Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022167.01.T01 | XP_003607890.1 | 87.97 | 133 | 5 | 1 | 1 | 133 | 1 | 122 | 1.29E-77 | 236 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022167.01.T01 | O82179 | 71.429 | 133 | 28 | 1 | 1 | 133 | 1 | 123 | 1.77E-63 | 193 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022167.01.T01 | Q2MGQ2 | 87.970 | 133 | 5 | 1 | 1 | 133 | 1 | 122 | 6.17e-78 | 236 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047814.01 | MsG0480022167.01 | 0.846788 | 1.742520e-59 | 3.033494e-56 |
| MsG0080048150.01 | MsG0480022167.01 | 0.862416 | 5.148840e-64 | 1.506792e-60 |
| MsG0080049059.01 | MsG0480022167.01 | 0.811994 | 5.300209e-51 | 3.329641e-48 |
| MsG0180000001.01 | MsG0480022167.01 | 0.830877 | 2.292444e-55 | 2.446672e-52 |
| MsG0180000360.01 | MsG0480022167.01 | 0.826131 | 3.210441e-54 | 2.984569e-51 |
| MsG0180000373.01 | MsG0480022167.01 | 0.817367 | 3.421041e-52 | 2.486537e-49 |
| MsG0180001237.01 | MsG0480022167.01 | 0.804920 | 1.713317e-49 | 8.945316e-47 |
| MsG0180001272.01 | MsG0480022167.01 | 0.801315 | 9.540794e-49 | 4.542274e-46 |
| MsG0180003337.01 | MsG0480022167.01 | 0.832073 | 1.163955e-55 | 1.287026e-52 |
| MsG0180003537.01 | MsG0480022167.01 | 0.806142 | 9.496704e-50 | 5.115797e-47 |
| MsG0180004027.01 | MsG0480022167.01 | 0.824880 | 6.350923e-54 | 5.697960e-51 |
| MsG0180004510.01 | MsG0480022167.01 | 0.830970 | 2.175801e-55 | 2.328590e-52 |
| MsG0180004559.01 | MsG0480022167.01 | 0.806642 | 7.450905e-50 | 4.065002e-47 |
| MsG0180004754.01 | MsG0480022167.01 | 0.805099 | 1.572054e-49 | 8.244829e-47 |
| MsG0180004989.01 | MsG0480022167.01 | 0.827021 | 1.968781e-54 | 1.877599e-51 |
| MsG0180005388.01 | MsG0480022167.01 | -0.843510 | 1.340533e-58 | 2.102143e-55 |
| MsG0180005514.01 | MsG0480022167.01 | 0.842811 | 2.058603e-58 | 3.157699e-55 |
| MsG0180005667.01 | MsG0480022167.01 | 0.815829 | 7.565885e-52 | 5.272288e-49 |
| MsG0180005981.01 | MsG0480022167.01 | 0.807040 | 6.138169e-50 | 3.383650e-47 |
| MsG0180006026.01 | MsG0480022167.01 | 0.809587 | 1.758088e-50 | 1.036368e-47 |
| MsG0180006235.01 | MsG0480022167.01 | 0.825725 | 4.007614e-54 | 3.682918e-51 |
| MsG0280006420.01 | MsG0480022167.01 | 0.827758 | 1.310857e-54 | 1.277263e-51 |
| MsG0280010633.01 | MsG0480022167.01 | 0.841777 | 3.868052e-58 | 5.743647e-55 |
| MsG0280010794.01 | MsG0480022167.01 | 0.815033 | 1.137194e-51 | 7.754110e-49 |
| MsG0280010868.01 | MsG0480022167.01 | 0.816099 | 6.584128e-52 | 4.622589e-49 |
| MsG0280011399.01 | MsG0480022167.01 | 0.813661 | 2.286417e-51 | 1.502189e-48 |
| MsG0380014754.01 | MsG0480022167.01 | 0.853509 | 2.280340e-61 | 4.939107e-58 |
| MsG0480018641.01 | MsG0480022167.01 | 0.835849 | 1.320807e-56 | 1.635107e-53 |
| MsG0480019588.01 | MsG0480022167.01 | 0.804230 | 2.386784e-49 | 1.224013e-46 |
| MsG0480019635.01 | MsG0480022167.01 | 0.851271 | 9.891764e-61 | 1.991387e-57 |
| MsG0480020263.01 | MsG0480022167.01 | 0.873968 | 9.742794e-68 | 4.309401e-64 |
| MsG0480021156.01 | MsG0480022167.01 | 0.817486 | 3.216843e-52 | 2.345803e-49 |
| MsG0480021225.01 | MsG0480022167.01 | 0.849739 | 2.664161e-60 | 5.100191e-57 |
| MsG0480021812.01 | MsG0480022167.01 | 0.854780 | 9.801845e-62 | 2.214088e-58 |
| MsG0480022167.01 | MsG0480022325.01 | 0.869411 | 3.160289e-66 | 1.184986e-62 |
| MsG0480022167.01 | MsG0480022407.01 | 0.839252 | 1.771130e-57 | 2.431807e-54 |
| MsG0480022167.01 | MsG0480022620.01 | 0.844452 | 7.494961e-59 | 1.210464e-55 |
