AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004451.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004451.01.T01 MTR_1g080150 94.928 138 6 1 1 137 1 138 8.76e-88 256
MsG0180004451.01.T01 MTR_1g080150 94.928 138 6 1 1 137 1 138 1.26e-87 253
MsG0180004451.01.T01 MTR_1g080150 94.928 138 6 1 1 137 1 138 2.01e-87 252
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004451.01.T01 AT3G04500 68.421 95 25 4 45 137 52 143 6.93e-35 119
MsG0180004451.01.T01 AT3G04500 72.941 85 19 3 55 137 61 143 1.92e-34 118
MsG0180004451.01.T01 AT3G04500 68.421 95 25 4 45 137 52 143 2.11e-34 120

Find 63 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGAGGAACAGCTTTCTTCT+TGG 0.222688 1:+78160515 None:intergenic
TCTAAAATCGCGTTTGGTTT+AGG 0.252268 1:+78161008 None:intergenic
TAAGCAGCAGGCGGTGGAAC+GGG 0.268956 1:+78160754 None:intergenic
GGCATTGGAATATGTGAATT+GGG 0.328527 1:+78160888 None:intergenic
CAATGTTTGATTGATCGAAT+TGG 0.343692 1:+78160979 None:intergenic
CGGAGGAAGCTGCACGGCGG+GGG 0.354638 1:+78160792 None:intergenic
CTTCCTCCGCCGCCAATTGT+CGG 0.356289 1:-78160778 MsG0180004451.01.T01:CDS
TGTGAGAGGTAGTGTGTGTT+TGG 0.374014 1:-78160348 MsG0180004451.01.T01:intron
AATGTTTGATTGATCGAATT+GGG 0.378975 1:+78160980 None:intergenic
TGTTGAAGGTGAAAGGGAAC+GGG 0.379503 1:+78160856 None:intergenic
GGCGGAGGAAGCTGCACGGC+GGG 0.390499 1:+78160790 None:intergenic
GGGCATTGGAATATGTGAAT+TGG 0.406105 1:+78160887 None:intergenic
GGTGGAACGGGGCCGACAAT+TGG 0.409141 1:+78160766 None:intergenic
GGAAAATCTAAAATCGCGTT+TGG 0.410614 1:+78161002 None:intergenic
TGAAAGGGAACGGGGAAGTA+AGG 0.412150 1:+78160865 None:intergenic
GGGCCGACAATTGGCGGCGG+AGG 0.430346 1:+78160775 None:intergenic
GCGGAGGAAGCTGCACGGCG+GGG 0.443060 1:+78160791 None:intergenic
GCGTAGTGAGAGGTGGTTGC+GGG 0.445928 1:+78160832 None:intergenic
CGGCGGAGGAAGCTGCACGG+CGG 0.453429 1:+78160789 None:intergenic
GGAACGGGGCCGACAATTGG+CGG 0.457314 1:+78160769 None:intergenic
GAAAGCTGCTGGTCAAGCTT+GGG 0.460458 1:-78160493 MsG0180004451.01.T01:CDS
AACGGGGAAGTAAGGGGCAT+TGG 0.463371 1:+78160873 None:intergenic
GGAAAGCTGCTGGTCAAGCT+TGG 0.464391 1:-78160494 MsG0180004451.01.T01:CDS
TCAGGCCACTCTGCAAGAAT+AGG 0.467965 1:+78160465 None:intergenic
TGCGGGTTGTTGAAGGTGAA+AGG 0.471510 1:+78160849 None:intergenic
GGTGGTGTCGTCATTGCAAT+TGG 0.472771 1:+78160931 None:intergenic
AGGTGGTTGCGGGTTGTTGA+AGG 0.484864 1:+78160842 None:intergenic
GCTGTTCCTCGGAAAGCTGC+TGG 0.484908 1:-78160504 MsG0180004451.01.T01:CDS
