AlfalfaGEDB | MsG0480021135.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021135.01

KEGG: -
GO: GO:0004553 GO:0005975
PFAM: PF00232

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021135.01.T01	XP_013456264.1	96.941	523	12	2	1	523	1	519	0	1045

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021135.01.T01	Q5Z9Z0	62.526	475	178	0	37	511	28	502	0	659

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021135.01.T01	A0A072UWR5	96.941	523	12	2	1	523	1	519	0.0	1045

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048345.01	MsG0480021135.01	0.843765	1.146016e-58	1.811440e-55
MsG0080048348.01	MsG0480021135.01	0.808257	3.384667e-50	1.926200e-47
MsG0080048349.01	MsG0480021135.01	0.801205	1.005064e-48	4.771621e-46
MsG0080048352.01	MsG0480021135.01	0.801390	9.208043e-49	4.392110e-46
MsG0180000019.01	MsG0480021135.01	0.847519	1.098965e-59	1.958535e-56
MsG0180000930.01	MsG0480021135.01	0.821297	4.351093e-53	3.525962e-50
MsG0180001050.01	MsG0480021135.01	0.899362	2.136826e-77	2.633706e-73
MsG0180001053.01	MsG0480021135.01	0.866613	2.507835e-65	8.505170e-62
MsG0180003240.01	MsG0480021135.01	0.818319	2.086185e-52	1.556446e-49
MsG0180003830.01	MsG0480021135.01	0.804935	1.701077e-49	8.884591e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MsG0480021135.01.T01	MTR_4g066590	96.941	523	12	2	1	523	1	519	1045
MsG0480021135.01.T01	MTR_4g066630	88.337	523	58	2	1	523	5	524	940
MsG0480021135.01.T01	MTR_4g066580	74.662	517	129	1	4	520	8	522	836
MsG0480021135.01.T01	MTR_4g066630	89.278	457	47	1	1	457	5	459	831
MsG0480021135.01.T01	MTR_3g028720	73.025	519	136	2	6	520	3	521	818
MsG0480021135.01.T01	MTR_4g067040	95.610	410	15	1	1	410	1	407	813
MsG0480021135.01.T01	MTR_4g067040	95.116	389	16	1	1	389	1	386	769
MsG0480021135.01.T01	MTR_4g066630	89.573	422	42	1	1	422	5	424	767
MsG0480021135.01.T01	MTR_2g098540	71.343	499	141	1	13	511	9	505	758
MsG0480021135.01.T01	MTR_2g098790	68.493	511	158	2	1	511	5	512	752

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MsG0480021135.01.T01	AT5G44640	53.965	517	226	5	1	516	1	506	577
MsG0480021135.01.T01	AT2G44450	55.858	478	206	2	37	514	31	503	570
MsG0480021135.01.T01	AT5G42260	54.724	508	218	5	1	507	1	497	569
MsG0480021135.01.T01	AT2G44480	54.792	480	216	1	36	515	34	512	565
MsG0480021135.01.T01	AT2G44480	54.792	480	216	1	36	515	65	543	564
MsG0480021135.01.T01	AT3G60130	55.672	476	205	3	37	511	30	500	560
MsG0480021135.01.T01	AT2G25630	51.786	504	220	4	14	516	7	488	538
MsG0480021135.01.T01	AT5G24550	52.965	489	225	5	37	521	32	519	535
MsG0480021135.01.T01	AT3G60140	50.100	499	238	6	16	511	12	502	526
MsG0480021135.01.T01	AT5G24540	50.577	520	250	6	8	521	1	519	525

