AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048842.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048842.01.T01 MTR_0493s0030 97.855 373 8 0 1 373 1 373 0.0 745
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048842.01.T01 AT1G74640 70.225 356 100 3 19 372 19 370 0.0 521

Find 44 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCAACTGCAGGCGCCTTTA+AGG 0.104317 contig478end:-22851 None:intergenic
CAATTCTTGCAACAGAATTT+TGG 0.177594 contig478end:+23245 MsG0080048842.01.T01:exon
CCTTCAACTCTTTGCAGATT+TGG 0.235687 contig478end:+23055 MsG0080048842.01.T01:exon
ATGGCTGGTGTCACGTTATC+AGG 0.302525 contig478end:-22949 None:intergenic
TCTTCTAAGAGGCACCTTAA+AGG 0.331435 contig478end:+22818 MsG0080048842.01.T01:exon
TTACCCGCGGCTTTCTTGAC+TGG 0.345149 contig478end:+22999 MsG0080048842.01.T01:exon
AACTCCTCACGGGATGTTAC+AGG 0.348170 contig478end:-23051 None:intergenic
CAACTAAAGGGTCTCCTAAA+AGG 0.373750 contig478end:+23105 MsG0080048842.01.T01:exon
ACCTTAAAGGCGCCTGCAGT+TGG 0.374128 contig478end:+22831 MsG0080048842.01.T01:exon
TTAGTTGATACAACAAATAC+TGG 0.382699 contig478end:-23108 None:intergenic
CCAAATCTGCAAAGAGTTGA+AGG 0.392385 contig478end:-23074 None:intergenic
CCTTAAAGGCGCCTGCAGTT+GGG 0.416753 contig478end:+22832 MsG0080048842.01.T01:exon
GTATTTGTTGTATCAACTAA+AGG 0.441722 contig478end:+23092 MsG0080048842.01.T01:exon
TATTTGTTGTATCAACTAAA+GGG 0.442235 contig478end:+23093 MsG0080048842.01.T01:exon
GCTGAGATGGAAGCTCTTAA+CGG 0.459166 contig478end:+23134 MsG0080048842.01.T01:exon
AGCTCTTCCGCGACTAAAGC+AGG 0.462416 contig478end:-23237 None:intergenic
AGGGTCTCCTAAAAGGTCTA+AGG 0.474040 contig478end:+23112 MsG0080048842.01.T01:exon
TCAACTCTTTGCAGATTTGG+AGG 0.476813 contig478end:+23058 MsG0080048842.01.T01:exon
TAAAAGGTCTAAGGCTGAGA+TGG 0.498747 contig478end:+23121 MsG0080048842.01.T01:exon
CAACTCTTTGCAGATTTGGA+GGG 0.500215 contig478end:+23059 MsG0080048842.01.T01:exon
GAAGCTCTTAACGGAGCTAA+AGG 0.503984 contig478end:+23143 MsG0080048842.01.T01:exon
CATGTGATTTGTACTGCGAT+TGG 0.504645 contig478end:-22915 None:intergenic
TAAAGGAGTGAGCAAGTTTG+TGG 0.516133 contig478end:+23160 MsG0080048842.01.T01:exon
TGCATATCTGCTTTCGACAA+TGG 0.528904 contig478end:-22968 None:intergenic
TGGTGTCACGTTATCAGGAT+TGG 0.532092 contig478end:-22944 None:intergenic
GACAGGTATGGTCTTCTAAG+AGG 0.537611 contig478end:+22807 MsG0080048842.01.T01:intron
TACACCATCCACCCAACTGC+AGG 0.541976 contig478end:-22862 None:intergenic
AGCAAGTTTGTGGAAGTGCC+TGG 0.543593 contig478end:+23170 MsG0080048842.01.T01:exon
GGCGCCTGCAGTTGGGTGGA+TGG 0.552084 contig478end:+22839 MsG0080048842.01.T01:exon
TGGTGCTCTTCTTCCACAAG+AGG 0.557293 contig478end:+23190 MsG0080048842.01.T01:exon
TATCTGCTTTCGACAATGGC+TGG 0.559916 contig478end:-22964 None:intergenic
CACGGGATGTTACAGGGTCT+AGG 0.561594 contig478end:-23044 None:intergenic
GAGTTGAAGGAACTCCTCAC+GGG 0.577784 contig478end:-23061 None:intergenic
TCTTGTGGAAGAAGAGCACC+AGG 0.583551 contig478end:-23207 None:intergenic
AGAGTTGAAGGAACTCCTCA+CGG 0.588545 contig478end:-23062 None:intergenic
AAAGCAGGATACTCCTCTTG+TGG 0.594658 contig478end:-23222 None:intergenic
TAAAGGCGCCTGCAGTTGGG+TGG 0.609424 contig478end:+22835 MsG0080048842.01.T01:exon
AGTCCAGTCAAGAAAGCCGC+GGG 0.612625 contig478end:-23021 None:intergenic
