AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006310.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006310.01.T01 MTR_2g005740 93.168 161 3 1 1 153 1 161 9.06e-108 304
MsG0280006310.01.T01 MTR_2g005740 92.715 151 3 1 11 153 1 151 1.06e-99 283
MsG0280006310.01.T01 MTR_2g012780 45.714 105 48 2 46 147 53 151 4.94e-24 92.0
MsG0280006310.01.T01 MTR_4g090440 45.833 120 46 5 37 148 25 133 5.04e-23 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006310.01.T01 AT1G09812 47.059 136 59 3 8 143 3 125 1.03e-29 105
MsG0280006310.01.T01 AT1G58007 49.524 105 43 3 41 145 20 114 1.40e-23 89.4
MsG0280006310.01.T01 AT1G58007 49.524 105 43 3 41 145 20 114 1.40e-23 89.4
MsG0280006310.01.T01 AT1G58007 49.524 105 43 3 41 145 20 114 1.40e-23 89.4
MsG0280006310.01.T01 AT1G11120 46.341 123 58 4 31 146 24 145 1.86e-22 87.8
MsG0280006310.01.T01 AT1G11120 39.865 148 57 5 31 146 24 171 1.98e-19 80.5
MsG0280006310.01.T01 AT1G58007 81.250 32 6 0 41 72 20 51 1.69e-11 56.6
MsG0280006310.01.T01 AT4G28170 37.864 103 45 5 32 132 50 135 3.01e-11 58.2

Find 53 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAAGAGACTAAGAGATTAT+TGG 0.226828 2:-312760 MsG0280006310.01.T01:three_prime_UTR
GATTTCAATGTTTCTAATAA+TGG 0.253747 2:-313192 MsG0280006310.01.T01:CDS
TTTGAACAAATGTTCAAATA+TGG 0.259962 2:+313169 None:intergenic
GTCCAAGGGAAGAAGCTTAT+TGG 0.267181 2:-312877 MsG0280006310.01.T01:CDS
GTGTGAAGCATTTAAGAAAT+TGG 0.309557 2:-313580 MsG0280006310.01.T01:intron
TAGAAACATTGAAATCAAAA+TGG 0.315037 2:+313199 None:intergenic
AAAGAGACTAAGAGATTATT+GGG 0.315819 2:-312759 MsG0280006310.01.T01:three_prime_UTR
ATCTATGCTTCCAACTTATC+AGG 0.349676 2:-313117 MsG0280006310.01.T01:CDS
TCTATGAGTGCAATATCATT+TGG 0.374376 2:-313236 MsG0280006310.01.T01:CDS
GAAGGTCCATGGAAAAGCTT+TGG 0.389743 2:-313263 MsG0280006310.01.T01:CDS
CATCATTTACTCATTGACTT+TGG 0.395198 2:+312687 None:intergenic
GGCCAATAAGCTTCTTCCCT+TGG 0.399066 2:+312875 None:intergenic
GTCTTATGCATGTGATTTGT+CGG 0.403359 2:-312957 MsG0280006310.01.T01:CDS
ATGTAAGCCATGTTTCCATT+TGG 0.438502 2:-312717 MsG0280006310.01.T01:three_prime_UTR
TGATTTGTCGGTGTTGATGC+CGG 0.444835 2:-312945 MsG0280006310.01.T01:CDS
TGTTAAATACAAAATGTTGT+TGG 0.457080 2:+312846 None:intergenic
CTTGGACAAGGCAGAGGAGC+AGG 0.461138 2:+312893 None:intergenic
ACAAGGCAGAGGAGCAGGTT+GGG 0.462248 2:+312898 None:intergenic
GATTTGTCGGTGTTGATGCC+GGG 0.464814 2:-312944 MsG0280006310.01.T01:CDS
