AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022797.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022797.01.T01 MTR_7g021620 97.500 320 8 0 1 320 1 320 0.0 622
MsG0480022797.01.T01 MTR_4g096890 95.312 320 15 0 1 320 1 320 0.0 611
MsG0480022797.01.T01 MTR_1g029440 41.018 334 176 5 1 317 1 330 2.79e-62 201
MsG0480022797.01.T01 MTR_3g088975 38.253 332 187 4 1 317 1 329 2.16e-61 199
MsG0480022797.01.T01 MTR_3g088965 33.435 329 192 8 1 307 1 324 1.73e-40 144
MsG0480022797.01.T01 MTR_3g014430 60.163 123 32 4 183 300 2 112 1.95e-33 120
MsG0480022797.01.T01 MTR_4g082350 25.329 304 198 7 7 289 41 336 1.56e-16 79.7
MsG0480022797.01.T01 MTR_3g014620 73.333 45 12 0 164 208 12 56 1.29e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022797.01.T01 AT4G19950 74.766 321 80 1 1 320 1 321 5.48e-163 457
MsG0480022797.01.T01 AT5G44860 73.832 321 83 1 1 320 1 321 7.42e-162 454
MsG0480022797.01.T01 AT5G44860 73.832 321 83 1 1 320 1 321 7.42e-162 454
MsG0480022797.01.T01 AT1G31130 69.040 323 95 2 1 320 1 321 1.15e-144 410
MsG0480022797.01.T01 AT1G26650 27.815 302 189 6 12 292 26 319 6.74e-21 92.0
MsG0480022797.01.T01 AT1G69430 26.490 302 193 7 11 291 41 334 2.44e-17 82.0

Find 64 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCATTCAAATGGAAAATT+TGG 0.141896 4:+76779364 MsG0480022797.01.T01:CDS
CAAAAGAAAGAGGGAAAATT+AGG 0.157322 4:-76778542 None:intergenic
TGTTCTTGTCATTGTGAATT+TGG 0.199328 4:+76779217 MsG0480022797.01.T01:CDS
GTTTGTTTATTACTTTCTTA+TGG 0.214114 4:+76778811 MsG0480022797.01.T01:CDS
AGATTGAAGCTGAGAAATTA+AGG 0.243695 4:-76778595 None:intergenic
TCTGTGCTTGAACCTCTTTA+TGG 0.244172 4:+76779011 MsG0480022797.01.T01:CDS
GATTGTCATTGGTGGGTTCT+TGG 0.268660 4:+76779190 MsG0480022797.01.T01:CDS
GGTTAGATATGCTGCACTTT+TGG 0.290596 4:+76779076 MsG0480022797.01.T01:CDS
TTGAGGAATTGAAGTTCTTC+AGG 0.305893 4:-76778444 None:intergenic
TGTGAATTTGGTTGGGTTGT+TGG 0.309528 4:+76779229 MsG0480022797.01.T01:CDS
TTTCGTCTTTGTTCTTTGTT+TGG 0.316144 4:+76778860 MsG0480022797.01.T01:CDS
TATAACTGCTTTGTGGCATT+TGG 0.326265 4:+76778977 MsG0480022797.01.T01:CDS
GGTTAGGGTAATTAAATAGA+AGG 0.347948 4:-76778520 None:intergenic
TTTGTTTATTACTTTCTTAT+GGG 0.355976 4:+76778812 MsG0480022797.01.T01:CDS
AAAGAGGGAAAATTAGGGTT+AGG 0.378142 4:-76778536 None:intergenic
