AlfalfaGEDB | MsG0280009813.01

M. sativa cultivar ZhongmuNo.1 / MsG0280009813.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009813.01.T02	XP_003596225.2	94.444	72	4	0	1	72	84	155	1.15E-40	140
MsG0280009813.01.T01	XP_003596225.2	93.548	155	10	0	1	155	1	155	6.78E-101	296

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009813.01.T01	G7IJC5	93.548	155	10	0	1	155	1	155	3.24e-101	296
MsG0280009813.01.T02	G7IJC5	94.444	72	4	0	1	72	84	155	5.48e-41	140

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180001687.01	MsG0280009813.01	0.838839	2.265651e-57	3.071872e-54
MsG0180002071.01	MsG0280009813.01	0.815501	8.950943e-52	6.182239e-49
MsG0180003586.01	MsG0280009813.01	0.808339	3.250924e-50	1.854056e-47
MsG0180003669.01	MsG0280009813.01	0.820806	5.647042e-53	4.514214e-50
MsG0180004149.01	MsG0280009813.01	0.809818	1.567650e-50	9.297588e-48
MsG0180004260.01	MsG0280009813.01	0.823265	1.520027e-53	1.302393e-50
MsG0180004996.01	MsG0280009813.01	0.821044	4.977259e-53	4.005613e-50
MsG0180005371.01	MsG0280009813.01	0.850198	1.982526e-60	3.852945e-57
MsG0280007609.01	MsG0280009813.01	0.802972	4.353151e-49	2.161483e-46
MsG0280007711.01	MsG0280009813.01	0.801395	9.186419e-49	4.382291e-46
MsG0280008615.01	MsG0280009813.01	0.838725	2.425012e-57	3.276766e-54
MsG0280009514.01	MsG0280009813.01	0.811981	5.334291e-51	3.349861e-48
MsG0280009748.01	MsG0280009813.01	0.805167	1.521234e-49	7.992376e-47
MsG0280009813.01	MsG0280010389.01	0.842841	2.020523e-58	3.102269e-55
MsG0280009813.01	MsG0280010551.01	0.802899	4.506905e-49	2.233837e-46
MsG0280009813.01	MsG0280010614.01	0.804340	2.264584e-49	1.164676e-46
MsG0280009813.01	MsG0280011070.01	0.815378	9.534367e-52	6.562556e-49
MsG0280009813.01	MsG0280011388.01	0.802593	5.212816e-49	2.563635e-46
MsG0280009813.01	MsG0380014573.01	0.824428	8.115043e-54	7.185504e-51
MsG0280009813.01	MsG0380015677.01	0.810757	9.836723e-51	5.980355e-48
MsG0280009813.01	MsG0380017266.01	-0.818016	2.442069e-52	1.806853e-49
MsG0280009813.01	MsG0380017323.01	0.803621	3.194843e-49	1.613045e-46
MsG0280009813.01	MsG0480018318.01	0.806133	9.537194e-50	5.136489e-47
MsG0280009813.01	MsG0480020090.01	0.805472	1.313228e-49	6.953667e-47
MsG0280009813.01	MsG0480020445.01	0.808782	2.614500e-50	1.508352e-47
MsG0280009813.01	MsG0480022827.01	0.816548	5.225454e-52	3.713639e-49
MsG0280009813.01	MsG0480023654.01	0.821272	4.410819e-53	3.571726e-50
MsG0280009813.01	MsG0480023718.01	0.831594	1.527558e-55	1.665503e-52
MsG0280009813.01	MsG0580024418.01	0.801339	9.432900e-49	4.493616e-46
MsG0280009813.01	MsG0580025555.01	0.806350	8.586011e-50	4.649704e-47
MsG0280009813.01	MsG0580025758.01	0.829776	4.260429e-55	4.404228e-52
MsG0280009813.01	MsG0580027320.01	0.801804	7.573670e-49	3.650571e-46