| MsG0480022167.01 | MsG0480023013.01 | 0.847116 | 1.417341e-59 | 2.493568e-56 |
| MsG0480022167.01 | MsG0480023105.01 | 0.825633 | 4.213944e-54 | 3.862397e-51 |
| MsG0480022167.01 | MsG0480023195.01 | 0.823226 | 1.552654e-53 | 1.328865e-50 |
| MsG0480022167.01 | MsG0480023324.01 | 0.857985 | 1.123720e-62 | 2.824862e-59 |
| MsG0480022167.01 | MsG0480023541.01 | 0.823675 | 1.219233e-53 | 1.056956e-50 |
| MsG0480022167.01 | MsG0480023869.01 | -0.802088 | 6.623423e-49 | 3.215659e-46 |
| MsG0480022167.01 | MsG0480023874.01 | 0.805032 | 1.623306e-49 | 8.498883e-47 |
| MsG0480022167.01 | MsG0580024035.01 | 0.807478 | 4.956434e-50 | 2.763542e-47 |
| MsG0480022167.01 | MsG0580024109.01 | 0.827873 | 1.229669e-54 | 1.202272e-51 |
| MsG0480022167.01 | MsG0580024388.01 | 0.816231 | 6.151002e-52 | 4.333896e-49 |
| MsG0480022167.01 | MsG0580024478.01 | 0.895940 | 5.942398e-76 | 6.290572e-72 |
| MsG0480022167.01 | MsG0580024618.01 | 0.832313 | 1.014913e-55 | 1.130049e-52 |
| MsG0480022167.01 | MsG0580025352.01 | 0.825240 | 5.220664e-54 | 4.731961e-51 |
| MsG0480022167.01 | MsG0580025385.01 | 0.863822 | 1.892020e-64 | 5.813178e-61 |
| MsG0480022167.01 | MsG0580025766.01 | 0.825160 | 5.452445e-54 | 4.931008e-51 |
| MsG0480022167.01 | MsG0580026157.01 | 0.838405 | 2.931957e-57 | 3.923100e-54 |
| MsG0480022167.01 | MsG0580027131.01 | 0.800391 | 1.473198e-48 | 6.851505e-46 |
| MsG0480022167.01 | MsG0580027413.01 | 0.873667 | 1.231420e-67 | 5.387918e-64 |
| MsG0480022167.01 | MsG0580028060.01 | 0.862792 | 3.942132e-64 | 1.169037e-60 |
| MsG0480022167.01 | MsG0580028330.01 | 0.879067 | 1.686515e-69 | 9.016152e-66 |
| MsG0480022167.01 | MsG0580028449.01 | 0.818173 | 2.250040e-52 | 1.671978e-49 |
| MsG0480022167.01 | MsG0580028924.01 | 0.807217 | 5.629735e-50 | 3.117731e-47 |
| MsG0480022167.01 | MsG0580029023.01 | 0.817079 | 3.972467e-52 | 2.864581e-49 |
| MsG0480022167.01 | MsG0580030182.01 | 0.818817 | 1.607809e-52 | 1.216138e-49 |
| MsG0480022167.01 | MsG0680031178.01 | 0.816189 | 6.288077e-52 | 4.425418e-49 |
| MsG0480022167.01 | MsG0680031549.01 | 0.807539 | 4.812171e-50 | 2.687267e-47 |
| MsG0480022167.01 | MsG0680031616.01 | 0.813765 | 2.168850e-51 | 1.428914e-48 |
| MsG0480022167.01 | MsG0680033623.01 | 0.821818 | 3.297680e-53 | 2.711905e-50 |
| MsG0480022167.01 | MsG0680034598.01 | 0.838582 | 2.639937e-57 | 3.551657e-54 |
| MsG0480022167.01 | MsG0780036133.01 | 0.835019 | 2.141069e-56 | 2.585342e-53 |
| MsG0480022167.01 | MsG0780038373.01 | 0.800394 | 1.471306e-48 | 6.843199e-46 |
| MsG0480022167.01 | MsG0780038790.01 | 0.806289 | 8.843519e-50 | 4.781850e-47 |
| MsG0480022167.01 | MsG0780038837.01 | 0.841752 | 3.927093e-58 | 5.826735e-55 |
| MsG0480022167.01 | MsG0780039137.01 | 0.813929 | 1.995468e-51 | 1.320547e-48 |
| MsG0480022167.01 | MsG0780039462.01 | -0.826745 | 2.291448e-54 | 2.167829e-51 |
| MsG0480022167.01 | MsG0780039533.01 | 0.800403 | 1.464916e-48 | 6.815144e-46 |
| MsG0480022167.01 | MsG0780039739.01 | 0.844050 | 9.608360e-59 | 1.532667e-55 |
| MsG0480022167.01 | MsG0780040030.01 | 0.822719 | 2.038109e-53 | 1.719638e-50 |
| MsG0480022167.01 | MsG0780040364.01 | 0.802972 | 4.353190e-49 | 2.161491e-46 |
| MsG0480022167.01 | MsG0780040482.01 | 0.885005 | 1.180048e-71 | 7.964151e-68 |
| MsG0480022167.01 | MsG0780040483.01 | 0.840438 | 8.696605e-58 | 1.238734e-54 |
| MsG0480022167.01 | MsG0780040543.01 | 0.834083 | 3.680125e-56 | 4.320783e-53 |
| MsG0480022167.01 | MsG0780040564.01 | 0.832397 | 9.679310e-56 | 1.080551e-52 |
| MsG0480022167.01 | MsG0780040688.01 | 0.811845 | 5.711094e-51 | 3.573333e-48 |
