AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035905.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035905.01.T02 MTR_6g092790 98.020 101 1 1 2 101 57 157 2.69e-70 207
MsG0680035905.01.T02 MTR_8g101550 65.979 97 32 1 2 97 49 145 8.61e-45 142
MsG0680035905.01.T02 MTR_6g091720 51.724 58 20 1 7 64 52 101 3.80e-15 66.6
MsG0680035905.01.T02 MTR_6g091750 48.276 58 22 1 7 64 67 116 1.61e-14 65.5
MsG0680035905.01.T02 MTR_6g091700 41.538 65 37 1 7 71 46 109 2.73e-13 61.6
MsG0680035905.01.T02 MTR_6g091730 43.750 64 35 1 12 75 36 98 2.89e-12 58.2
MsG0680035905.01.T01 MTR_6g092790 98.693 153 2 0 1 153 5 157 2.31e-112 315
MsG0680035905.01.T01 MTR_8g101550 62.676 142 53 0 8 149 4 145 6.94e-65 195
MsG0680035905.01.T01 MTR_6g091720 45.122 82 35 2 35 116 30 101 9.62e-18 75.1
MsG0680035905.01.T01 MTR_6g091750 36.364 110 54 3 42 145 52 151 7.66e-17 73.2
MsG0680035905.01.T01 MTR_6g091700 42.697 89 41 2 3 91 1 79 4.35e-15 68.2
MsG0680035905.01.T01 MTR_6g091730 41.538 65 36 1 63 127 36 98 3.25e-11 57.0
MsG0680035905.01.T03 MTR_6g092790 100.000 43 0 0 1 43 115 157 3.75e-26 92.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035905.01.T02 AT2G06925 75.248 101 24 1 2 101 48 148 9.24e-54 164
MsG0680035905.01.T02 AT2G06925 75.258 97 23 1 2 97 48 144 7.21e-51 157
MsG0680035905.01.T02 AT4G29460 52.632 57 19 1 7 63 43 91 7.80e-16 69.3
MsG0680035905.01.T02 AT4G29470 52.632 57 19 1 7 63 43 91 2.96e-15 67.8
MsG0680035905.01.T02 AT4G29470 52.632 57 19 1 7 63 43 91 2.96e-15 67.8
MsG0680035905.01.T02 AT2G19690 51.786 56 26 1 7 62 45 99 9.20e-15 65.9
MsG0680035905.01.T02 AT2G19690 51.786 56 26 1 7 62 45 99 1.02e-14 65.5
MsG0680035905.01.T02 AT2G19690 64.103 39 14 0 7 45 45 83 5.94e-14 62.4
MsG0680035905.01.T01 AT2G06925 65.563 151 45 1 3 153 5 148 1.78e-71 212
MsG0680035905.01.T01 AT2G06925 65.306 147 44 1 3 149 5 144 2.23e-68 204
MsG0680035905.01.T01 AT2G19690 54.839 62 27 1 35 96 23 83 3.07e-18 75.5
MsG0680035905.01.T01 AT2G19690 46.250 80 40 2 35 114 23 99 4.77e-18 75.9
MsG0680035905.01.T01 AT2G19690 46.250 80 40 2 35 114 23 99 5.55e-18 75.9
MsG0680035905.01.T01 AT4G29460 46.914 81 40 2 37 117 23 100 7.14e-18 76.6
MsG0680035905.01.T01 AT4G29470 42.268 97 52 3 21 117 8 100 5.82e-17 74.3
MsG0680035905.01.T01 AT4G29470 42.268 97 52 3 21 117 8 100 5.82e-17 74.3
MsG0680035905.01.T03 AT2G06925 67.442 43 14 0 1 43 106 148 3.25e-15 64.3
MsG0680035905.01.T03 AT2G06925 66.667 39 13 0 1 39 106 144 2.25e-12 57.0