AATTAGAACAAAGACAAAAC+AGG 0.469285 2:-313400 MsG0280006310.01.T01:five_prime_UTR
TCTTCCCTTGGACAAGGCAG+AGG 0.486050 2:+312887 None:intergenic
AAGGTCCATGGAAAAGCTTT+GGG 0.488752 2:-313262 MsG0280006310.01.T01:CDS
TAAGTTGGAAGCATAGATTC+TGG 0.501581 2:+313122 None:intergenic
CTGCTCCTCTGCCTTGTCCA+AGG 0.512022 2:-312892 MsG0280006310.01.T01:CDS
CCTCCTGTCTGTGAATGTGA+TGG 0.513542 2:+313299 None:intergenic
GACTTTGGAGAACAGCCAAA+TGG 0.513956 2:+312702 None:intergenic
ACAAAATGTTGTTGGTGAGA+AGG 0.517710 2:+312854 None:intergenic
TTCTGCCTCTTGAGACAGCA+AGG 0.520372 2:-313774 None:intergenic
TGTGAGATCAGAATGAAGTT+TGG 0.528238 2:+313553 None:intergenic
TGCTCCTCTGCCTTGTCCAA+GGG 0.530867 2:-312891 MsG0280006310.01.T01:CDS
GACAAGGCAGAGGAGCAGGT+TGG 0.540635 2:+312897 None:intergenic
GTATACACGGCTTCAAACGC+AGG 0.541704 2:-313323 MsG0280006310.01.T01:CDS
TAAGTAGGATACTGCTGCCC+CGG 0.546135 2:+312926 None:intergenic
GAGTTGATGAGGAAGGTCCA+TGG 0.553113 2:-313274 MsG0280006310.01.T01:CDS
CATTGAAATCAAAATGGCTG+TGG 0.558224 2:+313205 None:intergenic
GAGTGGTGTTCCTGATAAGT+TGG 0.558569 2:+313107 None:intergenic
GACAGGAGGAGGAGTTGATG+AGG 0.568497 2:-313285 MsG0280006310.01.T01:CDS
GCAGGTTGGGCAATGTAAGT+AGG 0.571068 2:+312911 None:intergenic
TAAGTTTGCTTCATCTGACA+AGG 0.587342 2:-313728 MsG0280006310.01.T01:intron
GGTCCATCACATTCACAGAC+AGG 0.587985 2:-313302 MsG0280006310.01.T01:CDS
TCACATTCACAGACAGGAGG+AGG 0.591725 2:-313296 MsG0280006310.01.T01:CDS
GGAGGAGGAGTTGATGAGGA+AGG 0.605051 2:-313281 MsG0280006310.01.T01:CDS
TAAGCTTCTTCCCTTGGACA+AGG 0.606151 2:+312881 None:intergenic
TCACTACCAACCACAAAACA+AGG 0.611869 2:-313086 MsG0280006310.01.T01:CDS
AGTCAATGAGTAAATGATGA+CGG 0.613545 2:-312683 MsG0280006310.01.T01:three_prime_UTR
GTGGAAGAAAAGTGTTGTGT+GGG 0.618638 2:+313146 None:intergenic
AGAACAGCCAAATGGAAACA+TGG 0.620971 2:+312710 None:intergenic
ACAGTGAAAGATCAAAGATG+TGG 0.629582 2:-313349 MsG0280006310.01.T01:CDS
CGTGGAAGAAAAGTGTTGTG+TGG 0.639502 2:+313145 None:intergenic
ATTTGTCGGTGTTGATGCCG+GGG 0.648111 2:-312943 MsG0280006310.01.T01:CDS
AAAGATGTGGAACGTATACA+CGG 0.657288 2:-313336 MsG0280006310.01.T01:CDS
TGGAAGCATAGATTCTGGCG+TGG 0.678051 2:+313127 None:intergenic
CCATCACATTCACAGACAGG+AGG 0.694212 2:-313299 MsG0280006310.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAAATAAAAAAGAATAA+TGG + Chr2:312692-312711 None:intergenic 10.0%
!! AAGAAATAAAAAAGAATAAT+GGG + Chr2:312691-312710 None:intergenic 10.0%
