AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580030249.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580030249.01.T01 MTR_2g009600 94.416 197 11 0 1 197 1 197 1.81e-142 395
MsG0580030249.01.T01 MTR_4g093560 68.208 173 54 1 3 175 2 173 5.75e-87 254
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580030249.01.T01 AT2G14110 67.391 184 60 0 4 187 2 185 2.66e-95 275
MsG0580030249.01.T01 AT2G14110 69.173 133 41 0 4 136 2 134 1.61e-70 211

Find 50 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCATATAGGTCTCTAAAA+TGG 0.182821 5:+108919543 MsG0580030249.01.T01:intron
AGTGCTTTAATAAATATTTA+TGG 0.205351 5:-108918105 None:intergenic
AATGGGAGTGACTAGCATTT+TGG 0.255669 5:+108919561 MsG0580030249.01.T01:CDS
AGATCGAAAACAACGAGTTT+TGG 0.286511 5:-108918295 None:intergenic
TTACCTCTGGCTTGTGTACT+TGG 0.288727 5:+108919864 MsG0580030249.01.T01:three_prime_UTR
GTTGATAATGGGGTAAATCT+TGG 0.322461 5:+108919583 MsG0580030249.01.T01:CDS
TGCTTCGTTCTTTGTTGCTC+AGG 0.352190 5:+108918495 MsG0580030249.01.T01:CDS
ATCTGGCTTCTTAGAATAAT+CGG 0.355976 5:-108919680 None:intergenic
TACTTGGACACTGAATTTGA+TGG 0.360359 5:-108919718 None:intergenic
CAGAGAATTCATTCAACTAC+TGG 0.369761 5:+108918640 MsG0580030249.01.T01:CDS
GGGGCAGGGTTGGAAGTATC+TGG 0.390571 5:-108919697 None:intergenic
CACAAAAGAACAAGCAGAAA+TGG 0.391792 5:+108919653 MsG0580030249.01.T01:CDS
GCTCAATCAAATTAATCGTT+GGG 0.398355 5:+108918181 MsG0580030249.01.T01:five_prime_UTR
CGCTCAATCAAATTAATCGT+TGG 0.422131 5:+108918180 MsG0580030249.01.T01:five_prime_UTR
TCACGCCATGGGAATCTTGT+TGG 0.443268 5:+108918384 MsG0580030249.01.T01:CDS
GAGCAGAGAGAAAGCGAAAT+GGG 0.444666 5:+108918211 MsG0580030249.01.T01:exon
AGAGCAGAGAGAAAGCGAAA+TGG 0.444737 5:+108918210 MsG0580030249.01.T01:exon
TTCATATAGGTCTCTAAAAT+GGG 0.446794 5:+108919544 MsG0580030249.01.T01:intron
GACACTGAATTTGATGGGGC+AGG 0.450210 5:-108919712 None:intergenic
TTCAGGAAATATATTCGAGT+TGG 0.451128 5:+108918596 MsG0580030249.01.T01:intron
CAAGCCAGAGGTAACATGTT+TGG 0.455417 5:-108919855 None:intergenic
TCGATCTCGATTACACTCTC+TGG 0.456392 5:+108918311 MsG0580030249.01.T01:CDS
GAGGTAACATGTTTGGCCTT+CGG 0.467860 5:-108919848 None:intergenic
TGAATTTGATGGGGCAGGGT+TGG 0.470475 5:-108919707 None:intergenic
ACTTGGACACTGAATTTGAT+GGG 0.475320 5:-108919717 None:intergenic
GTTATGATCATCGATGTTGA+TGG 0.481888 5:-108918129 None:intergenic
AAGCGAAATGGGAGAGTCTA+CGG 0.501518 5:+108918222 MsG0580030249.01.T01:exon
CTGTGTAAGACCTTGAGATA+AGG 0.502192 5:-108919608 None:intergenic
ATTGAGACTACAATTCAAGT+AGG 0.515093 5:-108919818 None:intergenic
GATCGACACTCGCAGTAGAA+AGG 0.521923 5:-108918334 None:intergenic
TTCTCTATATCCTCACGCCA+TGG 0.522583 5:+108918372 MsG0580030249.01.T01:CDS
