Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043715.01.T01 | XP_003627885.2 | 95.42 | 262 | 6 | 1 | 1 | 262 | 84 | 339 | 1.97E-174 | 494 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043715.01.T01 | E4NKF8 | 29.108 | 213 | 133 | 7 | 5 | 214 | 486 | 683 | 4.42E-14 | 75.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043715.01.T01 | G7LGS1 | 95.420 | 262 | 6 | 1 | 1 | 262 | 84 | 339 | 9.39e-175 | 494 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048692.01 | MsG0880043715.01 | -0.804865 | 1.759828e-49 | 9.174846e-47 |
| MsG0080048848.01 | MsG0880043715.01 | 0.804369 | 2.233362e-49 | 1.149483e-46 |
| MsG0180000019.01 | MsG0880043715.01 | 0.839407 | 1.614287e-57 | 2.227339e-54 |
| MsG0180000089.01 | MsG0880043715.01 | 0.805925 | 1.054743e-49 | 5.650480e-47 |
| MsG0180000222.01 | MsG0880043715.01 | 0.816381 | 5.695286e-52 | 4.029285e-49 |
| MsG0180000337.01 | MsG0880043715.01 | 0.839986 | 1.140829e-57 | 1.602603e-54 |
| MsG0180001050.01 | MsG0880043715.01 | 0.833121 | 6.395929e-56 | 7.297084e-53 |
| MsG0180001053.01 | MsG0880043715.01 | 0.818164 | 2.260802e-52 | 1.679542e-49 |
| MsG0180002214.01 | MsG0880043715.01 | -0.811908 | 5.533584e-51 | 3.468039e-48 |
| MsG0180002546.01 | MsG0880043715.01 | -0.838485 | 2.796976e-57 | 3.751520e-54 |
| MsG0180004079.01 | MsG0880043715.01 | 0.809741 | 1.628502e-50 | 9.639249e-48 |
| MsG0180004110.01 | MsG0880043715.01 | 0.863362 | 2.628187e-64 | 7.946850e-61 |
| MsG0180004562.01 | MsG0880043715.01 | 0.827391 | 1.605196e-54 | 1.547770e-51 |
| MsG0180004752.01 | MsG0880043715.01 | 0.874543 | 6.221502e-68 | 2.811172e-64 |
| MsG0180004761.01 | MsG0880043715.01 | -0.809479 | 1.853815e-50 | 1.089612e-47 |
| MsG0180004867.01 | MsG0880043715.01 | 0.843540 | 1.315834e-58 | 2.065349e-55 |
| MsG0180005172.01 | MsG0880043715.01 | 0.801361 | 9.337086e-49 | 4.450439e-46 |
| MsG0180005331.01 | MsG0880043715.01 | 0.806710 | 7.207768e-50 | 3.939429e-47 |
| MsG0180006012.01 | MsG0880043715.01 | 0.892795 | 1.141529e-74 | 1.058967e-70 |
| MsG0780040313.01 | MsG0880043715.01 | 0.805641 | 1.210433e-49 | 6.437305e-47 |
| MsG0780041380.01 | MsG0880043715.01 | 0.825196 | 5.347467e-54 | 4.840957e-51 |
| MsG0880041985.01 | MsG0880043715.01 | 0.854997 | 8.475944e-62 | 1.928298e-58 |
| MsG0880042368.01 | MsG0880043715.01 | 0.813241 | 2.827158e-51 | 1.836249e-48 |
| MsG0880042843.01 | MsG0880043715.01 | 0.812540 | 4.027236e-51 | 2.567647e-48 |
| MsG0880043285.01 | MsG0880043715.01 | 0.838097 | 3.521342e-57 | 4.667283e-54 |
| MsG0880043685.01 | MsG0880043715.01 | 0.906769 | 1.041477e-80 | 1.790337e-76 |
| MsG0880043715.01 | MsG0880044100.01 | 0.818409 | 1.990043e-52 | 1.488556e-49 |
| MsG0880043715.01 | MsG0880044570.01 | 0.816677 | 4.887409e-52 | 3.485840e-49 |
| MsG0880043715.01 | MsG0880044669.01 | 0.809621 | 1.728614e-50 | 1.019946e-47 |
| MsG0880043715.01 | MsG0880045277.01 | 0.844535 | 7.118451e-59 | 1.152757e-55 |
| MsG0880043715.01 | MsG0880045819.01 | 0.800355 | 1.498632e-48 | 6.963466e-46 |
| MsG0880043715.01 | MsG0880045861.01 | 0.810335 | 1.213181e-50 | 7.292814e-48 |
| MsG0880043715.01 | MsG0880045907.01 | 0.828170 | 1.043400e-54 | 1.029046e-51 |
| MsG0880043715.01 | MsG0880046099.01 | -0.820098 | 8.207420e-53 | 6.433956e-50 |
| MsG0880043715.01 | MsG0880046226.01 | 0.836900 | 7.133945e-57 | 9.116331e-54 |
| MsG0880043715.01 | MsG0880046251.01 | -0.842905 | 1.942902e-58 | 2.989072e-55 |
