-46 |
| MsG0180001272.01 | MsG0380014754.01 | 0.839373 | 1.647011e-57 | 2.270058e-54 |
| MsG0180001272.01 | MsG0380015548.01 | 0.804191 | 2.431609e-49 | 1.245780e-46 |
| MsG0180001272.01 | MsG0380017024.01 | 0.824493 | 7.834204e-54 | 6.950069e-51 |
| MsG0180001272.01 | MsG0380018007.01 | 0.806949 | 6.414854e-50 | 3.527867e-47 |
| MsG0180001272.01 | MsG0480019588.01 | 0.806833 | 6.789432e-50 | 3.722668e-47 |
| MsG0180001272.01 | MsG0480019635.01 | 0.805486 | 1.304205e-49 | 6.908540e-47 |
| MsG0180001272.01 | MsG0480020263.01 | 0.839086 | 1.955685e-57 | 2.671683e-54 |
| MsG0180001272.01 | MsG0480022167.01 | 0.801315 | 9.540794e-49 | 4.542274e-46 |
| MsG0180001272.01 | MsG0480022407.01 | 0.813801 | 2.129777e-51 | 1.404599e-48 |
| MsG0180001272.01 | MsG0480023324.01 | 0.804891 | 1.737354e-49 | 9.064307e-47 |
| MsG0180001272.01 | MsG0480023450.01 | 0.810959 | 8.894310e-51 | 5.436980e-48 |
| MsG0180001272.01 | MsG0580024478.01 | 0.842067 | 3.242003e-58 | 4.858431e-55 |
| MsG0180001272.01 | MsG0580025385.01 | 0.809073 | 2.265861e-50 | 1.317461e-47 |
| MsG0180001272.01 | MsG0580025822.01 | 0.815326 | 9.791946e-52 | 6.730227e-49 |
| MsG0180001272.01 | MsG0680031616.01 | 0.804362 | 2.240754e-49 | 1.153087e-46 |
| MsG0180001272.01 | MsG0780040543.01 | 0.806206 | 9.206952e-50 | 4.968044e-47 |
| MsG0180001272.01 | MsG0780041363.01 | 0.821375 | 4.176008e-53 | 3.391344e-50 |
| MsG0180001272.01 | MsG0880042927.01 | 0.816238 | 6.131362e-52 | 4.320877e-49 |
| MsG0180001272.01 | MsG0880046165.01 | 0.806347 | 8.595729e-50 | 4.654693e-47 |
| MsG0180001272.01 | MsG0880047466.01 | 0.800687 | 1.282156e-48 | 6.007783e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001272.01.T01 | MTR_1g034320 | 60.811 | 148 | 19 | 5 | 1 | 109 | 338 | 485 | 4.03e-39 | 136 |
| MsG0180001272.01.T01 | MTR_1g034330 | 51.351 | 148 | 33 | 5 | 1 | 109 | 342 | 489 | 6.48e-31 | 114 |
| MsG0180001272.01.T01 | MTR_4g068550 | 47.297 | 148 | 39 | 3 | 1 | 109 | 334 | 481 | 3.68e-29 | 109 |
| MsG0180001272.01.T01 | MTR_3g111530 | 41.406 | 128 | 36 | 3 | 1 | 89 | 412 | 539 | 7.56e-24 | 94.7 |
| MsG0180001272.01.T01 | MTR_1g021652 | 38.095 | 147 | 52 | 4 | 1 | 108 | 377 | 523 | 1.75e-23 | 93.6 |
| MsG0180001272.01.T01 | MTR_2g087890 | 45.161 | 62 | 33 | 1 | 3 | 63 | 344 | 405 | 6.80e-13 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001272.01.T01 | AT5G42650 | 37.415 | 147 | 52 | 5 | 1 | 107 | 371 | 517 | 1.48e-20 | 85.5 |
| MsG0180001272.01.T01 | AT4G15440 | 54.688 | 64 | 28 | 1 | 1 | 63 | 246 | 309 | 2.65e-18 | 79.0 |
