Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012567.01.T01 | XP_003598994.1 | 87.734 | 481 | 49 | 4 | 1 | 478 | 1 | 474 | 0 | 824 |
| MsG0380012567.01.T01 | XP_003598994.1 | 93.534 | 232 | 15 | 0 | 531 | 762 | 434 | 665 | 1.63E-145 | 452 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047884.01 | MsG0380012567.01 | 0.809669 | 1.688256e-50 | 9.973584e-48 |
| MsG0080047885.01 | MsG0380012567.01 | 0.840552 | 8.118320e-58 | 1.160519e-54 |
| MsG0080048065.01 | MsG0380012567.01 | 0.808263 | 3.374657e-50 | 1.920812e-47 |
| MsG0080048660.01 | MsG0380012567.01 | 0.841007 | 6.167072e-58 | 8.940339e-55 |
| MsG0080048727.01 | MsG0380012567.01 | 0.872207 | 3.797220e-67 | 1.575306e-63 |
| MsG0080048748.01 | MsG0380012567.01 | 0.863889 | 1.802952e-64 | 5.552645e-61 |
| MsG0080048773.01 | MsG0380012567.01 | 0.869424 | 3.129560e-66 | 1.174086e-62 |
| MsG0080048780.01 | MsG0380012567.01 | 0.821625 | 3.654608e-53 | 2.988768e-50 |
| MsG0080048802.01 | MsG0380012567.01 | 0.818263 | 2.147777e-52 | 1.599842e-49 |
| MsG0080048820.01 | MsG0380012567.01 | 0.842327 | 2.766539e-58 | 4.180221e-55 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012567.01.T01 | MTR_3g025870 | 87.734 | 481 | 49 | 4 | 1 | 478 | 1 | 474 | 0.0 | 824 |
| MsG0380012567.01.T01 | MTR_3g025870 | 93.534 | 232 | 15 | 0 | 531 | 762 | 434 | 665 | 1.97e-149 | 452 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012567.01.T01 | AT5G61040 | 36.466 | 266 | 120 | 7 | 533 | 759 | 332 | 587 | 6.19e-33 | 135 |
| MsG0380012567.01.T01 | AT5G61040 | 28.228 | 457 | 192 | 15 | 1 | 443 | 1 | 335 | 1.29e-27 | 119 |
| MsG0380012567.01.T01 | AT5G08010 | 31.852 | 270 | 152 | 4 | 520 | 757 | 292 | 561 | 3.59e-26 | 114 |
| MsG0380012567.01.T01 | AT5G08010 | 28.080 | 349 | 157 | 13 | 1 | 347 | 1 | 257 | 7.46e-19 | 91.3 |
Find 163 sgRNAs with CRISPR-Local
Find 314 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACGTTTCGTTGAGAGAA+AGG | 0.374754 | 3:+19897318 | None:intergenic |
| AAAATAGCCAGATGAAAGTA+AGG | 0.469262 | 3:-19894244 | MsG0380012567.01.T01:CDS |
| AAAATTCTTGCAGATTGATC+CGG | 0.500918 | 3:-19894450 | MsG0380012567.01.T01:CDS |
| AAACGTAAACTCAGTTCATG+CGG | 0.690091 | 3:+19894326 | None:intergenic |
| AAAGACATATTCTTCCATTT+TGG | 0.197205 | 3:+19897225 | None:intergenic |
| AACTTTAGGGATGGTGCAGA+TGG | 0.495448 | 3:-19897439 | MsG0380012567.01.T01:CDS |
| AAGAAGAGGAGAACCTTTCT+TGG | 0.349044 | 3:+19893830 | None:intergenic |
| AAGCTGAACTTGAGAGGTTA+GGG | 0.400444 | 3:-19895383 | MsG0380012567.01.T01:CDS |
| AAGGAATCTTCTCCCACAAG+AGG | 0.611867 | 3:-19894188 | MsG0380012567.01.T01:CDS |
| AAGGTCCGCTTTCGCATGAT+TGG | 0.372235 | 3:-19893995 | MsG0380012567.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATAGCCAGATGAAAGTA+AGG | - | Chr3:19898452-19898471 | MsG0380012567.01.T01:intron | 30.0% | |