TAGAGAAGGAGTTTCGCGCT+TGG 0.523146 5:-108918356 None:intergenic
GCTTTAGCAATATCCGCAGT+TGG 0.525120 5:-108918451 None:intergenic
TTCGAGTTGGACTCATAAAA+CGG 0.526393 5:+108918609 MsG0580030249.01.T01:CDS
TCTCTATATCCTCACGCCAT+GGG 0.543540 5:+108918373 MsG0580030249.01.T01:CDS
AAGGCCAAACATGTTACCTC+TGG 0.557299 5:+108919851 MsG0580030249.01.T01:three_prime_UTR
CTCAATCAAATTAATCGTTG+GGG 0.565833 5:+108918182 MsG0580030249.01.T01:five_prime_UTR
ACACTGAATTTGATGGGGCA+GGG 0.579075 5:-108919711 None:intergenic
AATGGGAGAGTCTACGGAGA+AGG 0.592110 5:+108918228 MsG0580030249.01.T01:exon
AATCTTGGAGCCTTATCTCA+AGG 0.607734 5:+108919598 MsG0580030249.01.T01:CDS
CGCTTCTCGATCTCCAACTG+CGG 0.610594 5:+108918438 MsG0580030249.01.T01:CDS
AAGCGCCAACAAGATTCCCA+TGG 0.623290 5:-108918389 None:intergenic
AACAAGATTCCCATGGCGTG+AGG 0.632713 5:-108918382 None:intergenic
ATGGCGTGAGGATATAGAGA+AGG 0.642651 5:-108918370 None:intergenic
CTTGGACACTGAATTTGATG+GGG 0.656354 5:-108919716 None:intergenic
AGTCTCAATCAAATTACCGA+AGG 0.656652 5:+108919832 MsG0580030249.01.T01:three_prime_UTR
TTGTTGGCGCTTAAACACAA+AGG 0.670200 5:+108918400 MsG0580030249.01.T01:CDS
TGAGAATAGAAACACACAAG+CGG 0.678956 5:+108918696 MsG0580030249.01.T01:CDS
TCAATCAAATTAATCGTTGG+GGG 0.692972 5:+108918183 MsG0580030249.01.T01:five_prime_UTR
ATGCCAAGTACACAAGCCAG+AGG 0.700436 5:-108919867 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTGCTTTAATAAATATTTA+TGG - Chr5:108918108-108918127 None:intergenic 15.0%
!! TGAATTAAACAATAAATTCA+AGG - Chr5:108918928-108918947 None:intergenic 15.0%
!! TTGTTTATATCATTTCATAT+AGG + Chr5:108919531-108919550 MsG0580030249.01.T01:intron 15.0%
!!! AAAGCTTTTCTTAATAAATT+GGG + Chr5:108918469-108918488 MsG0580030249.01.T01:CDS 15.0%
!!! ATCCAAATTTTTTTTTTACT+TGG - Chr5:108919737-108919756 None:intergenic 15.0%
!!! ATTTTTTACTAAAATCACAT+TGG - Chr5:108918787-108918806 None:intergenic 15.0%
!!! TAAAGCTTTTCTTAATAAAT+TGG + Chr5:108918468-108918487 MsG0580030249.01.T01:CDS 15.0%
!! AAGGCTAAAAATTATTATAG+AGG - Chr5:108918909-108918928 None:intergenic 20.0%
!! GTCCAAGTAAAAAAAAAATT+TGG + Chr5:108919732-108919751 MsG0580030249.01.T01:exon 20.0%
!! TGAACAAAACAACAAAAATT+AGG - Chr5:108918580-108918599 None:intergenic 20.0%
!!! AATCATTTTATAGCTTGTTT+TGG + Chr5:108919010-108919029 MsG0580030249.01.T01:intron 20.0%
!!! TAATTTTTGTTGTTTTGTTC+AGG + Chr5:108918579-108918598 MsG0580030249.01.T01:intron 20.0%
!!! TATATCATATGCATCATTTT+TGG - Chr5:108919445-108919464 None:intergenic 20.0%
!!! TTTAGCTTGATATTAGAATT+TGG + Chr5:108918875-108918894 MsG0580030249.01.T01:intron 20.0%
!! TAACAATGATAGTGTATGTT+TGG + Chr5:108918732-108918751 MsG0580030249.01.T01:intron 25.0%
!! TTGAACAAAAGTTTCACTTT+TGG + Chr5:108918825-108918844 MsG0580030249.01.T01:intron 25.0%