| MsG0480022167.01 | MsG0780040880.01 | 0.812581 | 3.944166e-51 | 2.517281e-48 |
| MsG0480022167.01 | MsG0780041363.01 | 0.822527 | 2.258106e-53 | 1.894963e-50 |
| MsG0480022167.01 | MsG0780041565.01 | 0.842045 | 3.285611e-58 | 4.920150e-55 |
| MsG0480022167.01 | MsG0780041641.01 | 0.830243 | 3.277604e-55 | 3.433931e-52 |
| MsG0480022167.01 | MsG0880041883.01 | 0.833936 | 4.005555e-56 | 4.682745e-53 |
| MsG0480022167.01 | MsG0880042046.01 | 0.853074 | 3.037979e-61 | 6.483955e-58 |
| MsG0480022167.01 | MsG0880042349.01 | 0.864184 | 1.459213e-64 | 4.540999e-61 |
| MsG0480022167.01 | MsG0880042552.01 | 0.836582 | 8.601696e-57 | 1.088587e-53 |
| MsG0480022167.01 | MsG0880042583.01 | 0.815159 | 1.066589e-51 | 7.297098e-49 |
| MsG0480022167.01 | MsG0880043261.01 | 0.836503 | 9.008660e-57 | 1.137471e-53 |
| MsG0480022167.01 | MsG0880043297.01 | 0.823212 | 1.563875e-53 | 1.337959e-50 |
| MsG0480022167.01 | MsG0880043959.01 | 0.805558 | 1.259891e-49 | 6.685794e-47 |
| MsG0480022167.01 | MsG0880043961.01 | 0.823653 | 1.233338e-53 | 1.068545e-50 |
| MsG0480022167.01 | MsG0880043983.01 | 0.829508 | 4.950730e-55 | 5.077134e-52 |
| MsG0480022167.01 | MsG0880043989.01 | 0.844903 | 5.665391e-59 | 9.281143e-56 |
| MsG0480022167.01 | MsG0880044792.01 | 0.808178 | 3.517997e-50 | 1.997899e-47 |
| MsG0480022167.01 | MsG0880046086.01 | 0.827706 | 1.349122e-54 | 1.312595e-51 |
| MsG0480022167.01 | MsG0880046165.01 | 0.808652 | 2.787887e-50 | 1.602891e-47 |
| MsG0480022167.01 | MsG0880046445.01 | 0.845684 | 3.482951e-59 | 5.850956e-56 |
| MsG0480022167.01 | MsG0880046762.01 | 0.828947 | 6.772277e-55 | 6.831328e-52 |
| MsG0480022167.01 | MsG0880046922.01 | 0.822376 | 2.448314e-53 | 2.045779e-50 |
| MsG0480022167.01 | MsG0880046933.01 | 0.813369 | 2.651013e-51 | 1.728006e-48 |
| MsG0480022167.01 | MsG0880047012.01 | 0.824663 | 7.142977e-54 | 6.369032e-51 |
| MsG0480022167.01 | MsG0880047466.01 | 0.822298 | 2.552680e-53 | 2.128149e-50 |
| MsG0380015548.01 | MsG0480022167.01 | 0.828019 | 1.134619e-54 | 1.114144e-51 |
| MsG0380015641.01 | MsG0480022167.01 | 0.819288 | 1.256458e-52 | 9.627899e-50 |
| MsG0380015793.01 | MsG0480022167.01 | -0.841272 | 5.254943e-58 | 7.680915e-55 |
| MsG0380016389.01 | MsG0480022167.01 | 0.839585 | 1.450695e-57 | 2.012362e-54 |
| MsG0380016449.01 | MsG0480022167.01 | 0.824267 | 8.855902e-54 | 7.805412e-51 |
| MsG0380016994.01 | MsG0480022167.01 | 0.807061 | 6.074817e-50 | 3.350558e-47 |
| MsG0380017049.01 | MsG0480022167.01 | 0.801877 | 7.316583e-49 | 3.533411e-46 |
| MsG0380017231.01 | MsG0480022167.01 | 0.865532 | 5.514770e-65 | 1.800354e-61 |
| MsG0380017240.01 | MsG0480022167.01 | 0.862482 | 4.910826e-64 | 1.440561e-60 |
| MsG0380017998.01 | MsG0480022167.01 | 0.805911 | 1.061840e-49 | 5.686540e-47 |
| MsG0380018007.01 | MsG0480022167.01 | 0.848395 | 6.297763e-60 | 1.154120e-56 |
| MsG0380018060.01 | MsG0480022167.01 | 0.821274 | 4.404571e-53 | 3.566870e-50 |
| MsG0480018256.01 | MsG0480022167.01 | 0.840851 | 6.777259e-58 | 9.778442e-55 |
| MsG0280006884.01 | MsG0480022167.01 | 0.800856 | 1.184094e-48 | 5.572520e-46 |
| MsG0280007196.01 | MsG0480022167.01 | 0.820995 | 5.108941e-53 | 4.105887e-50 |
| MsG0280007419.01 | MsG0480022167.01 | 0.828196 | 1.028199e-54 | 1.014845e-51 |
| MsG0280007609.01 | MsG0480022167.01 | 0.836392 | 9.616045e-57 | 1.210152e-53 |
| MsG0280007706.01 | MsG0480022167.01 | 0.800819 | 1.204885e-48 | 5.664684e-46 |
| MsG0280007790.01 | MsG0480022167.01 | 0.848986 | 4.319563e-60 | 8.069594e-57 |
