AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040127.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040127.01.T01 MTR_7g084890 93.991 233 4 3 13 235 1 233 2.53e-159 441
MsG0780040127.01.T01 MTR_1g116580 43.216 199 101 3 30 225 14 203 7.06e-45 150
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040127.01.T01 AT2G31600 42.972 249 110 5 5 226 44 287 1.72e-62 197
MsG0780040127.01.T01 AT2G31600 42.972 249 110 5 5 226 44 287 1.72e-62 197
MsG0780040127.01.T01 AT2G31600 42.972 249 110 5 5 226 44 287 1.72e-62 197
MsG0780040127.01.T01 AT2G31600 42.972 249 110 5 5 226 44 287 1.72e-62 197
MsG0780040127.01.T01 AT3G53860 45.872 218 105 3 20 226 52 267 2.18e-61 194
MsG0780040127.01.T01 AT1G05860 45.106 235 108 3 13 226 32 266 5.32e-59 188
MsG0780040127.01.T01 AT1G05860 45.106 235 108 3 13 226 32 266 5.32e-59 188
MsG0780040127.01.T01 AT1G05860 45.143 175 70 4 5 153 19 193 1.46e-36 129
MsG0780040127.01.T01 AT2G31600 41.714 175 70 5 5 152 44 213 1.05e-35 126
MsG0780040127.01.T01 AT2G31600 41.714 175 70 5 5 152 44 213 1.05e-35 126

Find 69 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTGCTTCGTGCTGTAAAA+AGG 0.184649 7:+73351807 MsG0780040127.01.T01:CDS
TGAATTTGGCGGACAAATTC+AGG 0.235095 7:-73351887 None:intergenic
GCTGTTACCGAGGTCATTAC+TGG 0.271359 7:+73349815 MsG0780040127.01.T01:CDS
ATTGGTGGTGGTGGATTTAT+TGG 0.277786 7:-73349646 None:intergenic
AGGTCATTACTGGGCGTTAA+TGG 0.298106 7:+73349825 MsG0780040127.01.T01:CDS
GGTTAATCAGTCTCAGGTTT+AGG 0.308564 7:-73351944 None:intergenic
GGACTAGGCTTACCATTAAC+TGG 0.312740 7:-73351845 None:intergenic
GCTTTATGCAGTTTGCCAAC+AGG 0.332413 7:+73351704 MsG0780040127.01.T01:intron
GGTCATAACGTCCCAGTTAA+TGG 0.342126 7:+73351833 MsG0780040127.01.T01:CDS
GGAAGATGAAGAAAGAAATT+CGG 0.342169 7:+73349906 MsG0780040127.01.T01:CDS
CTAAGTACAGAGAGTATTAC+TGG 0.348566 7:+73349860 MsG0780040127.01.T01:CDS
GTTGCACCGCCGTGGCAAAA+AGG 0.349754 7:+73350067 MsG0780040127.01.T01:CDS
AGAGAGTATTACTGGACTTA+TGG 0.365244 7:+73349868 MsG0780040127.01.T01:CDS
ATTGAGATCCATGGTTCCTT+TGG 0.385033 7:+73351751 MsG0780040127.01.T01:CDS
GCTTCTGCTGCTGTTGATAA+TGG 0.392369 7:+73349934 MsG0780040127.01.T01:CDS
GAAGATGAAGAAAGAAATTC+GGG 0.400485 7:+73349907 MsG0780040127.01.T01:CDS
ATCTTTGGTTAATCAGTCTC+AGG 0.414837 7:-73351950 None:intergenic
CCACGATAAAGCTTCTGATT+AGG 0.415729 7:-73350045 None:intergenic
CACGGCGGAGGCGGCGGACG+AGG 0.423786 7:-73349777 None:intergenic
GTCTGACACGGCGGAGGCGG+CGG 0.433953 7:-73349783 None:intergenic
GAGTCTTCCATTGGTGGTGG+TGG 0.438608 7:-73349655 None:intergenic
AATAGATGTAACTGCGGACT+AGG 0.448722 7:-73351860 None:intergenic
