Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008756.01.T01 | XP_003595594.1 | 92.647 | 68 | 5 | 0 | 1 | 68 | 1 | 68 | 1.65E-34 | 132 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008756.01.T01 | Q6ZLP5 | 70.175 | 57 | 17 | 0 | 12 | 68 | 2 | 58 | 1.11E-21 | 89.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008756.01.T01 | G7IIY1 | 92.647 | 68 | 5 | 0 | 1 | 68 | 1 | 68 | 7.90e-35 | 132 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003744.01 | MsG0280008756.01 | 0.824676 | 7.094095e-54 | 6.327796e-51 |
| MsG0180004056.01 | MsG0280008756.01 | 0.826220 | 3.056578e-54 | 2.848792e-51 |
| MsG0180005407.01 | MsG0280008756.01 | 0.818425 | 1.973789e-52 | 1.477017e-49 |
| MsG0180006098.01 | MsG0280008756.01 | 0.821452 | 4.008630e-53 | 3.262380e-50 |
| MsG0280007109.01 | MsG0280008756.01 | 0.816694 | 4.845900e-52 | 3.457748e-49 |
| MsG0280007948.01 | MsG0280008756.01 | 0.827653 | 1.389549e-54 | 1.349769e-51 |
| MsG0280008041.01 | MsG0280008756.01 | 0.804916 | 1.716650e-49 | 8.961768e-47 |
| MsG0280008756.01 | MsG0280008758.01 | 0.859503 | 3.953831e-63 | 1.046499e-59 |
| MsG0280008756.01 | MsG0280010447.01 | 0.803929 | 2.756661e-49 | 1.403000e-46 |
| MsG0280008756.01 | MsG0280010567.01 | 0.809104 | 2.231765e-50 | 1.298715e-47 |
| MsG0280008756.01 | MsG0280011307.01 | 0.863309 | 2.728674e-64 | 8.235489e-61 |
| MsG0280008756.01 | MsG0380014365.01 | 0.803270 | 3.776022e-49 | 1.889366e-46 |
| MsG0280008756.01 | MsG0380014541.01 | 0.835463 | 1.653443e-56 | 2.023423e-53 |
| MsG0280008756.01 | MsG0380014561.01 | 0.807209 | 5.651766e-50 | 3.129333e-47 |
| MsG0280008756.01 | MsG0380015564.01 | 0.812437 | 4.242599e-51 | 2.697612e-48 |
| MsG0280008756.01 | MsG0380015922.01 | 0.827548 | 1.472220e-54 | 1.425780e-51 |
| MsG0280008756.01 | MsG0380016604.01 | 0.806118 | 9.605375e-50 | 5.171321e-47 |
| MsG0280008756.01 | MsG0380017403.01 | 0.800047 | 1.730876e-48 | 7.979804e-46 |
| MsG0280008756.01 | MsG0480020416.01 | 0.821776 | 3.372001e-53 | 2.769657e-50 |
| MsG0280008756.01 | MsG0480021240.01 | 0.810878 | 9.257329e-51 | 5.646456e-48 |
| MsG0280008756.01 | MsG0480023224.01 | 0.823010 | 1.743762e-53 | 1.483463e-50 |
| MsG0280008756.01 | MsG0580024618.01 | 0.800086 | 1.699528e-48 | 7.843115e-46 |
| MsG0280008756.01 | MsG0580025115.01 | 0.831822 | 1.342543e-55 | 1.473750e-52 |
| MsG0280008756.01 | MsG0580025489.01 | 0.811506 | 6.767786e-51 | 4.196946e-48 |
| MsG0280008756.01 | MsG0580026225.01 | 0.833800 | 4.331699e-56 | 5.043265e-53 |
| MsG0280008756.01 | MsG0580027462.01 | 0.802537 | 5.351722e-49 | 2.628190e-46 |