MsG0280009813.01	MsG0580027344.01	0.807035	6.152475e-50	3.391140e-47
MsG0280009813.01	MsG0580027628.01	0.814181	1.755943e-51	1.170028e-48
MsG0280009813.01	MsG0580028517.01	0.808675	2.755774e-50	1.585424e-47
MsG0280009813.01	MsG0580028592.01	0.804153	2.475973e-49	1.267340e-46
MsG0280009813.01	MsG0580028991.01	0.809157	2.174127e-50	1.266976e-47
MsG0280009813.01	MsG0580029950.01	0.815541	8.767940e-52	6.062346e-49
MsG0280009813.01	MsG0680030316.01	0.851882	6.643765e-61	1.363753e-57
MsG0280009813.01	MsG0680030447.01	0.802939	4.421994e-49	2.193860e-46
MsG0280009813.01	MsG0680030656.01	0.828208	1.021256e-54	1.008366e-51
MsG0280009813.01	MsG0680030672.01	0.825956	3.532581e-54	3.267880e-51
MsG0280009813.01	MsG0680031114.01	0.825810	3.826713e-54	3.525323e-51
MsG0280009813.01	MsG0680031133.01	0.814662	1.374280e-51	9.277093e-49
MsG0280009813.01	MsG0680031549.01	0.817883	2.617698e-52	1.929715e-49
MsG0280009813.01	MsG0680032203.01	0.808108	3.642397e-50	2.064557e-47
MsG0280009813.01	MsG0680033228.01	0.800189	1.619474e-48	7.493110e-46
MsG0280009813.01	MsG0680033792.01	0.825541	4.430459e-54	4.050070e-51
MsG0280009813.01	MsG0680035389.01	0.808679	2.751070e-50	1.582870e-47
MsG0280009813.01	MsG0680035475.01	0.832997	6.865707e-56	7.803834e-53
MsG0280009813.01	MsG0780038386.01	0.816019	6.862414e-52	4.807294e-49
MsG0280009813.01	MsG0780038391.01	0.839105	1.933709e-57	2.643133e-54
MsG0280009813.01	MsG0780038937.01	0.804350	2.253665e-49	1.159365e-46
MsG0280009813.01	MsG0780039452.01	0.815930	7.183579e-52	5.019725e-49
MsG0280009813.01	MsG0780040947.01	0.805356	1.388669e-49	7.331509e-47
MsG0280009813.01	MsG0880041986.01	0.814491	1.499954e-51	1.007934e-48
MsG0280009813.01	MsG0880043144.01	0.822823	1.927277e-53	1.630959e-50
MsG0280009813.01	MsG0880043992.01	0.811140	8.125298e-51	4.990248e-48
MsG0280009813.01	MsG0880044075.01	0.817919	2.568445e-52	1.895351e-49
MsG0280009813.01	MsG0880044237.01	0.804425	2.173184e-49	1.120095e-46
MsG0280009813.01	MsG0880044477.01	0.813438	2.559008e-51	1.671203e-48
MsG0280009813.01	MsG0880044509.01	0.838831	2.276476e-57	3.085755e-54
MsG0280009813.01	MsG0880044510.01	0.824850	6.455432e-54	5.786523e-51
MsG0280009813.01	MsG0880044542.01	0.850948	1.220323e-60	2.430897e-57
MsG0280009813.01	MsG0880044554.01	0.832264	1.043899e-55	1.160616e-52
MsG0280009813.01	MsG0880044573.01	0.802471	5.522786e-49	2.707611e-46
MsG0280009813.01	MsG0880044782.01	0.812970	3.242683e-51	2.090785e-48
MsG0280009813.01	MsG0880045118.01	0.814488	1.501594e-51	1.008975e-48
MsG0280009813.01	MsG0880046669.01	0.820869	5.461164e-53	4.373416e-50
MsG0280009813.01	MsG0880046762.01	0.813112	3.018572e-51	1.953658e-48
MsG0280009813.01	MsG0880047191.01	0.860127	2.565333e-63	6.938716e-60

