AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023471.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023471.01.T01 MTR_8g093850 80.519 231 21 5 1 210 1 228 9.43e-123 348
MsG0480023471.01.T01 MTR_8g093850 91.954 174 11 2 1 174 1 171 1.77e-109 315
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023471.01.T01 AT5G64010 45.495 222 104 4 2 210 3 220 1.61e-53 172

Find 41 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATCCTTGAGCCTCTTTA+AGG 0.148214 4:-85658034 MsG0480023471.01.T01:CDS
TTACCATCAGAGTTAGAATT+TGG 0.227557 4:+85658789 None:intergenic
TCGCTCCAGATGGATATTAT+TGG 0.253401 4:-85658891 MsG0480023471.01.T01:intron
CAGATGGATATTATTGGAAT+AGG 0.329644 4:-85658885 MsG0480023471.01.T01:CDS
ATCAAATGTTCTCTTGTAGA+TGG 0.339106 4:-85658000 MsG0480023471.01.T01:intron
GGGATTCCTTGGAATTGATT+TGG 0.343809 4:-85657631 MsG0480023471.01.T01:CDS
GTTAAGGTTTGTAATATAGT+AGG 0.359262 4:+85656751 None:intergenic
AGATGGATATTATTGGAATA+GGG 0.359699 4:-85658884 MsG0480023471.01.T01:CDS
TGTGCAGTTGACAAACATTA+TGG 0.378858 4:-85657755 MsG0480023471.01.T01:CDS
AATCTTTGCTGAACTCAAAC+TGG 0.398221 4:-85659165 MsG0480023471.01.T01:CDS
GTAAGAGGAATGGTGATTAG+AGG 0.400662 4:+85658096 None:intergenic
AGCACTGGAGTCCACTAGTT+TGG 0.419150 4:+85658074 None:intergenic
CTGATTTCCACTCTGACACT+TGG 0.432656 4:-85659046 MsG0480023471.01.T01:CDS
AACACTTGCCTGCGATGTGC+TGG 0.441349 4:+85656774 None:intergenic
CTTCAGATCTTCTGACTTGA+GGG 0.442503 4:+85658824 None:intergenic
AGTTTGAGTTCAGCAAAGAT+TGG 0.458340 4:+85659167 None:intergenic
ATTACAAGGTGAGAAGAATC+AGG 0.479535 4:+85659077 None:intergenic
TGACACAGCTTCGCTAGCAC+TGG 0.497449 4:+85658059 None:intergenic
GCTTCAGATCTTCTGACTTG+AGG 0.505851 4:+85658823 None:intergenic
CACTAGTTTGGTAAGAGGAA+TGG 0.543772 4:+85658086 None:intergenic
GAGTCCACTAGTTTGGTAAG+AGG 0.544770 4:+85658081 None:intergenic
GGATGAGATTGACAAGTATG+AGG 0.550352 4:+85659131 None:intergenic
GTGTGATGCCAGCACATCGC+AGG 0.551841 4:-85656782 MsG0480023471.01.T01:CDS
GAATGCCTTAAAGAGGCTCA+AGG 0.553484 4:+85658029 None:intergenic
TCTTTCAGTTTCCTCGCTAG+AGG 0.554281 4:-85659015 MsG0480023471.01.T01:CDS
GATGGATATTATTGGAATAG+GGG 0.562368 4:-85658883 MsG0480023471.01.T01:CDS
GCCATTGCTACTTAAATCGA+TGG 0.564617 4:+85659206 None:intergenic
GAGCCAAATTCTAACTCTGA+TGG 0.564766 4:-85658792 MsG0480023471.01.T01:intron
AAGAATCAGGAGCATGAACA+TGG 0.572346 4:+85659090 None:intergenic
TGCTACTTAAATCGATGGAG+TGG 0.586774 4:+85659211 None:intergenic
