AlfalfaGEDB | MsG0880047491.01

M. sativa cultivar ZhongmuNo.1 / MsG0880047491.01

KEGG: -
GO: -
PFAM: PF05553

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047491.01.T01	XP_003610146.1	95.161	124	6	0	1	124	1	124	2.16E-80	242

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047491.01.T01	G7JD05	95.161	124	6	0	1	124	1	124	1.03e-80	242

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048345.01	MsG0880047491.01	0.835822	1.341705e-56	1.659639e-53
MsG0080048348.01	MsG0880047491.01	0.824739	6.856204e-54	6.126429e-51
MsG0180000019.01	MsG0880047491.01	0.846097	2.690817e-59	4.580489e-56
MsG0180000337.01	MsG0880047491.01	0.829784	4.241698e-55	4.385932e-52
MsG0180000930.01	MsG0880047491.01	0.830595	2.688007e-55	2.845077e-52
MsG0180001050.01	MsG0880047491.01	0.830538	2.776091e-55	2.933221e-52
MsG0180001053.01	MsG0880047491.01	0.855889	4.660275e-62	1.092093e-58
MsG0880044669.01	MsG0880047491.01	0.807254	5.530184e-50	3.065500e-47
MsG0880045272.01	MsG0880047491.01	0.812337	4.461165e-51	2.828901e-48
MsG0880045275.01	MsG0880047491.01	0.827431	1.570407e-54	1.515824e-51
MsG0880045277.01	MsG0880047491.01	0.804483	2.113959e-49	1.091192e-46
MsG0880045441.01	MsG0880047491.01	0.836071	1.159693e-56	1.445452e-53
MsG0880045677.01	MsG0880047491.01	0.808202	3.477147e-50	1.975949e-47
MsG0880045861.01	MsG0880047491.01	0.814188	1.749873e-51	1.166208e-48
MsG0880046863.01	MsG0880047491.01	0.804139	2.493005e-49	1.275571e-46
MsG0880046996.01	MsG0880047491.01	0.837537	4.902958e-57	6.388324e-54
MsG0880047081.01	MsG0880047491.01	0.839371	1.648923e-57	2.272576e-54
MsG0880047256.01	MsG0880047491.01	0.810985	8.779754e-51	5.370822e-48
MsG0880047486.01	MsG0880047491.01	0.915391	6.223160e-85	1.622005e-80
MsG0880047491.01	MsG0880047531.01	0.805528	1.278186e-49	6.777753e-47
MsG0180003830.01	MsG0880047491.01	0.805173	1.516616e-49	7.969494e-47
MsG0180004393.01	MsG0880047491.01	0.807134	5.862199e-50	3.239363e-47
MsG0180004562.01	MsG0880047491.01	0.886437	3.422695e-72	2.444958e-68
MsG0180004752.01	MsG0880047491.01	0.844594	6.863617e-59	1.113550e-55
MsG0180005328.01	MsG0880047491.01	0.801423	9.068513e-49	4.329119e-46
MsG0180005356.01	MsG0880047491.01	0.815147	1.072908e-51	7.338035e-49
MsG0180005357.01	MsG0880047491.01	0.825691	4.083557e-54	3.749079e-51
MsG0180005358.01	MsG0880047491.01	0.814134	1.798397e-51	1.196797e-48
MsG0780040480.01	MsG0880047491.01	0.831565	1.553458e-55	1.692216e-52
MsG0780041133.01	MsG0880047491.01	0.801233	9.917139e-49	4.711776e-46
MsG0780041299.01	MsG0880047491.01	0.800649	1.305517e-48	6.111517e-46
MsG0780041316.01	MsG0880047491.01	0.815150	1.071179e-51	7.326858e-49
MsG0780041556.01	MsG0880047491.01	0.849300	3.533418e-60	6.669114e-57
MsG0880042810.01	MsG0880047491.01	0.817567	3.083723e-52	2.253658e-49
MsG0880043109.01	MsG0880047491.01	0.800104	1.685701e-48	7.782707e-46
MsG0880043185.01	MsG0880047491.01	0.831993	1.218339e-55	1.344092e-52
MsG0880043685.01	MsG0880047491.01	0.802231	6.187319e-49	3.014932e-46
MsG0880043715.01	MsG0880047491.01	0.831967	1.236236e-55	1.362770e-52
MsG0280011041.01	MsG0880047491.01	0.807191	5.701580e-50	3.155385e-47
MsG0280011042.01	MsG0880047491.01	0.805923	1.055816e-49	5.655942e-47
MsG0280011053.01	MsG0880047491.01	0.815656	8.266684e-52	5.733880e-49
MsG0280011152.01	MsG0880047491.01	0.801926	7.150133e-49	3.457069e-46
MsG0280011165.01	MsG0880047491.01	0.809722	1.644114e-50	9.726612e-48
MsG0280011288.01	MsG0880047491.01	0.806311	8.747785e-50	4.732701e-47
MsG0280011350.01	MsG0880047491.01	0.821555	3.794049e-53	3.096825e-50
