Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005384.01.T01 | XP_024642001.1 | 100 | 78 | 0 | 0 | 1 | 78 | 312 | 389 | 7.72E-46 | 160 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005384.01.T01 | P35684 | 87.179 | 78 | 10 | 0 | 1 | 78 | 312 | 389 | 1.24E-42 | 145 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005384.01.T01 | G7IBY1 | 100.000 | 78 | 0 | 0 | 1 | 78 | 312 | 389 | 3.69e-46 | 160 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048040.01 | MsG0180005384.01 | 0.858554 | 7.606460e-63 | 1.948777e-59 |
| MsG0180000096.01 | MsG0180005384.01 | 0.897272 | 1.650685e-76 | 1.850502e-72 |
| MsG0180000118.01 | MsG0180005384.01 | 0.908335 | 1.912145e-81 | 3.533567e-77 |
| MsG0180001169.01 | MsG0180005384.01 | 0.816700 | 4.831188e-52 | 3.447808e-49 |
| MsG0180001829.01 | MsG0180005384.01 | 0.848195 | 7.153871e-60 | 1.302383e-56 |
| MsG0180003585.01 | MsG0180005384.01 | 0.810956 | 8.908311e-51 | 5.444976e-48 |
| MsG0180003897.01 | MsG0180005384.01 | 0.879014 | 1.760109e-69 | 9.392380e-66 |
| MsG0180004503.01 | MsG0180005384.01 | 0.825205 | 5.321865e-54 | 4.818916e-51 |
| MsG0180004625.01 | MsG0180005384.01 | 0.804959 | 1.681418e-49 | 8.787298e-47 |
| MsG0180004829.01 | MsG0180005384.01 | 0.910489 | 1.769095e-82 | 3.621547e-78 |
| MsG0180005384.01 | MsG0180005573.01 | 0.847296 | 1.265126e-59 | 2.238174e-56 |
| MsG0180005384.01 | MsG0280006301.01 | 0.856338 | 3.443158e-62 | 8.191040e-59 |
| MsG0180005384.01 | MsG0280006629.01 | 0.871396 | 7.058367e-67 | 2.842899e-63 |
| MsG0180005384.01 | MsG0280006747.01 | 0.876334 | 1.516709e-68 | 7.324509e-65 |
| MsG0180005384.01 | MsG0280006760.01 | 0.848822 | 4.796208e-60 | 8.912429e-57 |
| MsG0180005384.01 | MsG0280007055.01 | 0.959114 | 4.364714e-117 | 1.701406e-111 |
| MsG0180005384.01 | MsG0280007606.01 | 0.800861 | 1.181570e-48 | 5.561228e-46 |
| MsG0180005384.01 | MsG0280007995.01 | 0.819697 | 1.013414e-52 | 7.855072e-50 |
| MsG0180005384.01 | MsG0280010519.01 | 0.851962 | 6.304181e-61 | 1.297228e-57 |
| MsG0180005384.01 | MsG0380014507.01 | 0.826235 | 3.031769e-54 | 2.826882e-51 |
| MsG0180005384.01 | MsG0380015446.01 | 0.803693 | 3.085600e-49 | 1.560792e-46 |
| MsG0180005384.01 | MsG0380015766.01 | 0.814992 | 1.161350e-51 | 7.910085e-49 |
| MsG0180005384.01 | MsG0380015857.01 | 0.944683 | 1.240932e-103 | 1.802606e-98 |
| MsG0180005384.01 | MsG0380016433.01 | 0.805425 | 1.343129e-49 | 7.103458e-47 |
| MsG0180005384.01 | MsG0380016678.01 | 0.878882 | 1.959798e-69 | 1.040196e-65 |
| MsG0180005384.01 | MsG0380016744.01 | 0.849655 | 2.813189e-60 | 5.370650e-57 |