!! TTTCATATAGGTCTCTAAAA+TGG + Chr5:108919543-108919562 MsG0580030249.01.T01:intron 25.0%
!!! TTCATATAGGTCTCTAAAAT+GGG + Chr5:108919544-108919563 MsG0580030249.01.T01:intron 25.0%
AAGTGAGTTTGACAAAATCA+TGG - Chr5:108919417-108919436 None:intergenic 30.0%
AGTCACTTAGTTGGAAAAAT+GGG - Chr5:108919501-108919520 None:intergenic 30.0%
ATAGTGTATGTTTGGATTGA+TGG + Chr5:108918740-108918759 MsG0580030249.01.T01:intron 30.0%
ATTGAGACTACAATTCAAGT+AGG - Chr5:108919821-108919840 None:intergenic 30.0%
CTCAATCAAATTAATCGTTG+GGG + Chr5:108918182-108918201 MsG0580030249.01.T01:five_prime_UTR 30.0%
GCTCAATCAAATTAATCGTT+GGG + Chr5:108918181-108918200 MsG0580030249.01.T01:five_prime_UTR 30.0%
GTAAGTTGTTTATCCAAACA+CGG + Chr5:108919339-108919358 MsG0580030249.01.T01:intron 30.0%
TCAATCAAATTAATCGTTGG+GGG + Chr5:108918183-108918202 MsG0580030249.01.T01:five_prime_UTR 30.0%
TTCAGGAAATATATTCGAGT+TGG + Chr5:108918596-108918615 MsG0580030249.01.T01:intron 30.0%
TTGATGTATGTTTGGTTAAG+TGG + Chr5:108919039-108919058 MsG0580030249.01.T01:intron 30.0%
! ATCTGGCTTCTTAGAATAAT+CGG - Chr5:108919683-108919702 None:intergenic 30.0%
! GTTTACAGTAGAGTCAATTT+TGG + Chr5:108919119-108919138 MsG0580030249.01.T01:intron 30.0%
! TAGTCTAAAAAGTGAGTTGA+AGG - Chr5:108918990-108919009 None:intergenic 30.0%
!!! ACTAGCATTTTGGTTGATAA+TGG + Chr5:108919571-108919590 MsG0580030249.01.T01:CDS 30.0%
!!! CACACTTCTAGAATCAATTT+TGG + Chr5:108919192-108919211 MsG0580030249.01.T01:intron 30.0%
!!! CTAGCATTTTGGTTGATAAT+GGG + Chr5:108919572-108919591 MsG0580030249.01.T01:CDS 30.0%
!!! TAGCATTTTGGTTGATAATG+GGG + Chr5:108919573-108919592 MsG0580030249.01.T01:CDS 30.0%
ACTTGGACACTGAATTTGAT+GGG - Chr5:108919720-108919739 None:intergenic 35.0%
AGTCTCAATCAAATTACCGA+AGG + Chr5:108919832-108919851 MsG0580030249.01.T01:three_prime_UTR 35.0%
ATTATGAACAAGACCGTGTT+TGG - Chr5:108919355-108919374 None:intergenic 35.0%
CACAAAAGAACAAGCAGAAA+TGG + Chr5:108919653-108919672 MsG0580030249.01.T01:CDS 35.0%
CAGAGAATTCATTCAACTAC+TGG + Chr5:108918640-108918659 MsG0580030249.01.T01:CDS 35.0%
CAGTCACTTAGTTGGAAAAA+TGG - Chr5:108919502-108919521 None:intergenic 35.0%
CGCTCAATCAAATTAATCGT+TGG + Chr5:108918180-108918199 MsG0580030249.01.T01:five_prime_UTR 35.0%
GTTATGATCATCGATGTTGA+TGG - Chr5:108918132-108918151 None:intergenic 35.0%
TACTTGGACACTGAATTTGA+TGG - Chr5:108919721-108919740 None:intergenic 35.0%
TCCAACAGATAAGCTCAAAT+GGG - Chr5:108919235-108919254 None:intergenic 35.0%
TGAGAATAGAAACACACAAG+CGG + Chr5:108918696-108918715 MsG0580030249.01.T01:CDS 35.0%
TGAGTTTGACAAAATCATGG+TGG - Chr5:108919414-108919433 None:intergenic 35.0%
TTCCAACAGATAAGCTCAAA+TGG - Chr5:108919236-108919255 None:intergenic 35.0%
! AGATCGAAAACAACGAGTTT+TGG - Chr5:108918298-108918317 None:intergenic 35.0%
