AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018164.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018164.01.T01 MTR_4g006830 97.222 144 4 0 35 178 1 144 7.68e-100 284
MsG0480018164.01.T01 MTR_5g012890 96.000 125 5 0 54 178 18 142 4.59e-85 247
MsG0480018164.01.T01 MTR_8g093770 92.063 126 10 0 53 178 18 143 7.43e-83 241
MsG0480018164.01.T01 MTR_5g012890 91.111 90 8 0 54 143 18 107 1.92e-55 171
MsG0480018164.01.T01 MTR_4g073510 70.370 54 16 0 35 88 1 54 1.97e-18 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018164.01.T01 AT2G32060 72.800 125 33 1 54 178 21 144 1.00e-63 193
MsG0480018164.01.T01 AT2G32060 72.800 125 33 1 54 178 21 144 1.00e-63 193
MsG0480018164.01.T01 AT2G32060 72.800 125 33 1 54 178 21 144 1.00e-63 193
MsG0480018164.01.T01 AT1G15930 74.400 125 31 1 54 178 21 144 1.10e-62 190
MsG0480018164.01.T01 AT1G15930 74.400 125 31 1 54 178 21 144 1.10e-62 190

Find 47 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGGCCGTGGTCAGGATTT+TGG 0.169284 4:+1093874 MsG0480018164.01.T01:intron
ACAAAAGTGATTAACTAAAT+TGG 0.262326 4:-1094094 None:intergenic
CCTGTTGCCGCCCCTGCTGC+TGG 0.273301 4:+1093090 MsG0480018164.01.T01:CDS
GCCCCTGCTGCTGGAATTCC+AGG 0.275871 4:+1093099 MsG0480018164.01.T01:CDS
TCTAAGTAACAATTCATCTA+TGG 0.290775 4:+1093011 MsG0480018164.01.T01:CDS
CTTGTGTTGTTCTGTATGTT+TGG 0.319265 4:+1092964 MsG0480018164.01.T01:CDS
CCAGCAGCAGGGGCGGCAAC+AGG 0.352403 4:-1093090 None:intergenic
TATTGAATAATTCAAATCCT+TGG 0.359044 4:-1094039 None:intergenic
TCGCCTGGAATTCCAGCAGC+AGG 0.403859 4:-1093102 None:intergenic
ATGATATCCATTGGCTCGCC+TGG 0.406108 4:-1093117 None:intergenic
ATTGAATAATTCAAATCCTT+GGG 0.424875 4:-1094038 None:intergenic
TGCAAAGACACTTGGAGAAT+GGG 0.435359 4:+1093338 MsG0480018164.01.T01:CDS
GTTGTTCTGTATGTTTGGTC+TGG 0.443984 4:+1092969 MsG0480018164.01.T01:CDS
TTGACGAGTTTAACATAGTC+AGG 0.447855 4:-1093270 None:intergenic
GTGCAAAGACACTTGGAGAA+TGG 0.453891 4:+1093337 MsG0480018164.01.T01:CDS
CTGCTCCTGTGTGGTGGTCA+AGG 0.470678 4:+1093779 MsG0480018164.01.T01:CDS
GTGCTGCAGAACAACATTGT+AGG 0.480720 4:-1093911 None:intergenic
CTGCCTTGCAACTTGTGCTT+AGG 0.487542 4:+1093142 MsG0480018164.01.T01:CDS
TAGTTGGTGTTATCTTCCCA+AGG 0.498568 4:+1094022 MsG0480018164.01.T01:three_prime_UTR
GGAAAAGCTAGGAAGGTAAC+TGG 0.499679 4:+1093756 MsG0480018164.01.T01:CDS
CGCCTGGAATTCCAGCAGCA+GGG 0.501362 4:-1093101 None:intergenic
CTTGCACGTGGCCTCCATGA+AGG 0.502412 4:+1093186 MsG0480018164.01.T01:CDS
CTTAGGAAGTCACTGGCATA+TGG 0.527492 4:+1093159 MsG0480018164.01.T01:CDS
