AlfalfaGEDB | MsG0280006310.01

M. sativa cultivar ZhongmuNo.1 / MsG0280006310.01

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006310.01.T01	XP_024632239.1	93.168	161	3	1	1	153	1	161	7.48E-104	304

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006310.01.T01	Q1SKW7	93.168	161	3	1	1	153	1	161	3.57e-104	304

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048150.01	MsG0280006310.01	0.804897	1.732256e-49	9.039033e-47
MsG0080048701.01	MsG0280006310.01	0.845239	4.598191e-59	7.613805e-56
MsG0080048781.01	MsG0280006310.01	0.829377	5.326564e-55	5.442352e-52
MsG0180000076.01	MsG0280006310.01	0.810409	1.169381e-50	7.043208e-48
MsG0180000111.01	MsG0280006310.01	0.827533	1.484624e-54	1.437147e-51
MsG0180000360.01	MsG0280006310.01	0.814051	1.876126e-51	1.245691e-48
MsG0180000373.01	MsG0280006310.01	0.848066	7.765948e-60	1.407913e-56
MsG0180001143.01	MsG0280006310.01	0.808061	3.726707e-50	2.109756e-47
MsG0180001272.01	MsG0280006310.01	0.810826	9.503982e-51	5.788616e-48
MsG0180003427.01	MsG0280006310.01	0.808045	3.755079e-50	2.124932e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006310.01.T01	MTR_2g005740	93.168	161	3	1	1	153	1	161	9.06e-108	304
MsG0280006310.01.T01	MTR_2g005740	92.715	151	3	1	11	153	1	151	1.06e-99	283
MsG0280006310.01.T01	MTR_2g012780	45.714	105	48	2	46	147	53	151	4.94e-24	92.0
MsG0280006310.01.T01	MTR_4g090440	45.833	120	46	5	37	148	25	133	5.04e-23	89.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006310.01.T01	AT1G09812	47.059	136	59	3	8	143	3	125	1.03e-29	105
MsG0280006310.01.T01	AT1G58007	49.524	105	43	3	41	145	20	114	1.40e-23	89.4
MsG0280006310.01.T01	AT1G58007	49.524	105	43	3	41	145	20	114	1.40e-23	89.4
MsG0280006310.01.T01	AT1G58007	49.524	105	43	3	41	145	20	114	1.40e-23	89.4
MsG0280006310.01.T01	AT1G11120	46.341	123	58	4	31	146	24	145	1.86e-22	87.8
MsG0280006310.01.T01	AT1G11120	39.865	148	57	5	31	146	24	171	1.98e-19	80.5
MsG0280006310.01.T01	AT1G58007	81.250	32	6	0	41	72	20	51	1.69e-11	56.6
MsG0280006310.01.T01	AT4G28170	37.864	103	45	5	32	132	50	135	3.01e-11	58.2

