AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008758.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008758.01.T01 MTR_2g049790 92.647 68 5 0 1 68 1 68 2.00e-38 132
MsG0280008758.01.T01 MTR_5g088350 67.308 52 17 0 17 68 7 58 3.63e-19 79.0
MsG0280008758.01.T01 MTR_5g088350 67.308 52 17 0 17 68 7 58 6.99e-19 79.0
MsG0280008758.01.T01 MTR_4g114670 63.462 52 19 0 17 68 5 56 3.29e-18 76.6
MsG0280008758.01.T01 MTR_4g114670 63.462 52 19 0 17 68 5 56 3.59e-18 77.0
MsG0280008758.01.T01 MTR_5g088350 67.308 52 17 0 17 68 7 58 3.64e-18 76.6
MsG0280008758.01.T01 MTR_4g114670 63.462 52 19 0 17 68 5 56 3.88e-18 76.6
MsG0280008758.01.T01 MTR_8g024590 58.929 56 23 0 13 68 7 62 4.99e-18 76.3
MsG0280008758.01.T01 MTR_5g067150 66.000 50 17 0 19 68 5 54 5.30e-18 76.3
MsG0280008758.01.T01 MTR_5g067150 66.000 50 17 0 19 68 5 54 9.03e-18 75.5
MsG0280008758.01.T01 MTR_5g067150 66.000 50 17 0 19 68 5 54 1.32e-17 75.1
MsG0280008758.01.T01 MTR_8g024600 61.538 52 20 0 17 68 11 62 1.69e-17 75.1
MsG0280008758.01.T01 MTR_1g103760 54.717 53 24 0 16 68 5 57 1.00e-12 61.6
MsG0280008758.01.T01 MTR_4g128820 54.000 50 23 0 21 70 8 57 2.28e-12 60.5
MsG0280008758.01.T01 MTR_3g072320 50.000 52 26 0 16 67 5 56 2.15e-11 57.8
MsG0280008758.01.T01 MTR_2g045470 58.696 46 19 0 15 60 12 57 2.48e-11 57.4
MsG0280008758.01.T01 MTR_4g131060 56.250 48 21 0 21 68 16 63 3.15e-11 57.0
MsG0280008758.01.T01 MTR_7g072575 50.943 53 26 0 16 68 5 57 6.77e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008758.01.T01 AT1G30270 77.551 49 11 0 20 68 28 76 9.73e-21 84.3
MsG0280008758.01.T01 AT1G30270 77.551 49 11 0 20 68 28 76 1.07e-20 84.0
MsG0280008758.01.T01 AT2G26980 69.231 52 16 0 17 68 8 59 3.57e-20 80.9
MsG0280008758.01.T01 AT2G26980 69.231 52 16 0 17 68 8 59 2.20e-19 80.1
MsG0280008758.01.T01 AT2G26980 69.231 52 16 0 17 68 8 59 2.48e-19 80.1
MsG0280008758.01.T01 AT2G26980 69.231 52 16 0 17 68 8 59 6.59e-19 79.0
MsG0280008758.01.T01 AT2G26980 69.231 52 16 0 17 68 18 69 6.84e-19 79.0