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009813.01.T02	MTR_2g071790	94.444	72	4	0	1	72	84	155	1.39e-44	140
MsG0280009813.01.T02	MTR_2g071760	84.722	72	11	0	1	72	84	155	7.33e-40	128
MsG0280009813.01.T02	MTR_2g071750	81.944	72	13	0	1	72	84	155	2.56e-38	124
MsG0280009813.01.T02	MTR_2g071740	77.143	70	10	2	1	69	84	148	1.40e-29	102
MsG0280009813.01.T01	MTR_2g071790	93.548	155	10	0	1	155	1	155	8.21e-105	296
MsG0280009813.01.T01	MTR_2g071760	88.387	155	18	0	1	155	1	155	2.13e-96	275
MsG0280009813.01.T01	MTR_2g071750	86.452	155	21	0	1	155	1	155	4.03e-96	274
MsG0280009813.01.T01	MTR_2g071740	85.621	153	16	2	1	152	1	148	2.40e-89	257

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 40 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGGGTTGAGTGGGTGGATA+TGG	0.201977	2:-58579842	MsG0280009813.01.T01:CDS
TGTGATGGTTGATCATTTAA+TGG	0.277636	2:-58579749	MsG0280009813.01.T01:CDS
CCCAACTTGCATCATCAAAT+TGG	0.284176	2:+58579803	None:intergenic
TTGTGATGATGGGTTTGATA+TGG	0.302331	2:-58579725	MsG0280009813.01.T01:CDS
TGGAGGAGGGACATGACTTT+GGG	0.323166	2:-58579861	MsG0280009813.01.T01:CDS
AACATGCTTACAATTCCTTT+TGG	0.331685	2:-58579699	MsG0280009813.01.T01:CDS
CATTTAATGGTTTGTGATGA+TGG	0.359988	2:-58579736	MsG0280009813.01.T01:CDS
CCAATTTGATGATGCAAGTT+GGG	0.373282	2:-58579803	MsG0280009813.01.T01:CDS
AAGAATATTATGATGAATCA+TGG	0.395210	2:-58579900	MsG0280009813.01.T01:CDS
ATGGAGGAGGGACATGACTT+TGG	0.417476	2:-58579862	MsG0280009813.01.T01:CDS
TGCTTGTGATGTGGAAATCA+AGG	0.457434	2:-58580085	MsG0280009813.01.T01:CDS
GACATGACTTTGGGGTTGAG+TGG	0.467986	2:-58579852	MsG0280009813.01.T01:CDS
TCCAATTTGATGATGCAAGT+TGG	0.473251	2:-58579804	MsG0280009813.01.T01:CDS
CTATGCATGTTGAATTATCA+TGG	0.491083	2:+58579947	None:intergenic
ATTTAATGGTTTGTGATGAT+GGG	0.498358	2:-58579735	MsG0280009813.01.T01:CDS
CTCATACCTATCTTGCCAAA+AGG	0.500774	2:+58579684	None:intergenic
ACATGACTTTGGGGTTGAGT+GGG	0.501007	2:-58579851	MsG0280009813.01.T01:CDS
TGAAATTAGTGAAAGCAAGA+TGG	0.502049	2:-58579974	MsG0280009813.01.T01:CDS
TGATCTTCTCACGTCACTGA+TGG	0.502867	2:-58580061	MsG0280009813.01.T01:CDS
TGGCAAGATAGGTATGAGAT+GGG	0.517786	2:-58579679	MsG0280009813.01.T01:CDS
TTGGCAAGATAGGTATGAGA+TGG	0.518652	2:-58579680	MsG0280009813.01.T01:CDS
TGACTTTGGGGTTGAGTGGG+TGG	0.527133	2:-58579848	MsG0280009813.01.T01:CDS
TATGATGAATCATGGAACAT+AGG	0.530061	2:-58579892	MsG0280009813.01.T01:CDS
CATGATAATTCAACATGCAT+AGG	0.547359	2:-58579946	MsG0280009813.01.T01:CDS
GAAATTAGTGAAAGCAAGAT+GGG	0.562708	2:-58579973	MsG0280009813.01.T01:CDS
ATGGAACATAGGACAAGTGA+TGG	0.569406	2:-58579881	MsG0280009813.01.T01:CDS
GGAGGAGGGACATGACTTTG+GGG	0.584089	2:-58579860	MsG0280009813.01.T01:CDS