ACTCCTCACGGGATGTTACA+GGG 0.624393 contig478end:-23050 None:intergenic
GAGTCCAGTCAAGAAAGCCG+CGG 0.650624 contig478end:-23022 None:intergenic
GGAGTATCCTGCTTTAGTCG+CGG 0.676120 contig478end:+23211 MsG0080048842.01.T01:exon
AGGAGCACGTTACTTACCCG+CGG 0.688473 contig478end:+22986 MsG0080048842.01.T01:exon
AGACCCTGTAACATCCCGTG+AGG 0.736244 contig478end:+23028 MsG0080048842.01.T01:exon
TATGCATTGACAAAACGACA+AGG 0.775132 contig478end:+22966 MsG0080048842.01.T01:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTATTTATTTTTTAAT+TGG + contig478end:20306-20325 MsG0080048842.01.T01:intron 0.0%
!! AAAATGAAAGAAAAAAAAAA+GGG - contig478end:21356-21375 None:intergenic 10.0%
!! AATATGGTAAAAAAAAAAAT+CGG - contig478end:21316-21335 None:intergenic 10.0%
!!! ATTTTTTTTTTAAAAGCAAT+TGG + contig478end:20361-20380 MsG0080048842.01.T01:intron 10.0%
!!! TAAATATAAGATTAGTTAAT+TGG - contig478end:21226-21245 None:intergenic 10.0%
!!! TTTTTTTTTTTACCATATTT+TGG + contig478end:21317-21336 MsG0080048842.01.T01:intron 10.0%
!! AAGTAAAATGACAAAATTTA+AGG - contig478end:20340-20359 MsG0080048843.01.T01:intron 15.0%
!! ATTAGATGGTAATATATAAA+AGG + contig478end:22605-22624 MsG0080048842.01.T01:intron 15.0%
!! GAAAATGAAAGAAAAAAAAA+AGG - contig478end:21357-21376 None:intergenic 15.0%
!! GTTACTCTTAAATAAATAAA+AGG - contig478end:21136-21155 MsG0080048843.01.T01:exon 15.0%
!! TATATATTTGCTTACTAAAT+TGG + contig478end:21922-21941 MsG0080048842.01.T01:intron 15.0%
!! TTAGATGGTAATATATAAAA+GGG + contig478end:22606-22625 MsG0080048842.01.T01:intron 15.0%
!!! TATTATTTATAAGCTTGTTA+AGG - contig478end:22091-22110 None:intergenic 15.0%
!! AACTACCCAAAATAATTAAA+AGG - contig478end:21847-21866 None:intergenic 20.0%
!! CTTAAATAAATAAAAGGCAA+GGG - contig478end:21130-21149 MsG0080048843.01.T01:exon 20.0%
!! GATTATTCATAATTCAAAGT+TGG + contig478end:20393-20412 MsG0080048842.01.T01:intron 20.0%
!! GATTTATACTCAATTTAAAG+AGG + contig478end:21284-21303 MsG0080048842.01.T01:intron 20.0%
!! TAAATAAATAACTGATAGAG+AGG - contig478end:20293-20312 MsG0080048843.01.T01:intron 20.0%
!! TAGATGGTAATATATAAAAG+GGG + contig478end:22607-22626 MsG0080048842.01.T01:intron 20.0%
!! TCTTAAATAAATAAAAGGCA+AGG - contig478end:21131-21150 MsG0080048843.01.T01:exon 20.0%
!! TGAAGAATGATATAATGAAA+AGG - contig478end:20049-20068 MsG0080048843.01.T01:intron 20.0%
!! TTACATGTATATCTAATTGT+TGG + contig478end:20512-20531 MsG0080048842.01.T01:intron 20.0%
!!! ATTCCTCCTTTTAATTATTT+TGG + contig478end:21838-21857 MsG0080048842.01.T01:intron 20.0%
!!! TATAAAAACATGTTGATTGT+CGG - contig478end:20941-20960 MsG0080048843.01.T01:exon 20.0%
!!! TATTTGTTGTATCAACTAAA+GGG + contig478end:23093-23112 MsG0080048842.01.T01:exon 20.0%
!!! TTCCTCCTTTTAATTATTTT+GGG + contig478end:21839-21858 MsG0080048842.01.T01:intron 20.0%
! AAAAGCTACATAAAAGACTT+AGG - contig478end:22181-22200 None:intergenic 25.0%
! AAAGAAATGCTCATTGAAAA+GGG - contig478end:22545-22564 None:intergenic 25.0%
! AAAGCTACATAAAAGACTTA+GGG - contig478end:22180-22199 None:intergenic 25.0%
! ACGGTTAATCAACAAATTAA+TGG + contig478end:20481-20500 MsG0080048842.01.T01:intron 25.0%
! AGATGGTAATATATAAAAGG+GGG + contig478end:22608-22627 MsG0080048842.01.T01:intron 25.0%
! ATATTTGAGAAATTGTGCTA+AGG + contig478end:21950-21969 MsG0080048842.01.T01:intron 25.0%
! GTAAAAAAAAAAATCGGATC+TGG - contig478end:21310-21329 None:intergenic 25.0%