GACCTCGCGGCGGGTTAAAA+CGG 0.448768 7:-73349755 None:intergenic
TCGCCTAATTGGTAATGAGC+AGG 0.451526 7:-73351779 None:intergenic
GGAACCATGGATCTCAATAC+TGG 0.454340 7:-73351746 None:intergenic
TGCCCTGCTCATTACCAATT+AGG 0.459872 7:+73351776 MsG0780040127.01.T01:CDS
TGCTGTTGAGAGTCTTCCAT+TGG 0.466609 7:-73349664 None:intergenic
CTTTCTTCATCTTCCTTGAA+CGG 0.468778 7:-73349898 None:intergenic
GTTGTCTGACACGGCGGAGG+CGG 0.468951 7:-73349786 None:intergenic
GGCGTTAATGGAAGAGCTTA+AGG 0.485899 7:+73349837 MsG0780040127.01.T01:CDS
TAAACGAAAAGCTGCGCGCA+AGG 0.489819 7:+73351913 MsG0780040127.01.T01:CDS
ATAATGGTGATGACTTTGTT+CGG 0.491789 7:+73349950 MsG0780040127.01.T01:CDS
TGTTGAGAGTCTTCCATTGG+TGG 0.497279 7:-73349661 None:intergenic
GCGGACGAGGACCTCGCGGC+GGG 0.498422 7:-73349764 None:intergenic
CGCCTAATTGGTAATGAGCA+GGG 0.500265 7:-73351778 None:intergenic
CTTTATGCAGTTTGCCAACA+GGG 0.500980 7:+73351705 MsG0780040127.01.T01:intron
TTATGGAAGGAGTCCGTTCA+AGG 0.519406 7:+73349885 MsG0780040127.01.T01:CDS
TCGGCACCGTCGATTGTCAT+CGG 0.530875 7:-73349706 None:intergenic
AATAAATCCACCACCACCAA+TGG 0.548677 7:+73349648 MsG0780040127.01.T01:CDS
GTCATCGGCGCGATTTGTTG+CGG 0.555912 7:-73349691 None:intergenic
ACTGGCTTATTACAAAAGAC+TGG 0.557434 7:-73351728 None:intergenic
GACTAGGCTTACCATTAACT+GGG 0.570203 7:-73351844 None:intergenic
TGAGAGTCTTCCATTGGTGG+TGG 0.572325 7:-73349658 None:intergenic
TTACAAAAGACTGGCCCTGT+TGG 0.574396 7:-73351719 None:intergenic
CTGTTACCGAGGTCATTACT+GGG 0.578965 7:+73349816 MsG0780040127.01.T01:CDS
CTTAGTAAGAGCCATAGCCT+TGG 0.581621 7:-73349995 None:intergenic
TTTGTTCGGTGTGCTTCTAG+TGG 0.584923 7:+73349964 MsG0780040127.01.T01:CDS
AGAGTTGTCTGACACGGCGG+AGG 0.587249 7:-73349789 None:intergenic
TGGTTGCAAGACCAAGGCTA+TGG 0.587740 7:+73349984 MsG0780040127.01.T01:CDS
CAACTCTCTCGCTGTTACCG+AGG 0.590857 7:+73349805 MsG0780040127.01.T01:CDS
AGTATTACTGGACTTATGGA+AGG 0.608751 7:+73349872 MsG0780040127.01.T01:CDS
GGCGGACGAGGACCTCGCGG+CGG 0.609662 7:-73349765 None:intergenic
TAAGCCAGTATTGAGATCCA+TGG 0.612741 7:+73351742 MsG0780040127.01.T01:CDS
AGCGAGGGCTAAGTCTTCGT+CGG 0.616825 7:-73349725 None:intergenic
TTATCGTGGTTGCACCGCCG+TGG 0.625527 7:+73350059 MsG0780040127.01.T01:CDS
TTCTAGTGGTTGCAAGACCA+AGG 0.627196 7:+73349978 MsG0780040127.01.T01:CDS
ATCGGCGCGATTTGTTGCGG+TGG 0.628254 7:-73349688 None:intergenic
GGCGGCGGACGAGGACCTCG+CGG 0.628435 7:-73349768 None:intergenic
TAATGAGCAGGGCATACCAA+AGG 0.651701 7:-73351767 None:intergenic
CTCGCGGCGGGTTAAAACGG+AGG 0.662601 7:-73349752 None:intergenic
ATCGCGCCGATGACAATCGA+CGG 0.665199 7:+73349700 MsG0780040127.01.T01:CDS
CCTAATCAGAAGCTTTATCG+TGG 0.666724 7:+73350045 MsG0780040127.01.T01:CDS