TGGGAGGCAATTGAGTCTTG+TGG	0.585282	2:-58579784	MsG0280009813.01.T01:CDS
CATAGGACAAGTGATGGAGG+AGG	0.595117	2:-58579875	MsG0280009813.01.T01:CDS
ACTTGCATCATCAAATTGGA+AGG	0.605601	2:+58579807	None:intergenic
TGGAGATGAGAAAGATGTGA+TGG	0.610494	2:-58579764	MsG0280009813.01.T01:CDS
GGCAAGATAGGTATGAGATG+GGG	0.613154	2:-58579678	MsG0280009813.01.T01:CDS
GCTTGTGATGTGGAAATCAA+GGG	0.616324	2:-58580084	MsG0280009813.01.T01:CDS
AGATAGGTATGAGATGGGGT+TGG	0.636490	2:-58579674	MsG0280009813.01.T01:CDS
ATTTGATGATGCAAGTTGGG+AGG	0.639497	2:-58579800	MsG0280009813.01.T01:CDS
GAACATAGGACAAGTGATGG+AGG	0.641704	2:-58579878	MsG0280009813.01.T01:CDS
TCATCATTACTCTCATCACA+TGG	0.648258	2:+58580030	None:intergenic
GTGATGAGAGTAATGATGAA+AGG	0.650915	2:-58580026	MsG0280009813.01.T01:CDS
ATAGGACAAGTGATGGAGGA+GGG	0.671083	2:-58579874	MsG0280009813.01.T01:CDS
ATTAGTGAAAGCAAGATGGG+TGG	0.695184	2:-58579970	MsG0280009813.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGAATATTATGATGAATCA+TGG	-	Chr2:58579877-58579896	MsG0280009813.01.T01:CDS	20.0%
!!!	TAGTTTAATAAGATCTTTTG+AGG	-	Chr2:58579779-58579798	MsG0280009813.01.T01:CDS	20.0%
!!	ATTTAATGGTTTGTGATGAT+GGG	-	Chr2:58580042-58580061	MsG0280009813.01.T01:CDS	25.0%
	CATGATAATTCAACATGCAT+AGG	-	Chr2:58579831-58579850	MsG0280009813.01.T01:CDS	30.0%
	CTATGCATGTTGAATTATCA+TGG	+	Chr2:58579833-58579852	None:intergenic	30.0%
	GAAATTAGTGAAAGCAAGAT+GGG	-	Chr2:58579804-58579823	MsG0280009813.01.T01:CDS	30.0%
	TATGATGAATCATGGAACAT+AGG	-	Chr2:58579885-58579904	MsG0280009813.01.T01:CDS	30.0%
	TGAAATTAGTGAAAGCAAGA+TGG	-	Chr2:58579803-58579822	MsG0280009813.01.T01:CDS	30.0%
	TGTGATGGTTGATCATTTAA+TGG	-	Chr2:58580028-58580047	MsG0280009813.01.T01:CDS	30.0%
!	AACATGCTTACAATTCCTTT+TGG	-	Chr2:58580078-58580097	MsG0280009813.01.T01:CDS	30.0%
!	CATTTAATGGTTTGTGATGA+TGG	-	Chr2:58580041-58580060	MsG0280009813.01.T01:CDS	30.0%
	ACTTGCATCATCAAATTGGA+AGG	+	Chr2:58579973-58579992	None:intergenic	35.0%
	GTGATGAGAGTAATGATGAA+AGG	-	Chr2:58579751-58579770	MsG0280009813.01.T01:CDS	35.0%
	TCATCATTACTCTCATCACA+TGG	+	Chr2:58579750-58579769	None:intergenic	35.0%
!	ACAATTCCTTTTGGCAAGAT+AGG	-	Chr2:58580087-58580106	MsG0280009813.01.T01:CDS	35.0%
!	TGATACTTTTGCTTGTGATG+TGG	-	Chr2:58579683-58579702	MsG0280009813.01.T01:CDS	35.0%
!!	CCAATTTGATGATGCAAGTT+GGG	-	Chr2:58579974-58579993	MsG0280009813.01.T01:CDS	35.0%
!!	TCCAATTTGATGATGCAAGT+TGG	-	Chr2:58579973-58579992	MsG0280009813.01.T01:CDS	35.0%
!!	TTGTGATGATGGGTTTGATA+TGG	-	Chr2:58580052-58580071	MsG0280009813.01.T01:CDS	35.0%
	ATGGAACATAGGACAAGTGA+TGG	-	Chr2:58579896-58579915	MsG0280009813.01.T01:CDS	40.0%
	ATTAGTGAAAGCAAGATGGG+TGG	-	Chr2:58579807-58579826	MsG0280009813.01.T01:CDS	40.0%