TGGAATTCCAGCAGCAGGGG+CGG 0.535141 4:-1093097 None:intergenic
GCCTGGAATTCCAGCAGCAG+GGG 0.541122 4:-1093100 None:intergenic
CGAGTTTAACATAGTCAGGC+TGG 0.546098 4:-1093266 None:intergenic
GTTCCTAGTGCAAAGACACT+TGG 0.547315 4:+1093330 MsG0480018164.01.T01:CDS
TCTCCAAGTGTCTTTGCACT+AGG 0.553750 4:-1093333 None:intergenic
AGGAAGTCACTGGCATATGG+TGG 0.559341 4:+1093162 MsG0480018164.01.T01:CDS
AACTGGCTGCTCCTGTGTGG+TGG 0.564611 4:+1093773 MsG0480018164.01.T01:CDS
AGAAGCAAAATTCATTCTCA+AGG 0.566617 4:-1094000 None:intergenic
AAGGCAGTCATGATATCCAT+TGG 0.578468 4:-1093126 None:intergenic
ACTTGTGCTTAGGAAGTCAC+TGG 0.586574 4:+1093152 MsG0480018164.01.T01:CDS
CTCCGAGGGAAAAGCTAGGA+AGG 0.586953 4:+1093749 MsG0480018164.01.T01:CDS
TGGAATTCCAGGCGAGCCAA+TGG 0.589710 4:+1093110 MsG0480018164.01.T01:CDS
CTTCCTAAGCACAAGTTGCA+AGG 0.603957 4:-1093145 None:intergenic
AAGACACTTGGAGAATGGGC+CGG 0.606076 4:+1093342 MsG0480018164.01.T01:CDS
TGACTATGTTAAACTCGTCA+AGG 0.611176 4:+1093272 MsG0480018164.01.T01:CDS
GCATATGGTGGGCTTGCACG+TGG 0.612030 4:+1093174 MsG0480018164.01.T01:CDS
GGAAGTCACTGGCATATGGT+GGG 0.612935 4:+1093163 MsG0480018164.01.T01:CDS
TTGACTCCGAGGGAAAAGCT+AGG 0.623449 4:+1093745 MsG0480018164.01.T01:CDS
GGTAACTGGCTGCTCCTGTG+TGG 0.642490 4:+1093770 MsG0480018164.01.T01:CDS
ATACAGAACAACACAAGACA+CGG 0.647902 4:-1092958 None:intergenic
AAAGTAATTGAGAAGAACAC+AGG 0.649204 4:+1093213 MsG0480018164.01.T01:CDS
TTGTGTAAGATTGACTCCGA+GGG 0.654398 4:+1093735 MsG0480018164.01.T01:CDS
GTTGTGTAAGATTGACTCCG+AGG 0.676451 4:+1093734 MsG0480018164.01.T01:intron
TTATACCTTGACCACCACAC+AGG 0.686928 4:-1093784 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTGTATTAATTATTGT+TGG + Chr4:1093698-1093717 MsG0480018164.01.T01:intron 10.0%
!!! TTGTAATGTGTTTTTATTAT+TGG - Chr4:1093534-1093553 None:intergenic 15.0%
!! AACACATTACAAAAAATGAA+TGG + Chr4:1093542-1093561 MsG0480018164.01.T01:intron 20.0%
!! ACAAAAGTGATTAACTAAAT+TGG - Chr4:1094097-1094116 None:intergenic 20.0%
!! ACACATTACAAAAAATGAAT+GGG + Chr4:1093543-1093562 MsG0480018164.01.T01:intron 20.0%
!! AGTTCAATTCAAATATAGTT+TGG - Chr4:1093492-1093511 None:intergenic 20.0%
!! ATTGAATAATTCAAATCCTT+GGG - Chr4:1094041-1094060 None:intergenic 20.0%
!! TATTGAATAATTCAAATCCT+TGG - Chr4:1094042-1094061 None:intergenic 20.0%
!! TATTTACTTGTTTATGCATT+GGG + Chr4:1093424-1093443 MsG0480018164.01.T01:intron 20.0%
!! TTATTTACTTGTTTATGCAT+TGG + Chr4:1093423-1093442 MsG0480018164.01.T01:intron 20.0%
!!! AGATATGCTAATTAATTTTG+TGG - Chr4:1093643-1093662 None:intergenic 20.0%