GGAATGCCAAATCAATTCCA+AGG 0.587376 4:+85657625 None:intergenic
TTGCTGAACTCAAACTGGAG+TGG 0.589485 4:-85659160 MsG0480023471.01.T01:CDS
TGATTTCCACTCTGACACTT+GGG 0.594053 4:-85659045 MsG0480023471.01.T01:CDS
ATGGATATTATTGGAATAGG+GGG 0.596195 4:-85658882 MsG0480023471.01.T01:CDS
AAACATTATGGCATCTGAGA+AGG 0.602177 4:-85657743 MsG0480023471.01.T01:intron
CATTCCTCTTACCAAACTAG+TGG 0.604126 4:-85658085 MsG0480023471.01.T01:CDS
TCCATCGATTTAAGTAGCAA+TGG 0.604927 4:-85659207 MsG0480023471.01.T01:exon
AATCAGTGACATGAATTACA+AGG 0.617084 4:+85659063 None:intergenic
TGAGATTGACAAGTATGAGG+TGG 0.625191 4:+85659134 None:intergenic
GAGCTTCCCAAGTGTCAGAG+TGG 0.653492 4:+85659039 None:intergenic
ACTCACGATGTCCTCTAGCG+AGG 0.698309 4:+85659004 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACTAATATATAACTTCAAT+TGG - Chr4:85658130-85658149 MsG0480023471.01.T01:CDS 15.0%
!! ATATTCCAGTATATTTAAAA+TGG + Chr4:85659025-85659044 None:intergenic 15.0%
!! TAATTTATAAACATCTTATG+TGG - Chr4:85657720-85657739 MsG0480023471.01.T01:intron 15.0%
!! TTTATGTGTTATATTATATG+AGG - Chr4:85658810-85658829 MsG0480023471.01.T01:CDS 15.0%
!! AAGAATGACAGAAAATTATA+GGG - Chr4:85658400-85658419 MsG0480023471.01.T01:intron 20.0%
!! AAGATTGATACAATATAAGA+AGG + Chr4:85658014-85658033 None:intergenic 20.0%
!! ATAGTTGCAAATAAAGAAAA+CGG + Chr4:85658903-85658922 None:intergenic 20.0%
!! ATATGATAAGCACTTAATTA+AGG + Chr4:85657420-85657439 None:intergenic 20.0%
!! TAAGAATGACAGAAAATTAT+AGG - Chr4:85658399-85658418 MsG0480023471.01.T01:intron 20.0%
!! TATAAGAAGATATAGAAAGT+GGG + Chr4:85657702-85657721 None:intergenic 20.0%
!! TTAATTGCTCAAAAATCAAA+AGG - Chr4:85657856-85657875 MsG0480023471.01.T01:intron 20.0%
!! TTATAAGAAGATATAGAAAG+TGG + Chr4:85657703-85657722 None:intergenic 20.0%
!!! ATTTGTAAGGCTTTAAAAAT+AGG + Chr4:85658456-85658475 None:intergenic 20.0%
!!! CAATAGAATAAGTTGAAATT+TGG - Chr4:85657264-85657283 MsG0480023471.01.T01:intron 20.0%
!!! TTATTCCTTATTCTTTTCAT+AGG - Chr4:85658490-85658509 MsG0480023471.01.T01:intron 20.0%
! AAAGATGTTACAGTTAATTC+CGG + Chr4:85658172-85658191 None:intergenic 25.0%
! AATCAATTAACTTTCTTCTG+TGG - Chr4:85657220-85657239 MsG0480023471.01.T01:intron 25.0%
! AGATGGATATTATTGGAATA+GGG - Chr4:85657095-85657114 MsG0480023471.01.T01:intron 25.0%
! ATTCAAGAGTAACATGAAAA+TGG + Chr4:85659127-85659146 None:intergenic 25.0%
! GTGTTATGTGTTCTAATATT+TGG - Chr4:85658753-85658772 MsG0480023471.01.T01:intron 25.0%