| MsG0880043715.01 | MsG0880047081.01 | 0.836525 | 8.894511e-57 | 1.123750e-53 |
| MsG0880043715.01 | MsG0880047233.01 | 0.816172 | 6.341282e-52 | 4.460844e-49 |
| MsG0880043715.01 | MsG0880047256.01 | 0.805897 | 1.069424e-49 | 5.724991e-47 |
| MsG0880043715.01 | MsG0880047486.01 | 0.825703 | 4.056538e-54 | 3.725566e-51 |
| MsG0880043715.01 | MsG0880047491.01 | 0.831967 | 1.236236e-55 | 1.362770e-52 |
| MsG0880043715.01 | MsG0880047531.01 | 0.842937 | 1.906013e-58 | 2.935011e-55 |
| MsG0280011041.01 | MsG0880043715.01 | 0.813075 | 3.074903e-51 | 1.988206e-48 |
| MsG0280011053.01 | MsG0880043715.01 | 0.825255 | 5.179439e-54 | 4.696549e-51 |
| MsG0280011152.01 | MsG0880043715.01 | 0.854573 | 1.124984e-61 | 2.523659e-58 |
| MsG0280011288.01 | MsG0880043715.01 | 0.811709 | 6.113065e-51 | 3.811062e-48 |
| MsG0280011473.01 | MsG0880043715.01 | 0.810708 | 1.007954e-50 | 6.119969e-48 |
| MsG0380011613.01 | MsG0880043715.01 | 0.801792 | 7.616598e-49 | 3.670132e-46 |
| MsG0380013940.01 | MsG0880043715.01 | -0.807694 | 4.459231e-50 | 2.500235e-47 |
| MsG0380013945.01 | MsG0880043715.01 | -0.808003 | 3.833287e-50 | 2.166880e-47 |
| MsG0380013953.01 | MsG0880043715.01 | -0.817821 | 2.703206e-52 | 1.989421e-49 |
| MsG0380013957.01 | MsG0880043715.01 | -0.829759 | 4.300684e-55 | 4.443665e-52 |
| MsG0380013967.01 | MsG0880043715.01 | -0.827951 | 1.178197e-54 | 1.154574e-51 |
| MsG0380013977.01 | MsG0880043715.01 | -0.819914 | 9.042346e-53 | 7.051701e-50 |
| MsG0380014006.01 | MsG0880043715.01 | -0.822725 | 2.031243e-53 | 1.714126e-50 |
| MsG0380014032.01 | MsG0880043715.01 | -0.808738 | 2.672820e-50 | 1.540138e-47 |
| MsG0380014920.01 | MsG0880043715.01 | 0.828744 | 7.582432e-55 | 7.603206e-52 |
| MsG0480018496.01 | MsG0880043715.01 | 0.803219 | 3.870353e-49 | 1.933989e-46 |
| MsG0480018500.01 | MsG0880043715.01 | 0.846531 | 2.049159e-59 | 3.537778e-56 |
| MsG0480019038.01 | MsG0880043715.01 | 0.826422 | 2.736975e-54 | 2.565703e-51 |
| MsG0480019880.01 | MsG0880043715.01 | 0.865653 | 5.050668e-65 | 1.656159e-61 |
| MsG0480020444.01 | MsG0880043715.01 | 0.849313 | 3.503831e-60 | 6.615896e-57 |
| MsG0480021135.01 | MsG0880043715.01 | 0.811685 | 6.185595e-51 | 3.853816e-48 |
| MsG0480022125.01 | MsG0880043715.01 | 0.864745 | 9.748574e-65 | 3.094159e-61 |
| MsG0480022486.01 | MsG0880043715.01 | 0.808980 | 2.372379e-50 | 1.375906e-47 |
| MsG0480022520.01 | MsG0880043715.01 | 0.831118 | 2.000633e-55 | 2.150417e-52 |
| MsG0480022760.01 | MsG0880043715.01 | 0.841358 | 4.988321e-58 | 7.311342e-55 |
| MsG0480022797.01 | MsG0880043715.01 | 0.829607 | 4.683474e-55 | 4.817123e-52 |
| MsG0480022974.01 | MsG0880043715.01 | 0.816343 | 5.806014e-52 | 4.103685e-49 |
| MsG0480023318.01 | MsG0880043715.01 | 0.831417 | 1.689480e-55 | 1.832183e-52 |
| MsG0580024871.01 | MsG0880043715.01 | 0.828463 | 8.864899e-55 | 8.818042e-52 |
| MsG0580024999.01 | MsG0880043715.01 | 0.865922 | 4.153599e-65 | 1.374734e-61 |
| MsG0580025876.01 | MsG0880043715.01 | 0.801148 | 1.032310e-48 | 4.894108e-46 |
| MsG0380015010.01 | MsG0880043715.01 | 0.816412 | 5.603452e-52 | 3.967626e-49 |
| MsG0380015245.01 | MsG0880043715.01 | 0.822705 | 2.053296e-53 | 1.731736e-50 |
| MsG0380016367.01 | MsG0880043715.01 | -0.812694 | 3.727647e-51 | 2.386142e-48 |
| MsG0380016800.01 | MsG0880043715.01 | 0.807665 | 4.524815e-50 | 2.535052e-47 |
| MsG0380016926.01 | MsG0880043715.01 | 0.815275 | 1.004691e-51 | 6.895954e-49 |