MsG0280011473.01	MsG0880047491.01	0.805330	1.406580e-49	7.420852e-47
MsG0380011613.01	MsG0880047491.01	0.857191	1.930443e-62	4.725152e-59
MsG0380013272.01	MsG0880047491.01	0.808934	2.426803e-50	1.405690e-47
MsG0380013278.01	MsG0880047491.01	0.808129	3.604399e-50	2.044237e-47
MsG0380013300.01	MsG0880047491.01	0.805081	1.585316e-49	8.310764e-47
MsG0680030961.01	MsG0880047491.01	0.823191	1.581599e-53	1.352369e-50
MsG0680031429.01	MsG0880047491.01	0.803269	3.777933e-49	1.890280e-46
MsG0680034663.01	MsG0880047491.01	0.808649	2.792305e-50	1.605317e-47
MsG0680035496.01	MsG0880047491.01	0.807023	6.188517e-50	3.409897e-47
MsG0480018500.01	MsG0880047491.01	0.812102	5.018787e-51	3.162209e-48
MsG0480018807.01	MsG0880047491.01	0.809133	2.199846e-50	1.281153e-47
MsG0480019295.01	MsG0880047491.01	0.810029	1.411923e-50	8.420667e-48
MsG0480019726.01	MsG0880047491.01	0.838327	3.071990e-57	4.100318e-54
MsG0480021131.01	MsG0880047491.01	0.807624	4.615449e-50	2.583144e-47
MsG0480021135.01	MsG0880047491.01	0.828982	6.641706e-55	6.706136e-52
MsG0480022125.01	MsG0880047491.01	0.850804	1.340013e-60	2.656640e-57
MsG0480022736.01	MsG0880047491.01	0.825167	5.433823e-54	4.915062e-51
MsG0480022797.01	MsG0880047491.01	0.829139	6.083747e-55	6.171997e-52
MsG0580024154.01	MsG0880047491.01	0.835059	2.091444e-56	2.528437e-53
MsG0580024871.01	MsG0880047491.01	0.824651	7.193008e-54	6.411094e-51
MsG0580025876.01	MsG0880047491.01	0.816398	5.643720e-52	3.994727e-49
MsG0380015010.01	MsG0880047491.01	0.809590	1.755063e-50	1.034683e-47
MsG0380016494.01	MsG0880047491.01	0.801351	9.380056e-49	4.469858e-46
MsG0380016839.01	MsG0880047491.01	0.817740	2.818826e-52	2.069985e-49
MsG0380017690.01	MsG0880047491.01	0.812932	3.304771e-51	2.128679e-48
MsG0380017766.01	MsG0880047491.01	0.834006	3.845277e-56	4.504635e-53
MsG0580027227.01	MsG0880047491.01	0.800898	1.161303e-48	5.471052e-46
MsG0580027641.01	MsG0880047491.01	0.802344	5.865997e-49	2.866663e-46
MsG0580027733.01	MsG0880047491.01	0.805967	1.033541e-49	5.542985e-47
MsG0580028758.01	MsG0880047491.01	0.841624	4.245881e-58	6.275145e-55
MsG0580028999.01	MsG0880047491.01	0.843688	1.201661e-58	1.895003e-55
MsG0580029583.01	MsG0880047491.01	0.835142	1.992755e-56	2.415201e-53
MsG0580029930.01	MsG0880047491.01	0.852719	3.837670e-61	8.092565e-58
MsG0680030545.01	MsG0880047491.01	0.830889	2.276920e-55	2.431077e-52
MsG0280007369.01	MsG0880047491.01	0.801294	9.636662e-49	4.585513e-46
MsG0280007759.01	MsG0880047491.01	0.841961	3.458801e-58	5.165888e-55
MsG0280007760.01	MsG0880047491.01	0.836583	8.594902e-57	1.087792e-53
MsG0280007840.01	MsG0880047491.01	0.801656	8.125103e-49	3.901502e-46
MsG0280008405.01	MsG0880047491.01	0.809308	2.017756e-50	1.180568e-47
MsG0280009394.01	MsG0880047491.01	0.847701	9.788914e-60	1.754724e-56
MsG0680035741.01	MsG0880047491.01	0.809576	1.767041e-50	1.041349e-47
MsG0780037599.01	MsG0880047491.01	0.806437	8.230442e-50	4.467133e-47
MsG0780038123.01	MsG0880047491.01	0.844906	5.655009e-59	9.264804e-56
MsG0780038924.01	MsG0880047491.01	0.817858	2.651454e-52	1.953307e-49
MsG0780039190.01	MsG0880047491.01	0.809695	1.665974e-50	9.848737e-48
MsG0780039334.01	MsG0880047491.01	0.805971	1.031807e-49	5.534191e-47
MsG0780039472.01	MsG0880047491.01	0.814999	1.157018e-51	7.882103e-49
MsG0780039877.01	MsG0880047491.01	0.820632	6.189844e-53	4.923672e-50
MsG0780039968.01	MsG0880047491.01	0.814249	1.696430e-51	1.132462e-48
MsG0780040144.01	MsG0880047491.01	0.826876	2.132777e-54	2.025388e-51