!!! TTTCTTTCATTTTCATTATG+TGG + Chr4:1093973-1093992 MsG0480018164.01.T01:three_prime_UTR 20.0%
!!! TTTTCTTTTCTTTTTTCGAT+TGG + Chr4:1093825-1093844 MsG0480018164.01.T01:intron 20.0%
! CAAGCAAAATTAAAGAATGA+TGG + Chr4:1093581-1093600 MsG0480018164.01.T01:intron 25.0%
! TCTAAGTAACAATTCATCTA+TGG + Chr4:1093011-1093030 MsG0480018164.01.T01:CDS 25.0%
! TGAAAGAAAAACACAATGTA+TGG - Chr4:1093962-1093981 None:intergenic 25.0%
!! TAATTAATTTTGTGGTGTAG+TGG - Chr4:1093635-1093654 None:intergenic 25.0%
!! TTAACGGATATCACATTTAT+TGG + Chr4:1093382-1093401 MsG0480018164.01.T01:intron 25.0%
!!! GTAAGTCTTCTGAAATTTAA+CGG + Chr4:1093366-1093385 MsG0480018164.01.T01:intron 25.0%
AAAGTAATTGAGAAGAACAC+AGG + Chr4:1093213-1093232 MsG0480018164.01.T01:CDS 30.0%
AAATTTCAGAAGACTTACAC+CGG - Chr4:1093364-1093383 None:intergenic 30.0%
AGAAGCAAAATTCATTCTCA+AGG - Chr4:1094003-1094022 None:intergenic 30.0%
ATTACAAAAAATGAATGGGC+AGG + Chr4:1093547-1093566 MsG0480018164.01.T01:intron 30.0%
! CAATATGATTTTGCTACTCT+CGG + Chr4:1094057-1094076 MsG0480018164.01.T01:three_prime_UTR 30.0%
! GAATGAATTTTGCTTCTAGT+TGG + Chr4:1094006-1094025 MsG0480018164.01.T01:three_prime_UTR 30.0%
ATACAGAACAACACAAGACA+CGG - Chr4:1092961-1092980 None:intergenic 35.0%
CACATTCAACAGTTAAAACC+AGG - Chr4:1093858-1093877 None:intergenic 35.0%
TATGCATTGGGATACTTATC+AGG + Chr4:1093436-1093455 MsG0480018164.01.T01:intron 35.0%
TGACTATGTTAAACTCGTCA+AGG + Chr4:1093272-1093291 MsG0480018164.01.T01:CDS 35.0%
TTGACGAGTTTAACATAGTC+AGG - Chr4:1093273-1093292 None:intergenic 35.0%
! CTTGTGTTGTTCTGTATGTT+TGG + Chr4:1092964-1092983 MsG0480018164.01.T01:CDS 35.0%
! TTAATTTTGTGGTGTAGTGG+TGG - Chr4:1093632-1093651 None:intergenic 35.0%
!! CAATTACTTTTGCACCTTCA+TGG - Chr4:1093203-1093222 None:intergenic 35.0%
!! GATATTTTTGCTATGCAGTG+AGG + Chr4:1093047-1093066 MsG0480018164.01.T01:CDS 35.0%
!!! CTGGTTTTAACTGTTGAATG+TGG + Chr4:1093856-1093875 MsG0480018164.01.T01:intron 35.0%
AAGGCAGTCATGATATCCAT+TGG - Chr4:1093129-1093148 None:intergenic 40.0%
GTCAATCTTACACAACTGCA+AGG - Chr4:1093730-1093749 None:intergenic 40.0%
GTTGTTCTGTATGTTTGGTC+TGG + Chr4:1092969-1092988 MsG0480018164.01.T01:CDS 40.0%
TGCAAAGACACTTGGAGAAT+GGG + Chr4:1093338-1093357 MsG0480018164.01.T01:CDS 40.0%
! TTGTGTAAGATTGACTCCGA+GGG + Chr4:1093735-1093754 MsG0480018164.01.T01:CDS 40.0%
!! TAGTTGGTGTTATCTTCCCA+AGG + Chr4:1094022-1094041 MsG0480018164.01.T01:three_prime_UTR 40.0%
!! TTACTTTTGCACCTTCATGG+AGG - Chr4:1093200-1093219 None:intergenic 40.0%