| MsG0180005384.01 | MsG0380016760.01 | 0.895377 | 1.015617e-75 | 1.050985e-71 |
| MsG0180005384.01 | MsG0380017155.01 | 0.844252 | 8.483357e-59 | 1.361491e-55 |
| MsG0180005384.01 | MsG0380017516.01 | 0.860535 | 1.930028e-63 | 5.293334e-60 |
| MsG0180005384.01 | MsG0380017664.01 | 0.818654 | 1.751478e-52 | 1.318795e-49 |
| MsG0180005384.01 | MsG0380018056.01 | 0.855489 | 6.097882e-62 | 1.410180e-58 |
| MsG0180005384.01 | MsG0480018164.01 | 0.942287 | 9.392582e-102 | 1.165420e-96 |
| MsG0180005384.01 | MsG0480018442.01 | -0.800693 | 1.278347e-48 | 5.990862e-46 |
| MsG0180005384.01 | MsG0480019165.01 | 0.877938 | 4.203003e-69 | 2.153816e-65 |
| MsG0180005384.01 | MsG0480019632.01 | 0.812156 | 4.885709e-51 | 3.082926e-48 |
| MsG0180005384.01 | MsG0480020796.01 | 0.904799 | 8.411434e-80 | 1.322584e-75 |
| MsG0180005384.01 | MsG0480020895.01 | 0.800473 | 1.417702e-48 | 6.606865e-46 |
| MsG0180005384.01 | MsG0480020911.01 | 0.917992 | 2.698101e-86 | 8.021004e-82 |
| MsG0180005384.01 | MsG0480021247.01 | 0.846467 | 2.132543e-59 | 3.674422e-56 |
| MsG0180005384.01 | MsG0480021279.01 | 0.887951 | 9.082002e-73 | 6.895768e-69 |
| MsG0180005384.01 | MsG0480021288.01 | 0.840482 | 8.466682e-58 | 1.207634e-54 |
| MsG0180005384.01 | MsG0480021293.01 | 0.870533 | 1.358279e-66 | 5.302024e-63 |
| MsG0180005384.01 | MsG0480021417.01 | 0.885013 | 1.172150e-71 | 7.913596e-68 |
| MsG0180005384.01 | MsG0480021985.01 | 0.800945 | 1.135596e-48 | 5.356334e-46 |
| MsG0180005384.01 | MsG0480022006.01 | 0.930640 | 1.219053e-93 | 7.328198e-89 |
| MsG0180005384.01 | MsG0480022041.01 | 0.870700 | 1.196608e-66 | 4.698870e-63 |
| MsG0180005384.01 | MsG0480022302.01 | 0.804541 | 2.055845e-49 | 1.062783e-46 |
| MsG0180005384.01 | MsG0480022363.01 | 0.861956 | 7.124654e-64 | 2.051546e-60 |
| MsG0180005384.01 | MsG0480022789.01 | 0.838262 | 3.192775e-57 | 4.253009e-54 |
| MsG0180005384.01 | MsG0480022955.01 | 0.872188 | 3.853411e-67 | 1.597429e-63 |
| MsG0180005384.01 | MsG0480023380.01 | 0.904430 | 1.237735e-79 | 1.913857e-75 |
| MsG0180005384.01 | MsG0480023774.01 | 0.931072 | 6.473218e-94 | 4.001338e-89 |
| MsG0180005384.01 | MsG0480023931.01 | 0.830143 | 3.466875e-55 | 3.621708e-52 |
| MsG0180005384.01 | MsG0480023941.01 | 0.879061 | 1.694651e-69 | 9.057442e-66 |
| MsG0180005384.01 | MsG0480023996.01 | 0.839098 | 1.941274e-57 | 2.653020e-54 |
| MsG0180005384.01 | MsG0580024059.01 | 0.854732 | 1.012001e-61 | 2.282121e-58 |
| MsG0180005384.01 | MsG0580024481.01 | 0.885649 | 6.778397e-72 | 4.690411e-68 |
| MsG0180005384.01 | MsG0580024540.01 | 0.887138 | 1.857121e-72 | 1.364225e-68 |
