Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037291.01.T01 | GFZ07213.1 | 89.655 | 58 | 6 | 0 | 13 | 70 | 12 | 69 | 3.05E-29 | 109 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037291.01.T01 | O04834 | 86.207 | 58 | 8 | 0 | 13 | 70 | 87 | 144 | 1.15E-28 | 103 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037291.01.T01 | A0A7J0G8V1 | 89.655 | 58 | 6 | 0 | 13 | 70 | 12 | 69 | 1.46e-29 | 109 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000091.01 | MsG0780037291.01 | 0.811211 | 7.841127e-51 | 4.824866e-48 |
| MsG0180000787.01 | MsG0780037291.01 | 0.811560 | 6.587698e-51 | 4.090947e-48 |
| MsG0180004765.01 | MsG0780037291.01 | 0.811881 | 5.608818e-51 | 3.512693e-48 |
| MsG0180006251.01 | MsG0780037291.01 | 0.804921 | 1.713038e-49 | 8.943980e-47 |
| MsG0280010599.01 | MsG0780037291.01 | 0.812268 | 4.618827e-51 | 2.923388e-48 |
| MsG0280010916.01 | MsG0780037291.01 | 0.811630 | 6.358345e-51 | 3.955659e-48 |
| MsG0380012005.01 | MsG0780037291.01 | 0.801695 | 7.974710e-49 | 3.833096e-46 |
| MsG0380014727.01 | MsG0780037291.01 | 0.813568 | 2.395981e-51 | 1.570229e-48 |
| MsG0380014866.01 | MsG0780037291.01 | 0.804707 | 1.898331e-49 | 9.856125e-47 |
| MsG0680033465.01 | MsG0780037291.01 | 0.801666 | 8.085356e-49 | 3.883508e-46 |
| MsG0680034739.01 | MsG0780037291.01 | 0.806299 | 8.801589e-50 | 4.760310e-47 |
| MsG0680035580.01 | MsG0780037291.01 | 0.801337 | 9.443816e-49 | 4.498532e-46 |
| MsG0480020024.01 | MsG0780037291.01 | 0.817112 | 3.903585e-52 | 2.817516e-49 |
| MsG0480023251.01 | MsG0780037291.01 | 0.812704 | 3.707566e-51 | 2.373927e-48 |
| MsG0480023488.01 | MsG0780037291.01 | 0.804715 | 1.891426e-49 | 9.822391e-47 |
| MsG0380016740.01 | MsG0780037291.01 | 0.818146 | 2.282757e-52 | 1.694970e-49 |
| MsG0380017293.01 | MsG0780037291.01 | 0.808541 | 2.943624e-50 | 1.687647e-47 |
| MsG0380017498.01 | MsG0780037291.01 | 0.815029 | 1.139823e-51 | 7.771154e-49 |
| MsG0480018251.01 | MsG0780037291.01 | 0.813363 | 2.659234e-51 | 1.733103e-48 |
| MsG0480018402.01 | MsG0780037291.01 | 0.815877 | 7.381764e-52 | 5.150710e-49 |
| MsG0580029376.01 | MsG0780037291.01 | 0.808353 | 3.228858e-50 | 1.842168e-47 |
| MsG0680030616.01 | MsG0780037291.01 | 0.815137 | 1.078470e-51 | 7.373960e-49 |
| MsG0280006491.01 | MsG0780037291.01 | 0.815549 | 8.735009e-52 | 6.040640e-49 |
| MsG0280006601.01 | MsG0780037291.01 | 0.817622 | 2.997836e-52 | 2.194128e-49 |
| MsG0280007547.01 | MsG0780037291.01 | 0.803590 | 3.241796e-49 | 1.635533e-46 |
| MsG0280008112.01 | MsG0780037291.01 | 0.800554 | 1.364712e-48 | 6.373243e-46 |
| MsG0280008113.01 | MsG0780037291.01 | 0.814385 | 1.583081e-51 | 1.060755e-48 |
| MsG0280008894.01 | MsG0780037291.01 | 0.802927 | 4.447448e-49 | 2.205876e-46 |
