AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035876.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035876.01.T01 MTR_6g093220 87.097 155 12 4 1 148 1 154 4.76e-73 219
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035876.01.T01 AT5G58110 58.824 136 53 2 14 148 3 136 2.71e-49 158

Find 41 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCGTCGTCGTATACTTAT+TGG 0.221609 6:+113801407 MsG0680035876.01.T01:CDS
TGTTACTTCTTCTCATCAAT+TGG 0.222046 6:+113801498 MsG0680035876.01.T01:CDS
TTGGGCTATTCCTAGAATTA+AGG 0.262161 6:+113801570 MsG0680035876.01.T01:CDS
ATGACGTCACTCCTCCATTA+AGG 0.273220 6:-113801354 None:intergenic
AGTAAGTAAACCTTAATTCT+AGG 0.282854 6:-113801580 None:intergenic
GAATCAATGGAGTCCCTTAA+TGG 0.345099 6:+113801340 MsG0680035876.01.T01:exon
GTTACTTCTTCTCATCAATT+GGG 0.369463 6:+113801499 MsG0680035876.01.T01:CDS
CTCATCAATTGGGTTCTGCT+TGG 0.379523 6:+113801509 MsG0680035876.01.T01:CDS
GGAGATCTCATTTGGTGAAC+TGG 0.386323 6:+113802224 MsG0680035876.01.T01:CDS
TGGTGAACTGGATGACTTGC+AGG 0.392831 6:+113802236 MsG0680035876.01.T01:CDS
ACTAACATCTCCAACACATT+TGG 0.402013 6:-113801755 None:intergenic
CTTGGAATCGTGCTGGCACT+TGG 0.404894 6:+113801527 MsG0680035876.01.T01:CDS
AGCGTCGTCGTATACTTATT+GGG 0.412195 6:+113801408 MsG0680035876.01.T01:CDS
GATGTTCCGGAGATCTCATT+TGG 0.428887 6:+113802216 MsG0680035876.01.T01:CDS
CAGGAATTGCTCATTTCTGT+GGG 0.441867 6:+113801682 MsG0680035876.01.T01:intron
TCTGCCAGAGGAGAATGATA+TGG 0.453679 6:-113801707 None:intergenic
TCAGGAATTGCTCATTTCTG+TGG 0.456847 6:+113801681 MsG0680035876.01.T01:intron
TGACGTCACTCCTCCATTAA+GGG 0.458538 6:-113801353 None:intergenic
CGTCGTATACTTATTGGGTT+CGG 0.478637 6:+113801413 MsG0680035876.01.T01:CDS
AAGAGAAATCTCTTAACCAT+TGG 0.487347 6:+113801551 MsG0680035876.01.T01:CDS
AGAGAAATCTCTTAACCATT+GGG 0.488159 6:+113801552 MsG0680035876.01.T01:CDS
GTGTTAAAATCAATAATAAG+AGG 0.491434 6:-113802261 None:intergenic
GGCGTTTGTTGAAGAATCAA+TGG 0.504390 6:+113801327 MsG0680035876.01.T01:exon
GGTTCCATATCATTCTCCTC+TGG 0.510440 6:+113801703 MsG0680035876.01.T01:CDS
TACAATTGGATACAGGGGAA+TGG 0.527980 6:+113802155 MsG0680035876.01.T01:intron
GGTTCTGCTTGGAATCGTGC+TGG 0.531758 6:+113801520 MsG0680035876.01.T01:CDS
TTATTTATTATAGGCACTTG+TGG 0.538772 6:+113801979 MsG0680035876.01.T01:intron
ATATCATTCTCCTCTGGCAG+AGG 0.546286 6:+113801709 MsG0680035876.01.T01:CDS
GAGCTGTCCTTAAAAGTGAA+AGG 0.567172 6:+113802040 MsG0680035876.01.T01:CDS
AGTTCACCAAATGAGATCTC+CGG 0.570544 6:-113802222 None:intergenic
CAAGATGTAACCAAATGTGT+TGG 0.572691 6:+113801745 MsG0680035876.01.T01:CDS
AAATAAATAAATCTTGTACT+CGG 0.587602 6:+113801242 MsG0680035876.01.T01:five_prime_UTR
