Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000255.01.T01 | XP_003589208.1 | 94.737 | 95 | 1 | 1 | 1 | 91 | 1 | 95 | 3.79E-57 | 181 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047957.01 | MsG0180000255.01 | 0.808298 | 3.316982e-50 | 1.889658e-47 |
| MsG0180000114.01 | MsG0180000255.01 | 0.809919 | 1.491289e-50 | 8.868082e-48 |
| MsG0180000251.01 | MsG0180000255.01 | 0.821813 | 3.306473e-53 | 2.718807e-50 |
| MsG0180000255.01 | MsG0180000777.01 | 0.802202 | 6.275330e-49 | 3.055415e-46 |
| MsG0180000255.01 | MsG0180000814.01 | 0.824090 | 9.742433e-54 | 8.544102e-51 |
| MsG0180000255.01 | MsG0180001886.01 | 0.831762 | 1.389324e-55 | 1.522389e-52 |
| MsG0180000255.01 | MsG0180003189.01 | 0.809474 | 1.858948e-50 | 1.092468e-47 |
| MsG0180000255.01 | MsG0180004602.01 | 0.801011 | 1.101021e-48 | 5.202126e-46 |
| MsG0180000255.01 | MsG0180004667.01 | -0.803933 | 2.751932e-49 | 1.400713e-46 |
| MsG0180000255.01 | MsG0180004765.01 | 0.838717 | 2.435665e-57 | 3.290422e-54 |
| MsG0180000255.01 | MsG0180005730.01 | 0.807344 | 5.293238e-50 | 2.941018e-47 |
| MsG0180000255.01 | MsG0180006134.01 | 0.802425 | 5.644671e-49 | 2.764247e-46 |
| MsG0180000255.01 | MsG0280006601.01 | 0.813352 | 2.673087e-51 | 1.741601e-48 |
| MsG0180000255.01 | MsG0280007547.01 | 0.814880 | 1.229484e-51 | 8.348433e-49 |
| MsG0180000255.01 | MsG0280007947.01 | 0.816549 | 5.222652e-52 | 3.711799e-49 |
| MsG0180000255.01 | MsG0280009350.01 | 0.811241 | 7.724487e-51 | 4.756677e-48 |
| MsG0180000255.01 | MsG0280010722.01 | 0.802599 | 5.196571e-49 | 2.556046e-46 |
| MsG0180000255.01 | MsG0280011109.01 | 0.811328 | 7.395068e-51 | 4.564126e-48 |
| MsG0180000255.01 | MsG0380014225.01 | -0.808428 | 3.112114e-50 | 1.779026e-47 |
| MsG0180000255.01 | MsG0380014770.01 | 0.805469 | 1.315161e-49 | 6.963456e-47 |
| MsG0180000255.01 | MsG0380015885.01 | 0.809499 | 1.836084e-50 | 1.079768e-47 |
| MsG0180000255.01 | MsG0380016182.01 | 0.816662 | 4.926048e-52 | 3.512062e-49 |
| MsG0180000255.01 | MsG0380017048.01 | 0.805218 | 1.484315e-49 | 7.808497e-47 |
| MsG0180000255.01 | MsG0380017551.01 | -0.805196 | 1.500465e-49 | 7.888967e-47 |
| MsG0180000255.01 | MsG0480018236.01 | 0.844099 | 9.323304e-59 | 1.489346e-55 |
| MsG0180000255.01 | MsG0480018883.01 | 0.881632 | 2.042618e-70 | 1.205699e-66 |
| MsG0180000255.01 | MsG0480020765.01 | 0.815187 | 1.051105e-51 | 7.196719e-49 |
| MsG0180000255.01 | MsG0480021280.01 | 0.814481 | 1.506899e-51 | 1.012340e-48 |
| MsG0180000255.01 | MsG0580024363.01 | -0.810230 | 1.277881e-50 | 7.660714e-48 |
| MsG0180000255.01 | MsG0580025832.01 | 0.801779 | 7.665022e-49 | 3.692149e-46 |
| MsG0180000255.01 | MsG0580026304.01 | 0.820303 | 7.367030e-53 | 5.807301e-50 |
