AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011343.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011343.01.T01 MTR_2g102490 81.548 168 15 4 2 166 14 168 2.46e-82 241
MsG0280011343.01.T01 MTR_1g080860 40.777 206 78 7 4 166 17 221 2.19e-38 131
MsG0280011343.01.T01 MTR_1g080860 40.777 206 78 7 4 166 17 221 2.48e-38 131
MsG0280011343.01.T01 MTR_6g488150 36.842 209 83 8 4 166 17 222 2.04e-35 124
MsG0280011343.01.T01 MTR_1g093240 41.000 200 76 8 4 166 10 204 4.01e-35 122
MsG0280011343.01.T01 MTR_1g070520 41.748 206 72 11 3 166 14 213 5.84e-35 122
MsG0280011343.01.T01 MTR_1g093240 41.000 200 76 8 4 166 10 204 6.96e-35 122
MsG0280011343.01.T01 MTR_1g093240 41.000 200 76 8 4 166 10 204 1.40e-34 121
MsG0280011343.01.T01 MTR_7g096090 39.810 211 74 9 4 166 13 218 1.01e-33 119
MsG0280011343.01.T01 MTR_1g076270 41.860 172 65 7 4 166 13 158 4.03e-31 110
MsG0280011343.01.T01 MTR_1g093350 41.860 172 65 7 4 166 13 158 4.03e-31 110
MsG0280011343.01.T01 MTR_1g040675 38.728 173 77 6 7 166 6 162 4.21e-31 110
MsG0280011343.01.T01 MTR_8g103030 41.892 148 59 4 43 166 117 261 1.23e-30 112
MsG0280011343.01.T01 MTR_1g040675 38.953 172 76 6 7 165 6 161 1.36e-30 109
MsG0280011343.01.T01 MTR_1g070830 37.778 180 83 5 4 166 14 181 4.57e-30 108
MsG0280011343.01.T01 MTR_5g030710 40.972 144 57 4 48 166 163 303 4.74e-29 109
MsG0280011343.01.T01 MTR_5g067350 44.526 137 51 5 52 166 182 315 6.42e-29 109
MsG0280011343.01.T01 MTR_2g101500 40.000 160 58 5 31 165 147 293 4.06e-28 106
MsG0280011343.01.T01 MTR_8g067530 42.520 127 46 4 63 165 200 323 3.18e-26 102
MsG0280011343.01.T01 MTR_6g488150 35.404 161 63 6 4 126 17 174 6.85e-23 90.1
MsG0280011343.01.T01 MTR_4g011880 36.747 166 75 5 4 166 8 146 7.47e-22 87.0
MsG0280011343.01.T01 MTR_8g014520 32.716 162 70 5 41 165 84 243 5.11e-16 73.6
MsG0280011343.01.T01 MTR_8g014520 32.716 162 70 5 41 165 84 243 5.87e-16 73.2
MsG0280011343.01.T01 MTR_2g100780 29.814 161 66 3 52 165 77 237 3.72e-15 71.2
MsG0280011343.01.T01 MTR_2g100780 29.814 161 66 3 52 165 77 237 6.00e-15 70.5
MsG0280011343.01.T01 MTR_4g124300 31.967 122 66 4 50 166 58 167 7.20e-15 68.9
MsG0280011343.01.T01 MTR_5g067350 43.158 95 34 4 48 126 180 270 9.58e-15 70.1
MsG0280011343.01.T01 MTR_1g069495 40.449 89 44 2 86 165 147 235 1.06e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011343.01.T01 AT1G04250 44.211 190 76 7 4 165 9 196 1.79e-42 141
MsG0280011343.01.T01 AT4G14550 42.424 198 73 8 4 165 3 195 4.94e-40 134
MsG0280011343.01.T01 AT4G14550 42.424 198 73 8 4 165 3 195 1.16e-39 134
MsG0280011343.01.T01 AT4G14550 42.424 198 73 8 4 165 3 195 1.21e-39 134
MsG0280011343.01.T01 AT3G04730 40.488 205 76 7 4 166 4 204 9.30e-37 127
MsG0280011343.01.T01 AT3G23050 41.063 207 74 9 4 166 8 210 1.32e-35 123