! TAACTTCAATTGGATTTGTA+TGG - Chr4:85658140-85658159 MsG0480023471.01.T01:CDS 25.0%
! TACCAAAAAATTATGATCTG+AGG + Chr4:85658328-85658347 None:intergenic 25.0%
! TATTTCTCCATTTAGGAAAA+TGG - Chr4:85658920-85658939 MsG0480023471.01.T01:intron 25.0%
! TGGAATATGAAGAATAACAT+TGG - Chr4:85659037-85659056 MsG0480023471.01.T01:CDS 25.0%
! TTAAATAAGTCGATACAAAC+AGG - Chr4:85657641-85657660 MsG0480023471.01.T01:intron 25.0%
!! AATCTTTTGAATCTTTCGTA+AGG - Chr4:85658028-85658047 MsG0480023471.01.T01:CDS 25.0%
!! ATTAGCCTATGAAAAGAATA+AGG + Chr4:85658498-85658517 None:intergenic 25.0%
!! TTTGCTTTGTTACTTATTTC+AGG - Chr4:85657817-85657836 MsG0480023471.01.T01:intron 25.0%
!!! ATTTGGTCTATTTTTCATCT+GGG - Chr4:85658950-85658969 MsG0480023471.01.T01:intron 25.0%
!!! CAGATCATAATTTTTTGGTA+GGG - Chr4:85658328-85658347 MsG0480023471.01.T01:intron 25.0%
!!! TCAGATCATAATTTTTTGGT+AGG - Chr4:85658327-85658346 MsG0480023471.01.T01:intron 25.0%
!!! TTCCTCAGATCATAATTTTT+TGG - Chr4:85658323-85658342 MsG0480023471.01.T01:intron 25.0%
AAGAAAACAATAACCTGAAG+AGG - Chr4:85658973-85658992 MsG0480023471.01.T01:intron 30.0%
AATCAGTGACATGAATTACA+AGG + Chr4:85656919-85656938 None:intergenic 30.0%
ATATTAGTTGCACACATCTT+TGG + Chr4:85658119-85658138 None:intergenic 30.0%
ATCAAATGTTCTCTTGTAGA+TGG - Chr4:85657979-85657998 MsG0480023471.01.T01:intron 30.0%
ATGGATATTATTGGAATAGG+GGG - Chr4:85657097-85657116 MsG0480023471.01.T01:intron 30.0%
CAGATGGATATTATTGGAAT+AGG - Chr4:85657094-85657113 MsG0480023471.01.T01:intron 30.0%
CATAATAGAGCAACTTTACA+AGG + Chr4:85657034-85657053 None:intergenic 30.0%
CTATTCCAATAATATCCATC+TGG + Chr4:85657096-85657115 None:intergenic 30.0%
GATGGATATTATTGGAATAG+GGG - Chr4:85657096-85657115 MsG0480023471.01.T01:intron 30.0%
TCTTTCAAAAGTTGTTAGCA+GGG - Chr4:85658776-85658795 MsG0480023471.01.T01:intron 30.0%
TTACCATCAGAGTTAGAATT+TGG + Chr4:85657193-85657212 None:intergenic 30.0%
TTCTTCTGTGGAGTTTAAAT+AGG - Chr4:85657232-85657251 MsG0480023471.01.T01:intron 30.0%
TTGATGCATGCAATCAATAA+TGG - Chr4:85657749-85657768 MsG0480023471.01.T01:CDS 30.0%
TTGCAGATAAGCAAAAATCT+CGG - Chr4:85659146-85659165 MsG0480023471.01.T01:CDS 30.0%
! AAAGCAGGTGTTTCTAATAA+TGG - Chr4:85658711-85658730 MsG0480023471.01.T01:intron 30.0%
! AATTTTTCACTTTCCTCTTC+AGG + Chr4:85658989-85659008 None:intergenic 30.0%
! CTGAATAATTTTCAACTCTG+TGG - Chr4:85658552-85658571 MsG0480023471.01.T01:intron 30.0%