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Find 101 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGACTTTCAAGACTATG+CGG	0.704652	4:+54177424	MsG0480021135.01.T01:CDS
AAAAGATATCAAAAGCTCTC+AGG	0.470702	4:+54178919	MsG0480021135.01.T01:CDS
AAAATCAGTGATGGAAACAA+TGG	0.553564	4:+54177040	MsG0480021135.01.T01:CDS
AAATCACTCACATGATGAGA+GGG	0.589603	4:-54177327	None:intergenic
AACACTTACATTTAGACCAA+TGG	0.493905	4:-54178238	None:intergenic
AACCAAGAAGAACATCGACC+TGG	0.677559	4:-54177548	None:intergenic
AACGAGAAAGTGAATCTGTT+AGG	0.479548	4:-54178089	None:intergenic
AACGCTGCACCACTGCAAAT+AGG	0.417516	4:+54176881	MsG0480021135.01.T01:CDS
AAGGGTTATACTGTGAGATT+TGG	0.344341	4:+54178865	MsG0480021135.01.T01:CDS
AAGTATCAAGTGTCTCAAAA+GGG	0.587635	4:+54177671	MsG0480021135.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGACTTTCAAGACTATG+CGG	+	Chr4:54177424-54177443	MsG0480021135.01.T01:CDS	30.0%
AAAAGATATCAAAAGCTCTC+AGG	+	Chr4:54178919-54178938	MsG0480021135.01.T01:CDS	30.0%
AACACTTACATTTAGACCAA+TGG	-	Chr4:54178241-54178260	None:intergenic	30.0%
AAGTATCAAGTGTCTCAAAA+GGG	+	Chr4:54177671-54177690	MsG0480021135.01.T01:CDS	30.0%
AGATATAAAGTAGGCCAAAT+AGG	-	Chr4:54178128-54178147	None:intergenic	30.0%
AGCAAATCACTTTCATATCA+TGG	+	Chr4:54178451-54178470	MsG0480021135.01.T01:intron	30.0%
AGTTTACAAAAGAGCAATCT+AGG	+	Chr4:54177971-54177990	MsG0480021135.01.T01:CDS	30.0%
ATGTGCAACTAAATATGGTT+CGG	-	Chr4:54177606-54177625	None:intergenic	30.0%
CTTCAAAACATTTGGAGATA+GGG	+	Chr4:54177454-54177473	MsG0480021135.01.T01:CDS	30.0%
GAACTTTGCTTCAAAACATT+TGG	+	Chr4:54177446-54177465	MsG0480021135.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	54176684	54179095	54176684	ID=MsG0480021135.01;Name=MsG0480021135.01
Chr4	mRNA	54176684	54179095	54176684	ID=MsG0480021135.01.T01;Parent=MsG0480021135.01;Name=MsG0480021135.01.T01;_AED=0.27;_eAED=0.27;_QI=116\|1\|1\|1\|1\|1\|7\|93\|523
Chr4	exon	54176684	54177338	54176684	ID=MsG0480021135.01.T01:exon:28739;Parent=MsG0480021135.01.T01
Chr4	exon	54177424	54177795	54177424	ID=MsG0480021135.01.T01:exon:28740;Parent=MsG0480021135.01.T01
Chr4	exon	54177900	54178117	54177900	ID=MsG0480021135.01.T01:exon:28741;Parent=MsG0480021135.01.T01
Chr4	exon	54178220	54178251	54178220	ID=MsG0480021135.01.T01:exon:28742;Parent=MsG0480021135.01.T01
Chr4	exon	54178345	54178447	54178345	ID=MsG0480021135.01.T01:exon:28743;Parent=MsG0480021135.01.T01
Chr4	exon	54178600	54178708	54178600	ID=MsG0480021135.01.T01:exon:28744;Parent=MsG0480021135.01.T01
Chr4	exon	54178804	54179095	54178804	ID=MsG0480021135.01.T01:exon:28745;Parent=MsG0480021135.01.T01
Chr4	five_prime_UTR	54176684	54176799	54176684	ID=MsG0480021135.01.T01:five_prime_utr;Parent=MsG0480021135.01.T01
Chr4	CDS	54176800	54177338	54176800	ID=MsG0480021135.01.T01:cds;Parent=MsG0480021135.01.T01
Chr4	CDS	54177424	54177795	54177424	ID=MsG0480021135.01.T01:cds;Parent=MsG0480021135.01.T01
Chr4	CDS	54177900	54178117	54177900	ID=MsG0480021135.01.T01:cds;Parent=MsG0480021135.01.T01
Chr4	CDS	54178220	54178251	54178220	ID=MsG0480021135.01.T01:cds;Parent=MsG0480021135.01.T01
Chr4	CDS	54178345	54178447	54178345	ID=MsG0480021135.01.T01:cds;Parent=MsG0480021135.01.T01
Chr4	CDS	54178600	54178708	54178600	ID=MsG0480021135.01.T01:cds;Parent=MsG0480021135.01.T01
Chr4	CDS	54178804	54179002	54178804	ID=MsG0480021135.01.T01:cds;Parent=MsG0480021135.01.T01
Chr4	three_prime_UTR	54179003	54179095	54179003	ID=MsG0480021135.01.T01:three_prime_utr;Parent=MsG0480021135.01.T01