	CCCAACTTGCATCATCAAAT+TGG	+	Chr2:58579977-58579996	None:intergenic	40.0%
	CTCATACCTATCTTGCCAAA+AGG	+	Chr2:58580096-58580115	None:intergenic	40.0%
	GCTTGTGATGTGGAAATCAA+GGG	-	Chr2:58579693-58579712	MsG0280009813.01.T01:CDS	40.0%
	TGCTTGTGATGTGGAAATCA+AGG	-	Chr2:58579692-58579711	MsG0280009813.01.T01:CDS	40.0%
	TGGAGATGAGAAAGATGTGA+TGG	-	Chr2:58580013-58580032	MsG0280009813.01.T01:CDS	40.0%
	TGGCAAGATAGGTATGAGAT+GGG	-	Chr2:58580098-58580117	MsG0280009813.01.T01:CDS	40.0%
	TTGGCAAGATAGGTATGAGA+TGG	-	Chr2:58580097-58580116	MsG0280009813.01.T01:CDS	40.0%
!!	ATTTGATGATGCAAGTTGGG+AGG	-	Chr2:58579977-58579996	MsG0280009813.01.T01:CDS	40.0%
	AGATAGGTATGAGATGGGGT+TGG	-	Chr2:58580103-58580122	MsG0280009813.01.T01:CDS	45.0%
	GAACATAGGACAAGTGATGG+AGG	-	Chr2:58579899-58579918	MsG0280009813.01.T01:CDS	45.0%
	GGCAAGATAGGTATGAGATG+GGG	-	Chr2:58580099-58580118	MsG0280009813.01.T01:CDS	45.0%
	TGATCTTCTCACGTCACTGA+TGG	-	Chr2:58579716-58579735	MsG0280009813.01.T01:CDS	45.0%
!	ATAGGACAAGTGATGGAGGA+GGG	-	Chr2:58579903-58579922	MsG0280009813.01.T01:CDS	45.0%
!!	ACATGACTTTGGGGTTGAGT+GGG	-	Chr2:58579926-58579945	MsG0280009813.01.T01:CDS	45.0%
	ATGGAGGAGGGACATGACTT+TGG	-	Chr2:58579915-58579934	MsG0280009813.01.T01:CDS	50.0%
	TGGAGGAGGGACATGACTTT+GGG	-	Chr2:58579916-58579935	MsG0280009813.01.T01:CDS	50.0%
	TGGGAGGCAATTGAGTCTTG+TGG	-	Chr2:58579993-58580012	MsG0280009813.01.T01:CDS	50.0%
!	CATAGGACAAGTGATGGAGG+AGG	-	Chr2:58579902-58579921	MsG0280009813.01.T01:CDS	50.0%
!!	GACATGACTTTGGGGTTGAG+TGG	-	Chr2:58579925-58579944	MsG0280009813.01.T01:CDS	50.0%
	GGAGGAGGGACATGACTTTG+GGG	-	Chr2:58579917-58579936	MsG0280009813.01.T01:CDS	55.0%
	TGGGGTTGAGTGGGTGGATA+TGG	-	Chr2:58579935-58579954	MsG0280009813.01.T01:CDS	55.0%
!!	TGACTTTGGGGTTGAGTGGG+TGG	-	Chr2:58579929-58579948	MsG0280009813.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	58579666	58580133	58579666	ID=MsG0280009813.01;Name=MsG0280009813.01
Chr2	mRNA	58579666	58580133	58579666	ID=MsG0280009813.01.T01;Parent=MsG0280009813.01;Name=MsG0280009813.01.T01;_AED=0.29;_eAED=0.29;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|155
Chr2	exon	58579666	58580133	58579666	ID=MsG0280009813.01.T01:exon:65575;Parent=MsG0280009813.01.T01
Chr2	CDS	58579666	58580133	58579666	ID=MsG0280009813.01.T01:cds;Parent=MsG0280009813.01.T01
Chr2	mRNA	58579666	58579884	58579666	ID=MsG0280009813.01.T02;Parent=MsG0280009813.01;Name=MsG0280009813.01.T02;_AED=0.40;_eAED=0.40;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|72
Chr2	exon	58579666	58579884	58579666	ID=MsG0280009813.01.T02:exon:65576;Parent=MsG0280009813.01.T02
Chr2	CDS	58579666	58579884	58579666	ID=MsG0280009813.01.T02:cds;Parent=MsG0280009813.01.T02