! TCTTTTCATAGGCTAATCAT+GGG - Chr4:85658501-85658520 MsG0480023471.01.T01:intron 30.0%
! TGCAAACAGCATAATTTGTA+AGG + Chr4:85658469-85658488 None:intergenic 30.0%
! TGGCACCATTTTAAATATAC+TGG - Chr4:85659017-85659036 MsG0480023471.01.T01:CDS 30.0%
! TTCTTTTCATAGGCTAATCA+TGG - Chr4:85658500-85658519 MsG0480023471.01.T01:intron 30.0%
! TTGCAACTATTTCTCCATTT+AGG - Chr4:85658913-85658932 MsG0480023471.01.T01:intron 30.0%
! TTTTCTTACTGCTGTAAAGT+AGG + Chr4:85658378-85658397 None:intergenic 30.0%
!! ATTTTGAGAGTCACTTAAAG+AGG + Chr4:85658596-85658615 None:intergenic 30.0%
!! CATTTGGTCTATTTTTCATC+TGG - Chr4:85658949-85658968 MsG0480023471.01.T01:intron 30.0%
!!! TAGTTTTGAATGCCTTAAAG+AGG + Chr4:85657960-85657979 None:intergenic 30.0%
AAACATTATGGCATCTGAGA+AGG - Chr4:85658236-85658255 MsG0480023471.01.T01:intron 35.0%
AAAGTTGTTAGCAGGGTTTA+AGG - Chr4:85658783-85658802 MsG0480023471.01.T01:intron 35.0%
AAGAAATGAAACCATACCAC+TGG - Chr4:85658650-85658669 MsG0480023471.01.T01:intron 35.0%
AAGTGAAAAATTGCCTCTTC+TGG - Chr4:85658997-85659016 MsG0480023471.01.T01:intron 35.0%
AATCTTTGCTGAACTCAAAC+TGG - Chr4:85656814-85656833 MsG0480023471.01.T01:CDS 35.0%
AGGTTTCGATGACAGTAATT+TGG - Chr4:85657376-85657395 MsG0480023471.01.T01:intron 35.0%
ATTACAAGGTGAGAAGAATC+AGG + Chr4:85656905-85656924 None:intergenic 35.0%
ATTAGAACACATAACACACC+TGG + Chr4:85658750-85658769 None:intergenic 35.0%
ATTGATTGCATGCATCAAAG+AGG + Chr4:85657747-85657766 None:intergenic 35.0%
CACAATTGTTCAACTCGATT+TGG + Chr4:85658270-85658289 None:intergenic 35.0%
GCATGAACATGGAACAAAAA+AGG + Chr4:85656881-85656900 None:intergenic 35.0%
GGTCATCATTCTGTTAGTAT+GGG - Chr4:85658421-85658440 MsG0480023471.01.T01:intron 35.0%
GGTTTCATTTCTTTCCTGAT+AGG + Chr4:85658643-85658662 None:intergenic 35.0%
GGTTTCGATGACAGTAATTT+GGG - Chr4:85657377-85657396 MsG0480023471.01.T01:intron 35.0%
GTAAGGTCTTGATTTCTGTT+TGG - Chr4:85658045-85658064 MsG0480023471.01.T01:CDS 35.0%
GTCTTTCAAAAGTTGTTAGC+AGG - Chr4:85658775-85658794 MsG0480023471.01.T01:intron 35.0%
TGCAGATAAGCAAAAATCTC+GGG - Chr4:85659147-85659166 MsG0480023471.01.T01:CDS 35.0%
TGTGCAGTTGACAAACATTA+TGG - Chr4:85658224-85658243 MsG0480023471.01.T01:intron 35.0%
TTTATCCTTGAGCCTCTTTA+AGG - Chr4:85657945-85657964 MsG0480023471.01.T01:intron 35.0%
! AAGCTTTTCTACAGAGCTTA+TGG - Chr4:85657525-85657544 MsG0480023471.01.T01:intron 35.0%
! ACACTCTTTTTCTACCTATC+AGG - Chr4:85658626-85658645 MsG0480023471.01.T01:intron 35.0%