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Find 53 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAGACTAAGAGATTATT+GGG	0.315819	2:-312759	MsG0280006310.01.T01:three_prime_UTR
AAAGATGTGGAACGTATACA+CGG	0.657288	2:-313336	MsG0280006310.01.T01:CDS
AAGGTCCATGGAAAAGCTTT+GGG	0.488752	2:-313262	MsG0280006310.01.T01:CDS
AATTAGAACAAAGACAAAAC+AGG	0.469285	2:-313400	MsG0280006310.01.T01:five_prime_UTR
ACAAAATGTTGTTGGTGAGA+AGG	0.517710	2:+312854	None:intergenic
ACAAGGCAGAGGAGCAGGTT+GGG	0.462248	2:+312898	None:intergenic
ACAGTGAAAGATCAAAGATG+TGG	0.629582	2:-313349	MsG0280006310.01.T01:CDS
AGAACAGCCAAATGGAAACA+TGG	0.620971	2:+312710	None:intergenic
AGTCAATGAGTAAATGATGA+CGG	0.613545	2:-312683	MsG0280006310.01.T01:three_prime_UTR
ATCTATGCTTCCAACTTATC+AGG	0.349676	2:-313117	MsG0280006310.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGAATCAGACAGAATAACT+AGG	+	Chr2:312993-313012	None:intergenic	30.0%
AAGGAAAAAACACATGAAAC+AGG	+	Chr2:313409-313428	None:intergenic	30.0%
ACATGAAACAGGTAGTAAAA+GGG	+	Chr2:313398-313417	None:intergenic	30.0%
AGTCAATGAGTAAATGATGA+CGG	-	Chr2:313756-313775	MsG0280006310.01.T01:five_prime_UTR	30.0%
ATTAAGTTAAGCATCAGTTG+AGG	-	Chr2:312757-312776	MsG0280006310.01.T01:three_prime_UTR	30.0%
TCTATGAGTGCAATATCATT+TGG	-	Chr2:313203-313222	MsG0280006310.01.T01:CDS	30.0%
TTATAAAGCTTAGCTAACTG+TGG	-	Chr2:312822-312841	MsG0280006310.01.T01:exon	30.0%
AAAGATGTGGAACGTATACA+CGG	-	Chr2:313103-313122	MsG0280006310.01.T01:CDS	35.0%
ACAGTGAAAGATCAAAGATG+TGG	-	Chr2:313090-313109	MsG0280006310.01.T01:CDS	35.0%
ATCTATGCTTCCAACTTATC+AGG	-	Chr2:313322-313341	MsG0280006310.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	312676	313785	312676	ID=MsG0280006310.01;Name=MsG0280006310.01
Chr2	mRNA	312676	313785	312676	ID=MsG0280006310.01.T01;Parent=MsG0280006310.01;Name=MsG0280006310.01.T01;_AED=0.41;_eAED=0.41;_QI=160\|1\|1\|1\|0.33\|0.25\|4\|165\|153
Chr2	exon	312676	312990	312676	ID=MsG0280006310.01.T01:exon:590;Parent=MsG0280006310.01.T01
Chr2	exon	313073	313422	313073	ID=MsG0280006310.01.T01:exon:589;Parent=MsG0280006310.01.T01
Chr2	exon	313530	313594	313530	ID=MsG0280006310.01.T01:exon:588;Parent=MsG0280006310.01.T01
Chr2	exon	313729	313785	313729	ID=MsG0280006310.01.T01:exon:587;Parent=MsG0280006310.01.T01
Chr2	five_prime_UTR	313729	313785	313729	ID=MsG0280006310.01.T01:five_prime_utr;Parent=MsG0280006310.01.T01
Chr2	five_prime_UTR	313530	313594	313530	ID=MsG0280006310.01.T01:five_prime_utr;Parent=MsG0280006310.01.T01
Chr2	five_prime_UTR	313385	313422	313385	ID=MsG0280006310.01.T01:five_prime_utr;Parent=MsG0280006310.01.T01
Chr2	CDS	313073	313384	313073	ID=MsG0280006310.01.T01:cds;Parent=MsG0280006310.01.T01
Chr2	CDS	312841	312990	312841	ID=MsG0280006310.01.T01:cds;Parent=MsG0280006310.01.T01
Chr2	three_prime_UTR	312676	312840	312676	ID=MsG0280006310.01.T01:three_prime_utr;Parent=MsG0280006310.01.T01

Gene Sequence

>MsG0280006310.01.T01

ATGAGCGATTTAAACAGTGAAAGATCAAAGATGTGGAACGTATACACGGCTTCAAACGCAGGTCCATCACATTCACAGACAGGAGGAGGAGTTGATGAGGAAGGTCCATGGAAAAGCTTTGGGACTTCTATGAGTGCAATATCATTTGGTTTTGTTGCCACAGCCATTTTGATTTCAATGTTTCTAATAATGGCCATATTTGAACATTTGTTCAAACCCACACAACACTTTTCTTCCACGCCAGAATCTATGCTTCCAACTTATCAGGAACACCACTCACTACCAACCACAAAACAAGGAAATGCACAATCAGTTCTATCATCGTCTTATGCATGTGATTTGTCGGTGTTGATGCCGGGGCAGCAGTATCCTACTTACATTGCCCAACCTGCTCCTCTGCCTTGTCCAAGGGAAGAAGCTTATTGGCCTTCTCACCAACAACATTTTGTATTTAACAACTAG

Protein sequence

>MsG0280006310.01.T01

MSDLNSERSKMWNVYTASNAGPSHSQTGGGVDEEGPWKSFGTSMSAISFGFVATAILISMFLIMAIFEHLFKPTQHFSSTPESMLPTYQEHHSLPTTKQGNAQSVLSSSYACDLSVLMPGQQYPTYIAQPAPLPCPREEAYWPSHQQHFVFNN*