| MsG0280010184.01 | MsG0780037291.01 | 0.805533 | 1.275247e-49 | 6.762994e-47 |
| MsG0280010517.01 | MsG0780037291.01 | 0.800510 | 1.393073e-48 | 6.498357e-46 |
| MsG0780036117.01 | MsG0780037291.01 | 0.800222 | 1.594824e-48 | 7.385261e-46 |
| MsG0780036128.01 | MsG0780037291.01 | 0.807195 | 5.692107e-50 | 3.150420e-47 |
| MsG0780036602.01 | MsG0780037291.01 | 0.805945 | 1.044544e-49 | 5.598815e-47 |
| MsG0780037291.01 | MsG0780037292.01 | 0.824644 | 7.218736e-54 | 6.432862e-51 |
| MsG0780037291.01 | MsG0780038439.01 | 0.807559 | 4.763828e-50 | 2.661663e-47 |
| MsG0780037291.01 | MsG0780038644.01 | 0.800519 | 1.387559e-48 | 6.474098e-46 |
| MsG0780037291.01 | MsG0880042700.01 | 0.803961 | 2.714692e-49 | 1.382713e-46 |
| MsG0780037291.01 | MsG0880044573.01 | 0.818155 | 2.271725e-52 | 1.687236e-49 |
| MsG0780037291.01 | MsG0880044728.01 | 0.801124 | 1.044005e-48 | 4.946622e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037291.01.T01 | MTR_4g121860 | 93.103 | 58 | 4 | 0 | 13 | 70 | 87 | 144 | 1.95e-32 | 110 |
| MsG0780037291.01.T01 | MTR_2g034640 | 87.931 | 58 | 7 | 0 | 13 | 70 | 87 | 144 | 1.97e-30 | 105 |
| MsG0780037291.01.T01 | MTR_7g112090 | 87.719 | 57 | 7 | 0 | 13 | 69 | 87 | 143 | 8.15e-30 | 104 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037291.01.T01 | AT3G62560 | 87.931 | 58 | 7 | 0 | 13 | 70 | 87 | 144 | 1.52e-30 | 106 |
| MsG0780037291.01.T01 | AT1G02620 | 84.483 | 58 | 9 | 0 | 13 | 70 | 16 | 73 | 1.09e-29 | 102 |
| MsG0780037291.01.T01 | AT4G02080 | 86.207 | 58 | 8 | 0 | 13 | 70 | 87 | 144 | 1.17e-29 | 103 |
| MsG0780037291.01.T01 | AT1G56330 | 82.759 | 58 | 10 | 0 | 13 | 70 | 87 | 144 | 9.16e-29 | 101 |
| MsG0780037291.01.T01 | AT1G09180 | 77.586 | 58 | 13 | 0 | 13 | 70 | 87 | 144 | 4.10e-26 | 94.7 |
| MsG0780037291.01.T01 | AT5G37680 | 48.214 | 56 | 29 | 0 | 13 | 68 | 40 | 95 | 8.28e-11 | 54.3 |
| MsG0780037291.01.T01 | AT3G49870 | 50.909 | 55 | 27 | 0 | 14 | 68 | 69 | 123 | 8.55e-11 | 54.7 |
Find 12 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAGCTCTTTCTTTGATTC+TGG | 0.177361 | 7:-23207296 | None:intergenic |
| ATGATGAATTACGTTTCATT+TGG | 0.310555 | 7:+23207402 | MsG0780037291.01.T01:CDS |
| AATGTACCTTTCCTTATCTT+AGG | 0.333519 | 7:+23207347 | MsG0780037291.01.T01:CDS |
| TGATGAATTACGTTTCATTT+GGG | 0.375075 | 7:+23207403 | MsG0780037291.01.T01:CDS |
| CAATCTTGTTTCCTAAGATA+AGG | 0.427363 | 7:-23207358 | None:intergenic |
| TCATCATCTGATGCAGCATA+AGG | 0.468650 | 7:-23207386 | None:intergenic |
| TCCAGAATCAAAGAAAGAGC+TGG | 0.549416 | 7:+23207295 | MsG0780037291.01.T01:CDS |
| CTTCTTGCTGATGAGTCACT+AGG | 0.554172 | 7:+23207323 | MsG0780037291.01.T01:CDS |
| GGTGGATGCTGTAGTCTACC+TGG | 0.579563 | 7:+23207247 | MsG0780037291.01.T01:intron |