| MsG0280007876.01 | MsG0480022167.01 | 0.870147 | 1.816872e-66 | 6.993831e-63 |
| MsG0280007983.01 | MsG0480022167.01 | 0.873262 | 1.684851e-67 | 7.265607e-64 |
| MsG0280008280.01 | MsG0480022167.01 | 0.827272 | 1.714136e-54 | 1.646954e-51 |
| MsG0280008327.01 | MsG0480022167.01 | 0.854091 | 1.550712e-61 | 3.424460e-58 |
| MsG0280008679.01 | MsG0480022167.01 | 0.837872 | 4.023316e-57 | 5.296778e-54 |
| MsG0280008853.01 | MsG0480022167.01 | 0.829192 | 5.904879e-55 | 6.000600e-52 |
| MsG0280008885.01 | MsG0480022167.01 | 0.803967 | 2.707524e-49 | 1.379233e-46 |
| MsG0280009241.01 | MsG0480022167.01 | 0.856860 | 2.417563e-62 | 5.850282e-59 |
| MsG0280009255.01 | MsG0480022167.01 | 0.819094 | 1.390927e-52 | 1.060201e-49 |
| MsG0280009514.01 | MsG0480022167.01 | 0.818589 | 1.811486e-52 | 1.361579e-49 |
| MsG0280010002.01 | MsG0480022167.01 | 0.869169 | 3.787123e-66 | 1.407480e-62 |
| MsG0280010296.01 | MsG0480022167.01 | 0.803015 | 4.264468e-49 | 2.119741e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022167.01.T01 | MTR_4g084110 | 87.970 | 133 | 5 | 1 | 1 | 133 | 1 | 122 | 1.56e-81 | 236 |
| MsG0480022167.01.T01 | MTR_5g014260 | 66.667 | 105 | 32 | 1 | 32 | 133 | 29 | 133 | 8.11e-47 | 149 |
| MsG0480022167.01.T01 | MTR_5g014260 | 65.979 | 97 | 30 | 1 | 32 | 125 | 29 | 125 | 2.84e-41 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022167.01.T01 | AT2G35120 | 71.429 | 133 | 28 | 1 | 1 | 133 | 1 | 123 | 1.81e-64 | 193 |
| MsG0480022167.01.T01 | AT1G32470 | 65.714 | 105 | 33 | 1 | 32 | 133 | 29 | 133 | 1.38e-43 | 141 |
| MsG0480022167.01.T01 | AT2G35370 | 56.391 | 133 | 57 | 1 | 1 | 133 | 1 | 132 | 7.16e-43 | 139 |
Find 45 sgRNAs with CRISPR-Local
Find 117 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACATCAACTCTCCTGTTTC+AGG | 0.209182 | 4:-69229468 | MsG0480022167.01.T01:CDS |
| CAGTGCGAACTTGACAAATT+TGG | 0.221353 | 4:-69229230 | MsG0480022167.01.T01:three_prime_UTR |
| TTCACTGCCGTTCCCACTTC+TGG | 0.241034 | 4:+69229537 | None:intergenic |
| AGAAGTTCTATGAATTTAAT+AGG | 0.244254 | 4:-69228863 | MsG0480022167.01.T01:three_prime_UTR |
| AACTCAAGCCCCTATGAAAA+TGG | 0.268193 | 4:-69229279 | MsG0480022167.01.T01:CDS |
| ACAGAGAAAAGATGGCTTGC+AGG | 0.340068 | 4:-69230980 | None:intergenic |
| TGATCATGCCCAAGATCATT+TGG | 0.340864 | 4:-69229583 | MsG0480022167.01.T01:CDS |
| AGATGGCTTGCAGGCAGTTA+TGG | 0.342329 | 4:-69230971 | MsG0480022167.01.T01:CDS |
| GTGAAACAAGGAGATGGTTT+TGG | 0.366878 | 4:-69229519 | MsG0480022167.01.T01:CDS |
| ATCTTCTTCTTCACAGAACT+TGG | 0.368009 | 4:+69229192 | None:intergenic |
| CAACATCACCCAAATGATCT+TGG | 0.387609 | 4:+69229574 | None:intergenic |
| GATGGCTTGCAGGCAGTTAT+GGG | 0.405390 | 4:-69230970 | MsG0480022167.01.T01:CDS |
| GCTCAGCAACTCTCCTGCTT+TGG | 0.435121 | 4:-69229421 | MsG0480022167.01.T01:intron |
| CCTGAGGTATGATGCGGCTC+TGG | 0.441316 | 4:+69230944 | None:intergenic |
| TTGGTATAGATGATGTCAAT+TGG | 0.476485 | 4:-69229127 | MsG0480022167.01.T01:three_prime_UTR |
| TGAATGTCGTATGACAAAAC+TGG | 0.478972 | 4:+69228924 | None:intergenic |
| GATCATGCCCAAGATCATTT+GGG | 0.482133 | 4:-69229582 | MsG0480022167.01.T01:CDS |
| TGGGAAGTACTGAAAGATAT+CGG | 0.488163 | 4:+69229150 | None:intergenic |
| GCAATAAAACTCACCAAAGC+AGG | 0.490891 | 4:+69229408 | None:intergenic |
| CATGAATGGGTGAAAGTTGA+CGG | 0.492157 | 4:-69229630 | MsG0480022167.01.T01:CDS |
| AACATCACCCAAATGATCTT+GGG | 0.497081 | 4:+69229575 | None:intergenic |
| CCAGAGCCGCATCATACCTC+AGG | 0.521213 | 4:-69230944 | MsG0480022167.01.T01:CDS |