| MsG0280008756.01 | MsG0580028185.01 | 0.826033 | 3.387414e-54 | 3.140338e-51 |
| MsG0280008756.01 | MsG0580029658.01 | 0.850568 | 1.560643e-60 | 3.069992e-57 |
| MsG0280008756.01 | MsG0580029661.01 | 0.817723 | 2.844008e-52 | 2.087473e-49 |
| MsG0280008756.01 | MsG0580030249.01 | 0.805188 | 1.506160e-49 | 7.917298e-47 |
| MsG0280008756.01 | MsG0680030841.01 | 0.808927 | 2.434744e-50 | 1.410060e-47 |
| MsG0280008756.01 | MsG0680031828.01 | 0.838476 | 2.811022e-57 | 3.769504e-54 |
| MsG0280008756.01 | MsG0680035476.01 | 0.814518 | 1.478882e-51 | 9.945193e-49 |
| MsG0280008756.01 | MsG0780038911.01 | 0.822672 | 2.089824e-53 | 1.760923e-50 |
| MsG0280008756.01 | MsG0780040312.01 | 0.807018 | 6.204659e-50 | 3.418315e-47 |
| MsG0280008756.01 | MsG0780041754.01 | 0.821735 | 3.447491e-53 | 2.828208e-50 |
| MsG0280008756.01 | MsG0880042052.01 | 0.810385 | 1.183619e-50 | 7.124274e-48 |
| MsG0280008756.01 | MsG0880044075.01 | 0.802586 | 5.228344e-49 | 2.570807e-46 |
| MsG0280008756.01 | MsG0880045106.01 | 0.815585 | 8.572232e-52 | 5.934091e-49 |
| MsG0280008756.01 | MsG0880046047.01 | 0.840899 | 6.586529e-58 | 9.517167e-55 |
| MsG0280008756.01 | MsG0880046068.01 | 0.804189 | 2.434537e-49 | 1.247214e-46 |
| MsG0280008756.01 | MsG0880046077.01 | 0.849257 | 3.631113e-60 | 6.844349e-57 |
| MsG0280008756.01 | MsG0880046089.01 | 0.807030 | 6.166697e-50 | 3.398571e-47 |
| MsG0280008756.01 | MsG0880046214.01 | 0.819006 | 1.456910e-52 | 1.107901e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008756.01.T01 | MTR_2g049790 | 92.647 | 68 | 5 | 0 | 1 | 68 | 1 | 68 | 2.00e-38 | 132 |
| MsG0280008756.01.T01 | MTR_5g088350 | 67.308 | 52 | 17 | 0 | 17 | 68 | 7 | 58 | 3.63e-19 | 79.0 |
| MsG0280008756.01.T01 | MTR_5g088350 | 67.308 | 52 | 17 | 0 | 17 | 68 | 7 | 58 | 6.99e-19 | 79.0 |
| MsG0280008756.01.T01 | MTR_4g114670 | 63.462 | 52 | 19 | 0 | 17 | 68 | 5 | 56 | 3.29e-18 | 76.6 |
| MsG0280008756.01.T01 | MTR_4g114670 | 63.462 | 52 | 19 | 0 | 17 | 68 | 5 | 56 | 3.59e-18 | 77.0 |
| MsG0280008756.01.T01 | MTR_5g088350 | 67.308 | 52 | 17 | 0 | 17 | 68 | 7 | 58 | 3.64e-18 | 76.6 |
| MsG0280008756.01.T01 | MTR_4g114670 | 63.462 | 52 | 19 | 0 | 17 | 68 | 5 | 56 | 3.88e-18 | 76.6 |
| MsG0280008756.01.T01 | MTR_8g024590 | 58.929 | 56 | 23 | 0 | 13 | 68 | 7 | 62 | 4.99e-18 | 76.3 |
| MsG0280008756.01.T01 | MTR_5g067150 | 66.000 | 50 | 17 | 0 | 19 | 68 | 5 | 54 | 5.30e-18 | 76.3 |
| MsG0280008756.01.T01 | MTR_5g067150 | 66.000 | 50 | 17 | 0 | 19 | 68 | 5 | 54 | 9.03e-18 | 75.5 |