Find 18 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTCATGGAAGCTGCTTTAC+TGG 0.266457 6:+114263482 MsG0680035905.01.T01:CDS
GTGCCTCCATTGTTGAGATA+TGG 0.393871 6:+114262364 MsG0680035905.01.T01:intron
TGTGAGTCAAGTTTCTGTTC+AGG 0.441225 6:+114262270 MsG0680035905.01.T01:CDS
CATGTGTTTCCTTTGAATGT+AGG 0.457173 6:-114263426 None:intergenic
CATGTTTATGCACTTAACAT+AGG 0.509197 6:+114262102 MsG0680035905.01.T01:CDS
ATGGAAGCTGCTTTACTGGC+TGG 0.510582 6:+114263486 MsG0680035905.01.T01:CDS
ACAAAGGCTCCTACATTCAA+AGG 0.530546 6:+114263417 MsG0680035905.01.T01:CDS
TACTGTGGACTATTGTATAG+TGG 0.545854 6:+114262391 MsG0680035905.01.T01:CDS
CTTCAATAACATCATCTGCT+TGG 0.548099 6:-114263449 None:intergenic
TGAAGTTATCAAGATTGTCA+TGG 0.550055 6:+114263467 MsG0680035905.01.T01:CDS
CTATTGTATAGTGGATGCCC+TGG 0.587699 6:+114262400 MsG0680035905.01.T01:CDS
AACATAGGTGCTGAAACCAC+TGG 0.598513 6:+114262117 MsG0680035905.01.T01:CDS
TACTTTCCATATCTCAACAA+TGG 0.617587 6:-114262370 None:intergenic
GTTAAGTGCATAAACATGAG+TGG 0.652688 6:-114262097 None:intergenic
AACTGAAACAGCTACTCCAG+TGG 0.671963 6:-114262133 None:intergenic
TTTCCATATCTCAACAATGG+AGG 0.673712 6:-114262367 None:intergenic
TTGAGATATGGAAAGTACTG+TGG 0.689516 6:+114262376 MsG0680035905.01.T01:CDS
CAAGCATCTAGACCATCACA+AGG 0.698237 6:-114262430 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAATTTAACATATATATT+AGG + Chr6:114262745-114262764 MsG0680035905.01.T01:intron 10.0%
!!! TTAATTAATATGTTTAGAAT+TGG - Chr6:114262184-114262203 None:intergenic 10.0%
!! AAATAAAATAAAGATATGGT+AGG + Chr6:114262827-114262846 MsG0680035905.01.T01:intron 15.0%
!! ACACAAATAAAATAAAGATA+TGG + Chr6:114262823-114262842 MsG0680035905.01.T01:intron 15.0%
!!! AAATTCTAAAATATGTTATC+AGG - Chr6:114262678-114262697 None:intergenic 15.0%
!! AAATGAAGTAATTAGATGAA+GGG - Chr6:114262961-114262980 None:intergenic 20.0%
!! ACTAGTAAATAGTATTAGTT+TGG - Chr6:114263065-114263084 None:intergenic 20.0%
!! ATTATCAAATGTAATGGTTA+TGG - Chr6:114263107-114263126 None:intergenic 20.0%
!! CATAAGATTAATAGTTACTA+AGG - Chr6:114263171-114263190 None:intergenic 20.0%
!! TATGGATAGTCAAAAAATTT+AGG + Chr6:114262649-114262668 MsG0680035905.01.T01:intron 20.0%
!! TTATCAAATGTAATGGTTAT+GGG - Chr6:114263106-114263125 None:intergenic 20.0%
!!! ATCTAACATTGTTATTGATT+AGG + Chr6:114262586-114262605 MsG0680035905.01.T01:intron 20.0%
!!! ATTGGTTAGTCTTTATTTAT+GGG - Chr6:114262936-114262955 None:intergenic 20.0%
!!! TTTTTAACCAGTATACTAAT+TGG + Chr6:114262883-114262902 MsG0680035905.01.T01:intron 20.0%
!!! TTTTTTGCTATACTTTATGT+TGG + Chr6:114263141-114263160 MsG0680035905.01.T01:intron 20.0%
! ATTCAATCCTAAAAGCTATA+TGG + Chr6:114262631-114262650 MsG0680035905.01.T01:intron 25.0%
! GAAATGAAGTAATTAGATGA+AGG - Chr6:114262962-114262981 None:intergenic 25.0%
! GAATCAGATGATACTAAAAA+TGG - Chr6:114262560-114262579 None:intergenic 25.0%
! TAAAATAAAGATATGGTAGG+TGG + Chr6:114262830-114262849 MsG0680035905.01.T01:intron 25.0%
! TAGCAAAAAAAAATTAGTCC+TGG - Chr6:114263131-114263150 None:intergenic 25.0%