CTTGTCATTGTGAATTTGGT+TGG 0.385554 4:+76779221 MsG0480022797.01.T01:CDS
ATTTGAATGCTGCTCTTAAG+AGG 0.385789 4:-76779353 None:intergenic
AACTTTAGGAATTGCTGAAA+TGG 0.389046 4:-76778781 None:intergenic
CTTATTTGCGCGGTTATTAG+TGG 0.393312 4:+76779113 MsG0480022797.01.T01:CDS
TGTTCTTTGTTTGGTTCTGA+TGG 0.397201 4:+76778869 MsG0480022797.01.T01:CDS
TTTCTAGGATTGTCATTGGT+GGG 0.414842 4:+76779183 MsG0480022797.01.T01:CDS
GAAGAGTTATGAGTTGTTGA+AGG 0.420597 4:+76779049 MsG0480022797.01.T01:CDS
ATGTTTATATAACTGCTTTG+TGG 0.423556 4:+76778970 MsG0480022797.01.T01:CDS
AAAAGAAAGAGGGAAAATTA+GGG 0.424834 4:-76778541 None:intergenic
CTGCACAATCCTCTCATGAT+TGG 0.427622 4:+76778631 MsG0480022797.01.T01:CDS
TTGTCATTGTGAATTTGGTT+GGG 0.428320 4:+76779222 MsG0480022797.01.T01:CDS
TTATGAGTTGTTGAAGGGTA+GGG 0.428981 4:+76779055 MsG0480022797.01.T01:CDS
GCTAAGATTGCAAAAGAAAG+AGG 0.433779 4:-76778552 None:intergenic
GTTATGAGTTGTTGAAGGGT+AGG 0.441707 4:+76779054 MsG0480022797.01.T01:CDS
GTCATTGGTGGGTTCTTGGT+TGG 0.447415 4:+76779194 MsG0480022797.01.T01:CDS
TTTAGCGTGGTTGTGGTACA+TGG 0.449111 4:+76779140 MsG0480022797.01.T01:CDS
GATTGAAGCTGAGAAATTAA+GGG 0.452590 4:-76778594 None:intergenic
GTACATGGTGGTGATGGTTA+TGG 0.461928 4:+76779155 MsG0480022797.01.T01:CDS
AATTAAGGGGTGTGTGAAAA+GGG 0.462222 4:-76778580 None:intergenic
GATCATCTTGGTGGCTACCT+TGG 0.466274 4:+76779320 MsG0480022797.01.T01:CDS
TCTTAAGAGCAGCATTCAAA+TGG 0.471769 4:+76779355 MsG0480022797.01.T01:CDS
AAATTAAGGGGTGTGTGAAA+AGG 0.475519 4:-76778581 None:intergenic
AGAAAGCGAAGAGGAAAATG+AGG 0.480466 4:-76778683 None:intergenic
AAGAGGGAAAATTAGGGTTA+GGG 0.495319 4:-76778535 None:intergenic
TGGGATGTTTAGCGTGGTTG+TGG 0.508402 4:+76779133 MsG0480022797.01.T01:CDS
AAGAGTTATGAGTTGTTGAA+GGG 0.511629 4:+76779050 MsG0480022797.01.T01:CDS
AGTGCTTTACATGATCATCT+TGG 0.515132 4:+76779308 MsG0480022797.01.T01:CDS
TTAAGATGTTAGGGATGTTG+AGG 0.524756 4:-76778461 None:intergenic
TTATTTGCGCGGTTATTAGT+GGG 0.540153 4:+76779114 MsG0480022797.01.T01:CDS
GCTTTACATGATCATCTTGG+TGG 0.540813 4:+76779311 MsG0480022797.01.T01:CDS
TTCTCTCTACACCTCAAAGC+CGG 0.547939 4:+76778743 MsG0480022797.01.T01:CDS
CAATCATGAGAGGATTGTGC+AGG 0.560830 4:-76778630 None:intergenic
ATAGCAGCAAAACCATAAAG+AGG 0.564333 4:-76779023 None:intergenic
GGTGAAAACAACGGCAGCGG+TGG 0.572445 4:-76778715 None:intergenic