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047491.01.T01	MTR_4g128470	95.161	124	6	0	1	124	1	124	2.62e-84	242

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047491.01.T01	AT5G57510	48.760	121	56	5	6	123	3	120	5.26e-26	95.1

Find 18 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGCAGGAACAAAAGAGTTC+TGG	0.308826	8:-87065041	MsG0880047491.01.T01:CDS
CATTGCAGCTACGTTATTGC+AGG	0.374536	8:-87065057	MsG0880047491.01.T01:CDS
CTCCATCTCCTTGTCTTCAA+AGG	0.416512	8:-87065177	MsG0880047491.01.T01:CDS
GTTCCATGTGTGGTTGAGTA+TGG	0.434971	8:+87065312	None:intergenic
CCTACGAACGGCGGTGCAAA+AGG	0.478843	8:-87065356	MsG0880047491.01.T01:CDS
CTTATTGTCCTTTGAAGACA+AGG	0.489660	8:+87065169	None:intergenic
CTAAGTTTCAACGATCGTCC+TGG	0.511331	8:-87065247	MsG0880047491.01.T01:CDS
GTCCTTTGAAGACAAGGAGA+TGG	0.521798	8:+87065175	None:intergenic
TTCAGATGAAACTGATTCAG+AGG	0.544944	8:-87065209	MsG0880047491.01.T01:CDS
GAAGAATACATCAACAATCC+AGG	0.547215	8:+87065229	None:intergenic
CCACCATACTCAACCACACA+TGG	0.550819	8:-87065315	MsG0880047491.01.T01:CDS
TCAGATGAAACTGATTCAGA+GGG	0.570568	8:-87065208	MsG0880047491.01.T01:CDS
CCATGTGTGGTTGAGTATGG+TGG	0.585997	8:+87065315	None:intergenic
TTTGCACCGCCGTTCGTAGG+CGG	0.592132	8:+87065359	None:intergenic
GGATGACATTGATAAGAGAT+CGG	0.600101	8:-87065131	MsG0880047491.01.T01:CDS
TCAATGTCATCCTCATCTGA+AGG	0.601031	8:+87065142	None:intergenic
TCTACTAAGCCGCCTACGAA+CGG	0.672450	8:-87065368	MsG0880047491.01.T01:CDS
ACTAAGCCGCCTACGAACGG+CGG	0.714763	8:-87065365	MsG0880047491.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATAAGTTTTCCTTCAGATG+AGG	-	Chr8:87065284-87065303	MsG0880047491.01.T01:CDS	30.0%
	GAAGAATACATCAACAATCC+AGG	+	Chr8:87065210-87065229	None:intergenic	35.0%
	GGATGACATTGATAAGAGAT+CGG	-	Chr8:87065305-87065324	MsG0880047491.01.T01:CDS	35.0%
!	CTTATTGTCCTTTGAAGACA+AGG	+	Chr8:87065270-87065289	None:intergenic	35.0%
!	TCAGATGAAACTGATTCAGA+GGG	-	Chr8:87065228-87065247	MsG0880047491.01.T01:CDS	35.0%
!	TTCAGATGAAACTGATTCAG+AGG	-	Chr8:87065227-87065246	MsG0880047491.01.T01:CDS	35.0%
	TCAATGTCATCCTCATCTGA+AGG	+	Chr8:87065297-87065316	None:intergenic	40.0%
	TTGCAGGAACAAAAGAGTTC+TGG	-	Chr8:87065395-87065414	MsG0880047491.01.T01:CDS	40.0%