| MsG0380017766.01 | MsG0880043715.01 | 0.805187 | 1.506855e-49 | 7.920737e-47 |
| MsG0580027073.01 | MsG0880043715.01 | 0.807387 | 5.181133e-50 | 2.882052e-47 |
| MsG0580027227.01 | MsG0880043715.01 | 0.845032 | 5.230218e-59 | 8.603751e-56 |
| MsG0580028051.01 | MsG0880043715.01 | 0.800409 | 1.460867e-48 | 6.797404e-46 |
| MsG0580028355.01 | MsG0880043715.01 | 0.850973 | 1.201004e-60 | 2.394278e-57 |
| MsG0580028434.01 | MsG0880043715.01 | 0.811710 | 6.110088e-51 | 3.809285e-48 |
| MsG0580028560.01 | MsG0880043715.01 | 0.840089 | 1.072433e-57 | 1.511382e-54 |
| MsG0580028999.01 | MsG0880043715.01 | 0.809962 | 1.460212e-50 | 8.693119e-48 |
| MsG0580029583.01 | MsG0880043715.01 | 0.817796 | 2.738730e-52 | 2.014200e-49 |
| MsG0580029798.01 | MsG0880043715.01 | 0.830694 | 2.542452e-55 | 2.699021e-52 |
| MsG0580029901.01 | MsG0880043715.01 | 0.844285 | 8.311155e-59 | 1.335320e-55 |
| MsG0580029906.01 | MsG0880043715.01 | 0.811711 | 6.106235e-51 | 3.806978e-48 |
| MsG0580029930.01 | MsG0880043715.01 | 0.834122 | 3.597620e-56 | 4.228918e-53 |
| MsG0280006475.01 | MsG0880043715.01 | -0.821614 | 3.676159e-53 | 3.005438e-50 |
| MsG0280007272.01 | MsG0880043715.01 | 0.809996 | 1.435361e-50 | 8.553105e-48 |
| MsG0280007369.01 | MsG0880043715.01 | 0.831756 | 1.393488e-55 | 1.526713e-52 |
| MsG0280007391.01 | MsG0880043715.01 | 0.834818 | 2.404770e-56 | 2.886360e-53 |
| MsG0280007751.01 | MsG0880043715.01 | 0.810763 | 9.803930e-51 | 5.961361e-48 |
| MsG0280007840.01 | MsG0880043715.01 | 0.853989 | 1.659417e-61 | 3.651823e-58 |
| MsG0280009394.01 | MsG0880043715.01 | 0.859257 | 4.686193e-63 | 1.229854e-59 |
| MsG0280009899.01 | MsG0880043715.01 | 0.800062 | 1.718898e-48 | 7.927657e-46 |
| MsG0280009971.01 | MsG0880043715.01 | 0.812074 | 5.091682e-51 | 3.205640e-48 |
| MsG0680035741.01 | MsG0880043715.01 | 0.849993 | 2.263366e-60 | 4.369229e-57 |
| MsG0680035777.01 | MsG0880043715.01 | 0.828166 | 1.045589e-54 | 1.031090e-51 |
| MsG0680035842.01 | MsG0880043715.01 | 0.801316 | 9.538797e-49 | 4.541388e-46 |
| MsG0780037599.01 | MsG0880043715.01 | 0.872600 | 2.808655e-67 | 1.182350e-63 |
| MsG0780038123.01 | MsG0880043715.01 | 0.868899 | 4.633509e-66 | 1.704575e-62 |
| MsG0780038135.01 | MsG0880043715.01 | 0.835284 | 1.835576e-56 | 2.234513e-53 |
| MsG0780039450.01 | MsG0880043715.01 | 0.831544 | 1.572019e-55 | 1.711415e-52 |
| MsG0780039472.01 | MsG0880043715.01 | 0.850995 | 1.183790e-60 | 2.361722e-57 |
| MsG0780039877.01 | MsG0880043715.01 | 0.830325 | 3.129929e-55 | 3.286800e-52 |
| MsG0780040144.01 | MsG0880043715.01 | 0.884911 | 1.279676e-71 | 8.601445e-68 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0780041704.01 | MsG0880043715.01 | PPI |
| MsG0880043715.01 | MsG0180004421.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043715.01.T01 | MTR_8g039560 | 95.420 | 262 | 6 | 1 | 1 | 262 | 84 | 339 | 2.38e-178 | 494 |
| MsG0880043715.01.T01 | MTR_5g083030 | 29.108 | 213 | 133 | 7 | 5 | 214 | 486 | 683 | 4.30e-15 | 75.1 |
| MsG0880043715.01.T01 | MTR_2g018010 | 31.579 | 190 | 119 | 5 | 25 | 214 | 495 | 673 | 1.37e-12 | 67.8 |
| MsG0880043715.01.T01 | MTR_4g485520 | 29.412 | 187 | 119 | 7 | 25 | 211 | 470 | 643 | 3.05e-11 | 63.5 |
| MsG0880043715.01.T01 | MTR_4g485520 | 29.412 | 187 | 119 | 7 | 25 | 211 | 302 | 475 | 3.68e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043715.01.T01 | AT1G08315 | 57.534 | 219 | 88 | 2 | 1 | 215 | 85 | 302 | 1.44e-81 | 248 |