| AGTATGCTGATTCTCATGAA+TGG | 0.523325 | 4:-69229644 | MsG0480022167.01.T01:CDS |
| TGCGCTAGCTTCTGATGCAG+AGG | 0.534256 | 4:-69229046 | MsG0480022167.01.T01:three_prime_UTR |
| TTGACATCATCTATACCAAT+GGG | 0.544257 | 4:+69229131 | None:intergenic |
| TATCTTTCAGTACTTCCCAT+TGG | 0.548271 | 4:-69229146 | MsG0480022167.01.T01:three_prime_UTR |
| GCGCTAGCTTCTGATGCAGA+GGG | 0.548373 | 4:-69229045 | MsG0480022167.01.T01:three_prime_UTR |
| TTCCATCTTAGTTGAAAACA+AGG | 0.549310 | 4:+69228887 | None:intergenic |
| GTATGCTGATTCTCATGAAT+GGG | 0.551627 | 4:-69229643 | MsG0480022167.01.T01:CDS |
| GACGGAAAATCTGCAACCAT+TGG | 0.558598 | 4:-69229612 | MsG0480022167.01.T01:CDS |
| ATTGACATCATCTATACCAA+TGG | 0.564510 | 4:+69229130 | None:intergenic |
| TTTCACAGAACCTTCTCTAA+CGG | 0.580128 | 4:-69230912 | MsG0480022167.01.T01:CDS |
| TCCATCTTAGTTGAAAACAA+GGG | 0.618905 | 4:+69228888 | None:intergenic |
| TGAATGAATACCGTTAGAGA+AGG | 0.619094 | 4:+69230902 | None:intergenic |
| TTGGTGCAGTTGAAAGTGTG+AGG | 0.620032 | 4:-69229500 | MsG0480022167.01.T01:CDS |
| TGAATTGCCAGAAGTGGGAA+CGG | 0.630383 | 4:-69229544 | MsG0480022167.01.T01:CDS |
| GTGGGAACGGCAGTGAAACA+AGG | 0.631106 | 4:-69229531 | MsG0480022167.01.T01:CDS |
| ACGGCAGTGAAACAAGGAGA+TGG | 0.634473 | 4:-69229525 | MsG0480022167.01.T01:CDS |
| ATCGGCAAGACTATCAATGA+CGG | 0.637348 | 4:+69229168 | None:intergenic |
| TGGTGCAGTTGAAAGTGTGA+GGG | 0.639998 | 4:-69229499 | MsG0480022167.01.T01:CDS |
| GGCATGATCAGTTATGCCAA+TGG | 0.640158 | 4:+69229596 | None:intergenic |
| GTATGTTGAATTGCCAGAAG+TGG | 0.646780 | 4:-69229550 | MsG0480022167.01.T01:CDS |
| TGTGAAAGACAGAGATCCTG+AGG | 0.655630 | 4:+69230928 | None:intergenic |
| TATGTTGAATTGCCAGAAGT+GGG | 0.663199 | 4:-69229549 | MsG0480022167.01.T01:CDS |
| AGAGATCCTGAGGTATGATG+CGG | 0.690860 | 4:+69230938 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAAAGTTAATTATGAATA+AGG | + | Chr4:69230417-69230436 | None:intergenic | 10.0% |
| !! | ATATAATAAAATAAAAAGCG+AGG | + | Chr4:69229001-69229020 | None:intergenic | 15.0% |
| !! | TTAAAATTCAATATGCAAAT+TGG | + | Chr4:69229889-69229908 | None:intergenic | 15.0% |
| !! | TTGTTAACAAAAAAACAAAA+TGG | + | Chr4:69229232-69229251 | None:intergenic | 15.0% |
| !!! | AGATTTTATTGAGATATTAA+CGG | + | Chr4:69229696-69229715 | None:intergenic | 15.0% |
| !!! | TTTTAATATCTTGAAAGTTT+TGG | - | Chr4:69229903-69229922 | MsG0480022167.01.T01:intron | 15.0% |
| !! | AAAAATGTAACAAACAACAT+AGG | + | Chr4:69229330-69229349 | None:intergenic | 20.0% |
| !! | AAAATTTATAATGTGTAGGA+TGG | - | Chr4:69229844-69229863 | MsG0480022167.01.T01:intron | 20.0% |
| !! | AACCAAAATTTATAATGTGT+AGG | - | Chr4:69229840-69229859 | MsG0480022167.01.T01:intron | 20.0% |
| !! | AGAAGTTCTATGAATTTAAT+AGG | - | Chr4:69230937-69230956 | MsG0480022167.01.T01:CDS | 20.0% |
| !! | AGATCAAGTTCAAAATAAAT+TGG | + | Chr4:69229101-69229120 | None:intergenic | 20.0% |
| !! | ATATGTAAGTAACATATACT+AGG | - | Chr4:69229251-69229270 | MsG0480022167.01.T01:three_prime_UTR | 20.0% |
| !!! | AAATGTTTGAATAGCTTTAT+AGG | + | Chr4:69229596-69229615 | None:intergenic | 20.0% |
| !!! | AATTATCATCCATTTTCATA+GGG | + | Chr4:69230533-69230552 | None:intergenic | 20.0% |
| !!! | ATCCTACACATTATAAATTT+TGG | + | Chr4:69229845-69229864 | None:intergenic | 20.0% |
| !!! | ATTTTGTTCTAACTATTATC+TGG | - | Chr4:69229725-69229744 | MsG0480022167.01.T01:intron | 20.0% |
| !!! | GATTTTATTGAGATATTAAC+GGG | + | Chr4:69229695-69229714 | None:intergenic | 20.0% |
| !!! | TAATTATCATCCATTTTCAT+AGG | + | Chr4:69230534-69230553 | None:intergenic | 20.0% |