| AAAATTCTTGCAGATTGATC+CGG | - | Chr3:19898246-19898265 | MsG0380012567.01.T01:five_prime_UTR | 30.0% | |
| AAACACAAAAATGTAGTTGG+AGG | - | Chr3:19895632-19895651 | MsG0380012567.01.T01:CDS | 30.0% | |
| AACACAAAAATGTAGTTGGA+GGG | - | Chr3:19895633-19895652 | MsG0380012567.01.T01:CDS | 30.0% | |
| ACCTGATAGATTGAGAATTA+AGG | + | Chr3:19898578-19898597 | None:intergenic | 30.0% | |
| ATCAATAAGTTTCCTCTTGT+GGG | + | Chr3:19898523-19898542 | None:intergenic | 30.0% | |
| ATTGTTGAAAGAACCAAGAA+AGG | - | Chr3:19898853-19898872 | MsG0380012567.01.T01:five_prime_UTR | 30.0% | |
| CAATATTGTTAGTGTGACAA+TGG | - | Chr3:19898210-19898229 | MsG0380012567.01.T01:five_prime_UTR | 30.0% | |
| CACCATATGATGATAACTTT+AGG | - | Chr3:19895243-19895262 | MsG0380012567.01.T01:intron | 30.0% | |
| CAGACAAACATTGAAGATAA+CGG | - | Chr3:19893791-19893810 | MsG0380012567.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 19893776 | 19898942 | 19893776 | ID=MsG0380012567.01;Name=MsG0380012567.01 |
| Chr3 | mRNA | 19893776 | 19898942 | 19893776 | ID=MsG0380012567.01.T01;Parent=MsG0380012567.01;Name=MsG0380012567.01.T01;_AED=0.24;_eAED=0.30;_QI=562|0.66|0.5|1|0.66|0.5|4|0|762 |
| Chr3 | exon | 19898824 | 19898942 | 19898824 | ID=MsG0380012567.01.T01:exon:3759;Parent=MsG0380012567.01.T01 |
| Chr3 | exon | 19896936 | 19898282 | 19896936 | ID=MsG0380012567.01.T01:exon:3760;Parent=MsG0380012567.01.T01 |
| Chr3 | exon | 19895307 | 19895753 | 19895307 | ID=MsG0380012567.01.T01:exon:3761;Parent=MsG0380012567.01.T01 |
| Chr3 | exon | 19893776 | 19894713 | 19893776 | ID=MsG0380012567.01.T01:exon:3762;Parent=MsG0380012567.01.T01 |
| Chr3 | five_prime_UTR | 19898824 | 19898942 | 19898824 | ID=MsG0380012567.01.T01:five_prime_utr;Parent=MsG0380012567.01.T01 |
| Chr3 | five_prime_UTR | 19897840 | 19898282 | 19897840 | ID=MsG0380012567.01.T01:five_prime_utr;Parent=MsG0380012567.01.T01 |
| Chr3 | CDS | 19896936 | 19897839 | 19896936 | ID=MsG0380012567.01.T01:cds;Parent=MsG0380012567.01.T01 |
| Chr3 | CDS | 19895307 | 19895753 | 19895307 | ID=MsG0380012567.01.T01:cds;Parent=MsG0380012567.01.T01 |
| Chr3 | CDS | 19893776 | 19894713 | 19893776 | ID=MsG0380012567.01.T01:cds;Parent=MsG0380012567.01.T01 |
| Gene Sequence |
>MsG0380012567.01.T01 