| MsG0880043715.01.T01 | AT2G23140 | 30.435 | 207 | 126 | 7 | 5 | 211 | 435 | 623 | 3.45e-14 | 72.4 |
| MsG0880043715.01.T01 | AT2G23140 | 30.435 | 207 | 126 | 7 | 5 | 211 | 435 | 623 | 3.45e-14 | 72.4 |
| MsG0880043715.01.T01 | AT2G23140 | 30.918 | 207 | 125 | 7 | 5 | 211 | 624 | 812 | 7.90e-14 | 71.6 |
| MsG0880043715.01.T01 | AT2G23140 | 30.918 | 207 | 125 | 7 | 5 | 211 | 624 | 812 | 7.90e-14 | 71.6 |
| MsG0880043715.01.T01 | AT2G23140 | 30.918 | 207 | 125 | 7 | 5 | 211 | 627 | 815 | 8.21e-14 | 71.6 |
| MsG0880043715.01.T01 | AT1G23030 | 28.421 | 190 | 124 | 5 | 25 | 214 | 430 | 607 | 4.91e-11 | 63.2 |
| MsG0880043715.01.T01 | AT1G29340 | 28.980 | 245 | 154 | 8 | 8 | 249 | 490 | 717 | 6.21e-11 | 62.8 |
Find 69 sgRNAs with CRISPR-Local
Find 101 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAATGGTGGTTCGATATT+TGG | 0.196098 | 8:+29568788 | MsG0880043715.01.T01:exon |
| GAGGCGTTTAAGAAGGTTTC+TGG | 0.219872 | 8:+29568396 | MsG0880043715.01.T01:CDS |
| TTTCTTCGGTGGATGATTAT+CGG | 0.308765 | 8:+29567567 | MsG0880043715.01.T01:CDS |
| TGAATGGTGGTTCGTCGTTT+TGG | 0.311645 | 8:+29568845 | MsG0880043715.01.T01:three_prime_UTR |
| ACATCTCTGATTTAGGTTGC+TGG | 0.315701 | 8:+29568357 | MsG0880043715.01.T01:intron |
| AACGAATCTTTCACCGTCCT+TGG | 0.340118 | 8:-29567662 | None:intergenic |
| ATTGCTGATGTTAAAGATAA+AGG | 0.343931 | 8:+29568576 | MsG0880043715.01.T01:CDS |
| TTGAGTAGTGATTCGTCGTT+TGG | 0.357523 | 8:+29568732 | MsG0880043715.01.T01:CDS |
| GATTCACAGCCTTCTTTCTT+CGG | 0.371042 | 8:+29567553 | MsG0880043715.01.T01:CDS |
| GGTTCAGTTTGGTGTTGTTC+CGG | 0.382972 | 8:+29567733 | MsG0880043715.01.T01:CDS |
| AAAGATAAAGGAAGTGTTAA+GGG | 0.391288 | 8:+29568588 | MsG0880043715.01.T01:CDS |
| TTTCTACTGGTTCGAGTATG+AGG | 0.408987 | 8:+29568448 | MsG0880043715.01.T01:CDS |
| TTTAAGAAGGTTTCTGGTGT+TGG | 0.413108 | 8:+29568402 | MsG0880043715.01.T01:CDS |
| GCGCGGTGTTCTCGACGCGC+TGG | 0.421005 | 8:+29567466 | MsG0880043715.01.T01:CDS |
| TAAAGATAAAGGAAGTGTTA+AGG | 0.422699 | 8:+29568587 | MsG0880043715.01.T01:CDS |
| CGCGGTGGCGTTTGGTGCGA+TGG | 0.437208 | 8:+29568548 | MsG0880043715.01.T01:CDS |
| GTAAGAGACGCGGTGGCGTT+TGG | 0.449671 | 8:+29568540 | MsG0880043715.01.T01:CDS |
| GATGATTATCGGACTGTTGT+TGG | 0.454158 | 8:+29567578 | MsG0880043715.01.T01:CDS |
| AAGTGTTAAGGGGCAGAGTA+AGG | 0.459646 | 8:+29568599 | MsG0880043715.01.T01:CDS |
| AAGTGTTGAGGCGTTTAAGA+AGG | 0.460925 | 8:+29568389 | MsG0880043715.01.T01:CDS |
| GGATTGAACAGCAGCGGCGG+CGG | 0.470726 | 8:-29567519 | None:intergenic |
| ATTGTTAATGATGGTAGAGT+AGG | 0.470952 | 8:+29567770 | MsG0880043715.01.T01:CDS |
| TGCTTCCGCGGTTATTGCAC+AGG | 0.471008 | 8:+29567805 | MsG0880043715.01.T01:CDS |
| GATCTGCTCGATCTTTCTAC+TGG | 0.482484 | 8:+29568435 | MsG0880043715.01.T01:CDS |
| TTGTTAATGATGGTAGAGTA+GGG | 0.494346 | 8:+29567771 | MsG0880043715.01.T01:CDS |
| CGTGCTACGATGGTTCAGTT+TGG | 0.504494 | 8:+29567722 | MsG0880043715.01.T01:CDS |
| CTGGTTCGAGTATGAGGACG+AGG | 0.504707 | 8:+29568454 | MsG0880043715.01.T01:CDS |
| GTGGCGTTTGGTGCGATGGA+TGG | 0.506730 | 8:+29568552 | MsG0880043715.01.T01:CDS |
| GGCGGATTGAACAGCAGCGG+CGG | 0.510709 | 8:-29567522 | None:intergenic |
| TGTGGTGGAGATGTGGTGGC+TGG | 0.510838 | 8:+29568513 | MsG0880043715.01.T01:CDS |
| TTGAACAGCAGCGGCGGCGG+AGG | 0.511456 | 8:-29567516 | None:intergenic |