MsG0280011343.01.T01 AT3G23050 41.063 207 74 9 4 166 8 210 2.31e-35 124
MsG0280011343.01.T01 AT1G04240 38.728 173 82 5 4 166 7 165 2.60e-31 111
MsG0280011343.01.T01 AT3G23030 40.123 162 74 6 4 165 11 149 3.12e-31 111
MsG0280011343.01.T01 AT3G23030 40.123 162 74 6 4 165 98 236 2.63e-30 111
MsG0280011343.01.T01 AT1G04240 38.728 173 82 5 4 166 107 265 4.16e-30 111
MsG0280011343.01.T01 AT4G29080 33.333 237 81 7 4 166 40 273 1.04e-28 107
MsG0280011343.01.T01 AT5G43700 35.673 171 80 6 4 166 13 161 2.57e-28 103
MsG0280011343.01.T01 AT5G65670 45.238 126 46 5 63 166 180 304 5.36e-28 106
MsG0280011343.01.T01 AT5G65670 45.238 126 46 5 63 166 180 304 5.47e-28 106
MsG0280011343.01.T01 AT2G22670 37.222 180 77 6 12 166 119 287 3.08e-27 104
MsG0280011343.01.T01 AT2G22670 37.222 180 77 6 12 166 119 287 3.08e-27 104
MsG0280011343.01.T01 AT2G22670 37.222 180 77 6 12 166 119 287 3.28e-27 104
MsG0280011343.01.T01 AT2G22670 37.222 180 77 6 12 166 119 287 3.28e-27 104
MsG0280011343.01.T01 AT2G22670 37.222 180 77 6 12 166 136 304 4.22e-27 104
MsG0280011343.01.T01 AT1G15580 38.235 170 63 6 4 166 10 144 6.57e-27 99.8
MsG0280011343.01.T01 AT3G15540 35.795 176 83 7 4 165 8 167 2.34e-26 99.4
MsG0280011343.01.T01 AT1G52830 35.119 168 93 4 4 166 8 164 3.30e-26 98.6
MsG0280011343.01.T01 AT4G14560 38.650 163 74 6 4 165 9 146 8.18e-26 97.1
MsG0280011343.01.T01 AT4G14550 40.268 149 54 5 4 121 3 147 4.00e-23 90.5
MsG0280011343.01.T01 AT1G80390 44.538 119 44 5 7 119 19 121 3.72e-21 84.0
MsG0280011343.01.T01 AT3G16500 30.244 205 96 6 5 166 36 236 1.89e-19 82.8
MsG0280011343.01.T01 AT3G16500 30.244 205 96 6 5 166 36 236 2.20e-19 82.4
MsG0280011343.01.T01 AT3G23050 38.125 160 51 7 4 121 8 161 2.24e-19 80.5
MsG0280011343.01.T01 AT3G17600 32.593 135 62 4 45 165 21 140 4.16e-17 74.3
MsG0280011343.01.T01 AT5G25890 31.944 144 64 3 37 166 24 147 1.39e-15 70.9
MsG0280011343.01.T01 AT2G33310 35.385 130 73 5 45 166 85 211 7.72e-15 70.1
MsG0280011343.01.T01 AT2G33310 35.385 130 73 5 45 166 84 210 7.87e-15 70.1
MsG0280011343.01.T01 AT2G33310 35.385 130 73 5 45 166 84 210 7.87e-15 70.1
MsG0280011343.01.T01 AT1G04550 28.090 178 72 7 30 166 41 203 1.26e-13 66.6
MsG0280011343.01.T01 AT4G28640 33.083 133 56 3 66 165 88 220 3.47e-13 65.5
MsG0280011343.01.T01 AT4G28640 33.083 133 56 3 66 165 88 220 3.53e-13 65.5
MsG0280011343.01.T01 AT1G04100 40.000 90 40 3 87 166 154 239 5.92e-13 65.1
MsG0280011343.01.T01 AT4G28640 33.083 133 56 3 66 165 88 220 1.22e-12 64.7
MsG0280011343.01.T01 AT2G46990 39.024 82 39 2 86 166 86 157 1.74e-11 59.3
MsG0280011343.01.T01 AT2G46990 39.024 82 39 2 86 166 86 157 2.14e-11 59.7
MsG0280011343.01.T01 AT3G62100 39.506 81 40 2 86 166 84 155 5.79e-11 58.2
MsG0280011343.01.T01 AT3G62100 39.506 81 40 2 86 166 84 155 7.65e-11 58.2