| MsG0280008756.01.T01 | MTR_5g067150 | 66.000 | 50 | 17 | 0 | 19 | 68 | 5 | 54 | 1.32e-17 | 75.1 |
| MsG0280008756.01.T01 | MTR_8g024600 | 61.538 | 52 | 20 | 0 | 17 | 68 | 11 | 62 | 1.69e-17 | 75.1 |
| MsG0280008756.01.T01 | MTR_1g103760 | 54.717 | 53 | 24 | 0 | 16 | 68 | 5 | 57 | 1.00e-12 | 61.6 |
| MsG0280008756.01.T01 | MTR_4g128820 | 54.000 | 50 | 23 | 0 | 21 | 70 | 8 | 57 | 2.28e-12 | 60.5 |
| MsG0280008756.01.T01 | MTR_3g072320 | 50.000 | 52 | 26 | 0 | 16 | 67 | 5 | 56 | 2.15e-11 | 57.8 |
| MsG0280008756.01.T01 | MTR_2g045470 | 58.696 | 46 | 19 | 0 | 15 | 60 | 12 | 57 | 2.48e-11 | 57.4 |
| MsG0280008756.01.T01 | MTR_4g131060 | 56.250 | 48 | 21 | 0 | 21 | 68 | 16 | 63 | 3.15e-11 | 57.0 |
| MsG0280008756.01.T01 | MTR_7g072575 | 50.943 | 53 | 26 | 0 | 16 | 68 | 5 | 57 | 6.77e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008756.01.T01 | AT1G30270 | 77.551 | 49 | 11 | 0 | 20 | 68 | 28 | 76 | 9.73e-21 | 84.3 |
| MsG0280008756.01.T01 | AT1G30270 | 77.551 | 49 | 11 | 0 | 20 | 68 | 28 | 76 | 1.07e-20 | 84.0 |
| MsG0280008756.01.T01 | AT2G26980 | 69.231 | 52 | 16 | 0 | 17 | 68 | 8 | 59 | 3.57e-20 | 80.9 |
| MsG0280008756.01.T01 | AT2G26980 | 69.231 | 52 | 16 | 0 | 17 | 68 | 8 | 59 | 2.20e-19 | 80.1 |
| MsG0280008756.01.T01 | AT2G26980 | 69.231 | 52 | 16 | 0 | 17 | 68 | 8 | 59 | 2.48e-19 | 80.1 |
| MsG0280008756.01.T01 | AT2G26980 | 69.231 | 52 | 16 | 0 | 17 | 68 | 8 | 59 | 6.59e-19 | 79.0 |
| MsG0280008756.01.T01 | AT2G26980 | 69.231 | 52 | 16 | 0 | 17 | 68 | 18 | 69 | 6.84e-19 | 79.0 |
| MsG0280008756.01.T01 | AT1G01140 | 53.968 | 63 | 29 | 0 | 6 | 68 | 2 | 64 | 8.69e-19 | 78.6 |
| MsG0280008756.01.T01 | AT1G01140 | 53.968 | 63 | 29 | 0 | 6 | 68 | 2 | 64 | 8.93e-19 | 78.6 |
| MsG0280008756.01.T01 | AT1G01140 | 53.968 | 63 | 29 | 0 | 6 | 68 | 2 | 64 | 9.07e-19 | 78.6 |
| MsG0280008756.01.T01 | AT4G24400 | 64.000 | 50 | 18 | 0 | 19 | 68 | 5 | 54 | 5.83e-17 | 73.6 |
| MsG0280008756.01.T01 | AT5G35410 | 64.000 | 50 | 18 | 0 | 19 | 68 | 7 | 56 | 7.01e-17 | 73.2 |
| MsG0280008756.01.T01 | AT5G35410 | 64.000 | 50 | 18 | 0 | 19 | 68 | 7 | 56 | 7.61e-17 | 73.2 |
| MsG0280008756.01.T01 | AT4G24400 | 64.000 | 50 | 18 | 0 | 19 | 68 | 5 | 54 | 8.74e-17 | 72.8 |
| MsG0280008756.01.T01 | AT5G21326 | 64.000 | 50 | 18 | 0 | 19 | 68 | 9 | 58 | 1.96e-16 | 72.0 |
| MsG0280008756.01.T01 | AT5G21222 | 57.692 | 52 | 22 | 0 | 17 | 68 | 7 | 58 | 1.03e-13 | 64.3 |
| MsG0280008756.01.T01 | AT5G21222 | 57.692 | 52 | 22 | 0 | 17 | 68 | 7 | 58 | 1.03e-13 | 64.3 |
| MsG0280008756.01.T01 | AT5G21222 | 57.692 | 52 | 22 | 0 | 17 | 68 | 7 | 58 | 1.03e-13 | 64.3 |