! TACCCAAAATAATTAAAAGG+AGG - contig478end:21844-21863 None:intergenic 25.0%
! TATTTGAGAAATTGTGCTAA+GGG + contig478end:21951-21970 MsG0080048842.01.T01:intron 25.0%
! TGTTAAACTAGACAACAATT+TGG + contig478end:22357-22376 MsG0080048842.01.T01:intron 25.0%
!! CATTTTAGATCAGTATAGTT+CGG + contig478end:21769-21788 MsG0080048842.01.T01:intron 25.0%
!! TAAGTCTTTTATGTAGCTTT+TGG + contig478end:22180-22199 MsG0080048842.01.T01:intron 25.0%
!! TTAGTTGATACAACAAATAC+TGG - contig478end:23092-23111 None:intergenic 25.0%
!!! GATTTCTAGTTTCTATTAGA+TGG + contig478end:22591-22610 MsG0080048842.01.T01:intron 25.0%
!!! GTATTTGTTGTATCAACTAA+AGG + contig478end:23092-23111 MsG0080048842.01.T01:exon 25.0%
!!! TTTTTCAGTTGAGAAGTTTA+TGG + contig478end:21892-21911 MsG0080048842.01.T01:intron 25.0%
AAAACAAGTTATCACCTATC+AGG - contig478end:22760-22779 None:intergenic 30.0%
AAGAAATGCTCATTGAAAAG+GGG - contig478end:22544-22563 None:intergenic 30.0%
AAGAGACATTGGAATAATCA+AGG - contig478end:20230-20249 MsG0080048843.01.T01:intron 30.0%
AAGGAAGAAGATCATAATCA+TGG + contig478end:20563-20582 MsG0080048842.01.T01:intron 30.0%
AATACAATTCCTAATGTGCT+TGG + contig478end:22211-22230 MsG0080048842.01.T01:intron 30.0%
AGAGACATTGGAATAATCAA+GGG - contig478end:20229-20248 MsG0080048843.01.T01:intron 30.0%
ATAGTTATTCTCACCTTCAA+TGG - contig478end:20270-20289 MsG0080048843.01.T01:intron 30.0%
CTAAAATAAGCCAATCCAAA+CGG + contig478end:22153-22172 MsG0080048842.01.T01:intron 30.0%
GAAAGAAATGCTCATTGAAA+AGG - contig478end:22546-22565 None:intergenic 30.0%
GTGACATATTCAACTAATCA+TGG - contig478end:22296-22315 None:intergenic 30.0%
TAGTTATTCTCACCTTCAAT+GGG - contig478end:20269-20288 MsG0080048843.01.T01:intron 30.0%
TCAGCATTGTAATCAATCTT+TGG - contig478end:21617-21636 None:intergenic 30.0%
TTATTATGAGGAACATGTGA+AGG + contig478end:21427-21446 MsG0080048842.01.T01:CDS 30.0%
! CAATTCTTGCAACAGAATTT+TGG + contig478end:23245-23264 MsG0080048842.01.T01:exon 30.0%
! TGGCATCATCAAGATATTTT+GGG - contig478end:21468-21487 None:intergenic 30.0%
! TTGGCATCATCAAGATATTT+TGG - contig478end:21469-21488 None:intergenic 30.0%
!! AACAATTTGGTGATTTTTGG+TGG + contig478end:22370-22389 MsG0080048842.01.T01:CDS 30.0%
!! ATCATCAAGATATTTTGGGA+AGG - contig478end:21464-21483 None:intergenic 30.0%
!! GACAACAATTTGGTGATTTT+TGG + contig478end:22367-22386 MsG0080048842.01.T01:intron 30.0%
!! GCAATGATTGTCTTTTGTTT+GGG + contig478end:22245-22264 MsG0080048842.01.T01:intron 30.0%
!!! CTATGTATGTCGTTTTTAAG+AGG + contig478end:21061-21080 MsG0080048842.01.T01:intron 30.0%
!!! TTATTTTGGGTAGTTTCTCT+TGG + contig478end:21852-21871 MsG0080048842.01.T01:intron 30.0%
!!! TTTTTACCATATTTTGGCTC+AGG + contig478end:21323-21342 MsG0080048842.01.T01:intron 30.0%
AAAACGACATACATAGACCT+AGG - contig478end:21057-21076 MsG0080048843.01.T01:exon 35.0%
AATCCCTATCTAACTCCTAA+AGG + contig478end:21179-21198 MsG0080048842.01.T01:intron 35.0%
AGCTTTACACAAACCTCAAA+TGG - contig478end:20423-20442 MsG0080048843.01.T01:intron 35.0%
AGTTATTCTCACCTTCAATG+GGG - contig478end:20268-20287 MsG0080048843.01.T01:intron 35.0%
ATAAACAGAGTGAGAGAGAA+AGG - contig478end:20172-20191 MsG0080048843.01.T01:intron 35.0%
ATAACAATGTTGTGTCTCCT+AGG + contig478end:21037-21056 MsG0080048842.01.T01:intron 35.0%