| MsG0180005384.01 | MsG0580024738.01 | 0.865574 | 5.348848e-65 | 1.748782e-61 |
| MsG0180005384.01 | MsG0580024894.01 | 0.912155 | 2.690607e-83 | 5.967273e-79 |
| MsG0180005384.01 | MsG0580025750.01 | 0.821676 | 3.558068e-53 | 2.914090e-50 |
| MsG0180005384.01 | MsG0580026749.01 | 0.871996 | 4.464395e-67 | 1.837167e-63 |
| MsG0180005384.01 | MsG0680031368.01 | 0.912531 | 1.751357e-83 | 3.958050e-79 |
| MsG0180005384.01 | MsG0680032459.01 | 0.819153 | 1.348446e-52 | 1.029439e-49 |
| MsG0180005384.01 | MsG0680035611.01 | 0.844495 | 7.298432e-59 | 1.180415e-55 |
| MsG0180005384.01 | MsG0780037290.01 | 0.860069 | 2.669853e-63 | 7.207113e-60 |
| MsG0180005384.01 | MsG0780038383.01 | 0.864840 | 9.100759e-65 | 2.897759e-61 |
| MsG0180005384.01 | MsG0780039937.01 | 0.813974 | 1.950136e-51 | 1.292137e-48 |
| MsG0180005384.01 | MsG0780040174.01 | 0.822774 | 1.978465e-53 | 1.671952e-50 |
| MsG0180005384.01 | MsG0780041058.01 | 0.814035 | 1.891172e-51 | 1.255160e-48 |
| MsG0180005384.01 | MsG0780041240.01 | 0.810287 | 1.242626e-50 | 7.460213e-48 |
| MsG0180005384.01 | MsG0780041311.01 | 0.811909 | 5.530090e-51 | 3.465967e-48 |
| MsG0180005384.01 | MsG0780041460.01 | -0.803068 | 4.159284e-49 | 2.070205e-46 |
| MsG0180005384.01 | MsG0780041461.01 | 0.833436 | 5.340466e-56 | 6.149882e-53 |
| MsG0180005384.01 | MsG0780041522.01 | 0.894405 | 2.544643e-75 | 2.525999e-71 |
| MsG0180005384.01 | MsG0780041790.01 | 0.911341 | 6.789732e-83 | 1.447972e-78 |
| MsG0180005384.01 | MsG0880041941.01 | 0.867879 | 9.880692e-66 | 3.503761e-62 |
| MsG0180005384.01 | MsG0880042517.01 | 0.864266 | 1.376165e-64 | 4.294708e-61 |
| MsG0180005384.01 | MsG0880042896.01 | 0.826946 | 2.052425e-54 | 1.953166e-51 |
| MsG0180005384.01 | MsG0880043076.01 | 0.856518 | 3.048508e-62 | 7.296726e-59 |
| MsG0180005384.01 | MsG0880044204.01 | 0.843964 | 1.013231e-58 | 1.611721e-55 |
| MsG0180005384.01 | MsG0880045836.01 | 0.879190 | 1.525317e-69 | 8.193689e-66 |
| MsG0180005384.01 | MsG0880046220.01 | 0.879874 | 8.721895e-70 | 4.808558e-66 |
| MsG0180005384.01 | MsG0880047310.01 | 0.851353 | 9.382301e-61 | 1.893943e-57 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005384.01.T01 | MTR_1g098540 | 100.000 | 78 | 0 | 0 | 1 | 78 | 312 | 389 | 9.35e-50 | 160 |
| MsG0180005384.01.T01 | MTR_4g094398 | 92.308 | 78 | 6 | 0 | 1 | 78 | 312 | 389 | 4.38e-47 | 154 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005384.01.T01 | AT1G43170 | 81.579 | 76 | 14 | 0 | 1 | 76 | 229 | 304 | 1.78e-40 | 134 |
| MsG0180005384.01.T01 | AT1G61580 | 82.667 | 75 | 13 | 0 | 1 | 75 | 312 | 386 | 9.52e-40 | 134 |
