AlfalfaGEDB | MsG0280007711.01

M. sativa cultivar ZhongmuNo.1 / MsG0280007711.01

KEGG: K23146
GO: GO:0004616 GO:0016491 GO:0055114
PFAM: PF03446

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007711.01.T01	KEH37064.1	77.778	81	7	1	1	81	1	70	2.19E-26	108

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007711.01.T01	A0A072V4W6	77.778	81	7	1	1	81	1	70	1.05e-26	108

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180003669.01	MsG0280007711.01	0.802136	6.472065e-49	3.146033e-46
MsG0180004149.01	MsG0280007711.01	0.801257	9.808135e-49	4.662682e-46
MsG0180004996.01	MsG0280007711.01	0.811064	8.440779e-51	5.173643e-48
MsG0280007711.01	MsG0280008204.01	0.800804	1.213371e-48	5.702477e-46
MsG0280007711.01	MsG0280009813.01	0.801395	9.186419e-49	4.382291e-46
MsG0280007711.01	MsG0280011093.01	0.840433	8.721989e-58	1.242152e-54
MsG0280007711.01	MsG0380014573.01	0.813811	2.118274e-51	1.397434e-48
MsG0280007711.01	MsG0380014647.01	0.802614	5.159602e-49	2.538820e-46
MsG0280007711.01	MsG0380016131.01	0.804553	2.043865e-49	1.056936e-46
MsG0280007711.01	MsG0380016355.01	0.805983	1.025515e-49	5.502287e-47
MsG0280007711.01	MsG0480020090.01	0.807112	5.925424e-50	3.272395e-47
MsG0280007711.01	MsG0480023535.01	0.805471	1.314049e-49	6.957797e-47
MsG0280007711.01	MsG0580024038.01	0.803968	2.706176e-49	1.378593e-46
MsG0280007711.01	MsG0580024981.01	0.831619	1.506668e-55	1.643844e-52
MsG0280007711.01	MsG0580025758.01	0.811862	5.662632e-51	3.544633e-48
MsG0280007711.01	MsG0580026002.01	0.819633	1.048143e-52	8.109727e-50
MsG0280007711.01	MsG0580026047.01	0.809777	1.600207e-50	9.480776e-48
MsG0280007711.01	MsG0580027031.01	0.800887	1.167437e-48	5.498442e-46
MsG0280007711.01	MsG0680033792.01	0.801764	7.718968e-49	3.716686e-46
MsG0280007711.01	MsG0680035346.01	0.815251	1.017163e-51	6.976558e-49
MsG0280007711.01	MsG0680035475.01	0.842581	2.369609e-58	3.609043e-55
MsG0280007711.01	MsG0680035876.01	0.801529	8.624165e-49	4.128090e-46
MsG0280007711.01	MsG0780037942.01	0.806033	1.001342e-49	5.379196e-47
MsG0280007711.01	MsG0780040162.01	0.818581	1.819024e-52	1.366964e-49
MsG0280007711.01	MsG0780041559.01	0.800278	1.553567e-48	7.204665e-46
MsG0280007711.01	MsG0880042779.01	0.809452	1.878901e-50	1.103555e-47
MsG0280007711.01	MsG0880043144.01	0.851177	1.051605e-60	2.110610e-57
MsG0280007711.01	MsG0880044415.01	0.820854	5.503653e-53	4.405615e-50
MsG0280007711.01	MsG0880044782.01	0.800455	1.429829e-48	6.660404e-46
MsG0280007711.01	MsG0880045042.01	0.800514	1.390707e-48	6.487986e-46
MsG0280007711.01	MsG0880045118.01	0.844925	5.589908e-59	9.164155e-56
MsG0280007711.01	MsG0880045691.01	0.801350	9.385465e-49	4.472301e-46
MsG0280007711.01	MsG0880047191.01	0.806622	7.520883e-50	4.101166e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007711.01.T01	MTR_2g030400	77.778	81	7	1	1	81	1	70	2.65e-30	108
MsG0280007711.01.T01	MTR_2g030400	77.778	81	7	1	1	81	1	70	3.46e-30	108
MsG0280007711.01.T01	MTR_2g030400	77.778	81	7	1	1	81	1	70	5.08e-30	108
MsG0280007711.01.T01	MTR_2g030400	77.778	81	7	1	1	81	1	70	1.17e-29	107