ATCATAACAACTGAGAATCG+TGG + contig478end:20725-20744 MsG0080048842.01.T01:intron 35.0%
ATTATGCCTGAGCCAAAATA+TGG - contig478end:21332-21351 None:intergenic 35.0%
CTTGTTGACATCTAAATGCT+CGG + contig478end:22672-22691 MsG0080048842.01.T01:intron 35.0%
GAACGTGAAAAACGAGAAAT+CGG + contig478end:20658-20677 MsG0080048842.01.T01:intron 35.0%
GATTAGGCCAACAATATTTC+TGG + contig478end:20760-20779 MsG0080048842.01.T01:intron 35.0%
GATTTCATCAATTCACCCAA+TGG + contig478end:21659-21678 MsG0080048842.01.T01:CDS 35.0%
GCTTTACACAAACCTCAAAT+GGG - contig478end:20422-20441 MsG0080048843.01.T01:intron 35.0%
GTCTTTGTGACTAACATTGT+TGG + contig478end:22334-22353 MsG0080048842.01.T01:intron 35.0%
GTGTAAAGGTTAGAACTTGA+AGG - contig478end:20135-20154 MsG0080048843.01.T01:intron 35.0%
TAAACAGAGTGAGAGAGAAA+GGG - contig478end:20171-20190 MsG0080048843.01.T01:intron 35.0%
TATGTCACAAACAACCTGAT+AGG + contig478end:22743-22762 MsG0080048842.01.T01:intron 35.0%
TCAATGCTACAGTGATCAAT+TGG - contig478end:22573-22592 None:intergenic 35.0%
TGAAATTCTGCATCCTCTAA+TGG + contig478end:20080-20099 MsG0080048842.01.T01:exon 35.0%
TGACGTCTTGGAAAAATTTC+TGG + contig478end:21634-21653 MsG0080048842.01.T01:CDS 35.0%
TGCCATATAGAAAGAGTTAG+CGG + contig478end:21092-21111 MsG0080048842.01.T01:intron 35.0%
TTCTTAAACCCTTGAAGATC+CGG + contig478end:20193-20212 MsG0080048842.01.T01:CDS 35.0%
! AACTCCTTTAGGAGTTAGAT+AGG - contig478end:21186-21205 MsG0080048843.01.T01:exon 35.0%
! AAGTCTGAAGTGAGTAATGA+CGG + contig478end:20462-20481 MsG0080048842.01.T01:intron 35.0%
! ACTCCTTTAGGAGTTAGATA+GGG - contig478end:21185-21204 MsG0080048843.01.T01:exon 35.0%
! ATCTTCTTCCTTTTGATCCA+TGG - contig478end:20555-20574 MsG0080048843.01.T01:intron 35.0%
! GGCAATGATTGTCTTTTGTT+TGG + contig478end:22244-22263 MsG0080048842.01.T01:intron 35.0%
! TATGCATTGACAAAACGACA+AGG + contig478end:22966-22985 MsG0080048842.01.T01:exon 35.0%
! TTCCTTTTGATCCATGGATT+CGG - contig478end:20549-20568 MsG0080048843.01.T01:intron 35.0%
!! CCAACAATATTTCTGGTGTA+CGG + contig478end:20767-20786 MsG0080048842.01.T01:intron 35.0%
!! GTTTGCGAAATTTGGTGTAA+AGG - contig478end:20149-20168 MsG0080048843.01.T01:intron 35.0%
!! TGTGTAAAGCTTGTTGAATC+TGG + contig478end:20432-20451 MsG0080048842.01.T01:intron 35.0%
!!! AATTTGGTGATTTTTGGTGG+AGG + contig478end:22373-22392 MsG0080048842.01.T01:CDS 35.0%
!!! ATTTGGTGATTTTTGGTGGA+GGG + contig478end:22374-22393 MsG0080048842.01.T01:CDS 35.0%
AACAGTGCTCACATCAGAAA+TGG - contig478end:21492-21511 None:intergenic 40.0%
AATGGTCCAGTAAAACAACC+AGG + contig478end:21677-21696 MsG0080048842.01.T01:CDS 40.0%
AATTCCTAATGTGCTTGGCA+GGG + contig478end:22216-22235 MsG0080048842.01.T01:intron 40.0%
AATTGTGCTAAGGGTCTGTT+TGG + contig478end:21960-21979 MsG0080048842.01.T01:intron 40.0%
ACCATACCTGTCAAGTTCAT+AGG - contig478end:22799-22818 None:intergenic 40.0%
ACCGCACTACTATTGTTGAT+TGG + contig478end:21567-21586 MsG0080048842.01.T01:CDS 40.0%
AGGAAGGAAACATGCATGTT+TGG + contig478end:20796-20815 MsG0080048842.01.T01:intron 40.0%
AGGTTAGAACTTGAAGGTGA+AGG - contig478end:20129-20148 MsG0080048843.01.T01:intron 40.0%
CAACTAAAGGGTCTCCTAAA+AGG + contig478end:23105-23124 MsG0080048842.01.T01:exon 40.0%
CAACTCTTTGCAGATTTGGA+GGG + contig478end:23059-23078 MsG0080048842.01.T01:exon 40.0%
CATATAGAAAGAGTTAGCGG+CGG + contig478end:21095-21114 MsG0080048842.01.T01:intron 40.0%