ACGGAGTAAGCAGCAGGCGG+TGG 0.487179 1:+78160748 None:intergenic
AAAGGGAACGGGGAAGTAAG+GGG 0.493709 1:+78160867 None:intergenic
GTAAGCAGCAGGCGGTGGAA+CGG 0.496822 1:+78160753 None:intergenic
GTTTGGTTTAGGTTTGTTTG+AGG 0.500810 1:+78161019 None:intergenic
TTGTTGAAGGTGAAAGGGAA+CGG 0.508796 1:+78160855 None:intergenic
GAAAGGGAACGGGGAAGTAA+GGG 0.516807 1:+78160866 None:intergenic
TGCGGAACGGAGTAAGCAGC+AGG 0.541336 1:+78160742 None:intergenic
GCGGGTTGTTGAAGGTGAAA+GGG 0.543608 1:+78160850 None:intergenic
AGCTGCTGGTCAAGCTTGGG+AGG 0.546251 1:-78160490 MsG0180004451.01.T01:CDS
TTCACATTCTCAAGAGCCTC+CGG 0.552039 1:+78160579 None:intergenic
CTCAACATCGCTACTCGCAA+TGG 0.565157 1:+78160551 None:intergenic
AAGCAGCAGGCGGTGGAACG+GGG 0.570100 1:+78160755 None:intergenic
CCAAGAAGAAAGCTGTTCCT+CGG 0.572765 1:-78160515 MsG0180004451.01.T01:CDS
GGCGTAGTGAGAGGTGGTTG+CGG 0.577840 1:+78160831 None:intergenic
GTTGAAGGTGAAAGGGAACG+GGG 0.590747 1:+78160857 None:intergenic
ATTCTTGCAGAGTGGCCTGA+AGG 0.594669 1:-78160462 MsG0180004451.01.T01:intron
AAAATACTAACTTGATATTG+CGG 0.595828 1:+78160724 None:intergenic
GCTTGACCAGCAGCTTTCCG+AGG 0.598696 1:+78160498 None:intergenic
GTAGGGTGCGGCGTAGTGAG+AGG 0.607879 1:+78160822 None:intergenic
GGGTGCGGCGTAGTGAGAGG+TGG 0.612059 1:+78160825 None:intergenic
GGCTCTTGAGAATGTGAAGG+CGG 0.612668 1:-78160574 MsG0180004451.01.T01:CDS
ACGGGGCCGACAATTGGCGG+CGG 0.614773 1:+78160772 None:intergenic
GGTTGAGATGATGAGGATGA+AGG 0.616374 1:+78160910 None:intergenic
TGGAACGAATCGTGAATCGA+TGG 0.619092 1:+78160951 None:intergenic
ATGTTTGATTGATCGAATTG+GGG 0.621330 1:+78160981 None:intergenic
TGGCGGCGGAGGAAGCTGCA+CGG 0.623515 1:+78160786 None:intergenic
GCATTGGAATATGTGAATTG+GGG 0.628373 1:+78160889 None:intergenic
GGAGGCTCTTGAGAATGTGA+AGG 0.648124 1:-78160577 MsG0180004451.01.T01:CDS
TGAGATGATGAGGATGAAGG+TGG 0.660814 1:+78160913 None:intergenic
AGGATCCTATTCTTGCAGAG+TGG 0.663088 1:-78160470 MsG0180004451.01.T01:CDS
TGCACAGATAATTACGCCGG+AGG 0.666682 1:-78160595 MsG0180004451.01.T01:CDS
GAATTGGGGTTGAGATGATG+AGG 0.677929 1:+78160903 None:intergenic
AGATGCACAGATAATTACGC+CGG 0.679077 1:-78160598 MsG0180004451.01.T01:CDS
ACTAACTTGATATTGCGGAA+CGG 0.699014 1:+78160729 None:intergenic
GGAACGGAGTAAGCAGCAGG+CGG 0.735035 1:+78160745 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAATTAATTAACGAAT+TGG + Chr1:78160977-78160996 None:intergenic 10.0%
!! TTTAATGTATATTGGAAATT+TGG - Chr1:78160995-78161014 MsG0180004451.01.T01:five_prime_UTR 15.0%
!! AAAATACTAACTTGATATTG+CGG + Chr1:78160646-78160665 None:intergenic 20.0%