!! ATATATTCTCAAAACATTTA+AGG - Chr2:312945-312964 MsG0280006310.01.T01:CDS 15.0%
!! CATTATACATATGTTAAAAA+AGG + Chr2:313428-313447 None:intergenic 15.0%
!! GTATGTTTATTTATTATTAC+AGG - Chr2:313449-313468 MsG0280006310.01.T01:intron 15.0%
!!! CATCATACTTTTTTTTTTAT+TGG - Chr2:312799-312818 MsG0280006310.01.T01:three_prime_UTR 15.0%
!! TAGAAACATTGAAATCAAAA+TGG + Chr2:313243-313262 None:intergenic 20.0%
!! TGTTAAATACAAAATGTTGT+TGG + Chr2:313596-313615 None:intergenic 20.0%
!! TTTGAACAAATGTTCAAATA+TGG + Chr2:313273-313292 None:intergenic 20.0%
!!! ATCGATTAATAACATTTTGT+AGG + Chr2:313642-313661 None:intergenic 20.0%
!!! ATTTTGTATTTAACAACTAG+TGG - Chr2:313601-313620 MsG0280006310.01.T01:intron 20.0%
!!! GATTTCAATGTTTCTAATAA+TGG - Chr2:313247-313266 MsG0280006310.01.T01:CDS 20.0%
! AAAGAGACTAAGAGATTATT+GGG - Chr2:313680-313699 MsG0280006310.01.T01:intron 25.0%
! AATTAGAACAAAGACAAAAC+AGG - Chr2:313039-313058 MsG0280006310.01.T01:intron 25.0%
! TAAAGAGACTAAGAGATTAT+TGG - Chr2:313679-313698 MsG0280006310.01.T01:intron 25.0%
AAGAATCAGACAGAATAACT+AGG + Chr2:312993-313012 None:intergenic 30.0%
AAGGAAAAAACACATGAAAC+AGG + Chr2:313409-313428 None:intergenic 30.0%
ACATGAAACAGGTAGTAAAA+GGG + Chr2:313398-313417 None:intergenic 30.0%
AGTCAATGAGTAAATGATGA+CGG - Chr2:313756-313775 MsG0280006310.01.T01:five_prime_UTR 30.0%
ATTAAGTTAAGCATCAGTTG+AGG - Chr2:312757-312776 MsG0280006310.01.T01:three_prime_UTR 30.0%
TCTATGAGTGCAATATCATT+TGG - Chr2:313203-313222 MsG0280006310.01.T01:CDS 30.0%
TTATAAAGCTTAGCTAACTG+TGG - Chr2:312822-312841 MsG0280006310.01.T01:exon 30.0%
! GTGTGAAGCATTTAAGAAAT+TGG - Chr2:312859-312878 MsG0280006310.01.T01:CDS 30.0%
!! CATCATTTACTCATTGACTT+TGG + Chr2:313755-313774 None:intergenic 30.0%
AAAGATGTGGAACGTATACA+CGG - Chr2:313103-313122 MsG0280006310.01.T01:CDS 35.0%
ACAGTGAAAGATCAAAGATG+TGG - Chr2:313090-313109 MsG0280006310.01.T01:CDS 35.0%
ATCTATGCTTCCAACTTATC+AGG - Chr2:313322-313341 MsG0280006310.01.T01:CDS 35.0%
ATGTAAGCCATGTTTCCATT+TGG - Chr2:313722-313741 MsG0280006310.01.T01:intron 35.0%
CACATGAAACAGGTAGTAAA+AGG + Chr2:313399-313418 None:intergenic 35.0%
CATTGAAATCAAAATGGCTG+TGG + Chr2:313237-313256 None:intergenic 35.0%
TGTGAGATCAGAATGAAGTT+TGG + Chr2:312889-312908 None:intergenic 35.0%
! ACAAAATGTTGTTGGTGAGA+AGG + Chr2:313588-313607 None:intergenic 35.0%
! GTCTTATGCATGTGATTTGT+CGG - Chr2:313482-313501 MsG0280006310.01.T01:intron 35.0%