CCAAATCTGCAAAGAGTTGA+AGG - contig478end:23058-23077 None:intergenic 40.0%
CCATACCTGTCAAGTTCATA+GGG - contig478end:22798-22817 None:intergenic 40.0%
CCATTTGCGTTTAACACTCT+AGG + contig478end:20961-20980 MsG0080048842.01.T01:intron 40.0%
CCGTACACCAGAAATATTGT+TGG - contig478end:20770-20789 MsG0080048843.01.T01:intron 40.0%
CCTTCAACTCTTTGCAGATT+TGG + contig478end:23055-23074 MsG0080048842.01.T01:exon 40.0%
GAAGACATTGCTCAACGTAA+TGG + contig478end:21533-21552 MsG0080048842.01.T01:CDS 40.0%
GAGAAAGGGTTTGCGAAATT+TGG - contig478end:20157-20176 MsG0080048843.01.T01:intron 40.0%
GAGTTAGTCGTAACTCCTTT+AGG - contig478end:21197-21216 None:intergenic 40.0%
GCATCAAATTGATGAACGCA+CGG + contig478end:20700-20719 MsG0080048842.01.T01:intron 40.0%
GCCAATCAACAATAGTAGTG+CGG - contig478end:21571-21590 None:intergenic 40.0%
GTCGCTTAAAAGTTGACACA+TGG + contig478end:23272-23291 MsG0080048842.01.T01:exon 40.0%
TAAAAGACTTAGGGTCCGTT+TGG - contig478end:22171-22190 None:intergenic 40.0%
TAAAGGAGTGAGCAAGTTTG+TGG + contig478end:23160-23179 MsG0080048842.01.T01:exon 40.0%
TCAACTCTTTGCAGATTTGG+AGG + contig478end:23058-23077 MsG0080048842.01.T01:exon 40.0%
TCTTAAACCCTTGAAGATCC+GGG + contig478end:20194-20213 MsG0080048842.01.T01:CDS 40.0%
TGACAAATCTTCCCCATTGA+AGG + contig478end:20254-20273 MsG0080048842.01.T01:CDS 40.0%
TGATTACAATGCTGACGTCT+TGG + contig478end:21622-21641 MsG0080048842.01.T01:CDS 40.0%
TGCATATCTGCTTTCGACAA+TGG - contig478end:22952-22971 None:intergenic 40.0%
TTCCGAATCCATGGATCAAA+AGG + contig478end:20544-20563 MsG0080048842.01.T01:intron 40.0%
TTGTTTCCATGCTGGAATCT+CGG - contig478end:22507-22526 None:intergenic 40.0%
TTTGTCACTGGAAGAGACAT+TGG - contig478end:20241-20260 MsG0080048843.01.T01:intron 40.0%
! ACTACTATTGTTGATTGGCC+TGG + contig478end:21572-21591 MsG0080048842.01.T01:CDS 40.0%
! ATAGTACCTTGTTTCCATGC+TGG - contig478end:22515-22534 None:intergenic 40.0%
! ATCTCAGCCTTAGACCTTTT+AGG - contig478end:23122-23141 None:intergenic 40.0%
! ATTGCTTGAAGCAGCTTTGA+GGG - contig478end:20885-20904 MsG0080048843.01.T01:exon 40.0%
! CATGTGATTTGTACTGCGAT+TGG - contig478end:22899-22918 None:intergenic 40.0%
! CCTAGAGTGTTAAACGCAAA+TGG - contig478end:20964-20983 MsG0080048843.01.T01:exon 40.0%
! GTGGTCCTTTGAGAATGATT+AGG + contig478end:20744-20763 MsG0080048842.01.T01:intron 40.0%
! GTGTCAACTTTTAAGCGACA+CGG - contig478end:23271-23290 None:intergenic 40.0%
! GTTGGCCTAATCATTCTCAA+AGG - contig478end:20752-20771 MsG0080048843.01.T01:intron 40.0%
! TAAAAGGTCTAAGGCTGAGA+TGG + contig478end:23121-23140 MsG0080048842.01.T01:exon 40.0%
! TTTTCCATCAGACTAGCAAC+TGG + contig478end:21372-21391 MsG0080048842.01.T01:intron 40.0%
!! GGTTGTTTTACTGGACCATT+GGG - contig478end:21677-21696 None:intergenic 40.0%
!! TCAGTACCTGGTTGTTTTAC+TGG - contig478end:21686-21705 None:intergenic 40.0%
!! TCTTCTAAGAGGCACCTTAA+AGG + contig478end:22818-22837 MsG0080048842.01.T01:exon 40.0%
!! TGGTTGTTTTACTGGACCAT+TGG - contig478end:21678-21697 None:intergenic 40.0%
AAAACCCTGCCAAGCACATT+AGG - contig478end:22223-22242 None:intergenic 45.0%
AAAGCAGGATACTCCTCTTG+TGG - contig478end:23206-23225 None:intergenic 45.0%
AATCAAGGGTAGTTGAAGCC+CGG - contig478end:20215-20234 MsG0080048843.01.T01:intron 45.0%
ACCTGTCAAGTTCATAGGGA+AGG - contig478end:22794-22813 None:intergenic 45.0%