| MsG0180005384.01.T01 | AT1G43170 | 81.579 | 76 | 14 | 0 | 1 | 76 | 312 | 387 | 1.62e-39 | 134 |
| MsG0180005384.01.T01 | AT1G43170 | 81.579 | 76 | 14 | 0 | 1 | 76 | 312 | 387 | 1.62e-39 | 134 |
| MsG0180005384.01.T01 | AT1G43170 | 81.579 | 76 | 14 | 0 | 1 | 76 | 312 | 387 | 1.62e-39 | 134 |
| MsG0180005384.01.T01 | AT1G43170 | 81.579 | 76 | 14 | 0 | 1 | 76 | 312 | 387 | 1.62e-39 | 134 |
| MsG0180005384.01.T01 | AT1G43170 | 81.579 | 76 | 14 | 0 | 1 | 76 | 312 | 387 | 1.62e-39 | 134 |
| MsG0180005384.01.T01 | AT1G43170 | 81.579 | 76 | 14 | 0 | 1 | 76 | 312 | 387 | 1.62e-39 | 134 |
| MsG0180005384.01.T01 | AT1G43170 | 81.579 | 76 | 14 | 0 | 1 | 76 | 312 | 387 | 1.62e-39 | 134 |
| MsG0180005384.01.T01 | AT1G43170 | 81.579 | 76 | 14 | 0 | 1 | 76 | 312 | 387 | 1.62e-39 | 134 |
Find 22 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCGATACCTCTTCCAAGTT+TGG | 0.285033 | 1:-91104589 | MsG0180005384.01.T01:CDS |
| CAGAGTAATAACCCTCTTCT+TGG | 0.375680 | 1:+91104672 | None:intergenic |
| TTTGCTTCTCTGATGTTGTC+TGG | 0.376238 | 1:+91104554 | None:intergenic |
| GAAACGACCATGTCCAAACT+TGG | 0.447320 | 1:+91104576 | None:intergenic |
| ACTACCATAATTAAGCTTTG+AGG | 0.473296 | 1:+91104518 | None:intergenic |
| GACTTCTCGTCTTGCTCTTG+AGG | 0.480840 | 1:-91104629 | MsG0180005384.01.T01:CDS |
| AGAGTAATAACCCTCTTCTT+GGG | 0.492275 | 1:+91104673 | None:intergenic |
| GACGAGAAGTCTGCTTAAGA+AGG | 0.502167 | 1:+91104641 | None:intergenic |
| ATGATCAAGGGAGGCTGTGT+TGG | 0.526401 | 1:-91104697 | MsG0180005384.01.T01:CDS |
| AAGTCTGCTTAAGAAGGGAC+TGG | 0.577488 | 1:+91104647 | None:intergenic |
| ACCTCTTCCAAGTTTGGACA+TGG | 0.582976 | 1:-91104583 | MsG0180005384.01.T01:CDS |
| ACCATGTCCAAACTTGGAAG+AGG | 0.600841 | 1:+91104582 | None:intergenic |
| ATGGGTGGTTTCCCACACTA+CGG | 0.607609 | 1:-91104742 | MsG0180005384.01.T01:CDS |
| CTGTGTTGGTCCCAAGAAGA+GGG | 0.610053 | 1:-91104683 | MsG0180005384.01.T01:CDS |
| ACGAGAAGTCTGCTTAAGAA+GGG | 0.612938 | 1:+91104642 | None:intergenic |
| GCTGTGTTGGTCCCAAGAAG+AGG | 0.613945 | 1:-91104684 | MsG0180005384.01.T01:CDS |
| CCTTGACAATACCGTAGTGT+GGG | 0.616873 | 1:+91104731 | None:intergenic |
| CCCACACTACGGTATTGTCA+AGG | 0.620478 | 1:-91104731 | MsG0180005384.01.T01:CDS |
| TAGTGTGGGAAACCACCCAT+AGG | 0.626093 | 1:+91104745 | None:intergenic |
| TCCTTGACAATACCGTAGTG+TGG | 0.648748 | 1:+91104730 | None:intergenic |
| AAAGACATTACACCTATGGG+TGG | 0.665025 | 1:-91104757 | None:intergenic |
| ACACTACGGTATTGTCAAGG+AGG | 0.722752 | 1:-91104728 | MsG0180005384.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCAGAGAAGCAAAAGTTTTA+TGG | - | Chr1:91104726-91104745 | MsG0180005384.01.T01:CDS | 30.0% |