Find 36 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGAATGAGAAGAAATTCA+TGG 0.319723 2:+82682400 MsG0280011343.01.T01:CDS
TAATAAGATGAATATTATTC+AGG 0.355657 2:+82681742 None:intergenic
GGTTCTTACTTTGATGTTGT+TGG 0.366642 2:-82681926 None:intergenic
GTTCTTACTTTGATGTTGTT+GGG 0.367350 2:-82681925 None:intergenic
ATATATATGAACTTACTTCC+AGG 0.371837 2:-82682501 None:intergenic
GAGCTTACCCTTGGTCTTCC+CGG 0.380531 2:+82681770 MsG0280011343.01.T01:CDS
TACGTGAAAGTTGCGGTTGA+TGG 0.380805 2:+82682118 MsG0280011343.01.T01:CDS
ATGGTGACTGGATGTTGCTT+GGG 0.460851 2:+82682471 MsG0280011343.01.T01:CDS
ACATGTGGTGGGATGGCCAC+CGG 0.463192 2:+82682057 MsG0280011343.01.T01:CDS
GGTGGGATGGCCACCGGTGA+GGG 0.473868 2:+82682063 MsG0280011343.01.T01:CDS
TGATGTTGTTGGGAATTTAG+TGG 0.488099 2:-82681915 None:intergenic
GCAAACATGGTATTCAATGC+AGG 0.501093 2:-82682199 None:intergenic
TGGTGGGATGGCCACCGGTG+AGG 0.504156 2:+82682062 MsG0280011343.01.T01:CDS
ATGAGAAGTACTAGAAGTTG+AGG 0.504166 2:-82681864 None:intergenic
GATGGTGACTGGATGTTGCT+TGG 0.516052 2:+82682470 MsG0280011343.01.T01:CDS
CAGGAGACTGAGCTTACCCT+TGG 0.519336 2:+82681761 MsG0280011343.01.T01:CDS
TGTTGCTTGGGGATGCACCC+TGG 0.523036 2:+82682483 MsG0280011343.01.T01:CDS
AGCTCCATATCTAAGAAAAG+TGG 0.534799 2:+82682141 MsG0280011343.01.T01:CDS
TGCATGCAGGGAACATGTGG+TGG 0.534930 2:+82682045 MsG0280011343.01.T01:intron
TATATATGAACTTACTTCCA+GGG 0.535639 2:-82682500 None:intergenic
GCAGGGAACATGTGGTGGGA+TGG 0.542566 2:+82682050 MsG0280011343.01.T01:intron
TTCCTACTTGCCCTCACCGG+TGG 0.558621 2:-82682073 None:intergenic
ACGAAGATAAAGATGGTGAC+TGG 0.565436 2:+82682459 MsG0280011343.01.T01:CDS
GCATGCAGGGAACATGTGGT+GGG 0.566681 2:+82682046 MsG0280011343.01.T01:intron
GGTAGTAGTACCAAAACTGC+AGG 0.586869 2:+82681791 MsG0280011343.01.T01:CDS
GCTACATACGAAGATAAAGA+TGG 0.600922 2:+82682452 MsG0280011343.01.T01:CDS
TGGCAATTAGATGAAGATCA+AGG 0.605901 2:-82681841 None:intergenic
GGGTGCATGCAGGGAACATG+TGG 0.617054 2:+82682042 MsG0280011343.01.T01:intron
TACTACTACCGGGAAGACCA+AGG 0.619025 2:-82681778 None:intergenic
TTGCAAGTACGTGAAAGTTG+CGG 0.621140 2:+82682111 MsG0280011343.01.T01:CDS
GGCCACCGGTGAGGGCAAGT+AGG 0.630880 2:+82682071 MsG0280011343.01.T01:CDS
GAAGCAATTAGTAGCAAACA+TGG 0.636180 2:-82682212 None:intergenic
CAAACATGGTATTCAATGCA+GGG 0.638217 2:-82682198 None:intergenic
AGAAGTACTAGAAGTTGAGG+TGG 0.639519 2:-82681861 None:intergenic
ACTACTACCGGGAAGACCAA+GGG 0.663756 2:-82681777 None:intergenic
TGGTGACTGGATGTTGCTTG+GGG 0.674509 2:+82682472 MsG0280011343.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAATTAATGTAAAGT+TGG + Chr2:82682021-82682040 MsG0280011343.01.T01:intron 10.0%
!! AAATTAATTAATGTAAAGTT+GGG + Chr2:82682022-82682041 MsG0280011343.01.T01:intron 10.0%
!! TATGAAACATAAAAATAACA+TGG - Chr2:82682291-82682310 None:intergenic 15.0%