TTAAAACGGAGGAGTTAGCG+AGG 0.671046 7:-73349741 None:intergenic
AGGGCATACCAAAGGAACCA+TGG 0.672546 7:-73351759 None:intergenic
AGCGAGAGAGTTGTCTGACA+CGG 0.684863 7:-73349795 None:intergenic
TAAAACGGAGGAGTTAGCGA+GGG 0.688548 7:-73349740 None:intergenic
GAGAGAGTTGTCTGACACGG+CGG 0.692611 7:-73349792 None:intergenic
GGAATTAATAGATGTAACTG+CGG 0.701946 7:-73351866 None:intergenic
TTAACGCCCAGTAATGACCT+CGG 0.702952 7:-73349822 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAATTTTTCAAATTATTAT+CGG - Chr7:73350622-73350641 None:intergenic 10.0%
!!! TTTTTGATTAATAACAAATA+TGG + Chr7:73350190-73350209 MsG0780040127.01.T01:intron 10.0%
!! AAGCATTAAATTTAATTAGT+AGG - Chr7:73351533-73351552 None:intergenic 15.0%
!! TTAAATTTAATTAGTAGGAT+AGG - Chr7:73351528-73351547 None:intergenic 15.0%
!!! ATACTATTATTTTTTTTGAC+TGG + Chr7:73350315-73350334 MsG0780040127.01.T01:intron 15.0%
!!! TACTATTATTTTTTTTGACT+GGG + Chr7:73350316-73350335 MsG0780040127.01.T01:intron 15.0%
!!! TTTGTTTGTTTATATGTTTT+TGG + Chr7:73350393-73350412 MsG0780040127.01.T01:intron 15.0%
!! AAAAATTGTATAATGCAAGA+TGG - Chr7:73351459-73351478 None:intergenic 20.0%
!! AGTTATAATTGTAATTCAAC+AGG + Chr7:73350989-73351008 MsG0780040127.01.T01:intron 20.0%
!!! ACAATTTTTGGTTTTTATAC+AGG + Chr7:73351470-73351489 MsG0780040127.01.T01:intron 20.0%
!!! ATCTTGCATTATACAATTTT+TGG + Chr7:73351458-73351477 MsG0780040127.01.T01:intron 20.0%
!!! ATTTGGTAATTTGTGTTTTT+AGG + Chr7:73350231-73350250 MsG0780040127.01.T01:intron 20.0%
!!! CTTTTCGTTTATTTTGAATT+TGG - Chr7:73351904-73351923 None:intergenic 20.0%
!!! GAAGAAGATAATGTTTTTTT+TGG + Chr7:73350269-73350288 MsG0780040127.01.T01:intron 20.0%
!!! TTTGGTAATTTGTGTTTTTA+GGG + Chr7:73350232-73350251 MsG0780040127.01.T01:intron 20.0%
! CACAAAACATATAGATTGAA+TGG + Chr7:73350876-73350895 MsG0780040127.01.T01:intron 25.0%
! GTAATACATGAAAACAGAAA+AGG - Chr7:73351619-73351638 None:intergenic 25.0%
! TAATTGTGTTTCTTGCAATT+TGG + Chr7:73350214-73350233 MsG0780040127.01.T01:intron 25.0%
!! CAATGTGATCATATATGTTT+TGG + Chr7:73350508-73350527 MsG0780040127.01.T01:intron 25.0%
!! GTTCAAAATGTTTTTCCTTT+CGG + Chr7:73350789-73350808 MsG0780040127.01.T01:intron 25.0%
!!! CTACTTTTTTCTCTTGTTTT+TGG + Chr7:73350823-73350842 MsG0780040127.01.T01:intron 25.0%
!!! TACTGTATTGTTTTTGATGA+TGG + Chr7:73350147-73350166 MsG0780040127.01.T01:intron 25.0%
!!! TATTTTTTTTGACTGGGTTT+TGG + Chr7:73350322-73350341 MsG0780040127.01.T01:intron 25.0%
!!! TTCGTTTATTTTGAATTTGG+CGG - Chr7:73351901-73351920 None:intergenic 25.0%
!!! TTTTTTTGGATTTTCTTGTG+TGG + Chr7:73350283-73350302 MsG0780040127.01.T01:intron 25.0%