! AGTTTGAGTTCAGCAAAGAT+TGG + Chr4:85656815-85656834 None:intergenic 35.0%
! ATTTAAAATGGTGCCAGAAG+AGG + Chr4:85659013-85659032 None:intergenic 35.0%
! CACACGATTCTTAACTTTTG+TGG + Chr4:85659182-85659201 None:intergenic 35.0%
!! ATTTGGTGTATATCCTCTTG+TGG - Chr4:85657281-85657300 MsG0480023471.01.T01:intron 35.0%
!! TCCATCGATTTAAGTAGCAA+TGG - Chr4:85656772-85656791 MsG0480023471.01.T01:CDS 35.0%
!! TGTAGTGTTGTTTGTTGTAC+AGG - Chr4:85659075-85659094 MsG0480023471.01.T01:CDS 35.0%
!!! ATTTTTTGGTAGGGATTCCT+TGG - Chr4:85658337-85658356 MsG0480023471.01.T01:intron 35.0%
AAGCAAAAATCTCGGGATAC+AGG - Chr4:85659154-85659173 MsG0480023471.01.T01:CDS 40.0%
AGGAAAATGGTGACTGCATT+TGG - Chr4:85658933-85658952 MsG0480023471.01.T01:intron 40.0%
AGTCGATACAAACAGGTCAT+TGG - Chr4:85657648-85657667 MsG0480023471.01.T01:intron 40.0%
ATATCCTCTTGTGGAATGCA+TGG - Chr4:85657290-85657309 MsG0480023471.01.T01:intron 40.0%
ATGGCATACTGTTTGTCTTG+AGG - Chr4:85657768-85657787 MsG0480023471.01.T01:CDS 40.0%
ATTAGTGACTCAGAGAAAGC+AGG - Chr4:85658696-85658715 MsG0480023471.01.T01:intron 40.0%
CATTCCTCTTACCAAACTAG+TGG - Chr4:85657894-85657913 MsG0480023471.01.T01:intron 40.0%
GAGCCAAATTCTAACTCTGA+TGG - Chr4:85657187-85657206 MsG0480023471.01.T01:intron 40.0%
GAGTTGAACAATTGTGCTTG+GGG - Chr4:85658274-85658293 MsG0480023471.01.T01:intron 40.0%
GGAATGCCAAATCAATTCCA+AGG + Chr4:85658357-85658376 None:intergenic 40.0%
GGATGAGATTGACAAGTATG+AGG + Chr4:85656851-85656870 None:intergenic 40.0%
GGGATTCCTTGGAATTGATT+TGG - Chr4:85658348-85658367 MsG0480023471.01.T01:intron 40.0%
GGGTCATCATTCTGTTAGTA+TGG - Chr4:85658420-85658439 MsG0480023471.01.T01:intron 40.0%
GTAAGAGGAATGGTGATTAG+AGG + Chr4:85657886-85657905 None:intergenic 40.0%
TCGCTCCAGATGGATATTAT+TGG - Chr4:85657088-85657107 MsG0480023471.01.T01:intron 40.0%
TGAGATTGACAAGTATGAGG+TGG + Chr4:85656848-85656867 None:intergenic 40.0%
TGATTTCCACTCTGACACTT+GGG - Chr4:85656934-85656953 MsG0480023471.01.T01:intron 40.0%
TGTCTATGACACTTCCATCT+TGG - Chr4:85657338-85657357 MsG0480023471.01.T01:intron 40.0%
TTATTTACCTTCGCTCCAGA+TGG - Chr4:85657078-85657097 MsG0480023471.01.T01:intron 40.0%
TTCCATCTTGGTCACTCTTA+TGG - Chr4:85657350-85657369 MsG0480023471.01.T01:intron 40.0%
! AAGAATCAGGAGCATGAACA+TGG + Chr4:85656892-85656911 None:intergenic 40.0%
! ATAATATCCATCTGGAGCGA+AGG + Chr4:85657088-85657107 None:intergenic 40.0%
! CACTAGTTTGGTAAGAGGAA+TGG + Chr4:85657896-85657915 None:intergenic 40.0%