| ! | ATAACTCAGTCAAAAGAAAT+TGG | + | Chr4:69229560-69229579 | None:intergenic | 25.0% |
| ! | ATAGGAAACATAAAAACACT+TGG | + | Chr4:69230484-69230503 | None:intergenic | 25.0% |
| ! | TATTATCTGGTACATCATAT+TGG | - | Chr4:69229738-69229757 | MsG0480022167.01.T01:intron | 25.0% |
| ! | TCAAGTTCAAAATAAATTGG+AGG | + | Chr4:69229098-69229117 | None:intergenic | 25.0% |
| !! | AAAAATGAGATTGATAACCT+GGG | - | Chr4:69230803-69230822 | MsG0480022167.01.T01:intron | 25.0% |
| !! | AGAATATTCCATTTCCATTT+TGG | + | Chr4:69229284-69229303 | None:intergenic | 25.0% |
| !! | ATTATCATCCATTTTCATAG+GGG | + | Chr4:69230532-69230551 | None:intergenic | 25.0% |
| !! | TTTTTATTATCAAACTTCCC+AGG | + | Chr4:69230823-69230842 | None:intergenic | 25.0% |
| !!! | GAATAGCTTTATAGGTAAAT+TGG | + | Chr4:69229588-69229607 | None:intergenic | 25.0% |
| AATAATCCAAATTACTCTCG+AGG | - | Chr4:69229619-69229638 | MsG0480022167.01.T01:CDS | 30.0% | |
| AATTGAAAGAATAGATCGTC+CGG | + | Chr4:69229183-69229202 | None:intergenic | 30.0% | |
| ATTGACATCATCTATACCAA+TGG | + | Chr4:69230673-69230692 | None:intergenic | 30.0% | |
| ATTGCCTTTATATACGTTAG+TGG | - | Chr4:69229038-69229057 | MsG0480022167.01.T01:three_prime_UTR | 30.0% | |
| GCTAAATTCCAAAATGGAAA+TGG | - | Chr4:69229273-69229292 | MsG0480022167.01.T01:exon | 30.0% | |
| GCTTACAAATGAACATGATA+TGG | + | Chr4:69229804-69229823 | None:intergenic | 30.0% | |
| GTGCATATTTCGAAATTAAG+TGG | - | Chr4:69229406-69229425 | MsG0480022167.01.T01:intron | 30.0% | |
| TAATGATAATCATCATCTGC+AGG | - | Chr4:69229984-69230003 | MsG0480022167.01.T01:intron | 30.0% | |
| TACATGATAATCATCATCTG+CGG | - | Chr4:69229960-69229979 | MsG0480022167.01.T01:intron | 30.0% | |
| TACTAGGCTAAATTCCAAAA+TGG | - | Chr4:69229267-69229286 | MsG0480022167.01.T01:exon | 30.0% | |
| TCCATCTTAGTTGAAAACAA+GGG | + | Chr4:69230915-69230934 | None:intergenic | 30.0% | |
| TTAAAACTTACGTGAACGAT+GGG | - | Chr4:69229772-69229791 | MsG0480022167.01.T01:intron | 30.0% | |
| TTCCATCTTAGTTGAAAACA+AGG | + | Chr4:69230916-69230935 | None:intergenic | 30.0% | |
| TTGACATCATCTATACCAAT+GGG | + | Chr4:69230672-69230691 | None:intergenic | 30.0% | |
| TTGCCTTTATATACGTTAGT+GGG | - | Chr4:69229039-69229058 | MsG0480022167.01.T01:three_prime_UTR | 30.0% | |
| TTGGTATAGATGATGTCAAT+TGG | - | Chr4:69230673-69230692 | MsG0480022167.01.T01:intron | 30.0% | |
| ! | ATTGAAACCAACATTTTGCA+AGG | - | Chr4:69230084-69230103 | MsG0480022167.01.T01:intron | 30.0% |
| ! | GAAAAATGAGATTGATAACC+TGG | - | Chr4:69230802-69230821 | MsG0480022167.01.T01:intron | 30.0% |
| ! | TTTTGAACTTGATCTGTATG+TGG | - | Chr4:69229106-69229125 | MsG0480022167.01.T01:three_prime_UTR | 30.0% |
| !! | AATTTAGCTTGTTAGCACTT+TGG | - | Chr4:69229482-69229501 | MsG0480022167.01.T01:CDS | 30.0% |
| !! | ATCTCATTTTTCCATTGCAA+AGG | + | Chr4:69230794-69230813 | None:intergenic | 30.0% |
| !! | ATTTAGCTTGTTAGCACTTT+GGG | - | Chr4:69229483-69229502 | MsG0480022167.01.T01:CDS | 30.0% |
| !! | TAACAAACTGTTGGTTTGTT+TGG | - | Chr4:69229512-69229531 | MsG0480022167.01.T01:CDS | 30.0% |
| !! | TAACGGGTTAATTGATTTCA+AGG | + | Chr4:69229679-69229698 | None:intergenic | 30.0% |
| !! | TCTCATTTTTCCATTGCAAA+GGG | + | Chr4:69230793-69230812 | None:intergenic | 30.0% |
| !! | TTTCCTATTTTTCTTCGTGT+AGG | - | Chr4:69230496-69230515 | MsG0480022167.01.T01:intron | 30.0% |
| AACATCACCCAAATGATCTT+GGG | + | Chr4:69230228-69230247 | None:intergenic | 35.0% | |
| ATAAATTGGAGGTGACTGAA+TGG | + | Chr4:69229087-69229106 | None:intergenic | 35.0% | |