| MsG0280008756.01.T01 | AT5G21222 | 57.692 | 52 | 22 | 0 | 17 | 68 | 7 | 58 | 1.03e-13 | 64.3 |
| MsG0280008756.01.T01 | AT5G01810 | 59.574 | 47 | 19 | 0 | 22 | 68 | 11 | 57 | 6.02e-13 | 62.0 |
| MsG0280008756.01.T01 | AT5G01810 | 59.574 | 47 | 19 | 0 | 22 | 68 | 11 | 57 | 6.02e-13 | 62.0 |
| MsG0280008756.01.T01 | AT5G01810 | 59.574 | 47 | 19 | 0 | 22 | 68 | 11 | 57 | 6.25e-13 | 62.0 |
| MsG0280008756.01.T01 | AT1G29230 | 54.167 | 48 | 22 | 0 | 21 | 68 | 72 | 119 | 8.88e-13 | 61.6 |
| MsG0280008756.01.T01 | AT5G45810 | 56.250 | 48 | 21 | 0 | 21 | 68 | 26 | 73 | 2.07e-12 | 60.5 |
| MsG0280008756.01.T01 | AT4G18700 | 54.167 | 48 | 22 | 0 | 21 | 68 | 24 | 71 | 6.82e-12 | 59.3 |
| MsG0280008756.01.T01 | AT5G45820 | 57.447 | 47 | 20 | 0 | 22 | 68 | 11 | 57 | 8.81e-12 | 58.9 |
| MsG0280008756.01.T01 | AT4G14580 | 48.333 | 60 | 27 | 1 | 16 | 71 | 14 | 73 | 1.09e-11 | 58.5 |
| MsG0280008756.01.T01 | AT5G57630 | 50.943 | 53 | 26 | 0 | 16 | 68 | 5 | 57 | 1.21e-11 | 58.5 |
| MsG0280008756.01.T01 | AT5G57630 | 50.943 | 53 | 26 | 0 | 16 | 68 | 5 | 57 | 1.41e-11 | 58.2 |
| MsG0280008756.01.T01 | AT3G23000 | 35.616 | 73 | 43 | 1 | 3 | 71 | 5 | 77 | 1.69e-11 | 58.2 |
| MsG0280008756.01.T01 | AT3G01090 | 46.970 | 66 | 35 | 0 | 3 | 68 | 22 | 87 | 2.30e-11 | 57.8 |
| MsG0280008756.01.T01 | AT3G17510 | 63.415 | 41 | 15 | 0 | 19 | 59 | 16 | 56 | 3.47e-11 | 57.0 |
| MsG0280008756.01.T01 | AT2G34180 | 60.000 | 40 | 16 | 0 | 22 | 61 | 56 | 95 | 6.40e-11 | 56.2 |
| MsG0280008756.01.T01 | AT3G29160 | 48.387 | 62 | 32 | 0 | 7 | 68 | 4 | 65 | 8.18e-11 | 56.2 |
| MsG0280008756.01.T01 | AT3G29160 | 48.387 | 62 | 32 | 0 | 7 | 68 | 4 | 65 | 8.18e-11 | 56.2 |
Find 16 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGCGAGACACGTGGAAAC+TGG | 0.431235 | 2:-37752615 | MsG0280008756.01.T01:CDS |
| CAAGCATAAAATGATTCGTC+AGG | 0.448170 | 2:-37752550 | MsG0280008756.01.T01:CDS |
| CAACGGCGGACGTGCGAAGG+CGG | 0.465088 | 2:-37752691 | MsG0280008756.01.T01:CDS |
| CAGCAACGGCGGACGTGCGA+AGG | 0.469154 | 2:-37752694 | MsG0280008756.01.T01:CDS |
| TATGAGCTGGGTAGGACGTT+AGG | 0.472521 | 2:-37752663 | MsG0280008756.01.T01:CDS |
| AGCAACGGAGGCAGCAGCAA+CGG | 0.481688 | 2:-37752708 | MsG0280008756.01.T01:CDS |
| AACGGCGGACGTGCGAAGGC+GGG | 0.484040 | 2:-37752690 | MsG0280008756.01.T01:CDS |
| CGGGAAAGTATGAGCTGGGT+AGG | 0.549445 | 2:-37752671 | MsG0280008756.01.T01:CDS |
| GAAGGCGGGAAAGTATGAGC+TGG | 0.568172 | 2:-37752676 | MsG0280008756.01.T01:CDS |
| AACGGAGGCAGCAGCAACGG+CGG | 0.578341 | 2:-37752705 | MsG0280008756.01.T01:CDS |
| AAGGCGGGAAAGTATGAGCT+GGG | 0.606003 | 2:-37752675 | MsG0280008756.01.T01:CDS |