!! AAAAAATAGGAATGAATACA+TGG + Chr2:82682430-82682449 MsG0280011343.01.T01:CDS 20.0%
! ACATATATGTTGTGTGTTAT+GGG + Chr2:82682321-82682340 MsG0280011343.01.T01:intron 25.0%
! CAACTATCATTAATTATTGC+AGG + Chr2:82682368-82682387 MsG0280011343.01.T01:intron 25.0%
! CTGCAATAATTAATGATAGT+TGG - Chr2:82682370-82682389 None:intergenic 25.0%
! TACATATATGTTGTGTGTTA+TGG + Chr2:82682320-82682339 MsG0280011343.01.T01:intron 25.0%
!! TCATGGATTCTAGAAAAAAT+AGG + Chr2:82682417-82682436 MsG0280011343.01.T01:CDS 25.0%
ACTAGCTAGCTATATATGTA+CGG - Chr2:82681950-82681969 None:intergenic 30.0%
GATGAATGAGAAGAAATTCA+TGG + Chr2:82682400-82682419 MsG0280011343.01.T01:CDS 30.0%
GTTCTTACTTTGATGTTGTT+GGG - Chr2:82681928-82681947 None:intergenic 30.0%
!! AGATCCACTTTTCTTAGATA+TGG - Chr2:82682148-82682167 None:intergenic 30.0%
AGCTCCATATCTAAGAAAAG+TGG + Chr2:82682141-82682160 MsG0280011343.01.T01:CDS 35.0%
AGGGCAAGTAGGAAAAATAT+TGG + Chr2:82682082-82682101 MsG0280011343.01.T01:CDS 35.0%
ATATAGCTAGCTAGTTAGCT+AGG + Chr2:82681955-82681974 MsG0280011343.01.T01:intron 35.0%
CAAACATGGTATTCAATGCA+GGG - Chr2:82682201-82682220 None:intergenic 35.0%
GAAGCAATTAGTAGCAAACA+TGG - Chr2:82682215-82682234 None:intergenic 35.0%
GCTACATACGAAGATAAAGA+TGG + Chr2:82682452-82682471 MsG0280011343.01.T01:CDS 35.0%
GGTTCTTACTTTGATGTTGT+TGG - Chr2:82681929-82681948 None:intergenic 35.0%
TGATGTTGTTGGGAATTTAG+TGG - Chr2:82681918-82681937 None:intergenic 35.0%
TGGCAATTAGATGAAGATCA+AGG - Chr2:82681844-82681863 None:intergenic 35.0%
!! ATGAGAAGTACTAGAAGTTG+AGG - Chr2:82681867-82681886 None:intergenic 35.0%
ACGAAGATAAAGATGGTGAC+TGG + Chr2:82682459-82682478 MsG0280011343.01.T01:CDS 40.0%
GCAAACATGGTATTCAATGC+AGG - Chr2:82682202-82682221 None:intergenic 40.0%
TTGCAAGTACGTGAAAGTTG+CGG + Chr2:82682111-82682130 MsG0280011343.01.T01:CDS 40.0%
! ACGTTTTATGCCTGCAGTTT+TGG - Chr2:82681804-82681823 None:intergenic 40.0%
!! AGAAGTACTAGAAGTTGAGG+TGG - Chr2:82681864-82681883 None:intergenic 40.0%
!! TGCAGTTTTGGTACTACTAC+CGG - Chr2:82681792-82681811 None:intergenic 40.0%
ATGTAAAGTTGGGTGCATGC+AGG + Chr2:82682032-82682051 MsG0280011343.01.T01:intron 45.0%
GGTAGTAGTACCAAAACTGC+AGG + Chr2:82681791-82681810 MsG0280011343.01.T01:CDS 45.0%
TACGTGAAAGTTGCGGTTGA+TGG + Chr2:82682118-82682137 MsG0280011343.01.T01:CDS 45.0%
TGTAAAGTTGGGTGCATGCA+GGG + Chr2:82682033-82682052 MsG0280011343.01.T01:intron 45.0%
! ATGGTGACTGGATGTTGCTT+GGG + Chr2:82682471-82682490 MsG0280011343.01.T01:CDS 45.0%
! TTTTTCCTACTTGCCCTCAC+CGG - Chr2:82682079-82682098 None:intergenic 45.0%
!! GCAGTTTTGGTACTACTACC+GGG - Chr2:82681791-82681810 None:intergenic 45.0%
ACTACTACCGGGAAGACCAA+GGG - Chr2:82681780-82681799 None:intergenic 50.0%
GATGGTGACTGGATGTTGCT+TGG + Chr2:82682470-82682489 MsG0280011343.01.T01:CDS 50.0%