AAAAGGTCCAGGTTATTATT+CGG - Chr7:73351085-73351104 None:intergenic 30.0%
AATTGTAATTCAACAGGTGT+GGG + Chr7:73350995-73351014 MsG0780040127.01.T01:intron 30.0%
ACATAAACACTTGTAGTCTA+TGG + Chr7:73350851-73350870 MsG0780040127.01.T01:intron 30.0%
ATGCAGTGTACATGTTAAAA+GGG + Chr7:73351402-73351421 MsG0780040127.01.T01:intron 30.0%
ATTACCTACAGAAGACAATT+CGG + Chr7:73351667-73351686 MsG0780040127.01.T01:intron 30.0%
ATTGACATAATTCAACGTGA+TGG + Chr7:73350637-73350656 MsG0780040127.01.T01:intron 30.0%
CATGAACTGAATAAAGATTG+AGG - Chr7:73351059-73351078 None:intergenic 30.0%
CCTTAATACTTACATCTCAA+GGG - Chr7:73351266-73351285 None:intergenic 30.0%
CCTTGAGATGTAAGTATTAA+GGG + Chr7:73351264-73351283 MsG0780040127.01.T01:intron 30.0%
CTCAATCTTTATTCAGTTCA+TGG + Chr7:73351057-73351076 MsG0780040127.01.T01:intron 30.0%
GAAGATGAAGAAAGAAATTC+GGG + Chr7:73349907-73349926 MsG0780040127.01.T01:CDS 30.0%
GATAGAAACTTGGGTATTAT+AGG + Chr7:73351175-73351194 MsG0780040127.01.T01:intron 30.0%
GGAAGATGAAGAAAGAAATT+CGG + Chr7:73349906-73349925 MsG0780040127.01.T01:CDS 30.0%
GGAATTAATAGATGTAACTG+CGG - Chr7:73351869-73351888 None:intergenic 30.0%
TAATTGTAATTCAACAGGTG+TGG + Chr7:73350994-73351013 MsG0780040127.01.T01:intron 30.0%
! AACAGATATGTATTGATGCT+TGG - Chr7:73351360-73351379 None:intergenic 30.0%
! AATAATAACCTGGACCTTTT+TGG + Chr7:73351085-73351104 MsG0780040127.01.T01:intron 30.0%
! ACATTGTCAATCTGAAATGT+TGG + Chr7:73350951-73350970 MsG0780040127.01.T01:intron 30.0%
! ATAATGGTGATGACTTTGTT+CGG + Chr7:73349950-73349969 MsG0780040127.01.T01:CDS 30.0%
! GGATTTATTGGAGAGATTTT+CGG - Chr7:73349637-73349656 None:intergenic 30.0%
! TGTTGTTGTTCTTGACATAT+TGG + Chr7:73350547-73350566 MsG0780040127.01.T01:intron 30.0%
!! TTGTGGTTGATGAGAAAAAT+GGG + Chr7:73350366-73350385 MsG0780040127.01.T01:intron 30.0%
AAAGGTCCAGGTTATTATTC+GGG - Chr7:73351084-73351103 None:intergenic 35.0%
ACAACAACACTTCTGATTGA+AGG - Chr7:73350536-73350555 None:intergenic 35.0%
AGGATGACAAGATTGAAAGA+AGG - Chr7:73351149-73351168 None:intergenic 35.0%
AGTATTACTGGACTTATGGA+AGG + Chr7:73349872-73349891 MsG0780040127.01.T01:CDS 35.0%
CAACTTTCTCAGACTATTAC+AGG - Chr7:73350482-73350501 None:intergenic 35.0%
CAAGAACTTCGAATCAAACT+TGG - Chr7:73351111-73351130 None:intergenic 35.0%
CATATAGATTGAATGGAACC+AGG + Chr7:73350883-73350902 MsG0780040127.01.T01:intron 35.0%
CATGCAGTGTACATGTTAAA+AGG + Chr7:73351401-73351420 MsG0780040127.01.T01:intron 35.0%
CCCTTAATACTTACATCTCA+AGG - Chr7:73351267-73351286 None:intergenic 35.0%
CCCTTGAGATGTAAGTATTA+AGG + Chr7:73351263-73351282 MsG0780040127.01.T01:intron 35.0%
CTTTCTTCATCTTCCTTGAA+CGG - Chr7:73349901-73349920 None:intergenic 35.0%