| GTTCGTAGTCACGGTAATGT+TGG | 0.512410 | 8:+29568708 | MsG0880043715.01.T01:CDS |
| TCACAGCCTTCTTTCTTCGG+TGG | 0.513803 | 8:+29567556 | MsG0880043715.01.T01:CDS |
| GGTTTCTGGTGTTGGAGTGT+TGG | 0.526980 | 8:+29568410 | MsG0880043715.01.T01:CDS |
| TTCTGGTGTTGGAGTGTTGG+CGG | 0.530110 | 8:+29568413 | MsG0880043715.01.T01:CDS |
| TCCGTTGAATCGTGCTACGA+TGG | 0.531126 | 8:+29567712 | MsG0880043715.01.T01:CDS |
| GAATCTTTCACCGTCCTTGG+CGG | 0.531492 | 8:-29567659 | None:intergenic |
| AATCATCCACCGAAGAAAGA+AGG | 0.536679 | 8:-29567562 | None:intergenic |
| TCTTTCATGAATGAAGATAG+TGG | 0.538879 | 8:+29568756 | MsG0880043715.01.T01:CDS |
| GACGAGGGAAAATGCGGTGT+CGG | 0.541817 | 8:+29568470 | MsG0880043715.01.T01:CDS |
| CACGGTGATGTTCGTAGTGA+TGG | 0.549767 | 8:+29568681 | MsG0880043715.01.T01:CDS |
| TGTGAGATGTGGTGGAGATG+TGG | 0.560397 | 8:+29568506 | MsG0880043715.01.T01:CDS |
| ACCATCGTAGCACGATTCAA+CGG | 0.561289 | 8:-29567713 | None:intergenic |
| CCGTCCTTGGCGGTGAAGAA+CGG | 0.564287 | 8:-29567649 | None:intergenic |
| GATTTAGGTTGCTGGTTGCG+AGG | 0.564684 | 8:+29568365 | MsG0880043715.01.T01:intron |
| CTTAATCTTGTGAGATGTGG+TGG | 0.564928 | 8:+29568498 | MsG0880043715.01.T01:CDS |
| CACGGTGATGTTCGTAGTCA+CGG | 0.566505 | 8:+29568663 | MsG0880043715.01.T01:CDS |
| TTGCTTAATCTTGTGAGATG+TGG | 0.567457 | 8:+29568495 | MsG0880043715.01.T01:CDS |
| CGCGTCGAGAACACCGCGCG+TGG | 0.576042 | 8:-29567462 | None:intergenic |
| GCTGTGAATCGTAGCGGCGG+CGG | 0.577574 | 8:-29567540 | None:intergenic |
| GTCACCGTTCTTCACCGCCA+AGG | 0.589598 | 8:+29567645 | MsG0880043715.01.T01:CDS |
| GAGTTGATGAGAGTGATGGT+TGG | 0.595956 | 8:+29568627 | MsG0880043715.01.T01:CDS |
| GTTGGTGATGTTCGTAGTCA+CGG | 0.603776 | 8:+29568645 | MsG0880043715.01.T01:CDS |
| AAGATAAAGGAAGTGTTAAG+GGG | 0.604994 | 8:+29568589 | MsG0880043715.01.T01:CDS |
| CCGTTCTTCACCGCCAAGGA+CGG | 0.608476 | 8:+29567649 | MsG0880043715.01.T01:CDS |
| GAGATGTGGTGGAGATGTGG+TGG | 0.610433 | 8:+29568509 | MsG0880043715.01.T01:CDS |
| TATGAGGACGAGGGAAAATG+CGG | 0.612956 | 8:+29568464 | MsG0880043715.01.T01:CDS |
| GGCGGCGGATTGAACAGCAG+CGG | 0.615428 | 8:-29567525 | None:intergenic |
| GATGGTGATGTTCGTAGTCA+CGG | 0.622057 | 8:+29568699 | MsG0880043715.01.T01:CDS |
| GATTGTTGAAGATGCTTCCG+CGG | 0.638020 | 8:+29567793 | MsG0880043715.01.T01:CDS |
| TGGAGATGTAAGAGACGCGG+TGG | 0.667404 | 8:+29568533 | MsG0880043715.01.T01:CDS |
| AAGGCTGTGAATCGTAGCGG+CGG | 0.667512 | 8:-29567543 | None:intergenic |
| TGGTTGCGAGGAAAGTGTTG+AGG | 0.674105 | 8:+29568377 | MsG0880043715.01.T01:CDS |
| ACAATCAACGAAAACAACGC+CGG | 0.685893 | 8:-29567752 | None:intergenic |
| GAACCTATCATGTCCACGCG+CGG | 0.689698 | 8:+29567449 | None:intergenic |
| AAGAAGGCTGTGAATCGTAG+CGG | 0.720130 | 8:-29567546 | None:intergenic |
| TGGTTCGAGTATGAGGACGA+GGG | 0.725606 | 8:+29568455 | MsG0880043715.01.T01:CDS |
| GGCTGGAGATGTAAGAGACG+CGG | 0.727136 | 8:+29568530 | MsG0880043715.01.T01:CDS |
| AATAACCTGTGCAATAACCG+CGG | 0.737571 | 8:-29567810 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAAGTTGAATTAAATTAA+GGG | + | Chr8:29567881-29567900 | MsG0880043715.01.T01:intron | 15.0% |
| !! | CTATCTTATTATATTACTTA+GGG | - | Chr8:29568284-29568303 | None:intergenic | 15.0% |
| !!! | TAAGAGTTAATTATTTTAAC+TGG | - | Chr8:29568307-29568326 | None:intergenic | 15.0% |
| !! | AAAAAGCTCATTAATTGAAA+AGG | + | Chr8:29569019-29569038 | MsG0880043715.01.T01:three_prime_UTR | 20.0% |