AGAGTTGAAGGAACTCCTCA+CGG - contig478end:23046-23065 None:intergenic 45.0%
AGGGTCTCCTAAAAGGTCTA+AGG + contig478end:23112-23131 MsG0080048842.01.T01:exon 45.0%
CAAAGCTGCTTCAAGCAATG+TGG + contig478end:20886-20905 MsG0080048842.01.T01:intron 45.0%
CAAAGTTGGATGCCCATTTG+AGG + contig478end:20407-20426 MsG0080048842.01.T01:intron 45.0%
CAACTGGCAGTGGAAATTCA+AGG + contig478end:21388-21407 MsG0080048842.01.T01:CDS 45.0%
CAATGGGGAAGATTTGTCAC+TGG - contig478end:20253-20272 MsG0080048843.01.T01:intron 45.0%
CAATTCCTAATGTGCTTGGC+AGG + contig478end:22215-22234 MsG0080048842.01.T01:intron 45.0%
CATTGCTTGAAGCAGCTTTG+AGG - contig478end:20886-20905 MsG0080048843.01.T01:exon 45.0%
CCCTATGAACTTGACAGGTA+TGG + contig478end:22795-22814 MsG0080048842.01.T01:intron 45.0%
CCTGTCAAGTTCATAGGGAA+GGG - contig478end:22793-22812 None:intergenic 45.0%
CGATCAGAATAACCTAGACC+AGG - contig478end:21593-21612 None:intergenic 45.0%
CGCCGCTAACTCTTTCTATA+TGG - contig478end:21097-21116 MsG0080048843.01.T01:exon 45.0%
GAAGCTCTTAACGGAGCTAA+AGG + contig478end:23143-23162 MsG0080048842.01.T01:exon 45.0%
GAATCTCGGCCAAATACGAT+AGG - contig478end:22493-22512 None:intergenic 45.0%
GACAGGTATGGTCTTCTAAG+AGG + contig478end:22807-22826 MsG0080048842.01.T01:intron 45.0%
GAGATTCCAGCATGGAAACA+AGG + contig478end:22506-22525 MsG0080048842.01.T01:CDS 45.0%
GCAACAGCAGCTATAGCTTT+GGG - contig478end:22448-22467 None:intergenic 45.0%
GCTACAGTGATCAATTGGCA+AGG - contig478end:22568-22587 None:intergenic 45.0%
GCTGAGATGGAAGCTCTTAA+CGG + contig478end:23134-23153 MsG0080048842.01.T01:exon 45.0%
GGAACATGTGAAGGAGAGTT+CGG + contig478end:21436-21455 MsG0080048842.01.T01:CDS 45.0%
GTGCTCACATCAGAAATGGT+TGG - contig478end:21488-21507 None:intergenic 45.0%
TATCTGCTTTCGACAATGGC+TGG - contig478end:22948-22967 None:intergenic 45.0%
TCGGCCAAATACGATAGGAA+GGG - contig478end:22488-22507 None:intergenic 45.0%
TCTAGGTTTCCAAGCACTAG+AGG + contig478end:20978-20997 MsG0080048842.01.T01:intron 45.0%
TGCAACAGCAGCTATAGCTT+TGG - contig478end:22449-22468 None:intergenic 45.0%
TGCATGTTTGGACTTGACTG+AGG + contig478end:20808-20827 MsG0080048842.01.T01:intron 45.0%
TGTGTTGTGTTCCGAATCCA+TGG + contig478end:20535-20554 MsG0080048842.01.T01:intron 45.0%
TTGATGCACGAGCATCAACA+TGG - contig478end:20687-20706 MsG0080048843.01.T01:intron 45.0%
! AATGTGCTTGGCAGGGTTTT+AGG + contig478end:22223-22242 MsG0080048842.01.T01:intron 45.0%
! TAGAGAGCATGTTGATGCTC+AGG + contig478end:20592-20611 MsG0080048842.01.T01:intron 45.0%
! TTCTGATGTGAGCACTGTTG+AGG + contig478end:21493-21512 MsG0080048842.01.T01:CDS 45.0%
! TTTCTGGTGTACGGATGAGT+AGG + contig478end:20776-20795 MsG0080048842.01.T01:intron 45.0%
!! ATTGTTGATTGGCCTGGTCT+AGG + contig478end:21578-21597 MsG0080048842.01.T01:CDS 45.0%
!! CAACCAGGTACTGATAGTAG+TGG + contig478end:21692-21711 MsG0080048842.01.T01:intron 45.0%
!! TGGTGTCACGTTATCAGGAT+TGG - contig478end:22928-22947 None:intergenic 45.0%
!! TTTAATAAATATTTATTATG+AGG + contig478end:21415-21434 MsG0080048842.01.T01:CDS 5.0%
AACTCCTCACGGGATGTTAC+AGG - contig478end:23035-23054 None:intergenic 50.0%
ACTCCTCACGGGATGTTACA+GGG - contig478end:23034-23053 None:intergenic 50.0%
ACTGAGGTTTCCCCACTTGT+GGG + contig478end:20824-20843 MsG0080048842.01.T01:intron 50.0%
ACTTGTGGGATTGAGAGACG+CGG + contig478end:20838-20857 MsG0080048842.01.T01:intron 50.0%