TACTACTACCGGGAAGACCA+AGG - Chr2:82681781-82681800 None:intergenic 50.0%
! TGGTGACTGGATGTTGCTTG+GGG + Chr2:82682472-82682491 MsG0280011343.01.T01:CDS 50.0%
CAGGAGACTGAGCTTACCCT+TGG + Chr2:82681761-82681780 MsG0280011343.01.T01:CDS 55.0%
GCATGCAGGGAACATGTGGT+GGG + Chr2:82682046-82682065 MsG0280011343.01.T01:intron 55.0%
TGCATGCAGGGAACATGTGG+TGG + Chr2:82682045-82682064 MsG0280011343.01.T01:intron 55.0%
! GAGCTTACCCTTGGTCTTCC+CGG + Chr2:82681770-82681789 MsG0280011343.01.T01:CDS 55.0%
GGGTGCATGCAGGGAACATG+TGG + Chr2:82682042-82682061 MsG0280011343.01.T01:intron 60.0%
TTCCTACTTGCCCTCACCGG+TGG - Chr2:82682076-82682095 None:intergenic 60.0%
! ACATGTGGTGGGATGGCCAC+CGG + Chr2:82682057-82682076 MsG0280011343.01.T01:CDS 60.0%
! GCAGGGAACATGTGGTGGGA+TGG + Chr2:82682050-82682069 MsG0280011343.01.T01:intron 60.0%
! TGTTGCTTGGGGATGCACCC+TGG + Chr2:82682483-82682502 MsG0280011343.01.T01:CDS 60.0%
GGCCACCGGTGAGGGCAAGT+AGG + Chr2:82682071-82682090 MsG0280011343.01.T01:CDS 70.0%
GGTGGGATGGCCACCGGTGA+GGG + Chr2:82682063-82682082 MsG0280011343.01.T01:CDS 70.0%
TGGTGGGATGGCCACCGGTG+AGG + Chr2:82682062-82682081 MsG0280011343.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 82681749 82682511 82681749 ID=MsG0280011343.01;Name=MsG0280011343.01
Chr2 mRNA 82681749 82682511 82681749 ID=MsG0280011343.01.T01;Parent=MsG0280011343.01;Name=MsG0280011343.01.T01;_AED=0.50;_eAED=0.53;_QI=0|0|0|1|1|1|3|0|166
Chr2 exon 82681749 82681939 82681749 ID=MsG0280011343.01.T01:exon:12317;Parent=MsG0280011343.01.T01
Chr2 exon 82682054 82682241 82682054 ID=MsG0280011343.01.T01:exon:12318;Parent=MsG0280011343.01.T01
Chr2 exon 82682390 82682511 82682390 ID=MsG0280011343.01.T01:exon:12319;Parent=MsG0280011343.01.T01
Chr2 CDS 82681749 82681939 82681749 ID=MsG0280011343.01.T01:cds;Parent=MsG0280011343.01.T01
Chr2 CDS 82682054 82682241 82682054 ID=MsG0280011343.01.T01:cds;Parent=MsG0280011343.01.T01
Chr2 CDS 82682390 82682511 82682390 ID=MsG0280011343.01.T01:cds;Parent=MsG0280011343.01.T01
Gene Sequence

>MsG0280011343.01.T01

ATGAATATTATTCAGGAGACTGAGCTTACCCTTGGTCTTCCCGGTAGTAGTACCAAAACTGCAGGCATAAAACGTAGCTTCTCTCACACTCACCTTGATCTTCATCTAATTGCCACCTCAACTTCTAGTACTTCTCATCATCATAATTCACTAAATTCTAATAATACCACTAAATTCCCAACAACATCAAAGGAACATGTGGTGGGATGGCCACCGGTGAGGGCAAGTAGGAAAAATATTGGTAGTAGTTGCAAGTACGTGAAAGTTGCGGTTGATGGAGCTCCATATCTAAGAAAAGTGGATCTTGAAGTTTATGAAAGTTATGATCATCTCTTCCCTGCATTGAATACCATGTTTGCTACTAATTGCTTCAATATACGTAATGATTTGATGAATGAGAAGAAATTCATGGATTCTAGAAAAAATAGGAATGAATACATGGCTACATACGAAGATAAAGATGGTGACTGGATGTTGCTTGGGGATGCACCCTGGAAGTAA

Protein sequence

>MsG0280011343.01.T01

MNIIQETELTLGLPGSSTKTAGIKRSFSHTHLDLHLIATSTSSTSHHHNSLNSNNTTKFPTTSKEHVVGWPPVRASRKNIGSSCKYVKVAVDGAPYLRKVDLEVYESYDHLFPALNTMFATNCFNIRNDLMNEKKFMDSRKNRNEYMATYEDKDGDWMLLGDAPWK*