| MsG0180000255.01 | MsG0580026340.01 | 0.818338 | 2.065035e-52 | 1.541573e-49 |
| MsG0180000255.01 | MsG0580029236.01 | 0.800325 | 1.519940e-48 | 7.057204e-46 |
| MsG0180000255.01 | MsG0580029376.01 | 0.802050 | 6.743755e-49 | 3.270906e-46 |
| MsG0180000255.01 | MsG0580029999.01 | -0.834283 | 3.278698e-56 | 3.873005e-53 |
| MsG0180000255.01 | MsG0680031334.01 | 0.812515 | 4.078725e-51 | 2.598657e-48 |
| MsG0180000255.01 | MsG0680031621.01 | 0.807317 | 5.361077e-50 | 2.976799e-47 |
| MsG0180000255.01 | MsG0680031815.01 | 0.818173 | 2.250023e-52 | 1.671967e-49 |
| MsG0180000255.01 | MsG0680032058.01 | 0.814928 | 1.199689e-51 | 8.156596e-49 |
| MsG0180000255.01 | MsG0680032460.01 | -0.804423 | 2.176040e-49 | 1.121485e-46 |
| MsG0180000255.01 | MsG0680035707.01 | 0.828267 | 9.888327e-55 | 9.778998e-52 |
| MsG0180000255.01 | MsG0780036593.01 | 0.801378 | 9.260330e-49 | 4.415740e-46 |
| MsG0180000255.01 | MsG0780036978.01 | 0.812611 | 3.885675e-51 | 2.481880e-48 |
| MsG0180000255.01 | MsG0780037284.01 | -0.808565 | 2.910030e-50 | 1.669370e-47 |
| MsG0180000255.01 | MsG0780038907.01 | 0.813306 | 2.736661e-51 | 1.780727e-48 |
| MsG0180000255.01 | MsG0780039170.01 | 0.801392 | 9.200422e-49 | 4.388635e-46 |
| MsG0180000255.01 | MsG0780039222.01 | -0.808690 | 2.736447e-50 | 1.574899e-47 |
| MsG0180000255.01 | MsG0780039867.01 | -0.819238 | 1.289965e-52 | 9.871480e-50 |
| MsG0180000255.01 | MsG0780040888.01 | 0.851915 | 6.502661e-61 | 1.336145e-57 |
| MsG0180000255.01 | MsG0780041015.01 | -0.825108 | 5.609099e-54 | 5.064905e-51 |
| MsG0180000255.01 | MsG0880042622.01 | 0.805651 | 1.204194e-49 | 6.405922e-47 |
| MsG0180000255.01 | MsG0880043157.01 | 0.817829 | 2.692017e-52 | 1.981628e-49 |
| MsG0180000255.01 | MsG0880043509.01 | 0.806507 | 7.954343e-50 | 4.325013e-47 |
| MsG0180000255.01 | MsG0880043941.01 | 0.802514 | 5.411104e-49 | 2.655683e-46 |
| MsG0180000255.01 | MsG0880045362.01 | 0.800466 | 1.422661e-48 | 6.628695e-46 |
| MsG0180000255.01 | MsG0880045535.01 | 0.804847 | 1.774453e-49 | 9.246877e-47 |
| MsG0180000255.01 | MsG0880045765.01 | 0.815699 | 8.085875e-52 | 5.614987e-49 |
| MsG0180000255.01 | MsG0880045992.01 | 0.803137 | 4.023021e-49 | 2.006076e-46 |
| MsG0180000255.01 | MsG0880046457.01 | 0.817049 | 4.033303e-52 | 2.906266e-49 |
| MsG0180000255.01 | MsG0880046790.01 | 0.825404 | 4.774937e-54 | 4.348098e-51 |
| MsG0180000255.01 | MsG0880047045.01 | 0.812874 | 3.402814e-51 | 2.188461e-48 |
| MsG0180000255.01 | MsG0880047046.01 | 0.807459 | 5.002343e-50 | 2.787845e-47 |
| MsG0180000255.01 | MsG0880047052.01 | 0.809901 | 1.504621e-50 | 8.943040e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000255.01.T01 | MTR_1g019640 | 94.737 | 95 | 1 | 1 | 1 | 91 | 1 | 95 | 4.59e-61 | 181 |