AAACAACGGCAGCGGTGGAA+AGG 0.574298 4:-76778710 None:intergenic
AAAGGAGAGAGAAAGCGAAG+AGG 0.574399 4:-76778692 None:intergenic
GATTGTGCAGGATCAGTGAA+AGG 0.590109 4:-76778618 None:intergenic
GTTGTGGTACATGGTGGTGA+TGG 0.590598 4:+76779149 MsG0480022797.01.T01:CDS
CTAAGATTGCAAAAGAAAGA+GGG 0.595963 4:-76778551 None:intergenic
CGGGTATTTGCTTATTTGCG+CGG 0.599347 4:+76779103 MsG0480022797.01.T01:CDS
GACGGTGAAAACAACGGCAG+CGG 0.601261 4:-76778718 None:intergenic
TAAGAGGCACATATTCTCCA+AGG 0.610436 4:-76779337 None:intergenic
AGCAGTTGATACTGACAACT+CGG 0.612984 4:+76778896 MsG0480022797.01.T01:CDS
GAGAAGAGTCCAATCATGAG+AGG 0.621578 4:-76778640 None:intergenic
ATTGAAGCTGAGAAATTAAG+GGG 0.648141 4:-76778593 None:intergenic
TATTAGTGGGATGTTTAGCG+TGG 0.653236 4:+76779127 MsG0480022797.01.T01:CDS
TGAGGTGTAGAGAGAAGCGA+CGG 0.663460 4:-76778736 None:intergenic
AGAAGCGACGGTGAAAACAA+CGG 0.686937 4:-76778724 None:intergenic
AGCGTGGTTGTGGTACATGG+TGG 0.722658 4:+76779143 MsG0480022797.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGTTTATTACTTTCTTAT+GGG + Chr4:76778812-76778831 MsG0480022797.01.T01:CDS 15.0%
!!! TAATTAAATAGAAGGTTTTA+GGG - Chr4:76778515-76778534 None:intergenic 15.0%
!! GTTTGTTTATTACTTTCTTA+TGG + Chr4:76778811-76778830 MsG0480022797.01.T01:CDS 20.0%
!!! AATTAAATAGAAGGTTTTAG+GGG - Chr4:76778514-76778533 None:intergenic 20.0%
!!! ATTGATTCTTTTAAGATGTT+AGG - Chr4:76778474-76778493 None:intergenic 20.0%
!!! GTAATTAAATAGAAGGTTTT+AGG - Chr4:76778516-76778535 None:intergenic 20.0%
!!! TTGATTCTTTTAAGATGTTA+GGG - Chr4:76778473-76778492 None:intergenic 20.0%
! AAAAGAAAGAGGGAAAATTA+GGG - Chr4:76778544-76778563 None:intergenic 25.0%
! AACAAACGCTTAAAAACTTT+AGG - Chr4:76778798-76778817 None:intergenic 25.0%
! ATGTTTATATAACTGCTTTG+TGG + Chr4:76778970-76778989 MsG0480022797.01.T01:CDS 25.0%
AAGAGTTATGAGTTGTTGAA+GGG + Chr4:76779050-76779069 MsG0480022797.01.T01:CDS 30.0%
AGATTGAAGCTGAGAAATTA+AGG - Chr4:76778598-76778617 None:intergenic 30.0%
ATTGAAGCTGAGAAATTAAG+GGG - Chr4:76778596-76778615 None:intergenic 30.0%
CAAAAGAAAGAGGGAAAATT+AGG - Chr4:76778545-76778564 None:intergenic 30.0%
CAGCATTCAAATGGAAAATT+TGG + Chr4:76779364-76779383 MsG0480022797.01.T01:CDS 30.0%
CTAAGATTGCAAAAGAAAGA+GGG - Chr4:76778554-76778573 None:intergenic 30.0%