! TATATGTGTCAATATCATGT+TGG - Chr6:114263253-114263272 None:intergenic 25.0%
! TCATCTAATTACTTCATTTC+TGG + Chr6:114262962-114262981 MsG0680035905.01.T01:intron 25.0%
!!! ATAGCTTTTAGGATTGAATT+AGG - Chr6:114262630-114262649 None:intergenic 25.0%
!!! CATTGGTTAGTCTTTATTTA+TGG - Chr6:114262937-114262956 None:intergenic 25.0%
!!! TTTTTGAAAATTGAGCTGAT+TGG + Chr6:114262333-114262352 MsG0680035905.01.T01:intron 25.0%
ATCAGTAAGTATTATTGCAG+TGG + Chr6:114262775-114262794 MsG0680035905.01.T01:intron 30.0%
CATGTTTATGCACTTAACAT+AGG + Chr6:114262102-114262121 MsG0680035905.01.T01:CDS 30.0%
CCTGGAATTATCAAATGTAA+TGG - Chr6:114263113-114263132 None:intergenic 30.0%
CTCTTTGCTCTACAAAAAAA+AGG - Chr6:114262238-114262257 None:intergenic 30.0%
GGAAAAATTTAGGAACACAA+AGG + Chr6:114263401-114263420 MsG0680035905.01.T01:CDS 30.0%
TACTTTCCATATCTCAACAA+TGG - Chr6:114262373-114262392 None:intergenic 30.0%
TAGACTGCATGGAAAAATTT+AGG + Chr6:114263391-114263410 MsG0680035905.01.T01:CDS 30.0%
TGAAGTTATCAAGATTGTCA+TGG + Chr6:114263467-114263486 MsG0680035905.01.T01:CDS 30.0%
TGTCAATATCATGTTGGTAT+CGG - Chr6:114263247-114263266 None:intergenic 30.0%
! CCATTACATTTGATAATTCC+AGG + Chr6:114263110-114263129 MsG0680035905.01.T01:intron 30.0%
! TGACTATCCATATAGCTTTT+AGG - Chr6:114262641-114262660 None:intergenic 30.0%
! TTTGCTATACTTTATGTTGG+TGG + Chr6:114263144-114263163 MsG0680035905.01.T01:intron 30.0%
!! TATTTTTGACTGTGACACAT+TGG - Chr6:114262471-114262490 None:intergenic 30.0%
AATCCTCTTAAGCATAGTCT+TGG - Chr6:114263021-114263040 None:intergenic 35.0%
AGCTAGCTAATGATTAATGC+TGG + Chr6:114263320-114263339 MsG0680035905.01.T01:intron 35.0%
CATGTGTTTCCTTTGAATGT+AGG - Chr6:114263429-114263448 None:intergenic 35.0%
CTTCAATAACATCATCTGCT+TGG - Chr6:114263452-114263471 None:intergenic 35.0%
GTAATTAGATGAAGGGTCAT+TGG - Chr6:114262954-114262973 None:intergenic 35.0%
GTTAAGTGCATAAACATGAG+TGG - Chr6:114262100-114262119 None:intergenic 35.0%
TAAACTTCATAGCAGGTACA+AGG - Chr6:114262909-114262928 None:intergenic 35.0%
TACAAGGCCAATTAGTATAC+TGG - Chr6:114262893-114262912 None:intergenic 35.0%
TGTGTGTGTTTGATATCAAG+TGG - Chr6:114262808-114262827 None:intergenic 35.0%
TTTCAGACTGAATGTCTGTT+TGG + Chr6:114263201-114263220 MsG0680035905.01.T01:intron 35.0%
TTTCCATATCTCAACAATGG+AGG - Chr6:114262370-114262389 None:intergenic 35.0%
! TACTGTGGACTATTGTATAG+TGG + Chr6:114262391-114262410 MsG0680035905.01.T01:CDS 35.0%
!! TTGAGATATGGAAAGTACTG+TGG + Chr6:114262376-114262395 MsG0680035905.01.T01:CDS 35.0%
ACAAAGGCTCCTACATTCAA+AGG + Chr6:114263417-114263436 MsG0680035905.01.T01:CDS 40.0%
ACTCCAAGACTATGCTTAAG+AGG + Chr6:114263015-114263034 MsG0680035905.01.T01:intron 40.0%
CCAAACATTCATAGACTGCA+TGG + Chr6:114263380-114263399 MsG0680035905.01.T01:CDS 40.0%
CCATGCAGTCTATGAATGTT+TGG - Chr6:114263383-114263402 None:intergenic 40.0%
GGGTACATAAACTTCATAGC+AGG - Chr6:114262916-114262935 None:intergenic 40.0%
GTTTGGTATCCTACACATGT+TGG + Chr6:114263218-114263237 MsG0680035905.01.T01:intron 40.0%
TGTGAGTCAAGTTTCTGTTC+AGG + Chr6:114262270-114262289 MsG0680035905.01.T01:CDS 40.0%