AGAGACGCGGCTTTCACTTT+GGG + contig478end:20851-20870 MsG0080048842.01.T01:intron 50.0%
AGTAGTCCGTGCTGCGAAAA+AGG + contig478end:22411-22430 MsG0080048842.01.T01:CDS 50.0%
AGTTGAAGCCCGGATCTTCA+AGG - contig478end:20205-20224 MsG0080048843.01.T01:intron 50.0%
ATCAGACTAGCAACTGGCAG+TGG + contig478end:21378-21397 MsG0080048842.01.T01:intron 50.0%
ATTTGGCCGAGATTCCAGCA+TGG + contig478end:22498-22517 MsG0080048842.01.T01:CDS 50.0%
CCCTTCCCTATGAACTTGAC+AGG + contig478end:22790-22809 MsG0080048842.01.T01:intron 50.0%
CCTCCACTACTATCAGTACC+TGG - contig478end:21698-21717 None:intergenic 50.0%
CGGCCAAATACGATAGGAAG+GGG - contig478end:22487-22506 None:intergenic 50.0%
CGTCTCTCAATCCCACAAGT+GGG - contig478end:20838-20857 MsG0080048843.01.T01:intron 50.0%
CTCGGCCAAATACGATAGGA+AGG - contig478end:22489-22508 None:intergenic 50.0%
GACTTAGGGTCCGTTTGGAT+TGG - contig478end:22166-22185 None:intergenic 50.0%
GAGTTGAAGGAACTCCTCAC+GGG - contig478end:23045-23064 None:intergenic 50.0%
GATTCACAGCCTCTAGTGCT+TGG - contig478end:20990-21009 MsG0080048843.01.T01:exon 50.0%
GGTCCCCTTCCTATCGTATT+TGG + contig478end:22481-22500 MsG0080048842.01.T01:CDS 50.0%
GTAGTCCGTGCTGCGAAAAA+GGG + contig478end:22412-22431 MsG0080048842.01.T01:CDS 50.0%
GTCTCTCAATCCCACAAGTG+GGG - contig478end:20837-20856 MsG0080048843.01.T01:intron 50.0%
GTTGAAGCCCGGATCTTCAA+GGG - contig478end:20204-20223 MsG0080048843.01.T01:intron 50.0%
TGGTGCTCTTCTTCCACAAG+AGG + contig478end:23190-23209 MsG0080048842.01.T01:exon 50.0%
TGGTGTACGGATGAGTAGGA+AGG + contig478end:20780-20799 MsG0080048842.01.T01:intron 50.0%
! ACTGCCAGTTGCTAGTCTGA+TGG - contig478end:21379-21398 None:intergenic 50.0%
! ATGTGAGCACTGTTGAGGAG+TGG + contig478end:21498-21517 MsG0080048842.01.T01:CDS 50.0%
! GGAGTATCCTGCTTTAGTCG+CGG + contig478end:23211-23230 MsG0080048842.01.T01:exon 50.0%
!! AGCAAGTTTGTGGAAGTGCC+TGG + contig478end:23170-23189 MsG0080048842.01.T01:exon 50.0%
!! ATGGCTGGTGTCACGTTATC+AGG - contig478end:22933-22952 None:intergenic 50.0%
!! CCAGGTACTGATAGTAGTGG+AGG + contig478end:21695-21714 MsG0080048842.01.T01:intron 50.0%
!! GTTGCAGAGAGTGCCATTAG+AGG - contig478end:20096-20115 MsG0080048843.01.T01:intron 50.0%
!! TCTTGTGGAAGAAGAGCACC+AGG - contig478end:23191-23210 None:intergenic 50.0%
AGACCCTGTAACATCCCGTG+AGG + contig478end:23028-23047 MsG0080048842.01.T01:exon 55.0%
AGCTCTTCCGCGACTAAAGC+AGG - contig478end:23221-23240 None:intergenic 55.0%
AGTCCAGTCAAGAAAGCCGC+GGG - contig478end:23005-23024 None:intergenic 55.0%
CACGGGATGTTACAGGGTCT+AGG - contig478end:23028-23047 None:intergenic 55.0%
GACTGAGGTTTCCCCACTTG+TGG + contig478end:20823-20842 MsG0080048842.01.T01:intron 55.0%
GAGAGACGCGGCTTTCACTT+TGG + contig478end:20850-20869 MsG0080048842.01.T01:intron 55.0%
GAGTCCAGTCAAGAAAGCCG+CGG - contig478end:23006-23025 None:intergenic 55.0%
GCGTCTCTCAATCCCACAAG+TGG - contig478end:20839-20858 MsG0080048843.01.T01:intron 55.0%
TACACCATCCACCCAACTGC+AGG - contig478end:22846-22865 None:intergenic 55.0%
TAGCTGCTGTTGCACCCACT+TGG + contig478end:22455-22474 MsG0080048842.01.T01:CDS 55.0%
TTACCCGCGGCTTTCTTGAC+TGG + contig478end:22999-23018 MsG0080048842.01.T01:exon 55.0%
! AGGAGCACGTTACTTACCCG+CGG + contig478end:22986-23005 MsG0080048842.01.T01:exon 55.0%
!! ACCTTAAAGGCGCCTGCAGT+TGG + contig478end:22831-22850 MsG0080048842.01.T01:exon 55.0%