! AAAAACTCACCTTTTTGCCA+CGG - Chr7:73350079-73350098 None:intergenic 35.0%
! ACTGGCTTATTACAAAAGAC+TGG - Chr7:73351731-73351750 None:intergenic 35.0%
! ATGTATTGATGCTTGGAAGT+TGG - Chr7:73351353-73351372 None:intergenic 35.0%
! CAATTCGGCTGATAAACTTT+TGG + Chr7:73351682-73351701 MsG0780040127.01.T01:intron 35.0%
! CCTTGCTATGATAGAAACTT+GGG + Chr7:73351166-73351185 MsG0780040127.01.T01:intron 35.0%
! TCCTTGCTATGATAGAAACT+TGG + Chr7:73351165-73351184 MsG0780040127.01.T01:intron 35.0%
!! AGAGAGTATTACTGGACTTA+TGG + Chr7:73349868-73349887 MsG0780040127.01.T01:CDS 35.0%
!! CTAAGTACAGAGAGTATTAC+TGG + Chr7:73349860-73349879 MsG0780040127.01.T01:CDS 35.0%
!! GGAATAATGAGTATTGACTG+TGG + Chr7:73350343-73350362 MsG0780040127.01.T01:intron 35.0%
!! GTTGTGGTTGATGAGAAAAA+TGG + Chr7:73350365-73350384 MsG0780040127.01.T01:intron 35.0%
!!! GTAGTCATGTTTTAACCGAA+AGG - Chr7:73350807-73350826 None:intergenic 35.0%
AATAAATCCACCACCACCAA+TGG + Chr7:73349648-73349667 MsG0780040127.01.T01:CDS 40.0%
AATAGATGTAACTGCGGACT+AGG - Chr7:73351863-73351882 None:intergenic 40.0%
AATTCAACGTGATGGCATGT+CGG + Chr7:73350645-73350664 MsG0780040127.01.T01:intron 40.0%
ATGCCACACAACTAAACTGA+AGG - Chr7:73351384-73351403 None:intergenic 40.0%
ATTGAGATCCATGGTTCCTT+TGG + Chr7:73351751-73351770 MsG0780040127.01.T01:CDS 40.0%
CCACGATAAAGCTTCTGATT+AGG - Chr7:73350048-73350067 None:intergenic 40.0%
CCCAAGTTTCTATCATAGCA+AGG - Chr7:73351169-73351188 None:intergenic 40.0%
CGAATCAAACTTGGCCAAAA+AGG - Chr7:73351102-73351121 None:intergenic 40.0%
CTAACATGTATCAGTGTACC+TGG - Chr7:73350904-73350923 None:intergenic 40.0%
CTTGCTTCGTGCTGTAAAAA+GGG + Chr7:73351808-73351827 MsG0780040127.01.T01:CDS 40.0%
CTTTATGCAGTTTGCCAACA+GGG + Chr7:73351705-73351724 MsG0780040127.01.T01:intron 40.0%
GACTAGGCTTACCATTAACT+GGG - Chr7:73351847-73351866 None:intergenic 40.0%
TGTGTCTAGTGTCTGAATCA+TGG - Chr7:73350934-73350953 None:intergenic 40.0%
TTGCCTTCAGTTTAGTTGTG+TGG + Chr7:73351378-73351397 MsG0780040127.01.T01:intron 40.0%
! CCTAATCAGAAGCTTTATCG+TGG + Chr7:73350045-73350064 MsG0780040127.01.T01:CDS 40.0%
! GAGAATTTTCCAAGTGCTTG+GGG + Chr7:73351286-73351305 MsG0780040127.01.T01:intron 40.0%
! GCAAGCATTTTTCGCCTAAT+TGG - Chr7:73351793-73351812 None:intergenic 40.0%
! GGAGAATTTTCCAAGTGCTT+GGG + Chr7:73351285-73351304 MsG0780040127.01.T01:intron 40.0%
! TAAGCCAGTATTGAGATCCA+TGG + Chr7:73351742-73351761 MsG0780040127.01.T01:CDS 40.0%
! TGAATTTGGCGGACAAATTC+AGG - Chr7:73351890-73351909 None:intergenic 40.0%
! TGGATTTTCTTGTGTGGCTT+TGG + Chr7:73350289-73350308 MsG0780040127.01.T01:intron 40.0%