ATGGATTTGTGGCTTGCAGCTGCTTTTACTGGAGCTGGTTATTTAGCTAAATATTGGAAAAAGAGTTTGGAAAATGGTGATACCTCTTATCATTTGTCTTCAGAGGATTCGAATTTTGAGAAAGCCGAGTCCTTTAGCAGCTCCTTAACCTCCCTCAAACAACCCCGGAGACGTGAAAAGGGAAAAGATGTTTTTTTAGATAGAAGGGGTTCGGATGAGATGTTCTCAGATGCGAATTCCTTGGATGGTCCTTCAACAGGAGACGTGGCTTCTTATAGAGGGTTCAATTGTGAAAAAGTAAGACATTTTCAGAACTATAATGAGAGTGATTTTCTATCGATATCGAACTTAGCAGTGCCATTATCACCATATGATGATAACTTTAGGGATGGTGCAGATGGAAATGAGCAAAATACGAGTAGTTTTGGCAATCATGGGTTCTTTCGTCCTGAGTTTCCTGCCAAATTGATTCCTATCCACAACTCTTTCGGACACAAAACCTTTCTCTCAACGAAACGTTTTCCCGAGCATGTTAGTAGACCATTAAATTCCTTAGAAAGTTGTTTCATGGCTCAGCTATATAAAGAGCATGCCAAAATGGAAGAATATGTCTTTAGTCCTCTTTCATCACCATCTACAACAAGGTTATTTCGTGTTAGCAATGGTAGCCGAATACTGAATAGAGAAAATTGTAGTTTGATCAGTGCATTGACTGGAAGTAAGGATTATAAGCTGCATAAAGCTGGTCGAGAAAAACACAAAAATGTAGTTGGAGGGGTTCCTTCCTTGCCCAAAATTGGATCTTTAAATGATACCAAGAAGATGAAACTTGATGCAGTTGATGGACGAAGTCGAAGGTCAAGCTTTTCTGATGACTTGTTTAGTGGAAAACTCACTCAATATGATCCGGCGTTTCTCTTCAGTCTTGGGATTTCTCTTGGGATAATAACTTCCAACATGGCAAATAAAAGAGAAATAGTCAAGATAAGAGAACTGTTGAAGCAGAATGAGAACTTGGTTCAAGATCTACAAGACGAACTCGATATGAAAGATTCGATGACAGTGAAGGAGTTACAAAATGAAAATTATGGTTCACAAGATACGTGTGATCATTCCTTCAATGGTAAGGAGCTACATGAATTTTCCCCTGAAAAGCATATTGATAGCTCTCCCAGAGTAGAATGCAAAGAATCATATGATAAAAAGGAAGAACAGAGTTCAGAGTCTATGAGCAAAATTGAAGCTGAGCTTGAAGCTGAACTTGAGAGGTTAGGGTTGGACATGAATGCGTCAAGCCTGGATAGGAAATTATCCGAGCTTGTTGAGGTAAGCATAACTGTCACATTTTCCGATATTGGAATTGTGGCAGTAACTGAAAATTTCACAGCTATAGATGCTAGTTTAAGTTTTCTCTGTCCAATGTTGCATGCCTTATTTTGCTTTCAAGTTCTACCATGTATGTTTGTTTTGATAGCCATACAAAGTTTCTTTTTCCAGTGTAAAGTAAATCTCATATTTTGCTCCATAGCTATGTTTGGTTCAAGTAATCAAGTTAACAATATTGTTAGTGTGACAATGGGTGTTAATGGTGCAAAATTCTTGCAGATTGATCCGGAGTTTGTAGCAGATTTTGCGCAAGGTGAGTTTCGAGCTGACACGGCTAGTGGAGCAGATGCCAATGTCACCACACCTCTTCCTGCAAACTATGCAGTTTCACCGCATGAACTGAGTTTACGTTTGCACGAAGTCATACAAAACCAACTCGAAACACGAGTGAAAGAGCTTGAGATAGCCCTTGAAAATAGCCAGATGAAAGTAAGGTCTTTGGAAACTGAACATGATGGTAGTTTCCAAAAGGAATCTTCTCCCACAAGAGGAAACTTATTGATGACTCATGAAGATTGCAACACTATGTCCCAGCCCTTAATTCTCAATCTATCAGGTGAAGCTCTTGAAGCCTACAATGAAACCTACGAGGAATTAATAAATATGAATGATTCCGAAGACAATTCACCATCAGCCATCATTCATGATAGTGACGACAAAGAAGGTCCGCTTTCGCATGATTGGAATGCAACAGTTTTTGAATACGATTTCGCGACAATGTTGGAAGGGCAAAGCTCTAGTGAATTGAATGTTAGTGGAGATGAAAGTTGTGAATGTGATAGTGAGGAGGTGGAGAGGCTATTGATAAGGCAAATTGTTGAAAGAACCAAGAAAGGTTCTCCTCTTCTTCAAAATGCAAAAAAGATATTGTATTCTATGGATGAAGATGAACAACAACATTGA |
| Protein sequence |
>MsG0380012567.01.T01 MDLWLAAAFTGAGYLAKYWKKSLENGDTSYHLSSEDSNFEKAESFSSSLTSLKQPRRREKGKDVFLDRRGSDEMFSDANSLDGPSTGDVASYRGFNCEKVRHFQNYNESDFLSISNLAVPLSPYDDNFRDGADGNEQNTSSFGNHGFFRPEFPAKLIPIHNSFGHKTFLSTKRFPEHVSRPLNSLESCFMAQLYKEHAKMEEYVFSPLSSPSTTRLFRVSNGSRILNRENCSLISALTGSKDYKLHKAGREKHKNVVGGVPSLPKIGSLNDTKKMKLDAVDGRSRRSSFSDDLFSGKLTQYDPAFLFSLGISLGIITSNMANKREIVKIRELLKQNENLVQDLQDELDMKDSMTVKELQNENYGSQDTCDHSFNGKELHEFSPEKHIDSSPRVECKESYDKKEEQSSESMSKIEAELEAELERLGLDMNASSLDRKLSELVEVSITVTFSDIGIVAVTENFTAIDASLSFLCPMLHALFCFQVLPCMFVLIAIQSFFFQCKVNLIFCSIAMFGSSNQVNNIVSVTMGVNGAKFLQIDPEFVADFAQGEFRADTASGADANVTTPLPANYAVSPHELSLRLHEVIQNQLETRVKELEIALENSQMKVRSLETEHDGSFQKESSPTRGNLLMTHEDCNTMSQPLILNLSGEALEAYNETYEELINMNDSEDNSPSAIIHDSDDKEGPLSHDWNATVFEYDFATMLEGQSSSELNVSGDESCECDSEEVERLLIRQIVERTKKGSPLLQNAKKILYSMDEDEQQH* |