GTCATCGTCGTCGAAATCGA+AGG 0.588472 6:+113801372 MsG0680035876.01.T01:CDS
TTGTGCTGTGCCTCTGCCAG+AGG 0.600109 6:-113801719 None:intergenic
TTGGAATCGTGCTGGCACTT+GGG 0.601928 6:+113801528 MsG0680035876.01.T01:CDS
TTTATTATAGGCACTTGTGG+TGG 0.610822 6:+113801982 MsG0680035876.01.T01:intron
ACAGGGGAATGGATTATACA+AGG 0.613504 6:+113802166 MsG0680035876.01.T01:intron
TTGTACTCGGATCGATCTAG+AGG 0.622502 6:+113801255 MsG0680035876.01.T01:five_prime_UTR
TAATTCTAGGAATAGCCCAA+TGG 0.650376 6:-113801567 None:intergenic
TCAATGGAGTCCCTTAATGG+AGG 0.676249 6:+113801343 MsG0680035876.01.T01:exon
TGAGTGAGTTGAAGTACGAG+CGG 0.714496 6:+113801306 MsG0680035876.01.T01:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATATTTATTTATTAT+AGG + Chr6:113801970-113801989 MsG0680035876.01.T01:intron 0.0%
!! AAATAAATAAATCTTGTACT+CGG + Chr6:113801242-113801261 MsG0680035876.01.T01:five_prime_UTR 15.0%
!! GTGTTAAAATCAATAATAAG+AGG - Chr6:113802264-113802283 None:intergenic 20.0%
!! TAGAATTTGAATTTGAAGAT+AGG - Chr6:113801804-113801823 None:intergenic 20.0%
!!! TATAAGTGATGTTTTACAAT+TGG + Chr6:113802141-113802160 MsG0680035876.01.T01:intron 20.0%
! AAGGAAATAAAAAGTTGCTT+GGG + Chr6:113802185-113802204 MsG0680035876.01.T01:CDS 25.0%
! AGTAAGTAAACCTTAATTCT+AGG - Chr6:113801583-113801602 None:intergenic 25.0%
! TTATTTATTATAGGCACTTG+TGG + Chr6:113801979-113801998 MsG0680035876.01.T01:intron 25.0%
!! ATGTTTTACAATTGGATACA+GGG + Chr6:113802149-113802168 MsG0680035876.01.T01:intron 25.0%
!!! TCATTTTGTTACTTTGTTTC+AGG + Chr6:113801663-113801682 MsG0680035876.01.T01:intron 25.0%
AAGAGAAATCTCTTAACCAT+TGG + Chr6:113801551-113801570 MsG0680035876.01.T01:CDS 30.0%
AGAGAAATCTCTTAACCATT+GGG + Chr6:113801552-113801571 MsG0680035876.01.T01:CDS 30.0%
AGGAAATAAAAAGTTGCTTG+GGG + Chr6:113802186-113802205 MsG0680035876.01.T01:CDS 30.0%
CAAGGAAATAAAAAGTTGCT+TGG + Chr6:113802184-113802203 MsG0680035876.01.T01:CDS 30.0%
GAATGAAAGAGTAATTGACT+CGG - Chr6:113801632-113801651 None:intergenic 30.0%
GCAAAGTAAAGAAGAACAAT+TGG - Chr6:113802103-113802122 None:intergenic 30.0%
GTTACTTCTTCTCATCAATT+GGG + Chr6:113801499-113801518 MsG0680035876.01.T01:CDS 30.0%
! GATGTTTTACAATTGGATAC+AGG + Chr6:113802148-113802167 MsG0680035876.01.T01:intron 30.0%
! TGTTACTTCTTCTCATCAAT+TGG + Chr6:113801498-113801517 MsG0680035876.01.T01:CDS 30.0%
! TGTTTTACAATTGGATACAG+GGG + Chr6:113802150-113802169 MsG0680035876.01.T01:intron 30.0%
ACTAACATCTCCAACACATT+TGG - Chr6:113801758-113801777 None:intergenic 35.0%
CAAGATGTAACCAAATGTGT+TGG + Chr6:113801745-113801764 MsG0680035876.01.T01:CDS 35.0%
GAAGAACAATTGGATTGAAG+TGG - Chr6:113802093-113802112 None:intergenic 35.0%