| MsG0180000255.01.T01 | MTR_1g019660 | 89.474 | 95 | 6 | 1 | 1 | 91 | 1 | 95 | 4.05e-55 | 166 |
| MsG0180000255.01.T01 | MTR_1g019610 | 86.316 | 95 | 9 | 1 | 1 | 91 | 1 | 95 | 2.02e-54 | 164 |
| MsG0180000255.01.T01 | MTR_1g019670 | 65.591 | 93 | 29 | 1 | 1 | 90 | 1 | 93 | 1.53e-38 | 124 |
| MsG0180000255.01.T01 | MTR_1g019600 | 45.000 | 100 | 46 | 1 | 1 | 91 | 1 | 100 | 3.30e-19 | 75.5 |
| MsG0180000255.01.T01 | MTR_1g019590 | 43.678 | 87 | 45 | 1 | 5 | 91 | 21 | 103 | 2.65e-16 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTGATTAGTCCGTCTTTAT+TGG | 0.241517 | 1:+3434913 | None:intergenic |
| TATCTCCTTACCCGTGGTTT+TGG | 0.273881 | 1:-3434863 | MsG0180000255.01.T01:CDS |
| TGATCCATAGACACCCTCTT+TGG | 0.332546 | 1:+3435091 | None:intergenic |
| TATCCCAAAGAGGGTGTCTA+TGG | 0.383107 | 1:-3435095 | MsG0180000255.01.T01:CDS |
| GATCCATAGACACCCTCTTT+GGG | 0.423736 | 1:+3435092 | None:intergenic |
| CAACGATTGGCTCTCCATCC+AGG | 0.437823 | 1:+3434953 | None:intergenic |
| AGCTTTCATCAAACAACGAT+TGG | 0.498009 | 1:+3434940 | None:intergenic |
| TCTTGCCAAAACCACGGGTA+AGG | 0.519368 | 1:+3434858 | None:intergenic |
| CGATGGTCGTTACACAAAGA+AGG | 0.521782 | 1:-3435014 | MsG0180000255.01.T01:CDS |
| TAGGTTCTTACTACCCTGGA+TGG | 0.528156 | 1:-3434967 | MsG0180000255.01.T01:CDS |
| TTAGTTCTTGCCAAAACCAC+GGG | 0.528157 | 1:+3434853 | None:intergenic |
| TGGATCAAATCAACTTTGAA+AGG | 0.536831 | 1:-3435075 | MsG0180000255.01.T01:CDS |
| GATTTAGGTTCTTACTACCC+TGG | 0.543944 | 1:-3434971 | MsG0180000255.01.T01:CDS |
| AACGATTGGCTCTCCATCCA+GGG | 0.580860 | 1:+3434954 | None:intergenic |
| ATTAGTTCTTGCCAAAACCA+CGG | 0.592102 | 1:+3434852 | None:intergenic |
| GCCTGTGTTTATCCCAAAGA+GGG | 0.602626 | 1:-3435104 | MsG0180000255.01.T01:CDS |
| ATTGATTATCTCCTTACCCG+TGG | 0.619921 | 1:-3434869 | MsG0180000255.01.T01:CDS |
| AAAGCTGATGCCAATAAAGA+CGG | 0.628000 | 1:-3434923 | MsG0180000255.01.T01:CDS |
| TGCCTGTGTTTATCCCAAAG+AGG | 0.630941 | 1:-3435105 | MsG0180000255.01.T01:CDS |
| ACCCTCTTTGGGATAAACAC+AGG | 0.645492 | 1:+3435103 | None:intergenic |
| AGACGGACTAATCAGTGGCG+AGG | 0.659853 | 1:-3434906 | MsG0180000255.01.T01:CDS |
| AATAAAGACGGACTAATCAG+TGG | 0.668016 | 1:-3434911 | MsG0180000255.01.T01:CDS |
| AAGGCGGATATCAATAACGA+TGG | 0.697636 | 1:-3435031 | MsG0180000255.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGGAACATTATAGAAAAAT+TGG | - | Chr1:3434903-3434922 | MsG0180000255.01.T01:CDS | 20.0% |
| ! | CATTATAGAAAAATTGGAGA+AGG | - | Chr1:3434909-3434928 | MsG0180000255.01.T01:CDS | 25.0% |
| !!! | AAAAAAGCTCTAAAGGATTT+AGG | - | Chr1:3434973-3434992 | MsG0180000255.01.T01:CDS | 25.0% |