! TAAGTTGGAAGCATAGATTC+TGG + Chr2:313320-313339 None:intergenic 35.0%
! TAAGTTTGCTTCATCTGACA+AGG - Chr2:312711-312730 MsG0280006310.01.T01:three_prime_UTR 35.0%
!!! TTGTGCATTTCCTTGTTTTG+TGG + Chr2:313366-313385 None:intergenic 35.0%
AAGGTCCATGGAAAAGCTTT+GGG - Chr2:313177-313196 MsG0280006310.01.T01:CDS 40.0%
AGAACAGCCAAATGGAAACA+TGG + Chr2:313732-313751 None:intergenic 40.0%
TCACTACCAACCACAAAACA+AGG - Chr2:313353-313372 MsG0280006310.01.T01:CDS 40.0%
! CATTTAAGGCTCGTCTAACT+TGG - Chr2:312959-312978 MsG0280006310.01.T01:CDS 40.0%
!! GTGGAAGAAAAGTGTTGTGT+GGG + Chr2:313296-313315 None:intergenic 40.0%
!!! GCATTTCCTTGTTTTGTGGT+TGG + Chr2:313362-313381 None:intergenic 40.0%
GAAGGTCCATGGAAAAGCTT+TGG - Chr2:313176-313195 MsG0280006310.01.T01:CDS 45.0%
GAGTGGTGTTCCTGATAAGT+TGG + Chr2:313335-313354 None:intergenic 45.0%
GTCCAAGGGAAGAAGCTTAT+TGG - Chr2:313562-313581 MsG0280006310.01.T01:five_prime_UTR 45.0%
TAAGCTTCTTCCCTTGGACA+AGG + Chr2:313561-313580 None:intergenic 45.0%
! GAAGTCCCAAAGCTTTTCCA+TGG + Chr2:313185-313204 None:intergenic 45.0%
! GACTTTGGAGAACAGCCAAA+TGG + Chr2:313740-313759 None:intergenic 45.0%
!! CGTGGAAGAAAAGTGTTGTG+TGG + Chr2:313297-313316 None:intergenic 45.0%
!! TGATTTGTCGGTGTTGATGC+CGG - Chr2:313494-313513 MsG0280006310.01.T01:intron 45.0%
!!! GTTTTGTGGTTGGTAGTGAG+TGG + Chr2:313352-313371 None:intergenic 45.0%
CCATCACATTCACAGACAGG+AGG - Chr2:313140-313159 MsG0280006310.01.T01:CDS 50.0%
CCTCCTGTCTGTGAATGTGA+TGG + Chr2:313143-313162 None:intergenic 50.0%
GGCCAATAAGCTTCTTCCCT+TGG + Chr2:313567-313586 None:intergenic 50.0%
GGTCCATCACATTCACAGAC+AGG - Chr2:313137-313156 MsG0280006310.01.T01:CDS 50.0%
GTATACACGGCTTCAAACGC+AGG - Chr2:313116-313135 MsG0280006310.01.T01:CDS 50.0%
TAAGTAGGATACTGCTGCCC+CGG + Chr2:313516-313535 None:intergenic 50.0%
TCACATTCACAGACAGGAGG+AGG - Chr2:313143-313162 MsG0280006310.01.T01:CDS 50.0%
! GCAGGTTGGGCAATGTAAGT+AGG + Chr2:313531-313550 None:intergenic 50.0%
! TGGAAGCATAGATTCTGGCG+TGG + Chr2:313315-313334 None:intergenic 50.0%
!! ATTTGTCGGTGTTGATGCCG+GGG - Chr2:313496-313515 MsG0280006310.01.T01:intron 50.0%
!! GAGTTGATGAGGAAGGTCCA+TGG - Chr2:313165-313184 MsG0280006310.01.T01:CDS 50.0%
!! GATTTGTCGGTGTTGATGCC+GGG - Chr2:313495-313514 MsG0280006310.01.T01:intron 50.0%
ACAAGGCAGAGGAGCAGGTT+GGG + Chr2:313544-313563 None:intergenic 55.0%
TCTTCCCTTGGACAAGGCAG+AGG + Chr2:313555-313574 None:intergenic 55.0%