GGAAATAAAAAGTTGCTTGG+GGG + Chr6:113802187-113802206 MsG0680035876.01.T01:CDS 35.0%
TTGGGCTATTCCTAGAATTA+AGG + Chr6:113801570-113801589 MsG0680035876.01.T01:CDS 35.0%
TTTATTATAGGCACTTGTGG+TGG + Chr6:113801982-113802001 MsG0680035876.01.T01:intron 35.0%
! TAATTCTAGGAATAGCCCAA+TGG - Chr6:113801570-113801589 None:intergenic 35.0%
!! TGACTCACCTTTCACTTTTA+AGG - Chr6:113802050-113802069 None:intergenic 35.0%
AGCGTCGTCGTATACTTATT+GGG + Chr6:113801408-113801427 MsG0680035876.01.T01:CDS 40.0%
AGTTCACCAAATGAGATCTC+CGG - Chr6:113802225-113802244 None:intergenic 40.0%
CAGGAATTGCTCATTTCTGT+GGG + Chr6:113801682-113801701 MsG0680035876.01.T01:intron 40.0%
CGTCGTATACTTATTGGGTT+CGG + Chr6:113801413-113801432 MsG0680035876.01.T01:CDS 40.0%
GAATCAATGGAGTCCCTTAA+TGG + Chr6:113801340-113801359 MsG0680035876.01.T01:exon 40.0%
GAGCTGTCCTTAAAAGTGAA+AGG + Chr6:113802040-113802059 MsG0680035876.01.T01:CDS 40.0%
TACAATTGGATACAGGGGAA+TGG + Chr6:113802155-113802174 MsG0680035876.01.T01:intron 40.0%
TCAGGAATTGCTCATTTCTG+TGG + Chr6:113801681-113801700 MsG0680035876.01.T01:intron 40.0%
! AGCTTTTGTGGAACAGGTAA+AGG - Chr6:113801460-113801479 None:intergenic 40.0%
! GATTGTTGCTGTAGCTTTTG+TGG - Chr6:113801472-113801491 None:intergenic 40.0%
! GGCGTTTGTTGAAGAATCAA+TGG + Chr6:113801327-113801346 MsG0680035876.01.T01:exon 40.0%
!! AAAGAGTAATTGACTCGGTG+TGG - Chr6:113801627-113801646 None:intergenic 40.0%
!! ACAGGGGAATGGATTATACA+AGG + Chr6:113802166-113802185 MsG0680035876.01.T01:intron 40.0%
ATATCATTCTCCTCTGGCAG+AGG + Chr6:113801709-113801728 MsG0680035876.01.T01:CDS 45.0%
ATGACGTCACTCCTCCATTA+AGG - Chr6:113801357-113801376 None:intergenic 45.0%
CAGCGTCGTCGTATACTTAT+TGG + Chr6:113801407-113801426 MsG0680035876.01.T01:CDS 45.0%
CTCATCAATTGGGTTCTGCT+TGG + Chr6:113801509-113801528 MsG0680035876.01.T01:CDS 45.0%
GAAGTGGCAACACAAGAGTA+AGG - Chr6:113802077-113802096 None:intergenic 45.0%
GATGTTCCGGAGATCTCATT+TGG + Chr6:113802216-113802235 MsG0680035876.01.T01:CDS 45.0%
GGTTCCATATCATTCTCCTC+TGG + Chr6:113801703-113801722 MsG0680035876.01.T01:CDS 45.0%
TCAATGGAGTCCCTTAATGG+AGG + Chr6:113801343-113801362 MsG0680035876.01.T01:exon 45.0%
TCTGCCAGAGGAGAATGATA+TGG - Chr6:113801710-113801729 None:intergenic 45.0%
TGACGTCACTCCTCCATTAA+GGG - Chr6:113801356-113801375 None:intergenic 45.0%
TTGTACTCGGATCGATCTAG+AGG + Chr6:113801255-113801274 MsG0680035876.01.T01:five_prime_UTR 45.0%
! GGAGATCTCATTTGGTGAAC+TGG + Chr6:113802224-113802243 MsG0680035876.01.T01:CDS 45.0%
! TGAGTGAGTTGAAGTACGAG+CGG + Chr6:113801306-113801325 MsG0680035876.01.T01:five_prime_UTR 45.0%
! TGCTGTAGCTTTTGTGGAAC+AGG - Chr6:113801466-113801485 None:intergenic 45.0%