!! TTTTCGATTGGTGTTTTGCC+TGG + Chr4:1093837-1093856 MsG0480018164.01.T01:intron 40.0%
ACTTGTGCTTAGGAAGTCAC+TGG + Chr4:1093152-1093171 MsG0480018164.01.T01:CDS 45.0%
CGAGTTTAACATAGTCAGGC+TGG - Chr4:1093269-1093288 None:intergenic 45.0%
CTTACACAACTGCAAGGAGA+AGG - Chr4:1093724-1093743 None:intergenic 45.0%
CTTAGGAAGTCACTGGCATA+TGG + Chr4:1093159-1093178 MsG0480018164.01.T01:CDS 45.0%
CTTCCTAAGCACAAGTTGCA+AGG - Chr4:1093148-1093167 None:intergenic 45.0%
GGAAAAGCTAGGAAGGTAAC+TGG + Chr4:1093756-1093775 MsG0480018164.01.T01:CDS 45.0%
GTGCAAAGACACTTGGAGAA+TGG + Chr4:1093337-1093356 MsG0480018164.01.T01:CDS 45.0%
GTGCTGCAGAACAACATTGT+AGG - Chr4:1093914-1093933 None:intergenic 45.0%
GTTCCTAGTGCAAAGACACT+TGG + Chr4:1093330-1093349 MsG0480018164.01.T01:CDS 45.0%
TCTCCAAGTGTCTTTGCACT+AGG - Chr4:1093336-1093355 None:intergenic 45.0%
TTAACTGTTGAATGTGGCCG+TGG + Chr4:1093862-1093881 MsG0480018164.01.T01:intron 45.0%
TTATACCTTGACCACCACAC+AGG - Chr4:1093787-1093806 None:intergenic 45.0%
! GTTGTGTAAGATTGACTCCG+AGG + Chr4:1093734-1093753 MsG0480018164.01.T01:intron 45.0%
!! TACCTTCCTAGCTTTTCCCT+CGG - Chr4:1093754-1093773 None:intergenic 45.0%
AAGACACTTGGAGAATGGGC+CGG + Chr4:1093342-1093361 MsG0480018164.01.T01:CDS 50.0%
AGGAAGTCACTGGCATATGG+TGG + Chr4:1093162-1093181 MsG0480018164.01.T01:CDS 50.0%
CTGCCTTGCAACTTGTGCTT+AGG + Chr4:1093142-1093161 MsG0480018164.01.T01:CDS 50.0%
GGAAGTCACTGGCATATGGT+GGG + Chr4:1093163-1093182 MsG0480018164.01.T01:CDS 50.0%
GGTGTAGTGGTGGTTCTTGT+TGG - Chr4:1093622-1093641 None:intergenic 50.0%
TTGACTCCGAGGGAAAAGCT+AGG + Chr4:1093745-1093764 MsG0480018164.01.T01:CDS 50.0%
!! ATGATATCCATTGGCTCGCC+TGG - Chr4:1093120-1093139 None:intergenic 50.0%
CCTCCCCAAAATCCTGACCA+CGG - Chr4:1093882-1093901 None:intergenic 55.0%
CTCCGAGGGAAAAGCTAGGA+AGG + Chr4:1093749-1093768 MsG0480018164.01.T01:CDS 55.0%
TGGAATTCCAGGCGAGCCAA+TGG + Chr4:1093110-1093129 MsG0480018164.01.T01:CDS 55.0%
TGTTGAATGTGGCCGTGGTC+AGG + Chr4:1093867-1093886 MsG0480018164.01.T01:intron 55.0%
! GTGGCCGTGGTCAGGATTTT+GGG + Chr4:1093875-1093894 MsG0480018164.01.T01:intron 55.0%
! TGGCCGTGGTCAGGATTTTG+GGG + Chr4:1093876-1093895 MsG0480018164.01.T01:intron 55.0%
! TGTGGCCGTGGTCAGGATTT+TGG + Chr4:1093874-1093893 MsG0480018164.01.T01:intron 55.0%
AACTGGCTGCTCCTGTGTGG+TGG + Chr4:1093773-1093792 MsG0480018164.01.T01:CDS 60.0%
CGCCTGGAATTCCAGCAGCA+GGG - Chr4:1093104-1093123 None:intergenic 60.0%
CTGCTCCTGTGTGGTGGTCA+AGG + Chr4:1093779-1093798 MsG0480018164.01.T01:CDS 60.0%