| AGAGTAATAACCCTCTTCTT+GGG | + | Chr1:91104600-91104619 | None:intergenic | 35.0% | |
| ! | GAGGATTTTCTGATGATCAA+GGG | - | Chr1:91104561-91104580 | MsG0180005384.01.T01:CDS | 35.0% |
| ACGAGAAGTCTGCTTAAGAA+GGG | + | Chr1:91104631-91104650 | None:intergenic | 40.0% | |
| ATCGATACCTCTTCCAAGTT+TGG | - | Chr1:91104681-91104700 | MsG0180005384.01.T01:CDS | 40.0% | |
| CAGAGTAATAACCCTCTTCT+TGG | + | Chr1:91104601-91104620 | None:intergenic | 40.0% | |
| TTTGCTTCTCTGATGTTGTC+TGG | + | Chr1:91104719-91104738 | None:intergenic | 40.0% | |
| ! | GATTTTCTGATGATCAAGGG+AGG | - | Chr1:91104564-91104583 | MsG0180005384.01.T01:CDS | 40.0% |
| ! | GGAGGATTTTCTGATGATCA+AGG | - | Chr1:91104560-91104579 | MsG0180005384.01.T01:CDS | 40.0% |
| AAGTCTGCTTAAGAAGGGAC+TGG | + | Chr1:91104626-91104645 | None:intergenic | 45.0% | |
| ACACTACGGTATTGTCAAGG+AGG | - | Chr1:91104542-91104561 | MsG0180005384.01.T01:CDS | 45.0% | |
| ACCATGTCCAAACTTGGAAG+AGG | + | Chr1:91104691-91104710 | None:intergenic | 45.0% | |
| ACCTCTTCCAAGTTTGGACA+TGG | - | Chr1:91104687-91104706 | MsG0180005384.01.T01:CDS | 45.0% | |
| CCTTGACAATACCGTAGTGT+GGG | + | Chr1:91104542-91104561 | None:intergenic | 45.0% | |
| GAAACGACCATGTCCAAACT+TGG | + | Chr1:91104697-91104716 | None:intergenic | 45.0% | |
| GACGAGAAGTCTGCTTAAGA+AGG | + | Chr1:91104632-91104651 | None:intergenic | 45.0% | |
| TCCTTGACAATACCGTAGTG+TGG | + | Chr1:91104543-91104562 | None:intergenic | 45.0% | |
| ATGATCAAGGGAGGCTGTGT+TGG | - | Chr1:91104573-91104592 | MsG0180005384.01.T01:CDS | 50.0% | |
| ATGGGTGGTTTCCCACACTA+CGG | - | Chr1:91104528-91104547 | MsG0180005384.01.T01:CDS | 50.0% | |
| CCCACACTACGGTATTGTCA+AGG | - | Chr1:91104539-91104558 | MsG0180005384.01.T01:CDS | 50.0% | |
| GACTTCTCGTCTTGCTCTTG+AGG | - | Chr1:91104641-91104660 | MsG0180005384.01.T01:CDS | 50.0% | |
| ! | CTGTGTTGGTCCCAAGAAGA+GGG | - | Chr1:91104587-91104606 | MsG0180005384.01.T01:CDS | 50.0% |
| ! | GCTGTGTTGGTCCCAAGAAG+AGG | - | Chr1:91104586-91104605 | MsG0180005384.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 91104528 | 91104764 | 91104528 | ID=MsG0180005384.01;Name=MsG0180005384.01 |
| Chr1 | mRNA | 91104528 | 91104764 | 91104528 | ID=MsG0180005384.01.T01;Parent=MsG0180005384.01;Name=MsG0180005384.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|78 |
| Chr1 | exon | 91104528 | 91104764 | 91104528 | ID=MsG0180005384.01.T01:exon:38797;Parent=MsG0180005384.01.T01 |
| Chr1 | CDS | 91104528 | 91104764 | 91104528 | ID=MsG0180005384.01.T01:cds;Parent=MsG0180005384.01.T01 |
| Gene Sequence |
| Protein sequence |