! CATCACAAGGTTTTTCACCA+GGG - Chr6:114262420-114262439 None:intergenic 40.0%
! TAAGCATAGTCTTGGAGTGA+AGG - Chr6:114263013-114263032 None:intergenic 40.0%
AACTGAAACAGCTACTCCAG+TGG - Chr6:114262136-114262155 None:intergenic 45.0%
AGTCTTGGAGTGAAGGAGAA+AGG - Chr6:114263006-114263025 None:intergenic 45.0%
CAAGCATCTAGACCATCACA+AGG - Chr6:114262433-114262452 None:intergenic 45.0%
CCTGGTGAAAAACCTTGTGA+TGG + Chr6:114262418-114262437 MsG0680035905.01.T01:CDS 45.0%
CTTAGGTAGTCATCTGCAGA+AGG - Chr6:114263348-114263367 None:intergenic 45.0%
GTGCCTCCATTGTTGAGATA+TGG + Chr6:114262364-114262383 MsG0680035905.01.T01:intron 45.0%
TATCGGACACCAACATGTGT+AGG - Chr6:114263230-114263249 None:intergenic 45.0%
! CCATCACAAGGTTTTTCACC+AGG - Chr6:114262421-114262440 None:intergenic 45.0%
! TGTCATGGAAGCTGCTTTAC+TGG + Chr6:114263482-114263501 MsG0680035905.01.T01:CDS 45.0%
!! AACATAGGTGCTGAAACCAC+TGG + Chr6:114262117-114262136 MsG0680035905.01.T01:CDS 45.0%
!! CTATTGTATAGTGGATGCCC+TGG + Chr6:114262400-114262419 MsG0680035905.01.T01:CDS 45.0%
! ATGGAAGCTGCTTTACTGGC+TGG + Chr6:114263486-114263505 MsG0680035905.01.T01:CDS 50.0%
!! TTTGGCTGCACTGCTGACTT+AGG - Chr6:114263365-114263384 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr6 gene 114262039 114263530 114262039 ID=MsG0680035905.01;Name=MsG0680035905.01
Chr6 mRNA 114262039 114263530 114262039 ID=MsG0680035905.01.T01;Parent=MsG0680035905.01;Name=MsG0680035905.01.T01;_AED=0.46;_eAED=0.47;_QI=0|0|0|1|0|0|4|0|153
Chr6 exon 114262039 114262155 114262039 ID=MsG0680035905.01.T01:exon:10170;Parent=MsG0680035905.01.T01
Chr6 exon 114262249 114262291 114262249 ID=MsG0680035905.01.T01:exon:10171;Parent=MsG0680035905.01.T01
Chr6 exon 114262365 114262490 114262365 ID=MsG0680035905.01.T01:exon:10172;Parent=MsG0680035905.01.T01
Chr6 CDS 114262039 114262155 114262039 ID=MsG0680035905.01.T01:cds;Parent=MsG0680035905.01.T01
Chr6 CDS 114262249 114262291 114262249 ID=MsG0680035905.01.T01:cds;Parent=MsG0680035905.01.T01
Chr6 CDS 114262365 114262490 114262365 ID=MsG0680035905.01.T01:cds;Parent=MsG0680035905.01.T01
Chr6 CDS 114263355 114263530 114263355 ID=MsG0680035905.01.T01:cds;Parent=MsG0680035905.01.T01
Chr6 mRNA 114262358 114263530 114262358 ID=MsG0680035905.01.T02;Parent=MsG0680035905.01;Name=MsG0680035905.01.T02;_AED=0.47;_eAED=0.48;_QI=0|0|0|1|0|0|2|0|101
Chr6 exon 114262358 114262487 114262358 ID=MsG0680035905.01.T02:exon:10174;Parent=MsG0680035905.01.T02
Chr6 CDS 114262358 114262487 114262358 ID=MsG0680035905.01.T02:cds;Parent=MsG0680035905.01.T02
Chr6 CDS 114263355 114263530 114263355 ID=MsG0680035905.01.T02:cds;Parent=MsG0680035905.01.T02
Chr6 mRNA 114263399 114263530 114263399 ID=MsG0680035905.01.T03;Parent=MsG0680035905.01;Name=MsG0680035905.01.T03;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|43
Chr6 exon 114263399 114263530 114263399 ID=MsG0680035905.01.T03:exon:10175;Parent=MsG0680035905.01.T03
Chr6 CDS 114263399 114263530 114263399 ID=MsG0680035905.01.T03:cds;Parent=MsG0680035905.01.T03
Gene Sequence