| CCTTGTCAAATGCATCAACC+AGG | 0.583815 | 7:-23207265 | None:intergenic |
| TTGTTTCCTAAGATAAGGAA+AGG | 0.590342 | 7:-23207353 | None:intergenic |
| CCTGGTTGATGCATTTGACA+AGG | 0.590610 | 7:+23207265 | MsG0780037291.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTTAATTGTAGATTTTATA+TGG | + | Chr7:23207220-23207239 | MsG0780037291.01.T01:intron | 15.0% |
| ! | ATGATGAATTACGTTTCATT+TGG | + | Chr7:23207402-23207421 | MsG0780037291.01.T01:CDS | 25.0% |
| ! | TGATGAATTACGTTTCATTT+GGG | + | Chr7:23207403-23207422 | MsG0780037291.01.T01:CDS | 25.0% |
| !! | TTGTAGATTTTATATGGTAC+AGG | + | Chr7:23207226-23207245 | MsG0780037291.01.T01:intron | 25.0% |
| AATGTACCTTTCCTTATCTT+AGG | + | Chr7:23207347-23207366 | MsG0780037291.01.T01:CDS | 30.0% | |
| CAATGCATATGATTACGTTA+AGG | - | Chr7:23207150-23207169 | None:intergenic | 30.0% | |
| TTGTTTCCTAAGATAAGGAA+AGG | - | Chr7:23207356-23207375 | None:intergenic | 30.0% | |
| ! | CAATCTTGTTTCCTAAGATA+AGG | - | Chr7:23207361-23207380 | None:intergenic | 30.0% |
| ! | TAGATTTTATATGGTACAGG+TGG | + | Chr7:23207229-23207248 | MsG0780037291.01.T01:intron | 30.0% |
| AGCGTGAATGTAATCATGTG+TGG | + | Chr7:23207180-23207199 | MsG0780037291.01.T01:intron | 40.0% | |
| GCGTGAATGTAATCATGTGT+GGG | + | Chr7:23207181-23207200 | MsG0780037291.01.T01:intron | 40.0% | |
| TCATCATCTGATGCAGCATA+AGG | - | Chr7:23207389-23207408 | None:intergenic | 40.0% | |
| TCCAGAATCAAAGAAAGAGC+TGG | + | Chr7:23207295-23207314 | MsG0780037291.01.T01:CDS | 40.0% | |
| TCCAGCTCTTTCTTTGATTC+TGG | - | Chr7:23207299-23207318 | None:intergenic | 40.0% | |
| CCTTGTCAAATGCATCAACC+AGG | - | Chr7:23207268-23207287 | None:intergenic | 45.0% | |
| CTTCTTGCTGATGAGTCACT+AGG | + | Chr7:23207323-23207342 | MsG0780037291.01.T01:CDS | 45.0% | |
| ! | CCTGGTTGATGCATTTGACA+AGG | + | Chr7:23207265-23207284 | MsG0780037291.01.T01:CDS | 45.0% |
| GGTGGATGCTGTAGTCTACC+TGG | + | Chr7:23207247-23207266 | MsG0780037291.01.T01:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 23207115 | 23207430 | 23207115 | ID=MsG0780037291.01;Name=MsG0780037291.01 |
| Chr7 | mRNA | 23207115 | 23207430 | 23207115 | ID=MsG0780037291.01.T01;Parent=MsG0780037291.01;Name=MsG0780037291.01.T01;_AED=0.56;_eAED=0.57;_QI=0|0|0|0.5|1|1|2|0|73 |
| Chr7 | exon | 23207115 | 23207153 | 23207115 | ID=MsG0780037291.01.T01:exon:3681;Parent=MsG0780037291.01.T01 |
| Chr7 | exon | 23207248 | 23207430 | 23207248 | ID=MsG0780037291.01.T01:exon:3680;Parent=MsG0780037291.01.T01 |
| Chr7 | CDS | 23207115 | 23207153 | 23207115 | ID=MsG0780037291.01.T01:cds;Parent=MsG0780037291.01.T01 |
| Chr7 | CDS | 23207248 | 23207430 | 23207248 | ID=MsG0780037291.01.T01:cds;Parent=MsG0780037291.01.T01 |
| Gene Sequence |
| Protein sequence |