| !! | GAAGAAGTTGAATTAAATTA+AGG | + | Chr8:29567880-29567899 | MsG0880043715.01.T01:intron | 20.0% |
| !! | GCTATCTTATTATATTACTT+AGG | - | Chr8:29568285-29568304 | None:intergenic | 20.0% |
| !! | TATTCATAAGTTATGAAACA+CGG | - | Chr8:29568196-29568215 | None:intergenic | 20.0% |
| ! | ATTGCTGATGTTAAAGATAA+AGG | + | Chr8:29568576-29568595 | MsG0880043715.01.T01:CDS | 25.0% |
| ! | TTCTGTGTAATTATATGTGT+CGG | + | Chr8:29568104-29568123 | MsG0880043715.01.T01:intron | 25.0% |
| ! | TTTCAAATTATTAGCAGTGT+TGG | - | Chr8:29568073-29568092 | None:intergenic | 25.0% |
| !! | AACTTTTACATCTCTGATTT+AGG | + | Chr8:29568350-29568369 | MsG0880043715.01.T01:intron | 25.0% |
| !! | TTTCATAAACTAGCTTGATA+AGG | + | Chr8:29567993-29568012 | MsG0880043715.01.T01:intron | 25.0% |
| !!! | AAAGATAAAGGAAGTGTTAA+GGG | + | Chr8:29568588-29568607 | MsG0880043715.01.T01:CDS | 25.0% |
| !!! | TAAAGATAAAGGAAGTGTTA+AGG | + | Chr8:29568587-29568606 | MsG0880043715.01.T01:CDS | 25.0% |
| !!! | TTTTCGTTGATTGTTAATGA+TGG | + | Chr8:29567761-29567780 | MsG0880043715.01.T01:CDS | 25.0% |
| ATTGTTAATGATGGTAGAGT+AGG | + | Chr8:29567770-29567789 | MsG0880043715.01.T01:CDS | 30.0% | |
| TATATTACTTAGGGTCTGTT+TGG | - | Chr8:29568275-29568294 | None:intergenic | 30.0% | |
| TCTTTCATGAATGAAGATAG+TGG | + | Chr8:29568756-29568775 | MsG0880043715.01.T01:CDS | 30.0% | |
| TTGTTAATGATGGTAGAGTA+GGG | + | Chr8:29567771-29567790 | MsG0880043715.01.T01:CDS | 30.0% | |
| !! | AAGATAAAGGAAGTGTTAAG+GGG | + | Chr8:29568589-29568608 | MsG0880043715.01.T01:CDS | 30.0% |
| !! | TAGTGTTACATCGTTTTGAA+TGG | + | Chr8:29568829-29568848 | MsG0880043715.01.T01:three_prime_UTR | 30.0% |
| AAGATTAGACGTCACATTGT+CGG | - | Chr8:29568165-29568184 | None:intergenic | 35.0% | |
| ACAATGTGACGTCTAATCTT+AGG | + | Chr8:29568165-29568184 | MsG0880043715.01.T01:intron | 35.0% | |
| CTTGATAAGGTTATGAAGCA+CGG | + | Chr8:29568006-29568025 | MsG0880043715.01.T01:intron | 35.0% | |
| TTGAATGGTGGTTCGATATT+TGG | + | Chr8:29568788-29568807 | MsG0880043715.01.T01:exon | 35.0% | |
| TTGCTTAATCTTGTGAGATG+TGG | + | Chr8:29568495-29568514 | MsG0880043715.01.T01:CDS | 35.0% | |
| TTTCTTCGGTGGATGATTAT+CGG | + | Chr8:29567567-29567586 | MsG0880043715.01.T01:CDS | 35.0% | |
| ! | CTCTTGGTGAAATGAGTTTT+AGG | + | Chr8:29568869-29568888 | MsG0880043715.01.T01:three_prime_UTR | 35.0% |
| !! | TGTTACATCGTTTTGAATGG+TGG | + | Chr8:29568832-29568851 | MsG0880043715.01.T01:three_prime_UTR | 35.0% |
| !! | TTTAAGAAGGTTTCTGGTGT+TGG | + | Chr8:29568402-29568421 | MsG0880043715.01.T01:CDS | 35.0% |
| !!! | TGAAATGAGTTTTAGGTTGG+CGG | + | Chr8:29568876-29568895 | MsG0880043715.01.T01:three_prime_UTR | 35.0% |
| !!! | TGGTGAAATGAGTTTTAGGT+TGG | + | Chr8:29568873-29568892 | MsG0880043715.01.T01:three_prime_UTR | 35.0% |
| !!! | TTTTGAGTTGATGAGAGTGA+TGG | + | Chr8:29568623-29568642 | MsG0880043715.01.T01:CDS | 35.0% |
| AAGGTTATGAAGCACGGATA+CGG | + | Chr8:29568012-29568031 | MsG0880043715.01.T01:intron | 40.0% | |
| AATAACCTGTGCAATAACCG+CGG | - | Chr8:29567813-29567832 | None:intergenic | 40.0% | |
| AATCATCCACCGAAGAAAGA+AGG | - | Chr8:29567565-29567584 | None:intergenic | 40.0% | |
| ACAATCAACGAAAACAACGC+CGG | - | Chr8:29567755-29567774 | None:intergenic | 40.0% | |
| CTTAATCTTGTGAGATGTGG+TGG | + | Chr8:29568498-29568517 | MsG0880043715.01.T01:CDS | 40.0% | |
| GATGATTATCGGACTGTTGT+TGG | + | Chr8:29567578-29567597 | MsG0880043715.01.T01:CDS | 40.0% | |