! CGAGTTGAACAATTGTGCTT+GGG - Chr4:85658273-85658292 MsG0480023471.01.T01:intron 40.0%
! CTTCAGATCTTCTGACTTGA+GGG + Chr4:85657158-85657177 None:intergenic 40.0%
! GCAGTCACCATTTTCCTAAA+TGG + Chr4:85658930-85658949 None:intergenic 40.0%
! GCCATTGCTACTTAAATCGA+TGG + Chr4:85656776-85656795 None:intergenic 40.0%
! TGCTACTTAAATCGATGGAG+TGG + Chr4:85656771-85656790 None:intergenic 40.0%
!! TCGAGTTGAACAATTGTGCT+TGG - Chr4:85658272-85658291 MsG0480023471.01.T01:intron 40.0%
AAACCATACCACTGGATCGA+CGG - Chr4:85658658-85658677 MsG0480023471.01.T01:intron 45.0%
AATGGAGCACAAAACTCTCC+AGG - Chr4:85658729-85658748 MsG0480023471.01.T01:intron 45.0%
ATCTCCATGCATTCCACAAG+AGG + Chr4:85657297-85657316 None:intergenic 45.0%
CTCCATAAGAGTGACCAAGA+TGG + Chr4:85657355-85657374 None:intergenic 45.0%
CTGATTTCCACTCTGACACT+TGG - Chr4:85656933-85656952 MsG0480023471.01.T01:intron 45.0%
CTTGGTCACTCTTATGGAGA+AGG - Chr4:85657356-85657375 MsG0480023471.01.T01:intron 45.0%
GAATGCCTTAAAGAGGCTCA+AGG + Chr4:85657953-85657972 None:intergenic 45.0%
TCTTTCAGTTTCCTCGCTAG+AGG - Chr4:85656964-85656983 MsG0480023471.01.T01:intron 45.0%
TGGAACAAAAAAGGACGCGA+AGG + Chr4:85656872-85656891 None:intergenic 45.0%
TGGATTTGTATGGTCTGTGC+CGG - Chr4:85658150-85658169 MsG0480023471.01.T01:intron 45.0%
TTATTGGAATAGGGGGCTCT+TGG - Chr4:85657104-85657123 MsG0480023471.01.T01:intron 45.0%
TTGCTGAACTCAAACTGGAG+TGG - Chr4:85656819-85656838 MsG0480023471.01.T01:CDS 45.0%
! GAGTCCACTAGTTTGGTAAG+AGG + Chr4:85657901-85657920 None:intergenic 45.0%
! GCTTCAGATCTTCTGACTTG+AGG + Chr4:85657159-85657178 None:intergenic 45.0%
! AACTTTTGCCGTCGATCCAG+TGG + Chr4:85658669-85658688 None:intergenic 50.0%
! AGCACTGGAGTCCACTAGTT+TGG + Chr4:85657908-85657927 None:intergenic 50.0%
AACACTTGCCTGCGATGTGC+TGG + Chr4:85659208-85659227 None:intergenic 55.0%
ACTCACGATGTCCTCTAGCG+AGG + Chr4:85656978-85656997 None:intergenic 55.0%
GAGCTTCCCAAGTGTCAGAG+TGG + Chr4:85656943-85656962 None:intergenic 55.0%
TGACACAGCTTCGCTAGCAC+TGG + Chr4:85657923-85657942 None:intergenic 55.0%
TTGCCGTCGATCCAGTGGTA+TGG + Chr4:85658664-85658683 None:intergenic 55.0%
! ACAGAGCGTTCTTGCACTGG+TGG + Chr4:85658207-85658226 None:intergenic 55.0%
! TGCACAGAGCGTTCTTGCAC+TGG + Chr4:85658210-85658229 None:intergenic 55.0%
GTGTGATGCCAGCACATCGC+AGG - Chr4:85659197-85659216 MsG0480023471.01.T01:exon 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 85656752 85659249 85656752 ID=MsG0480023471.01;Name=MsG0480023471.01