>MsG0680035905.01.T02

TTAACAGTGCCTCCATTGTTGAGATATGGAAAGTACTGTGGACTATTGTATAGTGGATGCCCTGGTGAAAAACCTTGTGATGGTCTAGATGCTTGTTGTATGAAGCATGACCAATGTGTCACAGTCAAAAATGACTACCTAAGTCAGCAGTGCAGCCAAACATTCATAGACTGCATGGAAAAATTTAGGAACACAAAGGCTCCTACATTCAAAGGAAACACATGCCAAGCAGATGATGTTATTGAAGTTATCAAGATTGTCATGGAAGCTGCTTTACTGGCTGGAAGAGTTTTACACAAACCCTAA

>MsG0680035905.01.T01

ATGGTTCTAATAAAGTATTTTGTTCTCTTATTTTTCACTTTTGAATTCAATTTGTTAACCACTCATGTTTATGCACTTAACATAGGTGCTGAAACCACTGGAGTAGCTGTTTCAGTTAGCAAAGAGTGTAGCAGACAATGTGAGTCAAGTTTCTGTTCAGTGCCTCCATTGTTGAGATATGGAAAGTACTGTGGACTATTGTATAGTGGATGCCCTGGTGAAAAACCTTGTGATGGTCTAGATGCTTGTTGTATGAAGCATGACCAATGTGTCACAGTCAAAAATAATGACTACCTAAGTCAGCAGTGCAGCCAAACATTCATAGACTGCATGGAAAAATTTAGGAACACAAAGGCTCCTACATTCAAAGGAAACACATGCCAAGCAGATGATGTTATTGAAGTTATCAAGATTGTCATGGAAGCTGCTTTACTGGCTGGAAGAGTTTTACACAAACCCTAA

>MsG0680035905.01.T03

ATGGAAAAATTTAGGAACACAAAGGCTCCTACATTCAAAGGAAACACATGCCAAGCAGATGATGTTATTGAAGTTATCAAGATTGTCATGGAAGCTGCTTTACTGGCTGGAAGAGTTTTACACAAACCCTAA

Protein sequence

>MsG0680035905.01.T02

LTVPPLLRYGKYCGLLYSGCPGEKPCDGLDACCMKHDQCVTVKNDYLSQQCSQTFIDCMEKFRNTKAPTFKGNTCQADDVIEVIKIVMEAALLAGRVLHKP*

>MsG0680035905.01.T01

MVLIKYFVLLFFTFEFNLLTTHVYALNIGAETTGVAVSVSKECSRQCESSFCSVPPLLRYGKYCGLLYSGCPGEKPCDGLDACCMKHDQCVTVKNNDYLSQQCSQTFIDCMEKFRNTKAPTFKGNTCQADDVIEVIKIVMEAALLAGRVLHKP*

>MsG0680035905.01.T03

MEKFRNTKAPTFKGNTCQADDVIEVIKIVMEAALLAGRVLHKP*