CTTGCACGTGGCCTCCATGA+AGG + Chr4:1093186-1093205 MsG0480018164.01.T01:CDS 60.0%
GCCTGGAATTCCAGCAGCAG+GGG - Chr4:1093103-1093122 None:intergenic 60.0%
GGTAACTGGCTGCTCCTGTG+TGG + Chr4:1093770-1093789 MsG0480018164.01.T01:CDS 60.0%
TCGCCTGGAATTCCAGCAGC+AGG - Chr4:1093105-1093124 None:intergenic 60.0%
TGGAATTCCAGCAGCAGGGG+CGG - Chr4:1093100-1093119 None:intergenic 60.0%
!! CCGTGGTCAGGATTTTGGGG+AGG + Chr4:1093879-1093898 MsG0480018164.01.T01:intron 60.0%
!! GCATATGGTGGGCTTGCACG+TGG + Chr4:1093174-1093193 MsG0480018164.01.T01:CDS 60.0%
GCCCCTGCTGCTGGAATTCC+AGG + Chr4:1093099-1093118 MsG0480018164.01.T01:CDS 65.0%
CCAGCAGCAGGGGCGGCAAC+AGG - Chr4:1093093-1093112 None:intergenic 75.0%
CCTGTTGCCGCCCCTGCTGC+TGG + Chr4:1093090-1093109 MsG0480018164.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 1092955 1094123 1092955 ID=MsG0480018164.01;Name=MsG0480018164.01
Chr4 mRNA 1092955 1094123 1092955 ID=MsG0480018164.01.T01;Parent=MsG0480018164.01;Name=MsG0480018164.01.T01;_AED=0.38;_eAED=0.47;_QI=0|1|0.66|1|1|1|3|175|178
Chr4 exon 1092955 1093365 1092955 ID=MsG0480018164.01.T01:exon:457;Parent=MsG0480018164.01.T01
Chr4 exon 1093735 1093800 1093735 ID=MsG0480018164.01.T01:exon:458;Parent=MsG0480018164.01.T01
Chr4 exon 1093889 1094123 1093889 ID=MsG0480018164.01.T01:exon:459;Parent=MsG0480018164.01.T01
Chr4 CDS 1092955 1093365 1092955 ID=MsG0480018164.01.T01:cds;Parent=MsG0480018164.01.T01
Chr4 CDS 1093735 1093800 1093735 ID=MsG0480018164.01.T01:cds;Parent=MsG0480018164.01.T01
Chr4 CDS 1093889 1093948 1093889 ID=MsG0480018164.01.T01:cds;Parent=MsG0480018164.01.T01
Chr4 three_prime_UTR 1093949 1094123 1093949 ID=MsG0480018164.01.T01:three_prime_utr;Parent=MsG0480018164.01.T01
Gene Sequence

>MsG0480018164.01.T01

ATGCCGTGTCTTGTGTTGTTCTGTATGTTTGGTCTGGTTGACACGAGTATCTTTTGTCTAAGTAACAATTCATCTATGGTTCGTCTTTTCATGATATTTTTGCTATGCAGTGAGGACGCTGTTGTTGCAGTTGAGCCTGTTGCCGCCCCTGCTGCTGGAATTCCAGGCGAGCCAATGGATATCATGACTGCCTTGCAACTTGTGCTTAGGAAGTCACTGGCATATGGTGGGCTTGCACGTGGCCTCCATGAAGGTGCAAAAGTAATTGAGAAGAACACAGGACAGCTTTGTGTTCTTGCAGAAGATTGTGACCAGCCTGACTATGTTAAACTCGTCAAGGCTCTTTGTGCTGAGCACAATGTTAGTCTTCTGACAGTTCCTAGTGCAAAGACACTTGGAGAATGGGCCGGTTTGTGTAAGATTGACTCCGAGGGAAAAGCTAGGAAGGTAACTGGCTGCTCCTGTGTGGTGGTCAAGGATTTTGGGGAGGAACATGAAGCCTACAATGTTGTTCTGCAGCACGTCAAAGCTCAATGA

Protein sequence

>MsG0480018164.01.T01

MPCLVLFCMFGLVDTSIFCLSNNSSMVRLFMIFLLCSEDAVVAVEPVAAPAAGIPGEPMDIMTALQLVLRKSLAYGGLARGLHEGAKVIEKNTGQLCVLAEDCDQPDYVKLVKALCAEHNVSLLTVPSAKTLGEWAGLCKIDSEGKARKVTGCSCVVVKDFGEEHEAYNVVLQHVKAQ*