Find 14 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTCGCGACAAAAGATTCT+AGG | 0.247314 | 1:+18615874 | MsG0180001272.01.T01:CDS |
| TTTGTTGTGGCGAAGGGTTG+TGG | 0.357102 | 1:+18615916 | MsG0180001272.01.T01:CDS |
| TTGTTGTGGCGAAGGGTTGT+GGG | 0.408783 | 1:+18615917 | MsG0180001272.01.T01:CDS |
| GTGGCGAAGGGTTGTGGGGA+AGG | 0.441117 | 1:+18615922 | MsG0180001272.01.T01:CDS |
| TGCATATTGATAGGCACAGC+TGG | 0.481410 | 1:-18615771 | None:intergenic |
| TTTGTGTAGATTGTTATTGG+TGG | 0.498822 | 1:+18616043 | MsG0180001272.01.T01:intron |
| CATTCAAACACTAGAAGCCT+TGG | 0.530423 | 1:-18616144 | None:intergenic |
| TTTCGCGACAAAAGATTCTA+GGG | 0.530911 | 1:+18615875 | MsG0180001272.01.T01:CDS |
| TAGACAACTGATTTAACTAA+CGG | 0.540239 | 1:-18615729 | None:intergenic |
| TGTTGTGGCGAAGGGTTGTG+GGG | 0.603915 | 1:+18615918 | MsG0180001272.01.T01:CDS |
| AAGCCTTGGTGAGTGAGTAA+TGG | 0.608695 | 1:-18616130 | None:intergenic |
| CCTTCGCCACAACAAAACTC+AGG | 0.634017 | 1:-18615909 | None:intergenic |
| TTACCATTACTCACTCACCA+AGG | 0.724466 | 1:+18616127 | MsG0180001272.01.T01:CDS |
| AATCGTGAAGAGTCACGACG+CGG | 0.770505 | 1:+18615809 | MsG0180001272.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAAAGGGTGAAATGATATT+TGG | + | Chr1:18615843-18615862 | MsG0180001272.01.T01:CDS | 25.0% |
| !! | TAGACAACTGATTTAACTAA+CGG | - | Chr1:18615732-18615751 | None:intergenic | 25.0% |
| TTGAGACTAAAAATGTTGCT+TGG | + | Chr1:18616096-18616115 | MsG0180001272.01.T01:CDS | 30.0% | |
| ! | AAGTATGTTTTGTGGTCTAA+TGG | + | Chr1:18615957-18615976 | MsG0180001272.01.T01:intron | 30.0% |
| ! | AGTATGTTTTGTGGTCTAAT+GGG | + | Chr1:18615958-18615977 | MsG0180001272.01.T01:intron | 30.0% |
| ! | GCTTTTGTGTAGATTGTTAT+TGG | + | Chr1:18616040-18616059 | MsG0180001272.01.T01:intron | 30.0% |
| ! | TTTGTGTAGATTGTTATTGG+TGG | + | Chr1:18616043-18616062 | MsG0180001272.01.T01:intron | 30.0% |
| !!! | AAGTGTTGAAGTATGTTTTG+TGG | + | Chr1:18615949-18615968 | MsG0180001272.01.T01:intron | 30.0% |
| !!! | TTCACCCTTTTTTATCTCAA+AGG | - | Chr1:18615835-18615854 | None:intergenic | 30.0% |
| TTTCGCGACAAAAGATTCTA+GGG | + | Chr1:18615875-18615894 | MsG0180001272.01.T01:CDS | 35.0% | |
| !! | TCTCGCTTTTGCATATTGAT+AGG | - | Chr1:18615783-18615802 | None:intergenic | 35.0% |
| CTTTCGCGACAAAAGATTCT+AGG | + | Chr1:18615874-18615893 | MsG0180001272.01.T01:CDS | 40.0% | |
| TTACCATTACTCACTCACCA+AGG | + | Chr1:18616127-18616146 | MsG0180001272.01.T01:CDS | 40.0% | |
| ! | AATCTTTTGTCGCGAAAGGT+TGG | - | Chr1:18615872-18615891 | None:intergenic | 40.0% |
| ! | CTAGAATCTTTTGTCGCGAA+AGG | - | Chr1:18615876-18615895 | None:intergenic | 40.0% |