TGCTCCTCTGCCTTGTCCAA+GGG - Chr2:313548-313567 MsG0280006310.01.T01:five_prime_UTR 55.0%
!! GACAGGAGGAGGAGTTGATG+AGG - Chr2:313154-313173 MsG0280006310.01.T01:CDS 55.0%
!! GGAGGAGGAGTTGATGAGGA+AGG - Chr2:313158-313177 MsG0280006310.01.T01:CDS 55.0%
CTGCTCCTCTGCCTTGTCCA+AGG - Chr2:313547-313566 MsG0280006310.01.T01:five_prime_UTR 60.0%
CTTGGACAAGGCAGAGGAGC+AGG + Chr2:313549-313568 None:intergenic 60.0%
GACAAGGCAGAGGAGCAGGT+TGG + Chr2:313545-313564 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 312676 313785 312676 ID=MsG0280006310.01;Name=MsG0280006310.01
Chr2 mRNA 312676 313785 312676 ID=MsG0280006310.01.T01;Parent=MsG0280006310.01;Name=MsG0280006310.01.T01;_AED=0.41;_eAED=0.41;_QI=160|1|1|1|0.33|0.25|4|165|153
Chr2 exon 312676 312990 312676 ID=MsG0280006310.01.T01:exon:590;Parent=MsG0280006310.01.T01
Chr2 exon 313073 313422 313073 ID=MsG0280006310.01.T01:exon:589;Parent=MsG0280006310.01.T01
Chr2 exon 313530 313594 313530 ID=MsG0280006310.01.T01:exon:588;Parent=MsG0280006310.01.T01
Chr2 exon 313729 313785 313729 ID=MsG0280006310.01.T01:exon:587;Parent=MsG0280006310.01.T01
Chr2 five_prime_UTR 313729 313785 313729 ID=MsG0280006310.01.T01:five_prime_utr;Parent=MsG0280006310.01.T01
Chr2 five_prime_UTR 313530 313594 313530 ID=MsG0280006310.01.T01:five_prime_utr;Parent=MsG0280006310.01.T01
Chr2 five_prime_UTR 313385 313422 313385 ID=MsG0280006310.01.T01:five_prime_utr;Parent=MsG0280006310.01.T01
Chr2 CDS 313073 313384 313073 ID=MsG0280006310.01.T01:cds;Parent=MsG0280006310.01.T01
Chr2 CDS 312841 312990 312841 ID=MsG0280006310.01.T01:cds;Parent=MsG0280006310.01.T01
Chr2 three_prime_UTR 312676 312840 312676 ID=MsG0280006310.01.T01:three_prime_utr;Parent=MsG0280006310.01.T01
Gene Sequence

>MsG0280006310.01.T01

ATGAGCGATTTAAACAGTGAAAGATCAAAGATGTGGAACGTATACACGGCTTCAAACGCAGGTCCATCACATTCACAGACAGGAGGAGGAGTTGATGAGGAAGGTCCATGGAAAAGCTTTGGGACTTCTATGAGTGCAATATCATTTGGTTTTGTTGCCACAGCCATTTTGATTTCAATGTTTCTAATAATGGCCATATTTGAACATTTGTTCAAACCCACACAACACTTTTCTTCCACGCCAGAATCTATGCTTCCAACTTATCAGGAACACCACTCACTACCAACCACAAAACAAGGAAATGCACAATCAGTTCTATCATCGTCTTATGCATGTGATTTGTCGGTGTTGATGCCGGGGCAGCAGTATCCTACTTACATTGCCCAACCTGCTCCTCTGCCTTGTCCAAGGGAAGAAGCTTATTGGCCTTCTCACCAACAACATTTTGTATTTAACAACTAG

Protein sequence

>MsG0280006310.01.T01

MSDLNSERSKMWNVYTASNAGPSHSQTGGGVDEEGPWKSFGTSMSAISFGFVATAILISMFLIMAIFEHLFKPTQHFSSTPESMLPTYQEHHSLPTTKQGNAQSVLSSSYACDLSVLMPGQQYPTYIAQPAPLPCPREEAYWPSHQQHFVFNN*