GATTGAAGCTGAGAAATTAA+GGG - Chr4:76778597-76778616 None:intergenic 30.0%
GGTAGAAAAACTGAATGATA+AGG - Chr4:76778665-76778684 None:intergenic 30.0%
GGTTAGGGTAATTAAATAGA+AGG - Chr4:76778523-76778542 None:intergenic 30.0%
TGTTCTTGTCATTGTGAATT+TGG + Chr4:76779217-76779236 MsG0480022797.01.T01:CDS 30.0%
TTGTCATTGTGAATTTGGTT+GGG + Chr4:76779222-76779241 MsG0480022797.01.T01:CDS 30.0%
TTTCGTCTTTGTTCTTTGTT+TGG + Chr4:76778860-76778879 MsG0480022797.01.T01:CDS 30.0%
! AACTTTAGGAATTGCTGAAA+TGG - Chr4:76778784-76778803 None:intergenic 30.0%
!!! AAGGTTTTAGGGGAAATTTT+TGG - Chr4:76778504-76778523 None:intergenic 30.0%
!!! AGGTTTTAGGGGAAATTTTT+GGG - Chr4:76778503-76778522 None:intergenic 30.0%
AAAGAGGGAAAATTAGGGTT+AGG - Chr4:76778539-76778558 None:intergenic 35.0%
AAGAGGGAAAATTAGGGTTA+GGG - Chr4:76778538-76778557 None:intergenic 35.0%
AGTGCTTTACATGATCATCT+TGG + Chr4:76779308-76779327 MsG0480022797.01.T01:CDS 35.0%
ATAGCAGCAAAACCATAAAG+AGG - Chr4:76779026-76779045 None:intergenic 35.0%
ATGGTGTTTGAAAAAGACAC+CGG - Chr4:76778765-76778784 None:intergenic 35.0%
ATTTGAATGCTGCTCTTAAG+AGG - Chr4:76779356-76779375 None:intergenic 35.0%
CTTGTCATTGTGAATTTGGT+TGG + Chr4:76779221-76779240 MsG0480022797.01.T01:CDS 35.0%
GAAGAGTTATGAGTTGTTGA+AGG + Chr4:76779049-76779068 MsG0480022797.01.T01:CDS 35.0%
GCTAAGATTGCAAAAGAAAG+AGG - Chr4:76778555-76778574 None:intergenic 35.0%
TATAACTGCTTTGTGGCATT+TGG + Chr4:76778977-76778996 MsG0480022797.01.T01:CDS 35.0%
TCTTAAGAGCAGCATTCAAA+TGG + Chr4:76779355-76779374 MsG0480022797.01.T01:CDS 35.0%
TTAAGATGTTAGGGATGTTG+AGG - Chr4:76778464-76778483 None:intergenic 35.0%
TTATGAGTTGTTGAAGGGTA+GGG + Chr4:76779055-76779074 MsG0480022797.01.T01:CDS 35.0%
TTATTTGCGCGGTTATTAGT+GGG + Chr4:76779114-76779133 MsG0480022797.01.T01:CDS 35.0%
TTGAGGAATTGAAGTTCTTC+AGG - Chr4:76778447-76778466 None:intergenic 35.0%
! AAATTAAGGGGTGTGTGAAA+AGG - Chr4:76778584-76778603 None:intergenic 35.0%
! AATTAAGGGGTGTGTGAAAA+GGG - Chr4:76778583-76778602 None:intergenic 35.0%
!! TGTTCTTTGTTTGGTTCTGA+TGG + Chr4:76778869-76778888 MsG0480022797.01.T01:CDS 35.0%
!! TTTCTAGGATTGTCATTGGT+GGG + Chr4:76779183-76779202 MsG0480022797.01.T01:CDS 35.0%
!!! GTGTTTTCTAGGATTGTCAT+TGG + Chr4:76779179-76779198 MsG0480022797.01.T01:CDS 35.0%
!!! TTTTCTAGGATTGTCATTGG+TGG + Chr4:76779182-76779201 MsG0480022797.01.T01:CDS 35.0%