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 16 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCAATCAATCATCACTTGC+AGG	0.277191	2:-19525610	MsG0280007711.01.T01:intron
ATACAGAATGTTGGATTCAT+TGG	0.333722	2:-19525209	MsG0280007711.01.T01:CDS
TGATTAAATTATTCGCCATT+CGG	0.370921	2:+19525168	None:intergenic
GATTAAATTATTCGCCATTC+GGG	0.386047	2:+19525169	None:intergenic
AGATAGCTATGAAGATTTGC+AGG	0.401997	2:-19525716	MsG0280007712.01.T01:exon
AATGTTGGATTCATTGGCCT+TGG	0.415991	2:-19525203	MsG0280007711.01.T01:CDS
GATGGTTTGAGAATTACATT+TGG	0.426174	2:+19525668	None:intergenic
ATGGTTTGAGAATTACATTT+GGG	0.441341	2:+19525669	None:intergenic
GCGAATAATTTAATCAAAGC+TGG	0.441589	2:-19525161	MsG0280007711.01.T01:CDS
AAACTATGTGCTGCTGCAAC+TGG	0.466893	2:+19525636	None:intergenic
ATTCATTGGCCTTGGAAACA+TGG	0.478471	2:-19525195	MsG0280007711.01.T01:CDS
TGCAACTGGTGATGTTGAGA+TGG	0.522019	2:+19525650	None:intergenic
TTCATTGGCCTTGGAAACAT+GGG	0.542415	2:-19525194	MsG0280007711.01.T01:CDS
TTCGGGATCCCATGTTTCCA+AGG	0.543679	2:+19525186	None:intergenic
ATGCACTACATACAGAATGT+TGG	0.559585	2:-19525218	MsG0280007711.01.T01:CDS
TGGAAACATGGGATCCCGAA+TGG	0.593172	2:-19525183	MsG0280007711.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GTTAATTAGATCATATTAAT+TGG	-	Chr2:19525394-19525413	MsG0280007711.01.T01:intron	15.0%
!!	TATAAAAAAAATGCATGTTA+AGG	+	Chr2:19525283-19525302	None:intergenic	15.0%
!!	AGTAAAATTTCAGCTTATAA+AGG	+	Chr2:19525453-19525472	None:intergenic	20.0%
!!!	ATCATTAACTGTTTCTTTAT+AGG	-	Chr2:19525502-19525521	MsG0280007711.01.T01:intron	20.0%
!	ATGGTTTGAGAATTACATTT+GGG	+	Chr2:19525175-19525194	None:intergenic	25.0%
!	TGATTAAATTATTCGCCATT+CGG	+	Chr2:19525676-19525695	None:intergenic	25.0%
!	TTTCATGCATATTCTTAACT+GGG	-	Chr2:19525342-19525361	MsG0280007711.01.T01:intron	25.0%
	ATACAGAATGTTGGATTCAT+TGG	-	Chr2:19525632-19525651	MsG0280007711.01.T01:CDS	30.0%
	CAGTTAAGAATATGCATGAA+AGG	+	Chr2:19525343-19525362	None:intergenic	30.0%
	CTTTCATGCATATTCTTAAC+TGG	-	Chr2:19525341-19525360	MsG0280007711.01.T01:intron	30.0%
	GATGGTTTGAGAATTACATT+TGG	+	Chr2:19525176-19525195	None:intergenic	30.0%
	GATTAAATTATTCGCCATTC+GGG	+	Chr2:19525675-19525694	None:intergenic	30.0%
	GCGAATAATTTAATCAAAGC+TGG	-	Chr2:19525680-19525699	MsG0280007711.01.T01:CDS	30.0%
	TAGCATACAAAAATTGTAGC+TGG	+	Chr2:19525529-19525548	None:intergenic	30.0%
	ATGCACTACATACAGAATGT+TGG	-	Chr2:19525623-19525642	MsG0280007711.01.T01:CDS	35.0%
	TAAAGGCAAAACCAATCACA+TGG	+	Chr2:19525436-19525455	None:intergenic	35.0%
	TGTGTTTATCTCCATGTGAT+TGG	-	Chr2:19525422-19525441	MsG0280007711.01.T01:intron	35.0%
	TTCAATCAATCATCACTTGC+AGG	-	Chr2:19525231-19525250	MsG0280007711.01.T01:CDS	35.0%
!	AATGTTGGATTCATTGGCCT+TGG	-	Chr2:19525638-19525657	MsG0280007711.01.T01:CDS	40.0%
!!	ATTCATTGGCCTTGGAAACA+TGG	-	Chr2:19525646-19525665	MsG0280007711.01.T01:CDS	40.0%
!!	TTCATTGGCCTTGGAAACAT+GGG	-	Chr2:19525647-19525666	MsG0280007711.01.T01:CDS	40.0%
!	AAACTATGTGCTGCTGCAAC+TGG	+	Chr2:19525208-19525227	None:intergenic	45.0%
!	TGCAACTGGTGATGTTGAGA+TGG	+	Chr2:19525194-19525213	None:intergenic	45.0%
	TGGAAACATGGGATCCCGAA+TGG	-	Chr2:19525658-19525677	MsG0280007711.01.T01:CDS	50.0%
	TTCGGGATCCCATGTTTCCA+AGG	+	Chr2:19525658-19525677	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	19525133	19525730	19525133	ID=MsG0280007711.01;Name=MsG0280007711.01
Chr2	mRNA	19525133	19525730	19525133	ID=MsG0280007711.01.T01;Parent=MsG0280007711.01;Name=MsG0280007711.01.T01;_AED=0.48;_eAED=0.51;_QI=0\|0\|0\|1\|0\|0\|2\|0\|81
Chr2	exon	19525611	19525730	19525611	ID=MsG0280007711.01.T01:exon:2275;Parent=MsG0280007711.01.T01
Chr2	exon	19525133	19525258	19525133	ID=MsG0280007711.01.T01:exon:2274;Parent=MsG0280007711.01.T01
Chr2	CDS	19525611	19525730	19525611	ID=MsG0280007711.01.T01:cds;Parent=MsG0280007711.01.T01
Chr2	CDS	19525133	19525258	19525133	ID=MsG0280007711.01.T01:cds;Parent=MsG0280007711.01.T01

Gene Sequence	>MsG0280007711.01.T01 ATGAAGATTTGCAGGTTGACATCTCTGTTTTCTTCTCTTCCCAAATGTAATTCTCAAACCATCTCAACATCACCAGTTGCAGCAGCACATAGTTTCTCTTCAATCAATCATCACTTGCAGCTGATGATACTGTCTATGATGCACTACATACAGAATGTTGGATTCATTGGCCTTGGAAACATGGGATCCCGAATGGCGAATAATTTAATCAAAGCTGGCTTCAAACTTACAGTTCATGACAAGTAA
Protein sequence	>MsG0280007711.01.T01 MKICRLTSLFSSLPKCNSQTISTSPVAAAHSFSSINHHLQLMILSMMHYIQNVGFIGLGNMGSRMANNLIKAGFKLTVHDK*