!! TCAATTTGTATTTGGAAATT+GGG - Chr1:78160676-78160695 MsG0180004451.01.T01:intron 20.0%
!! TTCAATTTGTATTTGGAAAT+TGG - Chr1:78160675-78160694 MsG0180004451.01.T01:intron 20.0%
!!! TCTTCTTTTCAATTTGTATT+TGG - Chr1:78160668-78160687 MsG0180004451.01.T01:intron 20.0%
! AATGTTTGATTGATCGAATT+GGG + Chr1:78160390-78160409 None:intergenic 25.0%
ATGTTTGATTGATCGAATTG+GGG + Chr1:78160389-78160408 None:intergenic 30.0%
ATTTGATTGATTGTGTTGTG+TGG - Chr1:78160718-78160737 MsG0180004451.01.T01:intron 30.0%
CAATGTTTGATTGATCGAAT+TGG + Chr1:78160391-78160410 None:intergenic 30.0%
GAAATTGGGAAAAGAGAATT+TGG - Chr1:78160690-78160709 MsG0180004451.01.T01:intron 30.0%
ACTAACTTGATATTGCGGAA+CGG + Chr1:78160641-78160660 None:intergenic 35.0%
ATTGGAAATTTGGTTGTGAG+AGG - Chr1:78161005-78161024 MsG0180004451.01.T01:five_prime_UTR 35.0%
CATGATGATACAATACCTTC+AGG + Chr1:78160923-78160942 None:intergenic 35.0%
GCATTGGAATATGTGAATTG+GGG + Chr1:78160481-78160500 None:intergenic 35.0%
! GGAAAATCTAAAATCGCGTT+TGG + Chr1:78160368-78160387 None:intergenic 35.0%
! GGCATTGGAATATGTGAATT+GGG + Chr1:78160482-78160501 None:intergenic 35.0%
! GTTTGGTTTAGGTTTGTTTG+AGG + Chr1:78160351-78160370 None:intergenic 35.0%
! TCTAAAATCGCGTTTGGTTT+AGG + Chr1:78160362-78160381 None:intergenic 35.0%
!!! GTAGTGTGTGTTTGGTTTTT+CGG - Chr1:78161027-78161046 MsG0180004451.01.T01:five_prime_UTR 35.0%
AGATGCACAGATAATTACGC+CGG - Chr1:78160769-78160788 MsG0180004451.01.T01:CDS 40.0%
ATTGTGTTGTGTGGTTGAAC+AGG - Chr1:78160727-78160746 MsG0180004451.01.T01:intron 40.0%
TTGTTGAAGGTGAAAGGGAA+CGG + Chr1:78160515-78160534 None:intergenic 40.0%
!! GGGCATTGGAATATGTGAAT+TGG + Chr1:78160483-78160502 None:intergenic 40.0%
AGGATCCTATTCTTGCAGAG+TGG - Chr1:78160897-78160916 MsG0180004451.01.T01:CDS 45.0%
CCAAGAAGAAAGCTGTTCCT+CGG - Chr1:78160852-78160871 MsG0180004451.01.T01:CDS 45.0%
GAATTGGGGTTGAGATGATG+AGG + Chr1:78160467-78160486 None:intergenic 45.0%
GGTTGAGATGATGAGGATGA+AGG + Chr1:78160460-78160479 None:intergenic 45.0%
TGAGATGATGAGGATGAAGG+TGG + Chr1:78160457-78160476 None:intergenic 45.0%
TGGAACGAATCGTGAATCGA+TGG + Chr1:78160419-78160438 None:intergenic 45.0%
TGTGAGAGGTAGTGTGTGTT+TGG - Chr1:78161019-78161038 MsG0180004451.01.T01:five_prime_UTR 45.0%
TGTTGAAGGTGAAAGGGAAC+GGG + Chr1:78160514-78160533 None:intergenic 45.0%
TTCACATTCTCAAGAGCCTC+CGG + Chr1:78160791-78160810 None:intergenic 45.0%
!!! TAATTTATTTTAATGTATAT+TGG - Chr1:78160987-78161006 MsG0180004451.01.T01:five_prime_UTR 5.0%