| ATCTTCTTCTTCACAGAACT+TGG | + | Chr4:69230611-69230630 | None:intergenic | 35.0% | |
| ATGTTTCCTCGAGAGTAATT+TGG | + | Chr4:69229628-69229647 | None:intergenic | 35.0% | |
| CATACAACCTTGCAAAATGT+TGG | + | Chr4:69230094-69230113 | None:intergenic | 35.0% | |
| CCATTGAACAAGTAGCATAT+TGG | - | Chr4:69230035-69230054 | MsG0480022167.01.T01:intron | 35.0% | |
| CTTCCCACTAACGTATATAA+AGG | + | Chr4:69229045-69229064 | None:intergenic | 35.0% | |
| CTTTATATACGTTAGTGGGA+AGG | - | Chr4:69229043-69229062 | MsG0480022167.01.T01:three_prime_UTR | 35.0% | |
| GTTAAAACTTACGTGAACGA+TGG | - | Chr4:69229771-69229790 | MsG0480022167.01.T01:intron | 35.0% | |
| TATCTTTCAGTACTTCCCAT+TGG | - | Chr4:69230654-69230673 | MsG0480022167.01.T01:intron | 35.0% | |
| TATGTTGAATTGCCAGAAGT+GGG | - | Chr4:69230251-69230270 | MsG0480022167.01.T01:intron | 35.0% | |
| TGAATGAATACCGTTAGAGA+AGG | + | Chr4:69228901-69228920 | None:intergenic | 35.0% | |
| TGAATGTCGTATGACAAAAC+TGG | + | Chr4:69230879-69230898 | None:intergenic | 35.0% | |
| TGACCTACACGAAGAAAAAT+AGG | + | Chr4:69230502-69230521 | None:intergenic | 35.0% | |
| TTTCACAGAACCTTCTCTAA+CGG | - | Chr4:69228888-69228907 | MsG0480022167.01.T01:three_prime_UTR | 35.0% | |
| ! | CCAATATGCTACTTGTTCAA+TGG | + | Chr4:69230038-69230057 | None:intergenic | 35.0% |
| !! | AGTATGCTGATTCTCATGAA+TGG | - | Chr4:69230156-69230175 | MsG0480022167.01.T01:intron | 35.0% |
| !! | CTCATTTTTCCATTGCAAAG+GGG | + | Chr4:69230792-69230811 | None:intergenic | 35.0% |
| !! | GTATGCTGATTCTCATGAAT+GGG | - | Chr4:69230157-69230176 | MsG0480022167.01.T01:intron | 35.0% |
| !! | TCAATCACTTTTCCTGAAAC+AGG | + | Chr4:69230347-69230366 | None:intergenic | 35.0% |
| !! | TCATTTTTCCATTGCAAAGG+GGG | + | Chr4:69230791-69230810 | None:intergenic | 35.0% |
| !! | TGGGAAGTACTGAAAGATAT+CGG | + | Chr4:69230653-69230672 | None:intergenic | 35.0% |
| !! | TTTAGCTTGTTAGCACTTTG+GGG | - | Chr4:69229484-69229503 | MsG0480022167.01.T01:CDS | 35.0% |
| AACTCAAGCCCCTATGAAAA+TGG | - | Chr4:69230521-69230540 | MsG0480022167.01.T01:intron | 40.0% | |
| ATCGGCAAGACTATCAATGA+CGG | + | Chr4:69230635-69230654 | None:intergenic | 40.0% | |
| CAACATCACCCAAATGATCT+TGG | + | Chr4:69230229-69230248 | None:intergenic | 40.0% | |
| CAAGATCCAGATTCCAGATA+TGG | + | Chr4:69228976-69228995 | None:intergenic | 40.0% | |
| CAGTGCGAACTTGACAAATT+TGG | - | Chr4:69230570-69230589 | MsG0480022167.01.T01:intron | 40.0% | |
| CATGAATGGGTGAAAGTTGA+CGG | - | Chr4:69230170-69230189 | MsG0480022167.01.T01:intron | 40.0% | |
| GATCATGCCCAAGATCATTT+GGG | - | Chr4:69230218-69230237 | MsG0480022167.01.T01:intron | 40.0% | |
| GCAATAAAACTCACCAAAGC+AGG | + | Chr4:69230395-69230414 | None:intergenic | 40.0% | |
| GGGGGAAAATAACAAACTGT+TGG | - | Chr4:69229503-69229522 | MsG0480022167.01.T01:CDS | 40.0% | |
| GTATGTTGAATTGCCAGAAG+TGG | - | Chr4:69230250-69230269 | MsG0480022167.01.T01:intron | 40.0% | |
| TGAATGGTGTAGTGCACTTT+CGG | + | Chr4:69229071-69229090 | None:intergenic | 40.0% | |
| TGATCATGCCCAAGATCATT+TGG | - | Chr4:69230217-69230236 | MsG0480022167.01.T01:intron | 40.0% | |
| ! | CGGGTTAATTGATTTCAAGG+AGG | + | Chr4:69229676-69229695 | None:intergenic | 40.0% |
| ! | GATATTTTCCCCCTTTGCAA+TGG | - | Chr4:69230780-69230799 | MsG0480022167.01.T01:intron | 40.0% |
| ! | GTGAAACAAGGAGATGGTTT+TGG | - | Chr4:69230281-69230300 | MsG0480022167.01.T01:intron | 40.0% |
| ! | GTTGATCCATATCTGGAATC+TGG | - | Chr4:69228967-69228986 | MsG0480022167.01.T01:three_prime_UTR | 40.0% |