AGAAAGCGAAGAGGAAAATG+AGG - Chr4:76778686-76778705 None:intergenic 40.0%
CTTATTTGCGCGGTTATTAG+TGG + Chr4:76779113-76779132 MsG0480022797.01.T01:CDS 40.0%
GCTTTACATGATCATCTTGG+TGG + Chr4:76779311-76779330 MsG0480022797.01.T01:CDS 40.0%
GTTATGAGTTGTTGAAGGGT+AGG + Chr4:76779054-76779073 MsG0480022797.01.T01:CDS 40.0%
TATTAGTGGGATGTTTAGCG+TGG + Chr4:76779127-76779146 MsG0480022797.01.T01:CDS 40.0%
TCTGTGCTTGAACCTCTTTA+TGG + Chr4:76779011-76779030 MsG0480022797.01.T01:CDS 40.0%
! AGCAGTTGATACTGACAACT+CGG + Chr4:76778896-76778915 MsG0480022797.01.T01:CDS 40.0%
! ATGGTTATGGCGTGTTTTCT+AGG + Chr4:76779168-76779187 MsG0480022797.01.T01:CDS 40.0%
! GGTTAGATATGCTGCACTTT+TGG + Chr4:76779076-76779095 MsG0480022797.01.T01:CDS 40.0%
! TAAGAGGCACATATTCTCCA+AGG - Chr4:76779340-76779359 None:intergenic 40.0%
! TGTGAATTTGGTTGGGTTGT+TGG + Chr4:76779229-76779248 MsG0480022797.01.T01:CDS 40.0%
!!! TATGCTGCACTTTTGGTTTG+CGG + Chr4:76779083-76779102 MsG0480022797.01.T01:CDS 40.0%
AAAGGAGAGAGAAAGCGAAG+AGG - Chr4:76778695-76778714 None:intergenic 45.0%
AGAAGCGACGGTGAAAACAA+CGG - Chr4:76778727-76778746 None:intergenic 45.0%
CAATCATGAGAGGATTGTGC+AGG - Chr4:76778633-76778652 None:intergenic 45.0%
CGGGTATTTGCTTATTTGCG+CGG + Chr4:76779103-76779122 MsG0480022797.01.T01:CDS 45.0%
CTGCACAATCCTCTCATGAT+TGG + Chr4:76778631-76778650 MsG0480022797.01.T01:CDS 45.0%
GAGAAGAGTCCAATCATGAG+AGG - Chr4:76778643-76778662 None:intergenic 45.0%
GATTGTGCAGGATCAGTGAA+AGG - Chr4:76778621-76778640 None:intergenic 45.0%
TTCTCTCTACACCTCAAAGC+CGG + Chr4:76778743-76778762 MsG0480022797.01.T01:CDS 45.0%
TTTAGCGTGGTTGTGGTACA+TGG + Chr4:76779140-76779159 MsG0480022797.01.T01:CDS 45.0%
!! GATTGTCATTGGTGGGTTCT+TGG + Chr4:76779190-76779209 MsG0480022797.01.T01:CDS 45.0%
!! GTACATGGTGGTGATGGTTA+TGG + Chr4:76779155-76779174 MsG0480022797.01.T01:CDS 45.0%
!! TGAAAAAGACACCGGCTTTG+AGG - Chr4:76778757-76778776 None:intergenic 45.0%
!!! ATGCTGCACTTTTGGTTTGC+GGG + Chr4:76779084-76779103 MsG0480022797.01.T01:CDS 45.0%
TGAGGTGTAGAGAGAAGCGA+CGG - Chr4:76778739-76778758 None:intergenic 50.0%
TGGGATGTTTAGCGTGGTTG+TGG + Chr4:76779133-76779152 MsG0480022797.01.T01:CDS 50.0%
! GATCATCTTGGTGGCTACCT+TGG + Chr4:76779320-76779339 MsG0480022797.01.T01:CDS 50.0%