| TATAGAAAAATTGGAGAAGG+CGG | - | Chr1:3434912-3434931 | MsG0180000255.01.T01:CDS | 30.0% | |
| TGGATCAAATCAACTTTGAA+AGG | - | Chr1:3434884-3434903 | MsG0180000255.01.T01:CDS | 30.0% | |
| AAAGCTGATGCCAATAAAGA+CGG | - | Chr1:3435036-3435055 | MsG0180000255.01.T01:CDS | 35.0% | |
| AGCTTTCATCAAACAACGAT+TGG | + | Chr1:3435022-3435041 | None:intergenic | 35.0% | |
| ! | ACTGATTAGTCCGTCTTTAT+TGG | + | Chr1:3435049-3435068 | None:intergenic | 35.0% |
| ! | GGAACTCAAAAAAGCTCTAA+AGG | - | Chr1:3434966-3434985 | MsG0180000255.01.T01:CDS | 35.0% |
| !! | AATAAAGACGGACTAATCAG+TGG | - | Chr1:3435048-3435067 | MsG0180000255.01.T01:CDS | 35.0% |
| AAGGCGGATATCAATAACGA+TGG | - | Chr1:3434928-3434947 | MsG0180000255.01.T01:CDS | 40.0% | |
| ATTGATTATCTCCTTACCCG+TGG | - | Chr1:3435090-3435109 | MsG0180000255.01.T01:CDS | 40.0% | |
| ! | GATTTAGGTTCTTACTACCC+TGG | - | Chr1:3434988-3435007 | MsG0180000255.01.T01:CDS | 40.0% |
| ! | TTAGTTCTTGCCAAAACCAC+GGG | + | Chr1:3435109-3435128 | None:intergenic | 40.0% |
| CGATGGTCGTTACACAAAGA+AGG | - | Chr1:3434945-3434964 | MsG0180000255.01.T01:CDS | 45.0% | |
| GATCCATAGACACCCTCTTT+GGG | + | Chr1:3434870-3434889 | None:intergenic | 45.0% | |
| GCCTGTGTTTATCCCAAAGA+GGG | - | Chr1:3434855-3434874 | MsG0180000255.01.T01:CDS | 45.0% | |
| TAGGTTCTTACTACCCTGGA+TGG | - | Chr1:3434992-3435011 | MsG0180000255.01.T01:CDS | 45.0% | |
| TGATCCATAGACACCCTCTT+TGG | + | Chr1:3434871-3434890 | None:intergenic | 45.0% | |
| TGCCTGTGTTTATCCCAAAG+AGG | - | Chr1:3434854-3434873 | MsG0180000255.01.T01:CDS | 45.0% | |
| ! | TATCTCCTTACCCGTGGTTT+TGG | - | Chr1:3435096-3435115 | MsG0180000255.01.T01:CDS | 45.0% |
| !! | ACCCTCTTTGGGATAAACAC+AGG | + | Chr1:3434859-3434878 | None:intergenic | 45.0% |
| !! | TATCCCAAAGAGGGTGTCTA+TGG | - | Chr1:3434864-3434883 | MsG0180000255.01.T01:CDS | 45.0% |
| TCTTGCCAAAACCACGGGTA+AGG | + | Chr1:3435104-3435123 | None:intergenic | 50.0% | |
| ! | AACGATTGGCTCTCCATCCA+GGG | + | Chr1:3435008-3435027 | None:intergenic | 50.0% |
| ! | CAACGATTGGCTCTCCATCC+AGG | + | Chr1:3435009-3435028 | None:intergenic | 55.0% |
| !! | AGACGGACTAATCAGTGGCG+AGG | - | Chr1:3435053-3435072 | MsG0180000255.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 3434853 | 3435128 | 3434853 | ID=MsG0180000255.01;Name=MsG0180000255.01 |
| Chr1 | mRNA | 3434853 | 3435128 | 3434853 | ID=MsG0180000255.01.T01;Parent=MsG0180000255.01;Name=MsG0180000255.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|91 |
| Chr1 | exon | 3434853 | 3435128 | 3434853 | ID=MsG0180000255.01.T01:exon:9982;Parent=MsG0180000255.01.T01 |
| Chr1 | CDS | 3434853 | 3435128 | 3434853 | ID=MsG0180000255.01.T01:cds;Parent=MsG0180000255.01.T01 |
| Gene Sequence |
| Protein sequence |