MsG0280008758.01.T01 AT1G01140 53.968 63 29 0 6 68 2 64 8.69e-19 78.6
MsG0280008758.01.T01 AT1G01140 53.968 63 29 0 6 68 2 64 8.93e-19 78.6
MsG0280008758.01.T01 AT1G01140 53.968 63 29 0 6 68 2 64 9.07e-19 78.6
MsG0280008758.01.T01 AT4G24400 64.000 50 18 0 19 68 5 54 5.83e-17 73.6
MsG0280008758.01.T01 AT5G35410 64.000 50 18 0 19 68 7 56 7.01e-17 73.2
MsG0280008758.01.T01 AT5G35410 64.000 50 18 0 19 68 7 56 7.61e-17 73.2
MsG0280008758.01.T01 AT4G24400 64.000 50 18 0 19 68 5 54 8.74e-17 72.8
MsG0280008758.01.T01 AT5G21326 64.000 50 18 0 19 68 9 58 1.96e-16 72.0
MsG0280008758.01.T01 AT5G21222 57.692 52 22 0 17 68 7 58 1.03e-13 64.3
MsG0280008758.01.T01 AT5G21222 57.692 52 22 0 17 68 7 58 1.03e-13 64.3
MsG0280008758.01.T01 AT5G21222 57.692 52 22 0 17 68 7 58 1.03e-13 64.3
MsG0280008758.01.T01 AT5G21222 57.692 52 22 0 17 68 7 58 1.03e-13 64.3
MsG0280008758.01.T01 AT5G01810 59.574 47 19 0 22 68 11 57 6.02e-13 62.0
MsG0280008758.01.T01 AT5G01810 59.574 47 19 0 22 68 11 57 6.02e-13 62.0
MsG0280008758.01.T01 AT5G01810 59.574 47 19 0 22 68 11 57 6.25e-13 62.0
MsG0280008758.01.T01 AT1G29230 54.167 48 22 0 21 68 72 119 8.88e-13 61.6
MsG0280008758.01.T01 AT5G45810 56.250 48 21 0 21 68 26 73 2.07e-12 60.5
MsG0280008758.01.T01 AT4G18700 54.167 48 22 0 21 68 24 71 6.82e-12 59.3
MsG0280008758.01.T01 AT5G45820 57.447 47 20 0 22 68 11 57 8.81e-12 58.9
MsG0280008758.01.T01 AT4G14580 48.333 60 27 1 16 71 14 73 1.09e-11 58.5
MsG0280008758.01.T01 AT5G57630 50.943 53 26 0 16 68 5 57 1.21e-11 58.5
MsG0280008758.01.T01 AT5G57630 50.943 53 26 0 16 68 5 57 1.41e-11 58.2
MsG0280008758.01.T01 AT3G23000 35.616 73 43 1 3 71 5 77 1.69e-11 58.2
MsG0280008758.01.T01 AT3G01090 46.970 66 35 0 3 68 22 87 2.30e-11 57.8
MsG0280008758.01.T01 AT3G17510 63.415 41 15 0 19 59 16 56 3.47e-11 57.0
MsG0280008758.01.T01 AT2G34180 60.000 40 16 0 22 61 56 95 6.40e-11 56.2
MsG0280008758.01.T01 AT3G29160 48.387 62 32 0 7 68 4 65 8.18e-11 56.2
MsG0280008758.01.T01 AT3G29160 48.387 62 32 0 7 68 4 65 8.18e-11 56.2