| GATTCACAGCCTTCTTTCTT+CGG | + | Chr8:29567553-29567572 | MsG0880043715.01.T01:CDS | 40.0% | |
| TTGAGTAGTGATTCGTCGTT+TGG | + | Chr8:29568732-29568751 | MsG0880043715.01.T01:CDS | 40.0% | |
| !! | AAGTGTTGAGGCGTTTAAGA+AGG | + | Chr8:29568389-29568408 | MsG0880043715.01.T01:CDS | 40.0% |
| !! | ACATCTCTGATTTAGGTTGC+TGG | + | Chr8:29568357-29568376 | MsG0880043715.01.T01:intron | 40.0% |
| !! | TAGTGGTACGTCGTTTTGAA+TGG | + | Chr8:29568773-29568792 | MsG0880043715.01.T01:exon | 40.0% |
| !! | TTTCTACTGGTTCGAGTATG+AGG | + | Chr8:29568448-29568467 | MsG0880043715.01.T01:CDS | 40.0% |
| AACGAATCTTTCACCGTCCT+TGG | - | Chr8:29567665-29567684 | None:intergenic | 45.0% | |
| AAGAAGGCTGTGAATCGTAG+CGG | - | Chr8:29567549-29567568 | None:intergenic | 45.0% | |
| AAGTGTTAAGGGGCAGAGTA+AGG | + | Chr8:29568599-29568618 | MsG0880043715.01.T01:CDS | 45.0% | |
| ACCATCGTAGCACGATTCAA+CGG | - | Chr8:29567716-29567735 | None:intergenic | 45.0% | |
| GATCTGCTCGATCTTTCTAC+TGG | + | Chr8:29568435-29568454 | MsG0880043715.01.T01:CDS | 45.0% | |
| GATTGTTGAAGATGCTTCCG+CGG | + | Chr8:29567793-29567812 | MsG0880043715.01.T01:CDS | 45.0% | |
| GTTCGTAGTCACGGTAATGT+TGG | + | Chr8:29568708-29568727 | MsG0880043715.01.T01:CDS | 45.0% | |
| TATGAGGACGAGGGAAAATG+CGG | + | Chr8:29568464-29568483 | MsG0880043715.01.T01:CDS | 45.0% | |
| ! | GAGGCGTTTAAGAAGGTTTC+TGG | + | Chr8:29568396-29568415 | MsG0880043715.01.T01:CDS | 45.0% |
| ! | GATGGTGATGTTCGTAGTCA+CGG | + | Chr8:29568699-29568718 | MsG0880043715.01.T01:CDS | 45.0% |
| ! | TGAATGGTGGTTCGTCGTTT+TGG | + | Chr8:29568845-29568864 | MsG0880043715.01.T01:three_prime_UTR | 45.0% |
| !! | GAGTTGATGAGAGTGATGGT+TGG | + | Chr8:29568627-29568646 | MsG0880043715.01.T01:CDS | 45.0% |
| !! | GGTTCAGTTTGGTGTTGTTC+CGG | + | Chr8:29567733-29567752 | MsG0880043715.01.T01:CDS | 45.0% |
| !! | GTTGGTGATGTTCGTAGTCA+CGG | + | Chr8:29568645-29568664 | MsG0880043715.01.T01:CDS | 45.0% |
| !! | TGGTACGTCGTTTTGAATGG+TGG | + | Chr8:29568776-29568795 | MsG0880043715.01.T01:exon | 45.0% |
| ATGAAGCACGGATACGGACA+TGG | + | Chr8:29568018-29568037 | MsG0880043715.01.T01:intron | 50.0% | |
| CACGGTGATGTTCGTAGTCA+CGG | + | Chr8:29568663-29568682 | MsG0880043715.01.T01:CDS | 50.0% | |
| CACGGTGATGTTCGTAGTGA+TGG | + | Chr8:29568681-29568700 | MsG0880043715.01.T01:CDS | 50.0% | |
| CGTGCTACGATGGTTCAGTT+TGG | + | Chr8:29567722-29567741 | MsG0880043715.01.T01:CDS | 50.0% | |
| GAATCTTTCACCGTCCTTGG+CGG | - | Chr8:29567662-29567681 | None:intergenic | 50.0% | |
| TCACAGCCTTCTTTCTTCGG+TGG | + | Chr8:29567556-29567575 | MsG0880043715.01.T01:CDS | 50.0% | |
| TCCGTTGAATCGTGCTACGA+TGG | + | Chr8:29567712-29567731 | MsG0880043715.01.T01:CDS | 50.0% | |
| TGGTTCGAGTATGAGGACGA+GGG | + | Chr8:29568455-29568474 | MsG0880043715.01.T01:CDS | 50.0% | |
| ! | TGTGAGATGTGGTGGAGATG+TGG | + | Chr8:29568506-29568525 | MsG0880043715.01.T01:CDS | 50.0% |
| !! | GATTTAGGTTGCTGGTTGCG+AGG | + | Chr8:29568365-29568384 | MsG0880043715.01.T01:intron | 50.0% |
| !! | GGTTCGTCGTTTTGGTCTCT+TGG | + | Chr8:29568853-29568872 | MsG0880043715.01.T01:three_prime_UTR | 50.0% |
| !! | GGTTTCTGGTGTTGGAGTGT+TGG | + | Chr8:29568410-29568429 | MsG0880043715.01.T01:CDS | 50.0% |
| !! | TGGTTGCGAGGAAAGTGTTG+AGG | + | Chr8:29568377-29568396 | MsG0880043715.01.T01:CDS | 50.0% |
| !! | TTCTGGTGTTGGAGTGTTGG+CGG | + | Chr8:29568413-29568432 | MsG0880043715.01.T01:CDS | 50.0% |