!! ATGAGTATTGACTGTGGTTG+TGG + Chr7:73350349-73350368 MsG0780040127.01.T01:intron 40.0%
!! ATTGGTGGTGGTGGATTTAT+TGG - Chr7:73349649-73349668 None:intergenic 40.0%
AAACTTGGCCAAAAAGGTCC+AGG - Chr7:73351096-73351115 None:intergenic 45.0%
AAGTAGGATGAGATGCTCGT+TGG + Chr7:73351216-73351235 MsG0780040127.01.T01:intron 45.0%
AGGTCATTACTGGGCGTTAA+TGG + Chr7:73349825-73349844 MsG0780040127.01.T01:CDS 45.0%
AGTAGGATAGGTACAGACAG+AGG - Chr7:73351516-73351535 None:intergenic 45.0%
ATGGCTCATTCACATTGGTG+AGG - Chr7:73351440-73351459 None:intergenic 45.0%
CATCTCATCCTACTTGACGT+GGG - Chr7:73351211-73351230 None:intergenic 45.0%
CATGGACCCGAATAATAACC+TGG + Chr7:73351075-73351094 MsG0780040127.01.T01:intron 45.0%
CTGTTACCGAGGTCATTACT+GGG + Chr7:73349816-73349835 MsG0780040127.01.T01:CDS 45.0%
CTTAGTAAGAGCCATAGCCT+TGG - Chr7:73349998-73350017 None:intergenic 45.0%
GCAAGATGGCTCATTCACAT+TGG - Chr7:73351445-73351464 None:intergenic 45.0%
GCTTGCTTCGTGCTGTAAAA+AGG + Chr7:73351807-73351826 MsG0780040127.01.T01:CDS 45.0%
GCTTTATGCAGTTTGCCAAC+AGG + Chr7:73351704-73351723 MsG0780040127.01.T01:intron 45.0%
GGAACCATGGATCTCAATAC+TGG - Chr7:73351749-73351768 None:intergenic 45.0%
GGACTAGGCTTACCATTAAC+TGG - Chr7:73351848-73351867 None:intergenic 45.0%
GGCGTTAATGGAAGAGCTTA+AGG + Chr7:73349837-73349856 MsG0780040127.01.T01:CDS 45.0%
GGTCATAACGTCCCAGTTAA+TGG + Chr7:73351833-73351852 MsG0780040127.01.T01:CDS 45.0%
TAAAACGGAGGAGTTAGCGA+GGG - Chr7:73349743-73349762 None:intergenic 45.0%
TAATGAGCAGGGCATACCAA+AGG - Chr7:73351770-73351789 None:intergenic 45.0%
TCAGCCGAATTGTCTTCTGT+AGG - Chr7:73351674-73351693 None:intergenic 45.0%
TCTAGTGTCTGAATCATGGC+TGG - Chr7:73350930-73350949 None:intergenic 45.0%
TGCCCTGCTCATTACCAATT+AGG + Chr7:73351776-73351795 MsG0780040127.01.T01:CDS 45.0%
TGCTGTTGAGAGTCTTCCAT+TGG - Chr7:73349667-73349686 None:intergenic 45.0%
TGCTTGGAAGTTGGAAGTCA+TGG - Chr7:73351344-73351363 None:intergenic 45.0%
TGTTGAGAGTCTTCCATTGG+TGG - Chr7:73349664-73349683 None:intergenic 45.0%
TTAAAACGGAGGAGTTAGCG+AGG - Chr7:73349744-73349763 None:intergenic 45.0%
TTAACGCCCAGTAATGACCT+CGG - Chr7:73349825-73349844 None:intergenic 45.0%
TTATGGAAGGAGTCCGTTCA+AGG + Chr7:73349885-73349904 MsG0780040127.01.T01:CDS 45.0%
TTCTAGTGGTTGCAAGACCA+AGG + Chr7:73349978-73349997 MsG0780040127.01.T01:CDS 45.0%
! GCTTCTGCTGCTGTTGATAA+TGG + Chr7:73349934-73349953 MsG0780040127.01.T01:CDS 45.0%
! GGGAGAATTTTCCAAGTGCT+TGG + Chr7:73351284-73351303 MsG0780040127.01.T01:intron 45.0%
! TTACAAAAGACTGGCCCTGT+TGG - Chr7:73351722-73351741 None:intergenic 45.0%
!! CGCCTAATTGGTAATGAGCA+GGG - Chr7:73351781-73351800 None:intergenic 45.0%