Find 15 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGCATAAAATGATTCGTC+AGG 0.448170 2:-37758167 MsG0280008758.01.T01:CDS
CAACGGCGGACGTGCGAAGG+CGG 0.465088 2:-37758308 MsG0280008758.01.T01:CDS
CAGCAACGGCGGACGTGCGA+AGG 0.469154 2:-37758311 MsG0280008758.01.T01:CDS
TATGAGCTGGGTAGGACGTT+AGG 0.472521 2:-37758280 MsG0280008758.01.T01:CDS
TTTGCGAGACATGTTGAAAC+TGG 0.475203 2:-37758232 MsG0280008758.01.T01:CDS
AGCAACGGAGGCAGCAGCAA+CGG 0.481688 2:-37758325 MsG0280008758.01.T01:CDS
AACGGCGGACGTGCGAAGGC+GGG 0.484040 2:-37758307 MsG0280008758.01.T01:CDS
CGGGAAAGTATGAGCTGGGT+AGG 0.549445 2:-37758288 MsG0280008758.01.T01:CDS
GAAGGCGGGAAAGTATGAGC+TGG 0.568172 2:-37758293 MsG0280008758.01.T01:CDS
AACGGAGGCAGCAGCAACGG+CGG 0.578341 2:-37758322 MsG0280008758.01.T01:CDS
AAGGCGGGAAAGTATGAGCT+GGG 0.606003 2:-37758292 MsG0280008758.01.T01:CDS
CTGGGTAGGACGTTAGGTGA+AGG 0.611905 2:-37758274 MsG0280008758.01.T01:CDS
AGGTGAAGGTAATTTCGCGA+AGG 0.640213 2:-37758260 MsG0280008758.01.T01:CDS
TCACGTTCATCGAATAGCAA+CGG 0.671573 2:-37758340 MsG0280008758.01.T01:CDS
CGTTCATCGAATAGCAACGG+AGG 0.695102 2:-37758337 MsG0280008758.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CAAGCATAAAATGATTCGTC+AGG - Chr2:37758329-37758348 MsG0280008758.01.T01:CDS 35.0%
TCACGTTCATCGAATAGCAA+CGG - Chr2:37758156-37758175 MsG0280008758.01.T01:CDS 40.0%
TTTGCGAGACATGTTGAAAC+TGG - Chr2:37758264-37758283 MsG0280008758.01.T01:CDS 40.0%
AGGTGAAGGTAATTTCGCGA+AGG - Chr2:37758236-37758255 MsG0280008758.01.T01:CDS 45.0%
CGTTCATCGAATAGCAACGG+AGG - Chr2:37758159-37758178 MsG0280008758.01.T01:CDS 50.0%
TATGAGCTGGGTAGGACGTT+AGG - Chr2:37758216-37758235 MsG0280008758.01.T01:CDS 50.0%
! AAGGCGGGAAAGTATGAGCT+GGG - Chr2:37758204-37758223 MsG0280008758.01.T01:CDS 50.0%
CTGGGTAGGACGTTAGGTGA+AGG - Chr2:37758222-37758241 MsG0280008758.01.T01:CDS 55.0%
! CGGGAAAGTATGAGCTGGGT+AGG - Chr2:37758208-37758227 MsG0280008758.01.T01:CDS 55.0%
! GAAGGCGGGAAAGTATGAGC+TGG - Chr2:37758203-37758222 MsG0280008758.01.T01:CDS 55.0%
AGCAACGGAGGCAGCAGCAA+CGG - Chr2:37758171-37758190 MsG0280008758.01.T01:CDS 60.0%
AACGGAGGCAGCAGCAACGG+CGG - Chr2:37758174-37758193 MsG0280008758.01.T01:CDS 65.0%
AACGGCGGACGTGCGAAGGC+GGG - Chr2:37758189-37758208 MsG0280008758.01.T01:CDS 70.0%
CAACGGCGGACGTGCGAAGG+CGG - Chr2:37758188-37758207 MsG0280008758.01.T01:CDS 70.0%
CAGCAACGGCGGACGTGCGA+AGG - Chr2:37758185-37758204 MsG0280008758.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 37758150 37758368 37758150 ID=MsG0280008758.01;Name=MsG0280008758.01
Chr2 mRNA 37758150 37758368 37758150 ID=MsG0280008758.01.T01;Parent=MsG0280008758.01;Name=MsG0280008758.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|72
Chr2 exon 37758150 37758368 37758150 ID=MsG0280008758.01.T01:exon:9764;Parent=MsG0280008758.01.T01
Chr2 CDS 37758150 37758368 37758150 ID=MsG0280008758.01.T01:cds;Parent=MsG0280008758.01.T01
Gene Sequence

>MsG0280008758.01.T01

ATGACGTCACGTTCATCGAATAGCAACGGAGGCAGCAGCAACGGCGGACGTGCGAAGGCGGGAAAGTATGAGCTGGGTAGGACGTTAGGTGAAGGTAATTTCGCGAAGGTGAAGTTTGCGAGACATGTTGAAACTGGTGATCATGTTGCTATCAAGATACTTGATAAAGAAAAAATTCTCAAGCATAAAATGATTCGTCAGGTTAATTCTTTGTTTTAA

Protein sequence

>MsG0280008758.01.T01

MTSRSSNSNGGSSNGGRAKAGKYELGRTLGEGNFAKVKFARHVETGDHVAIKILDKEKILKHKMIRQVNSLF*