! TTACACAGTTTTCTCGAAAC+TGG + Chr5:108919623-108919642 MsG0580030249.01.T01:CDS 35.0%
!! AAAAAGAGCATCGAGCTAAA+TGG - Chr5:108918670-108918689 None:intergenic 35.0%
!! GGATTGACTTGATGTATGTT+TGG + Chr5:108919031-108919050 MsG0580030249.01.T01:intron 35.0%
!! GTTGATAATGGGGTAAATCT+TGG + Chr5:108919583-108919602 MsG0580030249.01.T01:CDS 35.0%
!! TTCGAGTTGGACTCATAAAA+CGG + Chr5:108918609-108918628 MsG0580030249.01.T01:CDS 35.0%
!!! AATTTTGGCTCCTCCAAAAA+TGG + Chr5:108919207-108919226 MsG0580030249.01.T01:intron 35.0%
ACCCATTTGAGCTTATCTGT+TGG + Chr5:108919231-108919250 MsG0580030249.01.T01:intron 40.0%
ATCAAATTAATCGTTGGGGG+AGG + Chr5:108918186-108918205 MsG0580030249.01.T01:five_prime_UTR 40.0%
CTGTGTAAGACCTTGAGATA+AGG - Chr5:108919611-108919630 None:intergenic 40.0%
CTTGGACACTGAATTTGATG+GGG - Chr5:108919719-108919738 None:intergenic 40.0%
TCAAATTAATCGTTGGGGGA+GGG + Chr5:108918187-108918206 MsG0580030249.01.T01:five_prime_UTR 40.0%
TTATGCAGCAGTCACTTAGT+TGG - Chr5:108919510-108919529 None:intergenic 40.0%
TTGTTGGCGCTTAAACACAA+AGG + Chr5:108918400-108918419 MsG0580030249.01.T01:CDS 40.0%
! AATCTTGGAGCCTTATCTCA+AGG + Chr5:108919598-108919617 MsG0580030249.01.T01:CDS 40.0%
! AATGGGAGTGACTAGCATTT+TGG + Chr5:108919561-108919580 MsG0580030249.01.T01:CDS 40.0%
!! CAAATGGGTTCCATTTTTGG+AGG - Chr5:108919220-108919239 None:intergenic 40.0%
!! GCTCAAATGGGTTCCATTTT+TGG - Chr5:108919223-108919242 None:intergenic 40.0%
AAGCGAAATGGGAGAGTCTA+CGG + Chr5:108918222-108918241 MsG0580030249.01.T01:exon 45.0%
AAGGCCAAACATGTTACCTC+TGG + Chr5:108919851-108919870 MsG0580030249.01.T01:three_prime_UTR 45.0%
ACACTGAATTTGATGGGGCA+GGG - Chr5:108919714-108919733 None:intergenic 45.0%
AGAGCAGAGAGAAAGCGAAA+TGG + Chr5:108918210-108918229 MsG0580030249.01.T01:exon 45.0%
ATGGCGTGAGGATATAGAGA+AGG - Chr5:108918373-108918392 None:intergenic 45.0%
CAAGCCAGAGGTAACATGTT+TGG - Chr5:108919858-108919877 None:intergenic 45.0%
GAGCAGAGAGAAAGCGAAAT+GGG + Chr5:108918211-108918230 MsG0580030249.01.T01:exon 45.0%
GCTTTAGCAATATCCGCAGT+TGG - Chr5:108918454-108918473 None:intergenic 45.0%
TCGATCTCGATTACACTCTC+TGG + Chr5:108918311-108918330 MsG0580030249.01.T01:CDS 45.0%
TCTCTATATCCTCACGCCAT+GGG + Chr5:108918373-108918392 MsG0580030249.01.T01:CDS 45.0%
TGCTTCGTTCTTTGTTGCTC+AGG + Chr5:108918495-108918514 MsG0580030249.01.T01:CDS 45.0%
TTCTCTATATCCTCACGCCA+TGG + Chr5:108918372-108918391 MsG0580030249.01.T01:CDS 45.0%
! GAGGTAACATGTTTGGCCTT+CGG - Chr5:108919851-108919870 None:intergenic 45.0%
! TTACCTCTGGCTTGTGTACT+TGG + Chr5:108919864-108919883 MsG0580030249.01.T01:three_prime_UTR 45.0%
AACAAGATTCCCATGGCGTG+AGG - Chr5:108918385-108918404 None:intergenic 50.0%
AAGCGCCAACAAGATTCCCA+TGG - Chr5:108918392-108918411 None:intergenic 50.0%