!! GAGAAGCACTGACAAAGACA+CGG + Chr7:73350429-73350448 MsG0780040127.01.T01:intron 45.0%
!! TCGCCTAATTGGTAATGAGC+AGG - Chr7:73351782-73351801 None:intergenic 45.0%
!! TTTGTTCGGTGTGCTTCTAG+TGG + Chr7:73349964-73349983 MsG0780040127.01.T01:CDS 45.0%
!!! AATTTAATTTATTGATTTTT+TGG + Chr7:73350121-73350140 MsG0780040127.01.T01:intron 5.0%
ACGGGACATGTCTAATCGGT+GGG + Chr7:73350676-73350695 MsG0780040127.01.T01:intron 50.0%
AGCGAGAGAGTTGTCTGACA+CGG - Chr7:73349798-73349817 None:intergenic 50.0%
AGGGCATACCAAAGGAACCA+TGG - Chr7:73351762-73351781 None:intergenic 50.0%
CACTGACAAAGACACGGACA+CGG + Chr7:73350435-73350454 MsG0780040127.01.T01:intron 50.0%
CTTCGTGCTGTAAAAAGGGC+AGG + Chr7:73351812-73351831 MsG0780040127.01.T01:CDS 50.0%
GCATCTCATCCTACTTGACG+TGG - Chr7:73351212-73351231 None:intergenic 50.0%
GCTGTTACCGAGGTCATTAC+TGG + Chr7:73349815-73349834 MsG0780040127.01.T01:CDS 50.0%
GGACACGGGACATGTCTAAT+CGG + Chr7:73350672-73350691 MsG0780040127.01.T01:intron 50.0%
TAAACGAAAAGCTGCGCGCA+AGG + Chr7:73351913-73351932 MsG0780040127.01.T01:CDS 50.0%
TCCTACTTGACGTGGGACTT+AGG - Chr7:73351204-73351223 None:intergenic 50.0%
TGAGAGTCTTCCATTGGTGG+TGG - Chr7:73349661-73349680 None:intergenic 50.0%
TGATAAGCACCCCAAGCACT+TGG - Chr7:73351298-73351317 None:intergenic 50.0%
TGGTTGCAAGACCAAGGCTA+TGG + Chr7:73349984-73350003 MsG0780040127.01.T01:CDS 50.0%
! AACTCACCTTTTTGCCACGG+CGG - Chr7:73350076-73350095 None:intergenic 50.0%
ACGTGATGGCATGTCGGTGT+CGG + Chr7:73350651-73350670 MsG0780040127.01.T01:intron 55.0%
AGCGAGGGCTAAGTCTTCGT+CGG - Chr7:73349728-73349747 None:intergenic 55.0%
CAACTCTCTCGCTGTTACCG+AGG + Chr7:73349805-73349824 MsG0780040127.01.T01:CDS 55.0%
CACGGGACATGTCTAATCGG+TGG + Chr7:73350675-73350694 MsG0780040127.01.T01:intron 55.0%
CGGGACATGTCTAATCGGTG+GGG + Chr7:73350677-73350696 MsG0780040127.01.T01:intron 55.0%
GAGAGAGTTGTCTGACACGG+CGG - Chr7:73349795-73349814 None:intergenic 55.0%
GCCTAAGTCCCACGTCAAGT+AGG + Chr7:73351200-73351219 MsG0780040127.01.T01:intron 55.0%
! ATCGCGCCGATGACAATCGA+CGG + Chr7:73349700-73349719 MsG0780040127.01.T01:CDS 55.0%
! TCGGCACCGTCGATTGTCAT+CGG - Chr7:73349709-73349728 None:intergenic 55.0%
!! GAGTCTTCCATTGGTGGTGG+TGG - Chr7:73349658-73349677 None:intergenic 55.0%
!! GTCATCGGCGCGATTTGTTG+CGG - Chr7:73349694-73349713 None:intergenic 55.0%
AGAGTTGTCTGACACGGCGG+AGG - Chr7:73349792-73349811 None:intergenic 60.0%
GTTGCACCGCCGTGGCAAAA+AGG + Chr7:73350067-73350086 MsG0780040127.01.T01:CDS 60.0%
TTATCGTGGTTGCACCGCCG+TGG + Chr7:73350059-73350078 MsG0780040127.01.T01:CDS 60.0%
! GTGGGGCGTCTGTGCTTCAT+AGG + Chr7:73350694-73350713 MsG0780040127.01.T01:intron 60.0%