	AACGCTGCAAAAATGCTTCG+CGG	-	Chr8:87065144-87065163	MsG0880047491.01.T01:CDS	45.0%
	CATTGCAGCTACGTTATTGC+AGG	-	Chr8:87065379-87065398	MsG0880047491.01.T01:CDS	45.0%
	CTAAGTTTCAACGATCGTCC+TGG	-	Chr8:87065189-87065208	MsG0880047491.01.T01:CDS	45.0%
	CTCCATCTCCTTGTCTTCAA+AGG	-	Chr8:87065259-87065278	MsG0880047491.01.T01:CDS	45.0%
	GTTCCATGTGTGGTTGAGTA+TGG	+	Chr8:87065127-87065146	None:intergenic	45.0%
!	GTCCTTTGAAGACAAGGAGA+TGG	+	Chr8:87065264-87065283	None:intergenic	45.0%
!	TTTTTGCAGCGTTCCATGTG+TGG	+	Chr8:87065137-87065156	None:intergenic	45.0%
	CCACCATACTCAACCACACA+TGG	-	Chr8:87065121-87065140	MsG0880047491.01.T01:CDS	50.0%
	CCATGTGTGGTTGAGTATGG+TGG	+	Chr8:87065124-87065143	None:intergenic	50.0%
	TCTACTAAGCCGCCTACGAA+CGG	-	Chr8:87065068-87065087	MsG0880047491.01.T01:CDS	50.0%
	ACTAAGCCGCCTACGAACGG+CGG	-	Chr8:87065071-87065090	MsG0880047491.01.T01:CDS	60.0%
	CCTACGAACGGCGGTGCAAA+AGG	-	Chr8:87065080-87065099	MsG0880047491.01.T01:CDS	60.0%
	TTTGCACCGCCGTTCGTAGG+CGG	+	Chr8:87065080-87065099	None:intergenic	60.0%
!	CCTTTTGCACCGCCGTTCGT+AGG	+	Chr8:87065083-87065102	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	87065036	87065422	87065036	ID=MsG0880047491.01;Name=MsG0880047491.01
Chr8	mRNA	87065036	87065422	87065036	ID=MsG0880047491.01.T01;Parent=MsG0880047491.01;Name=MsG0880047491.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|128
Chr8	exon	87065036	87065422	87065036	ID=MsG0880047491.01.T01:exon:15701;Parent=MsG0880047491.01.T01
Chr8	CDS	87065036	87065422	87065036	ID=MsG0880047491.01.T01:cds;Parent=MsG0880047491.01.T01

Gene Sequence

>MsG0880047491.01.T01

ATGAGCACACACAATCAAAAGCAGAACAAGTCTCTACTAAGCCGCCTACGAACGGCGGTGCAAAAGGTGAAGCTTCTTATAAGCTCCACCATACTCAACCACACATGGAACGCTGCAAAAATGCTTCGCGGTGCTTCTTTGAGCAATCGACAACTAAGTTTCAACGATCGTCCTGGATTGTTGATGTATTCTTCAGATGAAACTGATTCAGAGGGTTCTGTTTCTCCATCTCCTTGTCTTCAAAGGACAATAAGTTTTCCTTCAGATGAGGATGACATTGATAAGAGATCGGAGATTTTCATAGCTAATTTCAGACGACAACTTTTGTTAGAGAGACAAATCTCATTGCAGCTACGTTATTGCAGGAACAAAAGAGTTCTGGATTGA

Protein sequence

>MsG0880047491.01.T01

MSTHNQKQNKSLLSRLRTAVQKVKLLISSTILNHTWNAAKMLRGASLSNRQLSFNDRPGLLMYSSDETDSEGSVSPSPCLQRTISFPSDEDDIDKRSEIFIANFRRQLLLERQISLQLRYCRNKRVLD*