| CTGGGTAGGACGTTAGGTGA+AGG | 0.611905 | 2:-37752657 | MsG0280008756.01.T01:CDS |
| GGTGAAGTTTGCGAGACACG+TGG | 0.612290 | 2:-37752622 | MsG0280008756.01.T01:CDS |
| AGGTGAAGGTAATTTCGCGA+AGG | 0.640213 | 2:-37752643 | MsG0280008756.01.T01:CDS |
| TCACGTTCATCGAATAGCAA+CGG | 0.671573 | 2:-37752723 | MsG0280008756.01.T01:CDS |
| CGTTCATCGAATAGCAACGG+AGG | 0.695102 | 2:-37752720 | MsG0280008756.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CAAGCATAAAATGATTCGTC+AGG | - | Chr2:37752712-37752731 | MsG0280008756.01.T01:CDS | 35.0% | |
| TCACGTTCATCGAATAGCAA+CGG | - | Chr2:37752539-37752558 | MsG0280008756.01.T01:CDS | 40.0% | |
| AGGTGAAGGTAATTTCGCGA+AGG | - | Chr2:37752619-37752638 | MsG0280008756.01.T01:CDS | 45.0% | |
| CGTTCATCGAATAGCAACGG+AGG | - | Chr2:37752542-37752561 | MsG0280008756.01.T01:CDS | 50.0% | |
| TATGAGCTGGGTAGGACGTT+AGG | - | Chr2:37752599-37752618 | MsG0280008756.01.T01:CDS | 50.0% | |
| TTTGCGAGACACGTGGAAAC+TGG | - | Chr2:37752647-37752666 | MsG0280008756.01.T01:CDS | 50.0% | |
| ! | AAGGCGGGAAAGTATGAGCT+GGG | - | Chr2:37752587-37752606 | MsG0280008756.01.T01:CDS | 50.0% |
| CTGGGTAGGACGTTAGGTGA+AGG | - | Chr2:37752605-37752624 | MsG0280008756.01.T01:CDS | 55.0% | |
| GGTGAAGTTTGCGAGACACG+TGG | - | Chr2:37752640-37752659 | MsG0280008756.01.T01:CDS | 55.0% | |
| ! | CGGGAAAGTATGAGCTGGGT+AGG | - | Chr2:37752591-37752610 | MsG0280008756.01.T01:CDS | 55.0% |
| ! | GAAGGCGGGAAAGTATGAGC+TGG | - | Chr2:37752586-37752605 | MsG0280008756.01.T01:CDS | 55.0% |
| AGCAACGGAGGCAGCAGCAA+CGG | - | Chr2:37752554-37752573 | MsG0280008756.01.T01:CDS | 60.0% | |
| AACGGAGGCAGCAGCAACGG+CGG | - | Chr2:37752557-37752576 | MsG0280008756.01.T01:CDS | 65.0% | |
| AACGGCGGACGTGCGAAGGC+GGG | - | Chr2:37752572-37752591 | MsG0280008756.01.T01:CDS | 70.0% | |
| CAACGGCGGACGTGCGAAGG+CGG | - | Chr2:37752571-37752590 | MsG0280008756.01.T01:CDS | 70.0% | |
| CAGCAACGGCGGACGTGCGA+AGG | - | Chr2:37752568-37752587 | MsG0280008756.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 37752533 | 37752751 | 37752533 | ID=MsG0280008756.01;Name=MsG0280008756.01 |
| Chr2 | mRNA | 37752533 | 37752751 | 37752533 | ID=MsG0280008756.01.T01;Parent=MsG0280008756.01;Name=MsG0280008756.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|72 |
| Chr2 | exon | 37752533 | 37752751 | 37752533 | ID=MsG0280008756.01.T01:exon:36453;Parent=MsG0280008756.01.T01 |
| Chr2 | CDS | 37752533 | 37752751 | 37752533 | ID=MsG0280008756.01.T01:cds;Parent=MsG0280008756.01.T01 |
| Gene Sequence |
| Protein sequence |