!! ATCGGCGCGATTTGTTGCGG+TGG - Chr7:73349691-73349710 None:intergenic 60.0%
!! GACCTCGCGGCGGGTTAAAA+CGG - Chr7:73349758-73349777 None:intergenic 60.0%
!! TGGCATGTCGGTGTCGGACA+CGG + Chr7:73350657-73350676 MsG0780040127.01.T01:intron 60.0%
GTTGTCTGACACGGCGGAGG+CGG - Chr7:73349789-73349808 None:intergenic 65.0%
!! CTCCGTTTTAACCCGCCGCG+AGG + Chr7:73349753-73349772 MsG0780040127.01.T01:CDS 65.0%
!! CTCGCGGCGGGTTAAAACGG+AGG - Chr7:73349755-73349774 None:intergenic 65.0%
!! GGCATGTCGGTGTCGGACAC+GGG + Chr7:73350658-73350677 MsG0780040127.01.T01:intron 65.0%
GTCTGACACGGCGGAGGCGG+CGG - Chr7:73349786-73349805 None:intergenic 75.0%
! GCGGACGAGGACCTCGCGGC+GGG - Chr7:73349767-73349786 None:intergenic 80.0%
! GGCGGACGAGGACCTCGCGG+CGG - Chr7:73349768-73349787 None:intergenic 80.0%
! GGCGGCGGACGAGGACCTCG+CGG - Chr7:73349771-73349790 None:intergenic 80.0%
! CACGGCGGAGGCGGCGGACG+AGG - Chr7:73349780-73349799 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
Chr7 gene 73349631 73351964 73349631 ID=MsG0780040127.01;Name=MsG0780040127.01
Chr7 mRNA 73349631 73351964 73349631 ID=MsG0780040127.01.T01;Parent=MsG0780040127.01;Name=MsG0780040127.01.T01;_AED=0.49;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|235
Chr7 exon 73349631 73350088 73349631 ID=MsG0780040127.01.T01:exon:13242;Parent=MsG0780040127.01.T01
Chr7 exon 73351715 73351964 73351715 ID=MsG0780040127.01.T01:exon:13243;Parent=MsG0780040127.01.T01
Chr7 CDS 73349631 73350088 73349631 ID=MsG0780040127.01.T01:cds;Parent=MsG0780040127.01.T01
Chr7 CDS 73351715 73351964 73351715 ID=MsG0780040127.01.T01:cds;Parent=MsG0780040127.01.T01
Gene Sequence

>MsG0780040127.01.T01

ACGCCGAAAATCTCTCCAATAAATCCACCACCACCAATGGAAGACTCTCAACAGCAACCACCGCAACAAATCGCGCCGATGACAATCGACGGTGCCGACGAAGACTTAGCCCTCGCTAACTCCTCCGTTTTAACCCGCCGCGAGGTCCTCGTCCGCCGCCTCCGCCGTGTCAGACAACTCTCTCGCTGTTACCGAGGTCATTACTGGGCGTTAATGGAAGAGCTTAAGGCTAAGTACAGAGAGTATTACTGGACTTATGGAAGGAGTCCGTTCAAGGAAGATGAAGAAAGAAATTCGGGTAACGCTTCTGCTGCTGTTGATAATGGTGATGACTTTGTTCGGTGTGCTTCTAGTGGTTGCAAGACCAAGGCTATGGCTCTTACTAAGTATTGTCATGCGCATATTTTGTCTGATCCTAATCAGAAGCTTTATCGTGGTTGCACCGCCGTGGCAAAAAGTTTGCCAACAGGGCCAGTCTTTTGTAATAAGCCAGTATTGAGATCCATGGTTCCTTTGGTATGCCCTGCTCATTACCAATTAGGCGAAAAATGCTTGCTTCGTGCTGTAAAAAGGGCAGGTCATAACGTCCCAGTTAATGGTAAGCCTAGTCCGCAGTTACATCTATTAATTCCTGAATTTGTCCGCCAAATTCAAAATAAACGAAAAGCTGCGCGCAAGGCAGCTTTACCTAAACCTGAGACTGATTAA
Protein sequence

>MsG0780040127.01.T01

TPKISPINPPPPMEDSQQQPPQQIAPMTIDGADEDLALANSSVLTRREVLVRRLRRVRQLSRCYRGHYWALMEELKAKYREYYWTYGRSPFKEDEERNSGNASAAVDNGDDFVRCASSGCKTKAMALTKYCHAHILSDPNQKLYRGCTAVAKSLPTGPVFCNKPVLRSMVPLVCPAHYQLGEKCLLRAVKRAGHNVPVNGKPSPQLHLLIPEFVRQIQNKRKAARKAALPKPETD*