Gene Sequence

>MsG0280009813.01.T02

ATGGAGGAGGGACATGACTTTGGGGTTGAGTGGGTGGATATGGATTTGATGACATCCTTCCAATTTGATGATGCAAGTTGGGAGGCAATTGAGTCTTGTGGAGATGAGAAAGATGTGATGGTTGATCATTTAATGGTTTGTGATGATGGGTTTGATATGGAAGAACATGCTTACAATTCCTTTTGGCAAGATAGGTATGAGATGGGGTTGGTGCATTAG

>MsG0280009813.01.T01

ATGGCTTCTCAAGTTTGTGATACTTTTGCTTGTGATGTGGAAATCAAGGGTGATCTTCTCACGTCACTGATGGAAGAGTTACCATGTGATGAGAGTAATGATGAAAGGTTAGATAGTTTAATAAGATCTTTTGAGGCTGAAATTAGTGAAAGCAAGATGGGTGGCCATGATAATTCAACATGCATAGGATCACAATTGAAGAGCAATTTTGAAGAATATTATGATGAATCATGGAACATAGGACAAGTGATGGAGGAGGGACATGACTTTGGGGTTGAGTGGGTGGATATGGATTTGATGACATCCTTCCAATTTGATGATGCAAGTTGGGAGGCAATTGAGTCTTGTGGAGATGAGAAAGATGTGATGGTTGATCATTTAATGGTTTGTGATGATGGGTTTGATATGGAAGAACATGCTTACAATTCCTTTTGGCAAGATAGGTATGAGATGGGGTTGGTGCATTAG

Protein sequence

>MsG0280009813.01.T02

MEEGHDFGVEWVDMDLMTSFQFDDASWEAIESCGDEKDVMVDHLMVCDDGFDMEEHAYNSFWQDRYEMGLVH*

>MsG0280009813.01.T01

MASQVCDTFACDVEIKGDLLTSLMEELPCDESNDERLDSLIRSFEAEISESKMGGHDNSTCIGSQLKSNFEEYYDESWNIGQVMEEGHDFGVEWVDMDLMTSFQFDDASWEAIESCGDEKDVMVDHLMVCDDGFDMEEHAYNSFWQDRYEMGLVH*