AATGGGAGAGTCTACGGAGA+AGG + Chr5:108918228-108918247 MsG0580030249.01.T01:exon 50.0%
ATGCCAAGTACACAAGCCAG+AGG - Chr5:108919870-108919889 None:intergenic 50.0%
GACACTGAATTTGATGGGGC+AGG - Chr5:108919715-108919734 None:intergenic 50.0%
GATCGACACTCGCAGTAGAA+AGG - Chr5:108918337-108918356 None:intergenic 50.0%
TAGAGAAGGAGTTTCGCGCT+TGG - Chr5:108918359-108918378 None:intergenic 50.0%
TCACGCCATGGGAATCTTGT+TGG + Chr5:108918384-108918403 MsG0580030249.01.T01:CDS 50.0%
TGAATTTGATGGGGCAGGGT+TGG - Chr5:108919710-108919729 None:intergenic 50.0%
CGCTTCTCGATCTCCAACTG+CGG + Chr5:108918438-108918457 MsG0580030249.01.T01:CDS 55.0%
!! GGGGCAGGGTTGGAAGTATC+TGG - Chr5:108919700-108919719 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 108918099 108919926 108918099 ID=MsG0580030249.01;Name=MsG0580030249.01
Chr5 mRNA 108918099 108919926 108918099 ID=MsG0580030249.01.T01;Parent=MsG0580030249.01;Name=MsG0580030249.01.T01;_AED=0.29;_eAED=0.29;_QI=130|1|1|1|1|1|3|185|197
Chr5 exon 108918099 108918516 108918099 ID=MsG0580030249.01.T01:exon:15401;Parent=MsG0580030249.01.T01
Chr5 exon 108918601 108918717 108918601 ID=MsG0580030249.01.T01:exon:15402;Parent=MsG0580030249.01.T01
Chr5 exon 108919553 108919926 108919553 ID=MsG0580030249.01.T01:exon:15403;Parent=MsG0580030249.01.T01
Chr5 five_prime_UTR 108918099 108918228 108918099 ID=MsG0580030249.01.T01:five_prime_utr;Parent=MsG0580030249.01.T01
Chr5 CDS 108918229 108918516 108918229 ID=MsG0580030249.01.T01:cds;Parent=MsG0580030249.01.T01
Chr5 CDS 108918601 108918717 108918601 ID=MsG0580030249.01.T01:cds;Parent=MsG0580030249.01.T01
Chr5 CDS 108919553 108919741 108919553 ID=MsG0580030249.01.T01:cds;Parent=MsG0580030249.01.T01
Chr5 three_prime_UTR 108919742 108919926 108919742 ID=MsG0580030249.01.T01:three_prime_utr;Parent=MsG0580030249.01.T01
Gene Sequence

>MsG0580030249.01.T01

ATGGGAGAGTCTACGGAGAAGGCGAAGTCAGAAGCAGTGAAGATAATAGAATCATTCGAAGTTCTTCCAAAACTCGTTGTTTTCGATCTCGATTACACTCTCTGGCCTTTCTACTGCGAGTGTCGATCCAAGCGCGAAACTCCTTCTCTATATCCTCACGCCATGGGAATCTTGTTGGCGCTTAAACACAAAGGAATTGATATTGCAATCGCTTCTCGATCTCCAACTGCGGATATTGCTAAAGCTTTTCTTAATAAATTGGGAATTGCTTCGTTCTTTGTTGCTCAGGAAATATATTCGAGTTGGACTCATAAAACGGATCATTTTCAGAGAATTCATTCAACTACTGGAATTCCATTTAGCTCGATGCTCTTTTTTGATGATGAGAATAGAAACACACAAGCGGTCTCTAAAATGGGAGTGACTAGCATTTTGGTTGATAATGGGGTAAATCTTGGAGCCTTATCTCAAGGTCTTACACAGTTTTCTCGAAACTGGAATACATCACAAAAGAACAAGCAGAAATGGCTATCCGATTATTCTAAGAAGCCAGATACTTCCAACCCTGCCCCATCAAATTCAGTGTCCAAGTAA

Protein sequence

>MsG0580030249.01.T01

MGESTEKAKSEAVKIIESFEVLPKLVVFDLDYTLWPFYCECRSKRETPSLYPHAMGILLALKHKGIDIAIASRSPTADIAKAFLNKLGIASFFVAQEIYSSWTHKTDHFQRIHSTTGIPFSSMLFFDDENRNTQAVSKMGVTSILVDNGVNLGALSQGLTQFSRNWNTSQKNKQKWLSDYSKKPDTSNPAPSNSVSK*