Chr4 mRNA 85656752 85659249 85656752 ID=MsG0480023471.01.T01;Parent=MsG0480023471.01;Name=MsG0480023471.01.T01;_AED=0.46;_eAED=0.52;_QI=39|0.6|0.66|1|1|1|6|0|219
Chr4 exon 85656752 85656849 85656752 ID=MsG0480023471.01.T01:exon:5914;Parent=MsG0480023471.01.T01
Chr4 exon 85657613 85657652 85657613 ID=MsG0480023471.01.T01:exon:5913;Parent=MsG0480023471.01.T01
Chr4 exon 85657744 85657790 85657744 ID=MsG0480023471.01.T01:exon:5912;Parent=MsG0480023471.01.T01
Chr4 exon 85658001 85658162 85658001 ID=MsG0480023471.01.T01:exon:5911;Parent=MsG0480023471.01.T01
Chr4 exon 85658793 85658904 85658793 ID=MsG0480023471.01.T01:exon:5910;Parent=MsG0480023471.01.T01
Chr4 exon 85659010 85659249 85659010 ID=MsG0480023471.01.T01:exon:5909;Parent=MsG0480023471.01.T01
Chr4 five_prime_UTR 85659211 85659249 85659211 ID=MsG0480023471.01.T01:five_prime_utr;Parent=MsG0480023471.01.T01
Chr4 CDS 85659010 85659210 85659010 ID=MsG0480023471.01.T01:cds;Parent=MsG0480023471.01.T01
Chr4 CDS 85658793 85658904 85658793 ID=MsG0480023471.01.T01:cds;Parent=MsG0480023471.01.T01
Chr4 CDS 85658001 85658162 85658001 ID=MsG0480023471.01.T01:cds;Parent=MsG0480023471.01.T01
Chr4 CDS 85657744 85657790 85657744 ID=MsG0480023471.01.T01:cds;Parent=MsG0480023471.01.T01
Chr4 CDS 85657613 85657652 85657613 ID=MsG0480023471.01.T01:cds;Parent=MsG0480023471.01.T01
Chr4 CDS 85656752 85656849 85656752 ID=MsG0480023471.01.T01:cds;Parent=MsG0480023471.01.T01
Gene Sequence

>MsG0480023471.01.T01

ATGGCGTTTCAAGACTTTGAACCAATCTTTGCTGAACTCAAACTGGAGTGGAAACCACCTCATACTTGTCAATCTCATCCTTCGCGTCCTTTTTTGTTCCATGTTCATGCTCCTGATTCTTCTCACCTTGTAATTCATGTCACTGATTTCCACTCTGACACTTGGGAAGCTCATCTTTCAGTTTCCTCGCTAGAGGACATCATGGATATTATTGGAATAGGGGGCTCTTGGTCTGAGTTTGCTGATTATTTTGTAAATTCCCTCAAGTCAGAAGATCTGAAGCTCGTTCTAGAGCCAAATTCTAACTCTGATGGTGTTAGTTCTGCTAAATTAATTGCTCAAAAATCAAAAGGAATGCCTCTAATCACCATTCCTCTTACCAAACTAGTGGACTCCAGTGCTAGCGAAGCTGTGTCAAATTTATCCTTGAGCCTCTTTAAGGCATTCAAAACTATCAAATGTTCTCTTGTAGATGTGCAAGAACGCTCTGTGCAGTTGACAAACATTATGGCATCTGAGAAGGGATTCCTTGGAATTGATTTGGCATTCCTACTTTACAGCAATAAGCAAAAATCTCGGGATACAGGCACCACAAAAGTTAAGAATCGTGTGATGCCAGCACATCGCAGGCAAGTGTTCCTACTATATTACAAACCTTAA
Protein sequence

>MsG0480023471.01.T01

MAFQDFEPIFAELKLEWKPPHTCQSHPSRPFLFHVHAPDSSHLVIHVTDFHSDTWEAHLSVSSLEDIMDIIGIGGSWSEFADYFVNSLKSEDLKLVLEPNSNSDGVSSAKLIAQKSKGMPLITIPLTKLVDSSASEAVSNLSLSLFKAFKTIKCSLVDVQERSVQLTNIMASEKGFLGIDLAFLLYSNKQKSRDTGTTKVKNRVMPAHRRQVFLLYYKP*