! GTTGTGGTACATGGTGGTGA+TGG + Chr4:76779149-76779168 MsG0480022797.01.T01:CDS 50.0%
!! GTCATTGGTGGGTTCTTGGT+TGG + Chr4:76779194-76779213 MsG0480022797.01.T01:CDS 50.0%
AAACAACGGCAGCGGTGGAA+AGG - Chr4:76778713-76778732 None:intergenic 55.0%
AGCGTGGTTGTGGTACATGG+TGG + Chr4:76779143-76779162 MsG0480022797.01.T01:CDS 55.0%
GACGGTGAAAACAACGGCAG+CGG - Chr4:76778721-76778740 None:intergenic 55.0%
GGTGAAAACAACGGCAGCGG+TGG - Chr4:76778718-76778737 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 76778432 76779394 76778432 ID=MsG0480022797.01;Name=MsG0480022797.01
Chr4 mRNA 76778432 76779394 76778432 ID=MsG0480022797.01.T01;Parent=MsG0480022797.01;Name=MsG0480022797.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|320
Chr4 exon 76778432 76779394 76778432 ID=MsG0480022797.01.T01:exon:19822;Parent=MsG0480022797.01.T01
Chr4 CDS 76778432 76779394 76778432 ID=MsG0480022797.01.T01:cds;Parent=MsG0480022797.01.T01
Gene Sequence

>MsG0480022797.01.T01

ATGGATCTAGCTCCTGAAGAACTTCAATTCCTCAACATCCCTAACATCTTAAAAGAATCAATTTCAATCCCAAAAATTTCCCCTAAAACCTTCTATTTAATTACCCTAACCCTAATTTTCCCTCTTTCTTTTGCAATCTTAGCTCATTCCCTTTTCACACACCCCTTAATTTCTCAGCTTCAATCTCCTTTCACTGATCCTGCACAATCCTCTCATGATTGGACTCTTCTCCTTATCATTCAGTTTTTCTACCTCATTTTCCTCTTCGCTTTCTCTCTCCTTTCCACCGCTGCCGTTGTTTTCACCGTCGCTTCTCTCTACACCTCAAAGCCGGTGTCTTTTTCAAACACCATTTCAGCAATTCCTAAAGTTTTTAAGCGTTTGTTTATTACTTTCTTATGGGTCACTCTACTTATGATTTGTTACAATTTCGTCTTTGTTCTTTGTTTGGTTCTGATGGTTGTAGCAGTTGATACTGACAACTCGGTTTTGTTGTTTTTCTCTGTTGTTTTGATTTTTGTGTTGTTTCTTGTTGTTCATGTTTATATAACTGCTTTGTGGCATTTGGCTAGTGTTGTGTCTGTGCTTGAACCTCTTTATGGTTTTGCTGCTATGAAGAAGAGTTATGAGTTGTTGAAGGGTAGGGTTAGATATGCTGCACTTTTGGTTTGCGGGTATTTGCTTATTTGCGCGGTTATTAGTGGGATGTTTAGCGTGGTTGTGGTACATGGTGGTGATGGTTATGGCGTGTTTTCTAGGATTGTCATTGGTGGGTTCTTGGTTGGTGTTCTTGTCATTGTGAATTTGGTTGGGTTGTTGGTGCAGAGTGTGTTTTACTATGTTTGCAAGAGTTATCATCATCAAGAGATTGATAAGAGTGCTTTACATGATCATCTTGGTGGCTACCTTGGAGAATATGTGCCTCTTAAGAGCAGCATTCAAATGGAAAATTTGGATATATGA

Protein sequence

>MsG0480022797.01.T01

MDLAPEELQFLNIPNILKESISIPKISPKTFYLITLTLIFPLSFAILAHSLFTHPLISQLQSPFTDPAQSSHDWTLLLIIQFFYLIFLFAFSLLSTAAVVFTVASLYTSKPVSFSNTISAIPKVFKRLFITFLWVTLLMICYNFVFVLCLVLMVVAVDTDNSVLLFFSVVLIFVLFLVVHVYITALWHLASVVSVLEPLYGFAAMKKSYELLKGRVRYAALLVCGYLLICAVISGMFSVVVVHGGDGYGVFSRIVIGGFLVGVLVIVNLVGLLVQSVFYYVCKSYHHQEIDKSALHDHLGGYLGEYVPLKSSIQMENLDI*