| !! | GCGGCCTTTGAGATAAAAAA+GGG | + | Chr1:18615828-18615847 | MsG0180001272.01.T01:CDS | 40.0% |
| AAGCCTTGGTGAGTGAGTAA+TGG | - | Chr1:18616133-18616152 | None:intergenic | 45.0% | |
| AGAAACAGAAGAGCCTTCAC+CGG | + | Chr1:18615983-18616002 | MsG0180001272.01.T01:intron | 45.0% | |
| AGCACAACAAGATTCTTCCC+AGG | - | Chr1:18616023-18616042 | None:intergenic | 45.0% | |
| CATTGCTTATTCTCCGGTGA+AGG | - | Chr1:18615999-18616018 | None:intergenic | 45.0% | |
| CCAGGACATTGCTTATTCTC+CGG | - | Chr1:18616005-18616024 | None:intergenic | 45.0% | |
| CGGAGAATAAGCAATGTCCT+GGG | + | Chr1:18616003-18616022 | MsG0180001272.01.T01:intron | 45.0% | |
| TGCATATTGATAGGCACAGC+TGG | - | Chr1:18615774-18615793 | None:intergenic | 45.0% | |
| !! | CGCGGCCTTTGAGATAAAAA+AGG | + | Chr1:18615827-18615846 | MsG0180001272.01.T01:CDS | 45.0% |
| !!! | CTGAGTTTTGTTGTGGCGAA+GGG | + | Chr1:18615910-18615929 | MsG0180001272.01.T01:CDS | 45.0% |
| !!! | GACGATCCTGAGTTTTGTTG+TGG | + | Chr1:18615903-18615922 | MsG0180001272.01.T01:CDS | 45.0% |
| AATCGTGAAGAGTCACGACG+CGG | + | Chr1:18615809-18615828 | MsG0180001272.01.T01:CDS | 50.0% | |
| CCGGAGAATAAGCAATGTCC+TGG | + | Chr1:18616002-18616021 | MsG0180001272.01.T01:intron | 50.0% | |
| CCTTCGCCACAACAAAACTC+AGG | - | Chr1:18615912-18615931 | None:intergenic | 50.0% | |
| TTGTTGTGGCGAAGGGTTGT+GGG | + | Chr1:18615917-18615936 | MsG0180001272.01.T01:CDS | 50.0% | |
| ! | TTTGTTGTGGCGAAGGGTTG+TGG | + | Chr1:18615916-18615935 | MsG0180001272.01.T01:CDS | 50.0% |
| !!! | CCTGAGTTTTGTTGTGGCGA+AGG | + | Chr1:18615909-18615928 | MsG0180001272.01.T01:CDS | 50.0% |
| TGTTGTGGCGAAGGGTTGTG+GGG | + | Chr1:18615918-18615937 | MsG0180001272.01.T01:CDS | 55.0% | |
| GTGGCGAAGGGTTGTGGGGA+AGG | + | Chr1:18615922-18615941 | MsG0180001272.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 18615726 | 18616163 | 18615726 | ID=MsG0180001272.01;Name=MsG0180001272.01 |
| Chr1 | mRNA | 18615726 | 18616163 | 18615726 | ID=MsG0180001272.01.T01;Parent=MsG0180001272.01;Name=MsG0180001272.01.T01;_AED=0.41;_eAED=0.41;_QI=0|0|0|1|1|1|2|0|109 |
| Chr1 | exon | 18615726 | 18615943 | 18615726 | ID=MsG0180001272.01.T01:exon:32463;Parent=MsG0180001272.01.T01 |
| Chr1 | exon | 18616052 | 18616163 | 18616052 | ID=MsG0180001272.01.T01:exon:32464;Parent=MsG0180001272.01.T01 |
| Chr1 | CDS | 18615726 | 18615943 | 18615726 | ID=MsG0180001272.01.T01:cds;Parent=MsG0180001272.01.T01 |
| Chr1 | CDS | 18616052 | 18616163 | 18616052 | ID=MsG0180001272.01.T01:cds;Parent=MsG0180001272.01.T01 |
| Gene Sequence |
| Protein sequence |