!! CCAGACCCTTTTTCGCAGCA+CGG - contig478end:22420-22439 None:intergenic 55.0%
!! CCTTAAAGGCGCCTGCAGTT+GGG + contig478end:22832-22851 MsG0080048842.01.T01:exon 55.0%
AGGAAGGGGACCAGACCAAG+TGG - contig478end:22473-22492 None:intergenic 60.0%
CCCAACTGCAGGCGCCTTTA+AGG - contig478end:22835-22854 None:intergenic 60.0%
CCGTGCTGCGAAAAAGGGTC+TGG + contig478end:22417-22436 MsG0080048842.01.T01:CDS 60.0%
GCTGTTGCACCCACTTGGTC+TGG + contig478end:22460-22479 MsG0080048842.01.T01:CDS 60.0%
GGAAGGGGACCAGACCAAGT+GGG - contig478end:22472-22491 None:intergenic 60.0%
!! TAAAGGCGCCTGCAGTTGGG+TGG + contig478end:22835-22854 MsG0080048842.01.T01:exon 60.0%
GGCGCCTGCAGTTGGGTGGA+TGG + contig478end:22839-22858 MsG0080048842.01.T01:exon 70.0%
Chromosome Type Strat End Strand Name
contig478end gene 20043 23296 20043 ID=MsG0080048842.01;
contig478end mRNA 20043 23296 20043 ID=MsG0080048842.01.T01;Parent=MsG0080048842.01
contig478end exon 20043 20275 20043 ID=MsG0080048842.01.T01.exon;Parent=MsG0080048842.01.T01
contig478end CDS 20099 20275 20099 ID=MsG0080048842.01.T01.cds;Parent=MsG0080048842.01.T01
contig478end CDS 21383 21698 21383 ID=MsG0080048842.01.T01.cds;Parent=MsG0080048842.01.T01
contig478end exon 21383 21698 21383 ID=MsG0080048842.01.T01.exon;Parent=MsG0080048842.01.T01
contig478end CDS 22368 22527 22368 ID=MsG0080048842.01.T01.cds;Parent=MsG0080048842.01.T01
contig478end exon 22368 22527 22368 ID=MsG0080048842.01.T01.exon;Parent=MsG0080048842.01.T01
contig478end CDS 22812 23280 22812 ID=MsG0080048842.01.T01.cds;Parent=MsG0080048842.01.T01
contig478end exon 22812 23296 22812 ID=MsG0080048842.01.T01.exon;Parent=MsG0080048842.01.T01
Gene Sequence

>MsG0080048842.01.T01

ATGGCACTCTCTGCAACATTTATCACTCCTTCACCTTCAAGTTCTAACCTTTACACCAAATTTCGCAAACCCTTTCTCTCTCACTCTGTTTATCTTCTTAAACCCTTGAAGATCCGGGCTTCAACTACCCTTGATTATTCCAATGTCTCTTCCAGTGACAAATCTTCCCCATTGAAGACTAGCAACTGGCAGTGGAAATTCAAGGACAATTTAATAAATATTTATTATGAGGAACATGTGAAGGAGAGTTCGGAGCCTTCCCAAAATATCTTGATGATGCCAACCATTTCTGATGTGAGCACTGTTGAGGAGTGGAGATTAGTTGCTGAAGACATTGCTCAACGTAATGGCAGTGTCAATTACCGCACTACTATTGTTGATTGGCCTGGTCTAGGTTATTCTGATCGACCAAAGATTGATTACAATGCTGACGTCTTGGAAAAATTTCTGGTTGATTTCATCAATTCACCCAATGGTCCAGTAAAACAACCAGACAACAATTTGGTGATTTTTGGTGGAGGGCATGCTGCTTCAATAGTAGTCCGTGCTGCGAAAAAGGGTCTGGTGAAGCCCAAAGCTATAGCTGCTGTTGCACCCACTTGGTCTGGTCCCCTTCCTATCGTATTTGGCCGAGATTCCAGCATGGAAACAAGGTATGGTCTTCTAAGAGGCACCTTAAAGGCGCCTGCAGTTGGGTGGATGGTGTATAACATGCTTGTGAGCAATGAGAATGCAATCCAATCGCAGTACAAATCACATGTATATGCCAATCCTGATAACGTGACACCAGCCATTGTCGAAAGCAGATATGCATTGACAAAACGACAAGGAGCACGTTACTTACCCGCGGCTTTCTTGACTGGACTCCTAGACCCTGTAACATCCCGTGAGGAGTTCCTTCAACTCTTTGCAGATTTGGAGGGAAAAATACCAGTATTTGTTGTATCAACTAAAGGGTCTCCTAAAAGGTCTAAGGCTGAGATGGAAGCTCTTAACGGAGCTAAAGGAGTGAGCAAGTTTGTGGAAGTGCCTGGTGCTCTTCTTCCACAAGAGGAGTATCCTGCTTTAGTCGCGGAAGAGCTTTATCAATTCTTGCAACAGAATTTTGGCCGTGTCGCTTAA
Protein sequence

>MsG0080048842.01.T01

MALSATFITPSPSSSNLYTKFRKPFLSHSVYLLKPLKIRASTTLDYSNVSSSDKSSPLKTSNWQWKFKDNLINIYYEEHVKESSEPSQNILMMPTISDVSTVEEWRLVAEDIAQRNGSVNYRTTIVDWPGLGYSDRPKIDYNADVLEKFLVDFINSPNGPVKQPDNNLVIFGGGHAASIVVRAAKKGLVKPKAIAAVAPTWSGPLPIVFGRDSSMETRYGLLRGTLKAPAVGWMVYNMLVSNENAIQSQYKSHVYANPDNVTPAIVESRYALTKRQGARYLPAAFLTGLLDPVTSREEFLQLFADLEGKIPVFVVSTKGSPKRSKAEMEALNGAKGVSKFVEVPGALLPQEEYPALVAEELYQFLQQNFGRVA*