!! TAATTGACTCGGTGTGGTGT+AGG - Chr6:113801621-113801640 None:intergenic 45.0%
GTCATCGTCGTCGAAATCGA+AGG + Chr6:113801372-113801391 MsG0680035876.01.T01:CDS 50.0%
! TGGTGAACTGGATGACTTGC+AGG + Chr6:113802236-113802255 MsG0680035876.01.T01:CDS 50.0%
! TTGGAATCGTGCTGGCACTT+GGG + Chr6:113801528-113801547 MsG0680035876.01.T01:CDS 50.0%
! TTGGGGGCATATCGATGTTC+CGG + Chr6:113802203-113802222 MsG0680035876.01.T01:CDS 50.0%
GGTTCTGCTTGGAATCGTGC+TGG + Chr6:113801520-113801539 MsG0680035876.01.T01:CDS 55.0%
! CTTGGAATCGTGCTGGCACT+TGG + Chr6:113801527-113801546 MsG0680035876.01.T01:CDS 55.0%
TTGTGCTGTGCCTCTGCCAG+AGG - Chr6:113801722-113801741 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 113801242 113802297 113801242 ID=MsG0680035876.01;Name=MsG0680035876.01
Chr6 mRNA 113801242 113802297 113801242 ID=MsG0680035876.01.T01;Parent=MsG0680035876.01;Name=MsG0680035876.01.T01;_AED=0.50;_eAED=0.53;_QI=104|1|0.75|1|1|1|4|0|176
Chr6 exon 113801242 113801591 113801242 ID=MsG0680035876.01.T01:exon:15327;Parent=MsG0680035876.01.T01
Chr6 exon 113801685 113801771 113801685 ID=MsG0680035876.01.T01:exon:15328;Parent=MsG0680035876.01.T01
Chr6 exon 113801992 113802061 113801992 ID=MsG0680035876.01.T01:exon:15329;Parent=MsG0680035876.01.T01
Chr6 exon 113802170 113802297 113802170 ID=MsG0680035876.01.T01:exon:15330;Parent=MsG0680035876.01.T01
Chr6 five_prime_UTR 113801242 113801345 113801242 ID=MsG0680035876.01.T01:five_prime_utr;Parent=MsG0680035876.01.T01
Chr6 CDS 113801346 113801591 113801346 ID=MsG0680035876.01.T01:cds;Parent=MsG0680035876.01.T01
Chr6 CDS 113801685 113801771 113801685 ID=MsG0680035876.01.T01:cds;Parent=MsG0680035876.01.T01
Chr6 CDS 113801992 113802061 113801992 ID=MsG0680035876.01.T01:cds;Parent=MsG0680035876.01.T01
Chr6 CDS 113802170 113802297 113802170 ID=MsG0680035876.01.T01:cds;Parent=MsG0680035876.01.T01
Gene Sequence

>MsG0680035876.01.T01

ATGGAGTCCCTTAATGGAGGAGTGACGTCATCGTCGTCGAAATCGAAGGAAGAAGAAAAAGCAGCGTCGTCGTATACTTATTGGGTTCGGAATAAAACTGAAGCTGCAGCGCCTTTACCTGTTCCACAAAAGCTACAGCAACAATCTTCTTCTGTTACTTCTTCTCATCAATTGGGTTCTGCTTGGAATCGTGCTGGCACTTGGGAAGAGAAATCTCTTAACCATTGGGCTATTCCTAGAATTAAGGAATTGCTCATTTCTGTGGGTTCCATATCATTCTCCTCTGGCAGAGGCACAGCACAAGTTCAAGATGTAACCAAATGTGTTGGAGATGCACTTGTGGTGGTCGTTCGTAACAAGAAACGTGTCAGTTTCACATATGAGCTGTCCTTAAAAGTGAAAGGGGAATGGATTATACAAGGAAATAAAAAGTTGCTTGGGGGCATATCGATGTTCCGGAGATCTCATTTGGTGAACTGGATGACTTGCAGGTACCTCTTATTATTGATTTTAACACTTTTCAGACACTGA

Protein sequence

>MsG0680035876.01.T01

MESLNGGVTSSSSKSKEEEKAASSYTYWVRNKTEAAAPLPVPQKLQQQSSSVTSSHQLGSAWNRAGTWEEKSLNHWAIPRIKELLISVGSISFSSGRGTAQVQDVTKCVGDALVVVVRNKKRVSFTYELSLKVKGEWIIQGNKKLLGGISMFRRSHLVNWMTCRYLLLLILTLFRH*