Gene Sequence

>MsG0480021135.01.T01

ATGAGATATTTCCTATTTTCTCTCTTGTTGTCTATAGTTTCAACATTCATTATTACTTTAACAGAAGGTCAAGAAATAATTAACGCTGCACCACTGCAAATAGGTTCATTGAATAGGAATGATTTCCCAGAAGGCTTCATATTTGGCACAGCATCTTCTGCATATCAATATGAAGGTGCAGCAAGTGAAGGTGGAAGAGGACCAAGTATATGGGATACTTTCACTCATAGATATCCTCAAAAAATCAGTGATGGAAACAATGGAGACGTTGCGGTTGACTCGTATCATCGCTATAAGGAAGATGTTGGTATCATGAAGGATATGAACTTGGATGCATATAGATTTTCCATTTCTTGGTCTAGAATACTCCCAGATGGGAAACTAAGTGGAGGAATTAATCAAGAAGGAATCGACTATTACAACAACCTCATCAATGAATTGGTGGCAAATGGATTACAACCATTTGTAACTCTTTTTCATTGGGATCTTCCTCAATCTCTAGAAGATGAATACGGCGGATTCTTAAGCCCTCTCATCATAAAAGACTTTCAAGACTATGCGGAACTTTGCTTCAAAACATTTGGAGATAGGGTAAAACATTGGATAACTTTGAATGAGCCATGGACTTACAGTCAAGATGGGTATGCAAATGGTGAAATGGCACCAGGTCGATGTTCTTCTTGGTTGAATCCAAATTGCACCGGTGGTGATTCTGGAACCGAACCATATTTAGTTGCACATTATCAACTTCTAGCTCATGCAGCAGTTGTTAATTTGTACAAGACCAAGTATCAAGTGTCTCAAAAGGGTGTGATAGGTATAACATTGGTTATTAACTATTTTGTGCCACTCTCAGATAACAAACTTGATATAAAGGCTGCTGAACGTGCTACAGACTTTATGTTTGGATGGTTTATGGATCCCTTGGCCAATGGAGATTATCCAAAAACAATGAGAGCCTTAGTAAGAACAAGGTTACCAAAGTTTACAAAAGAGCAATCTAGGCTAGTGAGTGGTTCATTTGATTTTATTGGCATAAATTACTACTCTTCTTGCTATGCTTCTGATGCACCTCAATTAAGCAATGGCAAACCTAGTTACCTAACAGATTCACTTTCTCGTTTTTCATTCGAACGTGATGGAAAAACCATTGGTCTAAATGTTGCTTCAAATTGGTTGTATGTTTATCCAAGAGCAATTCGAGACTTTCTGATACAAGTTAAAGAAAAATACAACAACCCTTTAATTTATATTACTGAAAATGGTATTAATGAGTACGATGATCCATCATTATCACTTGAGGAATCACTTATGGATACTTATAGAGTTGATTATCATTATCGTCATTTATTCTATCTTAATGAGGCAATCAAGGCTGGTGTAAATGTAAAGGGATACTTTGCATGGTCTTTGTTGGACAATTTTGAATGGCACAAGGGTTATACTGTGAGATTTGGAATGACCTTTGTGGATTACAAAAATGGTTTGAAAAGATATCAAAAGCTCTCAGGATTGTGGTTCAAGAATTTTCTCACATCAGAGAACAAAAAAGTTTACATAGATTCCATTTAA

Protein sequence

>MsG0480021135.01.T01

MRYFLFSLLLSIVSTFIITLTEGQEIINAAPLQIGSLNRNDFPEGFIFGTASSAYQYEGAASEGGRGPSIWDTFTHRYPQKISDGNNGDVAVDSYHRYKEDVGIMKDMNLDAYRFSISWSRILPDGKLSGGINQEGIDYYNNLINELVANGLQPFVTLFHWDLPQSLEDEYGGFLSPLIIKDFQDYAELCFKTFGDRVKHWITLNEPWTYSQDGYANGEMAPGRCSSWLNPNCTGGDSGTEPYLVAHYQLLAHAAVVNLYKTKYQVSQKGVIGITLVINYFVPLSDNKLDIKAAERATDFMFGWFMDPLANGDYPKTMRALVRTRLPKFTKEQSRLVSGSFDFIGINYYSSCYASDAPQLSNGKPSYLTDSLSRFSFERDGKTIGLNVASNWLYVYPRAIRDFLIQVKEKYNNPLIYITENGINEYDDPSLSLEESLMDTYRVDYHYRHLFYLNEAIKAGVNVKGYFAWSLLDNFEWHKGYTVRFGMTFVDYKNGLKRYQKLSGLWFKNFLTSENKKVYIDSI*