| ! | TGCATGTGTTGATCCATATC+TGG | - | Chr4:69228960-69228979 | MsG0480022167.01.T01:three_prime_UTR | 40.0% |
| !! | CACCAAAAGTTGATATACGG+CGG | + | Chr4:69229160-69229179 | None:intergenic | 40.0% |
| !! | CGGCACCAAAAGTTGATATA+CGG | + | Chr4:69229163-69229182 | None:intergenic | 40.0% |
| !! | CGTATATCAACTTTTGGTGC+CGG | - | Chr4:69229161-69229180 | MsG0480022167.01.T01:three_prime_UTR | 40.0% |
| !! | TTAGCTTGTTAGCACTTTGG+GGG | - | Chr4:69229485-69229504 | MsG0480022167.01.T01:CDS | 40.0% |
| !!! | GCCCTTGTTTTCAACTAAGA+TGG | - | Chr4:69230911-69230930 | MsG0480022167.01.T01:CDS | 40.0% |
| AGAGATCCTGAGGTATGATG+CGG | + | Chr4:69228865-69228884 | None:intergenic | 45.0% | |
| CAGATGAGAAGACAGAGACT+CGG | + | Chr4:69229936-69229955 | None:intergenic | 45.0% | |
| GACATCAACTCTCCTGTTTC+AGG | - | Chr4:69230332-69230351 | MsG0480022167.01.T01:intron | 45.0% | |
| GACGGAAAATCTGCAACCAT+TGG | - | Chr4:69230188-69230207 | MsG0480022167.01.T01:intron | 45.0% | |
| GGCATGATCAGTTATGCCAA+TGG | + | Chr4:69230207-69230226 | None:intergenic | 45.0% | |
| TGAATTGCCAGAAGTGGGAA+CGG | - | Chr4:69230256-69230275 | MsG0480022167.01.T01:intron | 45.0% | |
| TGTGAAAGACAGAGATCCTG+AGG | + | Chr4:69228875-69228894 | None:intergenic | 45.0% | |
| ! | GTCCGCCGTATATCAACTTT+TGG | - | Chr4:69229155-69229174 | MsG0480022167.01.T01:three_prime_UTR | 45.0% |
| ! | TGGTGCAGTTGAAAGTGTGA+GGG | - | Chr4:69230301-69230320 | MsG0480022167.01.T01:intron | 45.0% |
| ! | TTGGTGCAGTTGAAAGTGTG+AGG | - | Chr4:69230300-69230319 | MsG0480022167.01.T01:intron | 45.0% |
| ACGGCAGTGAAACAAGGAGA+TGG | - | Chr4:69230275-69230294 | MsG0480022167.01.T01:intron | 50.0% | |
| CCATAACTGCCTGCAAGCCA+TGG | + | Chr4:69228832-69228851 | None:intergenic | 55.0% | |
| GCTCAGCAACTCTCCTGCTT+TGG | - | Chr4:69230379-69230398 | MsG0480022167.01.T01:intron | 55.0% | |
| GTGGGAACGGCAGTGAAACA+AGG | - | Chr4:69230269-69230288 | MsG0480022167.01.T01:intron | 55.0% | |
| TTCACTGCCGTTCCCACTTC+TGG | + | Chr4:69230266-69230285 | None:intergenic | 55.0% | |
| ! | GCGCTAGCTTCTGATGCAGA+GGG | - | Chr4:69230755-69230774 | MsG0480022167.01.T01:intron | 55.0% |
| ! | TGCGCTAGCTTCTGATGCAG+AGG | - | Chr4:69230754-69230773 | MsG0480022167.01.T01:intron | 55.0% |
| CCAGAGCCGCATCATACCTC+AGG | - | Chr4:69228856-69228875 | MsG0480022167.01.T01:three_prime_UTR | 60.0% | |
| CCTGAGGTATGATGCGGCTC+TGG | + | Chr4:69228859-69228878 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 69228831 | 69230991 | 69228831 | ID=MsG0480022167.01;Name=MsG0480022167.01 |
| Chr4 | mRNA | 69228831 | 69230991 | 69228831 | ID=MsG0480022167.01.T01;Parent=MsG0480022167.01;Name=MsG0480022167.01.T01;_AED=0.32;_eAED=0.33;_QI=0|0.5|0.66|1|0.5|0.66|3|444|133 |
| Chr4 | exon | 69228831 | 69229304 | 69228831 | ID=MsG0480022167.01.T01:exon:18981;Parent=MsG0480022167.01.T01 |
| Chr4 | exon | 69229422 | 69229681 | 69229422 | ID=MsG0480022167.01.T01:exon:18980;Parent=MsG0480022167.01.T01 |
| Chr4 | exon | 69230880 | 69230991 | 69230880 | ID=MsG0480022167.01.T01:exon:18979;Parent=MsG0480022167.01.T01 |
| Chr4 | CDS | 69230880 | 69230991 | 69230880 | ID=MsG0480022167.01.T01:cds;Parent=MsG0480022167.01.T01 |
| Chr4 | CDS | 69229422 | 69229681 | 69229422 | ID=MsG0480022167.01.T01:cds;Parent=MsG0480022167.01.T01 |
| Chr4 | CDS | 69229275 | 69229304 | 69229275 | ID=MsG0480022167.01.T01:cds;Parent=MsG0480022167.01.T01 |
| Chr4 | three_prime_UTR | 69228831 | 69229274 | 69228831 | ID=MsG0480022167.01.T01:three_prime_utr;Parent=MsG0480022167.01.T01 |
| Gene Sequence |
| Protein sequence |