| AAGGCTGTGAATCGTAGCGG+CGG | - | Chr8:29567546-29567565 | None:intergenic | 55.0% | |
| ACGGATACGGACATGGACAC+CGG | + | Chr8:29568025-29568044 | MsG0880043715.01.T01:intron | 55.0% | |
| GACGAGGGAAAATGCGGTGT+CGG | + | Chr8:29568470-29568489 | MsG0880043715.01.T01:CDS | 55.0% | |
| GGCTGGAGATGTAAGAGACG+CGG | + | Chr8:29568530-29568549 | MsG0880043715.01.T01:CDS | 55.0% | |
| GTCAGTGTCCATGTCATGCC+CGG | - | Chr8:29568047-29568066 | None:intergenic | 55.0% | |
| TGCTTCCGCGGTTATTGCAC+AGG | + | Chr8:29567805-29567824 | MsG0880043715.01.T01:CDS | 55.0% | |
| TGGAGATGTAAGAGACGCGG+TGG | + | Chr8:29568533-29568552 | MsG0880043715.01.T01:CDS | 55.0% | |
| ! | CTGGTTCGAGTATGAGGACG+AGG | + | Chr8:29568454-29568473 | MsG0880043715.01.T01:CDS | 55.0% |
| ! | GAGATGTGGTGGAGATGTGG+TGG | + | Chr8:29568509-29568528 | MsG0880043715.01.T01:CDS | 55.0% |
| CATGGACACCGGGCATGACA+TGG | + | Chr8:29568036-29568055 | MsG0880043715.01.T01:intron | 60.0% | |
| CCGTCCTTGGCGGTGAAGAA+CGG | - | Chr8:29567652-29567671 | None:intergenic | 60.0% | |
| CCGTTCTTCACCGCCAAGGA+CGG | + | Chr8:29567649-29567668 | MsG0880043715.01.T01:CDS | 60.0% | |
| CGGATACGGACATGGACACC+GGG | + | Chr8:29568026-29568045 | MsG0880043715.01.T01:intron | 60.0% | |
| GTAAGAGACGCGGTGGCGTT+TGG | + | Chr8:29568540-29568559 | MsG0880043715.01.T01:CDS | 60.0% | |
| GTCACCGTTCTTCACCGCCA+AGG | + | Chr8:29567645-29567664 | MsG0880043715.01.T01:CDS | 60.0% | |
| ! | GTGTCGTGTTGCGTCGGTGT+CGG | + | Chr8:29568126-29568145 | MsG0880043715.01.T01:intron | 60.0% |
| ! | TGTGGTGGAGATGTGGTGGC+TGG | + | Chr8:29568513-29568532 | MsG0880043715.01.T01:CDS | 60.0% |
| !! | GTGGCGTTTGGTGCGATGGA+TGG | + | Chr8:29568552-29568571 | MsG0880043715.01.T01:CDS | 60.0% |
| !! | GTGTCGGTGTCGTGTTGCGT+CGG | + | Chr8:29568120-29568139 | MsG0880043715.01.T01:intron | 60.0% |
| GCTGTGAATCGTAGCGGCGG+CGG | - | Chr8:29567543-29567562 | None:intergenic | 65.0% | |
| GGATTGAACAGCAGCGGCGG+CGG | - | Chr8:29567522-29567541 | None:intergenic | 65.0% | |
| !! | GGCGGATTGAACAGCAGCGG+CGG | - | Chr8:29567525-29567544 | None:intergenic | 65.0% |
| !! | GGCGGCGGATTGAACAGCAG+CGG | - | Chr8:29567528-29567547 | None:intergenic | 65.0% |
| TTGAACAGCAGCGGCGGCGG+AGG | - | Chr8:29567519-29567538 | None:intergenic | 70.0% | |
| !! | CGCGGTGGCGTTTGGTGCGA+TGG | + | Chr8:29568548-29568567 | MsG0880043715.01.T01:CDS | 70.0% |
| CGCGTCGAGAACACCGCGCG+TGG | - | Chr8:29567465-29567484 | None:intergenic | 75.0% | |
| GCGCGGTGTTCTCGACGCGC+TGG | + | Chr8:29567466-29567485 | MsG0880043715.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 29567458 | 29569047 | 29567458 | ID=MsG0880043715.01;Name=MsG0880043715.01 |
| Chr8 | mRNA | 29567458 | 29569047 | 29567458 | ID=MsG0880043715.01.T01;Parent=MsG0880043715.01;Name=MsG0880043715.01.T01;_AED=0.29;_eAED=0.29;_QI=0|1|0.5|1|1|1|2|256|262 |
| Chr8 | exon | 29567458 | 29567826 | 29567458 | ID=MsG0880043715.01.T01:exon:6981;Parent=MsG0880043715.01.T01 |
| Chr8 | exon | 29568372 | 29569047 | 29568372 | ID=MsG0880043715.01.T01:exon:6982;Parent=MsG0880043715.01.T01 |
| Chr8 | CDS | 29567458 | 29567826 | 29567458 | ID=MsG0880043715.01.T01:cds;Parent=MsG0880043715.01.T01 |
| Chr8 | CDS | 29568372 | 29568791 | 29568372 | ID=MsG0880043715.01.T01:cds;Parent=MsG0880043715.01.T01 |
| Chr8 | three_prime_UTR | 29568792 | 29569047 | 29568792 | ID=MsG0880043715.01.T01:three_prime_utr;Parent=MsG0880043715.01.T01 |
| Gene Sequence |
| Protein sequence |