AAAGGGAACGGGGAAGTAAG+GGG + Chr1:78160503-78160522 None:intergenic 50.0%
ATTCTTGCAGAGTGGCCTGA+AGG - Chr1:78160905-78160924 MsG0180004451.01.T01:CDS 50.0%
CCGAGGAACAGCTTTCTTCT+TGG + Chr1:78160855-78160874 None:intergenic 50.0%
CTCAACATCGCTACTCGCAA+TGG + Chr1:78160819-78160838 None:intergenic 50.0%
GAAAGCTGCTGGTCAAGCTT+GGG - Chr1:78160874-78160893 MsG0180004451.01.T01:CDS 50.0%
GAAAGGGAACGGGGAAGTAA+GGG + Chr1:78160504-78160523 None:intergenic 50.0%
GGAGGCTCTTGAGAATGTGA+AGG - Chr1:78160790-78160809 MsG0180004451.01.T01:CDS 50.0%
GGCTCTTGAGAATGTGAAGG+CGG - Chr1:78160793-78160812 MsG0180004451.01.T01:CDS 50.0%
GTTGAAGGTGAAAGGGAACG+GGG + Chr1:78160513-78160532 None:intergenic 50.0%
TCAGGCCACTCTGCAAGAAT+AGG + Chr1:78160905-78160924 None:intergenic 50.0%
TGAAAGGGAACGGGGAAGTA+AGG + Chr1:78160505-78160524 None:intergenic 50.0%
TGCACAGATAATTACGCCGG+AGG - Chr1:78160772-78160791 MsG0180004451.01.T01:CDS 50.0%
! GCGGGTTGTTGAAGGTGAAA+GGG + Chr1:78160520-78160539 None:intergenic 50.0%
! TGCGGGTTGTTGAAGGTGAA+AGG + Chr1:78160521-78160540 None:intergenic 50.0%
!! GGTGGTGTCGTCATTGCAAT+TGG + Chr1:78160439-78160458 None:intergenic 50.0%
GGAAAGCTGCTGGTCAAGCT+TGG - Chr1:78160873-78160892 MsG0180004451.01.T01:CDS 55.0%
! AACGGGGAAGTAAGGGGCAT+TGG + Chr1:78160497-78160516 None:intergenic 55.0%
! AGGTGGTTGCGGGTTGTTGA+AGG + Chr1:78160528-78160547 None:intergenic 55.0%
AGCTGCTGGTCAAGCTTGGG+AGG - Chr1:78160877-78160896 MsG0180004451.01.T01:CDS 60.0%
CTTCCTCCGCCGCCAATTGT+CGG - Chr1:78160589-78160608 MsG0180004451.01.T01:CDS 60.0%
GCGTAGTGAGAGGTGGTTGC+GGG + Chr1:78160538-78160557 None:intergenic 60.0%
GCTGTTCCTCGGAAAGCTGC+TGG - Chr1:78160863-78160882 MsG0180004451.01.T01:CDS 60.0%
GCTTGACCAGCAGCTTTCCG+AGG + Chr1:78160872-78160891 None:intergenic 60.0%
GGAACGGAGTAAGCAGCAGG+CGG + Chr1:78160625-78160644 None:intergenic 60.0%
GGCGTAGTGAGAGGTGGTTG+CGG + Chr1:78160539-78160558 None:intergenic 60.0%
GTAAGCAGCAGGCGGTGGAA+CGG + Chr1:78160617-78160636 None:intergenic 60.0%
TAAGCAGCAGGCGGTGGAAC+GGG + Chr1:78160616-78160635 None:intergenic 60.0%
TGCGGAACGGAGTAAGCAGC+AGG + Chr1:78160628-78160647 None:intergenic 60.0%
AAGCAGCAGGCGGTGGAACG+GGG + Chr1:78160615-78160634 None:intergenic 65.0%
ACGGAGTAAGCAGCAGGCGG+TGG + Chr1:78160622-78160641 None:intergenic 65.0%
GGAACGGGGCCGACAATTGG+CGG + Chr1:78160601-78160620 None:intergenic 65.0%
GGTGGAACGGGGCCGACAAT+TGG + Chr1:78160604-78160623 None:intergenic 65.0%
GTAGGGTGCGGCGTAGTGAG+AGG + Chr1:78160548-78160567 None:intergenic 65.0%
ACGGGGCCGACAATTGGCGG+CGG + Chr1:78160598-78160617 None:intergenic 70.0%
GGGTGCGGCGTAGTGAGAGG+TGG + Chr1:78160545-78160564 None:intergenic 70.0%
TGGCGGCGGAGGAAGCTGCA+CGG + Chr1:78160584-78160603 None:intergenic 70.0%
ACGGCGGGGGTGGCGACGTA+GGG + Chr1:78160565-78160584 None:intergenic 75.0%
AGGAAGCTGCACGGCGGGGG+TGG + Chr1:78160575-78160594 None:intergenic 75.0%
CGGAGGAAGCTGCACGGCGG+GGG + Chr1:78160578-78160597 None:intergenic 75.0%
CGGCGGAGGAAGCTGCACGG+CGG + Chr1:78160581-78160600 None:intergenic 75.0%
GCGGAGGAAGCTGCACGGCG+GGG + Chr1:78160579-78160598 None:intergenic 75.0%
GGCGGAGGAAGCTGCACGGC+GGG + Chr1:78160580-78160599 None:intergenic 75.0%
GGGCCGACAATTGGCGGCGG+AGG + Chr1:78160595-78160614 None:intergenic 75.0%
GGGGGTGGCGACGTAGGGTG+CGG + Chr1:78160560-78160579 None:intergenic 75.0%
! CACGGCGGGGGTGGCGACGT+AGG + Chr1:78160566-78160585 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr1 gene 78160327 78161062 78160327 ID=MsG0180004451.01;Name=MsG0180004451.01
Chr1 mRNA 78160327 78161062 78160327 ID=MsG0180004451.01.T01;Parent=MsG0180004451.01;Name=MsG0180004451.01.T01;_AED=0.48;_eAED=0.51;_QI=118|0.5|0.66|1|1|1|3|0|139
Chr1 exon 78160327 78160358 78160327 ID=MsG0180004451.01.T01:exon:45288;Parent=MsG0180004451.01.T01
Chr1 exon 78160463 78160640 78160463 ID=MsG0180004451.01.T01:exon:45287;Parent=MsG0180004451.01.T01
Chr1 exon 78160735 78161062 78160735 ID=MsG0180004451.01.T01:exon:45286;Parent=MsG0180004451.01.T01
Chr1 five_prime_UTR 78160945 78161062 78160945 ID=MsG0180004451.01.T01:five_prime_utr;Parent=MsG0180004451.01.T01
Chr1 CDS 78160735 78160944 78160735 ID=MsG0180004451.01.T01:cds;Parent=MsG0180004451.01.T01
Chr1 CDS 78160463 78160640 78160463 ID=MsG0180004451.01.T01:cds;Parent=MsG0180004451.01.T01
Chr1 CDS 78160327 78160358 78160327 ID=MsG0180004451.01.T01:cds;Parent=MsG0180004451.01.T01
Gene Sequence

>MsG0180004451.01.T01

ATGACGACACCACCTTCATCCTCATCATCTCAACCCCAATTCACATATTCCAATGCCCCTTACTTCCCCGTTCCCTTTCACCTTCAACAACCCGCAACCACCTCTCACTACGCCGCACCCTACGTCGCCACCCCCGCCGTGCAGCTTCCTCCGCCGCCAATTGTCGGCCCCGTTCCACCGCCTGCTGCTTACTCCGTTCCGCAATATCAAGCACAGCAATTGTTTGAGAGAGATGCACAGATAATTACGCCGGAGGCTCTTGAGAATGTGAAGGCGGCCATTGCGAGTAGCGATGTTGAGCATAAAGCTGAGACCAAGAAGAAAGCTGTTCCTCGGAAAGCTGCTGGTCAAGCTTGGGAGGATCCTATTCTTGCAGAGTGGCCTGAAGTGTGTGTTTGGTTTTTCGGTGACAAAGATTGA

Protein sequence

>MsG0180004451.01.T01

MTTPPSSSSSQPQFTYSNAPYFPVPFHLQQPATTSHYAAPYVATPAVQLPPPPIVGPVPPPAAYSVPQYQAQQLFERDAQIITPEALENVKAAIASSDVEHKAETKKKAVPRKAAGQAWEDPILAEWPEVCVWFFGDKD*